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Artigos de revistas sobre o tema "Fluorimetry"

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Autor: Grafiati

Publicado: 4 de junho de 2021

Última modificação: 3 de março de 2023

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1

Thakur, Shikha, Neha Bajwa e Ashish Baldi. "NEW ANALYTICAL METHODS FOR ESTIMATION OF ARTEETHER BY UV AND FLUORESCENCE SPECTROPHOTOMETRY: DEVELOPMENT AND VALIDATION". Journal of Drug Delivery and Therapeutics 8, n.º 5 (15 de setembro de 2018): 151–58. http://dx.doi.org/10.22270/jddt.v8i5.1797.

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The present research work discusses the development and validation of two different spectrophotometric methods for estimation of Î±-Î² arteether using UV spectrophotometer and spectrofluorimeter for the first time. Two simple, accurate, precise, sensitiveand economical methods has been developed and validated for the estimation of Î±-Î² arteether in bulk and pharmaceutical dosage form as per ICH guidelines Q2(R1). The solvent used for UV spectroscopy was methanol and HCl (8:2) and methanol was used for fluorimeter. For qualitative and quantitative analysis, 254 nm was used in UV spectroscopy and excitation and emission wavelengths were set at 354 nm and 697 nm respectively for fluorimetry. Coefficients of correlation were found to be 0.993 and 0.992 for UV spectroscopy and fluorimetry. Both methods show good accuracy and precision and were compared statistically by using two way ANOVA which shows no significant difference between these methods. So the proposed methods were found to have equal applicability for estimation and routine analysis of arteether in pharmaceutical formulations. Keywords: Arteether, Analytical method, Fluorimeter, Pharmaceutical formulation, UV spectrophotometer.

2

Smith, B. W., B. T. Jones e J. D. Winefordner. "High-Precision Fluorimetry with a Light-Emitting Diode Source". Applied Spectroscopy 42, n.º 8 (novembro de 1988): 1469–72. http://dx.doi.org/10.1366/0003702884429535.

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A simple fluorimeter which uses a light-emitting diode as a source of excitation and a photodiode as a detector has been constructed and evaluated. The exceptional stability of both the detector and the source make it possible to make concentration measurements by fluorimetry with a precision of at least 0.002%. Furthermore, the system exhibited excellent linear dynamic range and a limit of detection of 20 ng/L for the dye Oxazine 720 in methanol.

3

Westerlund-Karlsson, Annette, Katriina Suonpää, Matti Ankelo, Jorma Ilonen, Mikael Knip e Ari E. Hinkkanen. "Detection of Autoantibodies to Protein Tyrosine Phosphatase-like Protein IA-2 with a Novel Time-resolved Fluorimetric Assay". Clinical Chemistry 49, n.º 6 (1 de junho de 2003): 916–23. http://dx.doi.org/10.1373/49.6.916.

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Abstract Background: Circulating autoantibodies to pancreatic glutamic acid decarboxylase (GAD65; the 65-kDa isoform of glutamic acid decarboxylase), protein tyrosine phosphatase-like protein IA-2, and insulin can be used as predictive markers of type 1 diabetes. We developed a novel assay for the detection of IA-2 autoantibodies (IA-2As) in serum based on time-resolved fluorimetry, hypothesizing that this kind of assay could provide several advantages over methods described to date, including radiobinding assays (RBAs) and ELISAs. Methods: The intracellular part of IA-2 (IA-2ic) was biotinylated and bound to streptavidin-coated 96-well plates by simultaneous incubation with serum samples and glutathione S-transferase (GST)-IA-2ic fusion protein. GST-IA-2ic captured by autoantibodies in the serum was detected with europium-labeled anti-GST antibody, and the signal was measured in a time-resolved fluorimeter. A serum sample panel from 100 patients with newly diagnosed type 1 diabetes and 100 unaffected controls was analyzed with the new assay and a conventional RBA. Results: Among the 100 serum samples from patients with type 1 diabetes, the time-resolved fluorimetric assay identified 74 IA-2A-containing sera, whereas the RBA detected 80 IA-2A-positive samples. Five of the six samples positive in the RBA but not detected by the time-resolved fluorimetric assay were only weakly positive in the RBA. The performance time of the time-resolved fluorimetric assay was 2.5 h compared with 10–12 h required by the RBA. Conclusions: The time-resolved fluorimetric assay provides a simple, nonradioactive analysis method for the detection of IA-2As with a specificity and a sensitivity comparable to the RBA method. This assay allows substantial reduction in performance time compared with the conventional RBA.

