Literatura científica selecionada sobre o tema "Animaux menacés"

Heavy metals are partitioned into two different groups of essential (non-toxic) and nonessential (toxic) elements. The non-toxic heavy metals are of essential health and nutritional benefits to animals and humans. The clinical consequences of non-essential heavy metals if ingested even in low doses are grave. This study was the concluding part of our previous works on the detection of drug residues and bacterial contaminations of locally processed yoghurt sold for human consumption in Jigawa State of Northern Nigeria. In this present study, we attempted to evaluate the presence of Lead (Pb) and Cobalt (Co) heavy metal residue in samples of locally processed yoghurt hawked for human consumption in selected parts of Jigawa State, Nigeria. About 10 mL each of four samples of locally processed yoghurt samples were randomly collected from each of five collection centers/points in the morning from large containers into sterilized collection tubes and cooled and transported in iced pack box for laboratory analyses. The evaluation of heavy metal residues was performed using high performance liquid chromatography (HPLC)-based assay. We presented from our results that sample of locally processed yoghurt collected from Kiri had the highest Cobalt (Co) residue (75.18 mg/kg) followed by Majiyawa (65.84 mg/kg) both of which were from Ringim dairy collection center while Dakayyawa, Andaza and Balbadu dairy collection centers had Co residue-free yoghurt. Majority of the locally processed yoghurt samples collected from the study area had elevated Pb contamination levels which were far higher than those collected from Rumbawa (0.00 mg/kg), Dakayyawa (0.00 mg/kg), Kyambo (0.02 mg/kg), Dangyati (0.02 mg/kg) and Andaza (0.02 mg/kg) dairy collection centers/points. Our results suggested that Pb contamination levels detected in the study area is clinically unsafe and unhygienic for human consumption and it is of grave consequences to public health. Therefore, urgent action is needed to be taken by appropriate agency of government to address this health threat to the consumers' safety of dairy products in the study area. Les métaux lourds sont répartis en deux groupes différents d'éléments essentiels (non toxiques) et non essentiels (toxiques). Les métaux lourds non toxiques présentent des avantages essentiels pour la santé et la nutrition des animaux et des humains. Les conséquences cliniques des métaux lourds non essentiels en cas d'ingestion même à dosesfaibles sont graves. Cette étude était la conclusion de nos travaux antérieurs sur la détection des résidus de médicaments et des contaminations bactériennes de yaourts transformés localement vendus pour la consommation humaine dans l'État de Jigawa au nord du Nigéria. Dans l'étude actuelle, nous avons tenté d'évaluer la présence de résidus de métaux lourds de plomb (Pb) et de cobalt (Co) dans des échantillons de yaourt transformé localement destiné à la consommation humaine dans certaines régions de l'État de Jigawa, au Nigéria. Environ 10 ml de chacun des quatre échantillons d'échantillons de yaourt transfor és localement ont été prélevés au hasard dans chacun des cinq centres / points de collecte le matin à partir de grands conteneurs dans des tubes de collecte stérilisés et refroidis et transportés dans une boîte d'emballage glacée pour les analyses de laboratoire. L'évaluation des résidus de métaux lourds a été réalisée en utilisant un test basé sur la chromatographie liquide à haute performance (HPLC). Nous avons présenté à partir de nos résultats que l'échantillon de yaourt transformé localement recueilli à Kiri contenait le résidu de cobalt (Co) le plus élevé (75.18 mg / kg) suivi de Majiyawa (65.84 mg / kg), tous deux provenant du centre de collecte laitière de Ringim tandis que Dakayyawa, Andaza et les centr s de collecte des produits laitiers de Balbadu disposaient de yaourt sans résidu de Co. La ma orité des échantillons de yaourt transformés localement prélevés dans la zone d'étude présentaient des niveaux de contamination élevés au plomb qui étaient bien supérieurs à ceux recueillis à Rumbawa (0.00 mg / kg), Dakayyawa (0.00 mg / kg), Kyambo (0.02 mg / kg), Dangyati Centres / points de collecte laitière (0.02 mg / kg) et Andaza (0.02 mg / kg). Nos résultats suggèrent que les niveaux de contamination au plomb détectés dans la zone d'étude sont cliniquement dangereux et insalubres pour la consommation humaine et ont de graves conséquences pour la santé publique. Par conséquent, des mesures urgentes doivent être prises par l'agence gouvernementale appropriée pour faire face à cette menace pour la santé vis-à-vis la sécurité des consommateurs des produits laitiers dans la zone d'étude.

Staphylococcal ocular infections of food animals have been somewhat under diagnosed probably due to the ubiquitous nature of staphylococcal organisms. This study was undertaken to determine the occurrence of staphylococcal ocular infections of food producing animals in Nsukka Southeast, Nigeria, and to determine the antibiogram of the isolated staphylococci. A total of 5,635 food producing animals were externally examined for signs of clinical ocular conditions. Animals that showed clinical eye lesions were further examined using pen light to assess the entire globe and the pupillary reflex. Blindness was assessed using menace blink reflex, palpebral reflex and obstacle methods. Isolation and identification of staphylococcal isolates from ocular swabs were done by standard methods. Antibiogram of the isolates was determined by disc diffusion method. Sixty-three (1.1%) of the examined animals showed signs of ocular condition. Thirty-one (49.2%) of the cultured swabs yieldedStaphylococcus aureus(S. aureus). Isolation rates from different animal species were caprine (60%), ovine (33.3%), bovine (12.5%), and porcine (0%). Resistance of the isolates was 100% to ampicillin/cloxacillin, 90% to tetracycline, 80% to streptomycin, 71% to chloramphenicol, 20% to erythromycin, 16% to gentamicin, and 0% to ciprofloxacin and norfloxacin. Twenty-five (81%) of the isolates were multi-drug resistant. This study has shown that antibiotic-resistant staphylococci are associated with a sizeable percentage of ocular infections of food producing animals and should be considered during diagnosis and treatment.

