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Artigos de revistas sobre o tema "Genetic transformation"

Autor: Grafiati

Publicado: 4 de junho de 2021

Última modificação: 9 de junho de 2025

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1

Donmez, Dicle, Ozhan Simsek, Tolga Izgu, Yildiz Aka Kacar, and Yesim Yalcin Mendi. "Genetic Transformation inCitrus." Scientific World Journal 2013 (2013): 1–8. http://dx.doi.org/10.1155/2013/491207.

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Citrus is one of the world’s important fruit crops. Recently, citrus molecular genetics and biotechnology work have been accelerated in the world. Genetic transformation, a biotechnological tool, allows the release of improved cultivars with desirable characteristics in a shorter period of time and therefore may be useful in citrus breeding programs.Citrustransformation has now been achieved in a number of laboratories by various methods.Agrobacterium tumefaciensis used mainly in citrus transformation studies. Particle bombardment, electroporation,A. rhizogenes, and a new method called RNA interference are used in citrus transformation studies in addition toA. tumefaciens. In this review, we illustrate how different gene transformation methods can be employed in different citrus species.

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2

De Bustos, A., R. Pérez, and N. Jouve. "Study of the homologous recombination genetic system to improve genetic transformation of wheat." Czech Journal of Genetics and Plant Breeding 41, Special Issue (July 31, 2012): 290–93. http://dx.doi.org/10.17221/6195-cjgpb.

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3

Gietz, R. Daniel, and Robin A. Woods. "Genetic Transformation of Yeast." BioTechniques 30, no. 4 (April 2001): 816–31. http://dx.doi.org/10.2144/01304rv02.

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4

Mathews, H., H. D. Wilde, R. E. Litz, and H. Y. Wetzstein. "GENETIC TRANSFORMATION OF MANGO." Acta Horticulturae, no. 341 (May 1993): 93–97. http://dx.doi.org/10.17660/actahortic.1993.341.8.

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5

Moss, Robert. "Genetic Transformation of Bacteria." American Biology Teacher 53, no. 3 (March 1, 1991): 179–80. http://dx.doi.org/10.2307/4449256.

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6

Bhatia, C. R., Patricia Viegas, Anjali Bhagwat, Helena Mathews, and N. K. Notani. "Genetic transformation of plants." Proceedings / Indian Academy of Sciences 96, no. 2 (June 1986): 79–112. http://dx.doi.org/10.1007/bf03053326.

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7

Ribas, Alessandra Ferreira, Luiz Filipe Protasio Pereira, and Luiz Gonzaga E. Vieira. "Genetic transformation of coffee." Brazilian Journal of Plant Physiology 18, no. 1 (March 2006): 83–94. http://dx.doi.org/10.1590/s1677-04202006000100007.

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In the last 15 years, considerable advances were made in coffee genetic transformation. Different research groups in the world have been able to transform coffee with genes for insect resistance, decaffeinated coffee, herbicide resistance and control of fruit maturation. Although the majority of the research is still limited to laboratory and greenhouse studies, initial field tests with transformed coffee are beginning to appear in the literature. In this review we provide an update on the state of coffee genetic transformation, presenting technical aspects related to tissue culture systems, strategies for selection and transformation with particle bombardment, as well as the use of Agrobacterium tumefaciens. We also discuss the potential applications of this technology, taking into consideration the benefits, the possible environmental risks, as well as market and consumer issues.

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8

Langeveld, S. A., S. Marinova, M. M. Gerrits, A. F. L. M. Derks, and P. M. Boonekamp. "GENETIC TRANSFORMATION OF LILY." Acta Horticulturae, no. 430 (December 1997): 290. http://dx.doi.org/10.17660/actahortic.1997.430.43.

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9

He, Liya, Jiao Feng, Sha Lu, Zhiwen Chen, Chunmei Chen, Ya He, Xiuwen Yi, and Liyan Xi. "Genetic transformation of fungi." International Journal of Developmental Biology 61, no. 6-7 (2017): 375–81. http://dx.doi.org/10.1387/ijdb.160026lh.