4

Valcárcel, M., A. Gómez-Hens e S. Rubio. "Simultaneous determination of epinephrine and norepinephrine in urine by derivative synchronous fluorescence spectroscopy." Clinical Chemistry 31, n.º 11 (1 de novembro de 1985): 1790–94. http://dx.doi.org/10.1093/clinchem/31.11.1790.

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Abstract The combination of synchronous and derivative fluorimetry, potentially useful in resolving mixtures that present strongly overlapping conventional fluorescence spectra, appears suitable for determining epinephrine and norepinephrine in urine. We describe a simple, rapid, and inexpensive method for simultaneous assay of these compounds in a single scan of urine specimens from normal adults. The catecholamines are extracted from the sample on cation-exchange columns and subsequently determined by formation of trihydroxyindoles. Analytical recovery is about 94% for epinephrine, 91% for norepinephrine, with a CV of approximately 5%. No sophisticated detection equipment is necessary, a conventional modern fluorimeter being adequate.

5

Neher, Erwin. "Quantitative Aspects of Calcium Fluorimetry". Cold Spring Harbor Protocols 2013, n.º 10 (outubro de 2013): pdb.top078204. http://dx.doi.org/10.1101/pdb.top078204.

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6

Fidanza, Joëlle, e Jean-Jacques Aaron. "Evaluation of filter papers as substrates for solid-surface room-temperature fluorimetry and photochemical fluorimetry". Talanta 33, n.º 3 (março de 1986): 215–18. http://dx.doi.org/10.1016/0039-9140(86)80054-6.

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7

Lesher-Pérez, Sasha Cai, Ge-Ah Kim, Chuan-hsien Kuo, Brendan M. Leung, Sanda Mong, Taisuke Kojima, Christopher Moraes, M. D. Thouless, Gary D. Luker e Shuichi Takayama. "Dispersible oxygen microsensors map oxygen gradients in three-dimensional cell cultures". Biomaterials Science 5, n.º 10 (2017): 2106–13. http://dx.doi.org/10.1039/c7bm00119c.

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8

Velmurugan, K., A. Raman, Derin Don, Lijun Tang, S. Easwaramoorthi e R. Nandhakumar. "Quinoline benzimidazole-conjugate for the highly selective detection of Zn(ii) by dual colorimetric and fluorescent turn-on responses". RSC Advances 5, n.º 55 (2015): 44463–69. http://dx.doi.org/10.1039/c5ra04523a.

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9

Askin, Samuel, Thomas E. H. Bond, Alanna E. Sorenson, Morgane J. J. Moreau, Helma Antony, Rohan A. Davis e Patrick M. Schaeffer. "Selective protein unfolding: a universal mechanism of action for the development of irreversible inhibitors". Chemical Communications 54, n.º 14 (2018): 1738–41. http://dx.doi.org/10.1039/c8cc00090e.

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10

Sadeghi, Susan, e Samieh Oliaei. "Optimization of ionic liquid based dispersive liquid–liquid microextraction combined with dispersive micro-solid phase extraction for the spectrofluorimetric determination of sulfasalazine in aqueous samples by response surface methodology". RSC Advances 6, n.º 114 (2016): 113551–60. http://dx.doi.org/10.1039/c6ra20223c.

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11

Summers, H. D., e P. A. Porta. "Resonant-cavity optoelectronic devices for fluorimetry". IEEE Journal of Selected Topics in Quantum Electronics 11, n.º 4 (julho de 2005): 854–57. http://dx.doi.org/10.1109/jstqe.2005.857740.

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12

Swatland, H. J. "Fluorimetry of Gelatin by Fiber Optics". Canadian Institute of Food Science and Technology Journal 20, n.º 2 (abril de 1987): 102–3. http://dx.doi.org/10.1016/s0315-5463(87)71098-0.

