Literatura científica selecionada sobre o tema "Ruminants Feeding and feeds"
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Artigos de revistas sobre o assunto "Ruminants Feeding and feeds"
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Klein, Frank, Tony Lupo, Don Pielack, Mark Mozola, David Pinero, Scott Coates, Nancy Thiex, Christoph von Holst e Jim Drouillard. "Validation Study of a Lateral-Flow Immunoassay for Detection of Ruminant By-Product Material in Animal Feeds and Feed Ingredients: Performance-Tested MethodSM 010405". Journal of AOAC INTERNATIONAL 88, n.º 6 (1 de setembro de 2005): 1583–92. http://dx.doi.org/10.1093/jaoac/88.6.1583.
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Abstract An immunoassay with a lateral flow format has been developed for the detection of ruminant by-product material in animal feeds and feed ingredients. The test is designed for the analysis of animal feeds destined for feeding to ruminants to ensure that they do not contain ruminant by-products in violation of the ruminant-to-ruminant feed ban established by the U.S. Food and Drug Administration in 1997. This feed ban was established as a firewall against exposure of ruminant livestock animals to the prion agents responsible for neurological diseases such as bovine spongiform encephalopathy and scrapie. The test is designed for field use, e.g., at a feed mill, and yields a qualitative (presence/absence) result in 15–20 min. The objective of the study was to validate the lateral-flow test for detection of ruminant by-product material in a variety of finished animal feeds and feed ingredients. Results indicate that the test is specific for ruminant material and can detect as little as 1% ruminant material in these commodities.
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ZOIOPOULOS (Π. ΖΩΙΟΠΟΥΛΟΣ), P. "Feed Ban and BSE: the detection and identification of processed animal proteins in compound feeds". Journal of the Hellenic Veterinary Medical Society 62, n.º 1 (10 de novembro de 2017): 71. http://dx.doi.org/10.12681/jhvms.14838.
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Reference is made initially to the incidence of BSE within the EU territory and the reasons of the appearance of the disease i.e. the use of meat and bone meal in ruminant diets. In turn, the evolution of Community legislation in the sector of marketing of feeds of animal origin is described. Furthermore, the banning of feeding of mammalian protein to ruminants and the extension of prohibition to further animal proteins such as from other vertebrates and how this banning affects the feeding of the various animal species is reviewed. Finally, the importance of detecting and identifying the presence and amount of processed animal proteins in compound feed as well as feed materials is stressed and methods such as feed microscopy, immunoassay techniques, PCR, NIR and HPLC are discussed.
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Mir, P. S., T. A. McAllister, D. J. Gibb e E. K. Okine. "Dietary oil rich in polyunsaturated fatty acids for ruminants: Post-ruminal digesta characteristics and their implications on production". Canadian Journal of Animal Science 86, n.º 2 (1 de junho de 2006): 159–70. http://dx.doi.org/10.4141/a05-056.
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Maximizing the production of ruminants requires high intakes of feeds that are energy dense, therefore feeds that are usually high in starch. Under these conditions there can be significant passage of partially digested feed starch and fibre to the small intestine. These digesta components interact in a manner that can diminish post-ruminal digestion and absorption. However, in ruminants, the effects of digesta composition on causing physical constraints to digestion and absorption, via increased digesta viscosity have not been given adequate consideration. While elevation of digesta viscosity as a means of reducing rate of nutrient absorption and re-absorption of compounds detrimental to health is a valid approach in humans, digesta viscosity may reduce the production potential of livestock. Thus, there is merit in reviewing the feeding strategies that lead to increased viscosity and how its mitigation can be managed through inclusion of oil in ruminant diets. The interactions between digesta composition and the resultant viscosity with digestive physiology and production factors are examined. This review also highlights the associated benefits of dietary oil for ruminants, such as enhancement of bio-active lipids in ruminant products, health benefits directly to the animal, through decreasing incidence of liver abscesses and effects on production and possibly on the environment (reduction of enteropathogenic bacterial shedding and methane emissions). Key words: Ruminant, starch, digesta characteristics, viscosity, cholecystokinin
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Colombatto, D., D. P. Morgavi e F. L. Mould. "Feed enzymes for ruminants. The need for a rational screening system". Proceedings of the British Society of Animal Science 2002 (2002): 249–50. http://dx.doi.org/10.1017/s1752756200009005.
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Enzymes that degrade the plant cell wall, collectively termed cellulases and hemicellulases, have attracted considerable research efforts recently, because of their potential to be used as additives for animal feeds. Widely used in monogastric animals, mainly to remove antinutritional factors (Bedford, 2000), enzymes are increasingly used in ruminant diets to enhance feedstuff degradability, following results from feeding trials where positive responses in rumen digestion and animal production were observed (Beaucheminet al., 1995; Fenget al., 1996; Yanget al., 1999). Enzymes could also provide an environmentally friendly alternative to the use of antibiotics as feed additives for ruminants.
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ZOIOPOULOS (Π. ΖΩΪΟΠΟΥΛΟΣ), P. "The applications of biotechnology in animal feeding: A critical overview". Journal of the Hellenic Veterinary Medical Society 55, n.º 2 (6 de dezembro de 2017): 156. http://dx.doi.org/10.12681/jhvms.15186.
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An effort is made in this article to present the applications of biotechnology as regards the improvement of nutritive value and the development of novel feeds as well as the production of various types of feed additives. With respect to raw materials, quality upgrading by bio technological means has been attempted through lignocellulose breakdown or protein enrichment. In addition, reference is made to bioproteins and feeds derived from transgenic plants, particularly those resistant to herbicides. In turn, reference is made to aminoacids as products of industrial fermentation. Furthermore, various feed additives, products of biotechnology, such as enzymes, antibiotics, probiotics, but also means for depression of methanogenesis in the forestomachs of ruminants, as well as silage agents, like inoculants and enzymes, are commented upon. In certain cases, reference is made to the issue of efficacy of biotechnology products when used in animal nutrition and the subject of feed safety. Finally, because of the need for licensing these products in order to be circulated, reference is made to the provisions of Community legislation governing the marketing of feeds. The article concludes with some thoughts on the future of the sector of application of biotechnology in the case of animal nutrition.
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Owens, Fredric N. "356 Anomalies of Carbohydrate Digestion and Metabolism by Ruminants". Journal of Animal Science 99, Supplement_3 (8 de outubro de 2021): 196. http://dx.doi.org/10.1093/jas/skab235.355.
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Abstract Carbohydrate, derived either from plant cell walls (neutral detergent fiber; NDF) from fresh or conserved forages consumed by cows and growing cattle or from starch of grains and concentrates in commercial feedlot diets, provides most of the dietary energy for ruminant growth and production. The ruminants’ ability to harvest forage from remote, inaccessible locations, to scavenge energy from NDF of feeds and byproducts of grain largely indigestible by non-ruminants, and to employ non-protein N to form protein-rich, nutrient-dense foods for humans are key ecological advantages over non-ruminants. Outlined below are some topics needing research attention. First, feed composition tables invariably indicate that the digested energy (DE) of carbohydrates is 4.4 kilocalories per gram, yet the gross energy (GE) of purified carbohydrate polymers (starch; cellulose, glycogen) averages 4.18; monomers (hexoses, pentoses) average 3.74. Having DE exceed GE violates the first Law of Thermodynamics! Might this 5 percent discrepancy reflect underestimations of fecal lignin or of protein digestibility due to metabolic fecal N? Favoring feeds rich in carbohydrate, this discrepancy confounds formulation of least-cost diets. Secondly, site of digestion of carbohydrate alters its metabolizable energy (ME). Fermentation, though required to yield ME from NDF, generates methane and heat. In contrast, starches and sugars that escape ruminal fermentation, if digested in the small intestine, circumvent these losses increasing their relative ME. Unfortunately, when ruminally fermented, starch, either directly or via pH depression, reduces NDF fermentation; might ruminal NDF digestion be increased by feeding NDF plus buffer versus concentrate diet components asynchronously? Third, carbohydrate metabolites lost in urine (lactate, pentoses, hippuric acid) need further quantification. Finally, ruminally-formed metabolites (methylglyoxal, methane) adversely affect animal health and energetics. Ruminal modification (site- or time-targeted enzymes or inhibitors; altering the ruminal microbiome with pre- or probiotics, inoculants, nutraceuticals) should help enhance ruminant productivity and sustainability.
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Pent, Gabe J. "192 Towards Year-Round Grazing in the Southeastern U.S". Journal of Animal Science 98, Supplement_2 (1 de novembro de 2020): 67. http://dx.doi.org/10.1093/jas/skz397.153.
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Abstract Winter feeds costs for small ruminants exceeds 50% of the total cost of most small ruminant production systems in the Southeastern U.S. Keeping these feed costs low is one of the most effective and time-tested ways to improve farm profitability. While maintaining an appropriate stocking rate will be critical for sustaining long-term farm productivity, a suite of other management practices is available to assist in this objective. Installing appropriate fence and watering system infrastructure for managing grazing will be critical for improving harvest efficiency and stockpiling forages for utilization when forage growth is limited. Filling gaps in forage production may also be achieved through the strategic use of a number of forage species, including warm-season or cool-season forages and annual or perennial forages. Managing stored forages appropriately during storing and feeding will help minimize feed losses, while producing quality hay will reduce the need for supplementary feeds. With the adoption of these proven practices, sheep and goat production may be optimized by allowing them to harvest their own feed almost year-round in the Southeastern U.S.
