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Artigos de revistas sobre o assunto "FTA card simple storage"
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Köster, Pamela Carolina, Begoña Bailo, Alejandro Dashti, Carolina Hernández-Castro, Rafael Calero-Bernal, Francisco Ponce-Gordo, David González-Barrio e David Carmena. "Long-Term Preservation and Storage of Faecal Samples in Whatman® Cards for PCR Detection and Genotyping of Giardia duodenalis and Cryptosporidium hominis". Animals 11, n.º 5 (12 de maio de 2021): 1369. http://dx.doi.org/10.3390/ani11051369.
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Preservation and conservation of biological specimens, including faecal samples, is a challenge in remote areas or poor-resource settings where the cold chain cannot be maintained. This study aims at evaluating the suitability of filter cards for long-term storage of faecal samples of animal and human origin positive to the diarrhoea-causing protozoan parasites, Giardia duodenalis and Cryptosporidium hominis. Three commercially available Whatman® Filter Cards were comparatively assessed: the FTA® Classic Card, the FTA® Elute Micro Card, and the 903 Protein Saver Card. Human faecal samples positive to G. duodenalis (n = 5) and C. hominis (n = 5) were used to impregnate the selected cards at given storage (1 month, 3 months, and 6 months) periods and temperature (−20 °C, 4 °C, and room temperature) conditions. Parasite DNA was detected by PCR-based methods. Sensitivity assays and quality control procedures to assess suitability for genotyping purposes were conducted. Overall, all three Whatman® cards were proven useful for the detection and molecular characterisation of G. duodenalis and C. hominis under the evaluated conditions. Whatman® cards represent a simple, safe, and cost-effective option for the transportation, preservation, and storage of faecal samples without the need of the cold chain.
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Pedrão, Priscila Grecca, Ana Carolina de Carvalho, Júlio César Possati-Resende, Fernanda de Paula Cury, Nathália C. Campanella, Cristina Mendes de Oliveira e José Humberto Tavares Guerreiro Fregnani. "DNA Recovery Using Ethanol-Based Liquid Medium from FTA Card-Stored Samples for HPV Detection". Acta Cytologica 65, n.º 3 (2021): 264–71. http://dx.doi.org/10.1159/000515913.
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<b><i>Introduction:</i></b> Alternative methods of dry storage and transportation may be a viable alternative to the use of liquid storage medium for cervical samples, especially for screening programs in places with few resources. <b><i>Objective:</i></b> The objective of this study is to verify the viability and efficacy of human papillomavirus DNA (HPV-DNA) detection in cervical cell samples collected and stored on a Flinders Technology Associates (FTA) card (Whatman Indicating FTA<sup>®</sup> Elute Micro Card) and subsequently recovered in ethanol-based liquid medium and to compare the results to those obtained using samples stored directly in ethanol-based liquid medium. <b><i>Study Design:</i></b> Thirty-four women submitted to ETZ (excision of the transformation zone of the cervix) were included in this study. Before ETZ, 2 samples of exfoliated cervical cells were collected from each woman by a doctor and stored in ethanol-based liquid medium and on an FTA card. DNA recovery from FTA samples was performed using ethanol-based liquid medium. Detection of HPV-DNA in the samples was performed using the Cobas® 4800 HPV Test Platform. <b><i>Results and Conclusions:</i></b> The HPV-DNA detection positivity rates were 70.6% for the samples collected directly in liquid medium and 64.7% for the samples stored on the FTA card, with high detection accuracy in the DNA samples recovered from the FTA card (area under the curve = 0.958; 95% confidence interval = 0.890–1.000). The concordance between the results obtained using the 2 storage media was 94.1% (Kappa = 0.866). These preliminary results suggest that collection of cervical material on an FTA card may be an alternative to storage in liquid medium since the liquid medium has some limitations. In addition, DNA recovery from the card using ethanol-based liquid medium streamlines the workflow in the laboratory and reduces the cost associated with reagents, thereby facilitating access to the HPV test in places with few resources and potentially improving cervical cancer screening.
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Shalaby, Azhar G., Neveen R. Bakry, Abeer A. E. Mohamed e Ashraf A. Khalil. "Evaluating Flinders Technology Associates card for transporting bacterial isolates and retrieval of bacterial DNA after various storage conditions". October-2020 13, n.º 10 (2020): 2243–51. http://dx.doi.org/10.14202/vetworld.2020.2243-2251.
