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Ryan, Michael A. "Fearful Spirits, Reasoned Follies: The Boundaries of Superstition in Late Medieval Europe by Michael D. Bailey". Catholic Historical Review 101, n.º 3 (2015): 635–36. http://dx.doi.org/10.1353/cat.2015.0137.
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Bell, James B. "The Reverend Jacob Bailey, Maine Loyalist: For God, King, Country, and for Self by James S. Leamon (review)". Catholic Historical Review 99, n.º 2 (2013): 392–93. http://dx.doi.org/10.1353/cat.2013.0099.
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Smith, Brandon R., e Lailiang Cheng. "Photoprotective Mechanisms of `Concord' Grape Leaves in Relation to Iron Supply". Journal of the American Society for Horticultural Science 130, n.º 3 (maio de 2005): 331–40. http://dx.doi.org/10.21273/jashs.130.3.331.
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The objective of this study was to quantify how photoprotective mechanisms in the leaves of `Concord' grapevines (Vitis labruscana Bailey) respond to a range of iron (Fe) supply. Own-rooted, 1-year-old container-grown vines were fertigated twice weekly for 11 weeks with a complete nutrient solution containing 1, 10, 20, 50, or 100 μm Fe from ferric ethylenediamine di (o-hydroxyphenylacetic) acid (Fe-EDDHA). Leaf total Fe content did not increase in response to Fe supply; however, “active” Fe (extracted with 2,2′-dipyridyl) and chlorophyll (Chl) increased on a leaf area basis as applied Fe increased. At the lowest active Fe level, leaf absorptance and the efficiency of excitation transfer (Fv′/Fm′) was lower, and nonphotochemical quenching (NPQ) was significantly greater. Photosystem II (PSII) quantum efficiency decreased curvilinearly, and the proportion of PSII reaction centers in the open state (qP) decreased linearly as active Fe content decreased. On a Chl basis, the xanthophyll cycle pool size [violaxanthin (V) + antheraxanthin (A) + zeaxanthin (Z)], lutein, and β-carotene increased curvilinearly as active Fe decreased, and neoxanthin (Neo) increased at the lowest Fe level. On a leaf area basis, as active Fe decreased, V+A+Z and β-carotene decreased curvilinearly, and lutein and Neo decreased linearly. At noon, conversion of V to A and Z increased as active Fe decreased. On a Chl basis, activities of antioxidant enzymes superoxide dismutase (SOD), monodehydroascorbate reductase (MDAR), and dehydroascorbate reductase (DHAR) increased curvilinearly, and glutathione reductase (GR) activity increased linearly as active Fe levels declined. Ascorbate peroxidase (APX) and catalase (CAT), on a Chl basis, were relatively constant. On a leaf area basis, a decrease in active Fe increased SOD and MDAR activity, whereas APX, CAT, DHAR and GR activity decreased. Antioxidant metabolites ascorbate (AsA), dehydroascorbate (DAsA), reduced glutathione (GSH) and oxidized glutathione (GSSG) also increased in response to Fe limitation when expressed on a Chl basis, whereas on a leaf area basis AsA and DAsA decreased and GSH increased curvilinearly. The GSH:GSSG ratio increased as active Fe declined, whereas the AsA:DAsA ratio did not change. In conclusion, both photoprotective mechanisms, xanthophyll cycle-dependent thermal dissipation and the ascorbate-glutathione antioxidant system, are enhanced in response to Fe deficiency to cope with excess absorbed light. In a low soil pH tolerant species such as V. labruscana, the foliar antioxidant system was upregulated in response to excess absorbed light from Fe deficiency-induced chlorosis, and there was no evidence of an increase in oxidative stress from high rates of applied Fe-EDDHA.
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Carpenter, Faedra Chatard, e Moya Bailey. "An Interview with Moya Bailey". Callaloo 29, n.º 3 (2006): 753–60. http://dx.doi.org/10.1353/cal.2006.0132.
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Raghavan, Srinivas, e Gary Perlman. "The Difference of Preference versus Performance can Differ for Concurrent versus Retrospective Ratings". Proceedings of the Human Factors and Ergonomics Society Annual Meeting 44, n.º 37 (julho de 2000): 617–20. http://dx.doi.org/10.1177/154193120004403719.
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Several studies have found differences between subjective preference ratings and objective performance measures. Bailey [Bailey 93] summarizes several, and argues for separate treatment of these concepts. Our results in a multifactor multivariate experiment support Bailey's contention, but adds a new dimension of concern: the use of concurrent versus retrospective subjective ratings. The presentation here will focus on the relationship of performance and concurrent versus retrospective preference ratings. Retrospective ratings may represent users' lasting impressions of a system after a trial use, but may not be good predictors of performance. Concurrent ratings of confidence of accuracy were found, in this study, to be better predictors performance. We offer recommendations about how to make the best use of these different evaluation measures, particularly when they differ.
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Tice, Troy J. ""Containing Heresy and Errors": Thomas of Bailly and the Condemned Extracts of the Mirror of Simple Souls". Catholic Historical Review 104, n.º 4 (2019): 614–35. http://dx.doi.org/10.1353/cat.2019.0013.
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Guymer, Gordon P. "Short communication: Austrobaileya C.T. White honors F.M. Bailey". Austrobaileya: A Journal of Plant Systematics 9, n.º 4 (2016): 600. http://dx.doi.org/10.5962/p.364346.
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ROIGÉ, ROBERT CASAS. "POR AL CEL (2015) D’AXEL BAIGET I MARTA TRILL: ESPELEOLOGIA I RESISTÈNCIES DE LA MEMÒRIA CONFLICTIVA". Catalan Review 38, n.º 1 (junho de 2024): 101–15. http://dx.doi.org/10.3828/catr.38.6.
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When historian Axel Baiget and journalist Marta Trill began to investigate the consequences of the fascist bombings during the Spanish Civil War of the town of La Selva del Camp (Tarragona), they automatically thought of making a documentary. Por al cel (2015) was conceived as an audiovisual tale that was both oral and choral, in which witnesses would reconstruct their own experience without the intervention of Baiget and Trill’s voice-over. The present article analyzes the many explicit and implicit reticences that Baiget and Trill dealt with throughout the documentary’s filming. During that process, the authors reopened and explored some abandoned wartime bomb shelters. To reach some of those spaces was almost as complicated as accessing the accounts of many of the neighbors of la Selva who, from the very beginning, resisted participation in the documentary, refusing to revisit the buried collective/ conflictual memory. On the other hand, Por al cel allowed those who finally decided to participate to share their stories publicly for the first time, even though this format, based on a principle of non-intervention, opened the door for the emergence of lapses and reconstructions of the past.
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Bailey, D. J., M. C. Stennett, B. Ravel, D. Grolimund e N. C. Hyatt. "Correction: Synthesis and characterisation of brannerite compositions (U0.9Ce0.1)1−xMxTi2O6 (M = Gd3+, Ca2+) for the immobilisation of MOX residues". RSC Advances 11, n.º 6 (2021): 3685. http://dx.doi.org/10.1039/d0ra90131h.
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Correction for ‘Synthesis and characterisation of brannerite compositions (U0.9Ce0.1)1−xMxTi2O6 (M = Gd3+, Ca2+) for the immobilisation of MOX residues’ by D. J. Bailey et al., RSC Adv., 2018, 8, 2092–2099. DOI: 10.1039/C7RA11742F.
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But, Roxanne. "“He said he was going on the scamp”: Thieves’ cant, enregisterment and the representation of the social margins in the Old Bailey Sessions Papers". Journal of Historical Sociolinguistics 3, n.º 2 (26 de outubro de 2017): 151–71. http://dx.doi.org/10.1515/jhsl-2017-1001.
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AbstractThis paper investigates how cant is used to represent the social margins in the Old Bailey Sessions Papers. Cant refers to the special vocabulary that is associated with and used by people living on the margins such as thieves and prostitutes. Little work has been conducted on the use of this language in courtroom texts. Using a historical pragmatic framework, evidence of the actual occurrence of cant as well as metalinguistic evidence was generated through lexical keyword searches in the Old Bailey Proceedings Online. Then the use of marginal vocabulary was examined more closely in extracts from the Sessions Papers using historical discourse analysis. This paper argues that cant is enregistered and that the courtroom recorder and the editor of the Session Papers reproduced the cant language to highlight and draw attention to the maliciousness and culpability of those who were accused in court. Linguistic techniques such as glossing and metalinguistic commentary were inserted to foreground the cant terms in the text. In addition, this historical discourse analysis sheds light on how the witnesses in the courtroom make strategic use of cant terms to portray the defendant in a negative light.
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Bailey, Josephine. "Back to School: a Student Insight into LIS Qualifications, the LIS Sector and What it can Offer to New Professionals". Legal Information Management 16, n.º 1 (março de 2016): 49–53. http://dx.doi.org/10.1017/s1472669616000128.
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AbstractJosephine Bailey, the inaugural recipient of BIALL's Professional Studies Bursary, provides a student's perspective on current LIS qualifications, what they can offer to individuals hoping to enter the profession and the challenges facing new professionals today. Her observations and comments are based on her experiences of the MA course in Librarianship and Information Services Management which is offered by the University of Sheffield and is currently ranked as the top university for Librarianship and Information Management.
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Kelly, Laura, e Cinthya Bolanos. "From Outreach to Translanguaging: Developing a Bilingual Storytime". Children and Libraries 18, n.º 3 (25 de setembro de 2020): 28. http://dx.doi.org/10.5860/cal.18.3.28.
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A college student shows the illustrations of the bilingual book What Can You Do with a Rebozo? She turns to several pages that she has marked, shows the pictures to children gathered for bilingual storytime, and talks about each picture in English. The student stops on the page that describes a baile, or dance, that the child in the book does with her mamá’s rebozo, or brightly colored shawl. The student reads this page in Spanish and asks the children if they would like to dance with their own rebozos. The children excitedly say they would, and the student passes out play scarves. The children jump, clap, stomp, and sing along with their rebozos while dancing to an English song.
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Tehan, Claire. "Bailey House: To Live as Long as You Can, produced by Alain Karner". Hospice Journal 7, n.º 4 (dezembro de 1991): 86–87. http://dx.doi.org/10.1080/0742-969x.1991.11882715.
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Fedoroff, J. Paul, Susan Curry, Karolina Müller, Rebekah Ranger, Peer Briken e John Bradford. "Evidence That Arousal to Pedophilic Stimuli Can Change: Response to Bailey, Cantor, and Lalumière". Archives of Sexual Behavior 44, n.º 1 (3 de dezembro de 2014): 259–63. http://dx.doi.org/10.1007/s10508-014-0441-9.
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Jinan Abdulla Shafiq. "Dysfunctional Families: A Study in Flannery O’Connor’s “A Good Man is hard to Find” and “Good Country People”". Journal of the College of Basic Education 23, n.º 98 (26 de dezembro de 2022): 11–20. http://dx.doi.org/10.35950/cbej.v23i98.8642.
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Flannery O’Connor is one of the great short stories writers of the twentieth century. She wrote about southern life. She reflects a theme that became well-known in most of the modern works, which is the dysfunctional family. It is the sense of vanity and disobedience that leads to dysfunctional families that became a modern age phenomena.
In “A Good Man is Hard to Find” one can find the self- reliant individualو the Baily, his kids and the grandmother. No one seems to pay any attention to the grandmother’s fears or suggestions, it is only her cat that gives her the sense she is not alone. In ‘Good Country People’ O’Connor’s other short story that deals with a prideful individual ‘Joy Hopefull’, with her fits of rage, she reveals an image of dysfunctional family. Challenging her mother who treats her as still a young child, Joy changes her name to Hulga. Breaking with her mother and the traditions, Hulga became an easy prey to a deceiving Bible salesman.
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Trindade, Milena Guirelli. "O gênero morfológico do substantivo cal". Entrepalavras 12, n.º 2 (26 de dezembro de 2022): 117. http://dx.doi.org/10.22168/2237-6321-22508.
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O objetivo do presente artigo consiste em investigar o fenômeno do gênero morfológico do substantivo cal, que frequentemente é tomado como pertencente ao feminino pelas gramáticas, ao passo que costuma ser empregado tanto no feminino quanto no masculino pelos falantes de português. Desse modo, uma vez que cal consiste em uma das poucas exceções de palavras terminadas em -l que não são masculinas (SAID ALI, 1923, p. 63), realizamos uma análise diacrônica para verificar as motivações para a oscilação de gênero desse vocábulo. Para isso, consideramos as definições de hesitação e mudança de gênero formuladas por Gouveia (2005) e investigamos a palavra desde a sua correspondente no grego antigo e no latim, nos baseando em autores como Bailly (1950) e Gaffiot (1934), além de verificar como se deu a fixação de gênero desse item lexical em outras línguas românicas, como o espanhol e o galego.