Diffuse lung injury from hyperoxia is accompanied by low compliance and hypoxemia with disruption of endothelial and alveolar epithelial cell layers. Because both function and content of surfactant in diffuse lung injury decrease in animals and in humans, changes in the extent of injury during continuous hyperoxia were evaluated after treatments with a protein-free surfactant in primates. Ten baboons were ventilated with 100% O2 for 96 h and five were intermittently given an aerosol of an artificial surfactant (Exosurf). Physiological and biochemical measurements of the effects of the surfactant treatment are presented in a companion paper (Y.-C. T. Huang, A. C. Sane, S. G. Simonson, T. A. Fawcett, R. E. Moon, P. J. Fracica, M. G. Menache, C. A. Piantadosi, and S. L. Young. J. Appl. Physiol. 78: 1823–1829, 1995.) After O2 exposures, lungs were fixed and processed for electron microscopy. The cellular responses to O2 included epithelial and endothelial cell injuries, interstitial edema, and inflammation. Morphometry was used to quantitate changes in lungs of animals treated with the artificial surfactant during O2 exposure and to compare them with the untreated animals. The surfactant decreased neutrophil accumulation, increased fibroblast proliferation, and decreased changes in the volume of type I epithelial cells. Surfactant-treated animals also demonstrated better preservation of endothelial cell integrity. These responses indicate ameliorating effects of the surfactant on the pulmonary response to hyperoxia, including protection against epithelial and endothelial cell destruction. Significant interstitial inflammation and fibroblast proliferation remained, however, in surfactant-treated lungs exposed to continuous hyperoxia.

The following are the abstracts of the articles discussed in the subsequent letter: Huang, Yuh-Chin T., Aneysa C. Sane, Steven G. Simonson, Thomas A. Fawcett, Richard E. Moon, Philip J. Fracica, Margaret G. Menache, Claude A. Piantadosi, and Stephen L. Young. Artificial surfactant attenuates hyperoxic lung injury in primates. I. Physiology and biochemistry. J. Appl. Physiol. 78(5): 1816–1822, 1995.—Prolonged exposure to O2causes diffuse alveolar damage and surfactant dysfunction that contribute to the pathophysiology of hyperoxic lung injury. We hypothesized that exogenous surfactant would improve lung function during O2exposure in primates. Sixteen healthy male baboons (10–15 kg) were anesthetized and mechanically ventilated for 96 h. The animals received either 100% O2( n = 6) or 100% O2plus aerosolized artificial surfactant (Exosurf; n = 5). A third group of animals ( n = 5) was ventilated with an inspired fraction of O2of 0.21 to control for the effects of sedation and mechanical ventilation. Hemodynamic parameters were obtained every 12 h, and ventilation-perfusion distribution (V˙A/Q˙) was measured daily using a multiple inert-gas elimination technique. Positive end-expiratory pressure was kept at 2.5 cmH2O and was intermittently raised to 10 cmH2O for 30 min to obtain additional measurements ofV˙A/Q˙. After the experiments, lungs were obtained for biochemical and histological assessment of injury. O2exposures altered hemodynamics, progressively worsenedV˙A/Q˙, altered lung phospholipid composition, and produced severe lung edema. Artificial surfactant therapy significantly increased disaturated phosphatidylcholine in lavage fluid and improved intrapulmonary shunt, arterial Po2, and lung edema. Surfactant also enhanced the shunt-reducing effect of positive end-expiratory pressure. We conclude that an aerosolized protein-free surfactant decreased the progression of pulmonary O2toxicity in baboons.Piantadosi, Claude A., Philip J. Fracica, Francis G. Duhaylongsod, Y.-C. T. Huang, Karen E. Welty-Wolf, James D. Crapo, and Stephen L. Young. Artificial surfactant attenuates hyperoxic lung injury in primates. II. Morphometric analysis. J. Appl. Physiol. 78(5): 1823–1831, 1995.—Diffuse lung injury from hyperoxia is accompanied by low compliance and hypoxemia with disruption of endothelial and alveolar epithelial cell layers. Because both function and content of surfactant in diffuse lung injury decrease in animals and in humans, changes in the extent of injury during continuous hyperoxia were evaluated after treatments with a protein-free surfactant in primates. Ten baboons were ventilated with 100% O2for 96 h and five were intermittently given an aerosol of an artificial surfactant (Exosurf). Physiological and biochemical measurements of the effects of the surfactant treatment are presented in a companion paper (Y.-C. T. Huang, A. C. Sane, S. G. Simonson, T. A. Fawcett, R. E. Moon, P. J. Fracica, M. G. Menache, C. A. Piantadosi, and S. L. Young. J. Appl. Physiol. 78: 1823–1829, 1995.) After O2exposures, lungs were fixed and processed for electron microscopy. The cellular responses to O2included epithelial and endothelial cell injuries, interstitial edema, and inflammation. Morphometry was used to quantitate changes in lungs of animals treated with the artificial surfactant during O2exposure and to compare them with the untreated animals. The surfactant decreased neutrophil accumulation, increased fibroblast proliferation, and decreased changes in the volume of type I epithelial cells. Surfactant-treated animals also demonstrated better preservation of endothelial cell integrity. These responses indicate ameliorating effects of the surfactant on the pulmonary response to hyperoxia, including protection against epithelial and endothelial cell destruction. Significant interstitial inflammation and fibroblast proliferation remained, however, in surfactant-treated lungs exposed to continuous hyperoxia.

