Teses / dissertações sobre o tema "NMR methodology"
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Bayro, Marvin J. "Protein MAS NMR methodology and structural analysis of protein assemblies". Thesis, Massachusetts Institute of Technology, 2010. http://hdl.handle.net/1721.1/57800.
Texto completo da fonteVita. Cataloged from PDF version of thesis.
Includes bibliographical references.
Methodological developments and applications of solid-state magic-angle spinning nuclear magnetic resonance (MAS NMR) spectroscopy, with particular emphasis on the analysis of protein structure, are described in this thesis. MAS NMR studies of biomolecules ranging from model peptides and proteins in crystalline form to amyloid fibrils and whole bacterial organelles are reported. The methods presented include novel pulse sequences and optimized pulse sequence elements, experimental approaches designed for multiple-spin systems, a protocol for efficient sequential resonance assignment of proteins in the solid state, and techniques to determine the inter-molecular organization of amyloid fibrils formed by moderately sized proteins. Notably, an efficient dipolar recoupling technique, bandselective radio frequency-driven recoupling (BASE RFDR), is introduced and combined with alternating 13C-12C labeling to yield highly sensitive 13C-13C correlation spectra between distant nuclei in proteins. Various applications of the BASE RFDR scheme are presented, including protein resonance assignment, determination of tertiary structure of amyloid fibrils, and variable-temperature studies of protein dynamics. The main biological systems analyzed are amyloid fibrils formed by the SH3 domain of P13 kinase (P13-SH3) and intact gas vesicles from anabaena flos-aquae, for which atomic-level structural information was previously unavailable. P13-SH3 (86 residues) is a system thoroughly studied as a model of protein misfolding and amyloid formation by a natively globular protein. Gas vesicles are bacterial buoyancy organelles, with walls composed almost entirely by a single protein (GvpA, 70 residues), whose formation and structure constitute a highly intriguing biophysical problem. Nearly complete 13C and 'IN resonance assignments and the molecular conformations of the polypeptide backbones of both P13-SH3 and GvpA have been obtained via MAS NMR spectroscopy, enabling the proposal of models for the structure of these two protein assembly systems. In addition, the tertiary structure of P13-SH3 amyloid fibrils has been elucidated by the application of novel methodology introduced in this thesis. Finally, investigations regarding the effects of temperature and protein dynamics on MAS NMR experiments and biomolecular dynamic nuclear polarization studies are presented.
by Marvin J. Bayro.
Ph.D.
Pöppler, Ann-Christin. "Advanced NMR Methodology for the Investigation of Organometallic Compounds in Solution". Doctoral thesis, Niedersächsische Staats- und Universitätsbibliothek Göttingen, 2013. http://hdl.handle.net/11858/00-1735-0000-0001-BC26-5.
Texto completo da fonteNambiath, chandran Jima. "Development of NMR methodology for the analysis and simplification of complex mixtures". Thesis, Aix-Marseille, 2013. http://www.theses.fr/2013AIXM4306.
Texto completo da fonteThis thesis work deals with the analysis of natural and synthetic complex mixtures composed of small molecules using HRMAS NMR. In a first part, an integrated HRMAS-NMR based metabolomic analysis in combination with pattern recognition techniques (PCA and O-PLS-DA) has been applied for the diagnosis of indeterminate thyroid lesions and also studied the potential adverse biological effects of aluminium nanoparticles on pseudomonas brassicacearum. In a second part we investigated that chromatographic NMR using silica as the matrix support could provide a quick alternative and complement to LC for the characterization of complex mixtures. In addition, requirement for signal suppression in natural plant extract and aromatic hydrocarbons led to the development of a rapid and accurate method using molecularly imprinted polymers with excellent selectivity. The selectivity of Molecularly Imprinted polymers towards capturing a specific molecular target is exploited here to efficiently remove NMR signals
Nambiath, chandran Jima. "Development of NMR methodology for the analysis and simplification of complex mixtures". Electronic Thesis or Diss., Aix-Marseille, 2013. http://www.theses.fr/2013AIXM4306.
