Bibliografias temáticas / Charge Transfert Inefficiency

Índice

Artigos de revistas
Teses / dissertações
Capítulos de livros
Trabalhos de conferências
Relatórios de organizações

Literatura científica selecionada sobre o tema "Charge Transfert Inefficiency"

Autor: Grafiati

Publicado: 25 de janeiro de 2025

Crie uma referência precisa em APA, MLA, Chicago, Harvard, e outros estilos

Selecione um tipo de fonte:

Consulte a lista de atuais artigos, livros, teses, anais de congressos e outras fontes científicas relevantes para o tema "Charge Transfert Inefficiency".

Ao lado de cada fonte na lista de referências, há um botão "Adicionar à bibliografia". Clique e geraremos automaticamente a citação bibliográfica do trabalho escolhido no estilo de citação de que você precisa: APA, MLA, Harvard, Chicago, Vancouver, etc.

Você também pode baixar o texto completo da publicação científica em formato .pdf e ler o resumo do trabalho online se estiver presente nos metadados.

Artigos de revistas sobre o assunto "Charge Transfert Inefficiency"

Pasquier, J. F. "Native and irradiated Charge Transfer Inefficiency characterization". EAS Publications Series 45 (2010): 61–66. http://dx.doi.org/10.1051/eas/1045010.

Texto completo da fonte

Estilos ABNT, Harvard, Vancouver, APA, etc.

Nightingale, James W., Richard J. Massey, Jacob Kegerreis e Richard G. Hayes. "PyAutoCTI: Open-Source Charge Transfer Inefficiency Calibration". Journal of Open Source Software 9, n.º 98 (1 de junho de 2024): 4904. http://dx.doi.org/10.21105/joss.04904.

Texto completo da fonte

Estilos ABNT, Harvard, Vancouver, APA, etc.

Stetson, Peter B. "On the Photometric Consequences of Charge‐Transfer Inefficiency in WFPC2". Publications of the Astronomical Society of the Pacific 110, n.º 754 (dezembro de 1998): 1448–63. http://dx.doi.org/10.1086/316286.

Texto completo da fonte

Estilos ABNT, Harvard, Vancouver, APA, etc.

Maneuski, Dzmitry. "Simulation of the charge transfer inefficiency of column parallel CCDs". Nuclear Instruments and Methods in Physics Research Section A: Accelerators, Spectrometers, Detectors and Associated Equipment 591, n.º 1 (junho de 2008): 252–54. http://dx.doi.org/10.1016/j.nima.2008.03.066.

Texto completo da fonte

Estilos ABNT, Harvard, Vancouver, APA, etc.

Bouchy, F., J. Isambert, C. Lovis, I. Boisse, P. Figueira, G. Hébrard e F. Pepe. "Charge Transfer Inefficiency effect for high-precision radial velocity measurements". EAS Publications Series 37 (2009): 247–53. http://dx.doi.org/10.1051/eas/0937031.

Texto completo da fonte

Estilos ABNT, Harvard, Vancouver, APA, etc.

Smith, P. H., J. P. D. Gow, P. Pool e A. D. Holland. "Charge transfer inefficiency in the pre- and post-irradiated Swept Charge Device CCD236". Journal of Instrumentation 10, n.º 03 (24 de março de 2015): C03041. http://dx.doi.org/10.1088/1748-0221/10/03/c03041.

Texto completo da fonte

Estilos ABNT, Harvard, Vancouver, APA, etc.

Blake, C. H., S. Halverson e A. Roy. "The impact of charge transfer inefficiency on Extreme Precision Doppler measurements". Journal of Instrumentation 12, n.º 04 (3 de abril de 2017): C04003. http://dx.doi.org/10.1088/1748-0221/12/04/c04003.

Texto completo da fonte

Estilos ABNT, Harvard, Vancouver, APA, etc.

Rhodes, Jason, Alexie Leauthaud, Chris Stoughton, Richard Massey, Kyle Dawson, William Kolbe e Natalie Roe. "The Effects of Charge Transfer Inefficiency (CTI) on Galaxy Shape Measurements". Publications of the Astronomical Society of the Pacific 122, n.º 890 (abril de 2010): 439–50. http://dx.doi.org/10.1086/651675.