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Mavromati, J., Z. Gjeta, E. Mavromati, V. Kolaneci e A. Molla. "Production systems and economics of sheep husbandry in southern Albania". Biotehnologija u stocarstvu 27, n.º 3 (2011): 1167–76. http://dx.doi.org/10.2298/bah1103167m.
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Small ruminants in Albania seem to have a great importance within the livestock sector. The small ruminants' populations count almost 2.7 million heads, out of which 1.8 million are sheep. The estimated value of the commercialized small livestock population is about 35 million Euros. The total value of the small ruminant population is estimated at 140 million Euros. More than 1.5 million heads of small ruminants are consumed each year in the local market. Import of small ruminant?s meat was at a level of 599 ton/year (2010 data). This study aimed the assessment of the production systems and economics of sheep husbandry in the southern regions of Albania. The different husbandry systems employed (transhumant, non transhumant, semi-intensive) showed only slight differences in terms of outputs, but not the expected differences regarding inputs (feeds) and corresponding costs (semi-intensive system). The economic assessment demonstrates very useful and interesting results. Overall Gross Margin per ewe and lambs at weaning was in the area of 6,369.00 ALL (1 Euro=136 Albanian Lek, ALL), the range of GM was enormous between different farms (3,042.6 ALL - 11,322.4 ALL), mainly caused by the large differences in feeding and labor costs per ewe.
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Radchikov, V. F., V. P. Tsai, V. K. Gurin e V. A. Lyundishev. "ORGANIC TRACE ELEMENT COMPLEX FOR CALVES FEEDING". Scientific Messenger of LNU of Veterinary Medicine and Biotechnologies 18, n.º 3(71) (13 de outubro de 2016): 225–27. http://dx.doi.org/10.15421/nvlvet7152.
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Trace elements are commonly used in the form of inorganic salts poorly digested by ruminants due rumen microflora, which transits its greater part into indigestible and insoluble form. Trace elements in the form of chelates are supplied with amino acids and peptides, and used by the body much more efficiently. Inclusion of organic trace element complex (OMEK) in compound feed KR–1 in the amount of 10% of the existing standards for trace elements content in typical formulations for growing young cattle for meat has a positive impact on palatability of feeds, morphological and biochemical composition of blood and animals’ performance, economic efficiency of calves fattening. Use of organic trace element complex in compound feeds KR–1 increases concentration of total protein in blood by 8 – 12% (P < 0.05), reduces the amount of urea by 11 – 16% (P < 0.05). Comparative analysis showed that animals in experimental group II used the feeds more efficiently. Metabolizable energy consumption per 1 kg of weight gain made 33.7 MJ against 37.4 MJ in the control group or by 9.9% lower, the same trend is established for digestible protein cost – by 10%. Inclusion of OMEK in compound feed KR–1 for young cattle ensures average daily weight gain increase by 12.3% (P < 0.05), decreased of feed cost per 1 kg of weight gain by 10%. Use of organic trace element complex helps to decrease the cost price of weight gain, by 9.0% and obtain extra profit of $37.2 USD per one animal during the experimental period.
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Deaville, E. R. "The role of biotechnology in enhancing the digestion of forage cell walls by ruminants (jerry hughes scholarship)". Proceedings of the British Society of Animal Science 1995 (março de 1995): 73. http://dx.doi.org/10.1017/s1752756200590693.
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The term biotechnology has been defined as the application of biological organisms, systems or processes to manufacture and service industries (Anon, 1980) and is, therefore, more than the application of ‘genetic engineering’ techniques alone. The potential application of biotechnology to the agricultural livestock industry includes many wide ranging areas: animal health; breeding; livestock production; livestock nutrition and the nutritive value of feeds. The role of biotechnology in animal nutrition and feeding is of particular importance since feed costs account for a significant proportion of the total variable costs in any livestock production system (e.g. milk, meat). The potential implications of biotechnology in animal nutrition has been reviewed by Armstrong (1986) and includes references to the improvement of the nutritive value of feeds through, for example, genetic manipulation of feed sources (cereals), appropriate supplementation and the use of biological inoculants with or without enzymes as silage additives and to improvements in the ability of the animal to obtain nutrients from feeds through the addition of enzymes to feeds and modification of rumen microbes through genetic engineering.
Teses / dissertações sobre o assunto "Ruminants Feeding and feeds"
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Llorente, Eduardo Gallo. "Ammonia and urea treatment of wheat straw for feeding to ruminants". Thesis, Virginia Polytechnic Institute and State University, 1986. http://hdl.handle.net/10919/91048.
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The effect of treatment with 3% aqueous ammonia and 5.3% urea plus jackbean meal on the nutritive value of wheat straw was investigated. The moisture content of the straw was raised to 40% by addition of water. Crude protein and in vitro dry matter digestibility were highest for the bottom layers, which had the highest moisture. The mean temperature during the first 4 wk post treatment was 11.2 C. Four diets were fed to sheep: untreated straw 1) alone or 2) with 20 g/d urea at feeding time 3) ammonia-treated straw, and 4) urea-treated straw. All diets contained 80 g of liquid molasses, 10 g of NaCl and 6 g of Na 2 S04, and were calculated to be isonitrogenous except the control diet (untreated straw without urea supplementation). Straw was chopped prior to feeding. Crude protein content in wheat straw was tripled by ammonia and urea treatment. Dry matter digestibility in vivo and in vitro was enhanced by ammonia and urea treatment, and the effect was higher for ammonia treatment. Digestibilities of cell wall constituents were higher (P<.05) for ammonia treated straw, compared to urea-treated straw.
Urinary N excretion was considerably lower (P<.01) and N retention was higher (P<.01) for lambs fed treated straws, compared to those fed urea at feeding time. Ruminal NH3-N and blood urea concentrations were higher (P<.01) for the lambs fed urea at feeding time, compared to lambs fed the others diets.M.S.
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URIAS, ALEJANDRO RUBEN. "EFFECT OF DIETARY CONCENTRATE LEVELS ON IN SITU DRY MATTER DISAPPEARANCE, NEUTRAL DETERGENT FIBER DISAPPEARANCE AND DIGESTION KINETICS OF ALFALFA HAY, WHEAT STRAW AND STEAM PROCESSED AND FLAKED MILO GRAIN". Diss., The University of Arizona, 1986. http://hdl.handle.net/10150/183830.
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A replicated 3 x 3 latin square design (3 periods and 6 rumen fistulated steers) was utilized to investigate the effect of dietary concentrate levels (30, 60 and 90%) on the in situ disappearance of dry matter (ISDMD) and neutral detergent fiber (NDF) and kinetics of fiber digestion of alfalfa hay, wheat straw and steam processed and flaked milo (SPFM). Concentrate levels of 30 and 60% did not affect (P > .05) ISDMD or NDF disappearance from any of the substrates. However, ISDMD and NDF disappearance of all substrates were depressed (P < .05) by the 90% concentrate diet. The degradation of the potentially digestible fiber in these substrates followed first order kinetics at all concentrate levels. Fiber digestion kinetics were not affected (P > .05) by dietary concentrate levels of 30 or 60%. Incubation of substrates in animals receiving the 90% concentrate diet resulted in lower potential extent of digestion (P > .05) and decreased rate of degradation (P > .05) for all substrates. However, digestion was not complete even after incubations of 168-h, and thus, it is possible that the potential extent of digestion was underestimated. For all substrates lag time of digestion appeared to increase in the 90% concentrate diet. However, influx of NDF-like material into the bag during incubation makes the validity of the lag times obtained in situ questionable. Ruminal pH was depressed in animals consuming the 90% concentrate diet and could be at least one of the factors responsible for the decreased (P > .05) fiber digestibility in this diet. Mean ruminal pH of 6.5 (30 and 60% concentrate diets) did not depress (P > .05) fiber digestibility while a mean pH of 6.2 resulted in a lower (P > .05) fiber digestibility in the rumen.
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Ilse, Breanne Rose. "Effects of Linseed Meal on Growth and Reproductive Performance in Ruminants". Thesis, North Dakota State University, 2011. https://hdl.handle.net/10365/28744.
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Linseed meal (LSM) was fed to ovariectomized ewes exposed to estradiol 17-? (E2)
implants over time, and LSM was supplemented to beef cows during late gestation and
early lactation to evaluate the estrogenic potential of the phytoestrogen secoisolariciresinol
diglycoside (SDG) found in LSM. Forty-eight ovariectomized ewes were fed a diet
containing 12.5% LSM for 0, 1, 7, or 14 d and implanted with estradiol-17? (E2) for 0, 6,
or 24 h before tissue collection. Uterine cellular proliferation, vascularity, and the
expression of the angiogenic factors and their receptors were recorded. There was an
interaction of LSM and E2 on uterine mass (P = 0.05). At 24 h of E2 exposure,
proliferation was reduced (P < 0.001) when ewes were fed for 14 d compared to being fed
0 or 1 days. There was a LSM x E2 interaction (P ? 0.03) on VEGF receptor-2 (KDR) and
hasicfihrohlast growth factor receptor (FGFR2) decreasing by 24 h E2 exposure.
