Teses / dissertações sobre o tema "Perennial pastures"

Cool season perennial grasses are the foundation of equine nutrition in the transition zone. The objective of this study was to evaluate forage quality using ADF, NDF, IVTDMD, CP, WSC, and ESC and changes in vegetative swards seasonally, diurnally, across species (Kentucky bluegrass, tall fescue, orchardgrass, and perennial ryegrass) and cultivar. This study was conducted in 2015 and 2017 and plots were maintained vegetatively with two to four week mowing. Morning and afternoon sample collection occurred monthly during the growing season. Samples were flash frozen; freeze dried, ground, and scanned using Near Infrared Reflectance Spectroscopy (NIRS) to predict forage quality. There was a significant year effect; therefore year was analyzed separately. Generally, ADF and NDF were highest for Kentucky bluegrass (30 and 52%), lowest for perennial ryegrass (25 and 46%), and tall fescue and orchardgrass were inconsistent. Crude protein was variable across species and season, ranging 10 to 25%. ADF and NDF concentrations were higher in the morning; IVTDMD, WSC, and ESC were higher in the afternoon; and CP was similar diurnally. In conclusion, forage quality in vegetative cool season grass pastures was sufficient to meet the nutritional needs of most equines, but varied seasonally, diurnally, across species, and cultivar.

Grazing behaviour of domestic horses on perennial ryegrass pastures and the effect of sward height On paddocks used for horse grazing, swards are often depleted by overgrazing and patches of bare ground appear due to resting and walking behaviour of the animals. In order to improve pasture production and animal management the knowledge of grazing and social behaviour is essential. The present study therefore investigated grazing behaviour of horses and their response to different sward heights and the influence of being a herd animal on individual behaviour. The experiments performed showed that horses perceive differences in sward height and that they increase bite dimensions, such as bite depth, volume, area and mass, as sward height increases. As bite depth slightly increased when the horses' whiskers around the rilUzzle were trimmed or shaved, it was shown that the whiskers play an important role in perceiving the distance of the muzzle to the vegetation. For reasons of animal welfare, it is suggested that the practice of whisker treatment is stopped. When presented with a choice between different sward heights, horses selected tall swards (> 8.2 cm) which supply the highest bite mass. However, when young grass leaves overtop the older ones on shorter areas, horses also graze these short patches, indicating that they switch between energy maximisation per bite and quality of food. When sward height declined, a slight increase in bite rate was observed. It is therefore suggested that when bite mass is constrained by sward height horses may increase bite rate in an attempt to maintain a constant gut fill. An increase in the total time grazing per day as a response to a decrease in sward height was not detected. Due to the observed impact of gregarious behaviour on grazing and resting behaviour of individual horses, it is suggested that horses should be kept in groups of at least two animals.

Over the past thirty years, price relativities and technological development have motivated an increase in the area of land allocated to cropping, as opposed to pasture production, throughout the central wheat belt of Western Australia. Nevertheless, reducing the proportion of pasture in these rotations has challenged the future productivity of farming systems in this area. First, the frequent application of selective herbicides for weed control in extended cropping rotations has promoted the development of herbicide resistance in a number of major agricultural weeds. Second, the primary use of annual plants has promoted the development of soil salinisation by allowing a significant proportion of rainfall to recharge saline water tables. The inclusion of perennial pasture phases between extended periods of cropping may mitigate or delay these constraints to production through (a) allowing the use of costeffective forms of non-selective weed control, and (b) through creating a buffer of dry soil that absorbs leakage occurring beneath subsequent crops. This study consequently explores the value of including perennial pasture phases in dryland agricultural systems in the eastern-central wheat belt of Western Australia, accounting for benefits related to herbicide resistance and water table management. A novel computational algorithm for the solution of multiple-phase optimal control problems is developed and used to conduct a conceptual analysis of the value of lucerne (Medicago sativa L.) pasture for managing annual ryegrass (Lolium rigidum Gaudin), the primary weed in wheat belt cropping systems. The competitiveness and fecundity of annual ryegrass provide strong economic incentives to maintain a low weed population, irrespective of herbicide-resistance status. Consequently, the ineffectiveness of selective herbicides primarily reduces the profitability of cropping by motivating the adoption of more costly non-selective forms of weed control. The inclusion of lucerne in land-use rotations is only optimal in the presence of severe herbicide resistance given (a) the low efficiency of alternative weed-management practices available during the pasture phase, relative to selective-herbicide application; (b) the significant cost of establishing this perennial pasture; and (c) the high relative profitability of cereal production in the absence of resistance. The value of lucerne, relative to annual pastures, for weed management is explored in greater detail through the use of compressed annealing to optimise a sophisticated simulation model. The profitability of candidate rotations is also manipulated to account for the long-term production losses accruing to the recharge of saline groundwaters that occurs beneath them. Sequences incorporating lucerne are only more profitable than those that include annual pasture at the standard set of parameter values if (a) annual ryegrass is resistant to all selective herbicides, (b) the water table is so shallow (approximately less than 3.5 m deep) that frequent rotation with perennials is required to avert soil salinisation, or (c) sheep production is highly profitable. The value of perennial pasture is sufficient under these circumstances to overcome its high establishment cost. Consistent with intuition, these benefits are reinforced by lower discount rates and higher rates of leakage occurring beneath annual-based systems. Formulation of an effective communication strategy to report these results to producers is justified given the complexity involved in determining the true magnitude of these intertemporal benefits through alternative means, such as field trials.

