Bibliografias temáticas / Natural bioink

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Literatura científica selecionada sobre o tema "Natural bioink"

Autor: Grafiati
Publicado: 29 de março de 2025

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Artigos de revistas sobre o assunto "Natural bioink"

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Willson, Kelsey, Anthony Atala e James J. Yoo. "Bioprinting Au Natural: The Biologics of Bioinks". Biomolecules 11, n.º 11 (28 de outubro de 2021): 1593. http://dx.doi.org/10.3390/biom11111593.

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The development of appropriate bioinks is a complex task, dependent on the mechanical and biochemical requirements of the final construct and the type of printer used for fabrication. The two most common tissue printers are micro-extrusion and digital light projection printers. Here we briefly discuss the required characteristics of a bioink for each of these printing processes. However, physical printing is only a short window in the lifespan of a printed construct—the system must support and facilitate cellular development after it is printed. To that end, we provide a broad overview of some of the biological molecules currently used as bioinks. Each molecule has advantages for specific tissues/cells, and potential disadvantages are discussed, along with examples of their current use in the field. Notably, it is stressed that active researchers are trending towards the use of composite bioinks. Utilizing the strengths from multiple materials is highlighted as a key component of bioink development.
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Zhe, Man, Xinyu Wu, Peiyun Yu, Jiawei Xu, Ming Liu, Guang Yang, Zhou Xiang, Fei Xing e Ulrike Ritz. "Recent Advances in Decellularized Extracellular Matrix-Based Bioinks for 3D Bioprinting in Tissue Engineering". Materials 16, n.º 8 (18 de abril de 2023): 3197. http://dx.doi.org/10.3390/ma16083197.

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In recent years, three-dimensional (3D) bioprinting has been widely utilized as a novel manufacturing technique by more and more researchers to construct various tissue substitutes with complex architectures and geometries. Different biomaterials, including natural and synthetic materials, have been manufactured into bioinks for tissue regeneration using 3D bioprinting. Among the natural biomaterials derived from various natural tissues or organs, the decellularized extracellular matrix (dECM) has a complex internal structure and a variety of bioactive factors that provide mechanistic, biophysical, and biochemical signals for tissue regeneration and remodeling. In recent years, more and more researchers have been developing the dECM as a novel bioink for the construction of tissue substitutes. Compared with other bioinks, the various ECM components in dECM-based bioink can regulate cellular functions, modulate the tissue regeneration process, and adjust tissue remodeling. Therefore, we conducted this review to discuss the current status of and perspectives on dECM-based bioinks for bioprinting in tissue engineering. In addition, the various bioprinting techniques and decellularization methods were also discussed in this study.
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Goklany, Sheba. "Conductive Nanomaterials used in Bioinks for 3D Bioprinting". Nano LIFE 11, n.º 02 (junho de 2021): 2130005. http://dx.doi.org/10.1142/s1793984421300053.

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Biofabrication for tissue engineering and regenerative medicine is a rapidly evolving field that incorporates bioprinting or bioassembly for the development of biologically functional products with structural organization using cells, bioactive molecules, and biomaterials. Bioprinting is a biofabrication technology that utilizes biomaterials, living cells, and supporting materials, called bioink, to generate three-dimensional tissue constructs. Bioprinting offers several advantages over traditional scaffolding and microengineering methods such as precise architecture control, high reproducibility, and versatility. The ideal bioink should possess appropriate structural, mechanical, gelation, rheological, chemical, biological, degradation, and biomimetic properties for the desired application of the final product. Several natural and synthetic bioinks have been developed and this review has focused on conductive nanomaterials that have been used in combination with hydrogel materials for bioink synthesis.
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Delkash, Yasaman, Maxence Gouin, Tanguy Rimbeault, Fatemeh Mohabatpour, Petros Papagerakis, Sean Maw e Xiongbiao Chen. "Bioprinting and In Vitro Characterization of an Eggwhite-Based Cell-Laden Patch for Endothelialized Tissue Engineering Applications". Journal of Functional Biomaterials 12, n.º 3 (11 de agosto de 2021): 45. http://dx.doi.org/10.3390/jfb12030045.

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Three-dimensional (3D) bioprinting is an emerging fabrication technique to create 3D constructs with living cells. Notably, bioprinting bioinks are limited due to the mechanical weakness of natural biomaterials and the low bioactivity of synthetic peers. This paper presents the development of a natural bioink from chicken eggwhite and sodium alginate for bioprinting cell-laden patches to be used in endothelialized tissue engineering applications. Eggwhite was utilized for enhanced biological properties, while sodium alginate was used to improve bioink printability. The rheological properties of bioinks with varying amounts of sodium alginate were examined with the results illustrating that 2.0–3.0% (w/v) sodium alginate was suitable for printing patch constructs. The printed patches were then characterized mechanically and biologically, and the results showed that the printed patches exhibited elastic moduli close to that of natural heart tissue (20–27 kPa) and more than 94% of the vascular endothelial cells survived in the examination period of one week post 3D bioprinting. Our research also illustrated the printed patches appropriate water uptake ability (>1800%).
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Zhang, Chun-Yang, Chao-Ping Fu, Xiong-Ya Li, Xiao-Chang Lu, Long-Ge Hu, Ranjith Kumar Kankala, Shi-Bin Wang e Ai-Zheng Chen. "Three-Dimensional Bioprinting of Decellularized Extracellular Matrix-Based Bioinks for Tissue Engineering". Molecules 27, n.º 11 (26 de maio de 2022): 3442. http://dx.doi.org/10.3390/molecules27113442.

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Three-dimensional (3D) bioprinting is one of the most promising additive manufacturing technologies for fabricating various biomimetic architectures of tissues and organs. In this context, the bioink, a critical element for biofabrication, is a mixture of biomaterials and living cells used in 3D printing to create cell-laden structures. Recently, decellularized extracellular matrix (dECM)-based bioinks derived from natural tissues have garnered enormous attention from researchers due to their unique and complex biochemical properties. This review initially presents the details of the natural ECM and its role in cell growth and metabolism. Further, we briefly emphasize the commonly used decellularization treatment procedures and subsequent evaluations for the quality control of the dECM. In addition, we summarize some of the common bioink preparation strategies, the 3D bioprinting approaches, and the applicability of 3D-printed dECM bioinks to tissue engineering. Finally, we present some of the challenges in this field and the prospects for future development.
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Chen, Yan, Yingge Zhou e Chi Wang. "Investigation of Collagen-Incorporated Sodium Alginate Bioprinting Hydrogel for Tissue Engineering". Journal of Composites Science 6, n.º 8 (4 de agosto de 2022): 227. http://dx.doi.org/10.3390/jcs6080227.

