Artigos de revistas: "Lotus corniculatus"

1

Jones, David A., e Roy Turkington. "Lotus Corniculatus L." Journal of Ecology 74, n.º 4 (dezembro de 1986): 1185. http://dx.doi.org/10.2307/2260243.

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Min, B. R., G. T. Attwood, K. Reilly, W. Sun, J. S. Peters, T. N. Barry e W. C. McNabb. "Lotus corniculatuscondensed tannins decrease in vivo populations of proteolytic bacteria and affect nitrogen metabolism in the rumen of sheep". Canadian Journal of Microbiology 48, n.º 10 (1 de outubro de 2002): 911–21. http://dx.doi.org/10.1139/w02-087.

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Condensed tannins in forage legumes improve the nutrition of sheep by reducing ruminal degradation of plant protein and increasing crude protein flow to the intestine. However, the effects of condensed tannins in forage legumes on rumen bacterial populations in vivo are poorly understood. The aim of this study was to investigate the specific effects of condensed tannins from Lotus corniculatus on four proteolytic rumen bacteria in sheep during and after transition from a ryegrass (Lolium perenne) white clover (Trifolium repens) diet (i.e., low condensed tannins) to a Lotus corniculatus diet (i.e., higher condensed tannins). The bacterial populations were quantified using a competitive polymerase chain reaction. Lotus corniculatus was fed with or without ruminal infusions of polyethylene glycol (PEG), which binds to and inactivates condensed tannins, enabling the effect of condensed tannins on bacterial populations to be examined. When sheep fed on ryegrass white clover, populations of Clostridium proteoclasticum B316T, Butyrivibrio fibrisolvens C211a, Eubacterium sp. C12b, and Streptococcus bovis B315 were 1.5 × 108, 1.1 × 106, 4.6 × 108, and 7.1 × 106mL1, respectively. When the diet was changed to Lotus corniculatus, the average populations (after 8120 h) of C. proteoclasticum, B. fibrisolvens, Eubacterium sp., and S. bovis decreased (P < 0.001) to 2.4 × 107, 1.1 × 105, 1.1 × 108, and 2.5 × 105mL1, respectively. When PEG was infused into the rumen of sheep fed Lotus corniculatus, the populations of C. proteoclasticum, B. fibrisolvens, Eubacterium sp., and S. bovis were higher (P < 0.010.001) than in sheep fed Lotus corniculatus without the PEG infusion, with average populations (after 8120 h) of 4.9 × 107, 3.8 × 105, 1.9 ×108, and 1.0 ×106, respectively. Sheep fed the Lotus corniculatus diet had lower rumen proteinase activity, ammonia, and soluble nitrogen (P < 0.050.001) than sheep that were fed Lotus corniculatus plus PEG. The Lotus corniculatus diet reduced rumen nitrogen digestibility (P < 0.05) and ammonia pool size and increased the flow of undegraded feed nitrogen to the abomasum. The nitrogen intake, rumen non-ammonia nitrogen pool size, rumen microbial non-ammonia nitrogen pool size, and abomasal microbial non-ammonia nitrogen fluxes were similar both in sheep fed only Lotus corniculatus and in sheep fed Lotus corniculatus plus PEG, but nonmicrobial non-ammonia nitrogen flux to the abomasum was higher (P < 0.01) for the sheep fed only Lotus corniculatus. Although condensed tannins in Lotus corniculatus reduced the populations of some proteolytic bacteria, total ruminal microbial protein and microbial protein outflow to the abomasum were unchanged, suggesting a species-specific effect of condensed tannins on bacteria in the rumen. Key words: condensed, tannin, rumen, bacteria, PCR.

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Foo, L. Y., R. Newman, G. Waghorn, W. C. McNabb e M. J. Ulyatt. "Proanthocyanidins from Lotus corniculatus". Phytochemistry 41, n.º 2 (fevereiro de 1996): 617–24. http://dx.doi.org/10.1016/0031-9422(95)00602-8.

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Li, Xiao-qiang, Yi-yu Yang, Li-juan Chen, Yan Zhang e Ye-gao Chen. "Compounds from Lotus corniculatus". Chemistry of Natural Compounds 55, n.º 4 (julho de 2019): 719–21. http://dx.doi.org/10.1007/s10600-019-02788-3.

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Grant, William F., e Ernest Small. "The origin of the Lotus corniculatus (Fabaceae) complex: a synthesis of diverse evidence". Canadian Journal of Botany 74, n.º 7 (1 de julho de 1996): 975–89. http://dx.doi.org/10.1139/b96-122.

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Lotus corniculatus, birdsfoot trefoil, is a Eurasian perennial legume, popular in temperate climates for pasture or hay and silage production. Its ancestry is controversial. While innumerable studies of L. corniculatus and allied species have been published, comprehensive morphological, geographical, and genetic studies are still needed. Nevertheless, there is sufficient information for a provisional analysis of the origin and relationships of this important economical species. Although sometimes defined to have diploid populations, L. corniculatus essentially appears to be tetraploid. Biochemical and genetic evidence indicates that this species is an allotetraploid. Although about a dozen diploid species have been proposed as ancestral to L. corniculatus, the evidence points strongly to four of these species. Lotus uliginosus uniquely shares a rhizomatous habit, an acyanogenic factor, and a tannin characteristic with L. corniculatus, clearly showing an especially close relationship, and reflecting the possibility that it is one of two direct parental species. Principal candidates for a second parent include Lotus alpinus, Lotus japonicus, and Lotus tenuis. A phenetic analysis presented here accords well with the hypothesis favoured in this paper that L. corniculatus arose as a hybrid of L. tenuis and L. uliginosus. Cytogenetic evidence indicates that L. japonicus is especially closely related to L. corniculatus. Flower colour in the hybrids between L. uliginosus and L. tenuis suggest maternal inheritance and that L. tenuis could have been the female parent. An evolutionary sequence is suggested in which L. uliginosus hybridized with L. tenuis followed by introgression from L. alpinus and L. japonicus. Alternatively, a prototype of L. alpinus, L. japonicus, and L. tenuis could have been the female parent of L. corniculatus, based on evidence from maternal inheritance of flower colour intensity and interactions of Rhizobium strains. Keywords: polyploidy, hybridization, evolution, Lotus species, Fabaceae, birdsfoot trefoil, Lotus corniculatus.

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ADU, E. K., T. N. BARRY, P. R. WILSON e P. D. KEMP. "Evaluation of Lotus corniculatus for increasing pre-weaning growth of red and hybrid deer". Journal of Agricultural Science 131, n.º 2 (setembro de 1998): 197–204. http://dx.doi.org/10.1017/s0021859698005668.