Thesis (MSc. (Pasture Science)) -- University of Limpopo, 2016
South Africa is regarded as a semi-arid area; only 28% of the country receives more than 600 mm rainfall recorded annually. Sustainable utilization of cool season fodder grasses in summer rainfall areas to produce winter animal feed remains a major problem. Farmers are affected by a shortage of adequate, good quality herbage for livestock during winter and dry periods on commercial farms. The study aimed to evaluate and compare the production potential of six Festulolium hybrids, three Dactylis species, and five Lolium hybrids and three grass-legume mixtures in the summer rainfall area. A total of 17 cultivars were evaluated for their DM production under the treatment of different fertilizer levels on three planting dates (16 March 2011, 21 April 2011 and 4 April 2012). The study was carried out at Hygrotech Seed Company (Experimental site), Dewagensdrift in Moloto Village outside Pretoria in the Gauteng Province. Data was collected on a monthly basis from sub-plots of 1 m x 1 m (m2) in 51 plots of 1.5 m x 6 m (9 m2), with nine rows that were 10 cm apart arranged under RCBD with three replicates per cultivar. Harvested fresh samples were taken, weighed, dried at 60ºC until they reached a constant dry weight and weighed to determine DM content. A Fischer’s protected LSD at the 5 % level was performed to compare the treatment means. Over a period of three years of study, it shows that the low fertilizer level resulted in the lowest DM production, with no significant difference occurring between medium and high fertilizer levels. To achieve optimum DM production with minimum cost medium fertilizer is recommended. The cultivars responded differently to the three different planting dates. The time of planting had an effect on DM production in winter. It is recommended that Festulolium hybrids and Lolium hybrids be planted earlier (March) for better DM production in winter.
National Research Foundation (NRF)

Alternative grazing systems and pasture types for wool production in the south west of Western Australia were analysed using bio-economic modelling techniques in order to determine their relative productivity and profitability. After reviewing the experimental and modelling literature on perennial pastures and grazing systems, seven case studies of farmers were conducted in order to investigate the practical application of innovative grazing systems and use of perennial pastures. Together these case studies provided information for identifying relevant variables and for calibrating the modelling work which followed. The core of the work lies in a bio-economic model for investigating the comparative value of the three grazing systems and two pasture families mentioned above. A baseline scenario using currently available and reliable scientific data provides baseline results, after which a number of sensitivity analyses provide further insights using variations of four key parameters: persistence, heterogeneity, water soluble carbohydrates, and increased losses. Results show that perennial pastures are in the studied region more profitable than annual pastures. Under current baseline conditions, continuous grazing with perennial pastures is the most profitable enterprise, but this superiority is not robust under parameter variations defined by other scenarios. The more robust solution in terms of enterprise profitability is cell grazing with perennial pastures. The results indicate that intensive grazing systems such as cell grazing have the potential to substantially increase the profitability of grazing operations on perennial pasture. This result is an encouraging one in light of its implications for water uptake and salinity control. It means that economics and land care can go hand in hand, rather than be competitive. It is to be noted that it is the choice of the grazing system in combination with the pasture species, rather then the pasture species itself, that allows for such complementarity between economics and sustainable land use. This research shows that if farmers adopt practices such as cell grazing they may be able to increase the area that they can profitably plant to perennial pasture thus reducing the impacts of dryland salinity. This finding is consistent with the findings of the case studies where the farmers perceived that, provided grazing was planned, increasing the intensity of their grazing management and the perenniallity of their pastures would result in an increase in the profitability of their grazing operation. As a result this research helps to bridge a gap which has existed in this area of research, between the results of scientific research and those reported in practice.