Texto completo da fonte

Estilos ABNT, Harvard, Vancouver, APA, etc.

Townsley, L. K., P. S. Broos, J. A. Nousek e G. P. Garmire. "Modeling charge transfer inefficiency in the Chandra Advanced CCD Imaging Spectrometer". Nuclear Instruments and Methods in Physics Research Section A: Accelerators, Spectrometers, Detectors and Associated Equipment 486, n.º 3 (julho de 2002): 751–84. http://dx.doi.org/10.1016/s0168-9002(01)02156-8.

Texto completo da fonte

Estilos ABNT, Harvard, Vancouver, APA, etc.

Toyozumi, Hiroyuki, e Michael C. B. Ashley. "Intra-Pixel Sensitivity Variation and Charge Transfer Inefficiency — Results of CCD Scans". Publications of the Astronomical Society of Australia 22, n.º 3 (2005): 257–66. http://dx.doi.org/10.1071/as05013.

Texto completo da fonte

Resumo:

AbstractThe efficiency with which a charge-coupled device (CCD) detects photons depends, amongst other factors, on where within a pixel the photon hits. To explore this effect we have made detailed scans across a pixel for a front-illuminated three-phase EEV05-20 CCD using the standard astronomical B, V, R, and I colour filters. Pixel response functions and photometric sensitivity maps are derived from the scan images. Nonlinear charge transfer inefficiency (CTI) effects were observed and corrected for. The resulting images clearly show the intra-pixel sensitivity variations (IPSVs) due to the CCD electrode structure, and its dependence on wavelength. We briefly comment on the implications of IPSVs and CTI for high-precision photometry and astrometry.

Estilos ABNT, Harvard, Vancouver, APA, etc.

Mais fontes

Teses / dissertações sobre o assunto "Charge Transfert Inefficiency"

Salih, Alj Antoine. "Effets des radiations et propriétés électriques d’un capteur CCD-sur-CMOS à tranchées profondes actives pour l’imagerie haute-performance". Electronic Thesis or Diss., Toulouse, ISAE, 2024. http://www.theses.fr/2024ESAE0048.

Texto completo da fonte

Resumo:

Les dispositifs d'imagerie CMOS (Complementary Metal Oxide Semiconductor), trouvent de nombreuses applications en imagerie terrestre haute résolution et en imagerie scientifique (par exemple : Sentinel-2, MSL2020 et MMX). Les avancées impressionnantes réalisées ces cinq dernières années en technologie d'imagerie CMOS, tant au niveau des performances de photodétection que de la réduction du bruit, ouvrent aujourd'hui la voie à des applications de très haute performance, alors que les CCD (Charge Coupled Device) étaient jusqu'à présent les meilleurs candidats.Pour de telles applications, le développement de cette technologie doit se concentrer sur l'amélioration du rapport signal-à-bruit (SNR) afin d'atteindre une résolution spatiale optimale pour les images satellites d'observation terrestre (résolution sub-métrique). Le premier levier d'amélioration repose sur l'augmentation de la sensibilité du détecteur, avec comme objectif l'optimisation du transfert de charge inter-pixels et la réduction des courants d'obscurité parasites. Le second levier consiste à maximiser la capacité de collection de charge et à contrôler les effets de saturation. L'ensemble de ces paramètres doit être évalué en tenant compte des conditions de l'environnement spatial, en particulier les effets des radiations (ionisation et déplacement), qui peuvent fortement altérer les propriétés électriques des capteurs d'images.La technologie CMOS actuellement privilégiée pour les futurs projets d'imagerie terrestre haute résolution intègre un nœud technologique spécifique de tranchée profonde active. Cette technologie permet, lorsqu'elle est associée au potentiel de tranchée adapté, de contrôler les mouvements de charge dans le silicium. Ainsi, des registres à transfert de charge de type CCD-sur-CMOS utilisant cette technologie, ont été implémentés avec succès. L'analyse théorique et la caractérisation de certaines architectures de registres CCD à deux phases ont donné des résultats très prometteurs et ouvert de nouvelles perspectives.Les objectifs de cette thèse sont multiples : améliorer la compréhension de ce nouveau type de pixel à transfert de charge, et en particulier des tranchées profondes actives, à travers une analyse approfondie des phénomènes physiques en jeu et des effets des radiations (tant en termes de dose d'ionisation que de déplacement). Il s'agit également d'évaluer et de proposer des optimisations de conception adaptées à plusieurs modes de fonctionnement (Time Delay Integration, Electron Multiplication), permettant d'atteindre les performances visées en matière de SNR, tout en répondant aux exigences de tolérance aux radiations pour l'imagerie très haute résolution
CMOS imaging devices (Complementary Metal Oxide Semiconductor) have numerous applications in high-resolution terrestrial imaging and scientific imaging (e.g., Sentinel-2, MSL2020, and MMX). The remarkable advancements made in CMOS imaging technology over the past five years, both in terms of photodetection performance and noise reduction, have paved the way for very high-performance applications, where CCDs (Charge Coupled Devices) were previously considered the best candidates.For such applications, the development of this technology must focus on improving the signal-to-noise ratio (SNR) to achieve optimal spatial resolution in satellite images for terrestrial observation (sub-meter resolution). The first lever for improvement is increasing detector sensitivity, to optimize inter-pixel charge transfer and reduce parasitic dark currents. The second lever is maximizing charge collection capacity and controlling saturation effects. All these parameters must be evaluated considering the space environment, particularly the effects of radiation (ionization and displacement), which can significantly degrade the electrical properties of image sensors.The CMOS technology currently favored for future high-resolution terrestrial imaging projects integrates a specific feature of active deep trench isolation. When combined with the appropriate trench potential, this technology allows the control of charge movements within the silicon. As a result, CCD-on-CMOS charge transfer registers using this technology have been successfully implemented. Theoretical analysis and characterization of certain two-phase CCD register architectures have yielded very promising results and opened up new perspectives.The objectives of this thesis are multiple: to improve the understanding of this new type of charge transfer pixel, particularly the active deep trench isolation feature, through an in-depth analysis of the physical phenomena involved and the effects of radiation (both in terms of ionizing dose and displacement). Additionally, it aims to evaluate and propose design optimizations for various operating modes (Time Delay Integration, Electron Multiplication), to achieve the targeted SNR performance while meeting radiation tolerance requirements for high-resolution imaging

Estilos ABNT, Harvard, Vancouver, APA, etc.

Capítulos de livros sobre o assunto "Charge Transfert Inefficiency"

Pestieau, Pierre. "Efficiency of the welfare state". In The Welfare State in the European Union, 78–90. Oxford University PressOxford, 2005. http://dx.doi.org/10.1093/oso/9780199261017.003.0008.

Texto completo da fonte

Resumo:

Abstract In general, when one considers the concept of efficiency coupled with the welfare state, one immediately thinks of the effects on the efficiency of the economy of the welfare state, notably the taxes implied and the benefits generated. This question has been widely discussed in recent works.É One of the main charges addressed to modern welfare states is that they impair economic performance and international competitiveness. It was the topic of Chapter 7. Another charge, just as widespread, is their inefficiency in providing social services, and their responsibility for the proliferation of costly transfer programs that miss their target populations. This second charge is thus different from the first one, although not totally unrelated. It concerns the economic efficiency of the welfare state per se, and it is the topic of this chapter.

Estilos ABNT, Harvard, Vancouver, APA, etc.

Colonnelli, Emanuele, e Nicole Ntungire. "Construction and Public Procurement in Uganda". In Mining for Change, 326–48. Oxford University Press, 2020. http://dx.doi.org/10.1093/oso/9780198851172.003.0015.

