Artigos de revistas sobre o tema "601-700"

Neonatal mortality statistics are frequently reported in 100-g increments of birth weight. We tabulated our mortality statistics using two methods of incrementation: 500 to 599 g, 600 to 699 g, 700 to 799 g, etc. (method A) and 501 to 600 g, 601 to 700 g, 701 to 800 g, etc (method B). In each 100-g weight group, the mortality was less using method B. The average reduction in mortality using method B was 4.1%. Use of the two different methods creates difficulty in making meaningful comparisons of various published reports. We recommend that all future studies use method A, as that method is more consistent with previous recommendations of the World Health Organization.

Com o objetivo de avaliar a qualidade de silagens de sorgo, realizou-se o plantio, a colheita e a ensilagem de seis genótipos dessa forrageira utilizando-se delineamento inteiramente ao acaso com 18 tratamentos, sendo seis híbridos e três épocas de corte (leitoso, corte 1, pastoso, corte 2 e farináceo, corte 3) e quatro repetições. O material original foi analisado quanto ao teor de matéria seca (MS). Na abertura dos silos determinaram-se o pH e o N-NH3/NT no suco das silagens. As silagens foram analisadas quanto aos níveis de proteína bruta (PB), MS, frações fibrosas e teor de ácidos orgânicos. Os genótipos de sorgo avaliados foram: BR 601, BR 303, BR 304, AG 2006, BR 700 e BRS 701. Todos os híbridos apresentaram diferenças no teor de matéria seca no material original entre alguns cortes (P<0,05). Para essa característica, houve diferenças entre híbridos (P<0,05) para um mesmo corte. Quanto ao teor de MS da silagem, todos os híbridos apresentaram diferenças entre os cortes 1 e 2 e entre os cortes 1 e 3. Apenas os híbridos AG 2006 e BR 700 apresentaram diferenças entre os corte 2 e 3. Houve diferenças entre híbridos (P< 0,05) quanto ao teor de MS em um mesmo corte. O menor valor numérico para essa característica foi obtido no corte 1 do BR 601 (20,8%), e o maior valor foi para o BR 304 no corte 3 (49,1%). Os valores de pH do suco da silagem variaram entre 3,6 e 3,9. Os híbridos BR 303 e BR 304 apresentaram os maiores teores de PB. Conclui-se que os indicadores de padrões de fermentação (pH, N-NH3/NT, e teores de ácidos orgânicos) demonstram que as silagens em todos os tratamentos apresentaram bom padrão de fermentação final. De maneira geral, não houve alteração das frações fibrosas com o avanço do estádio de maturação. Quanto aos teores de matéria seca, todos os híbridos devem ser ensilados no estádio leitoso, exceto o BR 601, que deve ser ensilado no estádio de pastoso ou farináceo.

Para avaliar o efeito da presença de tanino sobre os parâmetros de degradabilidade in situ da matéria seca e da proteína bruta de seis genótipos de sorgo, ensilados no estádio de grão pastoso, foram utilizados quatro novilhos, canulados no rúmen, com peso vivo médio de 350kg, alimentados duas vezes ao dia com feno de Tifton 85. Os genótipos de silagem de sorgo utilizados foram: BR 303, BR 304, BR 601 e AG 2006 (sem tanino no grão) e BR 700 e BR 701 (com tanino no grão). Cinco gramas de cada amostra de silagem foram acondicionados em bolsas de náilon (15x8cm, 50mim) e suspensas no rúmen, utilizando os tempos de incubação de 6, 12, 24, 48, 72 e 96 horas. Foi calculada a fração solúvel no tempo zero (t0). Concluiu-se que o tanino no grão das silagens de sorgo testadas não afetou a média de desaparecimento com 96 horas de incubação. A presença de tanino em sorgos ensilados no estádio de grão pastoso foi capaz de inibir somente a degradabilidade potencial da MS. Não houve efeito negativo do tanino para as silagens de sorgo BR 700 e BR 701 sobre os parâmetros de degradabilidade potencial e taxa de degradação da proteína bruta.