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13

Roblin, G., L. Bernstein, M. Prevost e G. Slucki. "Scanning optical microscopy applied to fluorimetry". Journal of Optics 17, n.º 6 (novembro de 1986): 259–69. http://dx.doi.org/10.1088/0150-536x/17/6/001.

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14

Bergmann, Stephan, Viola Mönkemöller e Thomas Huser. "Comment on “Magnetic-field-enabled resolution enhancement in super-resolution imaging” by M. Zhang et al., Phys. Chem. Chem. Phys., 2015, 17, 6722–6727". Physical Chemistry Chemical Physics 19, n.º 6 (2017): 4887–90. http://dx.doi.org/10.1039/c6cp05108a.

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15

Fagadar-Cosma, Eugenia, Nicoleta Plesu, Anca Lascu, Diana Anghel, Maria Cazacu, Catalin Ianasi, Gheorghe Fagadar-Cosma, Ion Fratilescu e Camelia Epuran. "Novel Platinum-Porphyrin as Sensing Compound for Efficient Fluorescent and Electrochemical Detection of H2O2". Chemosensors 8, n.º 2 (23 de abril de 2020): 29. http://dx.doi.org/10.3390/chemosensors8020029.

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Metalloporphyrins are highly recognized for their capacity to act as sensitive substances used in formulation of optical, fluorescent, and electrochemical sensors. A novel compound, namely Pt(II)-5,10,15,20-tetra-(4-allyloxy-phenyl) porphyrin, was synthesized by metalation with PtCl2(PhCN)2 of the corresponding porphyrin base and was fully characterized by UV-vis, fluorimetry, FT-IR, 1H-NMR, and 13C-NMR methods. The fluorescence response of this Pt-porphyrin in the presence of different concentrations of hydrogen peroxide was investigated. Besides, modified glassy carbon electrodes with this Pt-porphyrin (Pt-Porf-GCE) were realized and several electrochemical characterizations were comparatively performed with bare glassy carbon electrodes (GCE), in the absence or presence of hydrogen peroxide. The Pt-porphyrin demonstrated to be a successful sensitive material for the detection of hydrogen peroxide both by fluorimetric method in a concentration range relevant for biological samples (1.05–3.9 × 10−7 M) and by electrochemical method, in a larger concentration range from 1 × 10−6 M to 5 × 10−5 M. Based on different methods, this Pt-porphyrin can cover detection in diverse fields, from medical tests to food and agricultural monitoring, proving high accuracy (correlation coefficients over 99%) in both fluorimetric and electrochemical measurements.

16

Shalan, Shereen, e Jenny Jeehan Nasr. "Simultaneous evaluation of losartan and amlodipine besylate using second-derivative synchronous spectrofluorimetric technique and liquid chromatography with time-programmed fluorimetric detection". Royal Society Open Science 6, n.º 4 (abril de 2019): 190310. http://dx.doi.org/10.1098/rsos.190310.

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This study is concerned with two sensitive, fast and reproducible approaches; namely, second-derivative synchronous fluorimetry (method I) and reversed phase high-performance liquid chromatography with fluorimetric detection (method II) for synchronized evaluation of losartan (LOS) and amlodipine besylate (AML). Method I is based on measuring second-derivative synchronous fluorescence spectra of LOS and AML at Δ λ = 80 nm in water. The experimental factors influencing the synchronous fluorescence of the considered compounds were sensibly adjusted. The chromatographic analysis was executed on a Nucleodur MN-C18 column of dimensions; 250 × 4.6 mm i.d. and 5 µm particle size). The fluorimetric detection was time-programmed at λ em = 440 nm for AML (0.0–7.5 min) and at λ em = 400 nm for LOS (7.5–10 min) after excitation at λ ex = 245 nm. The mobile phase is a blend of acetonitrile with 0.02 M phosphate buffer in a proportion of 45 : 55, pH 4.0, pumped using a flow rate of 1 ml min −1 . The calibration plots were established to be 0.1–4.0 µg ml −1 for both drugs in method I and 0.05–4.0 µg ml −1 for both drugs in method II. The study was extended to the evaluation of the two drugs in their co-formulated tablets.