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior
In Brazil, most soils used in the livestock presents some fertility limitations. One of the alternatives to improve the soils quality is the introduction of perennial species, including grassy and associated legumes and correction of the soil that it will hold the cultures forage crop. There s a lack of Information and researches on soil quality improvement using perennial forages. Therefore, the objective of this work was to evaluate different systems of forage cultures constituted by Pennisetum americanum, Arachis pintoi and Stylosanthes guianensis associated with Cynodon dactylon and both isolated species, with and without mineral fertilizer, and evaluations of the attributes of a Ultisols. In both treatments, Cynodon dactylon received 100 kg of nitrogen during a year. The crops of forage samples were accomplished at every 28 days, making botanical separation and drying the same. After the weighting to calculate the dry matter per area (ha) the samples were crushed for analyses of fiber of neutral detergent (FDN), fiber of acid detergent (FDA), rude protein (PB), calcium (Ca), magnesium (Mg), phosphorus (P) and potassium (K). The samples collecting were taken during January, March, April and October of 2007. The best results of nutritional values were found in the legumes and in the consortium, being with inferior quality the grassy ones. The dry matter present a great variation among the cuts and the forage systems. After the winter the Stylosanthes portions were lost, due to the frosts that happened during this period. This confirms that it not has an adaptation on our conditions of winter. After a year of implantation of the experiment was collected soil to analysis: density of the soil (DS), microporosity (Mip) and macroporosity (Map) porosity and total porosity of the soil (PT) and size of aggregates. In the chemical part, were analyzed total nitrogen (NT), total organic carbon (COT) and phosphorus (P) and potassium (K). DS presented variation between the treatments and the appraised layers, since 1,40 to 1,62 Mg m-3. In Mip and PT didn't present significant differences between the treatments and layers, but Map presented significant differences. In the aggregate size there was significant differences, and the geometric medium diameter (DMG) it varied from 1,41 to 3,21 mm. In the soil chemical attributes both maintained similarities, in other words, larger concentration in the first layer (0 to 5 cm). But some treatments standing out in larger concentration in the first layer, however maintain similarities in the deep layers, unless the potassium (K), that has a distribution more constantly in the evaluated layers.
No Brasil, a maioria dos solos utilizados para pecuária apresenta algumas limitações de fertilidade. Uma das alternativas para melhorar as condições desses solos é a introdução de espécies perenes, incluindo gramíneas e leguminosas consorciadas e correção do solo que comportará as culturas forrageiras. Informações e pesquisas sobre melhorias de solos com uso de espécies forrageiras perenes são escassas. Nesse sentido, o objetivo deste trabalho foi avaliar diferentes sistemas de culturas forrageiras constituídas de milheto, amendoim forrageiro e estilosantes consorciados com tifton 85 e ambas as espécies isoladas, com e sem adubação mineral, e avaliações dos atributos de um Argissolo vermelho. Em ambas as parcelas adubadas e não adubadas, o tifton 85 recebeu 100 kg de nitrogênio ano-1. As colheitas de amostras de forragem eram realizadas a cada 28 dias, realizando-se separação botânica e secagem da mesma. Feita a pesagem para calcular matéria seca (MS) por área (ha) realizava-se a moagem das amostras para análises de fibra de detergente neutro (FDN), fibra de detergente ácido (FDA), proteína bruta (PB), cálcio (Ca), magnésio (Mg), fósforo (P) e potássio (K). Realizaram-se coletas nos meses de janeiro, março, abril e outubro de 2007. Os melhores resultados de valores de PB, FDA e FDN, foram encontrados nas leguminosas e no consórcio, ficando com qualidade inferior o tifton 85 + 100 kg ha-1 de N. Quanto à produção de MS, variou muito entre os cortes e os sistemas forrageiros. Após o inverno as parcelas de estilosantes foram perdidas, devido às geadas que ocorreram no período de inverno. Isso confirma que o mesmo não tolera frio e não se adapta a nossas condições de inverno. Após um ano de implantação do experimento realizou-se coletas para análise dos atributos do solo, sendo: densidade do solo (DS), micro (Mip) e macro (Map) porosidade e porosidade total do solo (PT) e tamanho de agregados. Na parte química, analisou-se nitrogênio total (NT), carbono orgânico total (COT) e teores de fósforo (P) e potássio (K). A DS apresentou variação entre os tratamentos e as camadas avaliadas, desde 1,40 a 1,62 Mg m-3. Na Mip e PT não apresentou diferenças significativas entre os tratamentos e camadas, mas o Map apresentou diferenças significativas. Nos tamanhos dos agregados houve diferenças significativas, sendo que o diâmetro médio geométrico (DMG) variou de 1,41 a 3,21 mm. Nos atributos químicos do solo, como NT, COT, P e K ambos mantiveram semelhanças, ou seja, maior concentração na primeira camada (0 a 5 cm). Mas alguns tratamentos se destacaram em maior concentração na primeira camada, mantendo semelhanças nas camadas mais profundas, exceto o K que teve uma distribuição mais uniforme nas camadas avaliadas.