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Wang, B., Xian Li Liu, Cai Xu Yue, J. C. Du, L. Xu e X. Q. Chen. "Study on NC Machining Technology for Integral Impeller of Pelton Turbine". Advanced Materials Research 102-104 (março de 2010): 861–65. http://dx.doi.org/10.4028/www.scientific.net/amr.102-104.861.
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Integral production technology of impulse turbine runner effectively prevents phenomenon of bailer broken when generation unit working, and then improving its service life. Based on comprehensive application of vibration inhibition technology and machining optimization path CAD/CAM technology, the high-speed plunge milling process is forwarded in this paper under the condition of big slenderness ratio and be able to present processing vibration. By using matching principle of tool connection point surface smoothing processes and the helix curved surface processing method this process successfully solves the problem of interfering point state complex in finishing plunge milling impulse water turbine whole runner and it achieves high active finish machining by three-axis NC process. This technology has also successfully been used in the power station whole runner manufacturing process in HPE group.
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Woodard, Alyssa J., Jonathan R. Schultheis, Katherine M. Jennings, Alex L. Woodley e David H. Suchoff. "Horizontal Planting Orientation Can Improve Yield in Organically Grown Sweetpotato". HortScience 59, n.º 1 (janeiro de 2024): 36–42. http://dx.doi.org/10.21273/hortsci17352-23.
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Sweetpotato [Ipomoea batatas (L.) Lam.] is one of North Carolina’s (USA) most important organic commodity crops; however, yields tend to be less when compared with conventionally produced sweetpotato. Standard field establishment uses unrooted stem cuttings that are transplanted vertically in the soil. Producers in other countries typically use other planting orientations, including cuttings transplanted horizontally. Empirical evidence from North Carolina, USA, sweetpotato producers suggests that a horizontal orientation may improve yields. An organically managed field study using ‘Monaco’ sweetpotato was conducted in 2020 and 2021 in Bailey, NC, USA. The study evaluated stem cutting planting orientations (vertical, sleeve, horizontal), stem cutting length (25 cm and 38 cm), and harvest time (early or late) in a full-factorial randomized complete block design. In 2020, marketable yields were 16% greater for the horizontal orientation compared with the vertical orientation, with intermediate yields using the sleeve attachment. However, in 2021, there were no differences in marketable yield among planting orientations. In both years, US No. 1–grade yields were significantly greater when cuttings were planted horizontally compared with vertically, with an average increase of 18%. Delaying harvest until ∼126 days is recommended to increase yields for ‘Monaco’, regardless of planting orientation. This study provides evidence that a horizontal planting orientation could increase premium root yields and improve land-use efficiency for organically produced sweetpotatoes.
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Tai, Kulsum, Sanjima Pal, Julie Bérubé, Iris Kong, Adam Hoffman, Swneke Bailey, Aki Kirbizakis et al. "Abstract 5959: Dissecting the stromal drivers of gastroesophageal adenocarcinoma chemoresistance". Cancer Research 83, n.º 7_Supplement (4 de abril de 2023): 5959. http://dx.doi.org/10.1158/1538-7445.am2023-5959.
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Abstract Gastroesophageal adenocarcinoma (GEA) is the fastest rising cancer in North America. Over the course of five years, the survival rate is <20%, creating an urgent need for appropriate treatments against GEA. Currently, providing patients with peri-operative systemic docetaxel triplet-based chemotherapy (DCF or FLOT) is the most effective approach to treat GEA. Despite this, for 50% of patients that do present an initial response to therapy, the tumor returns due to pre-existing or newly acquired resistance (i.e., chemoresistance) by the cancer. Researchers have shifted their focus to the tumor microenvironment (TME) as one of the factors influencing chemoresistance in patients. The TME is composed of tumor cells, immune cells and their secreted products, as well as fibroblasts. The components of the TME have been shown to interact with one another to influence tumor growth and progression. Fibroblasts are wound-healing cells that can be transformed into cancer-associated fibroblasts (CAFs) in response to stress and the release of inflammatory products. CAFs are the most abundant cells in the TME, yet their role in the chemoresponse of GEA is still unclear. Previous studies on other cancer types demonstrated that CAF expression is distinct between chemoresistant and chemo-sensitive tumors and certain CAF subpopulations may confer this resistance. This project will investigate the role of CAFs in the chemo-response of GEA using patient-derived organoids (PDOs) and CAFs from >200 GEA patients. A single-cell RNA sequencing atlas developed from >30 GEA DCF- or FLOT-treated patient samples will be used to identify CAF markers and targetable processes. CAF sub-populations will then be elucidated and correlated to tumor response. IF, FACS, and ELISA will be performed for subsequent CAF marker validation and characterization. Ex vivo drug testing with DCF or FLOT will be conducted on PDO-CAF co-cultures to recapitulate their drug response. Citation Format: Kulsum Tai, Sanjima Pal, Julie Bérubé, Iris Kong, Adam Hoffman, Swneke Bailey, Aki Kirbizakis, Sui Huang, Michael Strasser, David Gibbs, Nicholas Bertos, Veena Sangwan, Lorenzo Ferri. Dissecting the stromal drivers of gastroesophageal adenocarcinoma chemoresistance [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2023; Part 1 (Regular and Invited Abstracts); 2023 Apr 14-19; Orlando, FL. Philadelphia (PA): AACR; Cancer Res 2023;83(7_Suppl):Abstract nr 5959.
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McGregor, Hugh W., Jordan O. Hampton, Danielle Lisle e Sarah Legge. "Live-capture of feral cats using tracking dogs and darting, with comparisons to leg-hold trapping". Wildlife Research 43, n.º 4 (2016): 313. http://dx.doi.org/10.1071/wr15134.
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Context Predation by feral cats is a key threatening process to many species of native Australian wildlife. Unfortunately, cats are difficult to capture using standard trapping techniques, limiting the potential to conduct research on their ecology and impacts. Aims We present an alternative capture method: remote chemical immobilisation after tracking with trained dogs. We also compare capture rates to a concurrent soft-jaw leg-hold trapping program. Methods We used dogs to capture cats detected by spotlighting at night, and also recaptured cats fitted with telemetry collars during the day. Cats were either bailed on the ground or treed and then hand-netted, or chemically immobilised using darts shot from a CO2-powered dart rifle, loaded with tiletamine–zolazepam at ~6 mg kg–1. Factors affecting the success rate of capturing cats using dogs were assessed. Efficiency in terms of cats captured per person-hours of fieldwork were compared using trained dogs versus leg-hold trapping. Key results We attempted 160 cat captures using the tracking dogs with 114 of those being successful. There were no mortalities or debilitating physical injuries associated with chemical immobilisation; however, sedated cats had prolonged recoveries (>4 h). Capture success with the tracking dogs increased as the dogs gained experience. Capture success rates per person-hour of fieldwork were four times greater using spotlighting with tracking dogs than using leg-hold traps. The success rate of recaptures using dogs was 97%. Conclusions The use of trained tracking dogs proved an effective method for capturing feral cats. The method had a much higher success rate than live-trapping with leg-hold traps, took less effort (in terms of person-hours) and caused less physical injuries than did leg-hold traps. However, substantial setup costs and time are required, which are discussed. Implications Using these methods could improve efficiency and outcomes when catching feral cats, and enable more data per individual cat to be collected than otherwise.
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Cronin, Stephen B. "(Invited) Enhanced Photocatalysis on TiO2-Passivated III-V Compound Semiconductors and 2D Material Heterostructures for Water Splitting and CO2 Reduction". ECS Meeting Abstracts MA2023-01, n.º 30 (28 de agosto de 2023): 1802. http://dx.doi.org/10.1149/ma2023-01301802mtgabs.
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We report enhanced photocatalysis for H2 evolution and CO2 reduction using TiO2-passivated InP and GaAs photocathodes.1-4 The TiO2 layer makes the InP semiconductor photochemically stable. This represents a major step forward in photocatalysis, which has typically been limited to metal oxide materials. In addition to making these surfaces stable, the TiO2 film, deposited by atomic layer deposition (ALD), also provides a substantial enhancement in the efficiency of H2 evolution. We find that passivating GaAs with just a few nm of TiO2 produces a shift in the onset potential of H2 evolution by +0.35 V at 1 mA/cm2 and enhances the photocurrent by 32-fold over bare GaAs (at 0 V vs. RHE). Here, thinner TiO2 films produce a larger enhancement than thicker films, which correlates with the higher density of O-vacancies (i.e., Ti3+ surface states) observed in these thinner films using X-ray photoemission spectroscopy (XPS). While TiO2 films 1-5nm thick produce large enhancements, no enhancement is observed for TiO2 thicknesses above 10 nm, which are crystalline and, therefore, considerably more insulating than thinner amorphous TiO2 films. Using transient reflectance spectroscopy (TRS), we measure the photoexcited carrier dynamics in a GaP/TiO2 photoelectrode, as well as the electrostatic field dynamics at this semiconductor-liquid interfaces in situ under various electrochemical potentials.5 Here, the electrostatic fields at the surface of the semiconductor are measured via Franz−Keldysh oscillations (FKO). These spectra reveal that the nanoscale TiO2 protection layer enhances the built-in field and charge separation performance of GaP photoelectrodes. We also demonstrated plasmon-resonant enhancement of photocatalysis on monolayer WSe2.6 In addition, we measured the stacking dependence and resonant interlayer excitation of monolayer WSe2/MoSe2 heterostructures for photocatalytic energy conversion.7 Qiu, J., G. Zeng, M. Ge, S. Arab, M. Mecklenburg, B. Hou, C. Shen, A.V. Benderskii and S.B. Cronin, Correlation of Ti3+ states with photocatalytic enhancement in TiO2-passivated p-GaAs. Journal of Catalysis, 337, 133 (2016). Qiu, J., G.T. Zeng, M.A. Ha, M.Y. Ge, Y.J. Lin, M. Hettick, B.Y. Hou, A.N. Alexandrova, A. Javey and S.B. Cronin, Artificial Photosynthesis on TiO2-Passivated InP Nanopillars. Nano Letters, 15, 6177-6181 (2015). Qiu, J., G.T. Zeng, M.A. Ha, B.Y. Hou, M. Mecklenburg, H.T. Shi, A.N. Alexandrova and S.B. Cronin, Microscopic Study of Atomic Layer Deposition of TiO2 on GaAs and Its Photocatalytic Application. Chemistry of Materials, 27, 7977-7981 (2015). Wang, Y. and S.B. Cronin, Performance Enhancement of TiO2-encapsulated Photoelectrodes Based on III–V Compound Semiconductors, in Ultrathin Oxide Layers for Solar and Electrocatalytic Systems. 2022, Royal Society of Chemistry. p. 103-134. Xu, Z.H., B.Y. Hou, F.Y. Zhao, Z. Cai, H.T. Shi, Y.W. Liu, C.L. Hill, D.G. Musaev, M. Mecklenburg, S.B. Cronin and T.Q. Lian, Nanoscale TiO2 Protection Layer Enhances the Built-In Field and Charge Separation Performance of GaP Photoelectrodes. Nano Letters, 21, 8017-8024 (2021). Chen, J., C.S. Bailey, Y. Hong, L. Wang, Z. Cai, L. Shen, B. Hou, Y. Wang, H. Shi, J. Sambur, W. Ren, E. Pop, and S.B. Cronin., Plasmon-Resonant Enhancement of Photocatalysis on Monolayer WSe2. ACS Photonics, 6, 787 (2019). Chen, J., C.S. Bailey, D. Cui, Y. Wang, B. Wang, H. Shi, Z. Cai, E. Pop, C. Zhou and S.B. Cronin, Stacking Independence and Resonant Interlayer Excitation of Monolayer WSe2/MoSe2 Heterostructures for Photocatalytic Energy Conversion. ACS Applied Nano Materials, DOI:10.1021/acsanm.9b01898 (2020).
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Nichols, R. A. B., B. M. Campbell e H. V. Smith. "Identification of Cryptosporidium spp. Oocysts in United Kingdom Noncarbonated Natural Mineral Waters and Drinking Waters by Using a Modified Nested PCR-Restriction Fragment Length Polymorphism Assay". Applied and Environmental Microbiology 69, n.º 7 (julho de 2003): 4183–89. http://dx.doi.org/10.1128/aem.69.7.4183-4189.2003.