Texto completo da fonteThis thesis work deals with the analysis of natural and synthetic complex mixtures composed of small molecules using HRMAS NMR. In a first part, an integrated HRMAS-NMR based metabolomic analysis in combination with pattern recognition techniques (PCA and O-PLS-DA) has been applied for the diagnosis of indeterminate thyroid lesions and also studied the potential adverse biological effects of aluminium nanoparticles on pseudomonas brassicacearum. In a second part we investigated that chromatographic NMR using silica as the matrix support could provide a quick alternative and complement to LC for the characterization of complex mixtures. In addition, requirement for signal suppression in natural plant extract and aromatic hydrocarbons led to the development of a rapid and accurate method using molecularly imprinted polymers with excellent selectivity. The selectivity of Molecularly Imprinted polymers towards capturing a specific molecular target is exploited here to efficiently remove NMR signals
Lewandowski, Józef Romuald. "Methodology and applications of high resolution solid-state NMR to structure determination of proteins". Thesis, Massachusetts Institute of Technology, 2008. http://hdl.handle.net/1721.1/45640.
Texto completo da fonteThis electronic version was submitted by the student author. The certified thesis is available in the Institute Archives and Special Collections.
Vita.
Includes bibliographical references.
A number of methodological developments and applications of solid-state NMR for assignment and high resolution structure determination of microcrystalline proteins and amyloid fibrils are presented. Magic angle spinning spectroscopy on uniformly and selectively "C and '5N labeled samples is performed at magnetic fields from 11.7 to 21.1 T and spinning frequencies from 9 to 65 kHz.Dynamic Nuclear Polarization on nanocrystals of amyloidogenic peptide GNNQQNY is presented demonstrating that 'H-'H spin diffusion can efficiently transfer the enhanced polarization across the solute that is not in an intimate contact with the polarizing agent.An improved theoretical treatment of Rotational Resonance Width (R2W) experiments and its application to determination of precise 13C-13C distance is presented. A general theory of second averaging in modulation frame for designing solid-state NMR experiments is introduced and discussed in the context of two methods: Cosine Modulated Rotary Resonance (CMpRR) for performing a broadband double-quantum 13C-13C recoupling without the need for additional 'H decoupling and Cosine Modulated recoupling with Chemical Shift reintroduction (COMICS) that provides a general frequency selective method for measuring precise 13C-13C distances in uniformly labeled solids. Cosine Modulated Adiabatic Recoupling (CMAR) - an adiabatic extension of the CMpRR, that is particularly robust with respect to rf inhomogeneity, is also introduced. A number of applications CMpRR at 21.1 T to proteins with varying degrees of macroscopic order are presented. A second order Third Spin Assisted Recoupling (TSAR) mechanism is introduced and discussed in detail. The heteronuclear TSAR - Proton Assisted Insensitive Nuclei Cross-Polarization (PAIN-CP) and homonuclear Proton Assisted Recoupling (PAR) yield long distance 13C_1-N, 3C-_13C and 15N- 5N restraints in uniformly labeled systems with spinning frequencies up to 65 kHz that are used for protein structure calculation. Structure, dynamics and polymorphism of amyloidogenic peptide GNNQQNY from the yeast protein sup35p are investigated. Finally, PAIN-CP and '3C-13C PAR are used for high resolution de novo structure determination of 10.4 kDa Crh protein dimer.
by Józef Romuald Lewandowski.
Ph.D.
Kleks, Guy. "Development of NMR Methodology for Complex Mixture Analysis and Discovery of Antiplasmodial Marine Natural Products". Thesis, Griffith University, 2021. http://hdl.handle.net/10072/401350.
Texto completo da fonteThesis (PhD Doctorate)
Doctor of Philosophy (PhD)
School of Environment and Sc
Science, Environment, Engineering and Technology
Full Text
Linser, Rasmus Jan. "Development and application of new methodology for 1 H-detected MAS solid-state NMR on biomolecules". Doctoral thesis, Humboldt-Universität zu Berlin, Mathematisch-Naturwissenschaftliche Fakultät I, 2010. http://dx.doi.org/10.18452/16199.