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10

Tsuda, Masataka, Mikio Karita, and Teruo Nakazawa. "Genetic Transformation inHelicobacter pylori." Microbiology and Immunology 37, no. 1 (January 1993): 85–89. http://dx.doi.org/10.1111/j.1348-0421.1993.tb03184.x.

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11

Ayres, Nicola M., and William D. Park. "Genetic Transformation of Rice." Critical Reviews in Plant Sciences 13, no. 3 (January 1994): 219–39. http://dx.doi.org/10.1080/07352689409701915.

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12

Ayres, N. M., and W. D. Park. "Genetic Transformation of Rice." Critical Reviews in Plant Sciences 13, no. 3 (1994): 219. http://dx.doi.org/10.1080/713608060.

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13

Girijashankar, V. "Genetic transformation of eucalyptus." Physiology and Molecular Biology of Plants 17, no. 1 (February 12, 2011): 9–23. http://dx.doi.org/10.1007/s12298-010-0048-0.

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14

Hatfull, Graham F. "Genetic transformation of mycobacteria." Trends in Microbiology 1, no. 8 (November 1993): 310–14. http://dx.doi.org/10.1016/0966-842x(93)90008-f.

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15

Sinha, Raj P. "Genetic transformation and expression." Food Research International 25, no. 3 (January 1992): 248–49. http://dx.doi.org/10.1016/0963-9969(92)90146-v.

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16

Poulsen, G. B. "Genetic transformation of Brassica." Plant Breeding 115, no. 4 (September 1996): 209–25. http://dx.doi.org/10.1111/j.1439-0523.1996.tb00907.x.

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17

Mii, M., and D. P. Chin. "GENETIC TRANSFORMATION OF ORCHIDS." Acta Horticulturae, no. 878 (October 2010): 461–66. http://dx.doi.org/10.17660/actahortic.2010.878.59.

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18

Lugosi, L., W. R. Jacobs, and B. R. Bloom. "Genetic transformation of BCG." Tubercle 70, no. 3 (September 1989): 159–70. http://dx.doi.org/10.1016/0041-3879(89)90046-9.

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19

Yoo, Jin Cheol, Jung Bo Sim, Sung Jun Kim, Si Wouk Kim, and Jung Jun Lee. "Genetic transformation ofStreptomyces caespitosus." Archives of Pharmacal Research 16, no. 4 (December 1993): 300–304. http://dx.doi.org/10.1007/bf02977520.

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20

Nirsatmanto, Arif, and Koichiro Gyokusen. "GENETIC TRANSFORMATION OF Melia azedarach L., USING Agrobacterium MEDIATED TRANSFORMATION." JOURNAL OF FORESTRY RESEARCH 4, no. 1 (March 30, 2007): 1–8. https://doi.org/10.20886/ijfr.2007.4.1.1-8.

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This study was subjected to observe the possibility of  introducing specific foreign genes into <em>Melia azedarach </em>L., using <em>Agrobacterium </em>mediated transformation. <em>Agrobacterium tumefaciens </em>used in this study consisted of  strains of  EHA105 (vector plasmid pBIsGFP) and EHA105 (vector plasmid pBsGFP) to observe the possibility of introducing genes, and strains of EHA101 (vector plasmid pIG121-Hm) and LBA4404/ferritin (vector plasmid pBG-1) to observe the shoot organogenesis after genes transformation. Explants were collected from one cm in length excised stem of <em>in-vitro </em>plantlets. The results of the study showed that genetic transformations of <em>M. azedarach </em>could be potentially developed using <em>Agrobacterium tumefaciens </em>strains : EHA105 (pBIsGFP or pBsGFP), EHA101 (pGI121-Hm) and LBA4404/ferritin (pBG-1). The expression of  GFP (<em>green fluorescence protein</em>) signal worked successfully in this transformation  with 40% of transformation  rate for pBIsGFP and 20 % for pBsGFP.  The application of <em>Agrobacterium </em>strains of EHA101 (pIG121-Hm) and LBA4404/ferritin (pBG-1) which contained specific gene of kanamycin resistance and iron accumulation for plant growth improvement showed that adventitious shoot was well induced and elongated on the rate of 30 - 60 % of explants after genes transformation.

