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Artigos de revistas sobre o tema "Culture"

Autor: Grafiati

Publicado: 4 de junho de 2021

Última modificação: 16 de novembro de 2024

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1

Sullivan, Patrick. "Introduction: Culture Without Cultures-The Culture Effect". Australian Journal of Anthropology 17, n.º 3 (dezembro de 2006): 253–64. http://dx.doi.org/10.1111/j.1835-9310.2006.tb00062.x.

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Waque, Victor. "Projet « La culture, les cultures, ma culture »". L'Education physique en mouvement, n.º 6 (18 de dezembro de 2022): 29–30. http://dx.doi.org/10.26034/vd.epm.2021.3534.

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Dans le lycée professionnel Arthur Rimbaud, à Garges-lès-gonesse, en France, deux classes se sont engagées sur une projet interdisciplinaire (éducation physique, français, histoire) autour de la notion de culture. L’aventure s’est finalisée par un clip de danse accompagné des textes rédigés par les élèves sur leur culture. Victor Waque (VW), l’un des enseignants responsable de ce projet a accepté de répondre à nos questions et de nous fournir un retour d’expérience.

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Souchand, Jean-François. "Culture et Cultures". Journal of French and Francophone Philosophy 5, n.º 2-3 (3 de março de 1993): 44–57. http://dx.doi.org/10.5195/jffp.1993.75.

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Roerich, Nicholas. "On Culture, Values, and Peace (Selected Essays and Letters)". Labyrinth 20, n.º 2 (15 de março de 2019): 171. http://dx.doi.org/10.25180/lj.v20i2.141.

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1. The Banner of Peace2. The Sacred Sign of Peace3. The Heart of Culture4. The Red Cross of Culture5. The Mission of Womanhood6. Glory to Women, Bearers of Culture7. Roots of Culture8. In spite of difficulties (To the Bulgarian Society, 1930)9. Re-evaluation10. Defense of Values

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González Delgado, Ángel Adrián. "Cultura de Paz y Cultura de la argumentación. Una aproximación al posible vínculo entre ambas culturas". Miscelánea Filosófica αρχή Revista Electrónica 7, n.º 20 (29 de dezembro de 2023): 37–53. http://dx.doi.org/10.31644/mfarchere_v.7;n.20/24-a02.

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Culture of peace and argumentation culture is the general topic of this article. The principal objective is to propose the question about a possible relation between both cultures. To achieves this objective, we going to review the definition of culture of peace from the UNESCO. In the same way, we going to defined argumentation culture and proposed some thoughts about it.

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NAWA, Kotaro. "Two cultures, or S1 culture and S2 culture". Journal of Information Processing and Management 46, n.º 7 (2003): 475–77. http://dx.doi.org/10.1241/johokanri.46.475.

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Hodge, Kyle, e Jenna Khan. "Residual Component Culture: Application of Standardized Transfusion Reaction Culture Criteria to Reduce Culture Rates". American Journal of Clinical Pathology 160, Supplement_1 (1 de novembro de 2023): S122. http://dx.doi.org/10.1093/ajcp/aqad150.266.

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Abstract Blood products are often cultured as part of a transfusion reaction (TRXN) workup in order to “not miss” a septic TRXN. These cultures are vulnerable to secondary contamination and are often challenging to interpret due to the lack of a corresponding patient blood culture. Although bacterial contamination of blood products remains a leading cause of transfusion related fatalities, the actual prevalence of primary contamination of blood components is quite low, leading to a low positive predictive value (PPV) for component culture. Given recently mandated mitigation strategies to reduce bacterial contamination of platelets, the PPV of component culture is likely to further decline, making appropriate selection criteria for culture even more critical to conserve laboratory resources and maximize clinical utility. At our institution the decision to culture is at the discretion of the pathology resident and/or transfusion medicine physician. We sought to review our current culture rates and determine if applying standardized indications for component culture could reduce culture rates without impacting diagnostic utility. Clinical symptoms and vital signs were reviewed for the 24-hour period after the reaction for all TRXNs reported from July 2021 to June 2022. Of the 105 reactions reported during the study timeframe, 39 (37%) were cultured, with one positive culture involving a platelet unit. This was deemed a true septic reaction given concordant cultures in the patient. Modified forms of the AABB and BEST culture criteria were then applied to the reported reactions. Modifications were made to provide specific definitions for tachycardia (heart rate > 100bpm and at least 15% increase) and hypotension (SBP < 80mmhg and at least a 30mmHg decrease). Application of the AABB criteria reduces the number of cultures to 22 (21% of reactions) whereas the BEST criteria increases the number of cultures to 51 (49% of reactions). Both criteria captured the true positive septic reaction. Neither criteria were completely concordant with our current practice. Of the 39 reactions selected for culture by our institution, 26 (67%) did not meet AABB criteria and 11 (28%) did not met BEST criteria; 9 and 22 different reactions would have been cultured based on AABB and BEST criteria respectively. Although we are not able to confirm if culturing of the discrepant units would have yielded additional positive cultures, retrospective chart review did not reveal any additional cases of clinically significant sepsis. The modified AABB criteria decreased the culture rate by 56% while still capturing the true positive septic TRXN suggesting that it would be the best option to reduce the cost/burden of culturing products without reducing diagnostic accuracy. Both published criteria do not take into consideration duration of symptoms and clinical interventions, thus clinical judgement remains critical to the decision to culture.

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Pena-ruiz, Henri. "Culture, cultures, et laïcité". Hommes et Migrations 1259, n.º 1 (2006): 6–16. http://dx.doi.org/10.3406/homig.2006.4414.

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Melero De la Torre, Mariano C. "Cultura constitucional = Constitutional culture". EUNOMÍA. Revista en Cultura de la Legalidad, n.º 15 (1 de outubro de 2018): 224. http://dx.doi.org/10.20318/eunomia.2018.4352.