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ABSTRACT We describe a nested PCR-restriction fragment length polymorphism (RFLP) method for detecting low densities of Cryptosporidium spp. oocysts in natural mineral waters and drinking waters. Oocysts were recovered from seeded 1-liter volumes of mineral water by filtration through polycarbonate membranes and from drinking waters by filtration, immunomagnetizable separation, and filter entrapment, followed by direct extraction of DNA. The DNA was released from polycarbonate filter-entrapped oocysts by disruption in lysis buffer by using 15 cycles of freeze-thawing (1 min in liquid nitrogen and 1 min at 65°C), followed by proteinase K digestion. Amplicons were readily detected from two to five intact oocysts on ethidium bromide-stained gels. DNA extracted from Cryptosporidium parvum oocysts, C. muris (RN 66), C. baileyi (Belgium strain, LB 19), human-derived C. meleagridis, C. felis (DNA from oocysts isolated from a cat), and C. andersoni was used to demonstrate species identity by PCR-RFLP after simultaneous digestion with the restriction enzymes DraI and VspI. Discrimination between C. andersoni and C. muris isolates was confirmed by a separate, subsequent digestion with DdeI. Of 14 drinking water samples tested, 12 were found to be positive by microscopy, 8 were found to be positive by direct PCR, and 14 were found to be positive by using a nested PCR. The Cryptosporidium species detected in these finished water samples was C. parvum genotype 1. This method consistently and routinely detected >5 oocysts per sample.
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Rivera, M. C., E. R. Wright e S. Carballo. "First Report of Colletotrichum gloeosporioides on Chinese Rose in Argentina". Plant Disease 84, n.º 12 (dezembro de 2000): 1345. http://dx.doi.org/10.1094/pdis.2000.84.12.1345b.
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Chinese rose (Hibiscus rosa-sinensis L.) is a shrub frequently planted in Argentina. In November 1999, dieback and anthracnose symptoms were detected on stems and leaves of plants cv. Hawaii cultivated in Buenos Aires. Disease prevalence was 50%. Pieces of infected tissues were surface-sterilized for 1 min in 2% NaOCl, plated on potato-dextrose agar and incubated at 24 ± 2°C. The isolate that was consistently recovered from diseased tissues was identified as Colletotrichum gloeosporioides (Penz.) Penz. and Sacc., based on morphological characteristics (1,2). Teleomorph stage was not observed. Inoculation for pathogenicity testing was carried out by spraying a conidial suspension (6.5 × 106 conidia per ml) on plants with previously punctured leaves and pruned stems. Inoculated plants with unwounded tissues, as well as noninoculated controls, were included. Five replications of each treatment were done. Plants were incubated in moist chambers at 24°C. Whitish areas of 0.3 to 0.5 cm diameter surrounded by a purple halo developed on all punctured leaves within 10 days. Stem blight and leaf drop were observed. The center of the lesions was covered by black acervuli 14 days after inoculation. Unwounded and noninoculated controls remained symptomless. The pathogen was reisolated from inoculated leaves, completing Koch's postulates. This is the first report of C. gloeosporioides causing disease on Chinese rose in Argentina. References: (1) J. A. Bailey and M. J. Jeger, eds. 1992. Colletotrichum. CAB International, Surrey, England. (2) B. C. Sutton. 1980. The Coelomycetes. CMI, Kew.
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Alvarez-Vargas, Daniela, David Braithwaite, Hugues Lortie-Forgues, Melody Moore, Sirui Wan, Elizabeth Martin e Drew Hal Bailey. "Hedges, mottes, and baileys: Causally ambiguous statistical language can increase perceived study quality and policy relevance". PLOS ONE 18, n.º 10 (26 de outubro de 2023): e0286403. http://dx.doi.org/10.1371/journal.pone.0286403.
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There is a norm in psychology to use causally ambiguous statistical language, rather than straightforward causal language, when describing methods and results of nonexperimental studies. However, causally ambiguous language may inhibit a critical examination of the study’s causal assumptions and lead to a greater acceptance of policy recommendations that rely on causal interpretations of nonexperimental findings. In a preregistered experiment, 142 psychology faculty, postdocs, and doctoral students (54% female), ages 22–67 (M = 33.20, SD = 8.96), rated the design and analysis from hypothetical studies with causally ambiguous statistical language as of higher quality (by .34-.80 SD) and as similarly or more supportive (by .16-.27 SD) of policy recommendations than studies described in straightforward causal language. Thus, using statistical rather than causal language to describe nonexperimental findings did not decrease, and may have increased, perceived support for implicitly causal conclusions.
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Mondelli, Giulliana, Heraldo Luiz Giacheti e Jorge Hamada. "Avaliação da contaminação no entorno de um aterro de resíduos sólidos urbanos com base em resultados de poços de monitoramento". Engenharia Sanitaria e Ambiental 21, n.º 1 (março de 2016): 169–82. http://dx.doi.org/10.1590/s1413-41520201600100120706.
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RESUMO Este artigo apresenta o monitoramento ambiental das águas subterrâneas do entorno do aterro de resíduos sólidos urbanos (RSU) de Bauru, São Paulo, Brasil. Paralelamente às diversas pesquisas realizadas na área durante o período de janeiro de 2003 a abril de 2008, foram construídos 14 poços de monitoramento em diferentes fases da investigação geoambiental, seguindo as normas vigentes na época e as necessidades observadas durante a interpretação dos resultados desta investigação, para confirmação da contaminação da área. As amostras foram coletadas com bailer , após o período de chuvas e de estiagem de cada ano. São apresentados os resultados obtidos ao longo do tempo para os seguintes parâmetros: nível d'água, pH, condutividade elétrica (CE), Ca2+, Cl, Fe total, DQO, NH4 +, Zn2+, Na+, K+, além da relação demanda biológica de oxigênio/demanda química de oxigênio (DBO/DQO). Os resultados do longo período de monitoramento permitiram a análise de cada parâmetro em função do que ocorria superficialmente ao aterro, assim como com características naturais do aquífero. Conclui-se que a pluma de contaminação varia conforme o ponto de disposição e as características dos resíduos depositados, cuja recuperação e atenuação deverão ocorrer com o encerramento das atividades do aterro.
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Eken, C., e E. Demirci. "First Report of Colletotrichum truncatum on Alfalfa in Turkey". Plant Disease 84, n.º 1 (janeiro de 2000): 100. http://dx.doi.org/10.1094/pdis.2000.84.1.100a.
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During the summer of 1997 and 1998, a pathogen identified as Colletotrichum truncatum (Schwein.) Andrus & W.D. Moore was isolated from lesions on stems of alfalfa (Medicago sativa L.) plants in Erzurum, Turkey. Typical symptoms on stems of mature plants were large, sunken, irregularly shaped black lesions. Twenty-eight cultures of C. truncatum were isolated from stem lesions. Acervuli containing spores and dark setae were observed within lesions. Conidia were hyaline, one-celled, falcate to nearly straight with a prominent clear area in the center of highly granular cytoplasm, and measured 16.3 to 20.6 × 3.1 to 4.5 μm. These morphological characteristics were consistent with the description of C. truncatum (1). The pathogenicity of two isolates was determined on alfalfa cv. Bilensoy. Alfalfa seedlings (6-week-old) were inoculated with a conidial suspension of the fungus (1.4 × 107 conidia per ml), incubated in a moist chamber for 3 days, and subsequently transferred to growth chambers maintained at 25°C with a 12-h photoperiod. Ten plants were inoculated with each isolate. Symptoms first appeared on stems 12 days after inoculation. Sunken, irregularly shaped black lesions occasionally girdled stems of plants inoculated with C. truncatum. Symptoms did not appear on stems of control plants inoculated with sterile distilled water. C. truncatum was reisolated from symptomatic tissue. This is the first report of C. truncatum on alfalfa from Turkey. Reference: (1) B. C. Sutton. 1992. Pages 1–27 in: Colletotrichum Biology, Pathology and Control. J. A. Bailey and M. J. Jeger, eds. CAB International, Wallingford, U.K.
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Walsh, R. McKinnon, Jarrid L. Jack, Austin E. Eades, Bailey A. Bye, Mariana T. Ruckert, Joseph Ambrose e Michael N. VanSaun. "Abstract 4460: Investigating a non-canonical role for SHP2 in pancreatic ductal adenocarcinoma metabolism and response to dietary modifications". Cancer Research 84, n.º 6_Supplement (22 de março de 2024): 4460. http://dx.doi.org/10.1158/1538-7445.am2024-4460.
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Abstract Pancreatic ductal adenocarcinoma (PDAC) is predicted to be the second leading cause of cancer death in the US by 2025, with rising incidence. Mutant KRAS in >90% of PDAC cases promotes activation of the MAPK pathway, yet targeting the MAPK pathway clinically has failed to improve patient outcomes. SHP2 canonically promotes KRAS activation and is essential for PDAC development in mouse models. Because targeting MAPK is insufficient, yet SHP2 loss blocks PDAC development, we hypothesized SHP2 has both MAPK dependent and independent functions. Initial studies revealed that SHP2 inhibition with SHP099 in murine KPC [K8484: KRASG12D/+, p53R172H/+, Pdx1Cre/+] cells required concentrations exceeding 50 μM to elicit a minimal ERK inhibition. This led us to predict that there may be a tumor suppressive mechanism of SHP2 inhibition that is KRAS/MAPK independent. We utilized available RNAseq data (NCBI GEO: PRJNA558508) of PDAC MiaPaCa2 cells treated with the MEK inhibitor, Trametinib, or SHP099 to compare the direct effects of inhibition on transcriptional outcomes. We identified inositol phosphate signaling as a candidate target, whose downstream consequence is Ca2+ flux. We measured IL-1β induced Ca2+ flux in a panel of human PDAC cells exposed to MEK or SHP2 inhibition. Trametinib treated cells showed no effect on Ca2+ flux, however, SHP099 led to a drastic depletion of free Ca2+ and response to stimulus. We then validated the Ca2+ response to IL-1β stimulus in Crispr-Cas9 SHP2-knockout K8484 cells. While we did not observe a difference in basal free calcium, we still found a reduction in response to IL-1β stimulus. Because Ca2+ is an important co-factor for mitochondrial metabolism, we then interrogated metabolic effects. We tested mitochondrial function using a Seahorse Mito-Stress test and found that while MEK inhibition failed to affect mitochondrial metabolism, SHP2 inhibition ablated it. A Glycolysis-Stress Test revealed that SHP2 inhibition led to glucose consumption primarily through anaerobic respiration. TMT-labeled phospho-proteomic analysis comparing SHP2KO and MEK inhibited K8484s revealed SHP2 dependent deficiencies in mitochondrial proteins, among others, involved in mitochondrial fission and lipid metabolism. Western diets have been shown to promote tumor progression in GEM models of PDAC, so we used KRASG12D/+, SHP2fl/fl or wt./wt, Ptf1aCre/+ (SKC and KC) mice to demonstrate that while diet-induced obesity affects KC tumor burden, the lack of SHP2 in SKCs prevents the diet associated discrepancy in tumor progression. Collectively, these data led us to conclude that while SHP2 has been studied as a contributor to KRAS signaling in PDAC, the outcomes of its role specifically on mitochondrial metabolism have yet to be appreciated or thoroughly investigated, opening the path for a new approach to its contribution to tumor progression. Citation Format: R. McKinnon Walsh, Jarrid L. Jack, Austin E. Eades, Bailey A. Bye, Mariana T. Ruckert, Joseph Ambrose, Michael N. VanSaun. Investigating a non-canonical role for SHP2 in pancreatic ductal adenocarcinoma metabolism and response to dietary modifications [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2024; Part 1 (Regular Abstracts); 2024 Apr 5-10; San Diego, CA. Philadelphia (PA): AACR; Cancer Res 2024;84(6_Suppl):Abstract nr 4460.
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Cronin, Stephen B. "(Invited) In Situ spectroscopy of Electrochemical and Photoelectrochemical Interfaces". ECS Meeting Abstracts MA2022-02, n.º 48 (9 de outubro de 2022): 1823. http://dx.doi.org/10.1149/ma2022-02481823mtgabs.
