Literatura científica selecionada sobre o tema "Cellules spermatiques"
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Artigos de revistas sobre o assunto "Cellules spermatiques"
Hryniewiecka-Szyfter, Zofia, Elzbieta Gabala e Adam Babula. "THE ROLE OF SERTOLI CELLS IN THE ORGANIZATION OF SPERM BUNDLES IN THE TESTIS OF SADURIA ENTOMON (LINNAEUS, 1758) (ISOPODA, VALVIFERA)". Crustaceana 72, n.º 9 (1999): 1067–78. http://dx.doi.org/10.1163/156854099504022.
Texto completo da fonteMITCHELL, V., S. BRABANT, I. KOSCINSKI, E. HERMAND e J. RIGOT. "La proacrosine, un marqueur acrosomal pour la détection des cellules spermatiques dans le sperme éjaculé de patients azoospermes". Gynécologie Obstétrique & Fertilité 32, n.º 9 (setembro de 2004): 779–84. http://dx.doi.org/10.1016/s1297-9589(04)00203-6.
Texto completo da fonteTeses / dissertações sobre o assunto "Cellules spermatiques"
Gavin-Plagne, Lucie. "Cryoconservation de cellules spermatiques et de cellules souches pluripotentes de mammifères dans un milieu synthétique et chimiquement défini". Thesis, Lyon, 2018. http://www.theses.fr/2018LYSE1197/document.
Texto completo da fonteNowadays, reproductive (embryos, sperm, oocytes) and somatic (fibroblasts and pluripotent stem cells) resources are cryopreserved in media containing animal-derived products (serum, egg yolk, milk). Using these products raises sanitary (risk of contamination) as well as scientific concerns (reproducibility limits due to the variability of their composition). This study aims to replace animal derived-product in assessing the effect of a synthetic and chemically defined medium, STEMALPHA.CRY03® (Stem Alpha, France), on the cryopreservation of ovine and bovine sperm, and on rabbit pluripotent stem cells. First, a physical approach permitted to study the cooling rates and the characterization of thermodynamic properties of the freezing media. The differential scanning calorimetry allowed us to define their phase transition temperatures (crystallization temperature, melting temperature and enthalpy variation of crystallization, proportional to the amount of crystallized ice). Second, a biological approach was used for the cryopreservation of bovine and ovine sperm, as well as rabbit pluripotent stem cells. Flow cytometry and computer- assisted sperm analyses showed that STEMALPHA.CRY03® impaired bovine sperm, compared to a medium containing animal derived-product. These last results were confirmed in ovine species. Nevertheless, artificial insemination by laparoscopy (n = 270 ewes) counteracts this impairment and allowed an average pregnancy rate of 70 %. Moreover, without any additive in the freezing medium, a similar pregnancy rate was obtained. The study of pluripotent gene expression profile, and analyses of viability and growth rates for the cryopreservation of rabbit pluripotent stem cells confirmed that synthetic media, STEMALPHA.CRY03® (with 4, 5 or 10 % of cryoprotectant) and CryoStor® CS10 (containing 10 % of cryoprotectant) were more efficient than serum-based media. We demonstrate that it is possible to cryopreserve sperm cells and pluripotent stem cells in synthetic and chemically defined media. 0ur results confirmed the interest of a standardized approach for cryopreservation procedures of genetic resources in mammals. This work meets the needs of cryobanking activities (quality policy) and of the regulation development within the framework of international trade
Riou, Cindy. "Intéraction des spermatozoïdes avec l'épithélium du tractus génital femelle : réservoirs spermatiques, protéomique, et fertilité". Thesis, Tours, 2017. http://www.theses.fr/2017TOUR4051/document.
Texto completo da fonteIn avian species, the sperm storage mainly takes place in uterovaginal sperm storage tubules (SST) during several weeks. Mechanisms implied in this process are not fully understood. The effect of artificial insemination (AI) has been evaluated on the uterine fluid (UF) proteomic composition, and on SST candidate proteins, from hens exhibiting long (F+) or short (F-) sperm storage duration. Long sperm storage duration was associated with the relative abundance in UF after AI of proteoglycans (TSKU), proteoglycan binding proteins (HAPLN3, FN1, VTN), lipid transporters (VTG1, VTG2, APOA1, APOA4, APOH), and eggshell matrix proteins (OCX32). In contrast, poor sperm storage ability was associated with the regulation of immune factors (PIGR, immunoglobulins), pro-inflammatory factors (LTA4H), proteases (XPNPEP1), chaperone (HSPA8), mucins (MUC5AC, MUC5B), and ovalbumin related protein Y (OVALY). At the level of SST, eggshell matrix proteins (OC-116, OCX36, OC-17) were identified in SST cells and lumen. Long sperm storage duration was associated in SST with the luminal secretion of Glc/GlcNAc residues, ANXA4 apical mobilization, and non-activation of metabolic pathway implying PIGR, HSPA8, and ANXA5. In conclusion, the proteomic composition of UF and SST require specific regulation after insemination, most probably to guarantee the success of sperm storage process
Pipart, Perrine. "Biopolymères pour l'insémination artificielle". Electronic Thesis or Diss., Sorbonne université, 2024. http://www.theses.fr/2024SORUS217.