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Resumen: La “cultura constitucional” hace referencia a la Constitución “realizada” o “actualizada” en las prácticas, hábitos y actitudes de los poderes públicos y la ciudadanía en general de una comunidad política. Este artículo trata de explorar las formas de interdependencia de la norma constitucional y la realidad cultural, añadiendo al final algunas reflexiones sobre el papel de los jueces en un Estado constitucional. Palabras clave: Cultura constitucional, mutación constitucional, cultura de la autoridad, cultura de la justificación, cultura democrática de la justificación.Abstract: The “constitutional culture” means the “realization” or “actualization” of the Constitution in the practices, habits and attitudes of the public powers and the general citizenship of a political community. This paper tries to explore the forms of interdependence between the constitutional norm and the cultural reality, adding at the end some reflections about the role of judges in a constitutional State.Keywords: Constitutional culture, living tree, culture of authority, culture of justification, democratic culture of justification.

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Yorio, Patrick L., Jason Edwards e Dick Hoeneveld. "Safety culture across cultures". Safety Science 120 (dezembro de 2019): 402–10. http://dx.doi.org/10.1016/j.ssci.2019.07.021.

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Schiele, Bernard. "Cultures of Scientific Culture". Cultures of Science 1, n.º 1 (setembro de 2018): 15–23. http://dx.doi.org/10.1177/209660831800100103.

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The sciencescape has been completely transformed in just over 30 years. Science exerts such impact on today's society as to have completely remodelled it. The ways of appropriating knowledge, individual and collective, in terms of parcelling disciplines, the burgeoning knowledge produced by each of them, and the demultiplication of information sources, can no longer be thought of in terms of the model that prevailed up to now. To imagine another model, one must try to understand what has changed.

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A, Eisenberg. "How did the Cultural Revolution affect your Culture?" Journal of Natural & Ayurvedic Medicine 4, n.º 3 (6 de julho de 2020): 1–4. http://dx.doi.org/10.23880/jonam-16000270.

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While serving as International Expert at the Research Institute of Anthropology and Ethnology, Jishou University in Xiangxi Autonomous Prefecture of Hunan Province, China, on United Nations Educational, Scientific and Cultural Organization-Local and Indigenous Knowledge Systems (UNESCO-LINKS) Natural Science Sector, United Nations Permanent Forum on Indigenous Issues (UNPFII), United Nations Department of Economic and Social Affairs (UNDESA) projects with the Kam people of China and ministries responsible for ethnic development, I asked my ethnic minority graduate students and colleagues of China this question, “How did the Cultural Revolution affect your culture?”, and they thoughtfully shared their perspectives and experiences.

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Seregin, A. A. "Culture of Dialogue of Cultures: New Challenges of Modern Time". Contemporary problems of social work 6, n.º 1 (31 de março de 2020): 84–91. http://dx.doi.org/10.17922/2412-5466-2020-6-1-84-91.

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Agius, L. "Metabolic interactions of parenchymal hepatocytes and dividing epithelial cells in co-culture". Biochemical Journal 252, n.º 1 (15 de maio de 1988): 23–28. http://dx.doi.org/10.1042/bj2520023.

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When parenchymal hepatocytes isolated from adult liver are co-cultured with other epithelial cells, the production of various plasma proteins by the hepatocytes is preserved for much longer than in conventional culture. This study examines some of the metabolic interactions between parenchymal hepatocytes and epithelial cells maintained in co-culture. The leakage of lactate dehydrogenase by hepatocytes co-cultured with epithelial cells was lower than in conventional hepatocyte culture. The epithelial cells have a high glycolytic rate and provide the hepatocytes with a continual supply of lactate. The [lactate] was lower in co-cultures of hepatocytes and epithelial cells than in pure epithelial cultures of similar density, suggesting lactate clearance by the hepatocytes. Alanine uptake was higher in conventional hepatocyte cultures, which lack an exogenous supply of lactate, than in parenchymal hepatocytes in co-culture. Studies with pure parenchymal hepatocytes incubated with increasing [lactate] suggest that lactate is utilized in preference to alanine as a gluconeogenic substrate by hepatocytes co-cultured with epithelial cells. Ketogenesis and carnitine palmitoyltransferase activity declined more slowly in hepatocytes co-cultured with epithelial cells than in conventional culture. It is concluded that the co-culture model has potential for long-term studies of carbohydrate and lipid metabolism.

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15

Robin, Régine. "La culture, les cultures, ma culture, les pièges du culturalisme". Francophonies d'Amérique, n.º 10 (2000): 7. http://dx.doi.org/10.7202/1005077ar.

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Koh, Benson, Nadiah Sulaiman, Mh Busra Fauzi, Jia Xian Law, Min Hwei Ng, Too Lih Yuan, Abdul Ghani Nur Azurah, Mohd Heikal Mohd Yunus, Ruszymah Bt Hj Idrus e Muhammad Dain Yazid. "A Three-Dimensional Xeno-Free Culture Condition for Wharton’s Jelly-Mesenchymal Stem Cells: The Pros and Cons". International Journal of Molecular Sciences 24, n.º 4 (13 de fevereiro de 2023): 3745. http://dx.doi.org/10.3390/ijms24043745.

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Xeno-free three-dimensional cultures are gaining attention for mesenchymal stem cell (MSCs) expansion in clinical applications. We investigated the potential of xeno-free serum alternatives, human serum and human platelet lysate, to replace the current conventional use of foetal bovine serum for subsequent MSCs microcarrier cultures. In this study, Wharton’s Jelly MSCs were cultured in nine different media combinations to identify the best xeno-free culture media for MSCs culture. Cell proliferation and viability were identified, and the cultured MSCs were characterised in accordance with the minimal criteria for defining multipotent mesenchymal stromal cells by the International Society for Cellular Therapy (ISCT). The selected culture media was then used in the microcarrier culture of MSCs to determine the potential of a three-dimensional culture system in the expansion of MSCs for future clinical applications, and to identify the immunomodulatory potential of cultured MSCs. Low Glucose DMEM (LG) + Human Platelet (HPL) lysate media appeared to be good candidates for replacing conventional MSCs culture media in our monolayer culture system. MSCs cultured in LG-HPL achieved high cell yield, with characteristics that remained as described by ISCT, although the overall mitochondrial activity of the cells was lower than the control and the subsequent effects remained unknown. MSC microcarrier culture, on the other hand, showed comparable cell characteristics with monolayer culture, yet had stagnated cell proliferation, which is potentially due to the inactivation of FAK. Nonetheless, both the MSCs monolayer culture and the microcarrier culture showed high suppressive activity on TNF-α, and only the MSC microcarrier culture has a better suppression of IL-1 secretion. In conclusion, LG-HPL was identified as a good xeno-free media for WJMSCs culture, and although further mechanistic research is needed, the results show that the xeno-free three-dimensional culture maintained MSC characteristics and improved immunomodulatory activities, suggesting the potential of translating the monolayer culture into this culture system in MSC expansion for future clinical application.

