Bibliografias temáticas / Grapevine cell culture

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Literatura científica selecionada sobre o tema "Grapevine cell culture"

Autor: Grafiati

Publicado: 8 de fevereiro de 2025

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Artigos de revistas sobre o assunto "Grapevine cell culture"

Sák, Martin, Ivana Dokupilová, Daniel Mihálik, Jana Lakatošová, Marcela Gubišová e Ján Kraic. "Elicitation Phenolic Compounds in Cell Culture of Vitis vinifera L. by Phaeomoniella chlamydospora". Nova Biotechnologica et Chimica 13, n.º 2 (1 de dezembro de 2014): 162–71. http://dx.doi.org/10.1515/nbec-2015-0006.

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Abstract The in vitro cell cultures of Vitis vinifera L. cv. St. Laurent were treated with two elicitors - synthetic methyl jasmonate and natural, prepared from grapevine plant infected with the Phaeomoniella chlamydospora, the agent causing the Esca disease of grapevine. Efficiency of phenolic compounds production after elicitation of cell culture was analysed immediately after treatment (15 min, 30 min, 60 min) and later (after 24, 48, and 72 hours). The cell growth and content of phenolic compounds (+)-catechin, (-)-epicatechin, p-coumaric acid, syringaldehyde, rutin, vanillic acid, and trans-resveratrol were analysed in cultivated cells as well as in cultivation medium. Pch-treatment increased production of total polyphenols the most significantly 15 min after the elicitation and in optimal time was 2.86 times higher than in nonelicited culture and 1.44 times higher than in MeJa induced cell culture.

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Čarná, Mária, Vladimír Repka e Ernest Šturdí. "Proteomic Insight Into the Molecular Principles of Grapevine Habituation". Agriculture (Polnohospodárstvo) 57, n.º 4 (1 de dezembro de 2011): 129–36. http://dx.doi.org/10.2478/v10207-011-0013-0.

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Proteomic Insight Into the Molecular Principles of Grapevine Habituation Two-dimensional gel electrophoresis coupled to protein microarray analysis was used to examine, for the first time, the molecular mechanisms of grapevine (Vitis vinifera L., cv. Limberger) habituation. The examination of 2-D maps derived from control and habituated cell culture revealed the presence of 55 protein spots displaying a differential expression pattern. These facts have provide a molecular evidence suggesting that the habituated cells can be used as a model for study of cell differentiation and plant defense mechanisms. Cell death, extra-cellular alkalinization and expression of genes responsible for the formation of the defense-related proteins were analyzed in suspension cultures with hormonal autonomy (habituation). Results obtained using habituated grapevine cells compared with non-habituated cells were different and strongly depended on the concentration of elicitor applied.

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Carvalho, Ana, Christina Crisóstomo, Fernanda Leal e José Lima-Brito. "Morphological and Cytogenetic Responses of In Vitro-Grown Grapevine (Vitis vinifera L.) Plants from “Touriga Franca”, “Touriga Nacional” and “Viosinho” Varieties Under Water Stress". Stresses 4, n.º 4 (24 de outubro de 2024): 685–98. http://dx.doi.org/10.3390/stresses4040044.

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According to the climate projections, drought will increase in frequency and severity. Since water stress (WS) impacts a grapevine’s physiology and yield negatively, the evaluation and selection of tolerant genotypes are needed. To analyse the WS effects on the morphology and cell division of three grapevines (Vitis vinifera L.) varieties, “Touriga Franca” (TF), “Touriga Nacional” (TN) and “Viosinho” (VS), in vitro-grown plants were exposed to 10% polyethylene glycol 6000 (PEG) (−0.4 MPa) or 20% PEG (−0.8 MPa), incorporated in the culture medium, for four weeks. Control plants were kept in culture media without PEG. The VS and TN plants showed the highest mean numbers of nodes, shoots and leaves and average mitotic indexes under 20% PEG. The TF and TN plants showed the lowest frequencies of mitotic anomalies under 10% PEG. The VS plant growth was less affected by WS, but TF and TN presented more regular mitosis under moderate WS. Globally, in vitro culture constitutes a cost-effective experimental system for studying grapevine responses to WS and the preliminary selection of resilient genotypes. These approaches could be applied to study plant responses to other abiotic stresses based on additional evaluation techniques (e.g., transcriptional analyses or genome-wide association studies).