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We explore various aspects of electrochemistry and photoelectrochemistry using in situ spectroscopy of electrode (metal) and photoelectrode (semiconductor) interfaces under electrochemical working conditions. Using sum frequency generation (SFG) spectroscopy, we measure the voltage dependence of the orientation of D2O molecules at a graphene electrode surface, which enables us to extract the free energy orienting potential of interfacial water.1 In particular, we measure the “free OD” feature in the spectra, which corresponds to the topmost water molecule that is rotated up out of the bulk water solution and is, therefore, not hydrogen bonded. Using transient absorption spectroscopy (TAS), we measure the lifetime of hot electrons photoexcited in plasmon resonant nanostructures.5 In a similar study, we use transient reflectance spectroscopy (TRS) to measure the photoexcited carrier dynamics in a GaP/TiO2 photoelectrode, as well as the electrostatic field dynamics at this semiconductor-liquid interface in situ under various electrochemical potentials.2 Here, the electrostatic fields at the surface of the semiconductor are measured via Franz−Keldysh oscillations (FKO). These spectra reveal that the nanoscale TiO2 protection layer enhances the built-in field and charge separation performance of GaP photoelectrodes. Using surface enhanced Raman scattering (SERS) spectroscopy, we monitor local electric fields via Stark-shifts of nitrile-functionalized silicon photoelectrodes.6 By monitoring Stark shifts in graphene-enhanced Raman spectroscopy (GERS), we measure local electric fields and local charge densities at monolayer graphene electrode surfaces.3 Lastly, we measure the stacking dependence of monolayer WSe2/MoSe2 heterostructures and observe resonant excitation of interlayer excitons for photocatalytic energy conversion.4 Montenegro, A., C. Dutta, M. Mammetkuliev, H.T. Shi, B.Y. Hou, D. Bhattacharyya, B.F. Zhao, S.B. Cronin and A.V. Benderskii, Asymmetric response of interfacial water to applied electric fields. Nature, 594, 62 (2021). Xu, Z.H., B.Y. Hou, F.Y. Zhao, Z. Cai, H.T. Shi, Y.W. Liu, C.L. Hill, D.G. Musaev, M. Mecklenburg, S.B. Cronin and T.Q. Lian, Nanoscale TiO2 Protection Layer Enhances the Built-In Field and Charge Separation Performance of GaP Photoelectrodes. Nano Letters, 21, 8017-8024 (2021). Shi, H.T., B.F. Zhao, J. Ma, M.J. Bronson, Z. Cai, J.H. Chen, Y. Wang, M. Cronin, L. Jensen and S.B. Cronin, Measuring Local Electric Fields and Local Charge Densities at Electrode Surfaces Using Graphene-Enhanced Raman Spectroscopy (GERS)-Based Stark-Shifts. ACS Applied Materials & Interfaces, 11, 36252-36258 (2019). Chen, J., C.S. Bailey, D. Cui, Y. Wang, B. Wang, H. Shi, Z. Cai, E. Pop, C. Zhou and S.B. Cronin, Stacking Independence and Resonant Interlayer Excitation of Monolayer WSe2/MoSe2 Heterostructures for Photocatalytic Energy Conversion. ACS Applied Nano Materials, DOI:10.1021/acsanm.9b01898 (2020). Yu Wang, Yi Wang, Indu Aravind, Zhi Cai, Lang Shen, Boxin Zhang, Bo Wang, Jihan Chen, Bofan Zhao, Haotian Shi, Jahan M. Dawlaty, and Stephen B. Cronin. In Situ Investigation of Ultrafast Dynamics of Hot Electron-Driven Photocatalysis in Plasmon-Resonant Grating Structures. Journal of the American Chemical Society. DOI: 10.1021/jacs.1c12069 (2022). Haotian Shi, Ryan T. Pekarek, Ran Chen, Boxin Zhang, Yu Wang, Indu Aravind, Zhi Cai, Lasse Jensen, Nathan R. Neale, and Stephen B. Cronin. Monitoring Local Electric Fields using Stark Shifts on Napthyl Nitrile-Functionalized Silicon Photoelectrodes. The Journal of Physical Chemistry C, 124, 17000-17005 (2020).
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Turner, Patrick, e Efren Miranda Zepeda. "Welcoming Ain’t Belonging: A Case Study that Explores How Two-year Predominantly White Colleges Can Foster an Environment of Validation and Mattering for Men of Color". Higher Education Studies 11, n.º 2 (28 de março de 2021): 127. http://dx.doi.org/10.5539/hes.v11n2p127.
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The qualitative case study explored the factors that foster an atmosphere of belonging for men of color (MOC) attending a 2-year Predominantly White Institution. The pressing issue is the PWI colleges make the erroneous assumption that an extension or invitation of welcome is the same as fostering a sense of belonging for men of color (MOC). This led colleges to construct policies and practices that do not intentionally and deliberately create an atmosphere where MOC feel valued, validated, and visible. According to Maestas, Vaquera, and Zehr (2007), fostering a sense of belonging is paramount to the retention and graduation of students of color. The study surfaced three central themes: (a) experiencing an atmosphere of welcome, (b) desire for cultural representation and celebration (c) importance of Diversity, Equity and Inclusion (DEI) training. Though most colleges boast of creating a sense of belonging, the National Center for Education Statistics (2019) reports 25% of men of color graduate from a community college within 150 % or three-years of normal time. Additionally, the Community College Survey of Men (CCSM) reports a lack of validation, engagement, and high attrition rate for men of color attending these institutions (Harris & Wood, 2013). This can be problematic considering the majority of men of color began their academic journey by attending 2-year colleges. Community and 2-year colleges are critical to the educational system and positioned to improve access and equity for students of color, particularly males (Bailey, Jaggars, & Jenkins, 2015).
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Bykova, Y. S., e L. V. Stepanova. "Influence of the process of damage accumulation on the asymptotic behavior of stress fields under creep conditions of a sample with a central crack". Vestnik of Samara University. Natural Science Series 29, n.º 4 (26 de dezembro de 2023): 7–25. http://dx.doi.org/10.18287/2541-7525-2023-29-4-7-25.
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The present study describes the influence of the mutual effect of damage accumulation on the stress fields at the central crack tip in the plate subjected to uniaxial tension. The objective of the study is to analyze the cracked plate experiencing uniaxial loading under creep conditions using the CAE software SIMULIA ABAQUS taking into account the damage accumulation processes near the crack tip. Computations were performed by means of the user procedure UMAT (User material), which is based on the Bailey-Norton creep power law and the Kachanov — Rabotnov damage evolution equation describing the power-law damage accumulation processes. The analysis of the obtained results showed that in the creep and elasticity zones, in computations without taking into account the damage effect, there are asymptotics of stress fields that correspond to well-known analytical solutions of fracture mechanics (Hutchinson — Rice — Rosengren asymptotics for the creep zone, the asymptotics of linear fracture mechanics corresponding to the stress dependence. inversely proportional to the square root of the distance from the tip of the crack – for the zone of elastic behavior of the material. The presence of damage in the cracked specimen affected the asymptotic behavior of the crack tip fields. It is felt that the proposed procedure could pave the way for the analytical solution of the boundary value problem and allow us to determine the structure of the asymptotic solution of the problem. The finite element analysis clearly shows that the process of damage accumulation affects the change in the asymptotic behavior of the stress field in the vicinity of the crack tip and leads to a new asymptotic distribution of the stress tensor components. The proposed procedure can pave the way to an analytical solution of the boundary value problem and allow us to determine the structure of the asymptotic solution of the problem.
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Palazón, Eloy V. "HOW CAN BODIES PERFORM THE FINANCIAL CRISIS? THE FINANCIALIZED BODIES IN CRISTAL PYTE AND SHARON EYAL’S CHOREOGRAPHIES". Acotaciones. Revista de Investigación y Creación Teatral 2, n.º 43 (10 de dezembro de 2019): 75–100. http://dx.doi.org/10.32621/acotaciones.2019.43.03.
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What is the relationship between the economy and dance? How are moving bodies affected by the economic crisis? Is there a cho-reoeconomic condition? If one of the main goals of dance and perfor-mance art during the 20th and 21st centuries has been to understand the relationship between politics and movement, this paper aims at analyzing how financial economy and its crisis can affect the dancing body, and the way dance has the ability to offer alternatives to the finan-cialization of the social body.
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Cronin, Stephen B. "(Invited) In Situ Spectroscopy of Electrocatalytic and Photocatalytic Interfaces". ECS Meeting Abstracts MA2023-01, n.º 46 (28 de agosto de 2023): 2505. http://dx.doi.org/10.1149/ma2023-01462505mtgabs.
Texto completo da fonteResumo:
We report various aspects of electrochemistry and photoelectrochemistry using in situ spectroscopy of electrode (metal) and photoelectrode (semiconductor) interfaces in situ under electrochemical working conditions. These spectroscopies include sum frequency generation (SFG), transient reflectance/absorption spectroscopy (TAS/TRS), and surface enhanced Raman spectroscopy (SERS). Using surface enhanced Raman scattering (SERS) spectroscopy, we monitor local electric fields using Stark-shifts of nitrile-functionalized silicon photoelectrodes.6 Using Graphene-enhanced Raman spectroscopy (GERS)-based Stark-shifts, we measure local electric fields and local charge densities at monolayer graphene electrode surfaces.1 We also measured the stacking dependence and Resonant interlayer excitation of monolayer WSe2/MoSe2 heterostructures for photocatalytic energy conversion.2 Using sum frequency generation (SFG) spectroscopy, we measure the voltage dependence of the orientation of D2O molecules at a graphene electrode surface, which is related back to the “stiffness of the ensemble”.3 In particular, we measured the “free OD” feature in the spectra, which corresponds to the topmost water molecule that is rotated up out of the bulk water solution and is, therefore, not hydrogen bonded. Using transient absorption spectroscopy (TAS), we measure the lifetime of hot electrons photoexcited in plasmon resonant nanostructures.5 Using transient reflectance spectroscopy (TRS), we measure the photoexcited carrier dynamics in a GaP/TiO2 photoelectrode, as well as the electrostatic field dynamics at this semiconductor-liquid interfaces in situ under various electrochemical potentials.4 Here, the electrostatic fields at the surface of the semiconductor are measured via Franz−Keldysh oscillations (FKO). These spectra reveal that the nanoscale TiO2 protection layer enhances the built-in field and charge separation performance of GaP photoelectrodes. Shi, H.T., B.F. Zhao, J. Ma, M.J. Bronson, Z. Cai, J.H. Chen, Y. Wang, M. Cronin, L. Jensen and S.B. Cronin, Measuring Local Electric Fields and Local Charge Densities at Electrode Surfaces Using Graphene-Enhanced Raman Spectroscopy (GERS)-Based Stark-Shifts. ACS Applied Materials & Interfaces, 11, 36252-36258 (2019). Chen, J., C.S. Bailey, D. Cui, Y. Wang, B. Wang, H. Shi, Z. Cai, E. Pop, C. Zhou and S.B. Cronin, Stacking Independence and Resonant Interlayer Excitation of Monolayer WSe2/MoSe2 Heterostructures for Photocatalytic Energy Conversion. ACS Applied Nano Materials, DOI:10.1021/acsanm.9b01898 (2020). Montenegro, A., C. Dutta, M. Mammetkuliev, H.T. Shi, B.Y. Hou, D. Bhattacharyya, B.F. Zhao, S.B. Cronin and A.V. Benderskii, Asymmetric response of interfacial water to applied electric fields. Nature, 594, 62 (2021). Xu, Z.H., B.Y. Hou, F.Y. Zhao, Z. Cai, H.T. Shi, Y.W. Liu, C.L. Hill, D.G. Musaev, M. Mecklenburg, S.B. Cronin and T.Q. Lian, Nanoscale TiO2 Protection Layer Enhances the Built-In Field and Charge Separation Performance of GaP Photoelectrodes. Nano Letters, 21, 8017-8024 (2021). Yu Wang, Yi Wang, Indu Aravind, Zhi Cai, Lang Shen, Boxin Zhang, Bo Wang, Jihan Chen, Bofan Zhao, Haotian Shi, Jahan M. Dawlaty, and Stephen B. Cronin. In Situ Investigation of Ultrafast Dynamics of Hot Electron-Driven Photocatalysis in Plasmon-Resonant Grating Structures. Journal of the American Chemical Society. DOI: 10.1021/jacs.1c12069 (2022). Haotian Shi, Ryan T. Pekarek, Ran Chen, Boxin Zhang, Yu Wang, Indu Aravind, Zhi Cai, Lasse Jensen, Nathan R. Neale, and Stephen B. Cronin. Monitoring Local Electric Fields using Stark Shifts on Napthyl Nitrile-Functionalized Silicon Photoelectrodes. The Journal of Physical Chemistry C, 124, 17000-17005 (2020).
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Wang, X. X., B. Wang, J. L. Liu, J. Chen, X. P. Cui, H. Jiang e D. X. Peng. "First Report of Anthracnose Caused by Colletotrichum gloeosporioides on Ramie in China". Plant Disease 94, n.º 12 (dezembro de 2010): 1508. http://dx.doi.org/10.1094/pdis-08-10-0556.