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Lactating red deer (Cervus elaphus) hinds and their calves were rotationally grazed on Lotus corniculatus or perennial ryegrass/white clover pasture at an allowance of 12 kg DM/hind/day during summer 1996 in Palmerston North, New Zealand. Half the hinds suckled pure red deer calves and half suckled hybrid (0·25 elk[ratio ]0·75 red deer) calves. Measurements were made of the diet selected, voluntary feed intake of the hinds and liveweight changes of the hinds and calves.Lotus corniculatus and perennial ryegrass constituted c. 90% of green material in the diet selected on the respective forages. Total nitrogen (N) content and organic matter digestibility (OMD) were higher for Lotus corniculatus than for perennial ryegrass/white clover pasture. Lotus corniculatus contained 21 g condensed tannin (CT)/kg dry matter (DM), whilst pasture contained only traces of CT (1·6 g/kg DM).Hinds grazing Lotus corniculatus tended to have higher voluntary feed intake, and calf liveweight gain (485 v. 399 g/day) and weaning weight (52·6 v. 48·1 kg) were greater than for deer grazing perennial ryegrass/white clover pasture. Hybrid calves grew faster than pure red deer calves (P<0·01), with hybrid calves grazing lotus having very high liveweight gain (c. 520 g/day). Liveweight gain of hinds grazing Lotus corniculatus also tended to be higher (91 v. 20 g/day) than for hinds grazing perennial ryegrass/white clover pasture. CT was bound more strongly during chewing by red deer than had been found in comparable studies with sheep and the nutritional significance of this is discussed. Nutritional reasons for the superior performance of deer grazing Lotus corniculatus are discussed.

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Ross, M. D., e W. T. Jones. "The origin of Lotus corniculatus". Theoretical and Applied Genetics 71, n.º 2 (dezembro de 1985): 284–88. http://dx.doi.org/10.1007/bf00252068.

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8

Змеева (Zmeeva), Ольга (Ol'ga) Николаевна (Nikolaevna), Наталья (Natal'ja) Эдуардовна (Eduardovna) Коломиец (Kolomiets), Наталья (Natal'ja) Юрьевна (Yur'evna) Абрамец (Abramets) e Руслан (Ruslan) Анатольевич (Anatol'evich) Бондарчук (Bondarchuk). "LOTUS CORNICULATUS L. IS A PERSPECTIVE SPECIES OF THE GENUS LOTUS L." chemistry of plant raw material, n.º 4 (15 de maio de 2017): 5–14. http://dx.doi.org/10.14258/jcprm.2017041779.

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These materials contain an overview of the most significant publications on the hare (Lotus corniculatus L.) – one of the species of the genus Lotus L., widespread in the territory of the Russian Federation, both in the wild and in culture. The prospect of Lotus corniculatus is confirmed by information about its extensive range of information about the use in the folk medicine of many countries, veterinary science, resistance to disease, the ability to grow and give high yields on different types of soils, high fodder value and melliferous value. Chemical composition of wild and cultivated of Lotus corniculatus was studied in detail. The horned lamb is a rich source of biologically active substances: proteins, polysaccharides, alkaloids, steroids, phytoalexins, saponins, tannins, flavonoids, isoflavones, phenolcarbonic acids, derivatives of anthracene, macro - and micronutrients. The diverse chemical composition of the lapwing explains the wide range of its pharmacological activity (antimicrobial, antifungal, anti-inflammatory, antiparasitic, wound-healing, antiproliferative), use in agriculture, folk medicine, veterinary medicine, cosmetology, and also substantiates the perspectivity of the species for further scientific and practical phytochemical and pharmacological research.

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9

Barry, T. N., P. D. Kemp, C. A. Ramirez-Restrepo e N. Lopezvillalobos. "Sheep production and agronomic performance of Lotus corniculatus under dryland farming". NZGA: Research and Practice Series 11 (1 de janeiro de 2003): 109–15. http://dx.doi.org/10.33584/rps.11.2003.3003.

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Lotus corniculatus (lotus) contains condensed tannins (CT; 25-35 g/kg DM), which reduce the microbial degradation of forage protein in the rumen and increase amino acid absorption from the small intestine. In grazing experiments at Palmerston North during the 1990s, sheep grazing L. corniculatus had superior wool production, body growth and ovulation rates (OR) relative to sheep grazing lucerne (Medicago sativa) or perennial ryegrass (Lolium perenne)/white clover (Tritolium repens) pasture. Polyethylene glycol (PEG) drenching studies showed that action of CT was responsible for a component of the increases in wool growth and OR, but not body growth, and increased milk yield in lactating ewes. The 'Massey lotus' programme moved in 2000 to Riverside farm in the Wairarapa, where L. corniculatus is more agronomically suited, and its integration into dryland farming systems is being studied. We aim to develop systems that increase animal productivity whilst also reducing chemical input, notably of anthelmintic drenches. In two experiments conducted over 12 weeks in spring, ewes and lambs grazing L. corniculatus without pre-lamb drenching had lower faecal egg counts (FEC) and lower dag scores than ewes and lambs grazing pasture. Also, liveweight gain (+44%), weaning weight (+26%) and wool production (+32%) were greater for lambs grazing lotus. Weaned lambs grazing L. corniculatus over 14 weeks in summer grew faster than those grazing pasture (298 cf. 201 g/day) when regularly drenched. Reduction of anthelmintic drenching reduced the growth rates of lambs grazing L. corniculatus, but at 228 g/day this was still much faster than those grazing pasture (187 g/day). Lambs grazing L. corniculatus with restricted anthelmintic grew slightly faster than regularly drenched lambs grazing pasture. In addition, ewes mated on L. corniculatus had greater ovulation rates, lambing % and weaning % (approximately 25%) than ewes mated on pasture. Lamb weaning weight was unaffected by the ewes grazing L. corniculatus during mating, but mortality rate during the period from birth to weaning was lower for lambs that were conceived when their dams were grazing lotus. Annual (dry matter) production under grazing averaged over two years (2000-2001), was 9.5 t/ha for L. corniculatus and 8.6 t/ha for pasture. The percentages of annual production that occurred in spring, summer, autumn and winter were 49, 40, 8 and 4%, respectively, for L. corniculatus and 55, 30, 9 and 6%, respectively, for pasture. L. corniculatus has potential as a specialist feed in dryland farming systems for use during mating to increase subsequent lambing percentage and to increase lamb growth while reducing anthelmintic use. The result is more lambs being drafted at an earlier age. Key words: agronomy, body growth, condensed tannins, dry matter yield, Lotus corniculatus, reproduction, sheep, withdrawing anthelmintic, wool growth

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Řepková, J., e J. Hofbauer. "Seed pod shattering in the genus Lotus and its overcoming". Czech Journal of Genetics and Plant Breeding 45, No. 2 (1 de julho de 2009): 39–44. http://dx.doi.org/10.17221/26/2009-cjgpb.