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Tissue engineering is a promising area that is aimed at tissue regeneration and wound repair. Sodium alginate (SA) has been widely used as one of the most biocompatible materials for tissue engineering. The cost-efficiency and rapid gel ability made SA attractive in would healing and regeneration area. To improve printability and elasticity, many hydrogel-based bioinks were developed by mixing SA with other natural or synthetic polymers. In this paper, composite SA/COL bioink was used for the bioprinting of artificial cartilage tissue mimicries. The results showed that the concentration of both SA and COL has significant effects on filament diameter and merging. A higher concentration of the bioink solution led to better printing fidelity and less deformation. Overall, a higher SA concentration and a lower COL concentration contributed to a lower shrinkage ratio after crosslinking. In summary, the SA/COL composite bioink has favorable rheological properties and this study provided material composition optimization for future bioprinting of engineered tissues.
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Tolmacheva, Nelli, Amitava Bhattacharyya e Insup Noh. "Calcium Phosphate Biomaterials for 3D Bioprinting in Bone Tissue Engineering". Biomimetics 9, n.º 2 (6 de fevereiro de 2024): 95. http://dx.doi.org/10.3390/biomimetics9020095.

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Three-dimensional bioprinting is a promising technology for bone tissue engineering. However, most hydrogel bioinks lack the mechanical and post-printing fidelity properties suitable for such hard tissue regeneration. To overcome these weak properties, calcium phosphates can be employed in a bioink to compensate for the lack of certain characteristics. Further, the extracellular matrix of natural bone contains this mineral, resulting in its structural robustness. Thus, calcium phosphates are necessary components of bioink for bone tissue engineering. This review paper examines different recently explored calcium phosphates, as a component of potential bioinks, for the biological, mechanical and structural properties required of 3D bioprinted scaffolds, exploring their distinctive properties that render them favorable biomaterials for bone tissue engineering. The discussion encompasses recent applications and adaptations of 3D-printed scaffolds built with calcium phosphates, delving into the scientific reasons behind the prevalence of certain types of calcium phosphates over others. Additionally, this paper elucidates their interactions with polymer hydrogels for 3D bioprinting applications. Overall, the current status of calcium phosphate/hydrogel bioinks for 3D bioprinting in bone tissue engineering has been investigated.
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Sanz-Fraile, Héctor, Carolina Herranz-Diez, Anna Ulldemolins, Bryan Falcones, Isaac Almendros, Núria Gavara, Raimon Sunyer, Ramon Farré e Jorge Otero. "Characterization of Bioinks Prepared via Gelifying Extracellular Matrix from Decellularized Porcine Myocardia". Gels 9, n.º 9 (13 de setembro de 2023): 745. http://dx.doi.org/10.3390/gels9090745.

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Since the emergence of 3D bioprinting technology, both synthetic and natural materials have been used to develop bioinks for producing cell-laden cardiac grafts. To this end, extracellular-matrix (ECM)-derived hydrogels can be used to develop scaffolds that closely mimic the complex 3D environments for cell culture. This study presents a novel cardiac bioink based on hydrogels exclusively derived from decellularized porcine myocardium loaded with human-bone-marrow-derived mesenchymal stromal cells. Hence, the hydrogel can be used to develop cell-laden cardiac patches without the need to add other biomaterials or use additional crosslinkers. The scaffold ultrastructure and mechanical properties of the bioink were characterized to optimize its production, specifically focusing on the matrix enzymatic digestion time. The cells were cultured in 3D within the developed hydrogels to assess their response. The results indicate that the hydrogels fostered inter-cell and cell-matrix crosstalk after 1 week of culture. In conclusion, the bioink developed and presented in this study holds great potential for developing cell-laden customized patches for cardiac repair.
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Lee, Juo, Sungmin Lee, Jae Woon Lim, Iksong Byun, Kyoung-Je Jang, Jin-Woo Kim, Jong Hoon Chung, Jungsil Kim e Hoon Seonwoo. "Development of Plum Seed-Derived Carboxymethylcellulose Bioink for 3D Bioprinting". Polymers 15, n.º 23 (21 de novembro de 2023): 4473. http://dx.doi.org/10.3390/polym15234473.

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Three-dimensional bioprinting represents an innovative platform for fabricating intricate, three-dimensional (3D) tissue structures that closely resemble natural tissues. The development of hybrid bioinks is an actionable strategy for integrating desirable characteristics of components. In this study, cellulose recovered from plum seed was processed to synthesize carboxymethyl cellulose (CMC) for 3D bioprinting. The plum seeds were initially subjected to α-cellulose recovery, followed by the synthesis and characterization of plum seed-derived carboxymethyl cellulose (PCMC). Then, hybrid bioinks composed of PCMC and sodium alginate were fabricated, and their suitability for extrusion-based bioprinting was explored. The PCMC bioinks exhibit a remarkable shear-thinning property, enabling effortless extrusion through the nozzle and maintaining excellent initial shape fidelity. This bioink was then used to print muscle-mimetic 3D structures containing C2C12 cells. Subsequently, the cytotoxicity of PCMC was evaluated at different concentrations to determine the maximum acceptable concentration. As a result, cytotoxicity was not observed in hydrogels containing a suitable concentration of PCMC. Cell viability was also evaluated after printing PCMC-containing bioinks, and it was observed that the bioprinting process caused minimal damage to the cells. This suggests that PCMC/alginate hybrid bioink can be used as a very attractive material for bioprinting applications.
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Gao, Qiqi, Byoung-Soo Kim e Ge Gao. "Advanced Strategies for 3D Bioprinting of Tissue and Organ Analogs Using Alginate Hydrogel Bioinks". Marine Drugs 19, n.º 12 (15 de dezembro de 2021): 708. http://dx.doi.org/10.3390/md19120708.