17

Lu, Fei, Jing-Hao Pan, Yun Liu, Hongfen Zhang, Yujing Guo e Yingte Wang. "Study on the interaction of bovine serum albumin with acid cyanine 5R and its application in analysis". Biochemistry and Cell Biology 84, n.º 1 (1 de fevereiro de 2006): 1–8. http://dx.doi.org/10.1139/o05-051.

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A supramolecular complex of bovine serum albumin (BSA) with acid cyanine 5R (AC 5R, C.I. acid blue 113, C.I.: 26360) has been shown to form in Tris–HCl buffer solution (pH 7.42) by linear sweep voltammetry (LSV), fluorimetry, and spectrophotometry. The binding ratio and binding constant of BSA with AC 5R have been detected by LSV and fluorimetry. The binding mechanism is also preliminarily discussed. In Tris–HCl buffer solution (pH 7.42), AC 5R can easily be reduced on the mercury electrode, and it has a well-defined LSV peak current (Ip) and peak potential (Ep) at –0.65 V (vs. SCE). In the presence of BSA, the Ipof AC 5R decreases, and the peak potential (Ep) shifts to a more positive potential. The decrease of the second-order derivative of reductive peak current (ΔIp") of AC 5R is proportional to the logarithm of BSA concentration in the range of 1.54 × 10–8mol·L–1– 1.54 × 10–5mol·L–1(r = 0.9931 – 0.9977). The limit of detection of BSA is 9.0 × 10–9mol·L–1. The relative standard deviation is 1.83% (n = 10), and the standard recovery is 97.5%–104.8%. This method can be used to determine BSA concentration on the basis of the interaction of BSA with AC 5R.Key words: bovine serum albumin (BSA), acid cyanine 5R (AC 5R), supramolecule, binding ratio, binding constant, fluorimetry, spectrophotometry, electroanalytical method.

18

Cao, Lina, Hongyong Xiang, Jingwen Xu, Yufu Gao, Chenlu Lin, Kun Li, Zhiwei Li et al. "Nutrient Detection Sensors in Seawater Based on ISI Web of Science Database". Journal of Sensors 2022 (7 de março de 2022): 1–12. http://dx.doi.org/10.1155/2022/5754751.

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Marine ecosystem is increasingly deteriorating. In order to assess anthropogenic influence and instigate appropriate remedial actions, it is still of great significance to develop the technology of sensors applied for nutrient detection (e.g., nitrate, phosphate, and silicate) in seawater. This brief review shows an important direction for the development of nutrient detection sensors in seawater and also the limitations and challenges based on data from the ISI Web of Science database. Being different from previous review papers, in this short critical review paper (1) we unified the unit of limit of detection (LOD) for making the comparison within different researches possible; (2) only the literatures focusing on the technological development of sensors in seawater were used; and (3) not only the detection methods but also the detected analytes and publication years were discussed to supply more valuable information for the development of nutrient sensors applied in seawater. In total, 109 literatures were collected with regard to technological development. The quantity of literatures has increased most during 2011-2020. For analytes, literatures related to nitrate, phosphate, ammonium, and phosphate will continue to increase with more accurate data. For detection methods, spectrophotometry, colorimetry, fluorimetry, and electrochemistry are the most widely used sensors. LODs show thousands of orders. In general, there are lower LOD to nitrite and ammonium and fluorimetry method. Now, for analytes, nitrate 1.0983 > silicate 0.5495 > phosphate 0.4823 > ammonium 0.1324 > nitrite 0.0568 . For detection methods, microfluidics 1.7617 > electrochemistry 1.2607 > colorimetry 0.4462 > spectrophotometry 0.2941 > fluorimetry 0.0558 . This result indicated that the development level of detection methods is closer for nitrate, nitrite, phosphate, and silicate. For ammonium, spectrophotometry has significantly lower LOD than electrochemistry ( p < 0.05 ), and fluorimetry also has significantly lower LOD than electrochemistry ( p < 0.05 ). Our results imply that sensors with accurate LOD should be developed in the future. In addition, more detection methods should be considered by future sensors.

19

Shao, Hao, Keely Oltion, Taia Wu e Jason E. Gestwicki. "Differential scanning fluorimetry (DSF) screen to identify inhibitors of Hsp60 protein–protein interactions". Organic & Biomolecular Chemistry 18, n.º 22 (2020): 4157–63. http://dx.doi.org/10.1039/d0ob00928h.