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Ramie (Boehmeria nivea), usually called “China grass”, is a perennial herbaceous plant belonging to the family Urticaceae with recognized importance in the production of fibers. It is mainly planted in China and other Asian countries including the Philippines, India, South Korea, and Thailand. From June 2007 to September 2010, typical anthracnose symptoms were observed in cultivated ramie fields in HuBei, HuNan, JiangXi, and SiChuan provinces, China, with the diseased area estimated to be more than 10,000 ha. Ramie yield was reduced by 20% on average with up to 55% yield losses in some fields. Lesions were initially small, scattered, round, and gray with brown margin on leaves. As the disease progressed, irregular spots developed and expanded until the leaves withered. Initial lesions on stems were fusiform and expanded, causing the stem to break. Finally, the fibers ruptured. Five isolates (CS-1, CS-2, CS-3, CS-4, and CS-5) were used to evaluate cultural and morphological characteristics of the pathogen. On potato dextrose agar, all isolations initially developed white colonies with orange conidial mass and the colonies turned to gray or brown after 5 days of incubation. Twenty conidia and fifteen setae were measured. Conidia were single celled, colorless, straight, oval, obtuse at both ends, and 11 to 18 × 3 to 6 μm with an average of 14.89 × 4.32 μm. Conidiophores were dense and 11 to 22 × 4 to 5 μm with an average of 15.82 × 4.43 μm. Setae were few, dark brown, one to two septa, and 62 to 71 × 4 to 5 μm with an average of 65.13 × 4.46 μm. The pathogen was identified as Colletotrichum gloeosporioides on the basis of descriptions in Bailey and Jeger (1). Genomic DNA was extracted from the five isolates and sequences of rDNA-ITS with primers ITS1 and ITS4 were obtained (GenBank Accession Nos. GQ120479–GQ120483). Comparison with sequences in GenBank showed 99 to 100% similarity with C. gloeosporioides (Accession Nos. FJ515005, FJ459930, and HM016798). Pathogenicity tests were performed with the five isolates in the laboratory by spraying conidial suspensions (1 × 106 conidia/ml) onto upper and lower surfaces of 10 leaves of 10-day-old, 30-cm high plants. There were three replicate plants for each isolate. The inoculated plants were incubated with a 12-h photoperiod at 25 to 28°C and 90% relative humidity in an artificial climate chamber. Three days after inoculation, brown spots were observed on all inoculated leaves, but no symptoms were seen on water-treated control plants. Koch's postulates were fulfilled by reisolation of C. gloeosporioides from diseased leaves. Though in the revision of Colletotrichum by von Arx (4) and Sutton (3), C. boehmeriae, named based on host specificity, was cancelled, C. boehmeriae was regarded as a pathogen of ramie by some Chinese researchers (2). To our knowledge, this is the first report of C. gloeosporioides causing anthracnose of ramie in China. References: (1) J. A. Bailey and M. J. Jeger. Colletotrichum: Biology, Pathology and Control. CAB International, Wallingford, UK, 1992. (2) R. M. Li and H. G. Ma. J. Plant Prot. 20:83, 1993. (3) B. C. Sutton. Page 523 in: The Coelomycetes: Fungi Imperfecti with Pycnidia, Acervuli and Stromata. Commonwealth Mycological Institute, London, 1980. (4) J. A. von Arx. Phytopathol. Z. 29:413, 1957.
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Maghiar, Diana, Nicoleta Anamaria Pascalau, Florian Maghiar e Liviu Lazar. "Can CRP and ESR values may be influenced by rehabilitation therapy in patients with psoriatic arthritis?" Romanian Journal of Rheumatology 31, n.º 2 (30 de junho de 2022): 66–69. http://dx.doi.org/10.37897/rjr.2022.2.3.
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Objective. Psoriatic arthritis (PsA) is a type of chronic inflammatory arthritis that is linked to psoriasis and affects 20 to 30 percent of those who have it. Clinical symptoms vary and can change over time as one articular pattern evolves into another. This condition has a significant financial and psychological cost attached to it, not to mention the mental state of the patient in relation to the disease. Inflammatory markers such as C-reactive protein (CRP) and erythrocyte sedimentation rate (ESR) are frequently used in primary care to diagnose and monitor inflammatory illnesses such as infections, autoimmune diseases, and malignancies. Our main goal was to see whether or not there was a difference between inflammatory markers in patients with psoriatic arthritis who benefited balneotherapy and kinesiotherapy, compared to those who remained only on the fund treatment. Material and methods. We performed a comparative retrospective study regarding the evolution of 110 patients diagnosed with psoriatic arthritis who underwent or not a rehabilitation program that was performed in Rehabilitation Hospital Baile Felix. Outcomes. One of the results we wanted to demonstrate was whether this type of treatment influences the values of CRP and ESR, thus proving the benefits or disadvantages of this treatment. Conclusion. To answer the major question, rehabilitation therapy DOES influence the values of CRP and ESR values in patients with psoriatic arthritis in the sense of decreasing their values. The 2 week-long rehabilitation therapy was capable of reducing the inflammatory process, a conclusion indicated by the decreasing of CRP and ESR values (mean value difference p <0.001).
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Austin, Jennifer L., e Jason A. Marshall. "BRIDGING THE MARKETING GAP: A REVIEW OF HOW TO THINK LIKE A BEHAVIOR ANALYST: UNDERSTANDING THE SCIENCE THAT CAN CHANGE YOUR LIFE BY JON BAILEY AND MARY BURCH". Journal of Applied Behavior Analysis 41, n.º 1 (março de 2008): 149–54. http://dx.doi.org/10.1901/jaba.2008.41-149.
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Swinburne, T. R. "Colletotrichum: Biology, Pathology and Control, eds J. A. Bailey & M. J. Jeger. xii + 388 pp. Wallingford: CAB International (1992). £60.00 or $114.00 (hardback). ISBN 0 85198 756 7." Journal of Agricultural Science 121, n.º 1 (agosto de 1993): 136–37. http://dx.doi.org/10.1017/s0021859600076929.
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Schwarczinger, I., L. Vajna e W. L. Bruckart. "First Report of Colletotrichum gloeosporioides on Russian-thistle". Plant Disease 82, n.º 12 (dezembro de 1998): 1405. http://dx.doi.org/10.1094/pdis.1998.82.12.1405b.
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A pathogen identified as Colletotrichum gloeosporioides (Penz.) Penz. & Sacc. in Penz. was isolated from foliar and stem lesions on Russian-thistle (Salsola tragus Torner ex L.) collected in Bugac, Hungary, in 1996. Symptoms on leaves and stems began as discrete, sunken, 2- to 10-mm-diameter chlorotic spots, followed by formation of circular buff-colored lesions that eventually coalesced, desiccated, and caused plant tissue death above the lesions. Lesions that occurred near ground level usually killed the plant. Salmon-colored spore masses developed in setose acervuli in the center of the necrotic lesions. Conidia were hyaline, one-celled, falcate to nearly straight, and measured 15 to 25 × 5 to 6 μm. The teleomorph stage of the pathogen (Glomerella cingulata (Stoneman) Spauld. & H. Schrenk) was not observed in the field or on inoculated plants. These morphological characteristics of the isolate were consistent with the description of C. gloeosporioides (1). Pathogenicity was proved by completing Koch's postulates in Hungary and the U.S. Inoculation with conidial suspension (106 conidia per ml) sprayed on S. tragus plants in the greenhouse at the three- to four-leaf stage caused severe necrosis and wilting within 6 days and plant death in 2 weeks. Symptoms did not appear on control plants inoculated with sterile, distilled water. Inoculation test was repeated on 6-week-old plants and at the stage of flowering. All treated plants were killed at both stages within 4 weeks. Because of high virulence and host specificity of this isolate of C. gloeosporioides in preliminary pathogenicity tests it is being evaluated for use as a mycoherbicide for Russian-thistle control in the U.S. This is the first report of C. gloeosporioides causing anthracnose on S. tragus. Reference: (1) B. C. Sutton. Pages 1–27 in: Colletotrichum Biology, Pathology and Control. J. A. Bailei and M. J. Jeger, eds. CAB Int., Wallingford, UK, 1992.
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Tosi, L., R. Buonaurio e C. Cappelli. "Occurrence of Anthracnose Caused by Colletotrichum malvarum on Althaea officinalis in Italy". Plant Disease 88, n.º 4 (abril de 2004): 425. http://dx.doi.org/10.1094/pdis.2004.88.4.425b.
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The cultivation of medicinal plants is increasing in some areas of central Italy where the climate is suitable for organic farming and the production of high-quality plant products. During April and May 2003, plants of Althaea officinalis L. at the seedling stage (two-to-four true leaves) maintained in unheated greenhouses before their transplantation to open fields showed an unusual foliar disease. Necrotic leaf spots of variable shape and size were followed by a rapid wilting of leaves that frequently resulted in a blight of the young plants. Small leaf pieces showing symptoms were sampled, surface treated in 0.1% HgCl2 for 30 s, rinsed twice in sterile water, placed on potato dextrose agar (PDA) (pH 5.5) in petri dishes, and incubated for 7 days at 25 ± 2°C. Colletotrichum malvarum (Braun & Casp.) Southworth (1,2) was consistently recovered from affected tissues. The fungus produced dark colonies with whitish aerial mycelium and acervuli containing hyaline, cylindrical conidia (14 to 25 × 3 to 6 μm) on PDA. The pathogenicity of four fungal isolates was tested by inoculating two, true leaves of 10 plants (A. officinalis) with a conidial suspension (5 × 105 conidia ml-1) from a 10-day-old culture. Plants sprayed with water served as controls. All seedlings were placed in a greenhouse at 24± 2°C under natural light conditions and covered with plastic bags for the first 24 h. Each pathogenicity test was repeated one time. After 5 to 7 days, the inoculated seedlings showed small necrotic leaf spots identical to those observed under natural conditions. Affected leaf areas rapidly enlarged and within a few days, the young plants wilted. No symptoms appeared on the noninoculated controls. C. malvarum was consistently reisolated from the symptomatic test seedlings, whereas the fungus was never isolated from control plants. Standard seed health methods (agar plate and blotter) carried out on samples from the same seed lots used for the unheated greenhouse trials were negative for the presence of the pathogen. The occurrence of anthracnose may be attributed to windborne conidia of C. malvarum coming from infected wild malvaceae species and cultivated hosts grown in open fields in the neighborhood of seedling greenhouses. To our knowledge, this is the first report of C. malvarum on A. officinalis in Italy. References: (1) W. Brandenburger. Page 386 in: Parasitische pilze an gefäbpflanzen in Europa. Fisher Verlag, Stuttgart, Germany, 1985. (2) B. C. Sutton. The genus Glomerella and its anamoroph Colletotrichum. Pages 1–26 in: Colletotrichum, Biology, Pathology and Control. J. A. Bailey and M. J. Jeger eds. CAB International, Wallingford, U.K., 1992.
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Ly, Thuc, Bailey Pickard, Avisha Pandey, Noraida Martinez-Rivera, Eduardo Rosa-Molinar, Michael Washburn, Wen-Xing Ding e Sufi Mary Thomas. "Abstract 6803: TRIM16 regulates IL-6 secretion in head and neck cancer-associated fibroblasts". Cancer Research 84, n.º 6_Supplement (22 de março de 2024): 6803. http://dx.doi.org/10.1158/1538-7445.am2024-6803.
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Abstract Despite current therapy, head and neck squamous cell carcinoma (HNSCC), the 7th most common cancer worldwide, is associated with high morbidity and mortality. Response to current therapies could be improved with a better understanding of the tumor microenvironment of HNSCC. We previously reported that cancer-associated fibroblasts (CAFs), the most abundant stromal cell type in the HNSCC microenvironment, have elevated levels of basal autophagy than oral fibroblasts from cancer-free subjects (NF). Disrupting autophagy in CAFs with Beclin-1 siRNA reduced secretion of IL-6, IL-8, and other factors known to promote HNSCC progression. The recent emerging role of autophagy in secretion of tumor promoting factors has high impact because (i) the mechanism can provide a new target to block the secretion of protumor factors from CAFs that contribute to HNSCC progression, and (ii) these cytokines can serve as potential indicators for the efficacy of autophagy-targeted therapies. To this end, we carried out an unbiased assessment of the proteins associated with autophagosomes in primary CAFs from HNSCC samples using mass spectrometry. We performed immunoprecipitation of LC3B from the isolated small vesicles of the CAFs, identified and validated the association of several trafficking proteins in secretory autophagy including VAMP3 and SNAP23. Further, the tripartite motif (TRIM) proteins have been shown to mediate the fate of autophagosomes either for degradation or secretion. We hypothesized that TRIM proteins are involved in transport of secretory autophagosomes. Our data demonstrate CAFs express higher levels of TRIM16 mRNA than NF. We demonstrate immunogold labeling of TRIM16 or IL-6 localized to the autophagosomes using transmission electron microscopy. Further, using immunofluorescence, we demonstrated the colocalization of TRIM16 and IL-6, as well as IL-6 and LC3B in cytoplasmic puncta. In addition, we used proximity ligation assays to confirm the interaction of TRIM16 and IL-6, as well as IL-6 and LC3B. TRIM16 knockdown using siRNA in CAFs reduced the secretion of IL-6 as measured by ELISA. In conclusion, we demonstrate an important role of TRIM16 in secretory autophagy making it a potential therapeutic target to mitigate CAF-mediated HNSCC growth. Citation Format: Thuc Ly, Bailey Pickard, Avisha Pandey, Noraida Martinez-Rivera, Eduardo Rosa-Molinar, Michael Washburn, Wen-Xing Ding, Sufi Mary Thomas. TRIM16 regulates IL-6 secretion in head and neck cancer-associated fibroblasts [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2024; Part 1 (Regular Abstracts); 2024 Apr 5-10; San Diego, CA. Philadelphia (PA): AACR; Cancer Res 2024;84(6_Suppl):Abstract nr 6803.