Carbon electrodes have been successfully synthesized and characterized for desalination system based on Capacitive Deionization (CDI) technology. The carbon electrodes were synthesized with an activated carbon (700 – 1400 m2/g), carbon black, glutaric acid and polyvinyl alcohol as a binder using cross-linking method at 120 °C. The study was to investigate salt removal percentage value of 0.1 M NaCl, MgCl and KCl using CDI cell. A solution with 0.1 M was supplied to the cell using a Boyu Submersible pump (model SP-601) at a flow rate of 10 mL/min. When potentials of 1.0, 2.0, and 3.0 V were applied to the CDI cell, the result showed that a solution of 0.1 M KCl has greater salt-removal percentage than the solutions with 0.1 M NaCl and MgCl. The salt-removal percentage of KCl was achieved at 55% for 3.0 V whilst resulted in 20 and 30% for NaCl and MgCl, respectively.

This study aimed to evaluate in situ degradability and degradation kinetics of DM, NDF and ADF of silage, with or without tannin in the grains. Two isogenic lines of grain sorghum (CMS-XS 114 with tannin and CMS-XS 165 without tannin) and two sorghum hybrids (BR-700 dual purpose with tannin and BR-601 forage without tannin) were ensiled; dried and ground silage samples were placed in nylon bags and introduced through the fistulas. After incubation for 6, 12, 24, 48, 72 and 96 hours, bags were taken for subsequent analysis of fibrous fractions. The experimental design was completely randomized with 4 replicates and 4 treatments and means compared by Tukey’s test at 5% probability. As for the DM degradation rate, silage of CMSXS165without tannin was superior. Silages of genotypes BR700 and CMSXS 114 with tannin showed the highest values of indigestible ADF (59.54 and 43.09%). Regarding the NDF, the potential degradation of silage of CMSXS165 line without tannin was superior. Tannin can reduce ruminal degradability of the dry matter and fibrous fractions.