Salmonella is a foodborne zoonotic pathogen causing diarrhoeal disease to humans after consuming contaminated water, animal, and plant products. The bacterium is the third leading cause of human death among diarrhoeal diseases worldwide. Therefore, human salmonellosis is of public health concern demanding integrated interventions against the causative agent, Salmonella enterica. The prevention of salmonellosis in humans is intricate due to several factors, including an immune-stable individual infected with S. enterica continuing to shed live bacteria without showing any clinical signs. Similarly, the asymptomatic Salmonella animals are the source of salmonellosis in humans after consuming contaminated food products. Furthermore, the contaminated products of plant and animal origin are a menace in food industries due to Salmonella biofilms, which enhance colonization, persistence, and survival of bacteria on equipment. The contaminated food products resulting from bacteria on equipment offset the economic competition of food industries and partner institutions in international business. The most worldwide prevalent broad-range Salmonella serovars affecting humans are Salmonella Typhimurium and Salmonella Enteritidis, and poultry products, among others, are the primary source of infection. The broader range of Salmonella serovars creates concern over multiple strategies for preventing and controlling Salmonella contamination in foods to enhance food safety for humans. Among the strategies for preventing and controlling Salmonella spread in animal and plant products include biosecurity measures, isolation and quarantine, epidemiological surveillance, farming systems, herbs and spices, and vaccination. Other measures are the application of phages, probiotics, prebiotics, and nanoparticles reduced and capped with antimicrobial agents. Therefore, Salmonella-free products, such as beef, pork, poultry meat, eggs, milk, and plant foods, such as vegetables and fruits, will prevent humans from Salmonella infection. This review explains Salmonella infection in humans caused by consuming contaminated foods and the interventions against Salmonella contamination in foods to enhance food safety and quality for humans.

Symphysanodon disii was described in 2008 from a single specimen collected in October 1999 from the Gulf of Aqaba. More recently, March 2008, four additional specimens of this species were collected in the Gulf. The following characters in combination distinguish S. disii from the other species in the genus: parapophyses on the first caudal vertebra, tubed scales in the lateral line 48 to 50, total number of first-arch gillrakers 34 to 37, sum of total number of gillrakers plus lateral-line scales (in individual specimens) 82 to 87, pectoral-fin rays 16 or 17, fleshy orbit diameter 7.2 to 8.3 % SL, and second anal-spine length 8.3 to 9.7 % SL. We redescribe S.disii, comment on S. pitondelafournaisei from the southwestern Indian Ocean off Reunion Island, and discuss sexual dimorphism in the genus.

In the new issue, our scientific journal offers you thirteen scientific articles. As always, we try to offer a wide variety of topics and areas and follow current trends in the history of science and technology. In the article by Olha Chumachenko, оn the basis of a wide base of sources, the article highlights and analyzes the development of research work of aircraft engine companies in Zaporizhzhia during the 1970s. The existence of a single system of functioning of the Zaporizhzhia production association “Motorobudivnyk” (now the Public Joint Stock Company “Motor Sich”) and the Zaporizhzhia Machine-Building Design Bureau “Progress” (now the State Enterprise “Ivchenko – Progress”) has been taken into account. Leonid Griffen and Nadiia Ryzheva present their vision of the essence of technology as a socio-historical phenomenon. The article reveals the authors' vision of the essence of the technology as a sociohistorical phenomenon. It is based on the idea that technology is not only a set of technical devices but a segment of the general system – a society – located between a social medium and its natural surroundings in the form of a peculiar social technosphere, which simultaneously separates and connects them. Definitely the article by Denis Kislov, which examines the period from the end of the XVII century to the beginning of the XIX century, is also of interest, when on the basis of deep philosophical concepts, a new vision of the development of statehood and human values raised. At this time, a certain re-thinking of the management and communication ideas of Antiquity and the Renaissance took place, which outlined the main promising trends in the statehood evolution, which to one degree or another were embodied in practice in the 19th and 20th centuries. A systematic approach and a comparative analysis of the causes and consequences of those years’ achievements for the present and the immediate future of the 21st century served as the methodological basis for a comprehensive review of the studies of that period. The article by Serhii Paliienko is devoted to an exploration of archaeological theory issues at the Institute of archaeology AS UkrSSR in the 1960s. This period is one of the worst studied in the history of Soviet archaeology. But it was the time when in the USSR archaeological researches reached the summit, quantitative methods and methods of natural sciences were applied and interest in theoretical issues had grown in archaeology. Now there are a lot of publications dedicated to theoretical discussions between archaeologists from Leningrad but the same researches about Kyiv scholars are still unknown The legacy of St. Luke in medical science, authors from Greece - this study aims to highlight key elements of the life of Valentyn Feliksovych Voino-Yasenetskyi and his scientific contribution to medicine. Among the scientists of European greatness, who at the turn of the XIX and XX centuries showed interest to the folklore of Galicia (Halychyna) and Galician Ukrainians, contributed to their national and cultural revival, one