Texto completo da fonteIn this work, novel solid-state NMR (Nuclear Magnetic Resonance) experiments are presented that imply direct detection of protons. The technique is based on extensive dilution of protons with deuterons in uniformly labelled, recombinantly expressed proteins and allows for solid-state NMR providing very narrow lines of all commonly accessible nuclei (protons, nitrogen, carbon) without high-power decoupling. In addition, methods are developed that yield a particularly high signal-to-noise through Paramagnetic Relaxation Enhancement (PRE). The accordingly prepared proteins are shown to be applicable for a significantly improved NMR-characterization by manifold new experiments for assignment and structure elucidation, in which techniques from solid-state and solution NMR are united. For the first time, also those regions in a protein can be accessed that undergo slow dynamics. The experiments are employed on the SH3-domain of alpha-spectrin, Alzheimer’s peptide Abeta1‑40, and the membrane protein Omp G.
Myers, Emma Paige. "Solid State ²H NMR Spectroscopy Analysis of Lipid-Protein Interactions in Response to Bilayer Deformations: Development of Methodology". Thesis, The University of Arizona, 2013. http://hdl.handle.net/10150/297723.
Texto completo da fonteGrohe, Kristof [Verfasser], e Rasmus [Akademischer Betreuer] Linser. "Development and application of novel NMR methodology for elucidation of protein structure and dynamics / Kristof Grohe ; Betreuer: Rasmus Linser". München : Universitätsbibliothek der Ludwig-Maximilians-Universität, 2020. http://d-nb.info/122384983X/34.
Texto completo da fontePöppler, Ann-Christin [Verfasser], Dietmar [Akademischer Betreuer] Stalke e Philipp [Akademischer Betreuer] Vana. "Advanced NMR Methodology for the Investigation of Organometallic Compounds in Solution / Ann-Christin Pöppler. Gutachter: Dietmar Stalke ; Philipp Vana. Betreuer: Dietmar Stalke". Göttingen : Niedersächsische Staats- und Universitätsbibliothek Göttingen, 2013. http://d-nb.info/1044870362/34.
Texto completo da fonteInce, Ridvan. "Étude du magnétisme moléculaire local par RMN des solides paramagnétiques à haute résolution". Electronic Thesis or Diss., Université de Lorraine, 2022. http://www.theses.fr/2022LORR0206.
Texto completo da fonteThe purpose of this work is to try and set up a new tool for the experimental determination of local magnetic susceptibility tensor. We have worked on an isotructural series of lanthanide oxalate [Ln2(C2O4)3, 9,5H2O] with Ln = La, Ce, Pr, Nd, using NMR of the paramagnetic solid in order to show thefeasability of this kind of studies by solid-state NMR.To determine the local magnetic susceptibility, we propose a semi-empirical approach based on a parameterized point dipole model for local magnetism. The model is based on the knowledge of the crystal structure and the empirical approximation of the point dipole for each paramagnetic center in the structure. The hyperfine dipole interaction is calculated for each NMR observable nucleus by summing the effect of each paramagnetic center on the NMR spectrum within the radius of convergence of our model. The resulting spectrum is then compared to the experimental data and the model parameters are optimized to obtain the best fit.The fact that this semi-empirical model is very fast to calculate opens a wide panel of accessible measurements through this method. For instance, we were able to perform a statistical analysis of each parameter of the local magnetic susceptibility tensor.We have compared our results with SQUID measurements of the macroscopic susceptibility and it showed a good agreement between the isotropic component obtained by both measurements.We explored several possibilities offered by this method in order to access the diamagnetic components of the chemical shift tensor by changing the temperature of our samples. We also tried to push ourstudies towards disordered systems for which diffraction methods have more difficulties to access local magnetic informations
Carneiro, Marta Verfasser], Christian [Akademischer Betreuer] [Griesinger, Jörg [Akademischer Betreuer] Enderlein, Tim [Akademischer Betreuer] Grüne, Kai [Akademischer Betreuer] Tittmann, Markus [Akademischer Betreuer] Zweckstetter e Bert de [Akademischer Betreuer] Groot. "Functional dynamics of the anti-HIV lectin OAA and NMR methodology for the study of protein dynamics / Marta Carneiro. Betreuer: Christian Griesinger. Gutachter: Jörg Enderlein ; Tim Grüne ; Kai Tittmann ; Markus Zweckstetter ; Bert De Groot". Göttingen : Niedersächsische Staats- und Universitätsbibliothek Göttingen, 2016. http://d-nb.info/108182042X/34.