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Aleynova, Olga A., Andrey R. Suprun, Nikolay N. Nityagovsky, Alexandra S. Dubrovina e Konstantin V. Kiselev. "The Influence of the Grapevine Bacterial and Fungal Endophytes on Biomass Accumulation and Stilbene Production by the In Vitro Cultivated Cells of Vitis amurensis Rupr." Plants 10, n.º 7 (23 de junho de 2021): 1276. http://dx.doi.org/10.3390/plants10071276.

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Plant endophytes are known to alter the profile of secondary metabolites in plant hosts. In this study, we identified the main bacterial and fungal representatives of the wild grape Vitis amurensis Rupr. microbiome and investigated a cocultivation effect of the 14 endophytes and the V. amurensis cell suspension on biomass accumulation and stilbene biosynthesis. The cocultivation of the V. amurensis cell culture with the bacteria Agrobacterium sp., Bacillus sp., and Curtobacterium sp. for 2 weeks did not significantly affect the accumulation of cell culture fresh biomass. However, it was significantly inhibited by the bacteria Erwinia sp., Pantoea sp., Pseudomonas sp., and Xanthomonas sp. and fungi Alternaria sp., Biscogniauxia sp., Cladosporium sp., Didymella sp. 2, and Fusarium sp. Cocultivation of the grapevine cell suspension with the fungi Didymella sp. 1 and Trichoderma sp. resulted in cell death. The addition of endophytic bacteria increased the total stilbene content by 2.2–5.3 times, while the addition of endophytic fungi was more effective in inducing stilbene accumulation by 2.6–16.3 times. The highest content of stilbenes in the grapevine cells cocultured with endophytic fungi was 13.63 and 13.76 mg/g of the cell dry weight (DW) after cultivation with Biscogniauxia sp. and Didymella sp. 2, respectively. The highest content of stilbenes in the grapevine cells cocultured with endophytic bacteria was 4.49 mg/g DW after cultivation with Xanthomonas sp. The increase in stilbene production was due to a significant activation of phenylalanine ammonia lyase (PAL) and stilbene synthase (STS) gene expression. We also analyzed the sensitivity of the selected endophytes to eight antibiotics, fluconazole, and trans-resveratrol. The endophytic bacteria were sensitive to gentamicin and kanamycin, while all selected fungal strains were resistant to fluconazole with the exception of Cladosporium sp. All endophytes were tolerant of trans-resveratrol. This study showed that grape endophytes stimulate the production of stilbenes in grape cell suspension, which could further contribute to the generation of a new stimulator of stilbene biosynthesis in grapevine or grape cell cultures.

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Hurtado-Gaitán, Elías, Susana Sellés-Marchart, James Hartwell, Maria José Martínez-Esteso e Roque Bru-Martínez. "Down-Regulation of Phosphoenolpyruvate Carboxylase Kinase in Grapevine Cell Cultures and Leaves Is Linked to Enhanced Resveratrol Biosynthesis". Biomolecules 11, n.º 11 (5 de novembro de 2021): 1641. http://dx.doi.org/10.3390/biom11111641.