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Chen, Dou. "A Cross-Cultural Analysis of Chinese Traditional Culture and American Culture Elements of the Movie of Guasha Treatment". International Journal of Languages, Literature and Linguistics 6, n.º 1 (março de 2020): 56–59. http://dx.doi.org/10.18178/ijlll.2020.6.1.250.

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Elliott, D. K., S. R. Rose e J. C. Ronan. "Changing the Culture Around Cultures". Hospital Pediatrics 4, n.º 6 (1 de novembro de 2014): 405–7. http://dx.doi.org/10.1542/hpeds.2014-0064.

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Ortega Villasenor, Humberto, e Genaro Quinones Trujillo. "Aboriginal Cultures and Technocratic Culture". Essays in Philosophy 6, n.º 1 (2005): 226–34. http://dx.doi.org/10.5840/eip20056128.

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Threatened aboriginal cultures provide valuable criteria for fruitful criticism of the dominant Western cultural paradigm and perceptual model, which many take for granted as the inevitable path for humankind to follow. However, this Western model has proven itself to be imprecise and limiting. It obscures fundamental aspects of human nature, such as the mythical, religious dimension, and communication with the Cosmos. Modern technology, high-speed communication and mass media affect our ability to perceive reality and respond to it. Non-Western worldviews could help us to regain meaningful communication with Nature and to learn new ways of perceiving our world.

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Taufiqurrohman, Taufiqurrohman, Setyo Utomo e Purwo Adi Wibowo. "Cultured Trash, Not Trash Culture". E-DIMAS 8, n.º 2 (14 de setembro de 2017): 218. http://dx.doi.org/10.26877/e-dimas.v8i2.1360.

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As civilized creature, human actually can manage trash as well as possible although it is often stereotyped as a vain thing. This article gives the proof that trash can be cultured as well so that a society can take benefits from the existence of it. This article parses ways of orderly managing it at schools, in this case two schools in Jepara. The results say that trash can be cultured by having an organization to manage the Trash Bank at schools and to train students to classify and recycle trash then take advantage of it by selling the collected and the recycled trash. It makes trash have good transformation of values, repelling against the prior stereotype. Finally, by taking example from Trash Bank management at schools, human can have so cultured trash that they would not be trapped by trash culture.

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McKenzie, Stacey W., e Robert T. Means. "Blood Cultures and Culture Change". American Journal of the Medical Sciences 358, n.º 3 (setembro de 2019): 171–72. http://dx.doi.org/10.1016/j.amjms.2019.05.015.

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Artal-Tur, Andres. "Culture and cultures in tourism". Anatolia 29, n.º 2 (19 de dezembro de 2017): 179–82. http://dx.doi.org/10.1080/13032917.2017.1414433.

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Shafiq, Nusrat. "Antimicrobial Stewardship: ‘Culture of Cultures'". Journal of Antimicrobial Stewardship Practices and Infectious diseases 1, n.º 1 (31 de dezembro de 2023): 6–8. http://dx.doi.org/10.62541/jaspi016.

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Ylostalo, Joni H. "3D Stem Cell Culture". Cells 9, n.º 10 (27 de setembro de 2020): 2178. http://dx.doi.org/10.3390/cells9102178.

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Much interest has been directed towards stem cells, both in basic and translational research, to understand basic stem cell biology and to develop new therapies for many disorders. In general, stem cells can be cultured with relative ease, however, most common culture methods for stem cells employ 2D techniques using plastic. These cultures do not well represent the stem cell niches in the body, which are delicate microenvironments composed of not only stem cells, but also supporting stromal cells, extracellular matrix, and growth factors. Therefore, researchers and clinicians have been seeking optimal stem cell preparations for basic research and clinical applications, and these might be attainable through 3D culture of stem cells. The 3D cultures recapitulate the in vivo cell-to-cell and cell-to-matrix interactions more effectively, and the cells in 3D cultures exhibit many unique and desirable characteristics. The culture of stem cells in 3D may employ various matrices or scaffolds, in addition to the cells, to support the complex structures. The goal of this Special Issue is to bring together recent research on 3D cultures of various stem cells to increase the basic understanding of stem cells and culture techniques, and also highlight stem cell preparations for possible novel therapeutic applications.

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Contramaestre, A. P., F. Sifontes, R. Marín e M. I. Camejo. "Secretion of stem cell factor and granulocyte–macrophage colony-stimulating factor by mouse embryos in culture: influence of group culture". Zygote 16, n.º 4 (novembro de 2008): 297–301. http://dx.doi.org/10.1017/s0967199408004760.

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SummaryPrevious studies showed that the addition of a growth factor to the culture medium could modulate embryo development. The possible secretion of different factors to the culture medium by the embryo itself, however, has been poorly evaluated. The present study was designed to investigate: (1) the influence of single or group culture on the development of 2-cell mouse embryos (strain CD-1) to the blastocyst stage; (2) the release of granulocyte–macrophage colony-stimulating factor (GM-CSF) and stem cell factor (SCF) into the culture medium by the embryo; and (3) the levels of GM-CSF and SCF in the culture medium from both single and group embryos. Two-cell CD-1 mouse embryos were cultured for 96 h singly or in groups of five embryos per drop. GM-CSF and SCF were assayed by ELISA in the complete culture medium. It was found that embryos cultured in groups gave a higher percentage of total blastocyst formation and hatched blastocyst when compared with single embryo culture. The mouse embryos secreted GM-CSF and SCF to the culture medium. The concentration of these cytokines is significantly higher in the group cultures than the level found in single cultures. In conclusion, mouse embryos in culture secrete GM-CSF and SCF to the culture medium and the concentration of these cytokines increases during communal culture. These factors may be operating in both autocrine and paracrine pathways to modulate embryo development during in vitro culture.