Soil P fractionation was used to examine changes in soil inorganic and organic P under grazed irrigated pasture in a long-term field trial at Winchmore in Mid-Canterbury. The soil P fractionation scheme used involved sequential extractions of soil with O.5M NaHCO₃ @ pH 8.5 (NaHCO₃ P), 0.1M NaOH (NaOH I P), 1M HCl (HCl P) and 0.1M NaOH (NaOH II P). The Winchmore trial comprised 5 treatments: control (no P since 1952), 376R (376 kg superphosphate ha⁻¹ yr⁻¹ 1952-1957, none since), 564R (564 kg superphosphate ha⁻¹ yr⁻¹ 1952-1957, none since) 188PA (188 kg superphosphate ha⁻¹ yr⁻¹ since 1952) and 376PA (376 kg superphosphate ha⁻¹ yr⁻¹ since 1952: Topsoil (0-7.5cm) samples taken from the different treatments in 1958, 1961, 1965, 1968, 1971, 1974 and 1977 were used in this study. Changes in soil P with time showed that significant increases in soil inorganic P occurred in the annually fertilised treatments (l88PA, 376PA). As expected, the overall increase in total soil inorganic P between 1958 and 1977 was greater in the 376PA treatment (159 µg P g⁻¹) than that in the 188PA treatment (37 µg P g⁻¹). However, the chemical forms of inorganic P which accumulated in the annually fertilised treatments changed with time. Between 1958 and 1971 most of the increases in soil inorganic P in these treatments occurred in the NaHCO₃ and NaOH I P fractions. On the other hand, increases in soil inorganic P in the annually fertilised treatments between 1971 and 1977 were found mainly in the HCl and NaOH II P fractions. These changes in soil P forms were attributed to the combined effects of lime addition in 1972 and increased amounts of sparingly soluble apatite P and iron-aluminium P in the single superphosphate applied during the 1970's. In the residual fertiliser treatments (376R, 564R) significant decreases in all of the soil inorganic P fractions (i.e. NaHCO₃ P, NaOH I P, HCl P, NaOH II p) occurred between 1958 and 1977 following the cessation of P fertiliser inputs in 1957. This was attributed to continued plant uptake of P accumulated in the soil from earlier P fertiliser additions. However, levels of inorganic P in the different soil P fractions in the residual fertiliser treatments did not decline to those in the control which indicated that some of the inorganic P accumulated in the soil from P fertiliser applied between 1952 and 1957 was present in very stable forms. In all treatments, significant increases in soil organic P occurred between 1958 and 1971. The overall increases in total soil organic P were greater in the annually fertilised treatments (70-86 µg P g⁻¹) than those in the residual fertiliser (55-64 µg P g⁻¹) and control (34 µg P g⁻¹) treatments which reflected the respective levels of pasture production in the different treatments. These increases in soil organic P were attributed to the biological conversion of native and fertiliser inorganic P to organic P in the soil via plant, animal and microbial residues. The results also showed that annual rates of soil organic P accumulation between 1958 and 1971 decreased with time which indicated that steady-state conditions with regard to net 'organic P accumulation were being reached. In the residual fertiliser treatments, soil organic P continued to increase between 1958 and 1971 while levels of soil inorganic P and pasture production declined. This indicated that organic P which accumulated in soil from P fertiliser additions was more stable and less available to plants than inorganic forms of soil P. Between 1971 and 1974 small (10-38 µg P g⁻¹) but significant decreases in total soil organic P occurred in all treatments. This was attributed to increased mineralisation of soil organic P as a result of lime (4 t ha⁻¹) applied to the trial in 1972 and also to the observed cessation of further net soil organic P accumulation after 1971. Liming also appeared to affect the chemical nature of soil organic P as shown by the large decreases in NaOH I organic P(78-88 µg P g⁻¹) and concomitant smaller increases in NaOH II organic P (53-65 µg P g⁻¹) which occurred in all treatments between 1971 and 1974. The chemical nature of soil organic P in the Winchmore long-term trial was also investigated using 31p nuclear magnetic resonance (NMR) spectroscopy and gel filtration chromatography. This involved quantitative extraction of organic P from the soil by sequential extraction with 0.1M NaOH, 0.2M aqueous acetylacetone (pH 8.3) and 0.5M NaOH following which the extracts were concentrated by ultrafiltration. Soils (0-7.5cm) taken from the control and 376PA annually fertilised treatments in 1958, 1971 and 1983 were used in this study. 31p NMR analysis showed that most (88-94%) of the organic P in the Winchmore soils was present as orthophosphate monoester P while the remainder was found as orthophosphate diester and pyrophosphate P. Orthophosphate monoester P also made up almost all of the soil organic P which accumulated in the 376PA treatment between 1958 and 1971. This indicated that soil organic P in the 376PA and control treatments was very stable. The gel filtration studies using Sephadex G-100 showed that most (61-83%) of the soil organic P in the control and 376PA treatments was present in the low molecular weight forms (<100,000 MW), although the proportion of soil organic P in high molecular weight forms (>100,000 MW) increased from 17-19% in 1958 to 38-39% in 1983. The latter was attributed to the microbial humification of organic P and indicated a shift toward more complex and possibly more stable forms of organic P in the soil with time. Assuming that the difference in soil organic P between the control and 376PA soils sampled in 1971 and 1983 represented the organic P derived from P fertiliser additions, results showed that this soil organic P was evenly distributed between the high and low molecular weight fractions. An exhaustive pot trial was used to examine the relative availability to plants of different forms of soil inorganic and organic P in long-term fertilised pasture soils. This involved growing 3 successive crops of perennial ryegrass (Lolium perenne) in 3 Lismore silt loam (Udic Ustochrept) soils which had received different amounts of P fertiliser for many years. Two of the soils were taken from the annually fertilised treatments in the Winchmore long term trial (188PA, 376PA) and the third (Fairton) was taken from a pasture which had been irrigated with meatworks effluent for over 80 years (65 kg P ha⁻¹ yr⁻¹). Each soil was subjected to 3 treatments, namely control (no nutrients added), N100 and N200. The latter treatments involved adding complete nutrient solutions with different quantities of N at rates of 100kg N ha⁻¹ (N100) and 200kg N ha⁻¹ (N200) on an area basis. The soil P fractionation scheme used was the same as that used in the Winchmore long-term trial study (i.e. NaHCO₃ P, NaOH I P, HCl P, NaOH II p). Results obtained showed that the availability to plants of different extracted inorganic P fractions, as measured by decreases in P fractions before and after 3 successive crops, followed the order: NaHCO₃ P > NaOH I P > HCl P = NaOH II P. Overall decreases in the NaHCO₃ and NaOH I inorganic P fractions were 34% and 16% respectively, while corresponding decreases in the HCl and NaOH II inorganic P fractions were small «10%) and not significant. However, a significant decrease in HCl P (16%) was observed in one soil (Fairton-N200 treatment) which was attributed to the significant decrease in soil pH (from 6.2 to 5.1) which occurred after successive cropping. Successive cropping had little or no effect on the levels of P in the different soil organic P fractions. This indicated that net soil organic P mineralisation did not contribute significantly to plant P uptake over the short-term. A short-term field experiment was also conducted to examine the effects of different soil management practices on the availability of different forms of P to plants in the long-term fertilised pasture soils. The trial was sited on selected plots of the existing annually fertilised treatments in the Winchmore long-term trial (188PA, 376PA) and comprised 5 treatments: control, 2 rates of lime (2 and 4 t ha⁻¹ ) , urea fertiliser (400kg N ha⁻¹ ) and mechanical cultivation. The above ground herbage in the uncultivated treatments was harvested on 11 occasions over a 2 year period and at each harvest topsoil (0-7.5 cm) samples were taken from all of the treatments for P analysis. The soil P fractionation scheme used in this particular trial involved sequential extractions with 0.5M NaHCO₃ @ pH 8.5 (NaHCO₃ P), 0.1M NaOH (NaOH P), ultrasonification with 0.1M NaOH (sonicate-NaOH p) and 1M HCl (HCl P). In addition, amounts of microbial P in the soils were determined. The results showed that liming resulted in small (10-21 µg P g⁻¹) though significant decreases in the NaOH soil organic P fraction in the 188PA and 376PA plots. Levels of soil microbial P were also found to be greater in the limed treatments compared with those in the controls. These results indicated that liming increased the microbial mineralisation of soil organic P in the Winchmore soils. However, pasture dry matter yields and P uptake were not significantly affected. Although urea significantly increased dry matter yields and P uptake, it did not appear to significantly affect amounts of P in the different soil P fractions. Mechanical cultivation and the subsequent fallow period (18 months) resulted in significant increases in amounts of P in the NaHCO₃ and NaOH inorganic P fractions. This was attributed to P released from the microbial decomposition of plant residues, although the absence of plants significantly reduced levels of microbial P in the cultivated soils. Practical implications of the results obtained in the present study were presented and discussed.

