Literatura científica selecionada sobre o tema "Agroinfiltratio"
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Artigos de revistas sobre o assunto "Agroinfiltratio"
Liu, Pei-Feng, Yanhan Wang, Robert G. Ulrich, Christopher W. Simmons, Jean S. VanderGheynst, Richard L. Gallo e Chun-Ming Huang. "Leaf-Encapsulated Vaccines: Agroinfiltration and Transient Expression of the AntigenStaphylococcal EndotoxinB in Radish Leaves". Journal of Immunology Research 2018 (2018): 1–9. http://dx.doi.org/10.1155/2018/3710961.
Texto completo da fonteAmbrós, Silvia, Choaa El-Mohtar, Susana Ruiz-Ruiz, Leandro Peña, José Guerri, William O. Dawson e Pedro Moreno. "Agroinoculation of Citrus tristeza virus Causes Systemic Infection and Symptoms in the Presumed Nonhost Nicotiana benthamiana". Molecular Plant-Microbe Interactions® 24, n.º 10 (outubro de 2011): 1119–31. http://dx.doi.org/10.1094/mpmi-05-11-0110.
Texto completo da fonteWang, Zhiquan, Xiaoyang Xu, Longjie Ni, Jinbo Guo e Chunsun Gu. "Efficient virus-induced gene silencing in Hibiscus hamabo Sieb. et Zucc. using tobacco rattle virus". PeerJ 7 (12 de agosto de 2019): e7505. http://dx.doi.org/10.7717/peerj.7505.
Texto completo da fonteBridgeland, Aya, Sudip Biswas, Nikolaos Tsakirpaloglou, Michael J. Thomson e Endang M. Septiningsih. "Optimization of gene editing in cowpea through protoplast transformation and agroinfiltration by targeting the phytoene desaturase gene". PLOS ONE 18, n.º 4 (5 de abril de 2023): e0283837. http://dx.doi.org/10.1371/journal.pone.0283837.
Texto completo da fonteDebler, Johannes W., Bernadette M. Henares e Robert C. Lee. "Agroinfiltration for transient gene expression and characterisation of fungal pathogen effectors in cool-season grain legume hosts". Plant Cell Reports 40, n.º 5 (3 de abril de 2021): 805–18. http://dx.doi.org/10.1007/s00299-021-02671-y.
Texto completo da fonteDickinson, Christopher C., Alexandra J. Weisberg e John G. Jelesko. "Transient Heterologous Gene Expression Methods for Poison Ivy Leaf and Cotyledon Tissues". HortScience 53, n.º 2 (fevereiro de 2018): 242–46. http://dx.doi.org/10.21273/hortsci12421-17.
Texto completo da fonteChong, Xinran, Yue Wang, Xiaoyang Xu, Fan Zhang, Chuanyong Wang, Yanwei Zhou, Ting Zhou, Yunlong Li, Xiaoqing Lu e Hong Chen. "Efficient Virus-Induced Gene Silencing in Ilex dabieshanensis Using Tobacco Rattle Virus". Forests 14, n.º 3 (28 de fevereiro de 2023): 488. http://dx.doi.org/10.3390/f14030488.
Texto completo da fonteChiba, Sotaro, Kamal Hleibieh, Alice Delbianco, Elodie Klein, Claudio Ratti, Véronique Ziegler-Graff, Salah Bouzoubaa e David Gilmer. "The Benyvirus RNA Silencing Suppressor Is Essential for Long-Distance Movement, Requires Both Zinc-Finger and NoLS Basic Residues but Not a Nucleolar Localization for Its Silencing-Suppression Activity". Molecular Plant-Microbe Interactions® 26, n.º 2 (fevereiro de 2013): 168–81. http://dx.doi.org/10.1094/mpmi-06-12-0142-r.
Texto completo da fonteTu, Liqin, Shuhua Wu, Danna Gao, Yong Liu, Yuelin Zhu e Yinghua Ji. "Synthesis and Characterization of a Full-Length Infectious cDNA Clone of Tomato Mottle Mosaic Virus". Viruses 13, n.º 6 (1 de junho de 2021): 1050. http://dx.doi.org/10.3390/v13061050.