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In grapevine, trans-Resveratrol (tR) is produced as a defence mechanism against stress or infection. tR is also considered to be important for human health, which increases its interest to the scientific community. Transcriptomic analysis in grapevine cell cultures treated with the defence response elicitor methyl-β-cyclodextrin (CD) revealed that both copies of PHOSPHOENOLPYRUVATE CARBOXYLASE KINASE (PPCK) were down-regulated significantly. A role for PPCK in the defence response pathway has not been proposed previously. We therefore analysed the control of PPCK transcript levels in grapevine cell cultures and leaves elicited with CD. Moreover, phosphoenolpyruvate carboxylase (PPC), stilbene synthase (STS), and the transcription factors MYB14 and WRKY24, which are involved in the activation of STS transcription, were also analysed by RT-qPCR. The results revealed that under CD elicitation conditions PPCK down-regulation, increased stilbene production and loss of PPC activity occurs in both tissues. Moreover, STS transcripts were co-induced with MYB14 and WRKY24 in cell cultures and leaves. These genes have not previously been reported to respond to CD in grape leaves. Our findings thus support the hypothesis that PPCK is involved in diverting metabolism towards stilbene biosynthesis, both for in vitro cell culture and whole leaves. We thus provide new evidence for PEP being redirected between primary and secondary metabolism to support tR production and the stress response.

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Zhao, Liang, Shuangmei You, Hui Zou e Xin Guan. "Transcriptome Analysis and Cell Morphology of Vitis rupestris Cells to Botryosphaeria Dieback Pathogen Diplodia seriata". Genes 12, n.º 2 (27 de janeiro de 2021): 179. http://dx.doi.org/10.3390/genes12020179.

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Diplodia seriata, one of the major causal agents of Botryosphaeria dieback, spreads worldwide, causing cankers, leaf spots and fruit black rot in grapevine. Vitis rupestris is an American wild grapevine widely used for resistance and rootstock breeding and was found to be highly resistant to Botryosphaeria dieback. The defense responses of V. rupestris to D. seriata 98.1 were analyzed by RNA-seq in this study. There were 1365 differentially expressed genes (DEGs) annotated with Gene Ontology (GO) and enriched by the Kyoto Encyclopedia of Genes and Genomes (KEGG) database. The DEGs could be allocated to the flavonoid biosynthesis pathway and the plant–pathogen interaction pathway. Among them, 53 DEGs were transcription factors (TFs). The expression levels of 12 genes were further verified by real-time quantitative reverse transcription polymerase chain reaction (qRT-PCR). The aggregation of proteins on the plasma membrane, formation variations in the cytoskeleton and plasmodesmata and hormone regulations revealed a declined physiological status in V. rupestris suspension cells after incubation with the culture filtrates of D. seriata 98.1. This study provides insights into the molecular mechanisms in grapevine cells’ response to D. seriata 98.1, which will be valuable for the control of Botryosphaeria dieback.

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Sák, Martin, Ivana Dokupilová, Šarlota Kaňuková, Michaela Mrkvová, Daniel Mihálik, Pavol Hauptvogel e Ján Kraic. "Biotic and Abiotic Elicitors of Stilbenes Production in Vitis vinifera L. Cell Culture". Plants 10, n.º 3 (5 de março de 2021): 490. http://dx.doi.org/10.3390/plants10030490.

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The in vitro cell cultures derived from the grapevine (Vitis vinifera L.) have been used for the production of stilbenes treated with different biotic and abiotic elicitors. The red-grape cultivar Váh has been elicited by natural cellulose from Trichoderma viride, the cell wall homogenate from Fusarium oxysporum and synthetic jasmonates. The sodium-orthovanadate, known as an inhibitor of hypersensitive necrotic response in treated plant cells able to enhance production and release of secondary metabolite into the cultivation medium, was used as an abiotic elicitor. Growth of cells and the content of phenolic compounds trans-resveratrol, trans-piceid, δ-viniferin, and ɛ-viniferin, were analyzed in grapevine cells treated by individual elicitors. The highest accumulation of analyzed individual stilbenes, except of trans-piceid has been observed after treatment with the cell wall homogenate from F. oxysporum. Maximum production of trans-resveratrol, δ- and ɛ-viniferins was triggered by treatment with cellulase from T. viride. The accumulation of trans-piceid in cell cultures elicited by this cellulase revealed exactly the opposite effect, with almost three times higher production of trans-resveratrol than that of trans-piceid. This study suggested that both used fungal elicitors can enhance production more effectively than commonly used jasmonates.