Exposure of LSM and E2 may impact the estrogenic response of sensitive tissue.
Two studies were conducted to examine the effects of supplementing beef cows
with LSM during late gestation or early lactation on calf growth and development. In
Experiment 1, multiparous cows received either LSM or a control (CON) supplement
(approximately 10% diet dry matter) for the last 60 d of gestation. Offspring weights,
ADG, heifer age at puberty, conception percentage and steer carcass characteristics were
recorded. In Experiment 2, multiparous cows received LSM or CON supplements
(approximately 10% diet dry matter) during the first 60 d of lactation, and only heifer calf
development performance was observed and recorded. Heifer calf weight, average daily
gain, and attainment of puberty were assessed. For both experiments, birth weight, weaning
weight and ADG were not affected (P > 0.31) by LSM supplementation. While final body
weights were heavier (P = 0.04) for steer calves in Experiment 1, there was no effect (P =
0.09) of supplementation on carcass characteristics. In Experiment 1 and 2, attainment of
puberty in heifer calves was not influenced (P > 0.58) by supplement type. Linseed meal
supplementation during late gestation or early lactation does not appear to have a negative
impact on calf growth, onset of puberty in heifer calves, or steer carcass quality,
implicating that it may make a good supplement choice in cattle.
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Mohamed, Neijat. "Assessing the feeding value of pea straw and evaluating biological methods to improve its utilisation by ruminants". Title page, contents and abstract only, 1995. http://web4.library.adelaide.edu.au/theses/09AS/09asm697.pdf.
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Copies of the author's previously published articles inserted. Bibliography: leaves 142-159. Provides information on the significance of pea straw as a ruminant feed and the potential of two biological techniques for improving its feeding value.
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Yulistiani, Dwi. "The nutritive value of rice straw varieties for ruminants". Title page, Contents and Abstract only, 1997. http://web4.library.adelaide.edu.au/theses/09AS/09asy95.pdf.
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Bibliography: leaves 102-114. This study evaluates the difference in the nutritive value of rice straw varieties using chemical composition and digestibility measurements. It assesses several methods for improving the nutritive value of rice straw and the effect of urea treatment and rice straw quality on the colonisation of ruminal fungi and the characteristics of stem tissue structure.
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Mukisira, Ephraim Amiani. "The influence of alkaloids on voluntary intake and performance by ruminants fed diets containing lupin seed in Kenya". Thesis, McGill University, 1994. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=41730.
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Lupin seed has the potential to be used as a protein supplement in ruminant rations, but its usage is restricted by the presence of alkaloids. Experiments were conducted with the aim of determining whether the removal of specific toxic alkaloids, lupanine and 13-hydroxylupanine, from crushed lupin seed (CLS) would improve the organic matter intake (OMI) and average daily gains (ADG) in lambs, and also improve the lactation performance in dairy cows. Effects on liver function in lambs were also investigated. An additional study on the effects of the detoxification of CLS on the degradation of protein was conducted. In Experiment 1, 30 growing Corriedale lambs were fed five diets for 105 d according to a randomized complete block design (RCBD) with six blocks. Two diets contained intact CLS at 15% (LUI-15) or 30% (LUI-30) of DM; two other diets contained detoxified CLS at 15% (LUD-15) or 30% (LUD-30) and the control (CON) diet was supplemented with crushed sunflower seed, which was locally prepared, on-farm. All diets were formulated to be isonitrogenous (16% CP). The alkaloid content (lupanine and 13-OH lupanine) of the diets was.31,.70,.21 and.52% for LUI-15, LUI-30, LUD-15 and LUD-30, respectively. Thus, diets containing detoxified CLS contained approximately 30% less alkaloids than those with intact CLS. Estimates of ADG of lambs fed diets containing detoxified CLS was higher (P $<$.01) (120 g.d$ sp{-1}$) than that of lambs fed intact CLS (76 g.d$ sp{-1}$). The OMI (73 g/Wkg$ sp{0.75}$) was also higher, although not significantly, for lambs fed diets containing detoxified CLS. Feed conversion efficiency was increased (P $<$.05) by the detoxification of CLS. Lambs fed diets containing intact CLS consumed more OMI as time progressed, suggesting that adaptation to CLS might have occurred. Lambs fed diets containing intact CLS demonstrated increased trends in the activity in plasma of the enzymes glutamic oxaloacetic transferase (GOT) and $ gamma$-glutamyl transfer
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Silva, Tâmara Lúcia dos Santos [UNESP]. "Qualidade da carne de cordeiros alimentados com levedura seca inativa (saccharomyces cerevisiae) da cana-de-açúcar em substituição ao farelo de soja". Universidade Estadual Paulista (UNESP), 2012. http://hdl.handle.net/11449/105098.
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Universidade Estadual Paulista (UNESP)
O objetivo do presente estudo foi avaliar a qualidade da carne de cordeiros mestiços Santa Inês alimentados com levedura (Saccharomyces cerevisiae) inativa da cana-de-açúcar em substituição ao farelo de soja. Foram ofertadas dietas com quatro níveis de substituição (0, 33, 66 e 100%) de levedura aos animais que foram abatidos com 35 kg de peso vivo. Foram realizadas as análises físicas e a determinação de umidade, proteínas, cinzas, gordura e colesterol no músculo Longissimus dorsi. Os níveis de substituição de 33 e 100% influenciaram a composição protéica da carne e os valores de lipídio não apresentaram diferença entre os tratamentos (P>0,05). O teor de colesterol foi menor para os animais que receberam dietas contendo levedura. O índice de fragmentação miofibrilar apresentou diferença entre os níveis de substituição. A maciez, quando analisado pelo método físico, foi satisfatória em carnes do tratamento 33% de substituição
This study aimed to evaluate Santa Inês lambs meat wich was fed with inactive yeast (Saccharomyces cerevisiae) instead of soy meal. The treatments offer were four levels (0, 33, 66 e 100%) of yeast to animals until the weight average 35 kg and they were slaughtered. The analyses were physical and humidity determination, protein, ash, fat cholesterol and in Longissimus dorsi muscle. The inclusion levels influenced in the protein composition of meat. The lipid values were similar (p> 0,05) between the treatments. The values of the tenderness fragmentation index showed variation between treatments. When the softness was analyzed by a physical method it confirmed that the meat has a satisfactory level of tenderness and cholesterol is similar to animals fed with yeast
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Silva, Tâmara Lúcia dos Santos 1984. "Qualidade da carne de cordeiros alimentados com levedura seca inativa (saccharomyces cerevisiae) da cana-de-açúcar em substituição ao farelo de soja /". Botucatu : [s.n.], 2012. http://hdl.handle.net/11449/105098.
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Orientador: André Mendes JorgeCoorientador: José Teodorico de Araújo Filho
Banca: Cledson Augusto Garcia
Banca: Paulo Roberto Meirelles
Banca: Cristiana Andrighetto
Banca: Janaina Haddich Conte
Resumo: O objetivo do presente estudo foi avaliar a qualidade da carne de cordeiros mestiços Santa Inês alimentados com levedura (Saccharomyces cerevisiae) inativa da cana-de-açúcar em substituição ao farelo de soja. Foram ofertadas dietas com quatro níveis de substituição (0, 33, 66 e 100%) de levedura aos animais que foram abatidos com 35 kg de peso vivo. Foram realizadas as análises físicas e a determinação de umidade, proteínas, cinzas, gordura e colesterol no músculo Longissimus dorsi. Os níveis de substituição de 33 e 100% influenciaram a composição protéica da carne e os valores de lipídio não apresentaram diferença entre os tratamentos (P>0,05). O teor de colesterol foi menor para os animais que receberam dietas contendo levedura. O índice de fragmentação miofibrilar apresentou diferença entre os níveis de substituição. A maciez, quando analisado pelo método físico, foi satisfatória em carnes do tratamento 33% de substituição
Abstract : This study aimed to evaluate Santa Inês lambs meat wich was fed with inactive yeast (Saccharomyces cerevisiae) instead of soy meal. The treatments offer were four levels (0, 33, 66 e 100%) of yeast to animals until the weight average 35 kg and they were slaughtered. The analyses were physical and humidity determination, protein, ash, fat cholesterol and in Longissimus dorsi muscle. The inclusion levels influenced in the protein composition of meat. The lipid values were similar (p> 0,05) between the treatments. The values of the tenderness fragmentation index showed variation between treatments. When the softness was analyzed by a physical method it confirmed that the meat has a satisfactory level of tenderness and cholesterol is similar to animals fed with yeast
Doutor
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McEleney, E. "Individual feed intake by ruminants in group feeding situations". Thesis, University of Glasgow, 1985. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.374546.
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Goosen, Liezel. "The effect of an exogenous fibrolytic enzyme on forage digestibility parameters". Thesis, Stellenbosch : Stellenbosch University, 2004. http://hdl.handle.net/10019.1/50141.