Abstract Background In patients with Ph-negative MPN, a prior thrombosis (PrTh) occurs in around 1/5 of cases, with variable platelet (PLT) count and variable distance from diagnosis. Objective To investigate the influence of PLT count at PrTh on diagnostic and therapeutic approach in MPN patients. Material and methods We evaluated 129 MPN patients from RIT, reclassified according to WHO 2008 criteria as ET (n70), initial-primary myelofibrosis (n29), early-PV (n10), and unclassifiable-MPN (n20). Results Patients, 60 males and 69 females, showed following PrTh: 91(71%) major arterial (37 AMI, 4 angina, 24 stroke, and 26 TIA); 12(9%) minor arterial; 22(17%) major venous (8 DVT, 7 splanchnic, 4 cerebral sinus, 3 pulmonary embolism); and 4(3%) minor venous events. PrTh occurred at a median distance of 4.1 months (range 0.1-118) from MPN diagnosis. This distance was >24 months in 21(16%) patients. At occurrence of PrTh, median age was 58 years. PLT count (x109/L) had a median value of 661 (range 150-2200), and was ≤450, 451-600, 601-700, 701-1000, and >1000 in 15(12%), 35(27%), 26(20%), 43(33%), and 10(8%) patients, respectively. Median white blood cell (WBC) count was 9.0 x 109/L and median hematocrit (HCT) value was 46% in males, and 41% in females. Median time (months) from PrTh to diagnosis of MPN was higher (p0.004) in patients with lower PLT count (x 109/L): ≤450 (50.2), 451-600 (11.7), 601-700 (2.7), 701-1000 (1.8), and >1000 (1.4). After occurrence of PrTh, all patients received conventional anti-thrombotic treatment, but in 7(5.4%) patients 9 recurrent thrombosis were reported before MPN diagnosis (11/100 pt-years). At MPN diagnosis, clonality was documented in 101(78%) patients (JAK2 V617F mutation in 96 cases, 74%). The age was >60 years in 61(47%) patients. PLT count (x109/L) had a median value of 720 (166-2440), and was ≤450 (n 7, 5%), 451-600 (n 21, 16%), 601-700 (n 28, 22%), 701-1000 (n 58, 45%), >1000 (n15, 12%). WBC count (109/L) had a median value of 8.9, and was >10 in 40 (31%) cases. Median HCT level (%) was 45.6 in males and 42.1 in females. Cardiovascular risk factors (CVRF), comorbidities and symptoms were documented in 103(80%), 97(75%), and 57(44%) cases, respectively. Thrombotic risk (IPSET-Th) was high in 97.5%, and intermediate in 2.5% of cases. All 129 patients received anti-thrombotic drugs (low dose aspirin in 95% of cases) and, immediately after the diagnosis, they started a cytoreductive treatment (hydroxycarbamide 89%, anagrelide 8%, interferon-alpha 3%). Patients with a PLT count (x109/L) at PrTh ≤600(n 50), as compared with those with a PLT count >600(n 79), showed a longer median time to the MPN diagnosis (16.7 vs 2.0 months, p<0.001). No significant difference was found in the rate of: arterial PrTh (80% vs 79.7%, p0.97); recurrence of thrombosis before the diagnosis (8% vs 4%, p0.69); JAK2 V617F mutation (80% vs 71%, p0.29); age >60 years (52% vs 44%, p0.39); CVRF (82%vs79%, p0.63); WBC >10 x109/L (23% vs 39%, p0.07); HCT high level [>47% in males, >44% in females](28% vs 36%, p0.37), and high thrombotic risk [IPSET-Th] (96%vs99%, p0.56). During follow-up (median 7.9 years) they showed a higher incidence of thrombosis recurrence (30%vs15%, p0.04; 4.5 vs 1.7/100 pt-y, p<0.01) Conclusion Time to MPN diagnosis was significantly longer in patients with PLT count (x109/L) at PrTh ≤600 vs >600, and this time to diagnosis was characterized by a not negligible thrombosis recurrence. Moreover, during follow-up they showed a higher incidence of thrombosis recurrence. This analysis strongly suggests that a PLT count <600 or even <450 x109/L, in patients with unexplained thrombosis, deserves the search of a probable MPN, in order to promptly start cytoreductive treatment in addition to a conventional anti-thrombotic therapy. Disclosures Passamonti: Novartis: Consultancy, Honoraria, Speakers Bureau. Vannucchi:Novartis: Membership on an entity's Board of Directors or advisory committees, Speakers Bureau; Novartis: Other: Research Funding paid to institution (University of Florence), Research Funding; Shire: Speakers Bureau; Baxalta: Membership on an entity's Board of Directors or advisory committees.

Objectives: The objective of the paper is to discuss the proposed dynamic software model to detect and prevent intrusion in the cloud network. Methods: The Behavior Profiling Algorithm (BPA) has been used to detect the intrusion in cloud network. For finding the intruder in the network the Event Log Entries and the network Unique Identification Address (UIA) has been fetched from the server and then the collected attribute values have been transferred to prevention module. In the prevention module the dynamic statistical approach model has been used to prevent the network systems and data which are available in the Cloud Network. Findings: For testing the proposed model the 100 cloud network systems were taken and based on the loss of packets (in MB) ranges the samples were classified as 0-100, 101-200, 201-300, 301-400, 401-500, 501-600, 601-700 respectively. The range of data loss is assumed to be an interval of 100 Mbps. It is assumed that the higher the data loss ranges, the more data is lost. The mean, variance, and standard deviation were calculated to verify the data loss ranges. The mean (average) of the data loss in the ranges 0-100 is 060.77 and the mean in the ranges 101-200 is 144.714 data losses, which gradually increases in proportion to the data loss ranges, and in the ranges 601-700 it is 665.769 data losses. From the statistical approach model, the differences between mean and variance indicated that the intruder attacked the files during the data transformation in the network. Therefore, the administrator has to monitor the warning message from the proposed IPS model and get data packet losses in the transformation. If the frequency of data loss is low, the administrator can assume that the data flow is low due to network problems. On the other hand, if the frequency of data loss in the network system is high, he can block the transformation and protect the data file. This paper concludes that the behavioral profiling algorithm combined with a statistical model achieves an efficiency of over 96% in wired networks, over 97.6% in wireless networks, and over 98.7% in cloud networks. Novelty: In the previous paper discussed the approach which has been implemented with 40 nodes and the result of the proposed algorithm produced above 90%, 96% and 98% in the wired, wireless and cloud network respectively. Now, the model has been implemented with 100 nodes the result has been increased. This study concluded that, the efficient algorithm to detect the intrusion is behaviour profiling algorithm, while join with the statistical approach model, it produces efficient result.