Texto completo da fonteSindarovska, Yana, e Mykola Kuchuk. "Long-Term Potato Virus X (PVX)-Based Transient Expression of Recombinant GFP Protein in Nicotiana benthamiana Culture In Vitro". Plants 10, n.º 10 (15 de outubro de 2021): 2187. http://dx.doi.org/10.3390/plants10102187.
Texto completo da fonteTeses / dissertações sobre o assunto "Agroinfiltratio"
Abe, Valeria Yukari. "Expressão transiente de genes de Phakopsora pachyrhizi em genótipos resistentes de soja visando a identificação de genes de avirulência". Universidade Federal de Viçosa, 2012. http://locus.ufv.br/handle/123456789/4404.
Texto completo da fonteCoordenação de Aperfeiçoamento de Pessoal de Nível Superior
Brazil is the second world soybean producer. Currently, the main limiting factor in this crop production is the Asian soybean rust (ASR) whose etiologic agent is the fungus Phakopsora pachyrhizi. The rust fungi are obligate parasites that during their interaction with the plant, they secrete effector proteins that manipulate host metabolism and interfere with their defense responses. Some of these effector proteins, called Avr proteins, are recognized by encoded proteins by resistant R genes, which usually trigger a hypersensitivity response (HR) and resistance phenotype. At least, there are five described R genes (Rpp1 to Rpp5) that confer resistance to ASR and several genes that encode secreted proteins by this fungus were recently identified. However, the effector proteins (Avr) recognized by encoded proteins by Rpp genes were not identified yet. Since there is not a transformation assay protocol for P. pachyrhizi, a strategy to identify this fungus Avr proteins is the transient expression of R proteins in resistant varieties cytoplasm and the observation of a possible HR response. Thus, the specific objectives of this work were: to try to establish a methodology for transient expression in soybean by agroinfiltration using the gene GUSPlus as reporter gene; to establish a protocol for translocation of effector proteins by the type III secretion system (SST3) of Pseudomonas syringae pv. glycinea race 4 (Psg4), and also to evaluate the effector activity of candidate genes in soybean resistant genotypes to the isolate PPUFV02 of P. pachyrhizi. There was no expression of the gene GUSPlus in Agrobacterium tumefaciens strain EHA105 infiltrated soybean leaves, while using the same inoculum preparation and concentration of bacterial cells, there was a consistent expression of the gene GUSPlus in tobacco. This result derailed the use of agroinfiltration in the functional study of candidate genes in soybean effectors. All soybean genotypes evaluated were susceptible to Psg4, demonstrating that the viability to use this bacterial on functional analysis of candidate effector proteins mediated by SST3. Better symptoms reproducibility was observed with inoculation by vacuum infiltration of Psg4 in a bacterial concentration of OD600 = 0,01, for allowing a gradually symptoms analysis. The encoded protein by avrB gene is recognized by the RPG1 gene product, which is present in some genotypes of soybean. The construction pVSP61-avrB was able to induce HR in the genotype Williams 82, that contains the corresponding gene RPG1 and to induce it in the genotypes Conquista and PI 459025. This result allowed the use of this construction as a positive control for functional analysis of P. pachyrhizi putative effector proteins. Because of this, 12 sequences were cloned into vector pEDV6. This vector allows the expression of proteins of interest fused to secretion signal sequences by SST3, aiming its subsequent translocation into the cytosol. Nine from the constructions with pEDV6-PHPA_RSP transformed into Psg4 were submitted to functional analysis. The inoculated plants varied in severity of observed symptoms and no HR phenotype was observed. Instead, it was observed reduction, increase or absence of a significant change in the symptoms evolution of genotype-dependent manner in treated plants. These studies allowed a first screening of P. pachyrhizi effector candidates, selecting the candidates PHPA_RSP_71 and PHPA_RSP_78 as the most promising candidates for further detailed analysis.