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Homo-oligomers are difficult drug targets. Here, Differential Scanning Fluorimetry (DSF) is introduced as a method to identify inhibitors of these systems, by discriminating between oligomers and monomers based on their thermal stability.

20

Gao, Kai, Shabnam Shaabani, Ruixue Xu, Tryfon Zarganes-Tzitzikas, Li Gao, Maryam Ahmadianmoghaddam, Matthew R. Groves e Alexander Dömling. "Nanoscale, automated, high throughput synthesis and screening for the accelerated discovery of protein modifiers". RSC Medicinal Chemistry 12, n.º 5 (2021): 809–18. http://dx.doi.org/10.1039/d1md00087j.

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Acoustic dispensing was used to synthesize 1536 compounds on a nano scale, screened by differential scanning fluorimetry, cross-validated by microscale thermophoresis against the protein interaction menin–MLL to discover binders, one co-crystallized.

21

Fayed, Ahmed S., Maha A. Hegazy, Enas E. Abbas e Nahla N. Salama. "Rapid and selective determination of pitavastatin calcium in presence of its degradation products and co-formulated drug by first-derivative micelle-enhanced and synchronous fluorimetric methods". RSC Advances 6, n.º 109 (2016): 107246–55. http://dx.doi.org/10.1039/c6ra20813d.

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New, selective and rapid methods are presented for determination of PIT in the presence of its hydrolytic degradation products and co-formulated drug, EZE. These methods are derivative micelle enhanced native fluorescence and synchronous fluorimetry.

22

Li, Gui-Rong, Meng-Yuan Xu, Jie-Kang Li e Yang Yang. "A study on the preparation and application of a core–shell surface imprinted uranyl magnetic chelating adsorbent". RSC Advances 8, n.º 65 (2018): 37401–9. http://dx.doi.org/10.1039/c8ra06992a.

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A core shell UMCA was synthesized using surface imprinting technique. UMCA was used for the determination of uranyl by SPE coupled with fluorimetry. This as-prepared UMCA exhibits high adsorption capacity, good extraction performance and stability.

23

Kamochkina, I. Ya, Ye M. Rekharsky, A. P. Chukharkhina e A. G. Borzenko. "Pefloxacin determination in urine by synchronous fluorimetry". Moscow University Chemistry Bulletin 62, n.º 2 (abril de 2007): 81–84. http://dx.doi.org/10.3103/s0027131407020058.

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24

Levin, A. D. "Metrological Support to Fluorimetry on Biological Molecules". Measurement Techniques 47, n.º 5 (maio de 2004): 500–504. http://dx.doi.org/10.1023/b:mete.0000038120.57051.0f.

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25

Chuan, Dong, Yuan Wen e Yang Pin. "Investigation on Thioguanine by Solid Surface Fluorimetry". Analytical Letters 33, n.º 14 (janeiro de 2000): 2939–50. http://dx.doi.org/10.1080/00032710008543232.

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26

Feddersen, Brett A., David W. Piston e Enrico Gratton. "Digital parallel acquisition in frequency domain fluorimetry". Review of Scientific Instruments 60, n.º 9 (setembro de 1989): 2929–36. http://dx.doi.org/10.1063/1.1140629.

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27

Agafonova, N. A., G. A. Sakuta, Yu M. Rozanov, G. I. Shtein e B. N. Kudryavtsev. "DNA image-fluorimetry of individual human chromosomes". Cell and Tissue Biology 7, n.º 4 (julho de 2013): 352–61. http://dx.doi.org/10.1134/s1990519x13040020.

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28

Meng, Jian-Xin, He-Jun Wu e De-Xiong Feng. "Fluorimetry determination of trace Ce3+ with EDTP". Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy 56, n.º 10 (setembro de 2000): 1925–28. http://dx.doi.org/10.1016/s1386-1425(00)00251-1.

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29

Wang, H. "Determination of gentamycin by synchronous derivative fluorimetry". Talanta 52, n.º 2 (21 de junho de 2000): 201–9. http://dx.doi.org/10.1016/s0039-9140(00)00335-0.