Texto completo da fonteDomah, Darshan. "The NERV Methodology: Non-Functional Requirements Elicitation, Reasoning, and Validation in Agile Processes". NSUWorks, 2013. http://nsuworks.nova.edu/gscis_etd/137.
Texto completo da fonteBert, Emmanuel. "Dynamic urban origin-destination matrix estimation methodology /". Lausanne : EPFL, 2009. http://library.epfl.ch/theses/?nr=4417.
Texto completo da fonteLocher, Georg. "Bioprocess automation : equipment, methodology and benefits /". [S.l.] : [s.n.], 1991. http://e-collection.ethbib.ethz.ch/show?type=diss&nr=9594.
Texto completo da fonteMunk, Charles. "A methodology for designing and using a hardware system specification environment /". [S.l.] : [s.n.], 1995. http://library.epfl.ch/theses/?nr=1309.
Texto completo da fonteAlasonati, Enrica. "Development of a hyphenated analytical methodology for the fractionation and characterization of environmental colloids /". Lausanne : EPFL, 2009. http://library.epfl.ch/theses/?nr=4325.
Texto completo da fonteAvileis, Junior Laercio 1947. "Proposta de modelo de maturidade em gerenciamento de projetos conforme NBR ISO 21.500:2012". [s.n.], 2015. http://repositorio.unicamp.br/jspui/handle/REPOSIP/265827.
Texto completo da fonteTese (doutorado) - Universidade Estadual de Campinas, Faculdade de Engenharia Mecânica
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Resumo: Propõe-se, neste trabalho, a criação de um Modelo de Maturidade em Gerenciamento de Projetos, para a norma NBR ISO 21.500:2012, que possa ser aplicado na avaliação do desempenho do gerenciamento de projetos e contribuir para o sucesso do projeto. Com tal objetivo, desenvolveu-se um questionário que contempla 39 questões associadas aos processos da norma. O questionário foi, então, submetido às empresas que faziam o gerenciamento de seus projetos de forma organizada e às empresas que tinham, como produto final, projetos. O método de pesquisa adotado foi o de estudo de campo. Ou seja, por meio de uma pesquisa de natureza exploratória e descritiva, investigou-se a importância dos processos de gerenciamento de projetos. As respostas obtidas pelo questionário foram avaliadas a partir de métodos estatísticos, a saber, a Análise de Componentes Principais e a Análise Hierárquica de Processos. Criou-se, destarte, uma parametrização que permite a pontuação por questão e que avalia o nível do gerenciamento de projetos da organização. Tais valores e critérios, por fim, servirão como referência às empresas que desejam otimizar continuamente seus processos através de avaliações sistemáticas e relatórios de lições aprendidas
Abstract: This work proposes the production of a Project Management Maturity Model for the NBR ISO 21.500:2012 standard, to be used as a tool to evaluate the project management, and thus contributing to the success of the project. For that purpose, a questionnaire was developed which includes 39 questions related to the above mentioned standard. This questionnaire was then submitted to companies that have projects as final products, and to companies that undertake project management in a systematic way. The research method consisted of an exploratory and descriptive field study through e-survey, which investigated the importance of the processes of project management. The answers were evaluated using statistical methods, namely Principal Components Analysis and Analytic Hierarchy Process, which set the relative weights of each process. A parameterization was created to grant a score for each question, thus assessing the performance of project management of the organization. Hence the proposed model provides scores and criteria that will enable organizations to improve their processes continuously through systematic evaluations and collections of lessons learned
Doutorado
Materiais e Processos de Fabricação
Doutor em Engenharia Mecânica
Casi, Giulio. "Peptide bond forming reactions : from methodology to the construction of a semisynthetic enzyme /". Zürich : ETH, 2007. http://e-collection.ethbib.ethz.ch/show?type=diss&nr=17598.