Despite the importance of a high white clover (Trifolium repens) content in temperate pastoral systems in terms of livestock performance and nitrogen fixation, the proportion of white clover in grass-clover pastures is often low (<20%). This thesis examined in two experiments whether the white clover content of pastures could be improved by sowing white clover with alternative grass species to diploid perennial ryegrass (Lolium perenne L.). In a pasture experiment, DM production, pasture composition and morphology of grass-clover mixtures was measured over the establishment year (January 2007 to January 2008) where white clover was sown in fine mixtures with diploid perennial ryegrass, tetraploid perennial ryegrass, timothy (Phleum pratense L.) and cocksfoot (Dactylis glomerata L.). Pastures were irrigated and rotationally grazed with on-off grazing with Coopworth ewe hoggets. Total annual DM production of pasture was more than 20% higher in tetraploid (12521 kg DM ha⁻¹) and diploid (11733 kg DM ha⁻¹) perennial ryegrass than timothy (9751 kg DM ha⁻¹) and cocksfoot (9654 kg DM ha⁻¹). However, timothy (5936 kg DM ha⁻¹) and cocksfoot (5311 kg DM ha⁻¹) had more than four times higher white clover annual DM production than tetraploid (1310 kg DM ha⁻¹) and diploid (818 kg DM ha⁻¹) ryegrass. Pasture growth rate at the first three harvests in autumn was significantly greater in tetraploid and diploid ryegrass than timothy and cocksfoot. Timothy and cocksfoot had a higher proportion of white clover than tetraploid and diploid perennial ryegrass throughout the entire year. This was due to more and larger white clover plants in timothy and cocksfoot plots. In a grazing preference experiment, the partial preference of sheep for white clover offered in combination with the same grass species as in the pasture experiment was measured in five grazing tests in May, September, October, November and December 2007. Pastures were sown in January 2007. Paired plots (grass and clover both 4.2 m x 10 m) were grazed by three Coopworth ewe hoggets between 9am and 5pm, and preference was recorded by decline in pasture mass and visual scan sampling for grazing time. Grazing preference for clover was generally low throughout these tests (e.g. average apparent DM intake from clover = 47%; average grazing time from clover = 44%). Several explanations are proposed for this low preference including a high N content and intake rate of the grass relative to the clover. No significant differences were found among the grass treatments in total grass grazing time, total clover grazing time, ruminating time, the proportion of grazing time on clover, selective coefficient for clover and DM intake percentage from clover at any date. There was no significant change in overall sward surface height (SSH) decline among grass treatments throughout all the tests except December 2007 when the overall SSH decline for cocksfoot was significantly lower than the other species. The study indicated that the rapid growth rate of perennial ryegrass in the early phase of pasture establishment, rather than differences in partial preference, was the key factor limiting white clover content in the mixed swards relative to cocksfoot and timothy pastures. It is concluded that high clover-containing pastures capable of delivering high per head performance can be established through the use of slow establishing pasture species such as timothy and cocksfoot.