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Wu, Shaofen. "Beyond Hofstede Dimension Model: A New Cultural Dimension of Context Culture". International Journal of Languages, Literature and Linguistics 9, n.º 1 (fevereiro de 2023): 90–93. http://dx.doi.org/10.18178/ijlll.2023.9.1.386.

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With significant political and economic changes over the decades, the limitations of Hofstede's cultural difference dimension model have been heated debated. The purpose of this research is to explore the levels of context culture among people in China of different ages and genders and whether context culture should be considered as part of the cultural dimension. The respondents were 385 people aged 20 to 40 in China, and the study was quantitative, using independent sample tests to answer research questions. This research shows that China is a high context culture country, and the women have higher context culture than men. In addition, context culture differs significantly in age groups, and the level of context culture increases with age. Therefore, context culture can be considered as a new cultural dimension, and it is also suggested to add this new dimension to Hofstede's cultural dimensions model to examine culture more comprehensively.

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First, NL, MM Sims, SP Park e MJ Kent-First. "Systems for production of calves from cultured bovine embryonic cells". Reproduction, Fertility and Development 6, n.º 5 (1994): 553. http://dx.doi.org/10.1071/rd9940553.

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The development of totipotent bovine embryonic cell cultures has great value in cattle breeding. They provide: (1) a mechanism for making large numbers of clonal offspring by nuclear transfer; (2) an efficient gene transfer system through the use of selectable markers to select transgenic cells; and (3) a mechanism for site-specific gene transfer or deletion by homologous DNA sequence recombination. Bovine embryonic cell cultures have been established from blastocyst inner cell mass (ICM) cells, morulae and the precompaction 16-20-cell stage. All have exhibited similar morphology to mouse embryonic stem (ES) cells, pluripotency on differentiation and proliferation in culture. Culture systems have consisted of microdrop loose suspension short-term cultures or long-term cultures on bovine or murine fibroblast feeder layers, in either a microdrop or a culture dish. The relative merit of culture systems or media requirements for mitosis and prevention of differentiation have not been determined. At present, totipotency is also unknown for cultured cells of the 16-20-cell stage. For cultured ICM cells, totipotency was demonstrated by the birth of four calves from ICM cells cultured 27 days or less in a loose suspension microdrop. Advanced pluripotency and perhaps totipotency was demonstrated in one fetus in a recently reported study where morulae cells cultured in vitro were chimaerized with non-cultured cells. DNA fingerprinting to associate cell lines with offspring and karyotyping to ascertain chromatin normalcy is important in ES cell research. Data pertaining to the use of each are presented.

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Santana López, Alejandra. "CULTURA JUVENIL Y CULTURA ESCOLAR. APROXIMACIÓN A UNA RELACIÓN COMPLEJA: TENSIONES Y DESAFÍOS". Foro Educacional, n.º 17 (20 de maio de 2015): 15. http://dx.doi.org/10.29344/07180772.17.641.

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RESUMEN:El presente artículo está enfocado en analizar la relación entre cultura juvenil y cultura escolar desde el contexto escolar, para esto se desarrolla una revisión bibliográfica preliminar, que permite comprender las lógicas de ambas culturas. Para terminar identificando tensiones y desafíos que esta relación plantea especialmente para quienes se desempeñan profesionalmente en contextos escolares.Se finaliza con un conjunto de conclusiones, en que se pretende sugerir algunas estrategias de acción con los jóvenes – escolares.Palabras clave: cultura juvenil, cultura escolar, escuela, intervenciones socioeducativas.YOUTH CULTURE AND SCHOOL CULTURE. ANAPPROACH TO A COMPLEX RELATIONSHIP: TENSIONSAND CHALLENGESABSTRACT:This article focuses on analyzing the relationship between youth culture and school culture from the school context, for it develops a preliminary literature review, which allows us to understand the logic of both cultures. Finally identifying tensions and challenges this relationship poses especially for those working professionally in school settings.It concludes with a set of conclusions, which are intended to suggest some strategies for action with young people - school.Keywords: youth culture, school culture, school, social and educational interventions.

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Lee, Yoonjung, Ahrang Lee, Hae Seong Jeong, Sung Un Shin, Uh Jin Kim, Seong Eun Kim, Seung-Ji Kang et al. "The microbiology of periprosthetic joint infections as revealed by sonicate cultures in Korea: Routine use of fungal and mycobacterial cultures is necessary?" PLOS ONE 19, n.º 8 (15 de agosto de 2024): e0309046. http://dx.doi.org/10.1371/journal.pone.0309046.

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Background Although sonication is a valuable diagnostic tool for periprosthetic joint infections (PJI), it is not commonly utilized. We analyzed sonicate and intraoperative tissue culture results obtained from three hospitals to define the microbial etiology of PJIs in Korea. Furthermore, we investigated necessity of conducting regular fungal and mycobacterial cultures. Methods We retrospectively analyzed data for patients with suspected orthopedic-related infections between 2017 and 2022, who had undergone prostheses removal surgery. We included 193 patients with suspected PJIs, and bacterial (n = 193), fungal (n = 193), and mycobacterial (n = 186) cultures were conducted on both sonicate and intraoperative tissue samples. The diagnosis of PJI was based on the European Bone and Joint Infection Society (EBJIS) criteria. Results Out of 193 patients, 121 (62.7%) had positive sonicate cultures, while 112 (58.0%) had positive periprosthetic tissue cultures. According to EBJIS criteria, a total of 181 patients were diagnosed with PJI, and 141 patients received microbiological confirmation through sonicate fluid culture or tissue culture. Of the 181 patients, 28 were classified with acute PJI (within 3 months of implantation) and 153 with chronic PJI. Among 141 patients, staphylococci were the most common organisms, accounting for 51.8% of cases, followed by Gram-negative organisms (15.6%), fungus (8.5%), and mycobacteria (3.5%). Nearly 91.7% of fungal isolates were Candida species, which also grew in bacterial cultures. In total, 11 cases cultured positive only in tissue culture, whereas 20 cases cultured positive only in sonicate culture. The antibiotic treatment plans were adjusted according to culture results. Conclusions Utilizing sonicate culture has greatly assisted in identifying pathogens responsible for chronic indolent PJIs, allowing suitable antimicrobial treatment. Based on few cases involving non-Candida and mycobacterial infections, it appears that routine fungal and mycobacterial cultures may not be necessary.