Texto completo da fonteMarquezi, Marina Chiarelli. "Comparação de metodologias para a estimativa do número mais provável (NMP) de coliformes em amostras de água". Universidade de São Paulo, 2010. http://www.teses.usp.br/teses/disponiveis/11/11141/tde-21102010-160234/.
Texto completo da fonteIn almost all aquatic environments are found in various microorganisms, including some pathogenic to man, which route of transmission is the fecal-oral cycle. Because the water is the food most consumed by people, it is associated with several cases of diseases and risks to public health. Thus, control of water quality prior to consumption is essential, and to guarantee its quality must be made analysis seeking to indicate contamination by fecal material of origin. The group of bacteria most commonly used for the determination of fecal contamination is the total coliform group, which has as subgroup thermotolerant coliforms. The bacterium Escherichia coli is a member of the group thermotolerant coliform, and is most suitable for determination of fecal contamination by being present in large quantities in feces of humans and warmblooded animals. For the analysis of bacteria of the group coliform and E. coli there are several methods, including the conventional method Multiple Tube Fermentation (MTF), and the rapid methods Colilert and Colitag. This study aimed to compare these three methods for analysis in water samples from different points with different contamination (supply, fountain and river) and compare the count found for thermotolerant coliform and E. coli by the method FTM. The methods Colilert, Colitag and FTM were equivalent in the analysis of total coliform in water samples from the fountain and supply, and the analysis of E. coli in samples of supply, fountain and river. For analysis of total coliform in water samples from the river, FTM method showes a significant difference compared to the methods Colilert and Colitag. Both rapid methods did not differ. The difference between these methods in these tests may be due to the presence of Aeromonas in the samples, which may have generated false-positive results in rapid methods, overestimating the total coliform count; or the largest capacity of rapid methods in recovering injured cells of coliforms, detecting a larger amount of these bacteria. Comparing the scores found for thermotolerant coliform and E. coli in the same samples was found a significant difference. This result is explained by a thermotolerant coliform group include bacteria not exclusively fecal origin, such as Klebsiella and Enterobacter, which may overestimate the faecal contamination of the sample. Since E. coli is exclusively fecal origin, being more suitable for such analysis. So, as rapid methods showed to be equivalent to the conventional method studied, one should prefer the use of rapid methods that give results in 24 hours, indicating the water quality in time to prevent its use in the population. In choosing between coliform thermotolerant bacteria and E. coli for the indication of fecal contamination, it is preferable to use E. coli.
Semeniuk, Trudi Ann. "A new descriptive methodology and study of polymineralic fault rocks from reactivated shear zones of the Ivrea Zone, Northern Italy /". [Zürich], 2003. http://e-collection.ethbib.ethz.ch/show?type=diss&nr=15247.
Texto completo da fontePereira, Iran Cosme. "Modelo para integração dos sistemas de gestão da qualidade, meio ambiente, segurança, saúde ocupacional e responsabilidade social em uma empresa têxtil". Universidade Federal de Pernambuco, 2011. https://repositorio.ufpe.br/handle/123456789/5969.