O conhecimento da proporção da matéria-prima que será transformada em produtos finais para comercialização, bem como da quantidade que fará parte do resíduo do processamento permite o planejamento logístico da produção e os cálculos necessários para a avaliação da eficiência produtiva da empresa processadora de pescado. O jundiá Rhamdia quelen é um bagre nativo da América do Sul e que vem sendo estudado há alguns anos por vários grupos de pesquisa latino-americanos, apresentando resultados que atestam seu potencial para a piscicultura. O objetivo do presente trabalho foi avaliar o rendimento do jundiá como matéria-prima para o processamento mínimo em diferentes classes de pesos, a saber: 200 – 300 g, 301 – 400 g, 401 – 500 g, 501 – 600 g, 601 – 700 g, 701 – 800 g. Devido ao crescimento mais acelerado das fêmeas, apenas indivíduos do sexo feminino foram encontrados nas classes de peso acima de 500 g. O rendimento de filé foi maior para os peixes com maior peso médio (728g - 34,7%). A porcentagem de músculo abdominal esteve entre 9,3% e 11,2%. A proporção de vísceras sem as gônadas encontrou-se entre 7,8 e 9,8% e a porcentagem de gônadas das fêmeas com peso médio 572 g representou 11,1% do peso total. A descrição da composição da carcaça dos peixes cultivados tem grande importância sob o aspecto econômico e produtivo, permitindo estimativas de produtividade tanto para o piscicultor quanto para a indústria de processamento.

Abstract Individuals with schizophrenia have a reduced life-expectancy compared to the general population, largely due to an increased risk of cardiovascular disease (CVD). Clinical and epidemiological studies have been unable to unravel the nature of this relationship. We obtained summary-data of genome-wide-association studies of schizophrenia (N = 130 644), heart failure (N = 977 323), coronary artery disease (N = 332 477), systolic and diastolic blood pressure (N = 757 601), heart rate variability (N = 46 952), QT interval (N = 103 331), early repolarization and dilated cardiomyopathy ECG patterns (N = 63 700). We computed genetic correlations and conducted bi-directional Mendelian randomization (MR) to assess causality. With multivariable MR, we investigated whether causal effects were mediated by smoking, body mass index, physical activity, lipid levels, or type 2 diabetes. Genetic correlations between schizophrenia and CVD were close to zero (−0.02–0.04). There was evidence that liability to schizophrenia causally increases heart failure risk. This effect remained consistent with multivariable MR. There was also evidence that liability to schizophrenia increases early repolarization pattern, largely mediated by BMI and lipids. Finally, there was evidence that liability to schizophrenia increases heart rate variability, a direction of effect contrasting clinical studies. There was weak evidence that higher systolic blood pressure increases schizophrenia risk. Our finding that liability to schizophrenia increases heart failure is consistent with the notion that schizophrenia involves a systemic dysregulation of the body with detrimental effects on the heart. To decrease cardiovascular mortality among individuals with schizophrenia, priority should lie with optimal treatment in early stages of psychosis.