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30

Braun, T., e Agnes Klein. "Shpol'skii fluorimetry: The anatomy of an eponym". TrAC Trends in Analytical Chemistry 11, n.º 6 (junho de 1992): 200–202. http://dx.doi.org/10.1016/0165-9936(92)80042-5.

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31

Kawabata, Yuji, Totaro Imasaka e Nobuhiko Ishibashi. "Ultramicro flow-cell for semiconductor laser fluorimetry". Talanta 33, n.º 3 (março de 1986): 281–83. http://dx.doi.org/10.1016/0039-9140(86)80066-2.

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32

Sanchez, F. "Trace zinc determination by synchronous derivative fluorimetry". Talanta 33, n.º 10 (outubro de 1986): 785–89. http://dx.doi.org/10.1016/0039-9140(86)80194-1.

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33

Petrea, R. "Fiber-Optic time-resolved fluorimetry for immunoassays". Talanta 35, n.º 2 (fevereiro de 1988): 139–44. http://dx.doi.org/10.1016/0039-9140(88)80052-3.

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34

Silvers, Robert, Heiko Keller, Harald Schwalbe e Martin Hengesbach. "Differential Scanning Fluorimetry for Monitoring RNA Stability". ChemBioChem 16, n.º 7 (13 de abril de 2015): 1109–14. http://dx.doi.org/10.1002/cbic.201500046.

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35

Yu, Yongli, Jianli Niu, Wei Li, Haixia Liu e Meng Wang. "Sensitive detection of Brilliant Blue using fluorescence resonance energy transfer with YVO4:Eu nanocrystals as donors". Analytical Methods 10, n.º 3 (2018): 359–64. http://dx.doi.org/10.1039/c7ay02281f.

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A FRET system was established using YVO₄:Eu nanocrystals (NCs) as the energy donors and Brilliant Blue (BB) as the energy acceptor. Based on FRET, fluorimetry was successfully applied to determine BB in juice and candy samples.

36

Jarvie, H. P., A. J. Love, R. J. Williams e C. Neal. "Measuring in-stream productivity: the potential of continuous chlorophyll and dissolved oxygen monitoring for assessing the ecological status of surface waters". Water Science and Technology 48, n.º 10 (1 de novembro de 2003): 191–98. http://dx.doi.org/10.2166/wst.2003.0573.

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Continuous (hourly) measurements of dissolved oxygen and chlorophyll (determined by fluorimetry) were made for an inter-linked lowland river and canal system. The dissolved oxygen data were used to estimate daily rates of re-aeration, photosynthesis and respiration, using a process-based analytical technique (the Delta method). In-situ fluorimeter measurements of chlorophyll were ground-truthed on a fortnightly basis using laboratory methanol extraction of chlorophyll and spectrophotometric analysis. Water samples were also analysed for algal species on a fortnightly basis. The river and canal exhibited very similar rates of photosynthesis and respiration during the summer of 2001, despite much higher chlorophyll concentrations and total algal counts, indicating that benthic algae and/or aquatic macrophytes may be making an important contribution to photosynthesis rates in the river. Suspended algal populations in the canal are dominated by planktonic species, whereas the river has a higher proportion of species which are predominantly benthic in habitat. The river exhibited higher rates of respiration, reflecting a higher organic loading from external (e.g. sewage effluent) sources.

37

Janssen, Luke J., Pierre A. Betti, Stuart J. Netherton e Denise K. Walters. "Superficial buffer barrier and preferentially directed release of Ca2+ in canine airway smooth muscle". American Journal of Physiology-Lung Cellular and Molecular Physiology 276, n.º 5 (1 de maio de 1999): L744—L753. http://dx.doi.org/10.1152/ajplung.1999.276.5.l744.