O Brasil é o segundo maior produtor mundial de soja. Atualmente, o principal fator limitante na produção desta cultura é a ferrugem asiática da soja (FAS), cujo agente etiológico é o fungo Phakopsora pachyrhizi. Os fungos causadores das ferrugens são parasitas obrigatórios que durante sua interação com a planta secretam proteínas efetoras que manipulam o metabolismo do hospedeiro e interferem com suas respostas de defesa. Algumas dessas proteínas efetoras, denominadas proteínas Avr, são reconhecidas pelas proteínas codificadas por genes de resistência R, o que desencadeia a resposta de hipersensibilidade (HR) e fenótipo de resistência. Já foram descritos pelo menos cinco genes R (Rpp1 a Rpp5) que conferem resistência a FAS e vários genes que codificam proteínas secretadas por esse fungo foram recentemente identificados. Todavia, ainda não foram identificadas as proteínas efetoras (Avr) reconhecidas pelas proteínas codificadas pelos genes Rpp. Como não existe ainda um sistema de transformação para P. pachyrhizi, uma estratégia para identificar as proteínas Avr desse fungo é a expressão transiente das proteínas efetoras candidatas no citoplasma de variedades resistentes e a observação do possível desencadeamento de resposta de HR. Desta forma, os objetivos específicos deste trabalho foram tentar estabelecer uma metodologia de expressão transiente em soja via agroinfiltração utilizando como gene repórter o gene GUSPlus; estabelecer um protocolo de translocação de proteínas efetoras via sistema de secreção tipo III (SST3) de Pseudomonas syringae pv. glycinea raça 4 (Psg4), e também avaliar a atividade efetora de genes candidatos em genótipos de soja resistentes ao isolado monopustular PPUFV02 de P. pachyrhizi. Não se observou a expressão do gene GUSPlus em folhas de soja agroinfiltradas com Agrobacterium tumefaciens estirpe EHA105, enquanto que utilizando do mesmo preparo de inóculo e concentração de células bacterianas, observou-se a expressão consistente do gene GUSPlus em tabaco. Este resultado invibializou o uso de agroinfiltração no estudo funcional de genes efetores candidatos na soja. Todos os genótipos de soja avaliados foram suscetíveis a Psg4, demonstrando a vialibilidade do uso desta bactéria na análise funcional de proteínas candidatas a efetores mediada por SST3. Melhor reprodutibilidade de sintomas foi observada com a inoculação por infiltração a vácuo de Psg4 numa concentração bacteriana de OD600=0,01, por permitir uma análise dos sintomas de forma gradual. O produto do gene avrB é reconhecido pela produto do gene RPG1, presente em alguns genótipos de soja. A construção pVSP61-avrB, foi capaz de induzir HR no genótipo Williams 82, que contêm o gene RPG1 correspondente, e nos genótipos Conquista e PI 459025. Este resultado permitiu o uso desta construção como controle positivo para a análise funcional de proteínas efetoras putativas de P. pachyrhizi. Assim, 12 sequências foram clonadas no vetor pEDV6. Este vetor permite a expressão de proteínas de interesse fusionadas a sequências-sinais de secreção via SST3, visando a sua posterior translocação para o citosol. Nove das construções com pEDV6-PHPA_RSP transformadas em Psg4 foram submetidas à análise funcional. As plantas inoculadas variaram quanto à severidade dos sintomas observados e não foi constatado fenótipo de HR. Ao invés disso, nos tratamentos foi observado redução, aumento ou ausência de alteração significativa na evolução dos sintomas, de maneira genótipo-dependente. Esses estudos permitiram uma primeira triagem de candidatos a efetores de P. pachyrhizi, selecionando os candidatos PHPA_RSP_71 e PHPA_RSP_78 como os mais promissores para estudos futuros mais detalhados.
Tah, Tapashree Schoelz James E. "Chloroplast GFP expression in tobacco plants agroinfiltrated with tobacco mosaic virus based vectors". Diss., Columbia, Mo. : University of Missouri--Columbia, 2009. http://hdl.handle.net/10355/6604.
Texto completo da fonteJoh, Lawrence Day. "High-level transient expression, extraction, and purification of recombinant [beta]-glucuronidase from agroinfiltrated lettuce /". For electronic version search Digital dissertations database. Restricted to UC campuses. Access is free to UC campus dissertations, 2005. http://uclibs.org/PID/11984.
Texto completo da fonteDegree granted in Biological Systems Engineering. On t.p. "[beta]" appears as Greek letter. Also available via the World Wide Web. (Restricted to UC campuses)
Bunwaree, Heemee Devi. "Implementation of a genetic screen for the identification of resistances to beet virus yellows". Electronic Thesis or Diss., Strasbourg, 2024. http://www.theses.fr/2024STRAJ042.
