Bibliografias temáticas / Shoot regeneration

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Literatura científica selecionada sobre o tema "Shoot regeneration"

Autor: Grafiati
Publicado: 4 de junho de 2021
Última modificação: 1 de fevereiro de 2022

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Artigos de revistas sobre o assunto "Shoot regeneration"

1

Tezuka, Takahiro, Masashi Harada, Masahumi Johkan, Satoshi Yamasaki, Hideyuki Tanaka e Masayuki Oda. "Effects of Auxin and Cytokinin on In Vivo Adventitious Shoot Regeneration from Decapitated Tomato Plants". HortScience 46, n.º 12 (dezembro de 2011): 1661–65. http://dx.doi.org/10.21273/hortsci.46.12.1661.

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Adventitious shoots can be regenerated from the cut surface of the primary shoot and lateral branches in decapitated plants in vivo. This inherent regenerative ability of plants is useful for mass propagation. In the present study, we conducted histological observations of shoot regeneration and applied auxin and cytokinin to decapitated seedlings in four tomato cultivars. The cultivars produced different numbers of adventitious shoots after decapitation; ‘Petit’ produced the largest number of adventitious shoots (78.5 ± 10.2) and ‘Momotaro’ produced the fewest (12.1 ± 3.3). Histological observation of ‘Petit’ revealed that adventitious shoots regenerated from calli formed at the cut surface of stems. Adventitious shoot formation was inhibited by the presence of lateral branches. Shoot regeneration was prevented by application of 1-naphthaleneacetic acid to ‘Petit’. Application of 6-benzyladenine promoted shoot regeneration in ‘Momotaro’. These results suggest auxin synthesized de novo from the lateral branches inhibited shoot regeneration after decapitation and endogenous cytokinin might stimulate shoot regeneration. Chemical names: 1-naphthaleneacetic acid (NAA); 6-benzyladenine (BA)
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Cao, X., e F. Hammerschlag. "307 Growth Regulator Pretreatments Significantly Enhance the Efficiency of Shoot Organogenesis from Leaf Explants of Highbush Blueberry Cultivar Bluecrop". HortScience 35, n.º 3 (junho de 2000): 445A—445. http://dx.doi.org/10.21273/hortsci.35.3.445a.

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As part of a program to develop transgenic highbush blueberry (Vaccinium corymbosum L.) cultivars, studies were conducted to determine optimum conditions for high-efficiency shoot regeneration from leaf explants of in vitro propagated, commercially important, tissue culture-recalcitrant `Bluecrop' shoot cultures. The effects of pretreatments, growth regulators, and age of explant source on shoot organogenesis were investigated. A maximum of 98% shoot regeneration and 10 shoots regenerating per leaf explant occurred when explants of 2-week-old shoot cultures were incubated in the dark (for a total of 14 days) on pretreatment medium #1 containing 2.6 μM NAA and 5 μM TDZ for 4 days, next on pretreatment medium #2 containing 2.6 μM NAA and 7 μM zeatin riboside for 3 days, then on regeneration medium containing 1 μM TDZ for 6 weeks, and last on medium without growth regulators for 10 days. No shoot regeneration occurred if explants were incubated without exposure to pretreatments before incubation on regeneration medium. There were no significant differences in percentage of regeneration or the number of shoots regenerating per explant from leaf explants derived from either 1-, 2-, or 3-week-old shoot cultures. Shoot production per explant on 1 μM TDZ was about three times that on either 0.5 μM TDZ or 20 μM zeatin riboside, and nine times that on 5 μM TDZ.
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Chae, Soo Cheon, Haeng Hoon Kim e Sang Un Park. "Ethylene Inhibitors Enhance Shoot Organogenesis of Gloxinia (Sinningia speciosa)". Scientific World Journal 2012 (2012): 1–4. http://dx.doi.org/10.1100/2012/859381.

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Shoot organogenesis and plant regeneration inSinningia speciosawere improved using ethylene inhibitors. The leaf explants were cultured on initial shoot regeneration media (MS media with BAP at 2 mg/L + NAA at 0.1 mg/L) supplemented with different concentrations of aminoethoxyvinylglycine (AVG), cobalt chloride (CoCl2), and silver thiosulphate (STS). The addition of AVG, CoCl2, and STS significantly improved the regeneration frequency giving higher shoots per explant and longer shoot length. The highest shoot growth was found when STS at 5 mg/L was incorporated with generation medium, performing highest regeneration frequency with highest number of shoots. This treatment (STS at 5 mg/L) produced 40% more shoots per explant compared to control followed by STS at 10 mg/L with increasing 37% more shoots compared to control. In the cases of AVG and CoCl2the highest shoot number per explant was found at 1 mg/L. Treated with AVG and CoCl2at 1 mg/L increased shoot number by 16 and 12%, respectively, compared to control. Ethylene inhibitors could be used as a possible micropropagation and plant transformation protocol inS. speciosafor plant regenerations.
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Xu, L., G. F. Liu e M. Z. Bao. "Adventitious Shoot Regeneration from In Vitro Leaves of Formosan Sweetgum (Liquidambar formosana L.)". HortScience 42, n.º 3 (junho de 2007): 721–23. http://dx.doi.org/10.21273/hortsci.42.3.721.

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Plantlets were regenerated from in vitro-grown leaf explants of five genotypes of Liquidambar formosana on WPM basal medium supplemented with different concentrations of TDZ and NAA. With the addition of 0.27 μm NAA, regeneration efficiency was increased by 2- to 4-fold over that with TDZ alone. Lower concentrations of TDZ (0.45–2.27 μm) were beneficial for regenerating shoot clusters. Four genotypes (P2, P6, P9, and P11) showed high regeneration rates (up to 90%), whereas genotype P13 showed a low capability for shoot regeneration on all media tested (<35%). For all five genotypes, the optimum medium for inducing adventitious shoots was WPM supplemented with 1.14 μm TDZ and 0.27 μm NAA, on which regeneration rate ranged from 72.6% to 89.5% and adventitious shoot clusters per regenerating leaf explant ranged from 2.63 to 3.11 in four genotypes (P2, P6, P9, and P11), while for P13, the regeneration rate and number of shoot clusters per regenerating explant were 23% and 1.39, respectively. Transfer of shoot clusters to WPM basal medium containing 0.54 μm NAA, 2.22 μm BA, and 1.44 μm GA3, resulted in shoot elongation. All the elongated shoots were rooted on WPM supplemented with 9.84 μm IBA, and plantlets were transplanted to soil successfully. Chemical names used: 6-benzyladenine (BA), gibberellic acid (GA3), indole-3-butyric acid (IBA), 1-naphthalene acetic acid (NAA), plant growth regulator (PGR), thidiazuron (TDZ), woody plant medium (WPM).
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Brand, Mark H. "099 Indirect and Direct Regeneration of Kalmia latifolia". HortScience 34, n.º 3 (junho de 1999): 458D—458. http://dx.doi.org/10.21273/hortsci.34.3.458d.