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Abad Merchan, Andres, e Fernando Lopez Parra. "La cultura organizacional versus la organización como cultura / Organizational culture versus the organization as culture". Ciencia Unemi 8, n.º 14 (24 de agosto de 2015): 113. http://dx.doi.org/10.29076/issn.2528-7737vol8iss14.2015pp113-121p.

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Este artículo aborda la comprensión de los diversos discursos que se han elaborado sobre la cultura organizacional tantoen su visión positivista concebida como variable, cuanto en su visión semiótica entendida como metáfora raíz. Se distinguenestas dos miradas para contraponer el paradigma funcionalista con el simbólico-interpretativo de la teoría organizacional.Por otra parte se hace mención de los modelos sobre cultura organizacional de Hatch y Schein, que han sido demayor divulgación. AbstractThis article discusses the understanding of various speeches which have been developed about organizational culture both froma positivist outlook in which it is conceived as a variable, as well as in its semiotic vision in which it is understood as a metaphor.These two perspectives are distinguished to contrast the functionalist with the symbolic-interpretative paradigm of organizationaltheory. On the other hand mention is made of the models on organizational culture of Hatch and Schein, who have beensubject to widespread analysis.

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Gassl, Vincent, Merel R. Aberle, Bas Boonen, Rianne D. W. Vaes, Steven W. M. Olde Damink e Sander S. Rensen. "Chemosensitivity of 3D Pancreatic Cancer Organoids Is Not Affected by Transformation to 2D Culture or Switch to Physiological Culture Medium". Cancers 14, n.º 22 (16 de novembro de 2022): 5617. http://dx.doi.org/10.3390/cancers14225617.

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Organoids are increasingly used to investigate patient-specific drug responsiveness, but organoid culture is complex and expensive, and carried out in rich, non-physiological media. We investigated reproducibility of drug-responsiveness of primary cell cultures in 2D versus 3D and in conventional versus physiological cell culture medium. 3D pancreatic ductal adenocarcinoma organoid cultures PANCO09b and PANCO11b were converted to primary cell cultures growing in 2D. Transformed 2D cultures were grown in physiological Plasmax medium or Advanced-DMEM/F12. Sensitivity towards gemcitabine, paclitaxel, SN-38, 5-fluorouacil, and oxaliplatin was investigated by cell viability assays. Growth rates of corresponding 2D and 3D cultures were comparable. PANCO09b had a shorter doubling time in physiological media. Chemosensitivity of PANCO09b and PANCO11b grown in 2D or 3D was similar, except for SN-38, to which PANCO11b cultured in 3D was more sensitive (2D: 8.2 ×10−3 ± 2.3 ×10−3 vs. 3D: 1.1 ×10−3 ± 0.6 ×10−3, p = 0.027). PANCO09b and PANCO11b showed no major differences in chemosensitivity when cultured in physiological compared to conventional media, although PANCO11b was more sensitive to SN-38 in physiological media (9.8 × 10−3 ± 0.7 × 10−3 vs. 5.2 × 10−3 ± 1.8 × 10−3, p = 0.015). Collectively, these data indicate that the chemosensitivity of organoids is not affected by culture medium composition or culture dimensions. This implies that organoid-based drug screens can be simplified to become more cost-effective.

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Son, Tae Gen, Yoojin Seo, Won-Tae Kim, Meesun Kim, Seon Jeong Choi, Si Ho Choi, Byung-Jun Sung, Jae-Seok Min, Eon Chul Han e Hyung-Sik Kim. "Characterization of 3D Organotypic Culture of Mouse Adipose-Derived Stem Cells". International Journal of Molecular Sciences 25, n.º 7 (1 de abril de 2024): 3931. http://dx.doi.org/10.3390/ijms25073931.

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Although stem cells are a promising avenue for harnessing the potential of adipose tissue, conventional two-dimensional (2D) culture methods have limitations. This study explored the use of three-dimensional (3D) cultures to preserve the regenerative potential of adipose-derived stem cells (ADSCs) and investigated their cellular properties. Flow cytometric analysis revealed significant variations in surface marker expressions between the two culture conditions. While 2D cultures showed robust surface marker expressions, 3D cultures exhibited reduced levels of CD44, CD90.2, and CD105. Adipogenic differentiation in 3D organotypic ADSCs faced challenges, with decreased organoid size and limited activation of adipogenesis-related genes. Key adipocyte markers, such as lipoprotein lipase (LPL) and adipoQ, were undetectable in 3D-cultured ADSCs, unlike positive controls in 2D-cultured mesenchymal stem cells (MSCs). Surprisingly, 3D-cultured ADSCs underwent mesenchymal–epithelial transition (MET), evidenced by increased E-cadherin and EpCAM expression and decreased mesenchymal markers. This study highlights successful ADSC organoid formation, notable MSC phenotype changes in 3D culture, adipogenic differentiation challenges, and a distinctive shift toward an epithelial-like state. These findings offer insights into the potential applications of 3D-cultured ADSCs in regenerative medicine, emphasizing the need for further exploration of underlying molecular mechanisms.

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Silva, Eliuvomar Cruz da, Laury Vander Leandro de Souza, Maria Francisca Nunes de Souza, Neli Brito Moreira, Nelly Mary Oliveira de Souza e Jennyfer de Souza Arancívia. "ESCOLA INTERCULTURAL". RCMOS - Revista Científica Multidisciplinar O Saber 3, n.º 1 (22 de janeiro de 2024): 1–5. http://dx.doi.org/10.51473/ed.al.v3i1.610.