Texto completo da fonte

Resumo:

As it transitions to an oil-producing country, Uganda’s investments in infrastructure and physical capital will increasingly depend on the ability of the construction sector to respond to surges in demand and transform investment effort into outcomes. Using administrative and survey data, this chapter sets out to examine the current bottlenecks to production faced by the construction sector in Uganda and identifies possible policy remedies to relieve them. A secondary point of emphasis in the chapter’s analysis is the interaction between government and construction firms through public procurement, and the instrumental role procurement plays in the efficient development of the industry. These new opportunities and challenges raise several questions. How much of the increased economic activity will be passed to the domestic private sector? Will these opportunities motivate efficiency and transparency? Would corruption and inefficiency in public procurement still play a major role as a barrier to industry development?

Estilos ABNT, Harvard, Vancouver, APA, etc.

Alabbasi, Yousef, e Kamaljeet Sandhu. "Blockchain Innovation and Information Technology at GCC". In Research Anthology on Blockchain Technology in Business, Healthcare, Education, and Government, 751–64. IGI Global, 2021. http://dx.doi.org/10.4018/978-1-7998-5351-0.ch044.

Texto completo da fonte

Resumo:

Blockchain has become an epidemic and significant decision that organizations may make in the next few years, enabling institutions to integrate business functions, operations, and processes in a decentralized distributed ledger technology. This technology will transform the business world and economy in solving the limitations created by centralization and system inefficiency. Accordingly, with the high demand and complexity of growing economies such as the Gulf Cooperation Council GCC countries, the need for a typical solution technology is a game changer. This will lead GCC to a solid economic base. Blockchain technology can be applicable in many different fields such as Banking, education, Health, finance, government and trade. This article will address the literature review and methodology of Blockchain technology and innovation at the GCC, particularly in Saudi Arabia. Also, more research can be conducted in the future as the system may be integrated in these countries.

Estilos ABNT, Harvard, Vancouver, APA, etc.

Alabbasi, Yousef, e Kamaljeet Sandhu. "The Framework for Blockchain Innovation and the Impact on Digital Economic Transformation". In Research Anthology on Blockchain Technology in Business, Healthcare, Education, and Government, 172–84. IGI Global, 2021. http://dx.doi.org/10.4018/978-1-7998-5351-0.ch009.

Texto completo da fonte

Resumo:

Blockchain technology has become an epidemic and significant decision that organizations may make in the next few years, as integrated business solution enabling institutions to integrate business functions, operations, and processes in a decentralized distributed ledger technology. This technology will transform the business world and economy in solving the limitations created by centralization and system inefficiency. Accordingly, with the highly demanding and complexity of growing economies such as Gulf Cooperation Council GCC countries, the need for a typical solution technology is a game changer. The result of this will lead GCC to a solid base of the economy. Blockchain technology can be applicable in many different fields such as: banking, education, health care, finance, government, trade, etc. This article will propose a conceptual framework for the acceptance of Blockchain technology and innovation in the GCC, particularly in Saudi Arabia. Also, more research can be conducted in the future as the system might be integrated in these countries.

Estilos ABNT, Harvard, Vancouver, APA, etc.

Musungwini, Samuel, Yvonne Madongonda e Hope Hogo. "Contemporary Agriculture Marketing Strategies for Smallholder Farmers in a Developing Context". In Sustainable Practices for Agriculture and Marketing Convergence, 200–225. IGI Global, 2024. http://dx.doi.org/10.4018/979-8-3693-2011-2.ch009.

Texto completo da fonte

Resumo:

For millions of people in developing nations, the agriculture industry is essential to their livelihoods, economic growth, and food security. It does, however, confront several difficulties, such as constrained resource availability, inefficient markets, and susceptibility to environmental fluctuations. Within this framework, integrating emerging technology offers a strong chance to transform marketing for agriculture and promote sustainable growth. This chapter explores the revolutionary prospective of using cutting-edge technologies for sustainable agriculture marketing in underdeveloped nations using a case study in Zimbabwe. Through an analysis of the convergence of technology, agriculture, and marketing in the distinctive circumstances of developing nations, the goal is to furnish stakeholders with significant perspectives and useful recommendations for harnessing innovation for the productive transformation of smallholder agricultural marketing practices.

Estilos ABNT, Harvard, Vancouver, APA, etc.