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To introduce desirable trait genes into Kalmia latifolia, efficient adventitious shoot regeneration methods are needed. Silver Dollar (S$) callus induction and growth in the dark was compared on Woody Plant (WP) medium containing 2,4-dichlorophenoxyacetic acid (2,4-D) (1, 5, 10, 20 μM) or naphthaleneacetic acid (NAA) (1, 10, 20, 40 μM) with and without 5 μM isopentenyladenine (2iP). Both 2,4-D and NAA produced >450 mg of callus from leaf explants in 8 weeks. The addition of 2iP tripled growth for 2,4-D and doubled growth for NAA. Greatest callus growth was obtained on 20-40 μM NAA or 5-20 μM 2,4-D. Shoot regeneration on callus was achieved on WP medium containing 30 μM 2iP or 1 μM thidiazuron (TDZ), but a combination of the two was best, with 68% of dark-grown calli regenerating shoots in 4 weeks. 26% more dark-grown calli regenerated shoots than light-grown calli. The type of auxin (2,4-D or NAA) used to grow the calli did not affect shoot regeneration. For direct shoot regeneration, S$ leaf explants were tested on WP medium containing 5, 15, 30, 45 and 60 μM 2iP. The addition of 1 μM indole-3-butyric acid (IBA) doubled the percentage of leaves that regenerated shoots. 2iP concentrations between 15 and 45 μM supported excellent shoot regeneration, but optimal regeneration (95% of explants, 5.1 shoots/leaf) occurred on 30 μM 2iP+1 μM IBA. Leaf explants of six cultivars were grown on optimal medium with shoot regeneration ranging from 17% to 93% of leaves and 1.8 to 8.2 shoots per leaf, depending on the cultivar.
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Orlikowska, Teresa, Agnieszka Marasek e Danuta Kucharska. "Regeneration of Paeonia mlokosewitschii Lom. and P. tenuifolia L. in vitro from different explants". Acta Societatis Botanicorum Poloniae 67, n.º 3-4 (2014): 223–27. http://dx.doi.org/10.5586/asbp.1998.026.

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The pattern of regeneration from tissues of <em>Paeonia mlokosewitschii</em> and <em>P. tenuifolia</em> cultured in vitro in the same chemical conditions depended on the initial explant. Direct shoot regeneration was obtained from the bases of petioles and petals, and leaf veins. Vegetative initial buds and regenerated in vitro shoots produced on their bases slowly growing nodular callus which was very productive in repetitive shoot regeneration. The tops of stems, flower bases, sepals, petals and ovary walls produced small callus which regenerated white and red spherical structures within 1.5 years. After that time also from those cultures arised nodular, shoot regenerating callus developed.
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George, M. W., e R. R. Tripepi. "084 Plant Preservative Mixture (PPM) can Reduce Shoot Regeneration from Leaf Explants of Selected Plants". HortScience 34, n.º 3 (junho de 1999): 455E—455. http://dx.doi.org/10.21273/hortsci.34.3.455e.

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Plant preservative mixture (PPM) is a new broad-spectrum biocide that may be useful for plant tissue culture. The objective of this study was to determine if PPM interfered with adventitious shoot regeneration on leaf explants from several plant species. Leaf explants from Dendranthema grandiflora `Iridon', Betula pendula, Rhododendron catawbiense var. album and R.c. `America' were made from the top two apical leaves on the microshoots. In the first experiment, 0, 0.5, 1, 2, or 4 mL·L-1 PPM were added to species-appropriate regeneration media. In the second experiment, only mum leaf explants were placed on regeneration media containing 0, 0.1, 0.2, 0.3, or 0.4 mL·L-1 PPM. The percentage of explants forming shoots and the number of shoots per regenerating explant were recorded after 4, 6, and 10 weeks, for mum, birch, and rhododendron leaves, respectively. The percentages of shoot regeneration from birch and rhododendron leaf explants were unaffected by up to 4 mL·L-1 PPM, and the number of shoots formed per R.c. album explant were also unaffected by the tested concentrations of PPM. In contrast, the numbers of shoots formed on birch and `America' explants were reduced 48% and 25%, respectively, when 4 mL·L-1 PPM was used in the media. The percentages of shoot regeneration and number of shoots per explant were drastically reduced on mum explants when only 0.5 mL·L-1 PPM was used in the medium. In fact, 0.3 mL·L-1 PPM or higher reduced shoot formation by more than 5-fold. This study demonstrates that the effects of PPM on shoot regeneration from leaf explants are species specific.
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Hebert, Cary J., Darren H. Touchell, Thomas G. Ranney e Anthony V. LeBude. "In Vitro Shoot Regeneration and Polyploid Induction of Rhododendron ‘Fragrantissimum Improved’". HortScience 45, n.º 5 (maio de 2010): 801–4. http://dx.doi.org/10.21273/hortsci.45.5.801.

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Rhododendron L.‘Fragrantissimum Improved’ is an attractive cultivar with showy, fragrant flowers but has limited potential for breeding because it is a sterile wide hybrid. Protocols for in vitro regeneration and polyploid induction were developed for this cultivar as a means to potentially restore fertility and enhance ornamental traits. Combinations of thidiazuron (TDZ) at 0, 5, 10, 15, or 20 μM and 1-naphthaleneacetic acid (NAA) at 0, 2.5, 5, or 10 μM were used to induce shoot regeneration from leaves. Shoot regeneration was optimized (68% of leaf segments produced shoots) using 8.8 μM TDZ and 10 μM NAA. To induce polyploidy, regenerative callus was treated with 7.5, 15, 30, 60, or 90 μM of the mitotic inhibitor oryzalin for 1, 3, 5, 7, or 14 d in various combinations. Oryzalin significantly affected survival and shoot regenerative capacity. A percentage of homogenous, tetraploid shoots was recovered from treatments of 30 μM oryzalin for 1 (13%) or 3 (13%) days and 7.5 μM oryzalin for 7 (20%) or 14 (7%) days.
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Cao, Xiaoling, Freddi A. Hammerschlag e Larry Douglass. "A Two-step Pretreatment Significantly Enhances Shoot Organogenesis from Leaf Explants of Highbush Blueberry cv. Bluecrop". HortScience 37, n.º 5 (agosto de 2002): 819–21. http://dx.doi.org/10.21273/hortsci.37.5.819.