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This article is an excerpt from the theoretical contribution that underlies the Doctoral Thesis that investigates – NATIONAL PROGRAM FOR TRAINING BASIC EDUCATION TEACHERS (PARFOR), IN THE MUNICIPALITY OF SANTO ANTÔNIO DO IÇA – AMAZONAS. The study shows that approaching the principle of interculturality implies an understanding of culture and its diff erent conceptions. We are a multi-ethnic and plural society and this implies a diversity of cultures. The reductionist conception ended up hierarchizing cultures by thinking of them in an evolutionary process, as if one culture had to evolve to reach the level of another. Thus, for a long time it was understood that there was a superior culture and other inferior cultures that should have come to it. In this case, the culture understood as cultured was the culture of Western Europe and its expressions took place through art, music, dance, its language, its religion.

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Lousã, Eva Petiz, e A. Duarte Gomes. "Evaluation of organizational culture: adaptation and validation of the portuguese version of the Denison Organizational Culture Survey". Psychologica 2, n.º 59 (2016): 101–21. http://dx.doi.org/10.14195/1647-8606_59_2_6.

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Jarrahy, Reza, Weibiao Huang, George H. Rudkin, Jane M. Lee, Kenji Ishida, Micah D. Berry, Modar Sukkarieh, Benjamin M. Wu, Dean T. Yamaguchi e Timothy A. Miller. "Osteogenic differentiation is inhibited and angiogenic expression is enhanced in MC3T3-E1 cells cultured on three-dimensional scaffolds". American Journal of Physiology-Cell Physiology 289, n.º 2 (agosto de 2005): C408—C414. http://dx.doi.org/10.1152/ajpcell.00196.2004.

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Osteogenic differentiation of osteoprogenitor cells in three-dimensional (3D) in vitro culture remains poorly understood. Using quantitative real-time RT-PCR techniques, we examined mRNA expression of alkaline phosphatase, osteocalcin, and vascular endothelial growth factor (VEGF) in murine preosteoblastic MC3T3-E1 cells cultured for 48 h and 14 days on conventional two-dimensional (2D) poly(l-lactide-co-glycolide) (PLGA) films and 3D PLGA scaffolds. Differences in VEGF secretion and function between 2D and 3D culture systems were examined using Western blots and an in vitro Matrigel-based angiogenesis assay. Expression of both alkaline phosphatase and osteocalcin in cells cultured on 3D scaffolds was significantly downregulated relative to 2D controls in 48 h and 14 day cultures. In contrast, elevated levels of VEGF expression in 3D culture were noted at every time point in short- and long-term culture. VEGF protein secretion in 3D cultures was triple the amount of secretion observed in 2D controls. Conditioned medium from 3D cultures induced an enhanced level of angiogenic activity, as evidenced by increases in branch points observed in in vitro angiogenesis assays. These results collectively indicate that MC3T3-E1 cells commit to osteogenic differentiation at a slower rate when cultured on 3D PLGA scaffolds and that VEGF is preferentially expressed by these cells when they are cultured in three dimensions.

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Sansupa, Chakriya, Sara Fareed Mohamed Wahdan, Terd Disayathanoowat e Witoon Purahong. "Identifying Hidden Viable Bacterial Taxa in Tropical Forest Soils Using Amplicon Sequencing of Enrichment Cultures". Biology 10, n.º 7 (22 de junho de 2021): 569. http://dx.doi.org/10.3390/biology10070569.

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This study aims to estimate the proportion and diversity of soil bacteria derived from eDNA-based and culture-based methods. Specifically, we used Illumina Miseq to sequence and characterize the bacterial communities from (i) DNA extracted directly from forest soil and (ii) DNA extracted from a mixture of bacterial colonies obtained by enrichment cultures on agar plates of the same forest soil samples. The amplicon sequencing of enrichment cultures allowed us to rapidly screen a culturable community in an environmental sample. In comparison with an eDNA community (based on a 97% sequence similarity threshold), the fact that enrichment cultures could capture both rare and abundant bacterial taxa in forest soil samples was demonstrated. Enrichment culture and eDNA communities shared 2% of OTUs detected in total community, whereas 88% of enrichment cultures community (15% of total community) could not be detected by eDNA. The enrichment culture-based methods observed 17% of the bacteria in total community. FAPROTAX functional prediction showed that the rare and unique taxa, which were detected with the enrichment cultures, have potential to perform important functions in soil systems. We suggest that enrichment culture-based amplicon sequencing could be a beneficial approach to evaluate a cultured bacterial community. Combining this approach together with the eDNA method could provide more comprehensive information of a bacterial community. We expected that more unique cultured taxa could be detected if further studies used both selective and non-selective culture media to enrich bacteria at the first step.

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Finoli, Anthony, Eva Schmelzer, Patrick Over, Ian Nettleship e Joerg C. Gerlach. "Open-Porous Hydroxyapatite Scaffolds for Three-Dimensional Culture of Human Adult Liver Cells". BioMed Research International 2016 (2016): 1–7. http://dx.doi.org/10.1155/2016/6040146.

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Liver cell culture within three-dimensional structures provides an improved culture system for various applications in basic research, pharmacological screening, and implantable or extracorporeal liver support. Biodegradable calcium-based scaffolds in such systems could enhance liver cell functionality by providing endothelial and hepatic cell support through locally elevated calcium levels, increased surface area for cell attachment, and allowing three-dimensional tissue restructuring. Open-porous hydroxyapatite scaffolds were fabricated and seeded with primary adult human liver cells, which were embedded within or without gels of extracellular matrix protein collagen-1 or hyaluronan. Metabolic functions were assessed after 5, 15, and 28 days. Longer-term cultures exhibited highest cell numbers and liver specific gene expression when cultured on hydroxyapatite scaffolds in collagen-1. Endothelial gene expression was induced in cells cultured on scaffolds without extracellular matrix proteins. Hydroxyapatite induced gene expression for cytokeratin-19 when cells were cultured in collagen-1 gel while culture in hyaluronan increased cytokeratin-19 gene expression independent of the use of scaffold in long-term culture. The implementation of hydroxyapatite composites with extracellular matrices affected liver cell cultures and cell differentiation depending on the type of matrix protein and the presence of a scaffold. The hydroxyapatite scaffolds enable scale-up of hepatic three-dimensional culture models for regenerative medicine applications.