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Thesis (MScAgric) -- Stellenbosch University, 2004.ENGLISH ABSTRACT: The ruminant has the ability to utilize forages more efficiently than any other production animal. The utilization of forage fibre is an important aspect of ruminant production systems, as this is the main source of energy available to the animal. The availability of high-fibre forage nutrients is, however, restricted by cell wall degradability, and since low quality forages contribute a great deal to ruminant production systems worldwide, the improvement of this degradation process is of major economic importance. The use of exogenous fibre degrading enzymes has been proposed as a means of enhancing this process, with positive results being obtained from in vitro studies incorporating exogenous enzyme preparations. Positive in vivo results with regard to forage digestibility and other animal production parameters have consequently also been obtained following the addition of exogenous fibre-degrading enzyme preparations to the ruminant diet. Two initial screening experiments were undertaken in order to identify fungal enzyme preparations that may have a positive effect on in vitro fibre degradability. The initial screening employed an in vitro organic matter digestibility technique, and was successful in identifying at least six enzyme preparations displaying enhanced digestibility results that were statistically significant. A second in vitro gas production procedure was used to confirm results obtained from organic matter digestibility assays, as well as to increase screening capacity in order to evaluate new enzyme preparations more time-efficiently. Statistical analysis of results obtained from the secondary screening identified various enzyme candidates producing promising results. Only one of these, Abo 374, proved to be statistically superior to the control and other enzyme preparations. A growth trial was subsequently conducted to assess the performance of this enzyme in vivo. The trial involved individual feeding of 32 Dohne Merino ram lambs grouped according to weights into four groups consisting of 8 lambs each. Each group represented a specific application level of enzyme to the wheat straw component of a high fibre diet, amounting to 10, 5, or 1 ml enzyme supematant/kg straw. The enzyme was diluted with water at appropriate rates to obtain an application rate of 300ml/kg straw. The fourth (control) group was treated with water at the same application rate. The trial was conducted over a period of six weeks, during which feed intakes, weekly weight gains, as well as feed conversion efficiencies were recorded. Results suggested significant weight gains in the high (10ml/kg) and medium (5ml/kg) treatment groups, indicated by a P-value of 0.04. Similarly, feed conversion efficiencies were improved for above-mentioned groups (P=0.05), while feed intakes did not differ significantly between the four experimental groups.
AFRIKAANSE OPSOMMING: Die herkouer besit die vermoee om ruvoere beter as enige ander produksiedier te kan benut. Die gebruik van ruvoervesel is 'n belangrike aspek van herkouer produksiesisteme, aangesien ruvoere die hoof bron van energie aan die herkouer verskaf. Die beskikbaarheid van hoe-vesel ruvoer nutriente word egter beperk deur die degradeerbaarheid van die selwand, en aangesien lae kwaliteit ruvoere 'n groot bydrae tot wereldwye herkouer-produksiesisteme maak, is die moontlike verbetering van hierdie degraderingsproses van groot ekonomiese belang. In 'n poging om hierdie verteringsproses te help bevoordeel, is die gebruik van eksogene veselverterende ensieme ondersoek, en positiewe resultate is verkry wanneer hierdie ensieme in in vitro studies gebruik is. Goeie verbeterings ten opsigte van ruvoer verteerbaarheid en ander diereproduksie parameters is ook verkry deur middel van in vivo studies waar eksogene ensieme by die ruvoer van herkouers gevoeg IS. Twee eksperimente is ondemeem in 'n poging om ensiempreparate wat 'n moontlike positiewe effek op in vitro veselvertering mag he, te identifiseer. Die eerste, 'n in vitro organiese materiaal verteerbaarheid tegniek, was suksesvol in die identifisering van minstens ses ensiem preparate wat statisties betekenisvolle verbeterings ten opsigte van verteringsresultate geproduseer het. 'n Tweede in vitro gasproduksie prosedure is vervolgens gebruik om resultate verkry vanaf die eerste tegniek, te bevestig, asook om evalueringskapasiteit te vergroot en sodoende, nuwe ensiempreparate meer tydseffektief te evalueer. Statistiese evaluering van resultate verkry uit die tweede in vitro tegniek het 'n reeks ensieme met positiewe resultate opgelewer. Een van hierdie, Ab0374, het statisties betekenisvolle resultate ten opsigte van die kontrole, sowel as ander ensieme getoon. In 'n volgende eksperiment is 'n groeiproef gedoen om die effektiwiteit van hierdie ensiem in vivo te toets. In die proef is 32 Dohne Merino ramlammers op grond van hul gewig in vier groepe van agt skape elk verdeel, en individueel gevoer. Die groepe het verskillende toedieningsvlakke van die toetsensiem, toegedien tot die koringstrooi komponent van 'n hoe-vesel dieet, ontvang. Toedieningsvlakke was 10, 5, of lml ensiemkonsentraat/kg strooi. Elke groep se ensiemkonsentraat is verdun met die toepaslike hoeveelheid water om 'n toedieningsvlak van 300ml ensiemoplossing/kg koringstrooi te verkry. 'n Vierde groep is behandel slegs met water teen dieselfde toedieningsvlak, en het gedien as 'n kontrole. Die eksperiment is oor 'n periode van 6 weke uitgevoer. Tydens die proeftydperk is voerinnames, weeklikse gewigstoenames, sowel as voeromsetverhoudings, gedokumenteer. Resultate het betekenisvolle gewigstoenames in die hoe (lOml/kg) en medium (5ml/kg) groepe opgelewer, aangedui deur 'n P-waarde van 0.04. Voeromsetverhoudinge het ook verbeteringe getoon vir bogenoemde twee groepe (P=0.05), terwyl voerinnames nie merkbaar tussen die vier groepe verskil het nie.
Livros sobre o assunto "Ruminants Feeding and feeds"
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Ørskov, E. R. Energy nutrition in ruminants. London: Elsevier, 1990.
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M, Chenost, Reiniger P, European Cooperation in the Field of Scientific and Technical Research (Organization), Commission of the European Communities. e Institut national de la recherche agronomique (France), eds. Evaluation of straws in ruminant feeding. London: Elsevier Applied Science, 1989.
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Coyle, John E. In vivo digestibility studies of ruminant feed ingredients. Dublin: University College Dublin, 1996.
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Preston, T. R. Matching ruminant production systems with available resources in the tropics and sub-tropics. Armidale: Penambul Books, 1987.
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Müller, Z. O. Fattening of ruminants under feedlot system in Pakistan. Islamabad: Pakistan Agricultural Research Council, 1986.
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Cronin, Edward. The in vivo digestibility of ruminant seed ingredients. Dublin: University College Dublin, 1996.
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Krishnamoorthy, Uppoor. Rearing young ruminants on milk replacers and starter feeds. Rome: Food and Agriculture Organization of the United Nations, 2011.
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Ørskov, E. R. The feeding of ruminants: Principles and practice. Marlow: Chalcombe, 1987.
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The feeding of ruminants: Principles and practice. 2a ed. Welton: Chalcombe, 1998.
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Tillman, Allen D. A guide to the feeding and nutrition of ruminants in the tropics. Morrilton, AR: Winrock International Institute for Agricultural Development, 1986.
Encontre o texto completo da fonteCapítulos de livros sobre o assunto "Ruminants Feeding and feeds"
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Hawkey, Kerensa, John Brameld, Tim Parr, Andrew Salter e Heidi Hall. "Suitability of insects for animal feeding." In Insects as animal feed: novel ingredients for use in pet, aquaculture and livestock diets, 26–38. Wallingford: CABI, 2021. http://dx.doi.org/10.1079/9781789245929.0004.
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Abstract This chapter explores the nutritional composition of insects and the potential benefits and drawbacks for their inclusion into pet, poultry, pig, ruminant and aquatic animal feeds. Six species are most commonly described for use in animal feed, namely black soldier fly (BSF; Hermetia illucens) larvae and prepupae, yellow mealworm (YM; Tenebrio molitor) larvae, superworm (SW; Zophobas morio) larvae, lesser mealworm (LM; Alphitobius diaperinus) larvae, house fly (HF; Musca domestica) larvae and house crickets (HC; Acheta domesticus). The nutritional composition, variability observed due to manipulation of feed source, age, developmental stage and suitability for inclusion into animal feed are compared.
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Tesfa, Kal’ab N., e Fithawi Mehari. "Comparative Feeding Value of Halophyte as Alternative Animal Feed for Small Ruminants in Eritrea". In Sustainable Agricultural Development, 253–60. Dordrecht: Springer Netherlands, 2011. http://dx.doi.org/10.1007/978-94-007-0519-7_19.
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Lukefahr, Steven. "Feeds and feeding." In Rabbit production, 107–24. 10a ed. Wallingford: CABI, 2022. http://dx.doi.org/10.1079/9781789249811.0008.
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Abstract This chapter discusses the nutrient requirements; feed formulation; feed composition of clovers, grasses, lucerne, concentrates, grains, feed supplements and feed additives; feed processing and feeding of rabbits.
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Tucker, Craig S., e Edwin H. Robinson. "Feeds and Feeding Practices". In Channel Catfish Farming Handbook, 291–315. Boston, MA: Springer US, 1990. http://dx.doi.org/10.1007/978-1-4757-1376-3_11.
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Dryden, Gordon McL. "Digestion of feeds." In Fundamentals of applied animal nutrition, 19–34. Wallingford: CABI, 2021. http://dx.doi.org/10.1079/9781786394453.0003.
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Abstract In this chapter the feeding behaviour and digestive physiology, digestive tract function-intrinsic and microbial digestion; functions and anatomy of the digestive tract in animals are discussed.