Texto completo da fonteReproductive biotechnologies using artificial insemination are widely spread in the primary livestock sector, particularly for species such as cattle, pigs, horses, and poultry. Artificial insemination allows for the control of fertility and the improvement of herd genetics without the constraints associated with moving animals. It makes it possible to fertilize many females with the semen of a single male. As the offspring inherit part of the male's genetic makeup, the male is selected based on his qualities, such as muscle development in the case of a beef breed bull. Other advantages of artificial insemination include cost reduction through a decrease in the population of male breeders, the limitation of health risks (sexually transmitted diseases), and the control of birthing periods. In several species, the cryogenic preservation of semen has become a common practice, facilitating the conservation and international distribution of animal genetic resources. In this context, the use of biopolymers could offer significant benefits. Their ability to form gels or viscous media and stabilize suspensions could enhance the effectiveness of animal semen preservation
Chalbi, Mariem. "Rôle de la protéine spermatique Izumo1 dans l'interaction gamétique chez le murin". Phd thesis, Université Pierre et Marie Curie - Paris VI, 2013. http://tel.archives-ouvertes.fr/tel-00986489.
Texto completo da fonteIzumo1 est une protéine transmembranaire, membre de la famille des protéines Izumo et de la superfamille des Immunoglobulines. Malgré son rôle clé dans l'interaction gamétique, ses propriétés fonctionnelles en tant que protéine d'adhésion, de fusion ou ayant la capacité d'organiser des réseaux comprenant des protéines de fusion n'est pas encore élucidé.
Dans cette étude nous nous sommes intéressés au rôle d'Izumo1 dans l'interaction gamétique.
Pour cela, nous avons généré deux variantes exogènes de la protéine Izumo1et les avons surexprimées à la membrane de plusieurs lignées cellulaires. L'interaction entre ces cellules exprimant la protéine et l'ovocyte a été analysée au moyen d'une technique de micromanipulation de cellules couplée à l'imagerie confocale. Nous avons ainsi mis en évidence une forte adhésion entre les cellules exprimant Izumo1 et les ovocytes et nous avons quantifié sa cinétique. Nous avons également généré le domaine extracellulaire recombinant afin de déterminer si Izumo1 seule était capable de se lier directement à l'ovocyte et comment. Nous avons sondé au moyen d'une technique fine de mesure de forces, le biomembrane force probe, l'interaction entre un Izumo1 unique et l'ovocyte. Ces expériences permettent de confirmer que c'est bien Izumo1 et non un partenaire qui est responsable de la forte adhésion entre cellules et ovocytes. Par observation en microscopie confocale d'un ovocyte interagissant avec des cellules surexprimant Izumo1-GFP, nous avons observé l'accumulation d'Izumo1-GFP dans la zone d'adhésion. Le fait que ce processus se reproduise lorsque plusieurs cellules adhèrent à l'ovocyte suggère qu'Izumo1 possède une molécule partenaire largement exprimée à la membrane de l'ovocyte avec laquelle il interagit pour créer de l'adhésion.
Congras, Annabelle. "Analyse de la méthylation de l'ADN spermatique et développement de cellules pluripotentes induites chez des verrats infertiles porteurs ou non de remaniements chromosomiques". Toulouse 3, 2014. http://thesesups.ups-tlse.fr/2585/.
Texto completo da fonteFertility issues concern both humans, affecting a growing part of the population, and farm animals including pigs in which they slow down the diffusion of agronomical traits of interest. In this project, we focused on two mechanisms linked to infertility: alterations in gametic DNA methylation and chromosomal rearrangements. We first observed that the methylation level of spermatic DNA is conserved between three mammalian species, both at the global and local level as well as between fertile and infertile boars. A specific increase in DNA methylation in the GNAS locus was identified, as well as a deregulation of its expression in some boars with low quality semen, linking for the first time hypermethylation of this region and male infertility in mammals. We then chose to produce induced pluripotent stem cell lines (iPSCs) derived from fibroblasts of infertile boars carrying chromosomal rearrangements, as a tool for studying their differentiation towards the germ cell lineage. Cell lines derived from an azoospermic t(Y;14) boar harbor several characteristics of pluripotency: expression of specific genes, a cell cycle resembling the one of embryonic stem cells, and an ability to evolve into the naïve state in adapted culture medium. However they revealed a poor differentiation potential and a genomic instability increasing with passaging that we associated with the use a an integrative reprogramming technique. The use of a non-integrative technique demonstrated that the cell lines obtained with this method did not harbor this instability. Their preliminary characterization may be predictive of production of more stable cell lines gathering more characters of pluripotency