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Kreß, Sebastian, Roland Schaller-Ammann, Jürgen Feiel, Joachim Wegener, Joachim Priedl, Wolf Dietrich, Cornelia Kasper e Dominik Egger. "Innovative Platform for the Advanced Online Monitoring of Three-Dimensional Cells and Tissue Cultures". Cells 11, n.º 3 (25 de janeiro de 2022): 412. http://dx.doi.org/10.3390/cells11030412.

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The use of 3D cell cultures has gained increasing importance in medical and pharmaceutical research. However, the analysis of the culture medium is hardly representative for the culture conditions within a 3D model which hinders the standardization of 3D cultures and translation of results. Therefore, we developed a modular monitoring platform combining a perfusion bioreactor with an integrated minimally invasive sampling system and implemented sensors that enables the online monitoring of culture parameters and medium compounds within 3D cultures. As a proof-of-concept, primary cells as well as cell lines were cultured on a collagen or gelatin methacryloyl (GelMA) hydrogel matrix, while monitoring relevant culture parameters and analytes. Comparing the interstitial fluid of the 3D models versus the corresponding culture medium, we found considerable differences in the concentrations of several analytes. These results clearly demonstrate that analyses of the culture medium only are not relevant for the development of standardized 3D culture processes. The presented bioreactor with an integrated sampling and sensor platform opens new horizons for the development, optimization, and standardization of 3D cultures. Furthermore, this technology holds the potential to reduce animal studies and improve the transferability of pharmaceutical in vitro studies by gaining more relevant results, bridging the gap towards clinical translation.

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Citot, Vincent. "L'idée d'une Europe de la Culture (L'Europe des cultures, la culture européenne et l'Europe de la Culture)". Le Philosophoire 27, n.º 2 (2006): 215. http://dx.doi.org/10.3917/phoir.027.0215.

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Stokes, Rebecca A., Michelle C. Coleman, Artem S. Rogovskyy, Vanna M. Dickerson e Kelley M. Thieman Mankin. "Comparison of bacteriologic culture results for skin wound swabs and skin wound biopsy specimens". Journal of the American Veterinary Medical Association 259, n.º 12 (15 de dezembro de 2021): 1416–21. http://dx.doi.org/10.2460/javma.20.10.0568.

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Abstract OBJECTIVE To compare bacteriologic culture results for superficial swab and tissue biopsy specimens obtained from dogs with open skin wounds. ANIMALS 52 client-owned dogs. PROCEDURES For each dog, 1 wound underwent routine preparation prior to collection of 2 specimens, 1 by superficial swab (Levine) technique and 1 by tissue biopsy. Specimens were processed for bacteriologic culture. Two observers determined whether any detected difference in culture results for the 2 types of specimen would have resulted in differing treatment plans. RESULTS Culture results of swab and tissue biopsy specimens were identical in 11/52 (21.2%) cases. Tissue biopsy specimen and swab cultures yielded positive results for 44 (84.6%) and 40 (76.9%) wounds, respectively. With regard to mean recovery rates of bacteria from wounds with positive culture results, both the biopsy specimens and swabs yielded 3.4 bacterial species/wound. All wounds for which swab cultures yielded no growth also had negative culture results for biopsy specimens. Biopsy specimen and swab culture results were in agreement with regard to the most common bacteria cultured. In 7/52 (13%) wounds, the observers would have treated the patient differently on the basis of the results of the 2 cultures. CONCLUSIONS AND CLINICAL RELEVANCE Results indicated that culture of a swab collected by the Levine technique is an appropriate noninvasive alternative to culture of a tissue biopsy specimen. A negative result obtained from culture of a swab is likely to be reliable. Disagreement between the results of swab and tissue biopsy specimen cultures is likely of low clinical importance.

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Huang, Xiaosong, L. Jeanne Pierce, Paul A. Cobine, Dennis R. Winge e Gerald J. Spangrude. "Copper Modulates the Differentiation of Mouse Hematopoietic Progenitor Cells in Culture". Cell Transplantation 18, n.º 8 (agosto de 2009): 887–97. http://dx.doi.org/10.3727/096368909x471152.

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Copper chelation has been shown to favor the expansion of human hematopoietic stem/progenitor cells in vitro. To further understand the effects of copper modulation on defined subsets of stem cells versus progenitor cells, we extended the studies in a mouse system. We isolated mouse hematopoietic stem cells (HSCs) or hematopoietic progenitor cells (HPCs) and cultured them with or without the copper chelator tetraethylenepentamine (TEPA) or CuCl2. Cytokine-stimulated HPC cultures treated with TEPA for 7 days generated about two to three times more total and erythroid colony-forming cells (CFCs) compared to control cultures. In contrast, CuCl2 treatment decreased the CFC numbers. Similar results were seen with HSC after 14, but not 7, days of culture. Transplant studies showed that HPCs cultured for 7 days in TEPA had about twofold higher short-term erythroid repopulation potential compared to control cultures, while CuCl2 decreased the erythroid potential of cultured HPCs compared to control cultures. HSCs cultured with TEPA for 7 days did not exhibit significantly higher repopulation potential in either leukocyte or erythrocyte lineages compared to control cultures in short-term or long-term assays. Based on JC-1 staining, the mitochondrial membrane potential of HPCs cultured with TEPA was lower relative to control cultures. Our data suggest that decreasing the cellular copper content with TEPA results in preferential expansion or maintenance of HPC that are biased for erythroid differentiation in vivo, but does not enhance the maintenance of HSC activity in culture.

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Persson, Per-Edvin. "Odorous Algal Cultures in Culture Collections". Water Science and Technology 20, n.º 8-9 (1 de agosto de 1988): 211–13. http://dx.doi.org/10.2166/wst.1988.0245.