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As part of a program to improve highbush blueberry (Vaccinium corymbosum L.) cultivars via tissue culture and genetic engineering, studies were conducted to determine optimum conditions for organogenesis from leaf explants of the previously recalcitrant cv. Bluecrop. The effects of a pretreatment, growth regulators, and age of explant source on shoot organogenesis were investigated. A maximum of 98% explants regenerated shoots with a mean of 11 shoots per leaf explant after 62 days when explants of 2-week-old shoot cultures were incubated on the following regime: pretreatment medium #1 containing 5 μm TDZ and 2.6 μm NAA for 4 days, pretreatment medium #2 containing 7 μm zeatin riboside and 2.6 μm NAA for 3 days, regeneration medium containing 1 μm TDZ for 6 weeks, and last on medium without growth regulators for 10 days. No shoot regeneration occurred if explants were incubated without exposure to pretreatment prior to incubation on regeneration medium. There were no significant differences in percentage of regeneration or the number of shoots regenerating per explant from leaf explants derived from either 1-, 2-, or 3-week-old shoot cultures. Shoot production per explant on regeneration medium containing 1 μm TDZ was about three times that on 0.5 μm TDZ or 20 μm zeatin riboside, and nine times that on 5 μm TDZ. Chemical names used: 1-phenyl-3-(1,2,3-thiadiazol-5-yl)urea (thidiazuron, TDZ); 9-(β-D-ribofuranosyl)-6-(4-hydroxy-3-methyl-but-2-enylamino)purine (zeatin riboside).
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Stamp, James A., Sheila M. Colby e Carole P. Meredith. "Improved Shoot Organogenesis from Leaves of Grape". Journal of the American Society for Horticultural Science 115, n.º 6 (novembro de 1990): 1038–42. http://dx.doi.org/10.21273/jashs.115.6.1038.

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Adventitious shoots developed within 3 weeks from the petiolar stub and, less often, from wounded lamina tissues when leaves excised from nodal cultures of Vitis vinifera L. cvs. French Colombard and Thompson Seedless were cultured on solid Nitsch and Nitsch medium containing BAP at 2 mg·liter-1. The youngest leaf that could be excised, from 1 to 8 mm long, was the most responsive (90% of explants producing shoots compared to 16% for leaf 6). Removal of the lamina from the petiolar stub within the first 3 weeks of culture reduced shoot production. Increase in nodal culture age, without transfer to fresh medium, had no effect on subsequent regeneration from the youngest leaves but did reduce the regeneration frequency of leaves at the next position from 43% to 20%. In regularly subculture nodal cultures, the number of transfers had no effect on subsequent regeneration. Leaves from recently established shoot tip cultures were more responsive than leaves from nodal cultures. The frequency of shoot production was higher in laterally bisected than intact leaves (70% vs. 43%) due to additional regeneration from the distal leaf half at the sites of severed veins. Shoot outgrowth was promoted by the isolation and subculture of regenerating tissue to fresh regeneration medium. Petiolar stub removal promoted de novo shoot organogenesis from the resulting lamina wound. Shoots rooted at a high frequency on Murashige and Skoog medium with 1 mg IA-A/liter and produced morphologically normal plants. Chemical names used: 6-benzylaminopurine (BAP); indole-3-acetic acid (IAA).
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Mais fontes

Teses / dissertações sobre o assunto "Shoot regeneration"

1

Kanakis, Andreas G. "In vitro plant regeneration studies with Capsicum annuum". Thesis, University of Bath, 1987. https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.380371.

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2

Boul, H. Lawrence. "A study of shoot regeneration in leaf disc cultures of two solanaceous plants". Thesis, University of Canterbury. Botany, 1992. http://hdl.handle.net/10092/5678.

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The behaviour of Solanum laciniatum and "Mitchell" Petunia leaf discs in culture was examined. In both systems shoots were formed in response to an appropriate auxin/cytokinin balance via a caulogenic pathway involving the epidermal tissues with no intermediate callus. Petunia explants could also be induced to form roots, which were found to have an endogenous origin quite different to that observed for shoots. The shoot inducing hormone balance was found to be required for only a short window in the shoot formation timecourse. The hormone balance required to make explants competent for shoot initiation, was less specific than that required for them to become determined for shoot development. Development itself would occur on a medium devoid of growth regulators. The effect of culturing explants on ungelled medium was also examined. The number of shoots initiated was dramatically increased over those on agar gelled media for both species. Liquid cultured Petunia explants showed an increase in both the number of shoots developing per explant and the rate of development. The effects of a number of reagents on shoot formation from Solanum laciniatum and Petunia explants was examined as was the effect of the nitrogen source on Petunia explants. The response of explants of both species to 2,3,5 triiodobenzoic acid (TIBA) , ribose, sorbitol and acetylsalicylic acid (ASA) was similar although the sensitivities varied. TIBA was found to promote shoot formation at low concentrations, but inhibit it at higher levels. Ribose, sorbitol and ASA inhibited shoot formation from explants of both species, and partial substitution of Murashige and Skoog nitrogen with NH4Cl inhibited shoot formation from explants of Petunia. Changes in gene expression accompanying shoot, root and callus production in Petunia explants were monitored by two dimensional polyacrylamide gel electrophoresis of both total extractable protein, and labelled newly synthesised proteins (for shoot and callus forming cultures). In addition the protein profiles of shoot forming cultures inhibited with ASA, TIBA and NH4Cl were also examined. A single silver stainable protein specific to shoot forming cultures, and 4 proteins specific to root formation were discovered. In general, protein changes in the first 4 days of culture greatly exceeded subsequent changes for all three culture systems. The patterns of proteins observed from explants cultured on the three inhibitors were similar. The inhibitors did not prevent the formation of the shoot specific protein, but did result in other changes in protein profile. The most significant of these was the occurrence of a labelled protein which was also found to occur in the nonshoot-forming central portion of uninhibited explants.
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Barretto, Sherwin Savio. "Tobacco shoot regeneration from calli in temporary immersion culture for biosynthesis of heterologous biopharmaceuticals". Thesis, Imperial College London, 2014. http://hdl.handle.net/10044/1/44957.

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‘Molecular farming’, the use of transgenic plants to produce biopharmaceutical proteins is emerging as a new biotechnological paradigm. Transgenic plants offer several advantages over conventional microbial and mammalian cell host technologies. In particular, transplastomic plants, with transformed plastid genomes, are capable of massive expression of foreign proteins and represent a promising platform for biopharmaceutical synthesis. The main theme of this PhD thesis is the investigation of in vitro regeneration of tobacco (Nicotiana tabacum) shoots from callus tissue in temporary immersion (TI) culture for heterologous biopharmaceutical synthesis. There is special emphasis on subunit vaccine expression in transplastomic tobacco, in which foreign protein accumulation is correlated with chloroplast number and development during the organogenesis process. Studies using transplastomic N. tabacum expressing TetC (tetanus toxin fragment C) investigated the influence of several culture parameters on biomass regeneration and recombinant protein expression. The parameters investigated include medium nitrogen source ratio, sucrose concentration and hydrodynamics. These studies highlight the sensitivity of transplastomic protein yields to the culture microenvironment, and provide a starting point for further optimisation. Further studies demonstrated the feasibility of TI culture for biosynthesis of proteolytically-unstable transplastomic subunit vaccines, p24 (HIV antigen) and VP6 (rotavirus antigen). TI culture is also demonstrated as a means for nuclear expression of functional Guy’s 13 monoclonal antibody. Finally, the use of TI culture as the basis of novel technological innovations is investigated. This includes the demonstration of transplastomic protein expression in a prototype large-scale mechanical temporary immersion bioreactor. Encapsulation of callus aggregates in an alginate matrix for long-term germplasm preservation was trialled, prior to temporary immersion regeneration. Overall, this work presents a novel in vitro propagation method for the contained large-scale biosynthesis of biopharmaceutical proteins, as a potential alternative to conventional plant propagation platforms based on agricultural cultivation or cell suspension culture.
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Craig, Jared Matthew. "EFFECTS OF MIDSTORY REMOVAL AND SHOOT CLIPPING ON THE GROWTH AND DEVELOPMENT OF THREE OAK SPECIES". UKnowledge, 2012. http://uknowledge.uky.edu/forestry_etds/9.