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Alginate is a natural polysaccharide that typically originates from various species of algae. Due to its low cost, good biocompatibility, and rapid ionic gelation, the alginate hydrogel has become a good option of bioink source for 3D bioprinting. However, the lack of cell adhesive moieties, erratic biodegradability, and poor printability are the critical limitations of alginate hydrogel bioink. This review discusses the pivotal properties of alginate hydrogel as a bioink for 3D bioprinting technologies. Afterward, a variety of advanced material formulations and biofabrication strategies that have recently been developed to overcome the drawbacks of alginate hydrogel bioink will be focused on. In addition, the applications of these advanced solutions for 3D bioprinting of tissue/organ mimicries such as regenerative implants and in vitro tissue models using alginate-based bioink will be systematically summarized.
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Mais fontes

Teses / dissertações sobre o assunto "Natural bioink"

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Abou, Nassif Lea. "Développement de bioencres naturelles enrichies par les composants de la gelée de Wharton pour la bioimpression 3D". Electronic Thesis or Diss., Reims, 2024. http://www.theses.fr/2024REIMS041.

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La bioimpression 3D est une technologie prometteuse pour la médecine régénératrice, permettant de concevoir des structures biomimétiques à l’aide de bioencres. Cette thèse se concentre sur le développement de bioencre(s) bioactive(s), enrichie(s) par des composants de la gelée de Wharton (GW). L’hydrogel formé à partir des composants de la GW, n’étant pas imprimable, a été combiné à des polymères naturels, tels que l’alginate et la gélatine, créant une bioencre thermosensible. Les paramètres d’impression, notamment la température et la pression, ont été optimisés pour assurer une meilleure précision.Les matrices imprimées à partir de la formulation d’alginate/gélatine type B avec ou sans hydrogel de la GW décellularisée, réticulées par du chlorure de calcium et de la transglutaminase (TG), constituent un support approprié pour les cellules, mais l’ajout de la GW n'a pas stimulé le recrutement et la prolifération des cellules souches mésenchymateuses (CSM) ni des fibroblastes. En raison du risque potentiel d'immunogénicité de l'alginate et de son effet négatif sur le comportement des cellules encapsulées, une seconde formulation a été développée. Elle était à base de gélatine de type A, plus réactive à la TG. L’impression des fibroblastes dans cette nouvelle bioencre la gélatine A a montré une bonne viabilité après 21 jours, en revanche la présence de l’hydrogel GW n’a pas été bénéfique pour les fibroblastes. L’ajout de milieu conditionné issu des CSM de la GW a, en revanche, augmenté la bioactivité de la bio-encre à base de gélatine type A
3D bioprinting is a promising technology for regenerative medicine, enabling the creation of biomimetic structures using specific bioinks. This thesis focuses on the development of bioactive bioinks, enriched with components from Wharton's Jelly (WJ) matrix. The WJ hydrogel, not being printable on its own, was combined with natural polymers such as alginate and gelatin to create a thermosensitive bioink. The printing parameters, especially temperature and pressure, were optimized to ensure better precision.Printed constructs from the alginate/type B gelatin formulation with or without WJ hydrogel, crosslinked with calcium chloride and transglutaminase (TG), provided an appropriate support for cells. However, the presence of WJ did not stimulate the recruitment or proliferation of mesenchymal stem cells (MSCs) or fibroblasts. Due to the potential immunogenicity of alginate and its negative impact on the behavior of printed cells, it was removed. The newest formulation was based on type A gelatin, more sensitive to TG activity. The printed fibroblasts in type A gelatin showed good viability after 21 days, but the addition of WJ hydrogel did not support cell viability after bioprinting. However, the addition of conditioned medium from WJ-derived MSCs enhanced the bioactivity of type A gelatine bioink
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Rhönnstad, Sofie. "Biotinylation and high affinity avidin capture as a strategy for LC-MS based metabolomics". Thesis, Linköping University, Department of Physics, Chemistry and Biology, 2010. http://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-56771.

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Metabolites, small endogenous molecules existing in every living cell, tissue or organism, play a vital role for maintaining life. The collective group of all metabolites, the metabolome, is a consequence of the biochemistry and biochemical pathways that a cell or tissue uses to promote survival. Analysis of the metabolome can be done to reveal changes of specific metabolites which can be a manifestation, a reason or a consequence of for example a disease. The physical chemical diversity amongst these components is tremendous and it poses a large analytical challenge to measure and quantify all of them. Targeting sub groups of the meta­bolome such as specific functional classes has shown potential for increasing metabolite coverage. Group selective labeling with biotin-tags followed by high affinity avidin capture is a well established purification strategy for protein purification.

The purpose with this project is to explore if it is possible to transfer the avidin biotin approach to metabolomics and use this method for small mole­cules purification. Specifically, this investigation aims to see if it is achievable to make a bio­tinylation of specific functional groups, to increase the sensitivity through reduction of sample complexity in liquid chromatography mass spectrometry metabolomics analyses after high affinity avidin capture. By purifying the analyte of interest and thereby reducing the sample complexity there will be a reduction in ion suppression. The aim is to increase the analytical sensitivity through a reduction in ion suppression during liquid chromatography mass spectrometry analysis.

Delimitations have been done to only investigate the possibility to obtain a biotinylation of primary amines and amides. As model compounds phenylalanine, spermi­dine, histamine and nicotinamide have been selected.

The result from this study indicates that it is possible to increase metabolite coverage through biotin labeling followed by high affinity avidin capture. It is a gain in analytical sensitivity of selected model compounds when comparing biotinylation strategy with a control non­biotinylation approach in a complex sample. A broader study of additional model compounds and a method development of this strategy are necessary to optimize a potential future method.

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Šáchová, Irena. "Bionika v architektuře (strom jako inspirační zdroj)". Doctoral thesis, Vysoké učení technické v Brně. Fakulta architektury, 2012. http://www.nusl.cz/ntk/nusl-233238.