Matos, Douglas Nuernberg de, e Samantha Zamberlan Leyraud. "Use of antimicrobial seal in central venous catheter in hemodialysis patients". In Health and Medicine: Science, Care, and Discoveries. Seven Editora, 2024. http://dx.doi.org/10.56238/sevened2023.004-037.

Texto completo da fonte

Resumo:

Antibiotic lock therapy is an adjunctive therapy used in the treatment of bloodstream infections related to central venous catheters for hemodialysis and consists of the use of high concentrations of ATB to close the lumens between hemodialysis sessions. The central venous catheter is the least desirable form of vascular access due to its 2- to 3-fold increased risk of morbidity and mortality. Procedures to maintain vascular access can result in inefficient treatments and recurrent hospital admissions, and are constantly a major problem for the healthcare system. Contamination of the external and internal surface of the central venous catheter by the extraluminal and intraluminal routes, respectively, involves the transfer of microorganisms during manipulation of the central venous catheter, as well as during dressing changes or central venous catheter connection and disconnection. The purpose of the antimicrobial seal is to prevent colonization and biofilm formation, for which an antimicrobial agent is required. An anticoagulant is needed to prevent catheter dysfunction. This intervention can also be an adjunct to systemic antibiotic therapy and consists of rescuing the catheter by instilling the solution into each lumen of the catheter at the end of each dialysis session for the same duration of systemic therapy, when there is an ongoing bloodstream infection (BSI).

Estilos ABNT, Harvard, Vancouver, APA, etc.

Hnylianska, Olha. "BENCHMARKING AS A NEW COMPONENT FOR EFFECTIVE DEVELOPMENT OF THE ENTERPRISE". In Scientific space in the conditions of global transformations of the modern world. Publishing House “Baltija Publishing”, 2022. http://dx.doi.org/10.30525/978-9934-26-255-5-5.

Texto completo da fonte

Resumo:

Global competition, incentives for quality, global achievements in the areas of production and business technologies, adaptation to rapidly changing conditions require the search for new effective approaches and ways of doing business. A modern management tool is benchmarking as an integral part of strategic planning and improvement of the company’s activities. The article examines the essence of benchmarking as an important component of improving business processes in order to increase the level of competitiveness of the enterprise. The essence is considered, the types of benchmarking that should be taken into account to ensure effective comparative analysis are determined, and its shortcomings that must be avoided are considered. It has been proven that the result of benchmarking is the achievement of commercial success, faster and optimal management decision-making. Peculiarities of the implementation of benchmarking are the use of the experience of leading companies in various industries; a detailed study of the competition from the point of interest of the company’s activities; development of a strategy focusing on key success factors. The purpose of the article is to identify the role of benchmarking in the development of the company’s potential, the implementation of which will increase the company’s competitiveness.The research methodology is based on benchmarking and conducting research in this area, which will allow the company to become competitive. This analysis has proven its effectiveness as an active process that allows you to transform inefficient production into successful and profitable production. Practical consequences of a comparative analysis of the company’s work with the best representatives of the industry (or other industries), which consists in evaluating one’s own and industry efficiency, identifying and studying the work of industry leaders, and implementing the best industry achievements in one’s activities. Value/originality. Benchmarking helps determine the company’s strengths and weaknesses depending on changes in the market situation, makes it possible to better satisfy consumer demands for quality, price, goods and services by establishing new standards and goals. Thanks to benchmarking, the quality of products increases, the productivity and efficiency of business processes increases, innovative ideas are formed that contribute to increasing the level of competitiveness of the enterprise.

Estilos ABNT, Harvard, Vancouver, APA, etc.

Trabalhos de conferências sobre o assunto "Charge Transfert Inefficiency"

Nyren, Daniel, Marc Tardiff e Kenneth Desabrais. "Passive Stabilization and Stability Quantification of Helicopter Sling Load Payloads". In Vertical Flight Society 71st Annual Forum & Technology Display, 1–11. The Vertical Flight Society, 2015. http://dx.doi.org/10.4050/f-0071-2015-10227.