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Problems developing tall oak seedlings of high abundance have become a concern throughout many eastern hardwood forests. The decline in oak seedling recruitment into canopy positions is often attributed to the increasing abundance of shade tolerant midstory species, especially red maple (Acer rubrum L.). Studies have shown that increasing light to the understory by way of a midstory removal has the ability to favor oak seedlings over competitors. The majority of studies to date have examined northern red (Quercus rubra L.) and cherrybark oak (Quercus pagoda Raf.) on productive sites, but relatively little is known about the effects of midstory removal on white (Quercus alba L.) and black (Quercus velutina L.) oaks, which are valuable species commercially and for wildlife. This study tests the effect of a midstory removal on oak seedlings and red maples six years after treatment implementation. In addition to seedling growth, survival, and competitiveness, the study also illustrates the changes in canopy structure and light transmittance resulting from the midstory removal. Basal clipping response of white oak seedlings following six years under a midstory removal is also examined as a method for regenerating more vigorous oaks. Results from this study support implementation of midstory removal as a method for improving oak regeneration.
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Baloglu, Cengiz Mehmet. "Optimization Of Regeneration And Agrobacterium Mediated Transformation Of Sugar Beet (beta Vulgaris L.)". Master's thesis, METU, 2005. http://etd.lib.metu.edu.tr/upload/3/12606476/index.pdf.

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In this study, optimization of a transformation and regeneration system via indirect and direct organogenesis in cotyledon, hypocotyl, petiole, leaf and shoot base tissues of sugar beet (Beta vulgaris L. cv. ELK 345 and 1195) was investigated. Two different germination, three different callus induction and shoot induction medium was used for indirect organogenesis of sugar beet cultivar ELK 345. Except cotyledon, other explants (hypocotyl, petiole and leaf) produced callus. However no shoot development was observed from callus of these explants. Shoot base tissue of sugar beet cultivar 1195 was employed for direct organogenesis. Shoot development was achieved via direct organogenesis using 0.1 mg/L IBA and 0.25 mg/L BA. Root development and high acclimatization rate were accomplished from shoot base tissue. Different concentrations of kanamycin and PPT were applied to leaf blade explants to find out optimum dose for selection of transformants. Kanamycin at 150 mg/L and PPT at 3 mg/L totally inhibited shoot development from leaf blades. Moreover, an Agrobacterium mediated transformation procedure for leaf explants of ELK 345 was also optimized by monitoring transient uidA expression 3rd days after transformation. Effects of different parameters (vacuum infiltration, bacterial growth medium, inoculation time with bacteria, Agrobacterium strains and L-cysteine application in co-cultivation medium) were investigated to improve transformation procedure. Vacuum infiltration and Agrobacterium strains were significantly improved transformation procedure. Percentage of GUS expressing areas on leaves increased three folds from the beginning of the study.
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Kocer, Zeynep Ahsen. "In Vitro Induction Of Growth And Development Of Common Juniper (juniperus Communis L.) From Shoot And Bud Explants". Master's thesis, METU, 2005. http://etd.lib.metu.edu.tr/upload/3/12605891/index.pdf.

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The objective of the study was to investigate the optimum conditions for in vitro regeneration of common juniper (Juniperus communis L.) by using indirect organogenesis approach. Throughout the study
callus induction, organogenesis, improved organogenesis and root induction experiments were performed sequentially. It was found that explant position, genotype, gender, treatments and sampling time had significant effects on callus induction rate in common juniper. The results of treatments indicated that IBA (indole-3-butyric acid) at concentration range 0.5-4.0 mg/l combined with MS medium supplemented with 0.1 mg/l BAP (benzylaminopurine), 3 % sucrose and 0.7% agar was the best one among the treatments to induce callus formation from common juniper explants collected as Spring buds. Also, a two-month culture was adequate period for the callus induction of common juniper regardless of position, before transferring the explants into organogenesis media. After a two-month culture in callus induction media, explants were transferred to organogenesis treatments in order to investigate adventitious bud development from callus tissues. There were significant differences among genotypes, treatments and explant-sampling times in initiation of organ development in common juniper. Additionally, it was found that excluding the auxin components while maintaining 1.0-2.0 mg/l BAP concentration in culture media, as refreshing after a month, stimulated the formation and development of adventitious buds and shoots. Among the treatments tested, it was found that 1.0 mg/l BAP plus 0.5 mg/l 2,4-D was the optimum culture media with adventitious bud formation capacity of 37.5% was though ageing of callus significantly affected the frequency of adventitious bud formation. Finally, rooting experiments were performed to investigate rooting efficiency of adventitious shoots. In the adventitious rooting experiments, no rooting was observed in any of the treatments used with common juniper explants. Although whole plantlet development from callus tissues could not be achieved as indirect organogenesis, the results of the study could aid to future studies dealing in vitro regeneration and production of secondary chemicals from common juniper.
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Notini, Marcela Morato. "Understanding hormonal and temporal factors associated with tomato (Solanum lycopersicum L. cv. Micro-Tom) acquisition of competence: key concepts for in vitro shoot regeneration". Universidade de São Paulo, 2017. http://www.teses.usp.br/teses/disponiveis/11/11144/tde-07032018-132615/.