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Birdsfoot trefoil (Lotus corniculatus L.) is a perennial species that is known for its outstanding characters as a crucial component of meadow and pasture vegetation and a highly successful fodder crop. However, its cultivation has been limited by the inability to control pod shattering. The anatomic and physiological bases of pod shattering are known and are considered to be controlled by more than one gene. This paper reviews the known causes of pod shattering and potential ways of overcoming pod indehiscence in L. corniculatus. Genetic transformation is possible in the genus Lotus; however, the useful genes determining the seed pod indehiscent character have not been identified yet. The only way of introducing pod indehiscence characters into L. corniculatus is by interspecific hybridization within the genus Lotus; embryo rescue and protoplast cultures are promising. To determine useful genotypes for crosses, investigations of pre- and post-fertilization barriers are necessary. To that end, we present here a convenient procedure for a whole-mount clearing treatment of immature seeds that leaves the cell walls of tissues intact. This is a useful technique for the study of post-fertilization barriers in Lotus.

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Reynaud, J., M. Jay e S. Blaise. "Evolution and differentiation of populations of Lotus corniculatus s.l. (Fabaceae) from the southern French Alps (Massif du Ventoux and Montagne de Lure)". Canadian Journal of Botany 69, n.º 10 (1 de outubro de 1991): 2286–90. http://dx.doi.org/10.1139/b91-287.

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A chemical analysis of the polyphenolic patterns of diploid and tetraploid populations of Lotus corniculatus s.l. (Fabaceae) growing in the southern French Alps showed that diploids from Mont Ventoux and from Montagne de Lure belong to the same chemotype. On the other hand, tetraploids showed two different chemotypes. This paper suggests the following hypothesis about the evolution of Lotus corniculatus in this area since the last glaciation: diploids could be derived from a relic diploid population scattered by glaciation and restricted within very narrow areas, whereas the tetraploids originating from the diiploids were more competitive and radiated into larger areas cleared from ice. Key words: Lotus corniculatus, birdsfoot trefoil, Fabaceae, polyploidy, polyphenolic metabolism, evolution.

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Jeelani, Syed Mudassir, Santosh Kumari e Raghbir Chand Gupta. "Male meiosis in Lotus corniculatus L." Plant Systematics and Evolution 298, n.º 10 (19 de setembro de 2012): 1977–85. http://dx.doi.org/10.1007/s00606-012-0695-4.

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O'Donoughue, Louise S., e W. F. Grant. "New sources of indehiscence for birdsfoot trefoil (Lotus corniculatus, Fabaceae) produced by interspecific hybridization". Genome 30, n.º 4 (1 de agosto de 1988): 459–68. http://dx.doi.org/10.1139/g88-077.

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An interspecific hybridization program was undertaken involving the nonshattering Lotus species L. conimbricensis and L. ornithopodioides, and species belonging to the Lotus corniculatus group, L. alpinus, L. japonicus, and L. burttii. Out of 12 cross combinations, 8 were successful. Two of these, L. alpinus × L. conimbricensis and L. burttii × L. ornithopodioides, are new hybrids. Meiosis in pollen mother cells and pollen stainability of parental species and hybrids were compared. The data indicate that the two new hybrids would have potential, via amphidiploidy, in a breeding program to reduce shattering in L. corniculatus.Key words: Lotus corniculatus, Lotus species, dehiscence, indehiscence, embryo culture, interspecific hybridization.

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Blaim, Halina, e Edmunt Nowacki. "Cyanogenesis in Lotus and Trifolium species". Acta Agrobotanica 32, n.º 1 (2015): 19–26. http://dx.doi.org/10.5586/aa.1979.003.

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The occurrence of cyanogenic plants was determined in 48 Trifolium species, 12 Lotus species, in wild population as well as in varieties of T. repens L. and Loins corniculatus L. species. In the genus Trifolium only T. nigrescens Viv. proved to be high-cyanogenic, all the remaining species are acyanogenic or low-cyanogenic. In the T. repens species varieties and wild populations include an insignificant per cent of cyanogenic plants.The genus Lotus comprises both high-cyanogenic and acyanogenic species. In the L. corniculatus species varieties include much more high-cyanogenic plants than do wild populations. It seems that in L. corniculatus the breeding went in a wrong direction, because of lack of plant selection regarding the presence of toxic compounds.

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Baali, Nacera, Abderahmane Mezrag, Mohamed Bouheroum, Fadila Benayache, Samir Benayache e Amedah Souad. "Anti-inflammatory and Antioxidant Effects of Lotus corniculatus on Paracetamol-induced Hepatitis in Rats". Anti-Inflammatory & Anti-Allergy Agents in Medicinal Chemistry 19, n.º 2 (8 de junho de 2020): 128–39. http://dx.doi.org/10.2174/1871523018666190222120752.

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Background: Herbal medicines have been used in the treatment of liver diseases for a long time. The current study was elaborated to evaluate in vitro and in vivo antioxidant and anti-inflammatory effects of Lotus corniculatus (L. corniculatus) butanolic extract. Methods: The in vitro antioxidant and anti-inflammatory properties of L. corniculatus were investigated by employing DPPH radical scavenging, H2O2 scavenging and BSA denaturation assays. In vivo antioxidant and anti-inflammatory effects of L. corniculatus were evaluated against paracetamol (APAP)-induced hepatitis in rats. L.corniculatus at doses of 100 and 200 mg/kg was administered orally once daily for seven days. Serum transaminases (AST and ALT) and lactate dehydrogenase (LDH), total bilirubin levels, liver malondialdehyde (MDA), reduced glutathione (GSH), glutathione S- transferase (GST) and superoxide dismutase (SOD) levels and inflammatory markers, such as serum Creactive protein (CRP), circulating and liver myeloperoxidase (MPO) levels were investigated. Further histopathological analysis of the liver sections was performed to support the effectiveness of L. corniculatus. Results: L. corniculatus exhibited strong antioxidant and anti-inflammatory effects in vitro. In the in vivo study, our findings demonstrate that L. corniculatus (100 and 200 mg/kg) administration led to an amelioration of APAP effects on liver histology, liver functions parameters (AST, ALT, LDH, and total bilirubin levels) and liver oxidative stress markers (MDA, GSH, GST and SOD levels). Furthermore, serum CRP, circulating MPO and liver MPO levels were declined by both doses of L. corniculatus extract. The best benefits were observed with 200 mg/kg of L. corniculatus extract. Conclusion: Antioxidant and anti-inflammatory effects of L. corniculatus extract may be due to the presence of active components.

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O'Donoughue, Louise S., J. V. Raelson e W. F. Grant. "A morphological study of interspecific hybrids in the genus Lotus (Fabaceae)". Canadian Journal of Botany 68, n.º 4 (1 de abril de 1990): 803–12. http://dx.doi.org/10.1139/b90-107.

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A morphological study of hybrids in an interspecific hybridization program involving the nonshattering Lotus species L. conimbricensis and L. ornithopodioides and species belonging to the Lotus corniculatus group, L. alpinus, L. japonicus, and L. burttii, was undertaken. Two hybrids (L. alpinus × L. conimbricensis and L. burttii × L. ornithopodioides) are newly reported. Electrophoretic phenotypes for the enzymes phosphoglucomutase and triose-phosphate isomerase confirmed their hybridity. These hybrids were both highly sterile and might provide a source of indehiscence for L. corniculatus via amphidiploidy. Preliminary shattering evaluations of the hybrids that did set seed indicated increased indehiscence is being attained to a limited extent within the L. corniculatus group.