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21

Wang, Youshuang, Zhihua Wu, Xiaoming Li, and Xiuhua Shang. "Regeneration and Genetic Transformation in Eucalyptus Species, Current Research and Future Perspectives." Plants 13, no. 20 (October 11, 2024): 2843. http://dx.doi.org/10.3390/plants13202843.

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Eucalyptus is an important plantation tree with a high economic value in China. The tree contributes significantly to China’s timber production. The stable and efficient Eucalyptus regeneration system and genetic transformation system are of great significance for exploring the regulatory function and possible genetic breeding capacity of important genes in the species. However, as a woody plant, Eucalyptus has problems, such as a long generation cycle, strong specificity of the regeneration system, and a low genetic conversion rate, which seriously limit the rapid development of Eucalyptus genetics and breeding programs. The present review summarizes the status of research on Eucalyptus regeneration and genetic transformation, with a focus on the effects of explants, media, plant growth regulators (PGRs), and concentrations in the Eucalyptus regeneration process. In addition, the effects of genotype, Agrobacterium, antibiotics, preculture, and co-culture on the genetic transformation efficiency of Eucalyptus are discussed. Furthermore, the study also summarizes the problems encountered in Eucalyptus regeneration and genetic transformation, with reference to previous studies, and it outlines future developments and prospects. The aim was to provide a reference for solving the problems of genetic instability and the low transformation efficiency of eucalyptus, and to establish an efficient and stable eucalyptus regeneration and transformation system to accelerate the process of its genetic improvement.

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22

Liang, Min, Wei Li, Landa Qi, Guocan Chen, Lei Cai, and Wen-Bing Yin. "Establishment of a Genetic Transformation System in Guanophilic Fungus Amphichorda guana." Journal of Fungi 7, no. 2 (February 14, 2021): 138. http://dx.doi.org/10.3390/jof7020138.

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Fungi from unique environments exhibit special physiological characters and plenty of bioactive natural products. However, the recalcitrant genetics or poor transformation efficiencies prevent scientists from systematically studying molecular biological mechanisms and exploiting their metabolites. In this study, we targeted a guanophilic fungus Amphichorda guana LC5815 and developed a genetic transformation system. We firstly established an efficient protoplast preparing method by conditional optimization of sporulation and protoplast regeneration. The regeneration rate of the protoplast is up to about 34.6% with 0.8 M sucrose as the osmotic pressure stabilizer. To develop the genetic transformation, we used the polyethylene glycol-mediated protoplast transformation, and the testing gene AG04914 encoding a major facilitator superfamily transporter was deleted in strain LC5815, which proves the feasibility of this genetic manipulation system. Furthermore, a uridine/uracil auxotrophic strain was created by using a positive screening protocol with 5-fluoroorotic acid as a selective reagent. Finally, the genetic transformation system was successfully established in the guanophilic fungus strain LC5815, which lays the foundation for the molecular genetics research and will facilitate the exploitation of bioactive secondary metabolites in fungi.

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23

Wang, Ping. "Genetic Transformation in Cryptococcus Species." Journal of Fungi 7, no. 1 (January 15, 2021): 56. http://dx.doi.org/10.3390/jof7010056.

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Genetic transformation plays an imperative role in our understanding of the biology in unicellular yeasts and filamentous fungi, such as Saccharomyces cerevisiae, Aspergillus nidulans, Cryphonectria parasitica, and Magnaporthe oryzae. It also helps to understand the virulence and drug resistance mechanisms of the pathogenic fungus Cryptococcus that causes cryptococcosis in health and immunocompromised individuals. Since the first attempt at DNA transformation in this fungus by Edman in 1992, various methods and techniques have been developed to introduce DNA into this organism and improve the efficiency of homology-mediated gene disruption. There have been many excellent summaries or reviews covering the subject. Here we highlight some of the significant achievements and additional refinements in the genetic transformation of Cryptococcus species.