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Ireland, K. B., N. A. Haji Mohamad Noor, E. A. B. Aitken, S. Schmidt e J. C. Volin. "First Report of Glomerella cingulata (Colletotrichum gloeosporioides) Causing Anthracnose and Tip Dieback of Lygodium microphyllum and L. japonicum in Australia". Plant Disease 92, n.º 9 (setembro de 2008): 1369. http://dx.doi.org/10.1094/pdis-92-9-1369a.
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The Old World climbing fern, Lygodium microphyllum (Cav.) R. Br., and Japanese climbing fern, L. japonicum (Thunb.) Sw., are invasive noxious weeds in Florida (1). Exploratory surveys for classical biological control agents of L. microphyllum in the fern's native range of Australia and Asia have focused on aboveground herbivores (1). From February to August 2006, fungi were isolated from symptomatic foliage, including lesions associated with leaf curls caused by the mite Flocarus perrepae Knihinicki & Boczek., obtained from L. microphyllum at sites across southeast Queensland, Australia and from both fern species grown at the CSIRO Long Pocket Laboratories in Brisbane, Australia. Anthracnose symptoms with chlorotic margins, initiating at the tip or base of the individual pinnules, were observed on fronds. Dieback symptoms affected growing tips, with sunken lesions and a gradual necrotic wilt as far as the next growth junction of pinnae. Sections from diseased margins were surface sterilized, placed onto water agar, and incubated at 23°C with a 16-h photoperiod. Variable colonies of white-to-gray mycelia, felted or tufted with complete margins, grew well on oatmeal agar and potato dextrose agar. Conidia were hyaline to light salmon, aseptate, straight, and cylindrical (10.4 to 18.2 × 2.6 to 5.2 μm), borne in salmon-to-bright orange masses at 25°C, and consistent with previous descriptions of Colletotrichum gloeosporioides (Penz.) Penz. & Sacc. (3), anamorph of Glomerella cingulata (2). Asci that formed after 3 to 4 weeks in culture were eight-spored, clavate to cylindrical (46.8 to 62.4 × 9.1 to 11.7 μm), and thickened at the apex, and ascospores were cylindrical (11.7 to 18.2 × 3.9 to 5.2 μm), slightly curved, unicellular and hyaline, which is consistent with descriptions of G. cingulata (2). No fruiting bodies were observed in planta; acervuli, setae, and perethecia were not observed. Identification was further confirmed by molecular analysis using the primer pair ITS1/ITS4 (4) (GenBank Accession No. EU697014), indicating 100% similarity to isolates of G. cingulata. To confirm pathogenicity, Koch's postulates were performed on three plants of L. japonicum and 12 plants of L. microphyllum, with an equal number of controls. Conidial suspensions were made to 1.7 × 106 conidia ml–1. During the experiments in the glasshouse, temperatures ranged from 12.6 to 40°C and relative humidity from 39 to 85%. Tips and fronds were collected after 2 to 8 weeks and isolation and identification performed. G. cingulata was consistently reisolated from diseased tissue. No symptoms appeared on controls and isolations did not yield the pathogen. To our knowledge, this is the first report of G. cingulata infecting L. microphyllum and L. japonicum in Australia. Its potential as a biological control agent in the ferns' introduced range remains to be tested. References: (1) J. A. Goolsby et al. Biol. Control. 28:33, 2003. (2) J. E. M. Mordue. Glomerella cingulata. No. 315 in: CMI Descriptions of Pathogenic Fungi and Bacteria. CAB, Kew, UK, 1971. (3) B. C. Sutton. The Genus Glomerella and its Anamorph Colletotrichum. In: Colletotrichum: Biology, Pathology and Control. J. A. Bailey and M. J. Jeger, eds. CAB International, Wallingford, UK, 1992. (4) T. M. White et al. Amplification and Direct Sequencing of Fungal Ribosomal RNA for Phylogenetics. Page 315 in: PCR Protocols: A Guide to Methods and Applications. M. A. Innis et al., eds. Academic Press, San Diego, 1990.
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Pallerla, Aaditya, Bailey Conrad, Amna Bibi, Nyelia Williams, Caroline Wheeler, Rebecca Hoyd, Shankar Suman et al. "Abstract 4435: A causal modeling platform for testing lifestyle interventions on the microbiome and response to immunotherapy". Cancer Research 83, n.º 7_Supplement (4 de abril de 2023): 4435. http://dx.doi.org/10.1158/1538-7445.am2023-4435.
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Abstract Rational manipulation of the gut microbiome by diet or other interventions is a promising approach to improving the efficacy and safety of cancer immunotherapy. However, linking lifestyle variables to specific microbial populations and the host immune response to cancer therapies is challenging due to their complexity. We established an experimental system to examine such complexity by linking a human dietary intervention and fecal biospecimens to mouse models to test the response to immunotherapy. Longitudinal human microbiome samples collected before and after a specific dietary or other intervention allow us to test the hypothesis that an intervention alters the response to immunotherapy via impact on the host microbiome. One set of samples was taken from the BEWELL study, which examined the impact of 2 × 80 mL black raspberry (BRB) drink boxes per day for 4 weeks in people at high risk for lung cancer. Participant samples were chosen based on enrichment of specific taxa. Pre- and post-BRB intervention samples were gavaged into mice. Mouse mc38 cells were injected subcutaneously and treated with anti-PD1 Ab or isotype control. Tumor size was monitored, and at the end of the study, tumor immune cell composition data were collected. Tumor growth over time was modeled using a linear mixed-effects model with tumor volume as the outcome variable and the predictor variables of time and treatment (PD1 vs. IgG) interacting with gavage and quadratic time to accommodate non-linear tumor growth and including random effects by mouse. For sample 68, enriched for Roseburia CAG 309, the interaction between time, gavage post-BRB, and Anti-PD1 treatment significantly affected tumor volume (p < 0.05) relative control. The same significant effect (p < 0.05) was also seen for sample 79, enriched for Lachnospira pectinoschiza, and samples 84 and 85, enriched for Blautia obeum. To determine the mechanism by which this might occur, we analyzed the tumor's immune cell composition. Post-BRB mice treated with Anti-PD1 showed a significant increase in tumor-infiltrating CD8+ immune cells relative control, as compared to mice gavaged with pre-BRB stool. Modeling results showed samples whose response was improved slightly after the BRB dietary intervention. These samples were associated with the enrichment of the taxon Roseburia CAG 309. A black-raspberry dietary intervention in humans modified the microbiomes of several participants in a way that is hypothesized to improve response to PD1 treatment. Future directions include supplementing individual microbes into pre-intervention gavages to confirm which taxa improve response. These results suggest that this modeling platform is an effective system for testing microbiome modification on tumor growth and assessing mechanisms by which this might occur and is extendable to other lifestyle-based interventions where pre- and post-intervention specimens are collected. Citation Format: Aaditya Pallerla, Bailey Conrad, Amna Bibi, Nyelia Williams, Caroline Wheeler, Rebecca Hoyd, Shankar Suman, Joseph Amann, Yangyang Liu, Marisa Bittoni, Shiqi Zhang, Madison Grogan, Alvin Anand, Najma Afrah, Carolyn Presley, Fred K. Tabung, Lang Li, Yael Vodovotz, Jiangjiang Zhu, David P. Carbone, Steven K. Clinton, Daniel Spakowicz. A causal modeling platform for testing lifestyle interventions on the microbiome and response to immunotherapy. [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2023; Part 1 (Regular and Invited Abstracts); 2023 Apr 14-19; Orlando, FL. Philadelphia (PA): AACR; Cancer Res 2023;83(7_Suppl):Abstract nr 4435.
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Palmateer, A. J., R. C. Ploetz, E. van Santen e J. C. Correll. "First Occurrence of Anthracnose Caused by Colletotrichum gloeosporioides on Pitahaya". Plant Disease 91, n.º 5 (maio de 2007): 631. http://dx.doi.org/10.1094/pdis-91-5-0631a.
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Pitahaya, Hylocereus undatus Britt. & Rose, is a columnar, climbing cactus that produces a commercially important fruit. In December 2004, a new disease was found on the crop in Miami-Dade County, FL. Reddish brown lesions with conspicuous chlorotic haloes developed concentrically on the edges of vine ribs. Lesion centers became white and coalesced to rot much of the vine column, and in severe cases, only the vascular column in the vine center was not diseased. Salmon-colored spores and waxy, subepidermal acervuli, typically with setae and simple, short, erect conidiophores, were observed in lesion centers. Tissue from lesion margins was surface disinfested and plated on potato dextrose agar (PDA; Difco Laboratories, Detroit, MI). Colletotrichum gloeosporioides (Penz.) Penz. & Sacc. was isolated from all samples. Colonies produced abundant conidia that were hyaline, one celled, straight, cylindrical, and averaged 14.7 × 5.0 μm with ranges of 12.5 to 17.5 × 3.8 to 7.5 μm (1). Cultural and morphological characteristics of isolates matched those for C. gloeosporioides except for appressoria and hyphopodia (1,2); pitahaya isolates had a spherical rather than lobed hyphopodia reported for C. gloeosporioides and averaged 10.9 (8.5 to 12.7) × 9.1 (7.1 to 10.3) μm. Internal transcribed spacer sequences for the pitahaya isolates were nearly identical (98% homology) to those for C. gloeosporioides isolates occurring on Euphatorium thymifolia in Thailand (GenBank Accession No. AY266393). Koch's postulates were examined in greenhouse trials at the Tropical Research and Education Center, Homestead, FL. Treatments consisted of a noninoculated control, four C. gloeosporioides isolates, and an Alternaria sp. All isolates came from symptomatic pitahaya tissue collected in Miami-Dade County. Fungi were grown on PDA for 7 days at 27°C. A sterile dissecting needle was used to gently pinprick the epidermis of the stem and 2-mm-diameter plugs of C. gloeosporioides, an Alternaria sp., or clean PDA were placed over wounds. Plants were placed in a plastic tent in a greenhouse where the temperature was held at 25°C, and free moisture was maintained on plant surfaces with a household humidifier for 48 h following inoculation. Two isolates of C. gloeosporioides were shown, in repeated greenhouse experiments, to cause reddish brown lesions with conspicuous chlorotic haloes that coalesced to rot much of the vine column, and Koch's postulates were completed with the reisolation of isolates that were used to inoculate plants. The age of vine segments had no significant effect on lesion development. To our knowledge, this is the first report of C. gloeosporioides as a pathogen of pitahaya. References: (1) J. A. Bailey and M. J. Jeger. Colletotrichum: Biology, Pathology and Control. CAB International, Wallingford, UK, 1992. (2) M. Du et al. Mycologia 97:641, 2005.
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Wehrli, Marc, Adam Kuo, Rebecca Larson, Irene Scarfò, Amanda Bouffard, Korneel Grauwet, Mark Leick et al. "Abstract 569: Mesothelin CAR T cells secreting FAP specific T cell engaging molecule (TEAM) target pancreatic cancer and its tumor microenvironment (TME)". Cancer Research 82, n.º 12_Supplement (15 de junho de 2022): 569. http://dx.doi.org/10.1158/1538-7445.am2022-569.