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Bustos, D. P. Jaramillo, W. B. Valderrama e A. J. Trujillo Mesa. "5. Small ruminants feeding and effect on cheese composition". In Human Health Handbooks, 73–86. The Netherlands: Wageningen Academic Publishers, 2013. http://dx.doi.org/10.3920/978-90-8686-766-0_5.
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Boni, Zofia. "“My mum feeds me, but really, I eat whatever I want!”". In Feeding Children Inside and Outside the Home, 107–23. Abingdon, Oxon ; New York, NY : Routledge, 2019.: Routledge, 2018. http://dx.doi.org/10.4324/9781315206974-7.
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Nefzaoui, A., H. Ben Salem e M. El Mourid. "Innovations in small ruminants feeding systems in arid Mediterranean areas". In New trends for innovation in the Mediterranean animal production, 99–116. Wageningen: Wageningen Academic Publishers, 2012. http://dx.doi.org/10.3920/978-90-8686-726-4_14.
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Mpairwe, Denis, e David Mutetikka. "Improved feeding for dairy cattle and poultry in smallholder crop-livestock systems." In Sustainable agricultural intensification: a handbook for practitioners in East and Southern Africa, 106–18. Wallingford: CABI, 2022. http://dx.doi.org/10.1079/9781800621602.0008.
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Abstract This chapter describes how cropping systems can be used to supplement feeds to increase dairy and poultry yields. It completes the concept of on-farm integration of crop, livestock and natural resources technologies necessary for sustainable intensification on smallholder farms. The different processes that utilize different feed sources to produce better-quality feed rations for dairy cows and poultry are also discussed.
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Vohnout, K., e C. Jimenez. "Supplemental By-Product Feeds in Pasture-Livestock Feeding Systems in the Tropics". In Tropical Forages in Livestock Production Systems, 71–82. Madison, WI, USA: American Society of Agronomy, Crop Science Society of America, and Soil Science Society of America, 2015. http://dx.doi.org/10.2134/asaspecpub24.c6.
Texto completo da fonteTrabalhos de conferências sobre o assunto "Ruminants Feeding and feeds"
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Matovu, Jacob, e Ahmet Alçiçek. "Investigations and Concerns about the Fate of Transgenic DNA and Protein in Livestock". In International Students Science Congress. Izmir International Guest Student Association, 2021. http://dx.doi.org/10.52460/issc.2021.011.
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The fate of transgenic DNA (tDNA) and protein from feed derived from Genetically Modified organisms (GMOs) in animals has been a major issue since their commercialization in 1996. Several studies have investigated the risks of horizontal gene transfer (HGT) of tDNA and protein to bacteria or animal cells/tissues, but some of the reported data are controversial. Previous reports showed that tDNA fragments or proteins derived from GM plants could not be detected in tissues, fluids, or edible products from livestock. Other researchers have shown that there is a possibility of small fragments entering animal tissues, fluids and organs. This motivated us to update our knowledge about these concerns. Therefore, this review aimed to evaluate the probable transfer and accumulation of tDNA/proteins from transgenic feeds in animal samples (ruminant and non-ruminant) by evaluating the available experimental studies published scientifically. This study found that the tDNA/protein is not completely degraded during feed processing and digestion in Gastro-Intestinal Tract (GIT). In large ruminants (cattle), tDNA fragments/proteins were detected in GIT digesta, rumen fluid, and faeces. In small ruminants (goats), traces of tDNA/proteins were detected in GIT digesta, blood, milk, liver, kidney, heart and muscle. In pigs, they were detected in blood, spleen, liver, kidney, and GIT digesta. In poultry, traces were detected in blood, liver and GIT digesta but not in meat and eggs. Notwithstanding some studies that have shown transfer of tDNA/protein fragments in animal samples, we cannot rely on these few studies to give general evidence for transfer into tissues/fluids and organs of farm animals. However, this study clearly shows that transfer is possible. Therefore, intensive and authentic research should be conducted on GM plants before they are approved for commercial use, investigating issues such as the fate of tDNA or proteins and the effects of feeding GM feed to livestock.
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Ünlü, Elif Işılay, e Ahmet Çınar. "Lesion Detection on Skin Images Using Improved U-Net". In International Students Science Congress. Izmir International Guest Student Association, 2021. http://dx.doi.org/10.52460/issc.2021.022.
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The fate of transgenic DNA (tDNA) and protein of feeds from Genetically Modified organisms (GMOs) in animals has been an important topic since their commercialization in 1996. Several studies have investigated about risks of horizontal gene transfer (HGT) of tDNA and proteins to bacteria or animal cells/tissues, however, the reported data is at times controversial. Earlier reports showed that tDNA fragments or protein derived from GM plants have not been detected in tissues, fluids, or edible products of farm animals. Other researchers have come out to demonstrate that there is the possibility of small fragments leaking out into the animal tissues, fluids and organs. This motivated us to update our knowledge about these concerns. Therefore, this review aimed at assessing the likely transfer and accumulation of tDNA/ proteins from transgenic feeds to animal (ruminants and non-ruminants) samples through evaluating the available experimental scientific published studies. This study has found out that the tDNA or protein is not completely degraded during feed processing and digestion in the Gastro-Intestinal Tract (GIT). In large ruminants (Cattle), tDNA fragments/protein have been detected in the GIT digesta, ruminal fluid and feces. In small ruminants (Goats), traces of tDNA/proteins have been detected in the GIT digesta, blood, milk, liver, kidney, heart and muscle. In pigs, they have been detected in blood, spleen, liver kidney and in the GIT digesta. In poultry, traces have been seen in blood, liver and GIT digesta but not in meat and Eggs. Regardless of some studies that have shown the transfer of tDNA/protein fragments to animal samples, we cannot base on these few studies to give a piece of general evidence about their transfer into tissues/fluids and organs of livestock animals. However, this study clearly shows possible transfer, hence intensive and authentic research on GM crops should be done before they are allowed for commercial use, studying issues like the fate of tDNA or proteins and the effect of feeding GM feeds to livestock.
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ISMAILOV, Ismail Sagidovich, Nina Vladimirovna TREGUBOVA, Rashid Hasanbievich KOCHKAROV, Anna Victorovna MORGUNOVA e Natalija Alecseevna DRIZHD. "THE RELATIONSHEEP OF AMINO ACID METABOLISM WITH PRODUCTIVITY OF GROWING YOUNG SHEEP". In RURAL DEVELOPMENT. Aleksandras Stulginskis University, 2018. http://dx.doi.org/10.15544/rd.2017.125.
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A number of studies on digestion of the ruminants have shown the process of synthesis of all the essential amino acids in the rumen (Abu Fadel, 2004; Trukhachev, V., Zlidnev, N. 2008). However, according to some researches, microbial protein is deficient in methionine and lysine. This assumption also proves the need for further study of the influence of balanced amino acids to these diets on the growth, development and productive performance of the ruminants. Scientific approbation of the issues related to establishing the requirements of young sheep in lysine and methionine with cystine and development of recommendations for their proper balance in animal rations have been conducted along with a series of physiological, scientific and practical experiments. For this purpose, we used the following research methods: preparative, analytical, measuring and calculating. Zootechnical evaluation of young sheep’s diet with different levels of lysine and methionine with cysteine has been conducted, and influence of these components on growth, development, metabolism, the use of amino acids and wool productivity have been studied. The importance of studying the content of amino acids in plasma is evident because they are the indicators of protein metabolism in the animals’ organism and represent themselves the exchange fund when used in the biochemical transformations in the process of updating the protein of the body tissues and the synthesis of animal products. Increase in young sheep’s diet of lysine and methionine can improve their productivity, reduce the cost of feed energy and improve biochemical indexes of meat, taking into account the optimization of its amino acid composition. Efficiency increase of young sheep during the process of the individual development is linked to conditions of feeding and in particular the usefulness of a protein food, which is primarily determined by sufficient intake of essential amino acids - lysine and methionine with cystine. Thus, the inclusion in the diet of growing young sheep some amount of synthetic amino acids – 6–8 grams of lysine and 3–4 g of methionine per 1 fodder unit, enhances oxidation-reduction processes, increase productivity, i.e. wool yield and other indicators. The content of free amino acids in plasma directly depends on their availability in feed. The use of synthetic amino acids (lysine, methionine and cystine) contributed to the increase of their content in a free state in the blood serum, indicating their increased demand in sheep. With the use of different doses of synthetic amino acids (lysine, methionine, cystine) in the diets of young sheep it has been found that the organization of optimum conditions of feeding, care and management of sheep in winter season help to avoid abrupt changes in the structure of the skin, to ensure the normal development of the histological structure and morphogenetic processes.)
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Ghorbel, Roukaya, e Nedim Koşum. "Hydroponic Fodder Production: An Alternative Solution for Feed Scarcity". In 6th International Students Science Congress. Izmir International Guest Student Association, 2022. http://dx.doi.org/10.52460/issc.2022.005.