Texto completo da fonteSince the ban on neonicotinoids in the European Union, sugar beet production has been severely threatened by virus yellows (VY) epidemics. VY are caused by a complex of several aphid-transmitted viruses, among which the poleroviruses beet mild yellowing virus (BMYV) and beet chlorosis virus (BChV) are highly represented. In order to improve the screening of sugar beet varieties resistant or tolerant to viral yellows, we produced a recombinant virus, allowing easy and rapid visual discrimination between infected and healthy plants, without the need of additional equipment. It is a clone of BMYV capable of inducing the silencing of an endogenous gene via the phenomenon of virus induced gene silencing (VIGS), with infection manifesting as accelerated vein clearing of leaves, starting as early as ten days after agroinoculation. Molecular analyses revealed that the recombinant virus displays the same infectivity as the wild-type virus and that the insert is stable within the viral progeny, till at least five months post-infiltration. Our results also indicated that the percentage of VIGS-symptomatic plants is representative of the infection rate for each evaluated line. The use of this tool allowed us to visually identify one BMYV resistant and three partial resistant lines from forty-two sugar beet lines. Such lines represent interesting potential candidates for breeding programs. Thus, this work validates the use of a polerovirus as a VIGS vector, adapted to sugar beet, allowing large-scale, robust visual screenings for the identification of resistance genes or for functional studies
Bandyopadhyay, Amrita. "Analysis of the Arabidopsis Polyadenylation Factors PAP1, CstF64 and CstF77 and their characteristic inter-relationship". UKnowledge, 2009. http://uknowledge.uky.edu/gradschool_theses/601.
Texto completo da fonteLin, Yi-Jyun, e 林怡君. "Examination Effect of Accessory Protein in T-DNA transferring by Transient Agroinfiltration of Tobacco". Thesis, 2011. http://ndltd.ncl.edu.tw/handle/82128429955580164605.
Texto completo da fonte國立中興大學
生物科技學研究所
99
For the Agrobacterium-mediated plant transformation, the long journey for T-DNA transferring from bacteria till the final destination not only requires bacterial proteins, such as VirE2, VirE3, etc., but also is aided by various plant factors, including BTI1/BTI2/BTI3/Rab8, VIP1, VIP2, Ku70/Ku80, H2A, etc. Many reports had demonstrated that T1 progenies of transgenic Arabidopsis overproducing various accessory proteins exhibited higher transformation efficiency. Gene orthologs encoding the accessory proteins were isolated from rice and designated as “ET genes” for purpose of “enhance transformation”. Co-transformation strategy, infiltrate one Agrobacterium carries an ET gene and the other contains the mGFP5 reporter gene, was employed to evaluate transient transformation efficiency in tobacco. Several ET genes were already identified to be effective. In this study, ET gene expressed by 2X35S promoter mostly exerted better results than the previous constructs. Synergistic effects were observed when several ET genes were combined, with the best effect observed for mixture of VirE2, VIP1 and H2A genes. Moreover, to demonstrate that enhancement of transformation is caused by expression of ET proteins, truncated ET genes were generated. As a result, disrupt open reading frame of ET gene concomitantly diminish its ET effect. A preliminary test using floral dip of Arabidopsis revealed that BTI3 enhance permanent transformation frequency to be about ~2.2 fold. Besides, Agrobacterium EHA105 was transformed with plasmids to overproduce VirG proteins in its wildtype or constitutively active (N54D) version. VirG gene from C58, but not from LBA4404, was found to increase the “virulence” of EHA105.
Lin, Jia-Ying, e 林佳螢. "Development of an in Planta System to Monitor Phosphorus Status by Agroinfiltration and Agroinjection". Thesis, 2014. http://ndltd.ncl.edu.tw/handle/21081124561212623300.