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24

Ledig, F. Thomas. "Genetic Transformation in Forest Trees." Forestry Chronicle 61, no. 5 (October 1, 1985): 454–58. http://dx.doi.org/10.5558/tfc61454-5.

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25

Liu, Jing, Wenjing Qian, Dan Song, and Zhengquan He. "Genetic transformation of moss plant." African Journal of Biotechnology 12, no. 3 (January 16, 2013): 227–32. http://dx.doi.org/10.5897/ajbx12.008.

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26

Diaga, Diouf. "Genetic transformation of forest trees." African Journal of Biotechnology 2, no. 10 (October 31, 2003): 328–33. http://dx.doi.org/10.5897/ajb2003.000-1068.

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27

Druart, Ph, F. Delporte, M. Brazda, C. Ugarte-Ballon, A. da Câmara Machado, M. Laimer da Câmara Machado, J. Jacquemin, and B. Watillon. "GENETIC TRANSFORMATION OF CHERRY TREES." Acta Horticulturae, no. 468 (July 1998): 71–76. http://dx.doi.org/10.17660/actahortic.1998.468.5.

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28

Tepfer, D. "Genetic transformation using Agrobacterium rhizogenes." Physiologia Plantarum 79, no. 1 (May 1990): 140–46. http://dx.doi.org/10.1111/j.1399-3054.1990.tb05876.x.

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29

Tepfer, D. "Genetic transformation using Agrobacterium rhizogenes." Physiologia Plantarum 79, no. 1 (May 1990): 140–46. http://dx.doi.org/10.1034/j.1399-3054.1990.790119.x.

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30

Liu, Jinman, Justin Merritt, and Fengxia Qi. "Genetic transformation of Veillonella parvula." FEMS Microbiology Letters 322, no. 2 (July 18, 2011): 138–44. http://dx.doi.org/10.1111/j.1574-6968.2011.02344.x.

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31

Fraley, Robert T., Stephen G. Rogers, Robert B. Horsch, and Stanton B. Gelvin. "Genetic transformation in higher plants." Critical Reviews in Plant Sciences 4, no. 1 (January 1986): 1–46. http://dx.doi.org/10.1080/07352688609382217.

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32

Limami, M. Anis, Li-Yan Sun, Corinne Douat, John Helgeson, and David Tepfer. "Natural Genetic Transformation byAgrobacterium rhizogenes." Plant Physiology 118, no. 2 (October 1, 1998): 543–50. http://dx.doi.org/10.1104/pp.118.2.543.

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33

Atkinson, Peter W., Alexandra C. Pinkerton, and David A. O'Brochta. "Genetic Transformation Systems in Insects." Annual Review of Entomology 46, no. 1 (January 2001): 317–46. http://dx.doi.org/10.1146/annurev.ento.46.1.317.

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34

Baribault, T. J., K. G. M. Skene, and N. Steele Scott. "Genetic transformation of grapevine cells." Plant Cell Reports 8, no. 3 (1989): 137–40. http://dx.doi.org/10.1007/bf00716825.

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35

Cullen, D., V. Yang, T. Jeffries, J. Bolduc, and J. H. Andrews. "Genetic transformation of Aureobasidium pullulans." Journal of Biotechnology 21, no. 3 (December 1991): 283–88. http://dx.doi.org/10.1016/0168-1656(91)90048-z.

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36

Girijashankar, V., and V. Swathisree. "Genetic transformation of Sorghum bicolor." Physiology and Molecular Biology of Plants 15, no. 4 (October 2009): 287–302. http://dx.doi.org/10.1007/s12298-009-0033-7.

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37

Navani, Naveen K., Meenal A. Joshi, and Kanak L. Dikshit. "Genetic transformation of Vitreoscilla sp." Gene 177, no. 1-2 (January 1996): 265–66. http://dx.doi.org/10.1016/0378-1119(96)00284-3.