Texto completo da fonte

Resumo:

Helicopter Sling Load, while the most accurate form of aerial delivery, can be the most costly and inefficient materiel delivery option due to payload instabilities. These instabilities cause a reduction in airspeed and, therefore, increase the risk of being exposed to enemy fire while in hostile areas, increase the time of delivery, reduce the effective mission range, and increase the mission cost. This paper describes the design, wind tunnel, and half-scale flight testing of several flexible (fabric) passive stability concepts, including rigging changes and materiel prototypes. The most successful device demonstrated was a fin attached between the aft two sling legs. This fin was simple to attach to the payload and could be compact enough to store in a pocket. A method of quantifying stability of tethered bluff bodies was also demonstrated as the integration of the Fast Fourier Transform in three primary load instability motions. This stability coefficient showed excellent correlation with qualitative data, and therefore could be used to rank effectiveness of stability devices.

Estilos ABNT, Harvard, Vancouver, APA, etc.

SOPCZAK, ANDRÉ. "LCFI CHARGE TRANSFER INEFFICIENCY STUDIES FOR CCD VERTEX DETECTORS". In Proceedings of the 9th Conference. WORLD SCIENTIFIC, 2006. http://dx.doi.org/10.1142/9789812773678_0140.

Texto completo da fonte

Estilos ABNT, Harvard, Vancouver, APA, etc.

Gow, Jason P. D., e Neil J. Murray. "Simplified charge transfer inefficiency correction in CCDs by trap-pumping". In SPIE Astronomical Telescopes + Instrumentation, editado por Andrew D. Holland e James Beletic. SPIE, 2016. http://dx.doi.org/10.1117/12.2232706.

Texto completo da fonte

Estilos ABNT, Harvard, Vancouver, APA, etc.

Kelman, Bradley, Thibaut Prod'homme, Jesper Skottfelt, Frederic Lemmel, Matej Arko, Patricia Liebing, Peter Verhoeve, Ben Dryer, David Hall e Michael Hubbard. "Calibrating and correcting charge transfer inefficiency in CCDs using Pyxel". In X-Ray, Optical, and Infrared Detectors for Astronomy X, editado por Andrew D. Holland e James Beletic. SPIE, 2022. http://dx.doi.org/10.1117/12.2629896.

Texto completo da fonte

Estilos ABNT, Harvard, Vancouver, APA, etc.

Gow, J. P. D., e N. J. Murray. "Charge transfer inefficiency mitigation in a CCD by trap pumping". In 2016 16th European Conference on Radiation and its Effects on Components and Systems (RADECS). IEEE, 2016. http://dx.doi.org/10.1109/radecs.2016.8093128.

Texto completo da fonte

Estilos ABNT, Harvard, Vancouver, APA, etc.

Grant, C. E., M. W. Bautz, S. E. Kissel, B. LaMarr e G. Y. Prigozhin. "Temperature dependence of charge transfer inefficiency in Chandra X-ray CCDs". In SPIE Astronomical Telescopes + Instrumentation, editado por David A. Dorn e Andrew D. Holland. SPIE, 2006. http://dx.doi.org/10.1117/12.672019.

Texto completo da fonte

Estilos ABNT, Harvard, Vancouver, APA, etc.

Ahmed, Saad, David J. Hall, Cian Crowley, Jesper M. Skottfelt, Ben Dryer, George Seabroke, José Hernández e Andrew D. Holland. "Gaia CCDs: charge transfer inefficiency measurements between five years of flight". In X-ray, Optical, and Infrared Detectors for Astronomy IX, editado por Andrew D. Holland e James Beletic. SPIE, 2020. http://dx.doi.org/10.1117/12.2562162.

Texto completo da fonte

Estilos ABNT, Harvard, Vancouver, APA, etc.

Hou, Rui, Shanghong Zhao, Zhoushi Yao, Jie Xu e Xiaofeng Jiang. "Analysis of charge transfer inefficiency of CCD equipment under proton radiation". In 2011 International Conference on Electronics and Optoelectronics (ICEOE). IEEE, 2011. http://dx.doi.org/10.1109/iceoe.2011.6013232.

Texto completo da fonte

Estilos ABNT, Harvard, Vancouver, APA, etc.