Texto completo da fonte國立臺灣大學
園藝暨景觀學系
102
Phosphorous (P), one of the essential mineral nutrients for plant growth and development, is involved in the regulation of several physiological and biochemical processes in plants. In our study, it was found that, in the tobacco and tomato plants at vegetative growth stage under phosphorus deficiency treatment, the phosphate (Pi) concentration in shoot and root decreased rapidly, the chlorophyll fluorescence (Fv/Fm), chlorophyll content and shoot fresh weight decreased relatively slowly, while the root/shoot fresh weight ratio increased gradually. Furthermore, in tomato plants at reproductive growth stage under Pi starvation, the Pi concentration in fruit decreased rapidly, and then the fruit yield and total soluble solids decreased. Accordingly, it is shown that the vegetative growth of tobacco and tomato plants as well as the final yield and quality of tomato depend on whether phosphorus fertilizer is supplied sufficiently. It was further found that, while temporary phosphorus deficiency may immediately lead to the decrease of the Pi concentration in shoot or fruit, the shoot fresh weight or the fruit yield and quality may not be adversely impacted thereby so long as phosphorus fertilizer is timely supplemented to rapidly increase the Pi concentration in shoot or fruit. This indicates that the yield and quality of crops can be ensured by monitoring the nutritional status of the crops and supplying fertilizer as appropriate. By Agroinfiltration and Agroinjection, this study demonstrated that the expression of GUS reporter gene driven by tomato TPSI1 promoter can rapidly and truly reflect the phosphorus deficiency stress in tobacco leaf and tomato fruit. This indicates that the employed Agroinfiltration/Agroinjection transient expression system is useful in monitoring the Pi status in plants. Further, the aforesaid expression of GUS reporter gene is independent of the deficiency of the mineral nutrients other than phosphorus, indicating the specificity of the employed Agroinfiltration transient expression system. Because the application of the aforesaid system is convenient and leads to rapid and accurate reflection of phosphorus status in plants, the aforesaid system should be applicable to other crops or to monitor other mineral nutrient status if the tomato TPSI1 promoter is replaced by other appropriate promoters.
Ribeiro, Diana Margarida da Costa. "Optimization of transient expression procedures in Catharanthus roseus and Arabidopsis thaliana for subcellular localization studies". Master's thesis, 2010. http://hdl.handle.net/1822/15963.
Texto completo da fonteNowadays, the availability of much gene sequence information demands the development of tools for their fast characterization at the protein level, where function actually resides. Here, the interest in the characterization of certain of the known Arabidopsis class III peroxidase (Prx) genes, as well as the interest in the characterization of candidate genes implicated in the metabolism of the anticancer terpenoid indole alkaloids of Catharanthus roseus, has led to the need of establishing transient expression procedures for these two species. Therefore, the main goal of this work was the development and optimization of simple/fast, efficient and reproducible transient expression protocols for subsequent subcellular localization studies of proteins coded by Prx genes, and for characterization of candidate genes provided from omic approaches, namely implicated in the regulation, biosynthesis or transport of the valuable alkaloids from C. roseus. A complementary goal was to investigate the subcellular localization and sorting determinants of Prxs, namely the vacuolar sorting capacity of a C-terminal amino acid sequence extension (CTE) present in vacuolar Prxs, using as examples the well characterized and most abundant vacuolar Prx from C. roseus leaves, CrPrx1, and the most abundant Prx in the leaves of Arabidopsis, AtPrx34. For this, already available CrPrx1-GFP fusions and newly generated AtPrx34-GFP fusions were used in the transient expression assays. The successful establishment of protocols for PEG-mediated transformation of both Arabidopsis and C. roseus mesophyll protoplasts was achieved and validated as excellent transient expression systems. Transient expression by Agrobacterium infiltration of Arabidopsis and C. roseus leaves was also attempted, but it was only successful with in vitro C. roseus plants. However, promising insights were made into the development of this technique. Expression of CrPrx1-GFP fusions in Arabidopsis and C. roseus protoplasts using the established protocols confirmed the vacuolar localization of this Prx. Additionally the CrPrx1 signal peptide (SP) and CTE were confirmed as sorting determinants that target GFP to the ER and vacuole, respectively. The characterization of the subcellular localization and sorting determinants of AtPrx34 was not elucidated, possibly due to malfunctioning of the vector plasmid used for protoplast infiltration. In fact, upon agroinfiltration of in vitro C. roseus plants, it was possible to observe sorting to the ER of an SPAtPrx34-GFP fusion coded by a construct harboured in a binary vector plasmid, different from the one used for protoplast transformation. Thus, a resolution of the subcellular sorting of AtPrx34 should be possible in the near future. The transient expression assays described in the present study were highly reproducible, resulted in very satisfactory transformation efficiencies, and constitute a reliable and inexpensive methods that can be performed in most labs, and that are suitable test-systems to characterize genes of unknown function. This is also the the first time a transient expression system for C. roseus protoplasts is reported, using a PEGmediated transformation protocol.