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Brehm, Ilka, Regina Preisig-Müller e Helmut Kindl. "Grapevine Protoplasts as a Transient Expression System for Comparison of Stilbene Synthase Genes Containing cGMP-Responsive Promoter Elements". Zeitschrift für Naturforschung C 54, n.º 3-4 (1 de abril de 1999): 220–29. http://dx.doi.org/10.1515/znc-1999-3-412.

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Abstract A method for preparing elicitor-responsive protoplasts from grapevine cells kept in suspension culture was established. The protoplasts were employed in order to perform transient gene expression experiments produced by externally added plasmids. Using the gene coding for bacterial β-glucuronidase as the reporter gene, the transient expression under the control of various promoters of stilbene synthase genes were analyzed. The elicitor-responsiveness of promoters from grapevine genes and heterologous promoters were assayed: the grapevine stilbene synthase gene VST-1 and pine stilbene synthase genes PST-1, PST-2 and PST-3. Compared to the expression effected by the cauliflower mosaic virus 35S RNA-promoter, the stilbene synthase promoters caused a 2-5-fold increase in GUS-activity. Incubation of transformed protoplasts with fungal cell wall further stimulated the stilbene synthase promoters but not the 35S RNA-promoter. An even more pronounced differentiation between the promoters was observed when cGMP was included in the transient expression assays. Instead of treating transformed protoplasts with fungal cell wall we administered simultaneously cGMP and the plasmid to be tested. The cGMP-responsive increase was (a) specific concerning the nucleotide applied, (b) characteristic of grapevine protoplasts, and (c) not seen with shortened promoter-GUS constructs or GUS under the control of the 35S RNA-promoter. The highest cGMP-dependent reponse to stress was shown by the promoter of the grapevine stilbene synthase gene VST-1.

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Fujita, Keiko, Yoshinao Aoki e Shunji Suzuki. "Antidiabetic effects of novel cell culture established from grapevine, Vitis vinifera cv. Koshu". Cytotechnology 70, n.º 3 (15 de março de 2018): 993–99. http://dx.doi.org/10.1007/s10616-018-0203-y.

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Unknown, Unknown. "Elicitor applications to cell suspension culture for production of phenolic compounds in grapevine". Tarım Bilimleri Dergisi 22, n.º 1 (2016): 42–53. http://dx.doi.org/10.1501/tarimbil_0000001366.

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Mais fontes

Teses / dissertações sobre o assunto "Grapevine cell culture"

Marant, Blandine. "Étude de la biosynthèse et de la bioproduction des stilbènes de vigne et de leur oligomérisation par des approches biotechnologiques". Electronic Thesis or Diss., Reims, 2024. http://www.theses.fr/2024REIMS026.