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Plant regeneration through de novo organogenesis is a critical step in most of the plant micropropagation and genetic transformation procedures. In the last years, significant progress has been made in the understanding of the mechanisms underlying de novo organogenesis in the worldwide crop tomato (Solanum lycopersicum). However, the hormonal and molecular factors involving the acquisition of competence for tomato shoot formation, an essential step for the regeneration process, are still not known. The failure in acquire competence can be the reason for the widely described absence of shoot regeneration from tomato root explants. In the first chapter, we conducted a temporal and hormonal characterization of the tomato acquisition of competence and the shoot induction phases using the model system cv. Micro-Tom. Regeneration was improved by pre-incubation on root-inducing medium (RIM) during the early two days in culture, a period corresponding to acquisition of competence step in cotyledon explants. Conversely, the pre-incubation on another auxin-rich condition, the callus-inducing medium (CIM), under the same period, abolished the regeneration achievement. The 2d RIM pre-treatment induced an extensive and intense endogenous auxin response in the explant, probably improving the cells competence to produce shoots under further cytokinin induction on shoot-inducing medium (SIM). This knowledge was applied to improve the Agrobacterium-mediated tomato genetic transformation procedure, leading to an efficient, simple, inexpensive and genotype-independent protocol. In the second chapter, we developed an unprecedented method for tomato shoot regeneration from root explants. The shoot organogenesis was obtained by adjusting the CIM pre-treatment to the acquisition of competence period, corresponding to the initial four days in culture for root explants. The number and quality of shoots formed were also augmented by the optimization of explants properties, medium components, and culture conditions. Taken the two chapters together, the knowledge obtained about organogenic competence advanced and created new regeneration and genetic transformation systems, which are very useful tools for biotechnology and functional studies of specific genes in tomato.
A regeneração de plantas através da organogênese de novo é uma fase crítica para a maioria dos procedimentos de micropropagação e transformação genética. Recentemente, progressos significativos tem sido alcançados no entendimento dos mecanismos fundamentais à organogênese de novo de tomateiro (Solanum lycopersicum). Entretanto, fatores hormonais e moleculares envolvidos na aquisição de competência para formação de gemas caulinares na espécie, etapa essencial ao processo de regeneração, permancece desconhecido. O fracasso em adquirir competência pode ser associado a amplamente descrita incapacidade de tomateiro em regenerar brotos caulinares a partir de raízes. No primeiro capítulo, realizou-se uma caracterização temporal e hormonal das fases de aquisição de competência e indução de gemas caulinares usando a cultivar modelo Micro-Tom. A eficiência de regeneração foi melhorada através de pré-incubação em meio indutor de raízes (RIM) durante os dois primeiros dias de cultivo, período correspondente à fase de aquisição de competência em explantes cotiledonares. Diferentemente, a pré-incubação em outro meio rico em auxina, o meio indutor de calo (CIM), sob mesmo intervalo, aboliu completamente a regeneração. A pré-incubação de dois dias em RIM induziu uma intensa e extensa resposta a auxina endógena no explante, o que provavelmente aumentou a competência das células a induzir brotos caulinares em resposta a citocinina presente no meio indutor de gemas caulinares (SIM). A aplicação desse conhecimento na melhoria do procedimento de transformação genética via Agrobacteria levou a um eficiente, simples, barato e genótipo-independente protocolo. No segundo capítulo, nós desenvolvemos um método inédito de regeneração de tomateiro via explante radicular. A formação de brotos caulinares foi obtida por ajuste do pré-tratamento em CIM ao período de aquisição de competência, correspondente a quatro dias de cultivo em explantes radiculares. O número e qualidade dos brotos também foram elevados pela otimização do explante, composição do meio de cultivo, e condições de cultivo. Somando-se os dois capítulos, o conhecimento obtido a cerca da competência organogênica resultou em novos sistemas de regeneração e transformação genética, ferramentas importantes para processos biotecnológicos e estudos funcionais de genes específicos em tomateiro.
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8

Aissa, Abdi Fatima. "Mitochondrial complex I dysfunction enhances in vitro plant organogenesis". Thesis, Université Paris-Saclay (ComUE), 2018. http://www.theses.fr/2018SACLS136/document.

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La régénération in vitro est un processus complexe largement utilisé pour la multiplication végétative ainsi qu'en recherche fondamentale pour étudier l'organogenèse. Malgré les diverses applications de la caulogenèse in vitro, les mécanismes de régulation impliqués restent mal caractérisés. Avant le début de mon doctorat, nous avons identifié un mutant d'Arabidopsis thaliana chez lequel un défaut du complexe I de la chaîne de transport d'électrons mitochondriale (CTEm) entraîne une augmentation du taux de régénération comparé au sauvage, mesurée sur des cals issus de protoplastes. Au début de mon projet doctoral, j'ai confirmé le lien entre le dysfonctionnement respiratoire et l'augmentation des taux de régénération en utilisant un inhibiteur spécifique du complexe I appelé roténone. Pour comprendre ce phénomène, j'ai étudié les mécanismes moléculaires et biochimiques liant la respiration mitochondriale et l'organogenèse in vitro. J'ai analysé différents mutants affectés dans l'activité du complexe I et conclu que le retard de croissance qui en découle est positivement corrélé avec le taux de régénération. Pour comprendre comment les perturbations de la CTEm affectent la formation des bourgeons, j'ai comparé les profils d'expression des gènes dans des tissus mutants du complexe I et dans des cals traités avec la roténone. Les résultats obtenus montrent, d’une part, que le profil d’expression des gènes est différent chez le sauvage et chez les mutants du complexe I et, d’autre part, que la roténone induit un stress oxydatif, inhibe la prolifération cellulaire et module les régulations hormonales. J'ai confirmé que la réponse oxydative induite par la roténone est rapidement relayée dans le cytosol en utilisant un bio-senseur de l’état redox cellulaire. Nos résultats suggèrent un lien de causalité entre un stress oxydatif induit par des perturbations respiratoires et la hausse du taux de régénération. Nos travaux pointent vers des méthodes alternatives pour améliorer l'efficacité de l'organogenèse in vitro par inhibition transitoire d'activités mitochondriales
In vitro shoot regeneration is a complex process routinely used for vegetative propagation and to study plant organogenesis. Despite multiple applications of in vitro shoot initiation, the regulatory mechanisms involved remain poorly understood. Prior to the beginning of my PhD thesis, we identified an Arabidopsis thaliana mutant in which a defect in the complex I of the mitochondrial electron transport chain (mETC) results in a higher shoot regeneration rate compared to wild type, measured on protoplast-derived calli. At the beginning of my PhD project, I confirmed the link between the respiratory defect and the shoot regeneration boost with a specific complex I inhibitor called rotenone. To understand this phenomenon, I investigated the molecular and biochemical mechanisms linking mitochondrial respiration and shoot organogenesis. For this purpose, I analyzed different mutants affected in the complex I activity and concluded that the resulting growth retardation is positively correlated with the regeneration rate. To understand how mETC perturbations promote shoot regeneration, I compared gene expression profiles in complex I mutant tissues and in calli treated with rotenone. Our data show, on the one hand, that gene expression profiles are different in complex I mutants and, on the other hand, that rotenone induces an oxidative stress, inhibits cell proliferation, and modulate hormonal regulations. I confirmed that the oxidative response induced by rotenone is rapidly relayed in the cytosol with a redox- sensitive biosensor. Altogether, our results suggest a causal link between an oxidative stress caused by respiratory impairments and shoot regeneration enhancement. Our findings point to alternative methods to promote in vitro organogenesis via transient inhibition of mitochondrial activities
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Rocha, Gabriel Henrique Braga. "Análise do papel da via miR156/SQUAMOSA Promoter-Binding Protein-Like (SPL) na organogênese in vitro a partir de raízes de Arabidopsis thaliana". Universidade de São Paulo, 2016. http://www.teses.usp.br/teses/disponiveis/11/11144/tde-17062016-180648/.