Actualmente, a disponibilidade de inúmeras sequências genómicas exige o desenvolvimento de ferramentas para uma rápida caracterização ao nível protéico, onde de facto reside a função. Neste trabalho a caracterização de determinados genes de Peroxidases de Classe III (Prx) de Arabidopsis, assim como o interesse na caracterização de possíveis genes envolvidos no metabolismo de alcalóides indólicos terpenóides anticancerígenos de Catharanthus roseus, impulsionou a necessidade de estabelecer procedimentos de expressão transiente para estas duas espécies. Consequentemente, o objectivo principal deste trabalho foi o desenvolvimento e optimização de protocolos de expressão transiente simples/rápidos, eficientes e reproduzíveis para estudos de localização subcelular de proteínas codificados por genes Prx, e para a caracterização de possíveis genes obtidos de abordagens omicas, nomeadamente implicados na regulação, biossíntese ou transporte de alcalóides relevantes de C. roseus. Como objectivo complementar investigar a localização subcelular e sinais de direccionamento de Prxs, designadamente a capacidade de direccionamento vacuolar da extensão C-terminal aminoacídica (CTE) presente em Prxs vacuolares, utilizando como exemplos a Prx vacuolar mais abundante e estudada presente nas folhas de C. roseus, Crprx1, e a Prx mais abundante nas folhas de Arabidopsis, AtPrx34. Para tal, fusões CrPr1-GFP já disponíveis e fusões AtPrx34-GFP recém geradas foram utilizadas em procedimentos de expressão transiente. O estabelecimento com sucesso de protocolos de transformação mediada por PEG para protoplastos de mesófilo de Arabidopsis e C. roseus foi alcançado e validado como um excelente sistema de transformação transiente. Transformação transiente por infiltração com Agrobacterium de folhas de Arabidopsis e C. roseus foi abordado, mas apenas foram obtidos resultados positivos com plantas de C. roseus in vitro. Todavia progressos promissores foram realizados para o desenvolvimento desta técnica. Expressão de fusões CrPrx1-GFP em protoplastos de Arabidopsis e C. roseus utilizando os protocolos estabelecidos confirmaram a localização vacuolar desta Prx. Adicionalmente o péptido sinal (SP) e a extensão C-terminal (CTE) de CrPrx1 foram confirmados com sinais determinantes que direccionam a GFP para o RE e o vacúolo, respectivamente. A caracterização da localização subcelular e sinais de direccionamento de AtPrx34 não foram elucidados, possivelmente devido a uma irregularidade funcional do vector plasmídeal utilizado na transformação de protoplastos. De facto, após agroinfiltraçao de plantas C. roseus in vitro, foi possível observar o direccionamento para o RE da fusão SPAtPrx34-GFP codificada por um constructo incluído em vector binário, diferente do vector utilizado na transformação de protoplastos. Portanto, a caracterização do direccionamento subcelular da AtPrx34 poderá ser possível num futuro próximo. Os procedimentos de expressão transiente descritos no presente estudo manifestaram-se bastante reproduzíveis, resultando em níveis satisfatórios de eficiência de transformação, e constituem métodos fidedignos e de baixo custo que podem ser realizados na maioria dos laboratórios, e são sistemas-teste convenientes para caracterizar genes de função desconhecida. Foi também reportado pela primeira vez um sistema de transformação transiente em protoplastos de C. roseus, utilizando um protocolo de transformação mediada por PEG. Palavras-chave: Catharanthus roseus, Arabidopsis, transformação mediada por PEG, Agroinfiltração, fusões-sGFP, localização subcelular, via secretora, sinais de direccionamento, vacúolo, microscopia confocal.
Atnaseo, Chuthamat. "Transgenic Plant and Fungal Expression to Assay in vitro and in planta Activity of Sus scrofa beta-Defensin 1 and Nicotiana tabacum Defensin 1". Thesis, 2011. http://hdl.handle.net/10214/3193.
Texto completo da fontePeng, Hsien-Chieh, e 彭宣傑. "Study on the relatedness of LsGRP1 to the salicylic acid-induced resistance of Lilium cv. Star Gazer by agroinfiltration". Thesis, 2006. http://ndltd.ncl.edu.tw/handle/77579617912117147541.