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Background and Aim: Flinders Technology Associates (FTA) cards simplify sample storage, transport, and extraction by reducing cost and time for diagnosis. This study evaluated the FTA suitability for safe transport and storage of Gram-positive and Gram-negative bacterial cells of animal origin on its liquid culture form and from organ impression smears (tissues) under the same routine condition of microbiological laboratory along with detecting their nucleic acid over different storage conditions. Materials and Methods: Increase in bacterial count from 104 to 107 (colony-forming units/mL) of 78 isolates representing seven bacterial species was applied onto cards. FTA cards were grouped and inoculated by these bacteria and then stored at different conditions of 24-27°C, 4°C, and –20°C for 24 h, for 2 weeks, for 1 and 3 month storage, respectively. Bacteriological examination was done, after which bacterial DNA was identified using specific primers for each bacterial type and detected by polymerase chain reaction (PCR). Results: The total percentage of recovered bacteria from FTA cards was 66.7% at 24-27–C for 24 h, the detection limit was 100% in Gram-positive species, while it was 57.4% in Gram-negative ones. Regarding viable cell detection from organ impression smears, it was successful under the previous conditions. No live bacterial cells were observed by bacteriological isolation rather than only at 24-27°C for 24 h storage. All bacterial DNA were sufficiently confirmed by the PCR technique at different conditions. Conclusion: Overall, the FTA card method was observed to be a valid tool for nucleic acid purification for bacteria of animal origin in the form of culture or organ smears regardless of its Gram type and is used for a short time only 24 h for storage and transport of live bacteria specifically Gram-positive type. Moreover, the bacterial nucleic acid was intact after storage in –20°C for 3 months and was PCR amplifiable.
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Dobbs, Larry J., Merle N. Madigan, Alexis B. Carter e Lori Earls. "Use of FTA Gene Guard Filter Paper for the Storage and Transportation of Tumor Cells for Molecular Testing". Archives of Pathology & Laboratory Medicine 126, n.º 1 (1 de janeiro de 2002): 56–63. http://dx.doi.org/10.5858/2002-126-0056-uofggf.
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Abstract Context.—Efficient methods of storing tumor specimens for molecular testing are needed in the modern surgical pathology laboratory. The FTA Gene Guard system is a novel method for the collection and room temperature storage of blood samples for DNA testing. The method uses index card–sized filter papers that provide an ideal medium on which to store tumor specimens for DNA testing. Objective.—To determine whether FTA filter paper can be used in the surgical pathology laboratory to store tumor cells for DNA testing. Design.—Cell suspensions were prepared from 60 surgical specimens, and DNA was extracted either immediately or after storage on FTA paper. The DNA extracted by each method was tested by polymerase chain reaction (PCR) for the β-globin and interferon gamma genes, and the results were compared. Fifteen lymph node specimens stored on FTA paper were then tested for immunoglobulin heavy chain (IgH) gene rearrangement by PCR, and these results were compared with those obtained for immediately extracted DNA. Setting.—University medical center. Results.—The DNA extracted from cells stored on FTA paper performed as well in the PCR as the freshly extracted DNA in nearly all cases (>95%). The results of tests for IgH gene rearrangements showed 100% concordance between the 2 methods of DNA extraction. Conclusion.—Cells from surgical specimens can be stored on FTA paper for extended lengths of time, and DNA can be extracted from these cells for PCR-based testing. FTA filter paper is a reliable medium for the storage and/or transport of tumor cells for PCR-based DNA analysis.
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Marek, Martin, Miloslav Zouhar, Ondřej Douda, Marie Maňasová e Pavel Ryšánek. "Exploitation of FTA Cartridges for the Sampling, Long-Term Storage, and DNA-Based Analyses of Plant-Parasitic Nematodes". Phytopathology® 104, n.º 3 (março de 2014): 306–12. http://dx.doi.org/10.1094/phyto-03-13-0067-r.