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We examined cytosolic concentration of Ca2+([Ca2+]i) in canine airway smooth muscle using fura 2 fluorimetry (global changes in [Ca2+]i), membrane currents (subsarcolemmal [Ca2+]i), and contractions (deep cytosolic [Ca2+]i). Acetylcholine (10−4 M) elicited fluorimetric, electrophysiological, and mechanical responses. Caffeine (5 mM), ryanodine (0.1–30 μM), and 4-chloro-3-ethylphenol (0.1–0.3 mM), all of which trigger Ca2+-induced Ca2+ release, evoked Ca2+ transients and membrane currents but not contractions. The sarcoplasmic reticulum (SR) Ca2+-pump inhibitor cyclopiazonic acid (CPA; 10 μM) evoked Ca2+transients and contractions but not membrane currents. Caffeine occluded the response to CPA, whereas CPA occluded the response to acetylcholine. Finally, KCl contractions were augmented by CPA, ryanodine, or saturation of the SR and reduced when SR filling state was decreased before exposure to KCl. We conclude that 1) the SR forms a superficial buffer barrier dividing the cytosol into functionally distinct compartments in which [Ca2+]iis regulated independently; 2) Ca2+-induced Ca2+ release is preferentially directed toward the sarcolemma; and 3) there is no evidence for multiple, pharmacologically distinct Ca2+ pools.

38

Pradhan, Susanta Kumar, e Balram Ambade. "Determination of trace uranium in thorium matrix by laser induced fluorimetry after separation of thorium by its fluoride precipitation using NH4HF2". Radiochimica Acta 109, n.º 3 (26 de janeiro de 2021): 195–203. http://dx.doi.org/10.1515/ract-2020-0050.

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Abstract Thorium, a major element in thorium matrix, quenches uranium fluorescence when it is present above the ratio (Th/U) of 2000 in conventional pellet fluorimetry determination of uranium. A single step ‘sample digestion cum thorium fluoride precipitation’ with NH4HF2 has been developed for separation of bulk thorium as hydrated thorium fluoride precipitates. Uranium in aqueous solution is extracted into ethyl acetate and stripped into pyrophosphate medium (pH ∼ 7), prior to its laser induced fluorimetry determination. Optimizations of certain parameters such as the effects of fluoride flux, mineral acid, temperature and time, stripping solution, diverse ions etc. are discussed in detail. The method has been validated by analyzing a set of synthetic mixtures and certified reference materials of rock samples such as SY-2, SY-3, GSP-2, NKT-1 and CG-2 doped with a large excess of thorium. This method has been applied for the determination of microgram to nanogram uranium in thorium rich rocks and synthetic nuclear grade ThO2 with high degree of accuracy and precision. This is the improvement of the existing method which involves two liquid-liquid solvent extraction separation of thorium and uranium using the chelating agent 2,3-dihydroxynaphthalene at the different pH, compared to one solvent extraction separation of uranium in the present method, because separation of thorium by precipitation as its fluoride has already been carried out during sample digestion step itself. The proposed method involving ammonium hydrogen fluoride in combination with laser induced fluorimetry is simple, rapid, cost effective and more eco-friendly.

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Abdourasmane Kadougoudiou Konate, Adama Zongo, Jean Rodrigue Sangaré, Audrey Dardou e Alain Audebert. "High-throughput phenotyping for drought tolerance in rice". World Journal of Advanced Research and Reviews 12, n.º 2 (30 de novembro de 2021): 379–91. http://dx.doi.org/10.30574/wjarr.2021.12.2.0597.

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Most lowland rice in West Africa depends mainly on rainfall for water supply. Drought is consequently one of the major constraints on rice production, drastically affecting both plant growth and development. The objective of this work was to study the impact of water deficit both on canopy temperature and on chlorophyll fluorescence level, used as indicators of transpiration and photosynthetic activity. Measurements using infrared thermography and fluorimetry were taken on both 17 lines resulting from the cross IR64 X B6144F-MR-6-0-0 and their two parents plus one tolerant (APO) controls. These 20 lines were phenotyped after applying a drought constraint in a controlled laboratory environment in Montpellier (France) in 2013 and - 2014 and in field in the lowlands of Banfora and Farako-ba (INERA Burkina Faso) in 2014. Results showed that the drought stress sustained by the plants increased canopy temperature in all lines, entailing differential disturbance of the photosynthetic process, markedly depressed in susceptible lines. A classification of the lines with respect to their sensitivity to stress could be established by using the Drought Factor Index (DFI), and Crop Water Stress Index (CWSI) as was established a correlation between the phenotyping methods by infrared thermography and fluorimetry. This article propose an efficient application of combined imaging as a rapid and accurate phenotyping tool for crop yield improvement, in particular by monitoring the efficiency of plant responses to the fluctuating of environmental conditions. This study proved the efficiency of the method combining IR thermographie and fluorimetry as a field phenotyping tools for drought resistance.