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Feeds and animal nutrition presents a major sector in providing food security. However, there is a large gap between fodder supply and demand [1]. This gap can be attributed basically to climatic changes, urbanization and increase in meat demand. Nowadays, especially after the covid-19 pandemic, there is a crucial problem in supplying fresh green feed to remote and urban regions. Hydroponic fodder is an alternative solution to provide the sustainability of quality forage for ruminant. Hydroponics can include several crops such as maize, wheat and barley, the fodder can be produced in a short duration (6-10 days) and all year around. The hydroponics fodder present various benefits for animal health. Feeding hydroponically produced fodder increases the digestibility of the nutrients in the ration, which could increase milk production [2]. Hydroponic fodder production is an economic solution particularly where the conventional production of green fodder is limited or unavailable. This technology put forward a solution to address the shortage of forage production caused by the scarcity of green feed in dry seasons and urban areas [3]. That´s why, further studies and academic efforts are needed to expand hydroponic fodder production. The purpose of this study is to review the actual literature on hydroponic fodder production process, nutritious value and effects on livestock production and productivity.
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Cornale, P., A. Mimosi e L. M. Battaglini. "43. Reducing feed-food competition: impact of by-products and grazing in ruminant feeding". In EurSafe 2022. The Netherlands: Wageningen Academic Publishers, 2022. http://dx.doi.org/10.3920/978-90-8686-939-8_43.
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Carrierfenster, Kristen, Christine Rue-Donlon e Rubia Khalak. "Getting to Full Feeds Faster in the Nicu Utilizing Cue-based Feeding". In Selection of Abstracts From NCE 2016. American Academy of Pediatrics, 2018. http://dx.doi.org/10.1542/peds.141.1_meetingabstract.522.
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Đorđevic, Nenad, Dušica Radonjic, Goran Grubic, Bojan Stojanovic, Aleksa Božičkovic e Blagoje Stojkovic. "UTICAJ MASTI OBROKA NA SADRŽAJ ESENCIJALNIH MASNIH KISELINA U MLEČNOJ MASTI PREŽIVARA". In SAVETOVANJE o biotehnologiji sa međunarodnim učešćem. University of Kragujevac, Faculty of Agronomy, 2021. http://dx.doi.org/10.46793/sbt26.219dj.
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In this paper is shown an overview of researches on the effect of nutrition with diets and feeds rich in lipids, on the yield and chemical composition of milk, as well as on the content of polyunsaturated fatty acids (PUFA) in milk fat at ruminants. The numerous studies have shown that many genetic and nongenetic factors affect on the milk fat and fatty acids content variations, whereas the main factor is animal nutrition. In addition to fresh forage intake (on pasture or in the stable) the second most important way to increase the PUFA content is using some oilseeds in rations. Among the researched species (soybean, sunflower, flax, cotton, peanuts), the best results were obtained with using heat-treated soybean, which also provides a higher content of undegradable protein.
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Musial, Abigail, Owen Densel, Conor Merritt, Lacey Yeager, Angela Younts, Jodi Kelley, James Rudloff, Lori Herbst, Angela Statile e Amanda Schondelmeyer. "1 Decreasing time to goal enteral feeds in children with medical complexity with feeding intolerance". In IHI Scientific Symposium. British Medical Journal Publishing Group, 2020. http://dx.doi.org/10.1136/bmjoq-2020-ihi.1.
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Сорокин, Александр, Alexander Sorokin, Валентина Руцкая e Valentina Ruckaya. "LUPIN AS THE BASE FOR PROTEIN COMPONENT OF FEED MIX FOR POULTRY". In Multifunctional adaptive feed production. ru: Federal Williams Research Center of Forage Production and Agroecology, 2019. http://dx.doi.org/10.33814/mak-2019-21-69-110-115.
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The article presents the data of domestic and foreign researchers for feeding of poultry used lupin and its processed products. It’s advisable to use energy-and-protein concentrates made of legumes and cereal grain mixtures to prepare complete feeds. Lupin is one of the main sources of plant protein with unique amino acid composition. In opinion of the majority of researchers lupin containing feeds made according to technic guidance support productivity increasing of poultry growing and improve the quality of the products.
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SKVORCOVA, Lyudmila, e Natal'ya ChURSINA. "Influence of the level of electrolytes in the diets of young quails on their productivity". In Multifunctional adaptive fodder production 29 (77). ru: Federal Williams Research Center of Forage Production and Agroecology, 2022. http://dx.doi.org/10.33814/mak-2022-29-77-196-200.
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Increasing or decreasing the sodium level relative to the control does not adversely affect the level of safety of the livestock and the appetite of the birds. At the same time, feeding quails with compound feeds with a sodium level of 0.4 and 0.6% reduces feed costs per unit of production.
Relatórios de organizações sobre o assunto "Ruminants Feeding and feeds"
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Weinberg, Zwi G., Adegbola Adesogan, Itzhak Mizrahi, Shlomo Sela, Kwnag Jeong e Diwakar Vyas. effect of selected lactic acid bacteria on the microbial composition and on the survival of pathogens in the rumen in context with their probiotic effects on ruminants. United States Department of Agriculture, janeiro de 2014. http://dx.doi.org/10.32747/2014.7598162.bard.
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This research project was performed in context of the apparent probiotic effect of selected lactic acid bacteria (LAB) silage inoculants on the performance of ruminants (improved feed intake, faster live-weight gain, higher milk yields and improved feed efficiency). The overall objective was to find out how LAB affect ruminant performance. The project included several “chapters” as follows: 1. The effect of LAB silage inoculants on the survival of detrimental bacteria in rumen fluid, in vitro study (Weinberg et al., The Volcani Center). An in vitro model was developed to study the interaction between selected LAB and an E. coli strain tagged with green fluorescence protein (GFP) in buffered RF. Results indicated that both LAB inoculants and E. coli survived in the RF for several days; both LAB inoculants and LAB-treated silages did not affect survival of E. coli in rumen fluid in vitro. The effect of feeding baled wheat silages treated with or without three selected LAB silage inoculants on the performance of high-lactating cows (Weinberg et al., The Volcani Center). Treatments included control (no additive), Lacobacillusbuchneri40788 (LB), Lactobacillus plantarumMTD1 40027 (LP) and Pediococcuspentosaceus30168 (PP), each applied at 10⁶ cfu/g FM. The silages were included in the TMR of 32 high milking Holstein cows in a controlled feeding experiment. All baled silages were of good quality. The LB silage had the numerically highest acetic acid and were the most stable upon aerobic exposure. The cows fed the LB silages had the highest daily milk yields, percent milk fat and protein. The microbiome of baled wheat silages and changes during ensiling of wheat and corn (Sela et al., The Volcani Center). Bacterial community of the baled silages was dominated mainly of two genera in total, dominated by Lactobacillus and Clostridium_sensu_stricto_12 with 300 other genera at very low abundance. Fungal community was composed mainly of two genera in total, dominated by Candida and Monascuswith 20 other genera at very low abundance. In addition, changes in the microbiome during ensiling of wheat and corn with and without addition of L. plantarumMTD1 was studied in mini-silos. Overall 236 bacterial genera were identified in the fresh corn but after 3 months Lactobacillus outnumbered all other species by acquiring 95% of relative abundance. The wheat silage samples are still under analysis. The effect of applying LAB inoculants at ensiling on survival of E. coli O157:H7 in alfalfa and corn silages(Adesogan et al., University of Florida). E. coli (10⁵ cfu/g) was applied to fresh alfalfa and corn at ensiling with or without L. plantarumor L. buchneri. The pathogen was added again after about 3 moths at the beginning of an aerobic exposure period. The inoculants resulted in faster decrease in pH as compared with the control (no additives) or E. coli alone and therefore, the pathogen was eliminated faster from these silages. After aerobic exposure the pathogen was not detected in the LAB treated silages, whereas it was still present in the E. coli alone samples. 5. The effect of feeding corn silage treated with or without L. buchnerion shedding of E. coli O157:H7 by dairy cows (Adesogan et al., UFL). BARD Report - Project 4704 Page 2 of 12 Five hundred cows from the dairy herd of the University of Florida were screened for E. coli shedding, out of which 14 low and 13 high shedders were selected. These cows were fed a total mixed ration (TMR) which was inoculated with E. coli O157:H7 for 21 days. The TMR included corn silage treated with or without L. buchneri. The inoculated silages were more stable upon aerobic exposure than the control silages; the silage inoculant had no significant effect on any milk or cow blood parameters. However, the silage inoculant tended to reduce shedding of E. coli regardless of high or low shedders (p = 0.06). 6. The effect of feeding baled wheat silages treated with or without three selected LAB silage inoculants on the rumen microbiome (Mizrahi et al., BGU). Rumen fluid was sampled throughout the feeding experiment in which inoculated wheat silages were included in the rations. Microbial DNA was subsequently purified from each sample and the 16S rRNA was sequenced, thus obtaining an overview of the microbiome and its dynamic changes for each experimental treatment. We observed an increase in OTU richness in the group which received the baled silage inoculated with Lactobacillus Plantarum(LP). In contrast the group fed Lactobacillus buchneri(LB) inoculated silage resulted in a significant decrease in richness. Lower OTU richness was recently associated in lactating cows with higher performance (Ben Shabatet al., 2016). No significant clustering could be observed between the different inoculation treatments and the control in non metric multi-dimentional scaling, suggesting that the effect of the treatments is not the result of an overall modulation of the microbiome composition but possibly the result of more discrete interactions. Significant phylum level changes in composition also indicates that no broad changes in taxa identity and composition occurred under any treatment A more discrete modulation could be observed in the fold change of several taxonomic groups (genus level analysis), unique to each treatment, before and after the treatment. Of particular interest is the LB treated group, in which several taxa significantly decreased in abundance. BARD Report - Project 4704 Page 3 of 12
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Brosh, Arieh, Gordon Carstens, Kristen Johnson, Ariel Shabtay, Joshuah Miron, Yoav Aharoni, Luis Tedeschi e Ilan Halachmi. Enhancing Sustainability of Cattle Production Systems through Discovery of Biomarkers for Feed Efficiency. United States Department of Agriculture, julho de 2011. http://dx.doi.org/10.32747/2011.7592644.bard.