of the leading places is occupied by the outstanding Ukrainian scientist Ivan Verkhratskyi. He was both naturalist and philologist, as well as folklorist and ethnographer, organizer of scientific work, publisher and popularizer of Ukrainian literature, translator, publicist and famous public figure. I. H. Verkhratskyi was also an outstanding researcher of plants and animals of Eastern Galicia, a connoisseur of insects, especially butterflies, the author of the first school textbooks on natural science written in Ukrainian. A new emerging field that has seen the application of the drone technology is the healthcare sector. Over the years, the health sector has increasingly relied on the device for timely transportation of essential articles across the globe. Since its introduction in health, scholars have attempted to address the impact of drones on healthcare across Africa and the world at large. Among other things, it has been reported by scholars that the device has the ability to overcome the menace of weather constraints, inadequate personnel and inaccessible roads within the healthcare sector. This notwithstanding, data on drones and drone application in Ghana and her healthcare sector in particular appears to be little within the drone literature. Also, little attempt has been made by scholars to highlight the use of drones in African countries. By using a narrative review approach, the current study attempts to address the gap above. By this approach, a thorough literature search was performed to locate and assess scientific materials involving the application of drones in the military field and in the medical systems of Africans and Ghanaians in particular. The paper by Artemii Bernatskyi and Vladyslav Khaskin is devoted to the analysis of the history of the laser creation as one of the greatest technical inventions of the 20th century. This paper focuses on establishing a relation between the periodization of the stages of creation and implementation of certain types of lasers, with their influence on the invention of certain types of equipment and industrial technologies for processing the materials, the development of certain branches of the economy, and scientific-technological progress as a whole. The paper discusses the stages of: invention of the first laser; creation of the first commercial lasers; development of the first applications of lasers in industrial technologies for processing the materials. Special attention is paid to the “patent wars” that accompanied different stages of the creation of lasers. A comparative analysis of the market development for laser technology from the stage of creation to the present has been carried out. Nineteenth-century world exhibitions were platforms to demonstrate technical and technological changes that witnessed the modernization and industrialization of the world. World exhibitions have contributed to the promotion of new inventions and the popularization of already known, as well as the emergence of art objects of world importance. One of the most important world events at the turn of the century was the 1900 World's Fair in Paris. Thus, the author has tried to analyze the participation of representatives of the sugar industry in the World's Fair in 1900 and to define the role of exhibitions as indicators of economic development, to show the importance and influence of private entrepreneurs, especially from Ukraine, on the sugar industry and international contacts. The article by Viktor Verhunov highlights the life and creative path of the outstanding domestic scientist, theorist, methodologist and practitioner of agricultural engineering K. G. Schindler, associated with the formation of agricultural mechanics in Ukraine. The methodological foundation of the research is the principles of historicism, scientific nature and objectivity in reproducing the phenomena of the past based on the complex use of general scientific, special, interdisciplinary methods. For the first time a number of documents from Russian and Ukrainian archives, which reflect some facts of the professional biography of the scientist, were introduced into scientific circulation. The authors from Kremenchuk National University named after Mykhailo Ostrohradskyi presented a fascinating study of a bayonet fragment with severe damages of metal found in the city Kremenchuk (Ukraine) in one of the canals on the outskirts of the city, near the Dnipro River. Theoretical research to study blade weapons of the World War I period and the typology of the bayonets of that period, which made it possible to put forward an assumption about the possible identification of the object as a modified bayonet to the Mauser rifle has been carried out. Metal science expert examination was based on X-ray fluorescence spectrometry to determine the concentration of elements in the sample from the cleaned part of the blade. In the article by Mykola Ruban and Vadym Ponomarenko on the basis of the complex analysis of sources and scientific literature the attempt to investigate historical circumstances of development and construction of shunting electric locomotives at the Dnipropetrovsk electric locomotive plant has been made. The next scientific article continues the series of publications devoted to the assessment of activities of the heads of the Ministry of Railways of the Russian Empire. In this article, the authors have attempted to systematize and analyze historical data on the activities of Klavdii Semyonovych Nemeshaev as the Minister of Railways of the Russian Empire. The article also assesses the development and construction of railway network in the Russian Empire during Nemeshaev's office, in particular, of the Amur Line and Moscow Encircle Railway, as well as the increase in the capacity of the Trans-Siberian Railway. The article discusses K. S. Nemeshaev's contribution to the development of technology and the introduction of a new type of freight steam locomotive for state-owned railways. We hope that everyone will find interesting useful information in the new issue. And, of course, we welcome your new submissions.