[Truncated abstract] Salinity and waterlogging interact to reduce the growth of most crop and pasture species. Species that are productive on saline-waterlogging land are needed for Australian farming systems. One option is Lotus tenuis, a perennial legume widely grown for pasture in the flood-prone and salt-affected Pampa region of Argentina. To identify mechanisms responsible for the adverse interaction between salinity and waterlogging, Lotus tenuis with a reputation for tolerance was compared with L. corniculatus, the most widely cultivated Lotus species. The physiology of salt and waterlogging tolerance in L. tenuis (4 cultivars) was evaluated, and compared with L. corniculatus (3 cultivars). Overall, L. tenuis cultivars accumulated less Na+ and Cl-, and more K+ in shoots than L. corniculatus cultivars, when exposed to 200 mM NaCl for 28 d in aerated or in anoxic (stagnant agar) solutions. In a NaCl dose response experiment (0 to 400 mM NaCl in aerated solution), Lotus tenuis (cv. Chaja) accumulated half as much Cl- in its shoots than L. corniculatus (cv. San Gabriel) at all external NaCl concentrations, and about 30% less shoot Na+ in treatments above 250 mM NaCl. Ion distributions in shoots were determined for plants at 200 mM NaCl; L. tenuis (cv. Chaja) accumulated about half as much Cl- in old leaves, young leaves and stems, compared with concentrations in L. corniculatus (cv. San Gabriel). There were not, however, significant differences between the two species for Na+ concentrations in the various shoot tissues under aerated NaCl treatment. '...' Therefore, during early stages of exposure to salinity, L. tenuis accumulated a higher proportion of total Na+ in the roots under combined stagnant-plus-NaCl treatment (55% versus 39% for L. corniculatus). Na+ transporters, particularly those relying on H+ gradients across membranes, which in turn require adequate ATP levels, could be impaired under O2 deficits that inhibit respiration. To study the effect of O2 deficiency on a Na+ transporter, an NHX1-like gene was cloned from L. tenuis and identity established via sequencing and yeast complementation studies. Real-time qPCR showed expression of NHX1 in L. tenuis roots increased under stagnant-plus-NaCl treatment, whereas it was reduced in L. corniculatus. Thus, maintaining O2 transport to roots, together with up-regulation of an NHX1-like gene for Na+ accumulation in vacuoles, contributes to tolerance of L. tenuis to combined salinity and waterlogging stresses. This study highlights the importance of minimising Cl- transport to shoots as a mechanism of salt tolerance and has identified a CCC-like gene in L. tenuis as a candidate for mediating root-to-shoot Cl- transport. Under combined stagnant-plus-NaCl treatment, control of Na+ transport is another mechanism contributing to tolerance in these Lotus species. Enhanced root aeration in L. tenuis maintains root Na+ transport processes, such as accumulation in vacuoles via NHX1-like genes, to diminish xylem loading to the shoot. Overall, this thesis has contributed new knowledge on the potential of Lotus tenuis as a saltland pasture and has significantly enhanced current understanding on the mechanisms of salinity and waterlogging tolerance in plants.

Sod-seeding techniques offer graziers a convenient way to introduce superior grass cultivars into underproductive permanent pastures. Production loss and erosion are minimized. In conjunction with improved grazing management and fertilization, renovation can significantly improve yield and quality of pastures. Existent vegetation must be suppressed prior to introducing new cultivars. This study was conducted on two non-irrigated pastures near Corvallis, Oregon, one dominated by annual grass species and the other by perennial grasses and clover. A split-plot design with four replications on each site was used to compare three seeding methods and either (a) two herbicides following close mowing or (b) close mowing alone. The seeding methods were drilling with an Aerway Seedmatic chisel-type drill, drilling with a Tye double disc drill, or broadcasting seed followed by harrowing. Glyphosate and paraquat were the herbicides used for vegetation suppression. Effect of fertilization was compared to no fertilization. Sod-seeded perennial ryegrass had minimal establishment at the site dominated by annual grass species. An inadequate amount of time was allowed for germination of annual grass seeds before herbicides were applied. Annual grass seedlings suppressed the newly sod-seeded perennial ryegrass. Sod-seeded perennial ryegrass was successfully established at the site dominated by perennial species within one year after planting. Broadcasting followed by harrowing of seed resulted in a higher percentage of perennial ryegrass than either the Seedmatic chisel drill or Tye double disc drill. Sod-seeded perennial ryegrass did not contribute significantly to yield until one year after planting. Glyphosate gave better control of the species present before planting leading to a higher percentage of perennial ryegrass and improved yield compared to paraquat or close mowing alone when seed was broadcast and harrowed. Fertilization of unseeded plots increased yield but was not cost-effective.
Graduation date: 1994