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Os microRNAs (miRNAs) são pequenos RNAs endógenos não codantes de 21-24 nucleotídeos (nt) que regulam a expressão gênica de genes-alvos. Eles estão envolvidos em diversos aspectos de desenvolvimento da planta, tanto na parte aérea, quanto no sistema radicular. Entre os miRNAs, o miRNA156 (miR156) regula a família de fatores de transcrição SQUAMOSA Promoter-Binding Protein-Like (SPL) afetando diferentes processos do desenvolvimento vegetal. Estudos recentes mostram que a via gênica miR156/SPL apresenta efeito positivo tanto no aumento da formação de raízes laterais, quanto no aumento de regeneração de brotos in vitro a partir de folhas e hipocótilos em Arabidopsis thaliana. Devido ao fato de que a origem da formação de raiz lateral e a regeneração in vitro de brotos a partir de raiz principal compartilham semelhanças anatômicas e moleculares, avaliou-se no presente estudo se a via miR156/SPL, da mesma forma que a partir de explantes aéreos, também é capaz de influenciar na regeneração de brotos in vitro a partir de explantes radiculares. Para tanto foram comparados taxa de regeneração, padrão de distribuição de auxina e citocinina, análises histológicas e histoquímicas das estruturas regeneradas em plantas com via miR156/SPL alterada, incluindo planta mutante hyl1, na qual a produção desse miRNA é severamente reduzida. Além disso, foi avaliado o padrão de expressão do miR156 e específicos genes SPL durante a regeneração de brotos in vitro a partir da raiz principal de Arabidopsis thaliana. No presente trabalho observou-se que a alteração da via gênica miR156/SPL é capaz de modular a capacidade de regeneração de brotos in vitro a partir de raiz principal de Arabidopsis thaliana e a distribuição de auxina e citocinina presente nas células e tecidos envolvidos no processo de regeneração. Plantas superexpressando o miR156 apresentaram redução no número de brotos regenerados, além de ter o plastochron reduzido quando comparado com plantas controle. Adicionalmente, plantas contento o gene SPL9 resistente à clivagem pelo miR156 (rSPL9) apresentaram severa redução na quantidade de brotos, além de terem o plastochron alongado. Interessantemente, plantas mutantes hyl1-2 e plantas rSPL10 não apresentaram regeneração de brotos ao longo da raiz principal, mas sim intensa formação de raízes laterais e protuberâncias, respectivamente, tendo essa última apresentado indícios de diferenciação celular precoce. Tomados em conjunto os dados sugerem que o miR156 apresenta importante papel no controle do processo de regeneração de brotos in vitro. Entretanto, esse efeito é mais complexo em regeneração in vitro a partir de raízes do que a partir de cotilédones ou hipocótilos.
MicroRNAs (miRNAs) are endogenous small non-coding RNAs of 21-24 nucleotides (nt) in length that regulate target gene expression. They are involved in many aspects of plant development, both in the shoot and in the root systems. Among miRNAs, miRNA156 (miR156) regulates SQUAMOSA Promoter Binding-Like (SPL) transcription factor family affecting different plant development processes. Recent studies have shown that the miR156/SPL pathway has a positive effect both in the increase of lateral root formation and regeneration of shoots from leaves and hypocotyls in Arabidopsis thaliana. Because the origin of lateral root formation and in vitro shoot regeneration from primary root share similar anatomical and molecular features, in the present study was evaluated whether the miR156/SPL pathway, in the same manner that from aerial explants, is also able to influence the in vitro shoot regeneration from root explants. For this, it was compared regeneration rates, distribution pattern of auxin and cytokinin, histological and histochemical analyses of the structures regenerated in plants in with the miR156/SPL pathway is modified, including the mutant hyl1-1, in which the biosynthesis of this miRNA is severely reduced. Besides that, it was evaluated the expression pattern of miR156 and specific SPL target genes during in vitro shoot regeneration from primary roots of Arabidopsis it was observed that the alteration on the miR156/SPL pathway is capable to modulate in vitro shoot regeneration from the primary root of Arabidopsis and the distribution of auxin and cytokinin at the tissues and cells involved in the regeneration process. Plants overexpressing the miR156a have shown reduction in the number of regenerated shoots, and displayed a reduction in plastochron when compared with wild type plants. Additionally, plants expressing cleavage-resistant form of SPL9 (rSPL9) presented severe reduction in the amount of shoots, and extended plastochron. Interestingly, mutant hyl1-2 and plants rSPL10 did not show any shoot regeneration along the root, but high formation of lateral roots and protuberances, respectively, having rSPL10 presented evidence of precocious cell differentiation. Taken together, these data suggest that de miR156 and SPLs have an important role in the control the in vitro shoot regeneration process. However, its effect is somehow more complex in roots than in cotyledons or hypocotyls.
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Raikar, Sanjeev Vencu. "Protoplast fusion of Lolium perenne and Lotus corniculatus for gene introgression". Phd thesis, Lincoln University. Bio-Protection and Ecology Division, 2007. http://theses.lincoln.ac.nz/public/adt-NZLIU20080214.105406/.

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Protoplast fusion of Lolium perenne and Lotus corniculatus for gene introgression by Sanjeev V. Raikar Lolium perenne is one of the most important forage crops globally and in New Zealand. Lotus corniculatus is a dicotyledonous forage that contains valuable traits such as high levels of condensed tannins, increased digestibility, and high nitrogen fixing abilities. However, conventional breeding between these two forage crops is impossible due to their markedly different taxonomic origin. Protoplast fusion (somatic hybridisation) provides an opportunity for gene introgression between these two species. This thesis describes the somatic hybridisation, the regeneration and the molecular analysis of the putative somatic hybrid plants obtained between L. perenne and L. corniculatus. Callus and cell suspensions of different cultivars of L. perenne were established from immature embryos and plants were regenerated from the callus. Of the 10 cultivars screened, cultivars Bronsyn and Canon had the highest percentage of callus induction at 36% each on 5 mg/L 2,4-D. Removal of the palea and lemma which form the seed coat was found to increase callus induction ability of the embryos. Plant regeneration from the callus was achieved when the callus was plated on LS medium supplemented with plant growth regulators at different concentrations. Variable responses to shoot regeneration was observed between the different cultivars with the cv Kingston having the lowest frequency of shoot formation (12%). Different factors affecting the protoplast isolation of L. perenne were investigated. The highest protoplast yield of 10×106 g-1FW was obtained when cell suspensions were used as the tissue source, with enzyme combination ‘A’ (Cellulase Onozuka RS 2%, Macerozyme R-10 1%, Driselase 0.5%, Pectolyase 0.2%), for 6 h incubation period in 0.6 M mannitol. Development of microcolonies was only achieved when protoplasts were plated on nitrocellulose membrane with a L. perenne feeder layer on PEL medium. All the shoots regenerated from the protoplast-derived calli were albino shoots. The highest protoplast yield (7×106 g-1FW) of L. corniculatus was achieved from cotyledons also with enzyme combination ‘A’ (Cellulase Onozuka RS 2%, Macerozyme R-10 1%, Driselase 0.5%, Pectolyase 0.2%), for 6 h incubation period in 0.6 M mannitol. The highest plating efficiency for L. corniculatus of 1.57 % was achieved when protoplasts were plated on nitrocellulose membrane with a L. perenne feeder layer on PEL medium. The highest frequency of shoot regeneration (46%) was achieved when calli were plated on LS medium with NAA (0.1 mg/L) and BA (0.1 mg/L). Protoplast fusion between L. perenne and L. corniculatus was performed using the asymmetric somatic hybridisation technique using PEG as the fusogen. L. perenne protoplasts were treated with 0.1 mM IOA for 15 min and L. corniculatus protoplasts were treated with UV at 0.15 J/cm2 for 10 min. Various parameters affecting the fusion percentage were investigated. Successful fusions were obtained when the fusions were conducted on a plastic surface with 35% PEG (3350 MW) for 25 min duration, followed by 100 mM calcium chloride treatment for 25 min. A total of 14 putative fusion colonies were recovered. Shoots were regenerated from 8 fusion colonies. Unexpectedly, the regenerated putative hybrid plants resembled L. corniculatus plants. The flow cytometric profile of the putative somatic hybrids resembled that of L. corniculatus. Molecular analysis using SD-AFLP, SCARs and Lolium specific chloroplast microsatellite markers suggest that the putative somatic hybrids could be L. corniculatus escapes from the asymmetric protoplast fusion process. This thesis details a novel Whole Genome Amplification technique for plants using Strand Displacement Amplification technique.
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Mais fontes