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Gürağaç Dereli, Fatma Tuğçe, Haroon Khan, Eduardo Sobarzo-Sánchez e Esra Küpeli Akkol. "Antidepressant Potential of Lotus corniculatus L. subsp. corniculatus: An Ethnobotany Based Approach". Molecules 25, n.º 6 (12 de março de 2020): 1299. http://dx.doi.org/10.3390/molecules25061299.

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As a Turkish traditional medicinal plant, aerial parts of Lotus corniculatus L. subsp. corniculatus (Fabaceae) are used as a painkiller, antihemoroidal, diuretic and sedative. In this study, the antidepressant potential of the plant has been attempted to clarify. Extracts with water, n-Hexane, ethyl acetate, and methanol were prepared respectively from the aerial parts. Antidepressant activity of the extracts were researched by using three different in vivo test models namely a tail suspension test, antagonism of tetrabenazine-induced hypothermia, ptosis, and suppression of locomotor activity and forced swimming test on male BALB/c mice and in vitro monoamine oxidase (MAO)-A and B inhibition assays. The results were evaluated through comparing with control and reference groups, and then active compounds of the active extract have been determined. Bioassay-guided fractionation of active fraction led to the isolation of three compounds and structures of the compounds were elucidated by spectroscopic methods. The data of this study demonstrate that the MeOH extract of the aerial parts of the plant showed remarkable in vivo antidepressant effect and the isolated compounds medicarpin-3-O-glucoside, gossypetin-3-O-glucoside and naringenin-7-O-glucoside (prunin) from the active sub-fractions could be responsible for the activity. Further mechanistic and toxicity studies are planned to develop new antidepressant-acting drugs.

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WAGHORN, G. C., e I. D. SHELTON. "Effect of condensed tannins in Lotus corniculatus on the nutritive value of pasture for sheep". Journal of Agricultural Science 128, n.º 3 (maio de 1997): 365–72. http://dx.doi.org/10.1017/s0021859697004218.

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The extent to which condensed tannins (CT) in Lotus corniculatus were able to affect protein degradation and the nutritive value of pasture were evaluated at Palmerston North, New Zealand in 1992 in an indoor feeding trial with sheep and using in vitro incubations. The feeding trial involved three groups of seven young wether sheep held indoors in metabolism crates for the 32-day experimental period with one group fed freshly cut ryegrass/clover pasture (Pasture group) whilst the other two groups received a mixture of c. 37% freshly cut Lotus corniculatus and 63% pasture (dry matter (DM) basis). One of the latter groups was given twice daily oral drenches of polyethylene glycol (PEG) to bind with and remove the effects of the CT (PEG group) whilst the remaining sheep (Tannin group) were not drenched. Intakes of sheep given the mixed diets were constrained to that of the pasture group. The CT concentration in the lotus was 26·1 g/kg DM so that the mixed diets contained c. 10g CT/kg DM. Effects of CT on digestion and sheep performance were minimal. Apparent digestibility of N was lower in the Tannin group (76·4%) than PEG (79·8%) or Pasture (79·4%) sheep (P<0·001), but there were no differences in DM digestibility (75%) or wool growth from mid-side patches. Rumen ammonia and soluble protein concentrations appeared to be similar in PEG and Tannin sheep and volatile fatty acid (VFA) concentrations were similar in all groups (100 mmol/l).For incubations in vitro, several ratios of Lotus corniculatus: pasture were used to determine the effects of CT on precipitation of soluble proteins and the net yield of ammonia and gas with and without PEG. Homogenates of Lotus corniculatus leaves and pasture showed that 50% of soluble protein was precipitated by 1·3% CT in homogenate DM and there was a substantial decrease in in vitro degradation to ammonia when lotus leaf accounted for 33% or more of DM with pasture. This work emphasises the need for caution when extrapolating from in vitro to the in vivo situation and suggests that 1% CT from Lotus corniculatus may be insufficient to affect the nutritive value of fresh forages for sheep.

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Vignolio, O. R., O. N. Fernández e N. O. Maceira. "Biomass allocation to vegetative and reproductive organs in Lotus glaber and L. corniculatus (Fabaceae)". Australian Journal of Botany 50, n.º 1 (2002): 75. http://dx.doi.org/10.1071/bt01012.

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Comparative studies on reproductive and vegetative attributes between Lotus glaber Mill. and L. corniculatus L. showed that under identical growth conditions seed and pod production were higher in L. glaber than in L. corniculatus. The objective of this work was to determine whether differences in reproductive output between L. glaber and L. corniculatus are related to differences in biomass allocation and whether they can be related to the habitats of each species. We compared, in two studies, the allocation of vegetative and reproductive biomass of both species' growth in pots outdoors. The shoot to root ratio was higher in L. glaber than in L. corniculatus. Both species allocated the same biomass to stems but L. corniculatus produced fewer reproductive stems of higher specific weight. The number of pods per umbel and seeds per pod did not differ between species, but the total number of umbels, pods and seeds per plant were higher in L. glaber than in L. corniculatus. Weight per pod was higher in L. corniculatus than in L. glaber. The reproductive biomass in L. glaber was distributed in a higher number of pods and seeds that were of lower weight than in L. corniculatus. In both species, pod length and seed number per pod showed a decreasing trend during the reproductive season and both attributes were positively correlated. Some of the differences reported in reproductive and vegetative attributes are consistent with the conditions of habitat where each species grows. The results are discussed in relation to the agronomic values of these species and the factors determining their persistence and distribution in the grassland.

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Đorđe, Gatarić, Radić Vojo, Đurić Branko, Scaron arić Milenko, Čolović Zora e Petković Boro. "Morphometric characteristics of Lotus corniculatus L. genotypes". African Journal of Biotechnology 12, n.º 35 (28 de agosto de 2013): 5423–26. http://dx.doi.org/10.5897/ajb12.2302.

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Zhao, Hongmei, Lijun Chen, Yiyu Yang, Yan Zhang e Yegao Chen. "A new galloyl glycoside from Lotus corniculatus". Natural Product Research 33, n.º 8 (16 de abril de 2018): 1158–61. http://dx.doi.org/10.1080/14786419.2018.1460839.

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Nualsri, C., e P. R. Beuselinck. "Rhizomatous Lotus corniculatus: IV. Inheritance of Rhizomes". Crop Science 38, n.º 5 (setembro de 1998): 1175–79. http://dx.doi.org/10.2135/cropsci1998.0011183x003800050010x.

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Ramirez-Restrepo, C. A., P. D. Kemp, T. N. Barry e N. Lopezvillalobos. "Seasonal dry matter production of Lotus corniculatus in a dryland commercial sheep pastoral system". NZGA: Research and Practice Series 11 (1 de janeiro de 2003): 185–88. http://dx.doi.org/10.33584/rps.11.2003.3009.