Texto completo da fonte國立臺灣大學
植物病理與微生物學研究所
94
Lilium is a bulbous crop, severely affected by a major fungal disease, gray mold, caused by Botrytis elliptica. As know, development of necrotic lesions in the leaves of Oriental lily cultivar Star Gazer could be suppressed by the application of salicylic acid (SA). A cDNA sequence, named LsGRP1, coding for a 138-amino acid protein has been identified via suppression subtractive hybridization and differential screening. When the lily plants were treated with SA solution, the amount of LsGRP1 transcript analyzed by Northern blot hybridization increased. In the study of the relatedness between LsGRP1 and salicylic acid-induced disease resistance, agroinfiltration was applied for analysis of LsGRP1 transient expression in the leaves of Lilium cv. Star Gazer. In Northern blot hybridization, LsGRP1 transcript increased 72 hours after agroinfiltration of sense strand LsGRP1–expressing Agrobacterium tumefaciens EHA101 strain and more obviously 96 hours after agroinfiltraion. When the lily plants were treated with SA solution, the amount of LsGRP1 mRNA decreased in ‘Star Gazer’ leaves infiltrated with A. tumefaciens EHA101 strains expressing sense, anti-sense and hairpin LsGRP1, but the lesion numbers increased as compared to that in the control. These results showed that a gene silencing reaction was induced by introducing sense, anti-sense or hairpin LsGRP1, that abated the resistance of ‘Star Gazer’ leaves to gray mold disease. Thus, LsGRP1 playing an important role in salicylic acid-induced disease resistance of lily wasconcluded.
Capítulos de livros sobre o assunto "Agroinfiltratio"
Spiegel, Holger, Stefan Schillberg e Greta Nölke. "Production of Recombinant Proteins by Agrobacterium-Mediated Transient Expression". In Recombinant Proteins in Plants, 89–102. New York, NY: Springer US, 2022. http://dx.doi.org/10.1007/978-1-0716-2241-4_6.
Texto completo da fonteHelm, Jutta Maria, Elena Dadami e Kriton Kalantidis. "Local RNA Silencing Mediated by Agroinfiltration". In Methods in Molecular Biology, 97–108. Totowa, NJ: Humana Press, 2011. http://dx.doi.org/10.1007/978-1-61779-123-9_7.
Texto completo da fonteLiu, Lijing, Qingzhen Zhao e Qi Xie. "In Vivo Ubiquitination Assay by Agroinfiltration". In Methods in Molecular Biology, 153–62. Totowa, NJ: Humana Press, 2011. http://dx.doi.org/10.1007/978-1-61779-809-2_12.
Texto completo da fonteChen, Qiang, Matthew Dent, Jonathan Hurtado, Jake Stahnke, Alyssa McNulty, Kahlin Leuzinger e Huafang Lai. "Transient Protein Expression by Agroinfiltration in Lettuce". In Methods in Molecular Biology, 55–67. New York, NY: Springer New York, 2016. http://dx.doi.org/10.1007/978-1-4939-3289-4_4.
Texto completo da fonteD'Aoust, Marc-André, Pierre-Olivier Lavoie, Julie Belles-Isles, Nicole Bechtold, Michèle Martel e Louis-P. Vézina. "Transient Expression of Antibodies in Plants Using Syringe Agroinfiltration". In Recombinant Proteins From Plants, 41–50. Totowa, NJ: Humana Press, 2009. http://dx.doi.org/10.1007/978-1-59745-407-0_3.
Texto completo da fonteVon Lanken, Carol, e Arthur G. Hunt. "Transient Expression Using Agroinfiltration to Study Polyadenylation in Plants". In Methods in Molecular Biology, 127–33. New York, NY: Springer New York, 2014. http://dx.doi.org/10.1007/978-1-4939-2175-1_11.
Texto completo da fontePicard, Kelsey, Robyn Lee, Roger Hellens e Richard Macknight. "Transient Gene Expression in Medicago truncatula Leaves via Agroinfiltration". In Legume Genomics, 215–26. Totowa, NJ: Humana Press, 2013. http://dx.doi.org/10.1007/978-1-62703-613-9_15.
Texto completo da fonteMenassa, Rima, Adil Ahmad e Jussi J. Joensuu. "Transient Expression Using Agroinfiltration and Its Applications in Molecular Farming". In Molecular Farming in Plants: Recent Advances and Future Prospects, 183–98. Dordrecht: Springer Netherlands, 2011. http://dx.doi.org/10.1007/978-94-007-2217-0_9.
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