Livros sobre o assunto "Shoot regeneration"

1

Sears, Julia Seton. The Short Cut: Regeneration Through Fasting. Kessinger Publishing, LLC, 2007.

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2

Sears, Julia Seton. Fasting for Regeneration: The Short Cut. Mokelumne Hill Pr, 1993.

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3

THE SHORT CUT: Regeneration Through Fasting. Cosimo Classics, 2006.

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4

Sears, Julia Seton. Fasting for Regeneration: The Short Cut. Life Science Institute (FL), 1985.

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5

Simpson, Leanne. Short History of the Blockade: Giant Beavers, Diplomacy, and Regeneration in Nishnaabewin. University of Alberta Press, 2021.

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6

Shepperd, W. D. Response of aspen root suckers to regeneration methods and post-harvest protection. 1996.

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Capítulos de livros sobre o assunto "Shoot regeneration"

1

Lercari, B., S. Moscatelli, E. Ghirardi, R. Niceforo e L. Bertram. "Photocontrol of Shoot Regeneration from Hypocotyls of Tomato". In Plant Biotechnology and In Vitro Biology in the 21st Century, 69–72. Dordrecht: Springer Netherlands, 1999. http://dx.doi.org/10.1007/978-94-011-4661-6_15.

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2

Gantait, Saikat, e Monisha Mitra. "Role of Meta-topolin on in Vitro Shoot Regeneration: An Insight". In Meta-topolin: A Growth Regulator for Plant Biotechnology and Agriculture, 143–68. Singapore: Springer Singapore, 2021. http://dx.doi.org/10.1007/978-981-15-9046-7_12.

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Paulraj, Subramanian, e Edward C. Yeung. "Improved Shoot Regeneration from Root Explants Using an Abscisic Acid-Containing Medium". In Plant Cell Culture Protocols, 183–89. Totowa, NJ: Humana Press, 2012. http://dx.doi.org/10.1007/978-1-61779-818-4_15.

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Ernst, Stephen G., e Gary D. Coleman. "The Transition between Shoot Regeneration Competence and Callus Determination in Internodal Stem Explants of Populus deltoides". In Woody Plant Biotechnology, 23–29. New York, NY: Springer US, 1991. http://dx.doi.org/10.1007/978-1-4684-7932-4_3.

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Durham, Richard E., e Schuyler S. Korban. "Effects of explant size, pretreatment, and light intensity on shoot regeneration from in vitro-grown apple leaves". In Developments in Plant Breeding, 355–59. Dordrecht: Springer Netherlands, 1994. http://dx.doi.org/10.1007/978-94-011-0467-8_71.

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Bassi, Gino, e Ferdinando Cossio. "Simplified protocol for in vitro shoot regeneration from leaves of Prunus domestica L. (cv ‘Susina di Dro’)". In Developments in Plant Breeding, 361–63. Dordrecht: Springer Netherlands, 1994. http://dx.doi.org/10.1007/978-94-011-0467-8_72.

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Li, Wenbin, Jie Liu, Pat Masilamany, Jeff H. Taylor, Genlou Sun, Manilal William e K. Peter Pauls. "Molecular Markers Associated with Plant Regeneration from Shoot Meristem Cultures Derived from Germinated Corn (Zea mays L.) Seeds". In Plant Biotechnology 2002 and Beyond, 289–92. Dordrecht: Springer Netherlands, 2003. http://dx.doi.org/10.1007/978-94-017-2679-5_59.

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Moustakas, M., M. Tsimilli-Michael, L. Purnhauser, G. Ouzounidou, L. Bona e R. J. Strasser. "Effect of Cu(II) on Shoot Regeneration In triticum Aestivum Tissue Cultures Probed by the Fast Fluorescence (Jip-Test)". In Progress in Botanical Research, 199–205. Dordrecht: Springer Netherlands, 1998. http://dx.doi.org/10.1007/978-94-011-5274-7_44.

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Dimasi-Theriou, K., e A. Economou. "Effect of Cold Temperature on Shoot Regeneration in Vitro from Aged Cultures of GF-677 (Prunus Persica × Prunus Amygdalus)". In Plant Aging, 345–49. Boston, MA: Springer US, 1990. http://dx.doi.org/10.1007/978-1-4684-5760-5_43.

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Tzfira, T., O. Yarnitzky, A. Vainstein e A. Altman. "Highly Efficient Transformation and Regeneration of Transgenic Aspen Plants Through Shoot-Bud Formation in Root Culture, and Transformation of Pinus Halepensis". In Somatic Cell Genetics and Molecular Genetics of Trees, 125–30. Dordrecht: Springer Netherlands, 1996. http://dx.doi.org/10.1007/978-94-011-3983-0_17.

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Trabalhos de conferências sobre o assunto "Shoot regeneration"

1

Taipova, R. M., e B. R. Kuluev. "Agrobacterium-mediated transformation of Amaranthus cruentus L. epicotyls by the ARGOS-LIKE transgene". In 2nd International Scientific Conference "Plants and Microbes: the Future of Biotechnology". PLAMIC2020 Organizing committee, 2020. http://dx.doi.org/10.28983/plamic2020.244.