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Shimkevich, Andrey M., V. N. Makarov, Inessa M. Goloenko e Oleg G. Davydenko. "Functional State Of Photosynthetic System In Barley Alloplasmic Lines". Ecological genetics 4, n.º 2 (15 de junho de 2006): 37–42. http://dx.doi.org/10.17816/ecogen4237-42.

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The peculiarities of photosynthetic system functioning in barley alloplasmic lines were investigated by PAM fluorimetry. The substitution of nuclear genome was shown to affect differently several photosynthetic parameters. The importance of the balance between nuclear and organelle barley genetic systems for the photosystem II functioning was demonstrated.

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Yagüe, Paula, Angel Manteca, Alejandro Simon, Marta Elena Diaz-Garcia e Jesus Sanchez. "New Method for Monitoring Programmed Cell Death and Differentiation in Submerged Streptomyces Cultures". Applied and Environmental Microbiology 76, n.º 10 (26 de março de 2010): 3401–4. http://dx.doi.org/10.1128/aem.00120-10.

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ABSTRACT Vital stains were used in combination with fluorimetry for the elaboration of a new method to quantify Streptomyces programmed cell death, one of the key events in Streptomyces differentiation. The experimental approach described opens the possibility of designing online protocols for automatic monitoring of industrial fermentations.

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Coremans, Joanna M. C. C., Matthijs Van Aken, Daniëlle C. W. H. Naus, Marie-Louise F. Van Velthuysen, Hajo A. Bruining e Gerwin J. Puppels. "Pretransplantation assessment of renal viability with NADH fluorimetry". Kidney International 57, n.º 2 (outubro de 2000): 671–83. http://dx.doi.org/10.1046/j.1523-1755.2000.00889.x.

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Bohn, Matthew J. "Frequency domain fluorimetry using a mercury vapor lamp". Journal of Applied Remote Sensing 3, n.º 1 (1 de abril de 2009): 033524. http://dx.doi.org/10.1117/1.3117448.

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Guo, X. Q., J. G. Xu, X. Z. Huang, Y. B. Zhao e G. Z. Chen. "Determination of Biacetyl by Sensitized Photochemical Kinetic Fluorimetry". Analytical Letters 26, n.º 8 (agosto de 1993): 1693–710. http://dx.doi.org/10.1080/00032719308021490.

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Wang, Huai You, Lian Dong Liu e Jian Cheng Ren. "Determination of methyl isocyanate in air by fluorimetry". Analyst 124, n.º 9 (1999): 1327–30. http://dx.doi.org/10.1039/a903014j.

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Veselsky, Johannes C., Barbara Kwiecinska, Edith Wehrstein e Otto Suschny. "Determination of uranium in minerals by laser fluorimetry". Analyst 113, n.º 3 (1988): 451. http://dx.doi.org/10.1039/an9881300451.

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Imasaka, Totaro, Takashi Okazaki e Nobuhiko Ishibashi. "Semiconductor laser fluorimetry for enzyme and enzymatic assays". Analytica Chimica Acta 208 (1988): 325–29. http://dx.doi.org/10.1016/s0003-2670(00)80765-5.

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Grahn, M. F., M. L. De Jode, M. G. Dilkes, J. K. Ansell, D. Onwu, J. Maudsley e N. S. Williams. "Tissue photosensitizer detection by low-power remittance fluorimetry". Lasers in Medical Science 12, n.º 3 (outubro de 1997): 245–52. http://dx.doi.org/10.1007/bf02765105.

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Puchalski, M. M., M. J. Morra e R. von Wandruszka. "Assessment of inner filter effect corrections in fluorimetry". Fresenius' Journal of Analytical Chemistry 340, n.º 6 (junho de 1991): 341–44. http://dx.doi.org/10.1007/bf00321578.

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Dou, Ying, Zeineb Es-Salah-Lamoureux, Ping Yu Xiong e David Fedida. "Understanding hERG Channels Gating using Voltage-Clamp Fluorimetry". Biophysical Journal 102, n.º 3 (janeiro de 2012): 329a—330a. http://dx.doi.org/10.1016/j.bpj.2011.11.1806.

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