Texto completo da fonteNo atual cenário competitivo, marcado pelo crescente nível de exigência de mercado, aumento do nível de eficiência das máquinas e redução nos custos de fabricação, as indústrias precisam gerenciar seus processos produtivos e ao mesmo tempo preservar e recuperar o meio ambiente. Essas crescentes exigências promovem uma maior consciência dos gestores, que passam a compreender que o investimento em práticas sustentáveis está relacionado à sobrevivência da empresa. Essa contínua preocupação das organizações com esse panorama demonstra essa necessidade de melhoria principalmente do ramo têxtil, que ainda atua nos seus negócios com modelos administrativos ultrapassados, atuando de forma reativa e apresentando uma necessidade de incorporar aos seus modelos produtivos e administrativos uma gestão mais eficaz dos seus processos. Neste sentido, como forma de estabelecimento de uma cultura voltada às práticas de gestão sistematizadas, é proposto nesta dissertação, um modelo para a integração dos sistemas de gestão da qualidade, meio ambiente, segurança, saúde ocupacional e responsabilidade social. Para isto, foi adotada a Soft Systems Methodology, proposta por Checkland. Os resultados obtidos permitiram efetuar o diagnóstico obtido a partir da aplicação da análise do campo de forças para a implantação do Sistema Integrado de Gestão proposto, deixando claro quais as forças restritivas e impulsionadoras ao sistema, devem ser equilibradas antes de iniciar a sua implantação
Silva, Osvaldo Cirilo da. "Processo de fabricação de comprimidos de lamivudina e zidovudina (150+300mg): avaliação retrospectiva da variabilidade e desenvolvimento de metodologia analítica por espectroscopia no infravermelho próximo com transformada de Fourier (FT-NIR) aplicada a avaliação da homogeneidade da mistura de pós". Universidade de São Paulo, 2018. http://www.teses.usp.br/teses/disponiveis/9/9139/tde-29012019-155121/.
Texto completo da fonteThe use of statistical tools in the life cycle of a pharmaceutical product allows verifying and controlling the process aiming at its continuous improvement. In the present study, the stability and statistical capacity of the lamivudine coated tablets 3TC and zidovudine AZT (150 + 300 mg) manufactured by the Chopin Tavares de Lima Foundation (FURP) were evaluated. This drug, distributed free of charge by the Ministry of Health\'s DST/AIDS program, is manufactured by direct compression, a rapid process that allows the future implementation of Process Analytical Technology (PAT). In Chapter I, a retrospective evaluation of the variability of critical quality attributes of 529 batches of tablets manufactured was carried out, such attributes being: mean weight, unit dose uniformity and % m/v of dissolved drug substances, before and after coating. The objective was to identify possible special causes of variability of the processes that allow continuous improvements. In Chapter II an analytical methodology was developed employing the near infrared spectroscopy with Fourier transform for the evaluation of the homogeneity of the powder mixture. In this study, samples of mixtures of the drugs lamivudine 3TC and zidovudine AZT and excipient mixture were analyzed, using as reference method the HPLC, for the quantification of these two drugs. In Chapter I, the evaluation of the process for the mean weight revealed the need to investigate the special cause of variability, as evidenced by the charts. The results of the year 2015 indicated the need for centralization and process consistency, with a reduction in the probability of failure. The control charts for unit dose uniformity, in the year 2013, revealed less process variability. However, in that year, the statistical analysis for dissolution revealed a decentralized process with no consistency, with greater evidence for the 3TC drug that showed lower performance, Cpk<1.0. The evaluation of the stability and capacity of the lamivudine + zidovudine tablet manufacturing process (150 + 300 mg) in the period from 2012 to 2015 allowed a better understanding of its sources of variation. It was possible to detect and determine the degree of this variation and its impact on the process and the critical quality attributes of the product with evident opportunities to improve the process, reducing risks for the patient. In Chapter II, in the development of the method, the validation revealed that the lowest values of BIAS were observed for 3TC, 0.000116 and 0.0021, respectively for cross validation and validation. BIAS values close to zero indicated a reduced percentage of variability of the method. The present study demonstrated the feasibility of using the model developed for the quantification of 3TC and AZT by FT-NIR after adjustments that contribute to the elevation of R, R2 and RPD to acceptable values. RPD values above 5.0 that allow the use of the model for use in quality control.
Arya, Neeru. "Development of Novel NMR Methodology and Investigation of Challenging Chemical Problems". Thesis, 2020. https://etd.iisc.ac.in/handle/2005/4688.
Texto completo da fonteParish, David Michael. "Development and application of methodology for rapid NMR data collection and protein structure determination". 2008. http://proquest.umi.com/pqdweb?did=1542146751&sid=6&Fmt=2&clientId=39334&RQT=309&VName=PQD.
Texto completo da fonteTitle from PDF title page (viewed on Dec. 4, 2008) Available through UMI ProQuest Digital Dissertations. Thesis adviser: Szyperski, Thomas A. Includes bibliographical references.