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The present study describes the results of our research in Agrobacterium-mediated transformation of epicotyl segments of Amaranthus cruentus variety “Bagryanyi” by the ARGOS-LIKE transgene of Arabidopsis thaliana controlled by the 35S promoter. For shoot regeneration from epicotyl segments after Agrobacterium-mediated transformation, Murashige-Skoog (MS) medium containing 13 μM 6-benzylaminopurine and 1 μM α-naphthylacetic acid was used. For the selection of transgenic shoots, 10 mg/L of hygromycin B was added to the MS medium.
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2

"Effects of Plant Growth Regulators on Shoot Regeneration and Callus Induction of Carica papaya L." In August 6-8, 2018 Pattaya (Thailand). Eminent Association of Pioneers, 2018. http://dx.doi.org/10.17758/eares3.c0818112.

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Mastuti, Retno, Aminatun Munawarti e Mufidatur Rosyidah. "The effect of tomato juices and bean sprout extracts on vitro shoot regeneration of Physalis angulata L." In 8TH INTERNATIONAL CONFERENCE ON GLOBAL RESOURCE CONSERVATION (ICGRC 2017): Green Campus Movement for Global Conservation. Author(s), 2017. http://dx.doi.org/10.1063/1.5012720.

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Sidik, Norrizah Jaafar, Saiyidah Nafisah Hashim, Yaseer Suhaimi Mohamad e Shamsiah Abdullah. "Effects of natural and synthetic cytokinin hormone on shoot regeneration of rockmelon (Cucumis melo) Glamour cv. by using nodal explants". In 2012 IEEE Symposium on Business, Engineering and Industrial Applications (ISBEIA). IEEE, 2012. http://dx.doi.org/10.1109/isbeia.2012.6422984.

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Emaikwu, Nehemiah, David Catalini, Jan Muehlbauer, Yunho Hwang, Ichiro Takeuchi e Reinhard Radermacher. "Development of a Cascade Elastocaloric Regenerator". In ASME 2019 13th International Conference on Energy Sustainability collocated with the ASME 2019 Heat Transfer Summer Conference. American Society of Mechanical Engineers, 2019. http://dx.doi.org/10.1115/es2019-3887.

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Abstract Heat pumps based on the vapor compression cycle account for a significant portion of energy use around the world. However, growing demands for energy efficient and environmentally friendly technologies have created a need for new space conditioning approaches. Novel systems which use elastocaloric material have shown potential to replace traditional vapor compression due to high energy efficiency and use of environmentally friendly, solid-state refrigerants. The solid-state refrigerants exhibit the elastocaloric effect, a phenomenon that occurs when metal alloys experience stress-induced reversible phase transformations resulting in latent heat release or absorption. Prototypes built in the Center for Environmental Energy Engineering have utilized the active elastocaloric regeneration (AER) operating method to develop high temperature gradients between the ends of a regenerative heat exchanger made of tubular elastocaloric material. Though this schema significantly increases the temperature span developed by elastocaloric cooling devices, the current heat pump design leads to temperature degradation as a result of conduction along the length of the tubes in the regenerator. The novel regenerator concept presented in this work mitigates that issue by using short, thermally insulated tubes layers which also enables fluid flow over external surface areas of the material.
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Al Hayyan, Abdul Jabbar, Patricia Maria Kurniawati, Noor Idha Handajani, Soenarnatalina Melaniani e Hiroaki Kimura. "Adoption Evaluation of Indonesian Language Short Form 36 Cross Cultural Adaptation on People With Musculoskeletal Pain". In International Meeting on Regenerative Medicine. SCITEPRESS - Science and Technology Publications, 2017. http://dx.doi.org/10.5220/0007321603590363.

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Sampson, Alana C., Eunna Chung e Marissa Nichole Rylander. "Thermal Stress Conditioning to Induce Osteogenic Protein Expression for Bone Regeneration". In ASME 2012 Summer Bioengineering Conference. American Society of Mechanical Engineers, 2012. http://dx.doi.org/10.1115/sbc2012-80940.

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Although bone has the intrinsic ability to “self-heal”, there are circumstances in which its regenerative capacity is limited or compromised, such as in critical bone defects. In these cases, the lack of osteogenic proteins at the wound site can prevent healing and external stimuli may be necessary to encourage bone growth [1]. Exogenous delivery of proteins and growth factors directly to the wound has been successful in bone regeneration, but is limited by the instability of the proteins and short half-lives. As a result, administration of multiple large doses of protein is necessary to retain a beneficial protein level. Due to these disadvantages, additional methods have been investigated to supply essential proteins to the bone defect [2].
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8

Vasilyev, Michael, Pallavi G. Patki, Lu Li e Taras I. Lakoba. "All-optical regeneration of multiple WDM channels (Conference Presentation)". In Metro and Data Center Optical Networks and Short-Reach Links II, editado por Madeleine Glick, Atul K. Srivastava e Youichi Akasaka. SPIE, 2019. http://dx.doi.org/10.1117/12.2513478.

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Wilson, David Gordon. "Effect of Face-Area Ratio on Heat-Exchanger Pressure Drops, Size and Weight". In ASME Turbo Expo 2009: Power for Land, Sea, and Air. ASMEDC, 2009. http://dx.doi.org/10.1115/gt2009-60350.

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Designers of heat exchangers of all types normally have several degrees of freedom even while meeting the specified effectiveness exactly. One freedom is that of choosing the face-area ratios for the two (or more) fluids. A principal reason for choosing face-area ratio is to arrive at desired pressure drops for the fluids. The lowest pressure drop is not always beneficial: a low pressure drop can produce highly non-uniform flow that would degrade heat-exchanger performance. Obviously a high pressure drop penalizes system performance directly. In this paper it is shown that choosing face-area ratio is a good tool up to a point, one at which penalties in the form of increased size and cost of the overall heat exchanger begin to outweigh the benefits. This paper reports studies on the effects of choosing face-area ratios on rotary regenerative heat exchangers, but most results are applicable to fixed-surface recuperative heat exchangers also. However, one significant difference between the two types is that gas-turbine regenerators have short flow lengths, the thickness of the disk or drum. A short flow length is a virtue, because it reduces the regenerator disk volume and mass. But the disk thickness must not be allowed to be reduced to the point where there is substantial “short-circuit” thermal conduction between the hot and cold faces of a regenerator. These and other aspects of heat-exchanger design are explored in general and by means of examples, and design guidelines are suggested.
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Pammi, V. A., S. Terrien, N. G. R. Broderick, B. Krauskopf e S. Barbay. "Short and Long Term Memory in Regenerative Spiking Micropillar Lasers". In 2019 Conference on Lasers and Electro-Optics Europe & European Quantum Electronics Conference (CLEO/Europe-EQEC). IEEE, 2019. http://dx.doi.org/10.1109/cleoe-eqec.2019.8873079.

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Yin, Yan. Final Report: A Multi-Channel Recirculating Loop Signal Regenerator for High Frequency Single-Shot Bunch Length Measurement, August 13, 1998 - March 17, 1999. Office of Scientific and Technical Information (OSTI), junho de 1999. http://dx.doi.org/10.2172/765220.

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