The quantity of municipal solid waste (MSW) generation is escalating at an alarming rate with every passing year alongside the modernization of our economy. Unfortunately, the majority of this waste remains uncollected or ends up in open dumping and followed by uncontrolled burning. Citing the deep-rooted consequences, open dumping should be absolutely abandoned and scientific interventions should be aggressively exercised to reclaim the municipal brownfields. The present research work undertook the judicial task of assessing the comparative feasibility of biomining and scientific capping as a technology selection for reclamation of about a decade old 120 million tons of waste chunk laying at Jawahar Nagar dump yard. Primary dump samples were collected from various locations, considering depth as a variable. While leachate and groundwater samples were collected from Malkaram lake and preinstalled borewells receptively. Additionally, the ambient air quality and noise level also been ascertained within the buffer zone. The blended representative solid sample was segregated using a 70 mm mesh size trommel into organic and inorganic fractions. The organic fraction was composted using a lab-scale aerobic static pile composting (ASPC) while the trommel reject was processed as refuse derived fuel (RDF). Evidently, the compost lagged quality and depicted nutrient deficiency. While the burning of RDF produced siloxane gas, significantly due to elevated silicon level in the primary waste. Furthermore, due to the prolonged leaching tenure and seasonal dilution, the concentration of legacy leachate was relatively weaker. Borewell samples collected from a depth of 20 feet also portrayed minor contamination up to 500 meters horizontal radius. The issue of leachability can solely be resolved with the capping of the existing dump and the end product quality derived from the biomining process is highly questionable. Thus, handling such large quantity capping is a befitting option over biomining for Jawahar Nagar dumpsite. INTRODUCTION Presently, in India due to rapid urbanization and industrialization, the generation of MSW has been increasing tremendously and also expected to continue a similar trend in the future (Scott, 1995; Bhat et al., 2017; Sethurajan et al., 2018; Sharma et al., 2018). Annually, the comprehensive urban MSW generation in India is more than 62 million tons. Metro cities are the mammoth contributor of the entire chunk and waste production had already reached an alarming figure of 50,000 tonnes/day. While the waste generation from the tier 2 cities is also rigorously escalating and presently contribute up to 20,000 tones/day (Sharma et al., 2018). A study conducted by the central pollution control board (CPCB) revealed MSW generation in India is increasing at a distressing rate of 5 % per annum with a sharp escalation in the quantities of domestic hazardous waste (Sharma et al., 2018). With major financial constraints, inefficacy of collection, treatment, and disposal incurs further reasons to worry. So far India has miserably failed to set up wholesome source segregation and collection method. Presently, the country spends more than 60% of its annual waste management budget only in collection. Besides, only 20% or less of the collected materials are scientifically handled and treated. Citing the statistics, it is evident that the majority of the MSW is simply gets dumped on the low laying grounds located somewhere on the outskirts of the cities. The precipitation, infiltration, surface water runoff, bird menace, rodent interference etc. triggers the vulnerability of waste and leads to mal odor, ground and surface water contamination, human and environmental health deterioration (Jayawardhana et al., 2016). Further, the perseverance of the inorganic and inert fractions leads to soil contamination, poses a fire threat, and also may incur carcinogenicity and acute toxicity among the animals (Mir et al., 2021). There are numerous techniques for the reclamation and remediation of the dumpsites, includes processes such as capping and closure, in-situ vitrification, sub-surface cut-off walls, and waste biomining (Chakrabarti and Dubey, 2015; Thakare and Nandi, 2016). Waste biomining is a stable way to get rid of the entire range of problems associated with open dumping and reclaim valuable land (Kaksonen et al., 2017). There are several instances including reclamation of Mumbai Gorai dump yard by IL & FS Environment, 70 – 80 years old 12,00,000 tons of dump clearance by Nagar Nigam Indore within a minute span of 3 years and many more. But the process of biomining is highly sensitive and case-specific. The success of the process solely depends on factors such as characteristics of the waste, efficacy of the effective microorganism culture, acceptability of the processed end product at the local market etc. (Jerez, 2017; Banerjee et al., 2017; Venkiteela, 2020). Contrarily, though the scientific capping is not an end-to-end solution but still advisable in the cases where the quantity of waste is gigantic, land scarcity is prevalent, no nearby industries to consume the end products etc. Mehta et al. (2018) have also supported the above claim based on the assessment of locations specific MSW dump reclamation case studies. While in another Nagpur-based case study conducted by Ashootosh et al. (2020) reported the superiority of the biominingprocess over simple land capping due to the favorability of the local conditions. Capping eliminates the environmental interference and thereby reduces biosphere contamination and leachate generation. Further, it captivates rodent and vector breeding and thereby curtails the spreading of communicable diseases and improves aesthetics. But right consolidation through compaction and execution is utmost necessary in the above case. As non-compaction and faulty sloping will easily lead to heavy settlement and slope failure (Berkun et al., 2005; Al-Ghouti et al., 2021). The present study has been pursued with the primary objective to run a techno-commercial assessment between scientific capping and biomining. While the secondary objective was to ascertain the level of contamination and propose mitigative measures. MATERIALS AND METHODStudy Area Spanning over 350 acres of a precious piece of land at the outskirts of Hyderabad city, Jawahar Nagar dumping yard was brutally utilized by the Greater Hyderabad Municipal Corporation (GHMC) for open dumping for a prolonged tenure of 10 years. It housed nearly 12 lakh metric tons of heterogeneous solid and domestic hazardous waste and continues polluting until 2015, until the Ramky group was offered to cap the legacy dumping and scientifically handle the site. The present study has been facilitated at Hyderabad Municipal Solid Waste Limited, formerly known as Jawahar Nagar dump yard to analyze and assess the feasibility