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The work deals with nature prefigurations, which can be used in architecture. The first part is concerned with the bionics in architecture in general. There are introduced all available pieces of knowledge falling within this branch. The second part is focused on the nature prefiguration – tree. The tree was analyzed from different points of view. Suitable principles, which were found, were transformed to the field of architecture. On the basis of children's drawings the changing approach to “tree as a home” during adolescence was charted. The work can serve as a comprehensive supporting document for study of bionics for students of architecture and architects and also for the general public. The work should point out that nature is our great standard for any activity.
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Marcinonytė, Sigita. "Bioniškos architektūros ypatumai: konstrukcijos, ornamentika, ryšys su aplinka". Master's thesis, Lithuanian Academic Libraries Network (LABT), 2009. http://vddb.library.lt/obj/LT-eLABa-0001:E.02~2009~D_20090713_142527-19734.

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Baigiamajame magistro darbe „Bioniškos architektūros ypatumai: konstrukcijos, ornamentika, ryšys su aplinka“ architektūra nagrinėjama bionišku aspektu. Bioniškos architektūros nagrinėjimo gaires nustato bioniškos architektūros apibrėžimas, pasak kurio bioniška architektūra – tai architektūra, grindžiama gyvosios gamtos formomis ir organizmų sandaros principais. Iš jo matyti, kad tiek gamtinių struktūrų taikymas statinių konstruktyvui, tiek gamtinių formų vaizdavimas architektūroje daro tuos statinius bioniškus. Kita vertus, harmoningas statinio įkomponavimas į jį supančią aplinką, netaikant anksčiau paminėtų būdų, o naudojant kitas priemones, taip pat gali būti organiškas. Taigi architektūriniai statiniai, kurių forma išsilieja iš jų paskirties ir konkrečių gamtinių sąlygų, – kaip tai įprastai vyksta gamtinės kilmės organizmuose, – šiame darbe taip pat įtraukiami į bioniškos architektūros apibrėžimą. Visiems trims bioniškos architektūros išraiškos būdams atskleisti parenkami jiems atstovaujantys architektai. Jų suprojektuoti statiniai naudojami tolimesnei analizei, ieškoma panašumų ir skirtumų tarp skirtingų bioniškos architektūros atstovų. Baigiamojo darbo tikslas yra įrodyti, kad taikant skirtingas išraiškos priemones galima pasiekti to paties pobūdžio rezultatą, šiuo atveju, bionišką architektūrą.
The final post-graduate work focuses on three different aspects of bionic architecture –i.e. construction, ornamentation and smooth connection with environment. These guidelines are based on definition of bionic architecture. It says that bionic building is the one projected whether under natural decorative motifs or natural structures. However, harmonic composition of the building and the surrounding landscape using different expressions also creates an organic look. Thus the whole work is based on the analysis of the basic features of the bionic buildings. Those distinctive and common features are analyzed and compared together to find the essential ones that reflect the bionic connection. The main cause is to seek and to prove through the research of similarities and differences, that all these edifices, built in different manner, has the same root – bionic architecture.
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Čábelková, Nahorniaková Marcela. "Organická soudobá architektura a bydlení". Doctoral thesis, Vysoké učení technické v Brně. Fakulta architektury, 2012. http://www.nusl.cz/ntk/nusl-233242.

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Organic architecture is often used term especially in connection with the daring and experimental form. The exact definition or division is still missing. The founder of modern organic architecture is to be generally considered as Frank Loyd Wright. He was the man who created organic architecture and also made first definition in the early twentieth century, when formulating new architectural styles. Dissertation deals with insufficiently described problem of organic architecture. The main focus is on contemporary production of residential organic architecture in Central Europe. Especially on the particular realizations in Hungary, Czech Republic, Slovak Republic, Austria, Germany and Poland. Beside detail description of European realizations, the dissertation deals also with foundations of organic architecture in U.S.A. and important realizations here. Modern organic architecture primarily originated in the United States of America. The work presents most important contemporary realizations and architects creating resident living organic architecture in other countries around the world in order to complete the overview. The objective of dissertation was describing newest trends in designing buildings for living. Find position of organic architecture and give reasons for it´s increasing popularity, spread and necessity for new development in architecture according to the new trends. Organic architecture is an alternative way of contemporary residential living. It´s popularity is growing in last years in response to the development of modern technologies and materials, the need to protect our natural resources and also to the increasing negatives of globalization and to denial of local traditions and regional specificities. Aspects of organic forms were divided into three main groups according to their main characteristics: form, harmony and sustainable development. Another objective was find location of contemporary organic residential architecture in Czech Republic and abroad. It is rather spread all over the world than concentrated on certain places. Therefore I selected countries that are richer than the others on the occurrence of organic buildings. The objective was also to address architects, specialists, public and especially university teachers of architecture and students themselves. Communication with them was mainly connected with grant project in 2010. The theme of grant project was contemporary organic architecture in residential living. There were lectures about contemporary organic architecture and exhibition on the theme: Contemporary living organic architecture. The publication of the catalog was published under the title: Contemporary organic houses - Europe. I had the opportunity to visit a number of organic family houses abroad. Specifically houses in Slovakia, Hungary and Germany. Results of survey, which was realized by questions to owners and authors of chosen buildings, are included in dissertation. The mail results of dissertation are - make a detail review of organic architecture in residential living - define trends in contemporary organic architecture and residential living - obtain and process answers from owners and authors of chosen buildings within survey - analysis of organic house ( houses) in city planning and landscape - publication of catalogue focused on organic contemporary European houses - article about contemporary organic architecture and residential living will be published in 2012 on archiweb page
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Pereira, Rúben Filipe Brás. "Bioprinting of cell-responsive bioinks for skin tissue engineering". Doctoral thesis, 2018. https://hdl.handle.net/10216/118037.

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Pereira, Rúben Filipe Brás. "Bioprinting of cell-responsive bioinks for skin tissue engineering". Tese, 2018. https://hdl.handle.net/10216/118037.

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Cautela, Maria Margarida de Almeida. "Dispositivos biossensoriais baseados na associação de biomoléculas a superfícies de ouro". Master's thesis, 2017. http://hdl.handle.net/1822/45514.