Grant, Catherine E., Mark W. Bautz, Steven E. Kissel e Beverly LaMarr. "A charge-transfer-inefficiency correction model for the Chandra advanced-CCD imaging spectrometer". In SPIE Astronomical Telescopes + Instrumentation, editado por Andrew D. Holland. SPIE, 2004. http://dx.doi.org/10.1117/12.550628.

Texto completo da fonte

Estilos ABNT, Harvard, Vancouver, APA, etc.

Sopczak, Andre, Salim Aoulmit, Khaled Bekhouche, Chris Bowdery, Craig Buttar, Chris Damerell, Dahmane Djendaoui et al. "Modeling of charge transfer inefficiency in a CCD with high-speed column parallel readout". In 2008 IEEE Nuclear Science Symposium and Medical Imaging conference (2008 NSS/MIC). IEEE, 2008. http://dx.doi.org/10.1109/nssmic.2008.4774902.

Texto completo da fonte

Estilos ABNT, Harvard, Vancouver, APA, etc.

Relatórios de organizações sobre o assunto "Charge Transfert Inefficiency"

Norelli, John L., Moshe Flaishman, Herb Aldwinckle e David Gidoni. Regulated expression of site-specific DNA recombination for precision genetic engineering of apple. United States Department of Agriculture, março de 2005. http://dx.doi.org/10.32747/2005.7587214.bard.

Texto completo da fonte

Resumo:

Objectives: The original objectives of this project were to: 1) evaluate inducible promoters for the expression of recombinase in apple (USDA-ARS); 2) develop alternative selectable markers for use in apple to facilitate the positive selection of gene excision by recombinase (Cornell University); 3) compare the activity of three different recombinase systems (Cre/lox, FLP/FRT, and R/RS)in apple using a rapid transient assay (ARO); and 4) evaluate the use of recombinase systems in apple using the best promoters, selectable markers and recombinase systems identified in 1, 2 and 3 above (Collaboratively). Objective 2 was revised from the development alternative selectable markers, to the development of a marker-free selection system for apple. This change in approach was taken due to the inefficiency of the alternative markers initially evaluated in apple, phosphomannose-isomerase and 2-deoxyglucose-6-phosphate phosphatase, and the regulatory advantages of a marker-free system. Objective 3 was revised to focus primarily on the FLP/FRT recombinase system, due to the initial success obtained with this recombinase system. Based upon cooperation between researchers (see Achievements below), research to evaluate the use of the FLP recombinase system under light-inducible expression in apple was then conducted at the ARO (Objective 4). Background: Genomic research and genetic engineering have tremendous potential to enhance crop performance, improve food quality and increase farm profits. However, implementing the knowledge of genomics through genetically engineered fruit crops has many hurdles to be overcome before it can become a reality in the orchard. Among the most important hurdles are consumer concerns regarding the safety of transgenics and the impact this may have on marketing. The goal of this project was to develop plant transformation technologies to mitigate these concerns. Major achievements: Our results indicate activity of the FLP\FRTsite-specific recombination system for the first time in apple, and additionally, we show light- inducible activation of the recombinase in trees. Initial selection of apple transformation events is conducted under dark conditions, and tissue cultures are then moved to light conditions to promote marker excision and plant development. As trees are perennial and - cross-fertilization is not practical, the light-induced FLP-mediated recombination approach shown here provides an alternative to previously reported chemically induced recombinase approaches. In addition, a method was developed to transform apple without the use of herbicide or antibiotic resistance marker genes (marker free). Both light and chemically inducible promoters were developed to allow controlled gene expression in fruit crops. Implications: The research supported by this grant has demonstrated the feasibility of "marker excision" and "marker free" transformation technologies in apple. The use of these safer technologies for the genetic enhancement of apple varieties and rootstocks for various traits will serve to mitigate many of the consumer and environmental concerns facing the commercialization of these improved varieties.

Estilos ABNT, Harvard, Vancouver, APA, etc.

Oferecemos descontos em todos os planos premium para autores cujas obras estão incluídas em seleções literárias temáticas. Contate-nos para obter um código promocional único!