"May 2005"
Bibliography: leaves 202-220.
viii, 220 leaves : ill. (some col.), maps (chiefly col.), plates, photographs (chiefly col.) ; 30 cm.
Title page, contents and abstract only. The complete thesis in print form is available from the University Library.
Thesis (Ph.D.)--University of Adelaide, School of Agriculture and Wine, Dept. of Agronomy and Farming Systems, 2005

Sequestering atmospheric carbon in soil is an attractive option for mitigation of rising atmospheric carbon dioxide concentrations through agriculture. Perennial crops are more likely to gain carbon while annual crops are more likely to lose carbon. A pair of eddy covariance towers were set up near Winnipeg Manitoba, Canada to measure carbon flux over adjacent fertilized long-term perennial grass hay fields with high soil organic carbon. In 2009 the forage stand of one field (Treatment) was sprayed with herbicide, cut and bailed; following which cattle manure was applied and the land was tilled. The forage stand in the other field (Control) continued to be cut and bailed. Differences between net ecosystem productivity of the fields were mainly due to gross primary productivity; ecosystem respiration was similar for both fields. When biomass removals and manure applications are included in the carbon balance, the Treatment conversion lost 149 g C m^(-2) and whereas the Control sequestered 96 g C m^(-2), for a net loss of 245 g C m^(-2) over the June to December period (210 days). This suggests that perennial grass converted for annual cropping can lose more carbon than perennial grasses can sequester in a season.

Condensed tannins (CT) play an integral role in terrestrial nutrient cycling. Despite being the fourth most abundant terrestrial biochemical product, the regulatory processes of tannin production in plants and their subsequent ecological influences are not completely understood. The defining characteristic of CT’s is their affinity for proteins, though they willingly interact with minerals, carbohydrates, and other polyphenolic compounds. Previous tannin investigations in agriculture have centred on bioactivity related to ruminant digestive physiology and pathology. These studies have revealed that CT’s have the potential to increase liveweight gain, wool production, and sheep ovulation rates, prevent pasture bloat, reduce enteric and stockpiled manure greenhouse gas (GHG) emissions, and control gastrointestinal parasites. The present study has explored how tannin-containing resident plant material and applied manure derived from tannin-containing beef cattle diets influences tame forage biomass yield, proximate analysis, and tannin production, as well as residual fall soil nutrient status, in southern Manitoba. To test these hypotheses, we conducted an experiment over two growing seasons (2007-2008) where in the fall of 2007, both tannin-derived (sainfoin, Onobrychis viciifolia) and non-tannin (alfalfa, Medicago sativa) composted beef manures were applied at a rate of 44.8 t/ha in a randomized split plot fashion, including a manure-free control, on a four repetition randomized complete block design which included both tannin-containing and non-tannin containing forage monocultures and mixtures. Plant samples were harvested in two cuts, and soil measurements were taken in the fall of both years. It was found that across all sainfoin treatments, manure origin did not have a significant effect on plant CT concentration, which was extremely variable within treatments and cuts. Over four harvest dates, sainfoin CT concentrations ranged from 14.1 g/kg to 91.9 g/kg in monoculture plots, and 16.6 g/kg to 123 g/kg when grown in a mixture with meadow brome. In most cases, the presence of either manure type did not significantly affect soil nutrient status or forage yield, though results were similarly variable. Using a stepwise regression which included all soil and plant measurements across all cuts and treatments, it was found that NDF (R2 = 0.548) and plant phosphorus (R2 = 0.126) were the only significant model contributors to tannin concentration in sainfoin at P < 0.15. These findings suggest that nutrient effects of beef cattle manure are not realized in either plant or soil in the year following application, and consequently, that tannin agronomy requires longer-term analysis.