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Dissertação de mestrado em Biofísica e Bionanossistemas
As superfícies de ouro têm propriedades optoelectrónicas e físico-químicas únicas, o que as torna excelentes superfícies para o desenvolvimento de plataformas de bioreconhecimento (biossensores). O ouro pode ser funcionalizado com diferentes tipos de moléculas que, por sua vez, promovem o reconhecimento de bioentidades de interesse, resultando na alteração das propriedades físico-químicas da superfície e na geração de sinais detetáveis. Este trabalho teve como objetivo a exploração de interações da superfície de ouro com moléculas funcionais que, mais tarde, promovem a imobilização de proteínas, p.ex. avidinas, como passo crucial no desenvolvimento de dispositivos biossensoriais baseados nas interações biotina-avidina. Os sistemas biotina-avidina foram escolhidos devido à sua afinidade, alta especificidade e, por fim, estabilidade. Para este fim, foram fabricados diferentes materiais de ouro para estudar interações biomoleculares: i) filmes de ouro depositados em lâminas de vidro de forma a tornar possível a organização de proteínas em monocamadas auto-montadas (self-assembled monolayers – SAMs), e ii) as nanopartículas em forma de esferas e nanovaras (nanorods) que foram utilizados como estruturas avançadas para diagnóstico. Estas superfícies foram funcionalizadas com compostos contendo tiol numa extremidade e o éster hidroxisuccinimida ou a própria biotina na outra extremidade. Alternativamente, as moléculas de funcionalização continham uma cadeia de ligação de polietilenoglicol (PEG) inerte adequado para aplicações dentro do corpo humano, uma vez que diminui a ligação não específica de bioentidades. Tanto a avidina comercial como as suas análogas recombinantes (Switchavidin e ChiAVD) foram imobilizadas com sucesso nas superfícies de ouro pré-funcionalizadas. De forma resumida, esta dissertação representa a base de estudos futuros para a investigação da deteção de biomoléculas de interesse via alterações nas superfícies. Estas alterações podem ser detetadas através de métodos óticos e/ou elétricos, tais como espetroscopia de absorção/reflexão e/ou ressonância de plasmão de superfície.
Gold surfaces have unique optoelectronic and physico-chemical properties that make them excellent surfaces for the development of biorecognition platforms (biosensors). Gold can be functionalized with different kind of molecules that in turn promote recognition of bioentities of interest, resulting in alteration of the surface physico-chemical properties and in the generation of detectable signals. This work aimed to explore the gold surface interactions with functional molecules that further allow immobilization of proteins, e.g. avidins, as crucial steps in the development of biosensor devices based on biotin-avidin interactions. The biotin-avidin systems were chosen because of their affinity, high specificity and ultimately stability. To this end, different gold materials were fabricated to study the biomolecule interactions: i) gold films were prepared on glass slides to elucidate the organization of proteins in self-assembled monolayers, and ii) nanoparticles of spherical shape and in the form of nanorods were used as advanced nanostructures for diagnostic purposes. These surfaces were functionalized with thiol-bearing hydroxysuccinimide ester or biotin itself. Alternatively, the functionalization molecules contained an inert polyethylene glycol (PEG) linker chain, suitable for in- body applications, as it decreases non-specific attachment of bioentities. Both commercial avidin and charge neutralized recombinant mutants (reversible switchavidin and thermostable chimeric avidin) were successfully immobilized on the pre-functionalized gold. This thesis represents the basis for future studies that will investigate the detection of biomolecules of interest via alterations on gold surfaces. The interactions are detectable via optical and/or electrical methods, such as absorption/reflection and/or surface plasmon resonance.
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Kim, Eun Jin. "New Strategies in the Localization of Natural Product Biosynthetic Pathways and Achieving Heterologous Expression". 2009. http://hdl.handle.net/1969.1/ETD-TAMU-2009-12-7427.

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Natural products have long furnished medical science playing a significant role in drug discovery and development. Their importance notwithstanding, it is estimated that less than 1% of microorganisms can be cultivated from environmental sources using standard laboratory techniques. It is therefore necessary to develop biochemical and genetic techniques to access these uncultivable genomes. Here as a point of departure toward this goal, two cDNA libraries of two microorganisms were constructed along with five fosmid libraries with DNA isolated from marine environmental samples. We describe the construction of cDNA libraries from marine microbial species and detail experiments to exploit these libraries for their natural product biosynthetic pathways and other metabolic enzymes they harbor. However, no useful biosynthetic pathways were detected within the cDNA libraries. Genetic selection by complementation was additionally explored as a method to identify and localize biosynthetic gene clusters within marine microbial DNA libraries. Genetic selection is a fast and economic method which utilizes selection of a part of a pathway to represent the presence of an entire pathway for the complementation of known mutant strains. We describe genetic selection to localize biotin biosynthetic pathways of Hon6 (Chromohalobacter sp.) as a proof of principle experiment for the identification and localization of biosynthetic pathways in general. Instead of developing purification methods or manipulating cultivation conditions, large fragments of non-culturable bacterial genomes can be cloned and expressed using recombinant DNA technology. A strong transcriptional promoter to control high-level gene expression is required in recombinant expression plasmids. We aimed to develop new tools to control gene expression through the use of riboswitches. Riboswitches are metabolite-sensing ribonucleic acid (RNA) elements that possess the remarkable ability to control gene expression. The thiamine pyrophosphate (TPP) riboswitch system was chosen as it will enable use of E. coli as a suitable host strain. This system is particularly attractive because it has one of the simplest structures among the riboswitches elucidated to date. The use of the TPP riboswitch will also enable modulation of pathway gene expression by varying the TPP coccentration as many gene products are toxic. The violacein gene cluster from Chromobacterium violaceum was selected and placed under the control of this riboswitch. We describe modulation of heterologous gene expression by the ThiC/Riboswitch and detail experiments to investigate the expression and manipulation of the gene cluster under various promoters.
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Paul, Subhadeep. "Studies on BODIPY Appended Ruthenium(II) Complexes for Bioimaging and Photodynamic Therapy Applications". Thesis, 2022. https://etd.iisc.ac.in/handle/2005/5894.