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Les stilbènes sont des métabolites spécialisés de la vigne qui jouent un rôle important dans les réponses aux stress. Le resvératrol, principal précurseur des oligomères de stilbènes, est déjà reconnu pour ses propriétés biologiques. Les oligomères de resvératrol suscitent aussi un fort intérêt pour leurs activités biologiques dans des domaines variés. Cependant, leurs voies de synthèse sont peu caractérisées, notamment chez la vigne, et les méthodes actuelles d’obtention de ces stilbènes sont peu durables. L’objectif de cette thèse est d’étudier la biosynthèse des stilbènes dans des cultures cellulaires de vigne élicitées au méthyl-jasmonate (MeJA) et à la méthyl-β-cyclodextrine (MeβCD), afin de mieux comprendre les mécanismes enzymatiques impliqués dans la formation d’oligomères. Nous avons montré que l’élicitation par le MeJA induit la biosynthèse de différents stilbènes dans deux lignées cellulaires, dont plusieurs oligomères. L’expression hétérologue de la peroxydase 4 (PRX4) de vigne dans E. coli a révélé le rôle de cette enzyme dans la synthèse de dimères, trimères et tétramères de resvératrol. De plus, nous avons montré que le MeβCD potentialise les cellules après traitement au MeJA, augmentant ainsi l’expression des gènes associés à la synthèse du resvératrol et de ses oligomères. Nos travaux ont également montré que le MeβCD favorise l’accumulation du resvératrol, mais limite la production d’oligomères dépendante de la PRX4. Le traitement successif des deux lignées cellulaires par la MeβCD et le MeJA a aussi permis de produire plusieurs g/L de stilbènes en bioréacteur, soulignant l’intérêt industriel de ce procédé d’élicitation des cultures cellulaires
Stilbenes are specialized metabolites of grapevine that play a key role in stress response. Resveratrol, the primary precursor of stilbene oligomers, is widely recognized for its biological properties. Resveratrol oligomers are also of great interest for their pleiotropic biological activities in various applications. However, their biosynthetic pathways remain poorly characterized, especially in grapevine, and current methods of production are not sustainable. The aim of this thesis is to investigate the biosynthesis of stilbenes in grapevine cell cultures elicited with methyl-jasmonate (MeJA) and methyl-β-cyclodextrin (MeβCD) to better understand the enzymatic mechanisms involved in oligomers formation. We showed that elicitation with MeJA triggers the biosynthesis of various stilbenes, including oligomers, in two grapevine cell lines. The heterologous expression of grapevine peroxidase 4 (PRX4) in E. coli revealed the functional role of this enzyme in the synthesis of resveratrol dimers, trimers and tetramers. Furthermore, we showed that MeβCD primes grapevine cells after MeJA treatment, thereby enhancing the expression of genes associated with the biosynthesis of resveratrol and its oligomers. Our work also indicated that MeβCD enhances the accumulation of resveratrol but limits the PRX4-based synthesis of oligomers. Successive treatment with MeβCD and MeJA also triggers the production of several g/L of stilbenes in bioreactor, highlighting the industrial potential of this elicitation process in grapevine cell cultures

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Capítulos de livros sobre o assunto "Grapevine cell culture"

Almagro, Lorena, Sarai Belchí-Navarro, Ana Belén Sabater-Jara, Juan Carlos Vera-Urbina, Susana Sellés-Marchart, Roque Bru e María Angeles Pedreño. "Bioproduction of trans-Resveratrol from Grapevine Cell Cultures". In Natural Products, 1683–713. Berlin, Heidelberg: Springer Berlin Heidelberg, 2013. http://dx.doi.org/10.1007/978-3-642-22144-6_54.

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Ananga, Anthony, Vasil Georgiev, Joel Ochieng, Bobby Phills e Violeta Tsolov. "Production of Anthocyanins in Grape Cell Cultures: A Potential Source of Raw Material for Pharmaceutical, Food, and Cosmetic Industries". In The Mediterranean Genetic Code - Grapevine and Olive. InTech, 2013. http://dx.doi.org/10.5772/54592.

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Trabalhos de conferências sobre o assunto "Grapevine cell culture"

ALEXANDROV, Eugeniu. "The universality of grapevine". In International congress "Research-Innovation-Inovative Entreneurship", 303–7. Ion Creangă Pedagogical State University, 2024. https://doi.org/10.46727/c.13-14-10-2023.p303-307.

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Universalitatea viței-de-vie este o lucrare colectivă a cercetătorilor de la Universitatea de Științe Agricole și Medicină Veterinară a Banatului „Regele Mihai I al României” din Timișoara și Institutul de Genetică, Fiziologie și Protecție a Plantelor din Chișinău, editată cu suportul financiar al Ministerului Culturii din Republica Moldova. Prin această lucrare s-a încercat a se demonstra caracterul universal al viței-de-vie: fascinantă și miraculoasă, viță-de-vie a fost pentru om de-a lungul timpului o atracție permanentă, un mister și o binecuvântare, venind să satisfacă, prin strugurii și vinurile ce se produc din ei cele mai rafinate gusturi și să aline zbuciume sufletești.

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