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Feed inputs represent the largest variable cost of producing meat and milk from ruminant animals. Thus, strategies that improve the efficiency of feed utilization are needed to improve the global competitiveness of Israeli and U.S. cattle industries, and mitigate their environmental impact through reductions in nutrient excretions and greenhouse gas emissions. Implementation of innovative technologies that will enhance genetic merit for feed efficiency is arguably one of the most cost-effective strategies to meet future demands for animal-protein foods in an environmentally sustainable manner. While considerable genetic variation in feed efficiency exist within cattle populations, the expense of measuring individual-animal feed intake has precluded implementation of selection programs that target this trait. Residual feed intake (RFI) is a trait that quantifies between-animal variation in feed intake beyond that expected to meet energy requirements for maintenance and production, with efficient animals being those that eat less than expected for a given size and level of production. There remains a critical need to understand the biological drivers for genetic variation in RFI to facilitate development of effective selection programs in the future. Therefore, the aim of this project was to determine the biological basis for phenotypic variation in RFI of growing and lactating cattle, and discover metabolic biomarkers of RFI for early and more cost-effective selection of cattle for feed efficiency. Objectives were to: (1) Characterize the phenotypic relationships between RFI and production traits (growth or lactation), (2) Quantify inter-animal variation in residual HP, (3) Determine if divergent RFIphenotypes differ in HP, residual HP, recovered energy and digestibility, and (4) Determine if divergent RFI phenotypes differ in physical activity, feeding behavior traits, serum hormones and metabolites and hepatic mitochondrial traits. The major research findings from this project to date include: In lactating dairy cattle, substantial phenotypic variation in RFI was demonstrated as cows classified as having low RMEI consumed 17% less MEI than high-RMEI cows despite having similar body size and lactation productivity. Further, between-animal variation in RMEI was found to moderately associated with differences in RHP demonstrating that maintenance energy requirements contribute to observed differences in RFI. Quantifying energetic efficiency of dairy cows using RHP revealed that substantial changes occur as week of lactation advances—thus it will be critical to measure RMEI at a standardized stage of lactation. Finally, to determine RMEI in lactating dairy cows, individual DMI and production data should be collected for a minimum of 6 wk. We demonstrated that a favorably association exists between RFI in growing heifers and efficiency of forage utilization in pregnant cows. Therefore, results indicate that female progeny from parents selected for low RFI during postweaning development will also be efficient as mature females, which has positive implications for both dairy and beef cattle industries. Results from the beef cattle studies further extend our knowledge regarding the biological drivers of phenotypic variation in RFI of growing animals, and demonstrate that significant differences in feeding behavioral patterns, digestibility and heart rate exist between animals with divergent RFI. Feeding behavior traits may be an effective biomarker trait for RFI in beef and dairy cattle. There are differences in mitochondrial acceptor control and respiratory control ratios between calves with divergent RFI suggesting that variation in mitochondrial metabolism may be visible at the genome level. Multiple genes associated with mitochondrial energy processes are altered by RFI phenotype and some of these genes are associated with mitochondrial energy expenditure and major cellular pathways involved in regulation of immune responses and energy metabolism.
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Brosh, Arieh, David Robertshaw, Yoav Aharoni, Zvi Holzer, Mario Gutman e Amichai Arieli. Estimation of Energy Expenditure of Free Living and Growing Domesticated Ruminants by Heart Rate Measurement. United States Department of Agriculture, abril de 2002. http://dx.doi.org/10.32747/2002.7580685.bard.
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Research objectives were: 1) To study the effect of diet energy density, level of exercise, thermal conditions and reproductive state on cardiovascular function as it relates to oxygen (O2) mobilization. 2) To validate the use of heart rate (HR) to predict energy expenditure (EE) of ruminants, by measuring and calculating the energy balance components at different productive and reproductive states. 3) To validate the use of HR to identify changes in the metabolizable energy (ME) and ME intake (MEI) of grazing ruminants. Background: The development of an effective method for the measurement of EE is essential for understanding the management of both grazing and confined feedlot animals. The use of HR as a method of estimating EE in free-ranging large ruminants has been limited by the availability of suitable field monitoring equipment and by the absence of empirical understanding of the relationship between cardiac function and metabolic rate. Recent developments in microelectronics provide a good opportunity to use small HR devices to monitor free-range animals. The estimation of O2 uptake (VO2) of animals from their HR has to be based upon a consistent relationship between HR and VO2. The question as to whether, or to what extent, feeding level, environmental conditions and reproductive state affect such a relationship is still unanswered. Studies on the basic physiology of O2 mobilization (in USA) and field and feedlot-based investigations (in Israel) covered a , variety of conditions in order to investigate the possibilities of using HR to estimate EE. In USA the physiological studies conducted using animals with implanted flow probes, show that: I) although stroke volume decreases during intense exercise, VO2 per one heart beat per kgBW0.75 (O2 Pulse, O2P) actually increases and measurement of EE by HR and constant O2P may underestimate VO2unless the slope of the regression relating to heart rate and VO2 is also determined, 2) alterations in VO2 associated with the level of feeding and the effects of feeding itself have no effect on O2P, 3) both pregnancy and lactation may increase blood volume, especially lactation; but they have no effect on O2P, 4) ambient temperature in the range of 15 to 25°C in the resting animal has no effect on O2P, and 5) severe heat stress, induced by exercise, elevates body temperature to a sufficient extent that 14% of cardiac output may be required to dissipate the heat generated by exercise rather than for O2 transport. However, this is an unusual situation and its affect on EE estimation in a freely grazing animal, especially when heart rate is monitored over several days, is minor. In Israel three experiments were carried out in the hot summer to define changes in O2P attributable to changes in the time of day or In the heat load. The animals used were lambs and young calves in the growing phase and highly yielding dairy cows. In the growing animals the time of day, or the heat load, affected HR and VO2, but had no effect on O2P. On the other hand, the O2P measured in lactating cows was affected by the heat load; this is similar to the finding in the USA study of sheep. Energy balance trials were conducted to compare MEI recovery by the retained energy (RE) and by EE as measured by HR and O2P. The trial hypothesis was that if HR reliably estimated EE, the MEI proportion to (EE+RE) would not be significantly different from 1.0. Beef cows along a year of their reproductive cycle and growing lambs were used. The MEI recoveries of both trials were not significantly different from 1.0, 1.062+0.026 and 0.957+0.024 respectively. The cows' reproductive state did not affect the O2P, which is similar to the finding in the USA study. Pasture ME content and animal variables such as HR, VO2, O2P and EE of cows on grazing and in confinement were measured throughout three years under twenty-nine combinations of herbage quality and cows' reproductive state. In twelve grazing states, individual faecal output (FO) was measured and MEI was calculated. Regression analyses of the EE and RE dependent on MEI were highly significant (P<0.001). The predicted values of EE at zero intake (78 kcal/kgBW0.75), were similar to those estimated by NRC (1984). The EE at maintenance condition of the grazing cows (EE=MEI, 125 kcal/kgBW0.75) which are in the range of 96.1 to 125.5 as presented by NRC (1996 pp 6-7) for beef cows. Average daily HR and EE were significantly increased by lactation, P<0.001 and P<0.02 respectively. Grazing ME significantly increased HR and EE, P<0.001 and P<0.00l respectively. In contradiction to the finding in confined ewes and cows, the O2P of the grazing cows was significantly affected by the combined treatments (P<0.00l ); this effect was significantly related to the diet ME (P<0.00l ) and consequently to the MEI (P<0.03). Grazing significantly increased O2P compared to confinement. So, when EE of grazing animals during a certain season of the year is estimated using the HR method, the O2P must be re measured whenever grazing ME changes. A high correlation (R2>0.96) of group average EE and of HR dependency on MEI was also found in confined cows, which were fed six different diets and in growing lambs on three diets. In conclusion, the studies conducted in USA and in Israel investigated in depth the physiological mechanisms of cardiovascular and O2 mobilization, and went on to investigate a wide variety of ruminant species, ages, reproductive states, diets ME, time of intake and time of day, and compared these variables under grazing and confinement conditions. From these combined studies we can conclude that EE can be determined from HR measurements during several days, multiplied by O2P measured over a short period of time (10-15 min). The study showed that RE could be determined during the growing phase without slaughtering. In the near future the development microelectronic devices will enable wide use of the HR method to determine EE and energy balance. It will open new scopes of physiological and agricultural research with minimizes strain on animals. The method also has a high potential as a tool for herd management.
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Mitchell, Brian G., Amir Neori, Charles Yarish, D. Allen Davis, Tzachi Samocha e Lior Guttman. The use of aquaculture effluents in spray culture for the production of high protein macroalgae for shrimp aqua-feeds. United States Department of Agriculture, janeiro de 2013. http://dx.doi.org/10.32747/2013.7597934.bard.