Carneiro, Marta. "Functional dynamics of the anti-HIV lectin OAA and NMR methodology for the study of protein dynamics". Doctoral thesis, 2015. http://hdl.handle.net/11858/00-1735-0000-0028-86A9-F.
Texto completo da fonteLinser, Rasmus Jan [Verfasser]. "Development and application of new methodology for 1H-detected MAS solid-state NMR on biomolecules / Rasmus Jan Linser". 2010. http://d-nb.info/101022221X/34.
Texto completo da fonte(7552283), L. Liu. "New syntheses of protoberberines: Cycloaddition and carbanionic methodology leading to the production of highly enantioselective products". Thesis, 1997. https://figshare.com/articles/thesis/New_syntheses_of_Protoberberines_Cycloaddition_and_carbanionic_methodology_leading_to_the_production_of_highly_enantioselective_products/13425983.
Texto completo da fonteWilliams, Justin A. "Biophysical studies of cholesterol in unsaturated phospholipid model membranes". Thesis, 2013. http://hdl.handle.net/1805/5067.
Texto completo da fonteCellular membranes contain a staggering diversity of lipids. The lipids are heterogeneously distr ibuted to create regions, or domains, whose physical properties differ from the bulk membrane and play an essential role in modulating the function of resident proteins. Many basic questions pertaining to the formation of these lateral assemblies remain. T his research employs model membranes of well - defined composition to focus on the potential role of polyunsaturated fatty acids (PUFAs) and their interaction with cholesterol (chol) in restructuring the membrane environment. Omega - 3 (n - 3) PUFAs are the main bioactive components of fish oil, whose consumption alleviates a variety of health problems by a molecular mechanism that is unclear. We hypothesize that the incorporation of PUFAs into membrane lipids and the effect they have on molecular organization may be, in part, responsible. Chol is a major constituent in the plasma membrane of mammals. It determines the arrangement and collective properties of neighboring lipids, driving the formation of domains via differential affinity for different lipids . T he m olecular organization of 1 -[ 2 H 31 ]palmitoyl -2- eicosapentaenoylphosphatidylcholine (PEPC - d 31 ) and 1 -[ 2 H 31 ]palmitoyl -2- docosahexaenoylphosphatidylcholine (PDPC -d 31 ) in membran es with sphingomyelin (SM) and chol (1:1:1 mol) was compared by solid - state 2 H NMR spectroscopy. Eicosapentaenoic (EPA) and docosahexaenoic (DHA) acids are the two major n - 3 PUFAs found in fish oil, while PEPC -d 31 and PDPC -d 31 are phospholipids containing the respective PUFAs at the sn - 2 position and a perdeuterated palmitic acid a t the sn - 1 position . Analysis of s pectra recorded as a function of temperature indicate s that in both cases, formation of PUFA - rich (less ordered) and SM - rich (more ordered) domains occurred. A surprisingly substantial proportion of PUFA was found to infil trate the more ordered domain. There was almost twice as much DHA (65%) as EPA (30%) . The implication is that n - 3 PUFA s can incorporate into lipid rafts, which are domains enriched in SM and chol in the plasma membrane, and potentially disrupt the activity of signaling proteins that reside therein. DHA, furthermore, may be the more potent component of fish oil. PUFA - chol interactions were also examined through affinity measurements. A novel method utilizing electron paramagnetic resonance (EPR) was develope d, to monitor the partitioning of a spin - labeled analog of chol , 3β - doxyl - 5α - cholestane (chlstn), between large unilamellar vesicles (LUVs) and met hyl - β - cyclodextrin (mβCD). The EPR spectra for chlstn in the two environments are distinguishable due to the substantial differences in tumbling rates , allowing the population distribution ratio to be determined by spectral simulation. Advantages of this approach include speed of implementation and a vo idance of potential artifact s associated with physical separation of LUV and mβCD . Additionally, in a check of the method, t he relative partition coefficients between lipids measured for the spin label analog agree with values obtained for chol by isothermal titration calorimetry (ITC). Results from LUV with different composition confirmed a hierarchy of decreased sterol affinity for phospholipids with increasing acyl chain unsaturation , PDPC possessing half the affinity of the corresponding monounsaturated phospholipid. Taken together, the results of these studies on model membranes demonstrate the potential for PUFA - driven alteration of the architecture of biomembranes, a mechanism through which human health may be impacted.