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Lotus corniculatus (Birdsfoot trefoil, 'Grassland Goldie') may have a role in dryland pastoral systems in New Zealand, but there is little information available on its production under sheep grazing in these conditions. Dry matter (DM) production of L. corniculatus was measured monthly for 2 consecutive years in a systems approach experiment comparing it with perennial ryegrass (Lolium perenne)/white clover (Trifolium repens) pasture in the Wairarapa on the east coast of the North Island. The total DM produced for 2 years was 8.5 and 10.5 t /ha for L. corniculatus compared with 7.3 and 9.9 t /ha for pasture. Also, the moderate concentration of CT (18- 29 g/kg DM) in L. corniculatus has been shown to increase sheep productivity (Wang et al. 1996ab; Min et al. 1999, 2001). This production advantage, combined with improved sheep performance, supports the potential use of L. corniculatus as a specialist forage in dryland areas. Key words: condensed tannins, perennial ryegrass, Lolium perenne, white clover, Trifolium repens, pasture.

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Papadopoulos, Y. A., K. B. McRae, D. Gehl e S. A. E. Fillmore. "Bruce birdsfoot trefoil". Canadian Journal of Plant Science 88, n.º 6 (1 de novembro de 2008): 1093–94. http://dx.doi.org/10.4141/cjps08035.

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Bruce birdsfoot trefoil (Lotus corniculatus L.) is a 71-clone synthetic variety developed at the Nappan Research Farm, Agriculture and Agri-Food Canada, Nappan, Nova Scotia. Bruce was developed through two cycles of mass selection for plant vigour (top and root growth) and winterhardiness, and one cycle of mass selection for re-growth vigour under grazing. In Atlantic Canada, Bruce produced more forage than the check cultivar Leo during 3 post-seeding years. It was also superior in spring growth with high first-cut herbage yields. Key words: Birdsfoot trefoil, Lotus corniculatus, cultivar description

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Papadopoulos, Y. A., T. M. Choo, B. R. Christie, W. G. Thomas, D. B. McKenzie, G. Bélanger, K. Sutherland, K. B. McRae e S. A. E. Fillmore. "AC Langille birdsfoot trefoil". Canadian Journal of Plant Science 77, n.º 4 (1 de outubro de 1997): 653–54. http://dx.doi.org/10.4141/p96-097.

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AC Langille is a birdsfoot trefoil (Lotus corniculatus L.) cultivar developed by the Nappan Research Farm, Agriculture and Agri-Food Canada. It was developed through two cycles of mass selection for winterhardiness and one cycle of mass selection for seedling vigor. The original material was six distinct germplasms selected from the cultivar Leo and released from the Crop Science Department of the University of Guelph. AC Langille is superior to the check cultivar Leo in forage yield and seedling vigor in Atlantic Canada. Key words: Birdsfoot trefoil, Lotus corniculatus, cultivar description, seedling vigor

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Castillo, A., M. Rebuffo, M. Dalla Rizza, G. Folle, F. Santiñaque, O. Borsani e J. Monza. "Generation and Characterization of Interspecific Hybrids of Lotus uliginosus × Lotus corniculatus". Crop Science 52, n.º 4 (julho de 2012): 1572–82. http://dx.doi.org/10.2135/cropsci2011.07.0374.

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Borowicz, Victoria A. "Effects of benomyl, clipping, and competition on growth of prereproductive Lotus corniculatus". Canadian Journal of Botany 71, n.º 9 (1 de setembro de 1993): 1169–75. http://dx.doi.org/10.1139/b93-137.

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Vesicular–arbuscular mycorrhizae, defoliation, and competition can influence survival, growth, and fecundity of plants, but the combined effects of these factors are not well known. I examined how combinations of these factors influence biomass allocation and investment in root nodules by prereproductive Lotus corniculatus and whether the effects were ephemeral. Soil with vesicular–arbuscular mycorrhizal (VAM) fungi was treated with the fungicide benomyl or water and added to trays containing two L. corniculatus or one L. corniculatus and one Brassica napus (a nonmycotrophic species). Leaves of target L. corniculatus were undamaged or clipped five times over 40 days. Plants were harvested 5, 18, or 36 days after last clipping. Interspecific competition was the dominant effect at all harvests: B. napus greatly depressed growth of its neighbor. Benomyl depressed VAM colonization only in the first harvest, and growth reduction associated with depressed colonization diminished over time. Clipping reduced growth most in plants paired with conspecifics, but growth depression was transient. Benomyl and clipping reduced mass of root nodules in the first harvest. Benomyl reduced root mass in nontarget (competitor) L. corniculatus, but plants recovered with time. Neither benomyl nor clipping of the target plant affected B. napus. Interactions were few, indicating that the effects of factors were mostly additive. Key words: VAM fungi, resource allocation, nonmycotrophic competitor.

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Kumar, Shalini, Alexandre Bourdès e Philip Poole. "De Novo Alanine Synthesis by Bacteroids of Mesorhizobium loti Is Not Required for Nitrogen Transfer in the Determinate Nodules of Lotus corniculatus". Journal of Bacteriology 187, n.º 15 (1 de agosto de 2005): 5493–95. http://dx.doi.org/10.1128/jb.187.15.5493-5495.2005.

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ABSTRACT Deletion of both alanine dehydrogenase genes (aldA) in Mesorhizobium loti resulted in the loss of AldA enzyme activity from cultured bacteria and bacteroids but had no effect on the symbiotic performance of Lotus corniculatus plants. Thus, neither indeterminate pea nodules nor determinate L. corniculatus nodules export alanine as the sole nitrogen secretion product.

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Judith Webb, K., Sue Jones, Mark P. Robbins e Frank R. Minchin. "Characterization of transgenic root cultures of Trifolium repens, Trifolium pratense and Lotus corniculatus and transgenic plants of Lotus corniculatus". Plant Science 70, n.º 2 (janeiro de 1990): 243–54. http://dx.doi.org/10.1016/0168-9452(90)90139-f.

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Pankhurst, Clive E., Douglas H. Hopcroft e William T. Jones. "Comparative morphology and flavolan content of Rhizobium loti induced effective and ineffective root nodules on Lotus species, Leuceana leucocephala, Carmichaelia flagelliformis, Ornithopus sativus, and Clianthus puniceus". Canadian Journal of Botany 65, n.º 12 (1 de dezembro de 1987): 2676–85. http://dx.doi.org/10.1139/b87-358.