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Photodynamic therapy (PDT) is a medical technique that utilizes light, oxygen, and a photosensitizer to treat several medical conditions, including cancer. Because of the limitations and side effects of traditional anticancer therapies like surgery, chemotherapy, and radiation therapy, PDT has been recognized as an adjuvant and, in some cases, a mainstream alternative. Currently, clinical PDT utilizes tetrapyrrolic photosensitizers that possess several drawbacks. Even worldwide approved gold standard photosensitizer Photofrin® requires a high dose for the therapeutic effect that results in undesirable side effects like skin sensitivity and hepatotoxicity. In recent years, transition metal complexes are gaining interest as new photosensitizers with their fine-tuned photophysical and biological properties. This thesis work presents the results from a systematic study on the design and synthesis of new boron-dipyrromethene (BODIPY) appended ruthenium(II)complexes to study their photoinduced reactive oxygen species (ROS) generation ability, light-induced cytotoxicity and cellular imaging ability. Here, a wide range of ruthenium coordination units are connected to BODIPY chromophore with various linkers, and their effect on photophysical and photobiological properties investigated. New ruthenium(II) complexes with formulations [Ru(L1/2)(L3/4)Cl]Cl where L1, L2 (having biotin) are NN-donor bidentate phenanthroline derivatives and L3, L4 (contain BODIPY) are NNN-donor tridentate dipicolylamine derivative, were synthesized, characterized and their photocytotoxicity evaluated. The complex having both BODIPY and cancer targeting biotin showed cancer cells elective PDT effect giving respective IC50 value of 0.98±0.04 and 3.9±0.4 μM in A549 (lung cancer) and HPL1D (noncancerous) cell lines in visible light of 400-700 nm, while being non-toxic in the dark. Analogous complexes containing di-styryl BODIPY were prepared and their PDT activity with redlight activation was evaluated. The active complex produced a remarkable photocytotoxicity index (PI, ratio of IC50 in dark and with light exposure) of >5000 with red light (600- 720 nm) activation. Next, a series of bichromophoric systems having a heteroleptic [Ru(tpy)2] (tpy = terpyridine) moiety covalently linked to a BODIPY pendant were prepared, characterized and their photophysical and photobiological properties were evaluated. In a following study, a series of biotin-conjugated compounds containing BODIPY or Ru(II)-bis-tpy or both chromophoric units were developed and the difference of multichromophoric Ru-BODIPY conjugates with structurally similar compounds having a single chromophore were investigated highlighting the importance of constructing such bichromophoric systems. These bichromophoric systems produced both superoxide and singlet oxygen via dual type-I/II photosensitization processes and exerted potent apoptotic PDT effect with visible light activation. Finally, two homoleptic complexes with formulation [Ru(tpy-BODIPY)2]Cl2,where the connection between the BODIPY unit and the Ru(II)-bis-tpy scaffold differs by a phenylacetylene spacer, were prepared as PDT agents with simple molecular design and the effect of the spacer was studied in terms of structure-activity relationship. In summary, this thesis work presents systematic developments of Ru(II)-BODIPY conjugates as novel photosensitizers and photodetection agents for phototherapeutic applications.
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Livros sobre o assunto "Natural bioink"

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Benyus, Janine M. Biomimicry. New York: HarperCollins, 2009.

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Colombo, Barbara. Bionic design: Lo sviluppo del prodotto industriale attraverso lo studio della natura. Roma: Aracne, 2009.

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Kreuzer, Franz. Nobelpreis für den Lieben Gott: Chancen und Grenzen der Bionik, Wunder und Rätsel der Evolution, offene und versperrte Tore der Erkenntnis. Wien: Kremayr & Scheriau/Orac, 2004.

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Arkhitekturnai︠a︡ bionika: Problemi teorii i praktiki. MOSKVA: Kartolitografii︠a︡, 1986.

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Benyus, Janine M. Biomimicry: Innovation Inspired by Nature. Harper Perennial, 2002.

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Biomimicry: Innovation Inspired by Nature. New York: Morrow, 1997.

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Biomimicry: Innovation Inspired by Nature. Harper Perennial, 2002.

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Biomimicry: Innovation Inspired by Nature. Perennial (HarperCollins), 1998.

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9

Bion Experiments: On the Origin of LIfe. Wilhelm Reich Infant Trust, 2023.

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The Bion Experiments: On the Origin of LIfe. Wilhelm Reich Infant Trust, 2023.

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Capítulos de livros sobre o assunto "Natural bioink"

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Nachtigall, Werner. "Was bedeutet Bionik?" In Vorbild Natur, 1–6. Berlin, Heidelberg: Springer Berlin Heidelberg, 1997. http://dx.doi.org/10.1007/978-3-642-60866-7_1.

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Nachtigall, Werner. "Technische Biologie und Bionik". In Technik und Natur, 99–112. Berlin, Heidelberg: Springer Berlin Heidelberg, 1994. http://dx.doi.org/10.1007/978-3-662-01104-1_4.

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Scheele, Irmtraut. "Bionik — Entstehen einer Wissenschaft". In Technik und Natur, 193–204. Berlin, Heidelberg: Springer Berlin Heidelberg, 1994. http://dx.doi.org/10.1007/978-3-662-01104-1_8.

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Hoppe, Nils. "The Regulation of Biobanking in Germany". In GDPR and Biobanking, 277–90. Cham: Springer International Publishing, 2021. http://dx.doi.org/10.1007/978-3-030-49388-2_15.

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AbstractBiobanking in Germany is currently not subject to sui generis regulation. Instead, a plethora of norms from differing areas of law form the bundle of regulation that applies to biobanking. The exact shape and extent of the bundle depends on the exact configuration of the biobank. In the context of data protection, the rather fragmented nature of the regulation is to a certain extent alleviated by the direct impact of the EU General Data Protection Regulation (GDPR). In particular, the federalized system of data protection in Germany is simplified by an overarching set of norms that apply equally across the board. Whilst this is a welcome systematization of this part of the regulation of biobanking in Germany, the exact nature of the implementation of the Regulation raises novel issues in its own right. In this paper, I will outline the fragmented nature of biobank regulation in Germany, illustrate the issues on the basis of Germany’s population biobank NaKo and then discuss some of the more significant issues raised by the GDPR in the context of biobanking.
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Mohonea, Ana. "Bionic Criteria for Sustainable Labelling of Buildings". In Architecture Inspired by Nature, 171–80. Cham: Springer Nature Switzerland, 2023. http://dx.doi.org/10.1007/978-3-031-33144-2_15.