Long, Jeremy. "Simulating visual systems using NPR techniques: methodology, framework, and case studies". Thesis, 2012. http://hdl.handle.net/1828/3887.
Texto completo da fonteGraduate
Klein, Markus Sebastian. ""Dies ist deutsche Literatur, nur ohne Komma und Punkt" - Entwicklung, Methodologie und Analyse von Deutschrap". Thesis, 2011. http://hdl.handle.net/10012/6228.
Texto completo da fonte"Experiência of a software methodology in the rio". Tese, MAXWELL, 1993. http://www.maxwell.lambda.ele.puc-rio.br/cgi-bin/db2www/PRG_0991.D2W/SHOW?Cont=8788:pt&Mat=&Sys=&Nr=&Fun=&CdLinPrg=pt.
Texto completo da fonte"Methodology systems for peak capacity expansion of interconnected hydroelectric". Tese, MAXWELL, 1991. http://www.maxwell.lambda.ele.puc-rio.br/cgi-bin/db2www/PRG_0991.D2W/SHOW?Cont=9276:pt&Mat=&Sys=&Nr=&Fun=&CdLinPrg=pt.
Texto completo da fonte"Methodology for implementation and operation planning of mobile cellular systems". Tese, MAXWELL, 2001. http://www.maxwell.lambda.ele.puc-rio.br/cgi-bin/db2www/PRG_0991.D2W/SHOW?Cont=2158:pt&Mat=&Sys=&Nr=&Fun=&CdLinPrg=pt.
Texto completo da fonte"Assessing undiscovered oil and gas resources: methodology and elicitation of subjective information". Tese, MAXWELL, 2006. http://www.maxwell.lambda.ele.puc-rio.br/cgi-bin/db2www/PRG_0991.D2W/SHOW?Cont=9219:pt&Mat=&Sys=&Nr=&Fun=&CdLinPrg=pt.
Texto completo da fonte"An alternative methodology for the seismic structural analysis of thermo-nuclear power plants". Tese, MAXWELL, 1999. http://www.maxwell.lambda.ele.puc-rio.br/cgi-bin/db2www/PRG_0991.D2W/SHOW?Cont=1265:pt&Mat=&Sys=&Nr=&Fun=&CdLinPrg=pt.
Texto completo da fonte"Estimation of the family consumption for national accounts - the proposition of a methodology". Tese, MAXWELL, 2001. http://www.maxwell.lambda.ele.puc-rio.br/cgi-bin/db2www/PRG_0991.D2W/SHOW?Cont=1728:pt&Mat=&Sys=&Nr=&Fun=&CdLinPrg=pt.
Texto completo da fonte"Behavioral finance and box and jenkins methodology: an application on the brazilian market". Tese, MAXWELL, 2006. http://www.maxwell.lambda.ele.puc-rio.br/cgi-bin/db2www/PRG_0991.D2W/SHOW?Cont=8764:pt&Mat=&Sys=&Nr=&Fun=&CdLinPrg=pt.
Texto completo da fonte"Modeling and forecast of the recoverable oil volume: methodology and application in brazilian basins". Tese, MAXWELL, 2006. http://www.maxwell.lambda.ele.puc-rio.br/cgi-bin/db2www/PRG_0991.D2W/SHOW?Cont=9628:pt&Mat=&Sys=&Nr=&Fun=&CdLinPrg=pt.
Texto completo da fonte"Economic regulation in the brazilian electric power supply sector: a methodology for defining production efficiency frontier and estimating the x-factor". Tese, MAXWELL, 2004. http://www.maxwell.lambda.ele.puc-rio.br/cgi-bin/db2www/PRG_0991.D2W/SHOW?Cont=5566:pt&Mat=&Sys=&Nr=&Fun=&CdLinPrg=pt.
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