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The morphology of Rhizobium loti induced root nodules and the flavolan content of nodulated roots of several Lotus species, Leuceana leucocephala, Carmichaelia flagelliformis, Ornithopus sativus, and Clianthus puniceus were examined. Rhizobium loti strain NZP2037 formed effective (Nod+Fix+) nodules on all legumes, but strain NZP2213 formed Nod+Fix+ nodules only on Lotus corniculatus var. cree and ineffective (Nod+Fix−) nodules on all other legumes. The Nod+Fix− nodules developed by NZP2213 showed morphologies ranging from the complete absence of bacteria within “tumour-like” structures to the development of nodules containing bacteria that were either not released or only incompletely released from infection threads. Within nodules formed by NZP2213 on Lotus corniculatus var. hirsutus and Carmichaelia flagelliformis the rhizobia had multiplied extensively within unwalled, plasma membrane bound, infection droplets. Flavolans rich in prodelphinidin, which is toxic towards NZP2213, were present in the roots of Lotus angustissimus, Lotus pedunculatus, Lotus subbiflorus, and Leuceana leucocephala, but only trace amounts of flavolan were found in the roots of Carmichaelia flagelliformis, Ornithopus sativus, and Clianthus puniceus.

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Kramina, Tatyana E., Ilja G. Meschersky, Alina V. Fedorova, Natalia V. Vasilieva, Nina Yu Stepanova e Ivan A. Schanzer. "Is Lotus zhegulensis Klok. (Fabaceae) a separate species endemic of the Middle Volga Region or just a variety of a widespread L. corniculatus L.?" Turczaninowia 24, n.º 4 (20 de dezembro de 2021): 73–83. http://dx.doi.org/10.14258/turczaninowia.24.4.7.

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We have studied several samples from Lotus corniculatus s. l. and L. zhegulensis populations with the use of SSR and sequenced nuclear (ITS) and plastid (atpB-rbcL, ndhC-trnV, rpl32-trnL(UAG), trnH-psbA, trnL-trnF) markers. We analysed 9 local populations from the Volga River valley ranging from Ulyanovsk in the north to Volgograd in the south, as well as two local populations from Moscow and Lipetsk Provinces. Analyses of SSR markers using the STRUCRURE program divided the sample into three genetic clusters. Populations from Moscow and Lipetsk Provinces, and three local populations from the ‘locus classicus’ location of L. zhegulensis in Samara Province appeared to be the most genetically differentiated while all the other populations were variously genetically admixed. Low pairwise Fst values indicate low genetic differentiation of Lotus populations and the intraspecific nature of the revealed diversity. The nuclear and plastid DNA sequences analyzed yielded little information. Substitutions and indels revealed were mostly autapomorphies characteristic of separate specimens or small groups of specimens but not of populations. Our study enables to firmly conclude that Lotus populations from the right bank of the Volga River in vicinity of Zhiguli upland described as L. zhegulensis are weakly genetically differentiated from other populations of Lotus corniculatus s. l. and, hence, cannot be regarded as a separate species.

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YANG, J. B., D. A. SOMERS, R. L. WRIGHT e R. L. McGRAW. "SEED POD DEHISCENCE IN BIRDSFOOT TREFOIL, Lotus conimbricensis, AND THEIR INTERSPECIFIC SOMATIC HYBRID". Canadian Journal of Plant Science 70, n.º 1 (1 de janeiro de 1990): 279–84. http://dx.doi.org/10.4141/cjps90-030.

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Application of gibberellic acid to the floral stigma of a sterile somatic hybrid between birdsfoot trefoil (Lotus corniculatus L.) and L. conimbricensis Wild. induced development of seedless pods. Pods formed from gibberellic acid-treated flowers of birdsfoot trefoil, somatic hybrid, and L. conimbricensis exhibited 93%, 3%, and no dehiscence, respectively, after incubation at 15.5% relative humidity for 72 h. Indehiscence of the somatic hybrid was associated with reduced lignification of the seed pod mesocarp compared with birdsfoot trefoil as determined by phloroglucinol-HCL staining. Indehiscence was apparently transferred from L. conimbricensis, which also exhibited a low level of mesocarp lignification.Key words: Lotus corniculatus L., protoplast fusion, pod dehiscence

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Zhou, Meiliang, Zhanmin Sun, Jinbo Li, Dan Wang, Yixiong Tang e Yanmin Wu. "Identification of JAZ1-MYC2 Complex in Lotus corniculatus". Journal of Plant Growth Regulation 35, n.º 2 (28 de outubro de 2015): 440–48. http://dx.doi.org/10.1007/s00344-015-9551-4.

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YÜCEL, Tayyibe Beyza. "Mikrodalga ile Lotus corniculatus L. var. corniculatus Bitkisinin Uçucu Yağ Analizi ve Antimikrobiyal Aktivitesi". Türk Tarım ve Doğa Bilimleri Dergisi 7, n.º 2 (24 de abril de 2020): 381–89. http://dx.doi.org/10.30910/turkjans.725808.

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Luque, A., T. N. Barry, W. C. McNabb, P. D. Kemp e M. F. McDonald. "The effect of grazing Lotus corniculatus during late summer - autumn on reproductive efficiency and wool production in ewes". Australian Journal of Agricultural Research 51, n.º 3 (2000): 385. http://dx.doi.org/10.1071/ar99078.

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A grazing experiment was conducted at Massey University (Palmerston North, New Zealand) over 80 days in the late summer–autumn of 1998 (9 February–29 April). Reproductive performance and wool growth of ewes grazing on Lotus corniculatus (birdsfoot trefoil) were compared with those of ewes grazing perennial ryegrass–white clover pasture. A rotational grazing system with 210 mixed-age dry ewes (59.80.9 kg/ewe) was used, with 80 ewes grazing pasture [1 g of condensed tannin (CT) per kg dry matter (DM)] and 130 ewes grazing L. corniculatus (24 g CT/kg DM). Half of the ewes grazing L. corniculatus were supplemented orally, twice daily, with polyethylene glycol (PEG; MW, 3500) to inactivate the CTs. The effect of forage species and PEG supplementation on voluntary feed intake (VFI), reproductive performance (as measured by ovulation rate), and wool production was measured during 4 synchronised oestrous cycles. The ewes were restricted to maintenance feeding during the first 10 days of each oestrous cycle and then increased to ad libitum for the 6 days leading up to and including ovulation. In vitro organic matter digestibility in the selected diet was higher for lotus than for pasture (0.80 v. 0.76), with L. corniculatus containing less nitrogen than pasture (36.5 v. 40.8 g/kg organic matter). Ewes grazing on L. corniculatus produced 11% more wool and had an ovulation rate up to 14% higher than ewes grazing pasture, with neither parameter being affected by PEG supplementation. Higher mean ovulation rates of ewes grazing L. corniculatus were due to increases in fecundity (multiple ovulations/ovulating ewe; P < 0.05), with no effect on ewes cycling/ewes mated. Three cycles of grazing on L. corniculatus were required to achieve the maximum response in multiple ovulation, but most of this was achieved after 2 cycles. As the VFI of ewes grazing on L. corniculatus was not greater than that of ewes grazing pasture, the greater wool production and higher ovulation rate of ewes grazing this forage was due to improved efficiency of feed utilisation. It was concluded that feeding ewes L. corniculatus increased the efficiency of both reproduction and wool production without increasing VFI.