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The IAWPRC Specialist Group on Tastes and Odours collected a list of odorous algal cultures available from culture collections around the world. A list of 42 algal strains is presented, supplemented by data on their odour, purity and where to obtain them.

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Friedman, Jonathan. "Culture et politique de la culture1". Anthropologie et Sociétés 28, n.º 1 (19 de novembro de 2004): 23–43. http://dx.doi.org/10.7202/008569ar.

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RésuméCet article traite de ce qui détermine les politiques de la culture. L’argument central est que les formes de culture résultent de l’expérience socialement construite – ce que j’appelle le substrat expérientiel de la culture – et que celui-ci voit le jour dans le contexte d’une existence sociale qui est elle-même reliée à des processus globaux. En abordant l’histoire du concept de culture dans ces termes, je montre que l’émergence des politiques identitaires depuis le milieu des années 1970 est en rapport avec ce processus plus large qu’est le déclin de l’hégémonie occidentale. De ce point de vue, les nouvelles politiques de la culture révèlent une véritable fragmentation politique et culturelle. Mais ce déclin constitue la base de la globalisation politico-économique et grâce à lui émergent des élites cosmopolites qui deviennent les hérauts du discours de globalisation. Ce discours fait à son tour partie de la polarisation de classe qui voit s’affirmer les nouvelles élites cosmopolites « hybrides » alors que s’éclipsent les pouvoirs locaux indigènes.

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Feyerabend, Paul. "Potentially Every Culture Is All Cultures". Common Knowledge 25, n.º 1-3 (1 de abril de 2019): 13–20. http://dx.doi.org/10.1215/0961754x-7299054.

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With a focus on the role of Achilles in the Iliad, this essay argues that it is a mistake to assume that languages and cultures are closed totalities impervious to influence from the outside and from internally driven transformations. If we eliminate that assumption, several others likewise become untenable, including the assumption that precise meanings for words or concepts are available in principle. Objectivism and relativism are claimed to be equally chimerical.

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Hitchcock, Michael, e Michel Picard. "Bali: Tourisme culturel et culture touristique." Man 29, n.º 2 (junho de 1994): 496. http://dx.doi.org/10.2307/2804519.

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Franklin, Sarah. "Science as Culture, Cultures of Science". Annual Review of Anthropology 24, n.º 1 (outubro de 1995): 163–84. http://dx.doi.org/10.1146/annurev.an.24.100195.001115.

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Wall, Geoffrey. "Bali: Tourisme culturel et culture touristique". Annals of Tourism Research 21, n.º 2 (janeiro de 1994): 437–39. http://dx.doi.org/10.1016/0160-7383(94)90070-1.

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Chauveau, Gérard, e Eliane Rogovas-Chauveau. "L'école, la culture et les cultures". Migrants formation 102, n.º 1 (1995): 6–19. http://dx.doi.org/10.3406/diver.1995.7463.

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Halid, Ahmad. "Tujuh Budaya Tujuh Budaya Masyarakat Pegunungan: Reposisi Pesantren Sebagai Pembimbing Masyarakat". Al-Mada: Jurnal Agama, Sosial, dan Budaya 4, n.º 1 (6 de maio de 2021): 138–56. http://dx.doi.org/10.31538/almada.v4i1.790.

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Culture is beliefs, norms, values, assumptions held by the community. The research method was determined by using a qualitative approach and collecting observation, interview, and documentation data. Data analysis using the Miles and Huberman model. A developed society is a society that is highly cultured and maintains its culture. Mountain culture is the culture of Amaen paraben (matchmaking), culture (aghelluk) pencak (ocular strength and kanoragan science), Magic / Guna-use culture, Toktok culture (united cattle complaints), ngalak chukok kalaot culture (mutual cooperation to catch fish) in the context of marriage or kalamam children greeting khatam al-qur'an, salvation three, seven, forty, one hundred days of death of a person or other celebration), salamatan janur katopak (village salamatan) culture and kalongan katopak and jhubethe cattle culture. These seven cultures have been selected and colored by pesantren so that it becomes a culture that is beneficial to people's lives.

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Culp, D. J., e L. R. Latchney. "Mucinlike glycoproteins from cat tracheal gland cells in primary culture". American Journal of Physiology-Lung Cellular and Molecular Physiology 265, n.º 3 (1 de setembro de 1993): L260—L269. http://dx.doi.org/10.1152/ajplung.1993.265.3.l260.

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In a recent study (D. J. Culp, D. K. P. Lee, D. P. Penney, and M. G. Marin. Am. J. Physiol. 263: L264-275, 1992), we reported that primary cultures of cat tracheal gland cells expressed histological, ultrastructural, and immunological characteristics of mucous cells when cultured on floating gels of rat tail collagen (released-gel cultures) compared with cells cultured on glutaraldehyde-fixed collagen gels (fixed-gel cultures). We therefore collected culture medium from gland cells grown under both culture conditions for determination and comparison of glycoconjugates with characteristics of mucin glycoproteins. Cells were cultured in the presence of [3H]glucosamine, and material of high molecular weight and density (HMD material) was isolated. HMD material from both culture conditions were each resistant to heparitinase and heparinase, whereas 72 and 25% of the radiolabel in released-gel and fixed-gel HMD material, respectively, was resistant to chondroitinase ABC. Material resistant to chondroitinase ABC was analyzed further. Both samples contained a single broad glycoprotein band [relative molecular weight (M(r)) > 250,000] after sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and had amino acid profiles similar to airway mucin. The sample from fixed-gel cultures had nearly equal amounts of carbohydrate and protein, was highly enriched in N-acetylglucosamine, contained mannose, displayed little blood group A immunoreactivity, and had few O-linked oligosaccharides. Conversely, the sample from released-gel cultures contained 80% carbohydrate, was composed of monosaccharides characteristic of airway mucins, displayed blood group A immunoreactivity, and contained oligosaccharides O-linked via N-acetylgalactosamine.(ABSTRACT TRUNCATED AT 250 WORDS)

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