of bio-mining as handling and management alternate to the existing practice of scientific capping. The epicenter of processing and disposal facility is lying approximately on the cross-section of 17°31'24.45"N and 78°35'23.37"E. As per the contract, the comprehensive legacy dumping to be capped in three phases over about 150 acres of area and Ramky has significantly entered the phase two of the operation only within a span of five years by successfully capping more than half of the legacy footprint. Sampling Methodology The waste pile was divided into three layers namely, base, middle, and top. A uniform amount of sample was collected from the successive layers of all five different corners which cover north, south, east, west, and central of the garbage pile. Sampling inspections were performed using a manual auger besides large samples were collected using a JCB excavator. The top six-inch layer of the pile was removed to avoid any contamination while collecting the samples and 5-10 kg of sample was collected from each of the locations. Further, intermediate and bottom layer samples were collected by digging a 500 mm diameter hole through the heap. A composite was prepared by a homogenized blending of all the fifteen grub samples. The blend was distributed into four equal quadrants and the top and bottom quadrants were eliminated diagonally while the left-over quadrants were mixed thoroughly. This process was repeated until a sample of the required bulk of 20 kg is obtained. Surface and subsurface water samples from borewell were collected in and around the facility. Piezometric monitoring borewells located near the landfills were utilized for the subsurface sample collection. While a rainwater pond turned leachate lake named Malkaram was determined as the primary source for leachate collection. Buffer samples were collected from Ambedkar Nagar, the nearby colony exiting at a distance of only 300 meters. Lab-scale Experimentation The representative sample was characterized for composition and further screened through a 70 mm mesh size trommel. The trommel permeate was considered as the organic fraction while the reject was mostly inorganics and inert. The organics were subjected to ASPC. The quantity of the air required is arrived using the method delineated below (Figure 1). MSW Pile size: 2m x 0.5m x 0.5m Volume of pile: 0.5 m3 Average Density of MSW: 620 Kg/m3 Weight of pile: 310 Kg Nitrogen required for matured compost: 9300 mg/kg dry : 9300 X 310 mg : 2.88 x 106 mg : 2.88 Kg Total air required: 2.88 x 100/76 [as Nitrogen in air is 76% by weight] : 3.79 Kg of dry air : 3.79/1.225 m3 [@ 15 deg C density of air 1.225 kg/m3] : 3.1 m3 This air is to be supplied for 100 min / day for 0.5 m pile Air flow rate required: 3.1 x 60/100 = 1.86 m3/h (for practical purpose a flowrate of 2 m3/h was maintained). The maturation period was considered as 28 days and post-maturation, the stabilized material was further cured for 24 hours and screened using 12 mm and 4 mm trommel respectively to obtain the desired product quality and particle size. Whereas, the trommel reject was evenly spreader on the copper trays and dried in an oven at 1050C for 2 hours. The dried material was micronized to the size of 50 mm or below using a scissor and inert such as glass, sand, stone etc. were segregated manually (Mohan and Joseph, 2020). Concurrently, a bench-scale capped landfill prototype was built using the below-mentioned procedure to evaluate the factors such as settlement and slope stability. A 30 mm thick low permeable soil was laid on the top of the waste, followed by a 60 mm layer of compacted clay liner (CCL). Each join between successive liner material was closely monitored. A 1.5 mm thick HDPE liner was placed on the top of the CCL. A 285 GSM geotextile membrane was placed as the successive above layer followed by a 15 mm thick drainage media layer. A further layer of geotextile membrane was placed on top of the drainage media for better stabilization, grip, and strength. The top vegetative soil layer of 45 mm thickness was laid off on top of the geotextile media and St. Augustine grass was rooted (Cortellazzo et al., 2020; Ashford et al., 2000). 2.4 Sample Analysis pH, Electrical Conductivity (EC) and Turbidity of the samples were analyzed using pH, EC-TDS, and Nephelometer of Mettler Toledo. The pH meter was calibrated with the buffer solution of 4.0, 7.0 & 9.12 at a controlled temperature. EC-TDS meter was calibrated with 0.1 M KCL having 12.8 mS/cm of conductivity. Nephelometer was calibrated with Formazine solution of 10 & 100 NTU. Total Dissolved Solids (TDS), (mg/L) was performed using the gravimetric method at 1800C in the oven. Titrimetric parameters such as Total Alkalinity as CaCO3 (mg/L), Total Hardness as CaCO3 (mg/L), Chloride as Cl- (mg/L), Calcium as Ca2+ (mg/L), Residual Free Chlorine (RFC), (mg/L) were analyzed using APHA (American Public Health Associations) method, 23rd Edition, 2017. Total Kjeldahl Nitrogen (mg/L) and Ammonical Nitrogen (mg/L) were performed through distillation followed by titration with H2SO4 as a titrant. Sulphide as S2- was done with the Iodometric method after distillation. Each titrimetric parameter was analyzed in triplicate after standardizing the titrant with required reagents and crossed checked by keeping a check standard. Sodium as Na (mg/L) and Potassium as K (mg/L) were performed using Flame Photometer. The photometer was calibrated with different standards from 10 to 100 (mg/L) standard solutions. The leachate sample was diluted enough to get the value within the standard range and cross-checked with check standards at the same time. Chemical Oxygen Demand (COD), (mg/L) was performed using the open reflux method for 2 hours at 1500C in COD Digestor. Biochemical Oxygen Demand (BOD), (mg/L) was performed using the alkali iodide azide method for 3 days. The samples were kept in a BOD incubator at 270C for 3 days. It was kept in duplicate to have a check on quality control. Sulphate was analyzed by the gravimetric method instead of turbidimetric or through UV-Visible spectrophotometer as its concentration was found more than 40 mg/L. Nitrate as NO3- was analyzed after filtration at 220-275 nm, while Hexavalent Chromium as Cr6+ was analyzed at 540 nm in the UV-Vis. Parameters like Cyanide as CN-, Fluoride as F-, and Phenolic Compounds were gone through a distillation process followed by UV-Vis. The distillation process ensures the removal of interferences presents either positive or negative. For the parameters like Total Iron or Ferric Iron, the samples were digested properly with the required reagents on the hot plate before analyzing in UV-Vis. For the metal analysis the water samples were digested at a temperature of 1000C using aqua regia as a media. The samples were digested to one-fourth of the volume on a hot plate. The recommended wavelengths