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38

Hynes, Michael J. "Genetic transformation of filamentous fungi." Journal of Genetics 75, no. 3 (December 1996): 297–311. http://dx.doi.org/10.1007/bf02966310.

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39

Seabra, R. C., and M. S. Pais. "Genetic transformation of European chestnut." Plant Cell Reports 17, no. 3 (January 1998): 177–82. http://dx.doi.org/10.1007/s002990050374.

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40

Tsvetkov, I., V. Tsolova, and A. Atanassov. "Genetic Transformation of Grape (Review)." Biotechnology & Biotechnological Equipment 11, no. 1-2 (January 1997): 23–28. http://dx.doi.org/10.1080/13102818.1997.10818911.

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41

Gaustad, P., Jorunn Eriksen, and S. D. Henriksen. "Genetic Transformation in Streptococcus Sanguis." Acta Pathologica Microbiologica Scandinavica Section B Microbiology 87B, no. 1-6 (August 15, 2009): 117–22. http://dx.doi.org/10.1111/j.1699-0463.1979.tb02413.x.

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42

Gaustad, P. "Genetic Transformation in Streptococcus Sanguis." Acta Pathologica Microbiologica Scandinavica Section B Microbiology 87B, no. 1-6 (August 15, 2009): 123–28. http://dx.doi.org/10.1111/j.1699-0463.1979.tb02414.x.

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43

GAUSTAD, P. "GENETIC TRANSFORMATION IN STREPTOCOCCUS SANGUIS." Acta Pathologica Microbiologica Scandinavica Section B Microbiology 89B, no. 1-6 (August 19, 2009): 67–73. http://dx.doi.org/10.1111/j.1699-0463.1981.tb00155_89b.x.

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44

GAUSTAD, P., and JORUNN ERIKSEN. "GENETIC TRANSFORMATION OF STREPTOCOCCUS SANGUIS." Acta Pathologica Microbiologica Scandinavica Section B Microbiology 89B, no. 1-6 (August 19, 2009): 75–80. http://dx.doi.org/10.1111/j.1699-0463.1981.tb00156_89b.x.

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45

GAUSTAD, P. "GENETIC TRANSFORMATION IN STREPTOCOCCUS SANGUIS." Acta Pathologica Microbiologica Scandinavica Series B: Microbiology 91B, no. 1-6 (August 15, 2009): 193–200. http://dx.doi.org/10.1111/j.1699-0463.1983.tb00032.x.

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46

Gaustad, P. "Genetic Transformation in Streptococcus Sanguis." Acta Pathologica Microbiologica Scandinavica Series B: Microbiology 93B, no. 1-6 (August 15, 2009): 277–82. http://dx.doi.org/10.1111/j.1699-0463.1985.tb02889.x.

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47

Gaustad, P. "Genetic Transformation in Streptococcus Sanguis." Acta Pathologica Microbiologica Scandinavica Series B: Microbiology 93B, no. 1-6 (August 15, 2009): 283–87. http://dx.doi.org/10.1111/j.1699-0463.1985.tb02890.x.

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48

Ueno, Kei-ichiro, Yutaka Fukunaga, and Ken-ichi Arisumi. "Genetic transformation ofRhododendron byAgrobacterium tumefaciens." Plant Cell Reports 16, no. 1-2 (November 1996): 38–41. http://dx.doi.org/10.1007/bf01275445.

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49

Robichon, M. P., J. P. Renou, and R. Jalouzot. "Genetic transformation ofPelargonium X hortorum." Plant Cell Reports 15, no. 1-2 (January 1995): 63–67. http://dx.doi.org/10.1007/bf01690255.

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50

Raghuwanshi, Anshu, and Robert G. Birch. "Genetic transformation of sweet sorghum." Plant Cell Reports 29, no. 9 (June 10, 2010): 997–1005. http://dx.doi.org/10.1007/s00299-010-0885-x.

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