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Abstract Targeting solid tumors with CAR T cells has proven to be more difficult due to their heterogenous target antigen expression, antigen escape, and due to their hostile tumor microenvironment (TME). Mesothelin represents a promising surface tumor antigen, since it has been associated with tumor invasion and is highly expressed on various cancer types, including pancreatic adenocarcinoma. As clinical trials of mesothelin targeting CAR have yet to show efficacy, we hypothesized that tumor stromal cells such as cancer-associated fibroblasts (CAFs) may play a role in this resistance. To provide a more favorable TME for CAR T cells, we generated a bicistronic lentiviral vector encoding a mesothelin CAR along with a secreted T cell engaging molecule (TEAM) that targets fibroblast activation protein (FAP), which is expressed by CAFs. We termed our constructs CARTEAM, and in this case, mesoFAP. We have assessed the activation and proliferative capacity of these CARTEAM through in vitro assays. We showed that TEAMs secreted by CAR T cells bind their appropriate target antigen by adding supernatant from CARTEAM to target cells expressing FAP. In a co-culture assay using a transwell system we demonstrated the cytotoxic effect of secreted TEAM interacting and recruiting bystander T cells against CAFs. In a real-time cell analysis (RTCA) co-culture assay with a pancreatic cancer cell line (AsPC1) and FAP expressing CAFs, we showed cell death of AsPC1 upon CAR recognition and cell death of CAFs through TEAM-mediated recruitment of bystander T cells and CART cells. We show in these co-culture systems, mimicking tumor and TME, that our CARTEAM construct is superior in the elimination of both cancer cell line and CAF, in comparison to control constructs, including mesothelin targeting CAR T cells (meso CAR) and meso CAR T cells secreting an unspecific CD19 TEAM (mesoCD19). We also used acoustic force microscopy to evaluate the additive effect of the TEAM molecule secreted to binding to tumor cells by mesothelin CAR T cells. In vivo experiments of subcutaneously injected tumor cells admixed with CAFs, show superior tumor control when treated with CARTEAM in comparison to control constructs. Based on these data, we demonstrate both the effective in vitro elimination of CAFs and pancreatic cancer cells through the application of CARTEAM and control of pancreatic tumor growth in vivo. Our studies provide a deeper insight into a dual targeting strategy using a novel CAR T cell secreting a TEAM against pancreatic cancer and its tumor microenvironment. Citation Format: Marc Wehrli, Adam Kuo, Rebecca Larson, Irene Scarfò, Amanda Bouffard, Korneel Grauwet, Mark Leick, Andrea Schmidts, Stefanie Bailey, Tamina Kienka, Michael Kann, Sonika Vatsa, Harrison Silva, Kathleen Gallagher, Max Jan, Bryan Choi, David Ting, Marcela Maus. Mesothelin CAR T cells secreting FAP specific T cell engaging molecule (TEAM) target pancreatic cancer and its tumor microenvironment (TME) [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2022; 2022 Apr 8-13. Philadelphia (PA): AACR; Cancer Res 2022;82(12_Suppl):Abstract nr 569.
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Williamson, Brian. "Microbial Ecology of Aerial Plant Surfaces. Edited by M. J. Bailey, A. K. Lilley, T. M. Timms-Wilson and P. T. N. Spencer-Phillips. Wallingford, UK: CAB International (2006), pp. 315, £75.00. ISBN-13: 978-1-84593-061-5". Experimental Agriculture 43, n.º 4 (outubro de 2007): 528. http://dx.doi.org/10.1017/s0014479707005546.
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Park, J. H., e S. T. Seo. "First Report of Anthracnose of Smoothlip Cymbidium (Cymbidium kanran) Caused by Colletotrichum gloeosporioides in Korea". Plant Disease 97, n.º 7 (julho de 2013): 997. http://dx.doi.org/10.1094/pdis-12-12-1167-pdn.
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Smoothlip cymbidium (Cymbidium kanran Makino) is one of the national endangered species in Korea and in situ and ex situ conservation measures have been implemented for its threatened habitat and population. Jeju Island in Korea is one of few places where wild populations of the plant remain. In 2012, leaf blotch and blight occurred on leaves of smoothlip cymbidium in Jeju Island throughout the growing season. Affected plants were collected in May and October to determine the causal agent. Leaves had multiple brownish lesions that often developed from the leaf tip or leaf margin, eventually coalescing together and proceeding toward the base. In the lesions, fungal fruiting bodies formed concentric rings and exuded salmon-colored spore masses when put in moist chambers for 1 to 2 days. Colletotrichum gloeosporioides was consistently isolated from the spore masses or the margins of fresh lesions. When cultured on potato dextrose agar (PDA), colonies were olive to gray on the upper side and dark gray to black on the reverse side. On affected leaves, mature acervuli were dark brown to black, waxy, subepidermal, circular to ellipsoid, and 142 to 255 μm in diameter. Setae on the acervuli were dark brown, acicular, and 67 to 103 μm long. Conidia were hyaline, cylindrical with both ends rounded, and 13.7 to 19.2 × 4.0 to 6.1 μm. Hyphopodia were lobed and 7.6 to 13.7 × 5.7 to 11.6 μm (43 to 157 μm2 in area). These morphological characteristics were consistent with descriptions of C. gloeosporioides (1,3). The identities of two representative isolates were confirmed by sequencing the internal transcribed spacer (ITS) regions and the large subunit (LSU) rDNA (GenBank Accession Nos. KC408373, KC408374, and KC408375). BLAST analysis of the sequences from each isolate against the GenBank database found 99% similarities to more than 30 accessions of C. gloeosporioides (e.g., AY266392, EU552111). Pathogenicity was tested with one isolate on four leaves obtained from four plants of asymptomatic smoothlip cymbidium. Two places on the epidermis of each leaf were gently pinpricked using a sterile dissecting needle. One disk (0.6 cm diameter) of either PDA containing the fungus or sterile water agar was placed on one of the two places on each leaf. Laboratory film and aluminum foil held the disks in place. Inoculated leaves were kept in a moist chamber for 24 hr. After 1 week, buff-colored lesions with young acervuli were found on all fungus inoculation sites but no lesions occurred with control inoculations. C. gloeosporioides was recovered from all inoculations, but not from the controls. To our knowledge, this is the first report of anthracnose caused by C. gloeosporioides on smoothlip cymbidium in Korea. The same fungus has been previously reported to cause anthracnose of other orchid plants in the genera Aspidistra, Cymbidium, and Dendrobium in Korea (2). This pathogen can pose a threat to smoothlip cymbidium. References: (1) M. Du et al. Mycologia 97: 641, 2005. (2) The Korean Society of Plant Pathology. List of Plant Diseases in Korea, 5th ed. The Korean Society of Plant Pathology, Suwon, Korea, 2009. (3) B. C. Sutton. Pages 1-26 in: Colletotrichum: Biology, Pathology, and Control. J. A. Bailey and M. J. Jeger, eds. CAB International, Wallingford, U.K., 1992.
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Kolomiets, T., O. Skatenok, A. Alexandrova, Z. Mukhina, T. Matveeva, D. Bogomaz, D. K. Berner e C. A. Cavin. "First Report of Anthracnose of Salsola tragus Caused by Colletotrichum gloeosporioides in Russia". Plant Disease 92, n.º 9 (setembro de 2008): 1366. http://dx.doi.org/10.1094/pdis-92-9-1366b.
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In October of 2006, dying Salsola tragus L. (Russian thistle, tumbleweed), family Chenopodiaceae, plants were found along the Azov Sea at Chushka, Russia. Approximately 40 plants in the area were diseased and almost 80% of these were dying. Plants were approximately 1 m tall × 0.5 m wide. Dying plants had irregular, necrotic lesions along the length of the stems. Leaves of these plants were also necrotic. Lesions on stems and leaves were dark brown and usually coalesced. Diseased stems were cut into 3- to 5-mm pieces, disinfested in 70% ethyl alcohol, and then placed onto the surface of potato glucose agar (PGA). Numerous, waxy, subepidermal acervuli with 110 μm long (mean) black setae were observed in all of the lesions after 2 to 3 days. Conidiophores were simple, short, and erect. Conidia were one-celled, hyaline, ovoid to oblong, falcate to straight, and measured 12.9 to 18.0 × 2.8 to 5.5 μm (mean 15.6 × 4.2 μm). Appressoria formed 24 h after placing conidia on a dialysis membrane over 20% V8 juice agar. Appressoria measured 4.0 to 13.9 × 2.4 to 8.8 μm (mean 7.0 × 5.2 μm). These characters conformed to the description of Colletotrichum gloeosporioides (Penz.) Penz. & Sacc. in Penz. (1). A voucher specimen was deposited with the U.S. National Fungus Collections, Beltsville, MD (BPI 878389). Nucleotide sequences for the internal transcribed spacers (ITS 1 and 2) were deposited in GenBank (Accession No. EU530697) and aligned with ITS sequences of two other isolates from S. tragus. There was 100% similarity to each isolate, one from Greece (Accession No. DQ344621) and one from Hungary (Accession No. EU805538). Axenic cultures on PGA were sent to the Foreign Disease-Weed Science Research Unit, USDA, ARS, Fort Detrick, MD for testing in quarantine. Conidia were harvested from 14-day-old cultures grown on 20% V8 juice agar, and healthy stems and leaves of 30-day-old plants of S. tragus (13 plants) were spray inoculated with an aqueous conidial suspension of 1.0 × 106 conidia/ml plus 0.1% v/v polysorbate 20. Another 13 control plants were sprayed with water and surfactant without conidia. Plants were placed in an environmental chamber at 100% humidity for 16 h in the dark at 25°C. After approximately 24 h, all plants were transferred to a greenhouse at 20 to 25°C, 30 to 50% relative humidity, and natural light augmented by 12-h light periods with 500 W sodium vapor lights. Lesions developed on stems of all inoculated plants after 7 days. After 14 days, nine plants were dead and all inoculated plants were dead after 3 weeks. No symptoms developed on control plants. C. gloeosporioides was reisolated from stem pieces of all inoculated plants, and the morphology of the reisolated pathogen was the same as that of the initially isolated pathogen. To our knowledge, this is the first report of anthracnose caused by C. gloeosporioides on S. tragus in Russia. Reference: (1) B. C. Sutton. Page 15 in: Colletotrichum Biology, Pathology and Control. J. A. Bailey and M. J. Jeger, eds. CAB International, Wallingford, UK, 1992.
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Park, J. H., K. S. Han, Y. D. Kwon e H. D. Shin. "First Report of Anthracnose of Tricyrtis macropoda Caused by Colletotrichum gloeosporioides in Korea". Plant Disease 96, n.º 7 (julho de 2012): 1070. http://dx.doi.org/10.1094/pdis-03-12-0277-pdn.
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Tricyrtis macropoda Miq. (syn. T. dilatata Nakai), known as speckled toadlily, is a perennial herb native to China, Japan, and Korea. The plant has been highly praised for its beautiful flowers and rare populations in natural habitats. In September 2006, several dozen plants were heavily damaged by leaf spots and blight in cultivated plantings in the city of Pocheon, Korea. The infections with the same symptoms were repeated every year. In July 2011, the same symptoms were found on T. macropoda in the cities of Gapyeong and Osan, Korea. The leaf lesions began as small, water-soaked, pale greenish to grayish spots, which enlarged to form concentric rings and ultimately coalesced. A number of blackish acervuli were formed in the lesions. Acervuli were mostly epiphyllous, circular to ellipsoid, and 40 to 200 μm in diameter. Setae were two- to three-septate, dark brown at the base, paler upwards, acicular, and up to 100 μm long. Conidia (n = 30) were long obclavate to oblong-elliptical, sometimes fusiform-elliptical, guttulate, hyaline, and 12 to 20 × 4 to 6.5 μm (mean 15.4 × 5.2 μm). These morphological characteristics of the fungus were consistent with the description of Colletotrichum gloeosporioides (Penz.) Penz. & Sacc. (2). Voucher specimens (n = 7) were deposited in the Korea University herbarium (KUS). Two isolates, KACC46374 (ex KUS-F25916) and KACC46405 (ex KUS-F26063), were deposited in the Korean Agricultural Culture Collection. Fungal DNA was extracted and the complete internal transcribed spacer (ITS) region of rDNA was amplified with the primers ITS1/ITS4 and sequenced. The resulting sequences of 549 bp were deposited in Genbank (Accession Nos. JQ619480 and JQ619481). They showed 100% similarity with a sequence of C. gloeosporioides (EU32619). Isolate KACC46374 was used in a pathogenicity test. Inoculum was prepared by harvesting conidia from 3-week-old cultures on potato dextrose agar. A conidial suspension (2 × 106 conidia/ml) was sprayed onto 15 leaves of three plants. Three noninoculated plants served as controls. Plants were covered with plastic bags to maintain 100% relative humidity for 24 h and then kept in a greenhouse (22 to 28°C and 70 to 80% RH). After 5 days, typical leaf spot symptoms, identical to the ones observed in the field, started to develop on the leaves of inoculated plants. No symptoms were observed on control plants. C. gloeosporioides was reisolated from the lesions of inoculated plants, thus fulfilling Koch's postulates. An anthracnose associated with C. tricyrtii (Teng) Teng was recorded on T. formosana and T. latifolia in China (3) and on T. formosana in Taiwan (1), respectively, without etiological studies. The morphological features of C. tricyrtii are within the variation of C. gloeosporioides (2). To our knowledge, this is the first report of anthracnose of T. macropoda. This report has significance to indigenous plant resource conservation managers and scientists because T. macropoda has been listed as one of the 126 “Rare and Endangered Plants” by the Korea Forest Service since 1991. References: (1) K. Sawada. Rep. Dept. Agric. Gov. Res. Inst. Formosa 87: 1, 1944. (2) B. C. Sutton. Pages 1–27 in: Colletotrichum Biology, Pathology and Control. J. A. Bailey and M. J. Jeger, eds. CAB International, Wallingford, U.K. 1992. (3) S. C. Teng. Contrib. Biol. Lab. Sci. Soc. China 8:36, 1932.