as per APHA 3120 B were selected for each of the metals. The standard graph was plotted for each of the metals before analysis and crossed checked with the check standard at the same time. Parameters such as bulk density and particle size were performed through the certified beaker and sieve. The percentage of moisture content was estimated using the oven by keeping the compost sample for 2 hours at 1050C. C/N ratio was estimated through CHNS analyzer keeping sulfanilamide as a check standard. The analysis was performed by extracting the desired component in the desired solution prescribed in the method followed by converting the same from mg/L to mg/Kg. RESULTS AND DISCUSSION An exhaustive bench-study has been pursued and real-time samples were collected and analyzed for all possible parameters to determine the pros and cons attributed to both processes. The investigation begins by collecting the samples and concluded by impact assessment studies inclusive of the buffer zone. Both solid, liquid, and gaseous samples were precisely investigated to opt for the best solution. A detailed finding of the investigation is summarized below. Primarily, the representative solid sample was characterized through a manual separation process and the results are portrayed in Figure 1. Compost Characterization ASPC of the organic fraction has resulted in a recovery of 46.7% of the initial load. While 53.3% of the influent mass were inert and barely degradable fraction contributes to reject, the rest 4.1% is miscellaneous process loss. The processed compost was extensively analyzed including for metal contamination and the same is tabulated in Table 1. The value of C/N ratio, OC, TN, K2O, P2O5, and NPK evidently portrays the shortcoming in terms of nutrient availability. Though it is highly enriched in organic carbon and thus the same can be effectively utilized as a soil preconditioner. Ayilara et al. (2020) also reported a similar finding, where the city compost sourced from MSW lagged major plant nutrients. RDF Characterization Processed trommel rejects constitute cloth, rexine, leather, jute, paper, plastics, coir and other inert contributed to RDF. The fraction of inert was as high as 37.2% of the overall RDF mass and it mostly constituted glass and sand. The combined weight of sand and glass fragments contributed 73.5% of the total inert, while the rest was stone and small brickbats. The higher level of silicon associated with the presence of glass and sand yielded siloxane and triggered the possibility of kiln corrosion. A detailed RDF analysis report is enclosed in Table 2. The values explicitly portray the quality of RDF is moderately lower and higher salts concentration is extremely prevalent. With relatively lower NCV and such high salt concentration, the above specimen will certainly pose a corrosion threat to the kiln and shall be either neglected as kiln feed or can be utilized after dilution with Grade III RDF quality. Further, such high ash generation will also induct high transportation and landfill charges. Leachate Characterization The Malkaram leachate lake is the end result of prolonged, slow, and steady mixing of the legacy leachate through the existing fissure cracks in the sheath rock bottom profile. Apparently, the concentration of leachate is significantly lower due to the dilution. Samples were analyzed in triplicates and the mean value is tabulated here in Table 3. The metal concertation and rest of the parameter values are well within the secondary treatment influent range, except for TDS. Thus, a modular aerobic biological treatment unit such as moving bed biofilm bioreactor (MBBR) or membrane bioreactor (MBR) would be a well-suited pick. However, a reverse osmosis (RO) system needs to be installed to get rid of the high TDS content. The permeate of RO can be reused back into the system. Whereas, the reject can be converted into dried powder through forced evaporation mechanisms. The higher concentration of salts in RDF collaterally justifies the elevated TDS level in leachate. In a leachate impact assessment study performed by El-Salam and Abu-Zuid (2015) the reported BOD/COD ratio of 0.69 is greater than double the value of 0.301 reported in Table 3. Though the difference in both the values are quite high, it is relatable and justifiable by the huge age difference of the source waste. The primarily characterized data is of a fresh leachate generated from regular MSW, while the later one is from a decade old waste that barely has any unstabilized organic content. Groundwater Contamination The obvious reason for downward leachate infiltration and osmotic movement facilitates groundwater contamination. Both surface and subsurface water samples were collected within the dump yard and the buffer zone and analyzed using the standard methods. The results are portrayed in Table 4. The slightly alkaline pH of the borewell sample is an indication of the ongoing anaerobic process. The dissolved oxygen value of 3.5 mg/L further validates the correlation. Higher TDS and hardness values are self-indicative of elevated salt concentration in source waste. Eventually, the same interfered with the RDF quality. Positively in the case of all the parameters, a successive decrement in pollution concentration has been spotted from dump ground towards the buffer zone. In a similar study conducted by Singh et al. (2016) at Varanasi, Uttar Pradesh the reported concentration of the parameters is significantly higher than reported in Table 4. The basic reason behind variation is the dissimilarities of the local soil profile. The sandy and clay loam soil profile of Varanasi allows a greater rate of percolation and infiltration. While the bottom sheath rock profile at Jawahar Nagar permits the only a minute to little percolation rate. The difference in percolation rate is directly correlated to the concentration levels in this case. Contrarily, Kurakalva et al. (2016) have reported much-elevated pollutant concertation both in ground and surface water for a study conducted at the same site in 2016. The higher concentration is relatable to the fact of the non-closure of the open dump back then. Capping activity had at Jawahar Nagar gained its pace 2018 onwards and capping for the primary section of 70 acres got concluded only during mid of 2019. Due to the decrement in runoff and percolation, the quality of both surface and subsurface water has improved drastically. Impact Assessment The odor and groundwater contamination are two of the primary issues that triggered a massive public agitation initially. The root causes of both the issues are identified as rainwater percolation and anaerobic digestion respectively. Eventually, the completion of the capping process would resolve both the problems effectively. Other non-tangential impacts include nausea; headache; irritation of the eye, nasal cavity, and