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Korniychuk, Oleksandr, Kateryna Kovtun, Yuriy Veklenko, Alina Babych-Poberezhna e Valentyn Yashchuk. "Biochemical composition and quality of herbal mass of Trifolium repens L. and Lotus corniculatus L. by phases of plant growth and development." Feeds and Feed Production, n.º 92 (21 de dezembro de 2021): 116–28. http://dx.doi.org/10.31073/kormovyrobnytstvo202192-11.

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Purpose. To establish the qualitative indicators of the grass mass of the Danae variety of Trifolium repens L. and the Ajax variety of Lotus corniculatus L. in accordance with the growth and development phases of plants in order to produce high-protein feed. Methods. Field, laboratory, system analysis methods were used. Results. According to our data, the leafiness of leguminous plants and the ratio of leaf mass and stems decrease from the branching phase to the flowering phase, what significantly affects individual indicators of the biochemical composition of the green mass of the first and second mowings. According to the development phases of Trifolium repens L. and Lotus corniculatus L. plants in the grass mass of both the first and second mowings, a decrease in the content of crude protein, crude fat and increase in the content of crude fiber was observed, which was due to a decrease in the leaf mass of plants and an increase in the stem mass. The ratio of the mass of leaves and stems according to the phases of plant development of Trifolium repens L. and Lotus corniculatus L. also influenced the nutritional value of the grass mass forage. The content of fodder units from the branching phase to flowering decreased from 0.94-0.90 to 0.55-0.61 f.u., exchange energy – from 10.25- 10.27 to 7.31-7.86 MJ respectively. Conclusions. Significant changes in plant foliage covering and the percentage ratio of the mass of leaves and stems, their influence on indicators of the biochemical composition, quality and energy nutritional value of the grass mass of Trifolium repens L. and Lotus corniculatus L. have been proved. Changes in the composition of the grass mass were accompanied by a decrease in the content of crude protein, crude fat, crude ash and increase in crude fiber and nitrogen-free extractive substances, which led to a decrease in the quality of forage for the leafy mass of leguminous grasses. It has been established that the most high-protein and energetically nutritious forage for the grass mass of leguminous grasses (Trifolium repens L. and Lotus corniculatus L.) can be obtained by mowing the grass stand in the branching-budding phase. Subsequently, there is a significant decrease in the content of crude and digestible protein, metabolic energy and increase in the content of crude fiber and nitrogen-free extractives.

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Lorite, María J., Socorro Muñoz, José Olivares, María J. Soto e Juan Sanjuán. "Characterization of Strains unlike Mesorhizobium loti That Nodulate Lotus spp. in Saline Soils of Granada, Spain". Applied and Environmental Microbiology 76, n.º 12 (30 de abril de 2010): 4019–26. http://dx.doi.org/10.1128/aem.02555-09.

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ABSTRACT Lotus species are forage legumes with potential as pastures in low-fertility and environmentally constrained soils, owing to their high persistence and yield under those conditions. The aim of this work was the characterization of phenetic and genetic diversity of salt-tolerant bacteria able to establish efficient symbiosis with Lotus spp. A total of 180 isolates able to nodulate Lotus corniculatus and Lotus tenuis from two locations in Granada, Spain, were characterized. Molecular identification of the isolates was performed by repetitive extragenic palindromic PCR (REP-PCR) and 16S rRNA, atpD, and recA gene sequence analyses, showing the presence of bacteria related to different species of the genus Mesorhizobium: Mesorhizobium tarimense/Mesorhizobium tianshanense, Mesorhizobium chacoense/Mesorhizobium albiziae, and the recently described species, Mesorhizobium alhagi. No Mesorhizobium loti-like bacteria were found, although most isolates carried nodC and nifH symbiotic genes closely related to those of M. loti, considered the type species of bacteria nodulating Lotus, and other Lotus rhizobia. A significant portion of the isolates showed both high salt tolerance and good symbiotic performance with L. corniculatus, and many behaved like salt-dependent bacteria, showing faster growth and better symbiotic performance when media were supplemented with Na or Ca salts.

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Lee, J., PM Harris, BR Sinclair e BP Treloar. "Sulfur amino acid metabolism and protein synthesis in young sheep fed ryegrass pasture and two Lotus cultivars containing condensed tannin". Australian Journal of Agricultural Research 46, n.º 8 (1995): 1587. http://dx.doi.org/10.1071/ar9951587.

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Groups of sheep were fed either fresh ryegrass pasture, a low condensed tannin (CT) diet (Lotus corniculatus; or a medium CT diet (L. pedunculatus) to compare the effects of these feeds on methionine and cysteine metabolism, because of their significance in wool production, and on phenylalanine as an indicator of general protein metabolism. Dry matter intake (DMI) among groups, ryegrass pasture, Lotus corniculatus, L. pedunculatus, were significantly different, therefore the treatment means for metabolic measurements and wool growth were adjusted by covariate analysis using DMI. Neither diet nor DM1 had any overall effect on phenylalanine concentrations in whole blood or phenylalanine irrevirible loss rate (IRL). However there were significant (P < 0.001) diet effects on cysteine blood concentrations. Methionine effects paralleled those of cysteine, with higher concentrations in sheep fed L. pedunculatus relative to pasture fed animals. Sheep fed L. pedunculatus had much higher cysteine and methionine ILRs compared with either the pasture or L. corniculatus groups. Sheep fed the highest level of condensed tannin in their diet (l. pedunculatus) had significantly greater rates of transsulfuration, cysteine and methionine oxidation. Overall the results show that CT in L. pedunculatus gives a greater degree of 'protection' during digestion for cysteine, methionine and phenylalanine, while L. corniculatus resulted in very little advantage. Condensed tannin effects on wool production are marginal and secondary to whole body metabolic influences.

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St-Arnaud, M., e G. Vincent. "Influence of High Salt Levels on the Germination and Growth of Five Potentially Utilizable Plants for Median Turfing in Northern Climates". Journal of Environmental Horticulture 6, n.º 4 (1 de dezembro de 1988): 118–21. http://dx.doi.org/10.24266/0738-2898-6.4.118.

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We evaluated the germination and growth of Coronilla varia (crown vetch), Lotus corniculatus (birdsfoot trefoil), Medicago lupulina (black medic), Kochia scoparia (kochia) and Polygonum aviculare (prostrate knotweed) at different NaCl concentrations. No reduction of germination was observed in the five species studied for all concentrations of NaCl. High concentrations of NaCl in the soil adversely affected the growth of L. corniculatus and M. lupulina, but not of the other species. For P. aviculare, germination and growth was better with higher salt concentrations.

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Nikolic, Radomirka, Nevena Mitic, Suzana Zivkovic, D. Grubisic e Mirjana Neskovic. "Cytokinins and urea derivatives stimulate seed germination in Lotus corniculatus L." Archives of Biological Sciences 59, n.º 2 (2007): 125–28. http://dx.doi.org/10.2298/abs0702125n.