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The use of DNA-based analyses in molecular plant nematology research has dramatically increased over recent decades. Therefore, the development and adaptation of simple, robust, and cost-effective DNA purification procedures are required to address these contemporary challenges. The solid-phase-based approach developed by Flinders Technology Associates (FTA) has been shown to be a powerful technology for the preparation of DNA from different biological materials, including blood, saliva, plant tissues, and various human and plant microbial pathogens. In this work, we demonstrate, for the first time, that this FTA-based technology is a valuable, low-cost, and time-saving approach for the sampling, long-term archiving, and molecular analysis of plant-parasitic nematodes. Despite the complex structure and anatomical organization of the multicellular bodies of nematodes, we report the successful and reliable DNA-based analysis of nematode high-copy and low-copy genes using the FTA technology. This was achieved by applying nematodes to the FTA cards either in the form of a suspension of individuals, as intact or pestle-crushed nematodes, or by the direct mechanical printing of nematode-infested plant tissues. We further demonstrate that the FTA method is also suitable for the so-called “one-nematode-assay”, in which the target DNA is typically analyzed from a single individual nematode. More surprisingly, a time-course experiment showed that nematode DNA can be detected specifically in the FTA-captured samples many years after initial sampling occurs. Collectively, our data clearly demonstrate the applicability and the robustness of this FTA-based approach for molecular research and diagnostics concerning phytonematodes; this research includes economically important species such as the stem nematode (Ditylenchus dipsaci), the sugar beet nematode (Heterodera schachtii), and the Northern root-knot nematode (Meloidogyne hapla).
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Walker, Rosie M., Louise MacGillivray, Sarah McCafferty, Nicola Wrobel, Lee Murphy, Shona M. Kerr, Stewart W. Morris et al. "Assessment of dried blood spots for DNA methylation profiling". Wellcome Open Research 4 (6 de março de 2019): 44. http://dx.doi.org/10.12688/wellcomeopenres.15136.1.
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Background: DNA methylation reflects health-related environmental exposures and genetic risk, providing insights into aetiological mechanisms and potentially predicting disease onset, progression and treatment response. An increasingly recognised need for large-scale, longitudinally-profiled samples collected world-wide has made the development of efficient and straightforward sample collection and storage procedures a pressing issue. An alternative to the low-temperature storage of EDTA tubes of venous blood samples, which are frequently the source of the DNA used in such studies, is to collect and store at room temperature blood samples using purpose built filter paper, such as Whatman FTA® cards. Our goal was to determine whether DNA stored in this manner can be used to generate DNA methylation profiles comparable to those generated using blood samples frozen in EDTA tubes. Methods: DNA methylation profiles were obtained from matched EDTA tube and Whatman FTA® card whole-blood samples from 62 Generation Scotland: Scottish Family Health Study participants using the Infinium HumanMethylation450 BeadChip. Multiple quality control procedures were implemented, the relationship between the two sample types assessed, and epigenome-wide association studies (EWASs) performed for smoking status, age and the interaction between these variables and sample storage method. Results: Dried blood spot (DBS) DNA methylation profiles were of good quality and DNA methylation profiles from matched DBS and EDTA tube samples were highly correlated (mean r = 0.991) and could distinguish between participants. EWASs replicated established associations for smoking and age, with no evidence for moderation by storage method. Conclusions: Our results support the use of Whatman FTA® cards for collecting and storing blood samples for DNA methylation profiling. This approach is likely to be particularly beneficial for large-scale studies and those carried out in areas where freezer access is limited. Furthermore, our results will inform consideration of the use of newborn heel prick DBSs for research use.
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Almeida, C. N., T. Q. Furian, K. A. Borges, G. Perdoncini, M. J. Mauel, S. L. S. Rocha, V. P. Nascimento, C. T. P. Salle e H. L. S. Moraes. "Assessment of FTA card employment for Pasteurella multocida DNA transport and detection of virulence-associated genes in strains isolated from fowl cholera in the United States". Arquivo Brasileiro de Medicina Veterinária e Zootecnia 70, n.º 6 (dezembro de 2018): 1855–61. http://dx.doi.org/10.1590/1678-4162-9821.