throat; diarrhoeal diseases; vector-borne disease, cattle toxicity etc. Scientific capping can easily cater as the wholesome solution for all (Cortellazzo et al., 2020). Yu et al. (2018) had performed an extensive study to comprehend the relativity of respiratory sickness and MSW borne air pollution. The study made a couple of dreadful revelations such as gases released due to the anaerobic digestion of MSW such as methane, hydrogen sulphide, and ammonia incur detrimental impact on Lysozyme and secretory immunoglobulin A (SIgA). While SO2 was reported as the lung capacity and functionality reducer. Further, a gender-specific study executed by the same research group revealed, air pollution impacts more severely on male children than the female and retards immune functions. Presently, the area of 351 acres has been developed as Asia’s one of the largest state of the art municipal solid waste processing and disposal facility by Ramky Enviro Engineers Limited. This ensured zero dumping and no further environmental interventions. As legal compliance, the facility monitors the quality of groundwater and ambient air quality in and around the facility on monthly basis to assure the biosafety. The variation in concentration of various monitoring parameters between 2012 to 2020 is summarized in Figure 2. The concentration of each of the parameters are showcased in ppm and a standard equipment error was settled at 3% for respirable dust sampler and multi-gas analyzer (Taheri et al., 2014). Despite all parameter values have gradually increased except for methane, the facility still managed to maintain them well under the regulatory limits. The decrement in methane concentration is directly correlated to the practice of aerobic composting and aeration-based secondary treatment that prevented the formation of the anaerobic atmosphere and henceforth methane generation. While for the rest of the parameters the increment in values is quite substantial and predictable due to the sudden escalation in MSW generation in the past decade in correlation with Gross domestic product (GDP) enhancement. The observed and interpreted impacts due to the elevated pollutant level are in-line with the georeferenced findings reported by Deshmukh and Aher (2016) based on a study conducted at Sangamner, Maharashtra. CONCLUSION The study critically analyzed and investigated every techno-environmental and socio-economic aspect correlated to open dumping. The bench-scale experimentation revealed the efficiency of the single liner scientific capping is fair enough to eliminate any further rainwater infiltration, however, it has no control over the generation of leachate due to the inherent moisture. Internal moisture related issue was anyhow compensated with pertinent compaction prior to dispose of the waste. Contrarily, both the products derived through the biomining process namely, compost and RDF lagged quality due to scantier nutrient content and higher salt and silicon content respectively. Besides, impact assessment studies concede the pollutant concentration in groundwater in and around the plant has drastically diminished post-July 2019 due to the partial completion of waste capping. It also abetted lowering the dust and odor issues relatively in the surrounding. ACKNOWLEDGMENT The authors would like to sincerely acknowledge GHMC, Hyderabad Integrated Municipal Solid Waste Limited, and Ramky Enviro Engineers Limited for enabling us to pursue the sample collection and other necessary onsite activities. Further, the authors would like to register profound acknowledgment to EPTRI for supporting us with the essential experimental facilities. REFERENCES Sharma, A., Gupta, A.K., Ganguly, R. (2018), Impact of open dumping of municipal solid waste on soil properties in mountainous region. 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The mounting human population and the massive amount of waste generated from the same is receiving particular attention towards valorisation of waste. According to the annual report of FAO, the human consumption of fish protein has reached 87% in 2016 from 67% during 1960s [1]. Aquaculture only has contributed to 5.8% annual growth rate among food sectors in the past decade. In this milieu, disposal of fish visceral waste is becoming a major menace to fishery industries exerting a great economic and environmental impact. Being perishable in nature, the organic portion of the waste decomposes rapidly and acts as a breeding ground for microbes. Moreover, the hefty and indiscriminate use of antibiotics and disinfectants in farmed animals is developing resistant strains, thus raising environment and ecological concerns. In order to solve such problem, the present investigation focussed upon employing the visceral trypsin as a cell dissociating agent. The efficacy of trypsin obtained from viscera of Labeo rohita upon KB cell line (Doubling time 50 hrs.) was assessed in terms of cell viability. The cytotoxic effect of the visceral trypsin at 0.01%, 0.1% and 1% concentration were investigated at three time points (10 sec, 15 sec and 20 sec). Commercial (bovine) trypsin was considered as control. A time dependent decrease in cell viability upon gradually increasing the concentration was observed in all groups of treatment. The lowest reduction in cell viability (2%) was observed with 1% concentration at 15 sec and 20sec. Although, commercial trypsin was found more efficient than trypsin isolated from waste during this study but the potency of visceral trypsin observed cannot be ruled out. Thus, the application of this enzyme as a cell-dissociating agent suggested it as a comparable candidate with commercial trypsin.

Small ruminants (sheep and goat) immensely contribute to the Indian meat food basket; yet regional preference for either sheep or goat meat varies. Owing to the differences in the market prices, these two closely resembling species meats are often misrepresented interchangeably. Prevention of such fraudulent practices requires authentic and sensitive speciation tools. A novel mitochondrial D-loop based species-specific polymerase chain reaction (PCR) technique was developed for the precise detection of origin of sheep and goat meats. Species-specific primers (SSP-G and SSP-S) developed for PCR in this study amplify 229 and 425 base pairs products specific to goat and sheep species. An additional herteroplasmic PCR product of size 350 bp was evident only in sheep. Primers designed in this study were checked for the absence of cross-species amplification in closely related animal species and was successfully applied for the authentication of a forensic case. Our results indicated prerogative applications of the novel species-specific PCR for the authentic identification of origin of sheep and goat species thereby aiding in the prevention of the menace of misrepresentation of meats.