Two field grazing experiments were conducted in New Zealand (NZ) in spring (Experiment 1; November 2008) and autumn (Experiment 2; April 2009) to evaluate the effects of feeding a high sugar perennial ryegrass (HSG; cv. AberDart; derived in the United Kingdom; UK) versus a NZ- derived control grass (cv. Impact) on milk production and estimated nitrogen (N) partitioning within the cow. Areas of both ryegrasses were replicated and sown with or without white clover (cl) (HSG+cl, control+cl, HSG and control). A cross-over design with four 10-day periods was used in each experiment, using 15 Friesian cows per treatment per period in Experiment 1 and 5 cows per treatment per period in Experiment 2. Treatment effects upon pasture botanical and chemical composition, cows’ milk yield and composition, and estimated N partitioning were studied. Nitrogen partitioning was calculated using indirect methods. Herbage concentrations of water soluble carbohydrates (WSC) were lower in autumn than in spring whilst crude protein (CP) concentrations were higher in autumn. Organic matter digestibility (OMD) and metabolisable energy (ME) concentration was similar in both seasons. There were no differences in the concentration of CP, WSC and dry matter (DM) among treatments in Experiment 1. The HSG+cl treatment had the lowest concentrations of neutral detergent fibre (NDF, 417 g/kg DM) and the highest content of ME (12.6 MJ/kg DM) and tended to have the lowest sward dead matter content compared with the other three treatments. In Experiment 2 both HSG treatments showed higher concentrations of WSC (15 g/kg DM) compared with the control, both with and without clover; the concentrations of NDF and acid detergent fibre (ADF) were the lowest for both HSG treatments. In Experiment 1, cows grazing treatments with white clover produced more milk (1.6 kg/day) and more milk solids (MS; 0.16 kg/day) than cows grazing pure ryegrass swards ( P< 0.01), with highest milk yields being from cows grazing the HSG+cl treatment (ryegrass cultivar x white clover interaction P<0.05). No differences in milk production were found in Experiment 2. Estimated urinary N excretion (g/day) was similar for all treatments in both seasons, although N intake differed among treatments. The proportion of N intake excreted in urine or secreted in milk was similar for all treatments in both experiments. Nitrogen output (g/day) in milk was the highest for the HSG+cl treatment in Experiment 1 but no differences were found in Experiment 2. Data were combined from both experiments to study the effects of the herbage CP:WSC ratio upon estimated N partitioning between milk and urine. Mean ratios were 0.72 for spring herbage and 2.27 for autumn herbage. As the amount of WSC increased in the diet relative to the amount of CP (thus a lower CP:WSC ratio) there was a significant increase in the amount of milk N secreted per unit of N intake in spring but not in autumn. The breakpoint in the relationship between the herbage CP:WSC ratio and the nitrogen utilisation efficiency for milk production (NUEm) was 1.32, and the NUEm for that breakpoint was 14 g milk N per 100 g N intake. Ratios below this point were associated with improved efficiency of converting pasture N to milk N; ratios above this point were not correlated with changes in N conversion efficiency. It is concluded that the CP:WSC ratio in perennial ryegrass may be important in the partition of absorbed N into milk or urine. A NZ-selected HSG with a lower CP:WSC ratio is likely to have major benefits for pastoral farming in NZ. In order to be effective, a NZ-derived HSG should substantially increase WSC concentration in autumn pasture (from approximately 100 to 200 g/kg DM) whilst reducing CP content simultaneously (from 240 to 190 g/kg DM). The lower structural fibre and higher milk production for the HSG+cl treatment in both experiments suggest that under NZ conditions, best productive responses to HSG may be obtained in management systems that include white clover.

This thesis defines digestion kinetics for perennial ryegrass (Lolium perenne L.), which is the main component of diets fed to dairy cows in New Zealand. Chemical composition and digestion kinetics were measured in fresh minced ryegrass as it matured and leaf, stem and inflorescence of several grass species. In sacco and in vitro incubations were used to define rates of degradation and nutrient release. Two short-term grazing trials were used to evaluate contrasting silages as supplements for cows fed restricted amounts of summer pasture. The minced preparation of ryegrass resulted in a similar distribution of dry matter (DM) between particle size fraction and rumen digesta from cows fed pasture. Mincing released 0.46 – 0.80 of crude protein into the soluble fraction, with highest proportions for mature grasses which had low CP concentrations (about 8 g CP/100 g DM). In contrast, the majority of fibre remained in the insoluble fraction but rates of degradation (k) approximately halved as grass matured. In vitro yield of VFA was similar for immature and mature minced ryegrass (after 12 hours VFA was equivalent to about 30% of DM), even though ammonia concentration declined to very low values for stem and mature grass. This suggests the rapid initial microbial growth was able to sustain a high level of DM degradation to VFA with mature grass. The summer pasture used for silage supplementation was of uncharacteristically good quality so the expected contrasts between maize, pasture, sulla (Hedysarum coronarium), lotus (Lotus corniculatus) and sulla/maize silage mixtures were less than expected. Milk responses to lotus silage supplements were greater than other silages (e.g.: 290 g milksolids from 54 MJ ME by lotus versus 110 g milksolids from about 50 MJ ME supplied by other silages). Pasture substitution was low (0.06 – 0.33). The Cornell Net Carbohydrate and Protein System (CNCPS) was chosen for evaluation of cow trial data because it uses feed degradation parameters as input variables to estimate nutrient supply. Model prediction of milk yield matched observed values when cows maintained liveweight. Milk yield was underestimated at low intakes and overestimated at high intakes because no allowance is made for nutrient partitioning between milk production and liveweight change.