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Dușoiu, Elena-Codina. "Categories of Natural Principles and Their Adaptation to Bionic Design". In Architecture Inspired by Nature, 69–78. Cham: Springer Nature Switzerland, 2023. http://dx.doi.org/10.1007/978-3-031-33144-2_4.

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Couceiro, Mauro Costa. "Bionic Approaches and the Cyborg Culture: Human’s Phenotypic and Cognitive Extensions". In Architecture Inspired by Nature, 103–9. Cham: Springer Nature Switzerland, 2023. http://dx.doi.org/10.1007/978-3-031-33144-2_7.

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Yang, Changsheng, Junxiong Wang, Hong Liang e Herbert Peremans. "Compressed Sensing of Complex Reflections Using Range-Azimuth Dictionary in a Bionic Sonar System". In Advances in Natural Computation, Fuzzy Systems and Knowledge Discovery, 244–51. Cham: Springer International Publishing, 2019. http://dx.doi.org/10.1007/978-3-030-32456-8_26.

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Dușoiu, Elena-Codina. "The Bionic Paradigm of Light in Architecture and Design: From Animal Vision to Architectural Conception". In Architecture Inspired by Nature, 79–90. Cham: Springer Nature Switzerland, 2023. http://dx.doi.org/10.1007/978-3-031-33144-2_5.

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Arruda, Amilton José Vieira. "Didactic/Methodological Proposal: Use of the Natural Classification as an Element of Bionic Studies". In Biomimetics, Biodesign and Bionics, 1–19. Cham: Springer Nature Switzerland, 2024. http://dx.doi.org/10.1007/978-3-031-51311-4_1.

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Trabalhos de conferências sobre o assunto "Natural bioink"

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Wei, Zhenghao, Lin Kehua e Jianlin Feng. "A Bionic Natural Language Parser Equivalent to a Pushdown Automaton". In 2024 International Joint Conference on Neural Networks (IJCNN), 1–8. IEEE, 2024. http://dx.doi.org/10.1109/ijcnn60899.2024.10651539.

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Kuhnert, Ewa. "NATURE A CONSTANT INSPIRATION FOR HUMAN BEINGS � AESTHETIC CHANGES IN ARCHITECTURE OVER THE CENTURIES". In SGEM International Multidisciplinary Scientific GeoConference 24, 403–8. STEF92 Technology, 2024. https://doi.org/10.5593/sgem2024/6.1/s27.58.

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Since the prehistoric times, humans have been exploiting the immediate environment for various purposes, from the adaptation of caves for shelter to the subsequent transformation of the landscape for their own needs and this continues to the present day. The bio-inspiration movement in architecture, including bionic, biomorphic, and biomimetic architecture, is linked to the dynamic technological development initiated by the Industrial Revolution and later by the Digital Revolution. Each of these has influenced the epoch-specific representation and perception of architectural forms. The aim of this paper is to provide a synthetic picture of the relationship between architecture and nature historically. This will provide a basis for further research in the field of biological architecture. The hypothesis of the article is that we can identify two principal design approaches in contemporary architecture: a technological approach and an organic approach, which on occasion intertwine, particularly in terms of process and technical solutions. The study uses an analysis of the literature on the history of architecture in the context of human-nature relations and contemporary architectural trends. The historical overview presented in the article will assist in conceptualising potential avenues of aesthetic evolution, while references to contemporary examples in the field of bio-inspiration will facilitate the characterisation of the trend in the shaping of architectural forms.
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Hutchinson, William, e Matthew Warren. "The Nature of Data: Illusions of Reality". In 2001 Informing Science Conference. Informing Science Institute, 2001. http://dx.doi.org/10.28945/2405.

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The nature of data has changed as human technology has evolved. ‘Natural ' analogue data stimulate our senses, whilst machine produced data provides an intermediary for natural or artificial data to our senses. In the age of the cyborg (a machine with human attributes) and the bionic person (a human with machine attributes), it is possible for data totally alien from ‘natural reality' to be fed directly to the brain so by-passing the senses. This is a new form of reality, which lends itself to manipulation of a kind never experienced before. The dreams of deceivers may yet be realised on a mass scale.
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Lv, Jin, Xiao-jun Yang e Chen Guo. "Comprehensive Bionic Neuron Unified Model". In 2009 International Conference on Computational Intelligence and Natural Computing (CINC). IEEE, 2009. http://dx.doi.org/10.1109/cinc.2009.8.

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Zhang, Xiaojun, Minglu Zhang, Jianguang Sun e Xiaohui Li. "Design of a Modular Bionic Olfaction System for Robot". In 2008 Fourth International Conference on Natural Computation. IEEE, 2008. http://dx.doi.org/10.1109/icnc.2008.229.

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Wang, Lei, Yuwen Zhou e Weiwei Zhao. "Comparative Study on Bionic Optimization Algorithms for Sewer Optimal Design". In 2009 Fifth International Conference on Natural Computation. IEEE, 2009. http://dx.doi.org/10.1109/icnc.2009.89.

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Bensmaia, Sliman. "Biological and bionic hands: Natural neural coding and artificial perception". In 2015 IEEE World Haptics Conference (WHC). IEEE, 2015. http://dx.doi.org/10.1109/whc.2015.7177674.

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Gang, Cheng, Ge Shi-rong e Jiang Shi-lei. "Error Analysis and Kinematic Calibration of 3-RPS Symmetrical Parallel Bionic Leg". In 2009 Fifth International Conference on Natural Computation. IEEE, 2009. http://dx.doi.org/10.1109/icnc.2009.590.

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Chiu, Wan-ting, e Shu-Chuan Tseng. "The Influence of Bionic Creatures and Natural Condition on Design Inspirtation". In 2015 IIAI 4th International Congress on Advanced Applied Informatics (IIAI-AAI). IEEE, 2015. http://dx.doi.org/10.1109/iiai-aai.2015.218.