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ABSTRACT Fowl Cholera (FC) is a disease caused by Pasteurella multocida. The severity of this disease is partly caused by virulence factors. Genes encoding fimbriae, capsule, sialidases and proteins for iron metabolism may be related to P. multocida’s ability to infect the host. Besides to examining DNA for the presence of virulence genes, DNA is essential for the diagnostic and FTA cards are an alternative for genetic material transport. The study aims to evaluate the viability of P. multocida DNA transport using the cards and to detect 14 virulence genes in 27 strains isolated from FC cases in the United States by multiplex-PCR. No growth was observed in any of the FTA cards, which was essential to assess the security. Furthermore, DNA detection was possible in 100% of the samples, independent of the storage period (7 to 35 days) and temperature (4°C and 37°C). ptfA, exbd-tonB, hgbA, nanB, oma87, hyaD-hyaC, sodC, hgbB, sodA, nanH and pfhA genes were detected in more than 80% of the samples. FTA cards have proven to be a viable and safe tool for DNA transport of P. multocida. A majority of genes showed a high frequency, which was similar to strains isolated from FC cases.
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Gustavsson, Inger, Monica Lindell, Erik Wilander, Anders Strand e Ulf Gyllensten. "Use of FTA card for dry collection, transportation and storage of cervical cell specimen to detect high-risk HPV". Journal of Clinical Virology 46, n.º 2 (outubro de 2009): 112–16. http://dx.doi.org/10.1016/j.jcv.2009.06.021.
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Jiang, Li Ying, Yan Zhang, Fen Fen Wang, Jie Hu, Yan Xia Yan e Guang Zhao Cui. "Construction of a Simple Electro-Chemical Analyzer". Advanced Materials Research 590 (novembro de 2012): 227–30. http://dx.doi.org/10.4028/www.scientific.net/amr.590.227.
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In this work, a computerized electro-chemical analyzer based on LabVIEW is proposed. This analyzer is made up of two main parts, the hardware part and software part. The former part consists of computer, data acquisition card, and signal conditioning module, which is clearly described in this paper. The software part includes data acquisition, data processing, data storage and data displaying that are programmed in LabVIEW. The measuring methods of cyclic voltammetry and chronoamperometry have been realized with this analyzer.
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Liu, Yin, Lu Zhang, Jin Du, Peng Zhang, Xue Li Song e Zhao Wen Li. "Empirical Design for an Advanced Embedded Remote Monitoring System Based on Computer Network Technology". Advanced Materials Research 694-697 (maio de 2013): 2638–41. http://dx.doi.org/10.4028/www.scientific.net/amr.694-697.2638.
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This study presents a design of an advanced embedded remote monitoring system based on the current computer network technology.This monitoring system carries out a data acquisition process by an embedded field monitor uploads the environmental information and alarm massages to a monitoring center computer and completes the data processing and data storage. The field monitor used in the monitoring system is composed of a data acquisition card and a network transmission card. This monitoring system with low cost simple equipments has practical accuracy that is suitable for unmanned machine room, base station, intelligent community and living house.
Teses / dissertações sobre o assunto "FTA card simple storage"
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Khoory, Haifa. "The feasibility of transferring cells from archived buccal swabs to FTA card for long term and simple storage of forensic samples". University of Western Australia. Centre for Forensic Science, 2008. http://theses.library.uwa.edu.au/adt-WU2008.0088.