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Ma, W. J., X. Yang, X. R. Wang, Y. S. Zeng, M. D. Liao, C. J. Chen, S. Sun e D. M. Jia. "First Report of Anthracnose Disease on Young Stems of Bawanghua (Hylocereus undatus) Caused by Colletotrichum gloeosporioides in China". Plant Disease 98, n.º 7 (julho de 2014): 991. http://dx.doi.org/10.1094/pdis-06-13-0609-pdn.
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Hylocereus undatus widely grows in southern China. Some varieties are planted for their fruits, known as dragon fruits or Pitaya, while some varieties for their flowers known as Bawanghua. Fresh or dried flowers of Bawanghua are used as routine Chinese medicinal food. Since 2008, a serious anthracnose disease has led to great losses on Bawanghua flower production farms in the Baiyun district of Guangzhou city in China. Anthracnose symptoms on young stems of Bawanghua are reddish-brown, sunken lesions with pink masses of spores in the center. The lesions expand rapidly in the field or in storage, and may coalesce in the warm and wet environment in spring and summer in Guangzhou. Fewer flowers develop on infected stems than on healthy ones. The fungus overwinters in infected debris in the soil. The disease caused a loss of up to 50% on Bawanghua. Putative pathogenic fungi with whitish-orange colonies were isolated from a small piece of tissue (3 × 3 mm) cut from a lesion margin and cultured on potato dextrose agar in a growth chamber at 25°C, 80% RH. Dark colonies with acervuli bearing pinkish conidial masses formed 14 days later. Single celled conidia were 11 to 18 × 4 to 6 μm. Based on these morphological characteristics, the fungi were identified as Colletotrichum gloeosporioides (Penz.) Penz. & Sacc (2). To confirm this, DNA was extracted from isolate BWH1 and multilocus analyses were completed with DNA sequence data generated from partial ITS region of nrDNA, actin (ACT) and glutamine synthetase (GS) nucleotide sequences by PCR, with C. gloeosporioides specific primers as ITS4 (5′-TCCTCCGCTTATTGATATGC-3′) / CgInt (5′-GGCCTCCCGCCTCCGGGCGG-3′), GS-F (5′-ATGGCCGAGTACATCTGG-3′) / GS-R (5′-GAACCGTCGAAGTTCCAC-3′) and actin-R (5′-ATGTGCAAGGCCGGTTTCGC-3′) / actin-F (5′-TACGAGTCCTTCTGGCCCAT-3′). The sequence alignment results indicated that the obtained partial ITS sequence of 468 bp (GenBank Accession No. KF051997), actin sequence of 282 bp (KF712382), and GS sequence of 1,021 bp (KF719176) are 99%, 96%, and 95% identical to JQ676185.1 for partial ITS, FJ907430 for ACT, and FJ972589 for GS of C. gloeosporioides previously deposited, respectively. For testing its pathogenicity, 20 μl of conidia suspension (1 × 106 conidia/ml) using sterile distilled water (SDW) was inoculated into artificial wounds on six healthy young stems of Bawanghua using sterile fine-syringe needle. Meanwhile, 20 μl of SDW was inoculated on six healthy stems as a control. The inoculated stems were kept at 25°C, about 90% relative humidity. Three independent experiments were carried out. Reddish-brown lesions formed after 10 days, on 100% stems (18 in total) inoculated by C. gloeosporioides, while no lesion formed on any control. The pathogen was successfully re-isolated from the inoculated stem lesions on Bawanghua. Thus, Koch's postulates were fulfilled. Colletotrichum anthracnose has been reported on Pitaya in Japan (3), Malaysia (1) and in Brazil (4). To our knowledge, this is the first report of anthracnose disease caused by C. gloeosporioides on young stems of Bawanghua (H. undatus) in China. References: (1) M. Masyahit et al. Am. J. Appl. Sci. 6:902, 2009. (2) B. C. Sutton. Page 402 in: Colletotrichum Biology, Pathology and Control. J. A. Bailey and M. J. Jeger, eds. CAB International, Wallingford, UK, 1992. (3) S. Taba et al. Jpn. J. Phytopathol. 72:25, 2006. (4) L. M. Takahashi et al. Australas. Plant Dis. Notes 3:96, 2008.
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Berner, D. K., C. A. Cavin, M. B. McMahon e I. Loumbourdis. "First Report of Anthracnose of Salsola tragus Caused by Colletotrichum gloeosporioides in Greece". Plant Disease 90, n.º 7 (julho de 2006): 971. http://dx.doi.org/10.1094/pd-90-0971b.
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In early October of 2005, dying Salsola tragus L. (Russian thistle, tumbleweed), family Chenopodiaceae, plants were found along the Aegean Sea at Kryopigi Beach, Greece (40°02′29″N, 23°29′02″E, elevation 0 m). All of the 30 to 40 plants in the area were diseased and approximately 80% were dead or dying. All plants were relatively large (approximately 1 m tall × 0.5 m diameter), and living portions of diseased plants were flowering. Dying plants had irregular, necrotic lesions extending the length of the stems. Leaves of these plants were also necrotic. Lesions on stems and leaves were dark brown and usually coalesced. Diseased stem pieces were taken to the European Biological Control Laboratory, USDA, ARS at the American Farm School in Thessaloniki, Greece. There, diseased stem pieces were surface disinfested for 15 min with 0.5% NaOCl and placed on moist filter paper in petri dishes. Numerous, waxy subepidermal acervuli with black setae were observed in all lesions after 2 to 3 days. Conidiophores were simple, short, and erect. Conidia were one-celled, hyaline, ovoid to oblong, falcate to straight, 12.9 to 18.0 × 2.8 to 5.5 μm (mode 16.1 × 4.5 μm). These characters conformed to the description of Colletotrichum gloeosporioides (Penz.) Penz. & Sacc. in Penz. (2). Conidia were placed on modified potato carrot agar and axenic cultures from these isolations were sent to the quarantine facility of the Foreign Disease-Weed Science Research Unit, USDA, ARS, Fort Detrick, MD for testing. On the basis of DNA sequences, two variants within S. tragus have been described in California and named “Type A” and “Type B” (1). Conidia were harvested from 14-day-old cultures grown on 20% V8 juice agar, and healthy stems and leaves of 18 30-day-old plants of S. tragus Type A and 10 Type B plants were spray inoculated with an aqueous conidial suspension (1.0 × 106 conidia/ml plus 0.1% non-ionic surfactant). Three control plants of each type were sprayed with water and surfactant only. Plants were placed in an environmental chamber (18 h of dew in darkness at 25°C). After 1 day, all plants were transferred to a greenhouse (20 to 25°C, 30 to 50% relative humidity, and natural light augmented with 12-h light periods with 500-W sodium vapor lights). Lesions developed on stems of inoculated Type A plants after 5 days. After 14 days, all inoculated Type A plants were dead. Lesions on Type B plants were small and localized; all plants were diseased but no plants died. No symptoms occurred on control plants. C. gloeosporioides was reisolated 14 to 21 days after inoculation from stem pieces of all inoculated plants of both types of S. tragus. This isolate of C. gloeosporioides is a destructive pathogen on S. tragus Type A and is a potential candidate for biological control of this weed in the United States. To our knowledge, this is the first report of anthracnose caused by C. gloeosporioides on S. tragus in Greece. A voucher specimen has been deposited with the U.S. National Fungus Collections, Beltsville, MD (BPI 871126). Nucleotide sequences for the internal transcribed spacers (ITS 1 and 2) were deposited in GenBank (Accession No. DQ344621) and exactly matched sequences of the teleomorph, Glomerella cingulata. References: (1) F. Ryan and D. Ayres. Can. J. Bot. 78:59, 2000. (2) B. C. Sutton. Page 15 in: Colletotrichum Biology, Pathology and Control. J. A. Bailey and M. J. Jeger, eds. CAB International Mycological Institute, Wallingford, UK, 1992.
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Crouch, J. A., e J. C. Inguagiato. "First Report of Anthracnose Disease of Ornamental Feather Reed Grass (Calamagrostis × acutifolia ‘Karl Foerster’) Caused by Colletotrichum cereale". Plant Disease 93, n.º 2 (fevereiro de 2009): 203. http://dx.doi.org/10.1094/pdis-93-2-0203a.
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Calamagrostis × acutifolia ‘Karl Foerster’ (feather reed grass) is a cool-season grass grown extensively as an ornamental plant throughout the United States. In July 2005, severe foliar damage was observed in feather reed grasses in a residential garden in Barrington, NJ. Symptoms were observed as small, yellowish brown, oval to irregularly shaped spots on the blades, with spread and coalescence of spots leading to eventual necrosis and plant death. Numerous acervuli with black setae diagnostic of fungi in the genus Colletotrichum were present on necrotic lesions. Two distinct fungi were isolated from diseased tissue by plating small sections of infested leaf tissue on potato dextrose agar (PDA) supplemented with 40 μg/liter each of penicillin, ampicillin, gentomycin, and streptomycin. The first fungus was identified as Colletotrichum gloeosporioides based on morphological, cultural (2), and molecular characteristics. Variable colonies of gray-white mycelia with masses of pink-to-salmon hued conidia formed at 25°C on PDA under constant light. Conidia were hyaline, aseptate, straight, and cylindrical with rounded apices (11.0 to 18.5 × 2.5 to 5.0 μm). Sequencing of the internal transcribed spacer (ITS) rDNA region showed the fungus to be most similar to C. gloeosporioides (GenBank Accession No. EU979125). The second fungus was identified as C. cereale based on morphological, cultural, and molecular characteristics (1). Variable colonies of gray-tan-white mycelia formed at 25°C on PDA under constant light and salmon-colored conidial masses surrounded numerous setae. Conidia were hyaline, aseptate, falcate, fusiform, and guttulate (15.0 to 21.5 × 2.5 to 4.5 μm). Hyphal appressoria were ovoid, sometimes lobate or multilobate (10.5 to 13.5 × 7.5 to 10.0 μm). Maximum likelihood phylogenetic analysis of the ITS sequence (GenBank Accession No. DQ126193) and fungal mating type idiomorph Mat1-2 HMG box (GenBank Accession No. DQ131962) identified the fungus as C. cereale (1). Pathogenicity was determined by inoculating healthy feather reed grasses (11.4-liter pots) established 61 cm off-center in a mulched bed. Three replicate plants per treatment were sprayed with a 20-ml conidial solution (5 × 104 conidia/ml in 0.1 potato dextrose broth) of either C. cereale or C. gloeosporioides and an uninoculated control. Temperatures ranged from 19 to 36°C and humidity varied between 31 and 79%. No symptoms were observed in uninoculated controls or plants inoculated with C. gloeosporioides. Plants inoculated with C. cereale developed disease symptoms within 21 days; the fungus was subsequently reisolated from symptomatic leaves and confirmed as C. cereale. To our knowledge, this is the first report of anthracnose of feather reed grass caused by C. cereale (formerly known as C. graminicola). Although C. cereale is known to inhabit numerous cool-season grass hosts, this is the first description of this fungus as a pathogen of an ornamental grass. Given the recent emergence of anthracnose epidemics caused by C. cereale on golf course turfgrass, the identification of this fungus as a pathogen of Calamagrostis × acutifolia highlights the need for nurseries and regulatory personnel to screen ornamental grasses such as feather reed grass for the presence of C. cereale so that the disease does not become problematic. References: (1) J. A. Crouch et al. Phytopathology 96:46, 2006. (2) B. C. Sutton. The genus Glomerella and its anamorph Colletotrichum in: Colletotrichum: Biology, Pathology and Control. J. A. Bailey and M. J. Jeger, eds. CAB International, Wallingford, UK, 1992.