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Chansoria, Parth, e Rohan Shirwaiker. "Ultrasonically-Induced Patterning of Viable Cells in Viscous Bioinks During 3D Biofabrication". In ASME 2019 14th International Manufacturing Science and Engineering Conference. American Society of Mechanical Engineers, 2019. http://dx.doi.org/10.1115/msec2019-2816.

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Abstract In attempts to engineer human tissues in the lab, bio-mimicking the cellular arrangement of natural tissues is critical to achieve the required biological and mechanical form and function. Although biofabrication employing cellular bioinks continues to evolve as a promising solution over polymer scaffold based techniques in creating complex multi-cellular tissues, the ability of most current biofabrication processes to mimic the requisite cellular arrangement is limited. In this study, we propose a novel biofabrication approach that uses forces generated by bulk standing acoustic waves (BSAW) to non-deleteriously align cells within viscous bioinks. We computationally determine the acoustic pressure pattern generated by BSAW and experimentally map the effects of BSAW frequency (0.71, 1, 1.5, 2 MHz) on the linear arrangement of two types of human cells (adipose-derived stem cells and MG63) in alginate. Computational results indicate a non-linear relationship between frequency and acoustic pressure amplitude. Experimental results demonstrate that the spacing between adjacent strands of aligned cells is affected by frequency (p < 0.0001), and this effect is independent of the cell type. Lastly, we demonstrate a synergistic technique of gradual crosslinking in tandem with the BSAW-induced alignment to entrap cells within crosslinked hydrogels. This study represents an advancement in engineered tissue biofabrication aimed at bio-mimicry.
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Relatórios de organizações sobre o assunto "Natural bioink"

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Spiegel, Yitzhak, Michael McClure, Itzhak Kahane e B. M. Zuckerman. Characterization of the Phytophagous Nematode Surface Coat to Provide New Strategies for Biocontrol. United States Department of Agriculture, novembro de 1995. http://dx.doi.org/10.32747/1995.7613015.bard.

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Chemical composition and biological role of the surface coat (SC) of the root-knot nematodes, Meloidogyne spp. are described. SC proteins of M. incognita race 3 infective juveniles (J2) were characterized by electrophoresis and western blotting of extracts from radioiodine and biotin-labelled nematodes. J2 labelled with radioiodine and biotin released 125I and biotin-labelled molecules into water after 20 hours incubation, indicating that SC proteins may be loosely attached to the nematode. Antiserum to the principal protein reacted with the surface of live J2 and with surface proteins previously separated by electrophoresis. Human red blood cells (HRBC) adhered to J2 of several tylenchid nematodes over the entire nematode body. HRBC adhered also to nylon fibers coated with SC extracted from M. javanica J2; binding was Ca++/Mg++ dependent, and decreased when the nylon fibers were coated with bovine serum albumin, or pre-incubated with fucose and mannose. These experiments support a working hypothesis that RBC adhesion involves carbohydrate moieties of HRBC and carbohydrate-recognition domain(s) (CRD) distributed on the nematode surface. To our knowledge, this is the first report of a surface CRD i the phylum Nematoda. Gold-conjugated lectins and neoglycoproteins combined with silver enhancement have been used for the detection of carbohydrates and CRD, respectively, on the SC of M. javanica J2. Biotin reagents were used to trace surface proteins, specifically, on live J2. The labile and transitory nature of the SC was demonstrated by the dynamics of HRBC adherence to detergent-treated J2, J2 at different ages or fresh-hatched J2 held at various temperatures. SC recovery was demonstrated also by a SDS-PAGE profile. Monoclonal antibodies developed to a cuticular protein of M. incognita J2 gave a slight, but significant reduction in attachment of Pasteuria penetrans spores. Spore attachment as affected by several enzymes was inconsistent: alcian blue, which specifically blocks sulfyl groups, had no afffect on spore attachment. Treatment with cationized ferritin alone or catonized ferritin following monoclonal antibody caused significant decreases in spore attachment. Those results suggest a role in attachment by negatively charged groups.
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Kapulnik, Yoram, e Donald A. Phillips. Isoflavonoid Regulation of Root Bacteria. United States Department of Agriculture, janeiro de 1996. http://dx.doi.org/10.32747/1996.7570561.bard.

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The overall objective of this project was to develop a conceptual framework for enhancing root colonization by beneficial bacteria. To accomplish this aim we tested the hypothesis that production and excretion of the plant phytoalexin medicarpin can be used for creation of a special niche along the legume roots, where beneficial microorganism, such as rhizobium, will have a selective advantage. On the Israeli side it was shown that higher medicarpin levels are exuded following the application of Rhizobium meliloti to the rhizosphere but the specific biochemical pathway governing medicarpin production was not induced significantly enough to support a constant production and excretion of this molecule to the rhizosphere. Furthermore, pathogenic bacteria and chemical elicitors were found to induce higher levels of this phytoalexin and it became important to test its natural abundance in field grown plants. On the US side, the occurrence of flavonoids and nucleosides in agricultural soils has been evaluated and biologically significant quantities of these molecules were identified. A more virulent Agrobacterium tumefaciens strain was isolated from alfalfa (Medicago sativa L.) which forms tumors on a wide range of plant species. This isolate contains genes that increase competitive colonization abilities on roots by reducing the accumulation of alfalfa isoflavonoids in the bacterial cells. Following gene tagging efforts the US lab found that mutation in the bacterial efflux pump operons of this isolate reduced its competitive abilities. This results support our original hypothesis that detoxification activity of isoflavenoids molecules, based on bacterial gene(s), is an important selection mechanism in the rhizosphere. In addition, we focused on biotin as a regulatory element in the rhizosphere to support growth of some rhizosphere microorganisms and designed a bacterial gene construct carrying the biotin-binding protein, streptavidin. Expressing this gene in tobacco roots did not affect the biotin level but its expression in alfalfa was lethal. In conclusion, the collaborative combination of basic and applied approaches toward the understanding of rhizosphere activity yielded new knowledge related to the colonization of roots by beneficial microorganisms in the presence of biological active molecules exuded from the plant roots.
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