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[Truncated abstract] The collection of buccal cells is common practise in the epidemiological and forensic science. Unlike venipuncture collection of blood; it is a safer, non-invasive method for collection of biological material. The methods by which these cells are collected from the inner cheek of an individual and stored are the key elements in preserving DNA. Typically, forensic samples require long term storage. Samples are commonly collected on cotton swabs and stored moist at low to ultra-low temperatures (less than -20oC). Although this is the method of choice in most forensic facilities, there are drawbacks. The samples are inherently contaminated with microflora within the oral cavity and the moisture allows a plethora of microorganisms to grow. As the time frame that has elapsed from collection to storage increases, there is an exponential increase in bacterial cells. Storage of containers containing swabs coated with cells at temperatures below 20oC is also costly due to requirements for large freezers which are running and monitored over 24 hours. In the pass 10 to 15 years, researchers have focussed on alternative ways to store buccal cells. The FTA card system by Whatman is one such development. The FTA card is unique in that it provides a means for the collection of buccal cells for storage at room temperature. DNA profiling from samples stored in this way for 11 years has been successfully achieved. The filter paper matrix of the FTA card binds and subsequently lyses cells. ... (2) The second component of this thesis describes a study which subjected cells on buccal swabs to various conditions of increased temperature over periods of time to establish if DNA could be amplified. The aim was to mimic exposure to the vigours of field conditions, particularly in the extreme local environments that prevail in the United Arab Emirates. a. Initially, buccal cells stored at -20oC over 360 days were used to mimic standard archiving procedures. The cells were subsequently transferred to FTA cards, amplified and profiled by using ABI AmpFLSTR Identifiler PCR Amplification Kit (Applied Biosystems, Foster City, CA). Complete STR profiles were successfully recovered from the archived swabs. In most cases 100% of alleles were recovered, suggesting that it is feasible to transfer DNA from properly archived buccal swabs to FTA cards. b. The second phase involved the storage of fresh swabs that had been artificially aged by using incubation temperatures ranging from 40oC to 100oC. Partial profiles resulted from artificially aged samples, indicating that the prevailing conditions prior to low temperature storage of the swabs plays an important role in ensuring cellular integrity and thus, DNA quality. Results from this study suggest that it is possible for biological samples stored under correct conditions to be transferred from swabs to FTA card. In combination, the two chapters presented in this study show that it is feasible to transfer achieved forensic biology samples from swabs to the FTA card system. However, it is necessary to ensure that the samples are treated in the correct manner so as to minimise contamination from external sources and to maintain the correct environmental state to maintain intact cells and usable DNA.
Capítulos de livros sobre o assunto "FTA card simple storage"
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Hawrylak, Peter J., e Chris Hart. "Using Radio Frequency Identification Technology to Store Patients' Medical Information". In Healthcare Ethics and Training, 641–61. IGI Global, 2017. http://dx.doi.org/10.4018/978-1-5225-2237-9.ch029.
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Radio Frequency Identification (RFID) technology enables wireless communication between a RFID reader and a RFID tag. One type, passive RFID tags, need no battery, being powered from the RFID reader's radio frequency signal. Passive RFID tags can support memories that can be used to store portions of the patient's medical history. One form factor for passive RFID tags is an employee ID (used for wireless access) or credit card form factor. This form factor allows the patient to carry their medical information with them. RFID benefits include providing information to Emergency Medical Technicians (EMTs), maintaining a patient's vaccination history and providing emergency contact information, all in a clear and unmistakable format. This simplifies information exchange during patient transfers, a cause of many preventable medical mistakes and errors. Cheap and simple systems, such as the one presented in this chapter, can reduce stress and prevent possible errors. Such systems with an intuitive human-machine interface can reduce the duration of a patient visit throughput. This system can prevent simple errors such as the administration of the wrong drug, dose, or drug omission, which is a major issue in hospitals. This requires that information be stored in a standardised manner, with limited healthcare provider access and use to protect patient privacy. This chapter explores the use of passive RFID tags to store medical information about a patient, with specific focus on storage of a child's vaccination history and safety.
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Hawrylak, Peter J., e Chris Hart. "Using Radio Frequency Identification Technology to Store Patients' Medical Information". In Advances in Healthcare Information Systems and Administration, 159–78. IGI Global, 2014. http://dx.doi.org/10.4018/978-1-4666-4546-2.ch009.
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Radio Frequency Identification (RFID) technology enables wireless communication between a RFID reader and a RFID tag. One type, passive RFID tags, need no battery, being powered from the RFID reader's radio frequency signal. Passive RFID tags can support memories that can be used to store portions of the patient's medical history. One form factor for passive RFID tags is an employee ID (used for wireless access) or credit card form factor. This form factor allows the patient to carry their medical information with them. RFID benefits include providing information to Emergency Medical Technicians (EMTs), maintaining a patient's vaccination history and providing emergency contact information, all in a clear and unmistakable format. This simplifies information exchange during patient transfers, a cause of many preventable medical mistakes and errors. Cheap and simple systems, such as the one presented in this chapter, can reduce stress and prevent possible errors. Such systems with an intuitive human-machine interface can reduce the duration of a patient visit throughput. This system can prevent simple errors such as the administration of the wrong drug, dose, or drug omission, which is a major issue in hospitals. This requires that information be stored in a standardised manner, with limited healthcare provider access and use to protect patient privacy. This chapter explores the use of passive RFID tags to store medical information about a patient, with specific focus on storage of a child's vaccination history and safety.