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The FAO has projected a doubling in world demand for seafood during the 21 ed from aquaculture of marine fish and shrimps fed primarily on fishmeal-based aquafeeds. However, current practices of high intensity monoculture of shrimp in coastal ponds and fish in offshore pens have been strongly criticized as being ecologically and socially unsustainable. This view derives from un- checked eutrophication of coastal marine ecosystems from fish farm effluents, and the destruction of coastal estuarine ecosystems by shrimp farm constructions, plus aquaculture’s reliance on wild-caught small fish - which are excellent food for humans, but instead are rendered into fishmeal and fish oil for formulating aquafeeds. Fishmeal-sparing and waste- reduction aquafeeds can only delay the time when fed aquaculture product are priced out of affordability for most consumers. Additionally, replacement of fishmeal protein and fish oil by terrestrial plant sources such as soybean meal and oil directly raises food costs for human communities in developing nations. New formulations incorporating sustainably-produced marine algal proteins and oils are growing in acceptance as viable and practical alternatives. This BARD collaborative research project investigated a sustainable water-sparing spray/drip culture method for producing high-protein marine macrophyte meals for incorporation into marine shrimp and fish diets. The spray culture work was conducted at laboratory-scale in the USA (UCSD-SIO) using selected Gracilariaand Ulvastrains isolated and supplied by UCONN, and outdoors at pilot-scale in Israel (IOLR-NCM) using local strains of Ulvasp., and nitrogen/phosphorus-enriched fish farm effluent to fertilize the spray cultures and produce seaweed biomass and meals containing up to 27% raw protein (dry weight content). Auburn University (USA) in consultation with TAMUS (USA) used the IOLR meals to formulate diets and conduct marine shrimp feeding trials, which resulted in mixed outcomes, indicating further work was needed to chemically identify and remove anti-nutritional elements present in the IOLR-produced seaweed meals.
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Weinberg, Zwi G., Richard E. Muck, Nathan Gollop, Gilad Ashbell, Paul J. Weimer e Limin Kung, Jr. effect of lactic acid bacteria silage inoculants on the ruminal ecosystem, fiber digestibility and animal performance. United States Department of Agriculture, setembro de 2003. http://dx.doi.org/10.32747/2003.7587222.bard.
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The overall objective of the whole research was to elucidate the mechanisms by which LAB silage inoculants enhance ruminant performance. The results generated will permit the development of better silage inoculants that maximize both silage preservation and animal performance. For this one-year BARD feasibility study, the objectives were to: 1. determine whether lactic acid bacteria (LAB) used in inoculants for silage can survive in rumen fluid (RF) 2.select the inoculants that survived best, and 3. test whether LAB silage inoculants produce bacteriocins-like substances. The most promising strains will be used in the next steps of the research. Silage inoculants containing LAB are used in order to improve forage preservation efficiency. In addition, silage inoculants enhance animal performance in many cases. This includes improvements in feed intake, liveweight gain and milk production in 25-40% of studies reviewed. The cause for the improvement in animal performance is not clear but appears to be other than direct effect of LAB inoculants on silage fermentation. Results from various studies suggest a possible probiotic effect. Our hypothesis is that specific LAB strains interact with rumen microorganisms which results in enhanced rumen functionality and animal performance. The first step of the research is to determine whether LAB of silage inoculants survive in RF. Silage inoculants (12 in the U.S. and 10 in Israel) were added to clarified and strained RF. Inoculation rate was 10 ⁶ (clarified RF), 10⁷ (strained RF) (in the U.S.) and 10⁷, 10⁸ CFU ml⁻¹ in Israel (strained RF). The inoculated RF was incubated for 72 and 96 h at 39°C, with and without 5 g 1⁻¹ glucose. Changes in pH, LAB numbers and fermentation products were monitored throughout the incubation period. The results indicated that LAB silage inoculants can survive in RF. The inoculants with the highest counts after 72 h incubation in rumen fluid were Lactobacillus plantarum MTD1 and a L. plantarum/P. cerevisiae mixture (USA) and Enterococcus faecium strains and Lactobacillus buchneri (Israel). Incubation of rumen fluid with silage LAB inoculants resulted in higher pH values in most cases as compared with that of un-inoculated controls. The magnitude of the effect varied among inoculants and typically was enhanced with the inoculants that survived best. This might suggest the mode of action of LAB silage inoculants in the rumen as higher pH enhances fibrolytic microorganisms in the rumen. Volatile fatty acid (VFA) concentrations in the inoculated RF tended to be lower than in the control RF after incubation. However, L. plalltarull1 MTDI resulted in the highest concentrations of VFA in the RF relative to other inoculants. The implication of this result is not as yet clear. In previous research by others, feeding silages which were inoculated with this strain consistently enhanced animal performance. These finding were recently published in Weinberg et.al.. (2003), J. of Applied Microbiology 94:1066-1071 and in Weinberg et al.. (2003), Applied Biochemistry and Biotechnology (accepted). In addition, some strains in our studies have shown bacteriocins like activity. These included Pediococcus pentosaceus, Enterococcus faecium and Lactobacillus plantarum Mill 1. These results will enable us to continue the research with the LAB strains that survived best in the rumen fluid and have the highest potential to affect the rumen environment.
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Klement, Eyal, Elizabeth Howerth, William C. Wilson, David Stallknecht, Danny Mead, Hagai Yadin, Itamar Lensky e Nadav Galon. Exploration of the Epidemiology of a Newly Emerging Cattle-Epizootic Hemorrhagic Disease Virus in Israel. United States Department of Agriculture, janeiro de 2012. http://dx.doi.org/10.32747/2012.7697118.bard.
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In September 2006 an outbreak of 'Bluetongue like' disease struck the cattle herds in Israel. Over 100 dairy and beef cattle herds were affected. Epizootic hemorrhagic disease virus (EHDV) (an Orbivirusclosely related to bluetongue virus (BTV)), was isolated from samples collected from several herds during the outbreaks. Following are the aims of the study and summary of the results: which up until now were published in 6 articles in peer-reviewed journals. Three more articles are still under preparation: 1. To identify the origin of the virus: The virus identified was fully sequenced and compared with the sequences available in the GenBank. It appeared that while gene segment L2 was clustered with EHDV-7 isolated in Australia, most of the other segments were clustered with EHDV-6 isolates from South-Africa and Bahrain. This may suggest that the strain which affected Israel on 2006 may have been related to similar outbreaks which occurred in north-Africa at the same year and could also be a result of reassortment with an Australian strain (Wilson et al. article in preparation). Analysis of the serological results from Israel demonstrated that cows and calves were similarly positive as opposed to BTV for which seropositivity in cows was significantly higher than in calves. This finding also supports the hypothesis that the 2006 EHD outbreak in Israel was an incursive event and the virus was not present in Israel before this outbreak (Kedmi et al. Veterinary Journal, 2011) 2. To identify the vectors of this virus: In the US, Culicoides sonorensis was found as an efficient vector of EHDV as the virus was transmitted by midges fed on infected white tailed deer (WTD; Odocoileusvirginianus) to susceptible WTD (Ruder et al. Parasites and Vectors, 2012). We also examined the effect of temperature on replication of EHDV-7 in C. sonorensis and demonstrated that the time to detection of potentially competent midges decreased with increasing temperature (Ruder et al. in preparation). Although multiple attempts were made, we failed to evaluate wild-caught Culicoidesinsignisas a potential vector for EHDV-7; however, our finding that C. sonorensis is a competent vector is far more significant because this species is widespread in the U.S. As for Israeli Culicoides spp. the main species caught near farms affected during the outbreaks were C. imicolaand C. oxystoma. The vector competence studies performed in Israel were in a smaller scale than in the US due to lack of a laboratory colony of these species and due to lack of facilities to infect animals with vector borne diseases. However, we found both species to be susceptible for infection by EHDV. For C. oxystoma, 1/3 of the Culicoidesinfected were positive 11 days post feeding. 3. To identify the host and environmental factors influencing the level of exposure to EHDV, its spread and its associated morbidity: Analysis of the cattle morbidity in Israel showed that the disease resulted in an average loss of over 200 kg milk per cow in herds affected during September 2006 and 1.42% excess mortality in heavily infected herds (Kedmi et al. Journal of Dairy Science, 2010). Outbreak investigation showed that winds played a significant role in virus spread during the 2006 outbreak (Kedmi et al. Preventive Veterinary Medicine, 2010). Further studies showed that both sheep (Kedmi et al. Veterinary Microbiology, 2011) and wild ruminants did not play a significant role in virus spread in Israel (Kedmi et al. article in preparation). Clinical studies in WTD showed that this species is highly susceptibile to EHDV-7 infection and disease (Ruder et al. Journal of Wildlife Diseases, 2012). Experimental infection of Holstein cattle (cows and calves) yielded subclinical viremia (Ruder et al. in preparation). The findings of this study, which resulted in 6 articles, published in peer reviewed journals and 4 more articles which are in preparation, contributed to the dairy industry in Israel by defining the main factors associated with disease spread and assessment of disease impact. In the US, we demonstrated that sufficient conditions exist for potential virus establishment if EHDV-7 were introduced. The significant knowledge gained through this study will enable better decision making regarding prevention and control measures for EHDV and similar viruses, such as BTV.