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We studied the effects of various cytokinins and urea derivatives on germination of aged seeds of in Lotus corniculatus L. The following substances were applied: N6-isoprenoid cytokinins (isopentenyl adenine and zeatin), adenine sulfate, N6-aromatic cytokinins (kinetin, benzyladenine) and their N9-ribosides, N-benzyl-9-(2- tetrahydropyranyl)adenine, and urea derivatives (diphenylurea, thidiazuron, and chloro-pyridyl phenylurea). With the exception of adenine sulfate, all cytokinins increased the percentage of seed germination up to twofold, depending on their kind and concentration. It is concluded that cytokinins may be among the missing factors in aged seeds of L. corniculatus contributing to the implementation of their full germination potential. They could be used to improve germination of both freshly harvested and aged seed samples, if necessary. .

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Stephenson, Andrew G., e James A. Winsor. "Lotus corniculatus Regulates Offspring Quality Through Selective Fruit Abortion". Evolution 40, n.º 3 (maio de 1986): 453. http://dx.doi.org/10.2307/2408568.

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Jiang, Wenbo, Lin Ma, Dengxia Yi e Yongzhen Pang. "The complete chloroplast genome sequence of Lotus corniculatus L." Mitochondrial DNA Part B 6, n.º 1 (2 de janeiro de 2021): 189–90. http://dx.doi.org/10.1080/23802359.2020.1860708.

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Scheffer-Basso, Simone Meredith, Silvia Ortiz Chini, Charise Dallazem Bertol e Pedro Alexandre Varella Escosteguy. "Variabilidade espaço-temporal de compostos fenólicos em Lotus corniculatus". Revista de Ciências Agrárias 41, n.º 2 (abril de 2018): 394–97. http://dx.doi.org/10.19084/rca18034.

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Marcos-García, Marta, Esther Menéndez, Marta Helena Ramírez-Bahena, Pedro F. Mateos, Álvaro Peix, Encarna Velazquez e Raúl Rivas. "Mesorhizobium helmanticense sp. nov., isolated from Lotus corniculatus nodules". International Journal of Systematic and Evolutionary Microbiology 67, n.º 7 (1 de julho de 2017): 2301–5. http://dx.doi.org/10.1099/ijsem.0.001942.

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Boerboom, Chris M., Donald L. Wyse e David A. Somers. "Mechanism of Glyphosate Tolerance in Birdsfoot Trefoil (Lotus corniculatus)". Weed Science 38, n.º 6 (novembro de 1990): 463–67. http://dx.doi.org/10.1017/s0043174500051304.

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The mechanism of glyphosate tolerance was investigated in nine birdsfoot trefoil selections that exhibited a threefold difference in glyphosate tolerance. Single-stemmed ramets (vegetative clones) established from the nine selections were used to evaluate tolerance to glyphosate, spray retention, and14C-glyphosate absorption and translocation in growth chamber experiments. The tolerance of greenhouse-grown ramets correlated with the tolerance of field-grown plants, indicating that tolerance was not a function of plant size or affected by environment. The nine selections differed in spray retention and14C-glyphosate translocation but not in glyphosate absorption. The differences in retention and translocation were not correlated with the level of glyphosate tolerance but could contribute to the tolerance of an individual plant. The specific activity of 5-enolpyruvylshikimate 3-phosphate synthase (EPSPS) (EC 2.5.1.19) ranged from 1.3 to 3.5 nmol min−1mg−1protein among the nine selections assayed and was positively correlated with plant tolerance level. These results indicate that the primary mechanism of glyphosate tolerance in birdsfoot trefoil is based on the level of EPSPS activity.

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Ollerton, Jeff, e Andrew Lack. "Partial predispersal seed predation in Lotus corniculatus L. (Fabaceae)". Seed Science Research 6, n.º 2 (junho de 1996): 65–69. http://dx.doi.org/10.1017/s0960258500003044.

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AbstractPredispersal seed predation may have implications for plant population dynamics and the evolution of plant traits, but assessing the level of seed predation for an individual plant is not always straightforward. Seeds of Lotus corniculatus (Fabaceae) are often only partially eaten by a weevil seed predator, Apion loti. Samples of these seeds were tested for viability and subsequent seedling vigour. A large proportion of these damaged seeds were viable, and the resulting seedlings almost as vigorous as those from undamaged seeds. The ubiquity of these findings, and their ecological significance, is discussed.

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Lorite, María J., José David Flores-Félix, Álvaro Peix, Juan Sanjuán e Encarna Velázquez. "Mesorhizobium olivaresii sp. nov. isolated from Lotus corniculatus nodules". Systematic and Applied Microbiology 39, n.º 8 (dezembro de 2016): 557–61. http://dx.doi.org/10.1016/j.syapm.2016.09.003.

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Davis, Cynthia, e Dean L. Linscott. "Tolerance of Birdsfoot Trefoil (Lotus corniculatus) to 2,4-D". Weed Science 34, n.º 3 (maio de 1986): 373–76. http://dx.doi.org/10.1017/s004317450006700x.

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Translocation and metabolism of14C-2,4-D [(2,4-dichlorophenoxy)acetic acid] and effects of 2,4-D on protein synthesis were compared in ‘T–68’ (2,4-D tolerant) and ‘Viking’ (susceptible) birdsfoot trefoil (Lotus corniculatusL.) in an attempt to elucidate some tolerance mechanisms. After14C-2,4-D was applied to upper trifoliate leaves, significantly less 2,4-D was found in stems, in leaves below the treated leaves, and in roots of T–68 compared to Viking. More 2,4-D was bound to alcohol-insoluble cellular constituents of T–68 leaves, stems, and roots. When alcohol-soluble components were fractionated, slightly more14C water-soluble compounds were found in T–68, indicating further inactivation by glycosylation. No amino acid-2,4-D conjugates were found. The rate of14CO2evolution from14C-2,4-D treated seedlings in T–68 was five times that in Viking. Protein synthesis appeared to be more rapid in T–68 but the relationship to 2,4-D was not clear. In part, 2,4-D resistance in T–68 may result from its ability to inactivate 2,4-D by differential binding and conjugation and by side chain breakdown as indicated by14CO2release.

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Koelzer, Janaína, Diana Ana Pereira, Juliana Bastos Dalmarco, Moacir Geraldo Pizzolatti e Tânia Silvia Fröde. "Evaluation of the anti-inflammatory efficacy of Lotus corniculatus". Food Chemistry 117, n.º 3 (dezembro de 2009): 444–50. http://dx.doi.org/10.1016/j.foodchem.2009.04.044.

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Raikar, S. V., R. H. Braun, C. Bryant, A. J. Conner e M. C. Christey. "Efficient isolation, culture and regeneration of Lotus corniculatus protoplasts". Plant Biotechnology Reports 2, n.º 3 (31 de maio de 2008): 171–77. http://dx.doi.org/10.1007/s11816-008-0058-3.

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