O objetivo do estudo foi descrever a morfologia e a composição centesimal da pele de tilápia do Nilo e as alterações que ocorrem ao longo do processo de curtimento. Foram retiradas amostras (região dorsal média) no término de cada etapa do processo de curtimento, para análises de microscopia de luz e microscopia eletrônica de varredura. Também foram realizadas análises da composição centesimal de peles referentes a peixes de três classes de peso (500 a 600g; 601 a 700 g e 701 a 800 g). Observou-se que a pele da tilápia do Nilo possui um padrão estrutural comum aos peixes teleósteos. Através de técnicas histoquímicas comprovou-se a presença de carboidratos neutros nas células mucosas encontradas na camada superficial da epiderme. Foi observado o arranjo e orientação das fibras colágenas da derme superficial, bem como da derme compacta e a organização das fibras colágenas e das lámelulas de proteção na inserção das escamas. Pode-se observar a inserção da escama no tecido dérmico, assim como, a sua morfologia. Observou-se o intumescimento e abertura da estrutura fibrosa no início do processo, e principalmente, a separação das camadas de fibras colágenas ao longo do processo e a ação dos óleos do engraxe ao término do curtimento. Houve diferença na composição centesimal das peles, apenas quanto ao teor de lipídios. Pela análise da morfologia da pele, em relação à organização e disposição das fibras colágenas e da estrutura dérmica durante o curtimento, pode-se inferir que o estudo da estrutura histológica do material é fundamental para análise da resistência do couro.

The Trans Texas Video Conference Network (TTVN) has been linked to all Texas A&M Univ. campuses and most of the Regional Research and Extension Centers. The College of Agriculture and Life Sciences has funded an aggressive project of establishing TTVN class rooms in many departments across the College Station campus, including The Horticultural Science Dept. in 1997. The first two Hort courses taught were HORT 422 Citrus and Subtropical Fruits in Fall 1996 and HORT 418 Nut Culture in Spring 1997. This extended the class room 400 miles south to Weslaco, 300 miles north to Texarkana and Dallas, and 700 miles west to El Paso. Students at each site had video and audio interaction with the professor and with each other. Advantages included the availability of college credit courses to areas where this subject matter did not previously exist, which helps fulfill the Land-grant University Mission. Quality was maintained through lecture and lab outlines on Aggie Horticulture, the department's Web home page, term papers written to ASHS serial publicationspecifications, and rigorous examinations monitored by site facilitators. Lecture presentations were presented via Power Point, which took about twice as long to prepare than traditional overhead transparencies. Administrative problems remain, but will be solved when the requested Distance Education Registration Category is initiated so that subvention credit can be shared. The lecture portion of the graduate course, HORT 601 Nutrition of Horticultural Plants, will be taught in the fall semester 1997 at eight sites throughout the state.

This study of mosquitoes in Lika, geographically, geologically and climatically a very specific region, was conducted to gain insight into the composition and relative abundance of the mosquito fauna, as well as to check for the possible presence of invasive species. Sampling took place from July to September 2020. Mosquito larvae were captured from medium and small breeding sites using a 25 cm diameter net and a plastic dipper. Adult specimens were sampled in both urban and rural areas using CO2 baited CDC traps, CO2 baited BG Sentinel traps with BG Lure and human landing catch. The study was conducted at 69 sites. We collected 5,126 specimens (100 adults and 5,026 larvae) and demonstrated the presence of 16 mosquito species. CDC traps were almost five times more effective (quantity and quality) than BG Sentinel traps. The most common mosquito taxa caught in Lika were Culex pipiens complex (78.36% of all larvae) and Ae. geniculatus (30.26% of all adults). Both invasive mosquito species present in Croatia, Aedes albopictus and Ae. japonicus, were recorded, with Ae. albopictus being detected in Lika for the first time. Aedes japonicus was widespread, recorded in 23 localities. According to molecular analysis, only An. maculipennis s.s. was confirmed in the An. maculipennis complex. Within the Cx. pipiens complex, both Cx. pipiens biotypes, pipiens and molestus were confirmed together with one single hybrid specimen. Analysis of mosquito occurrence with regard to altitude shows that most samples and species were collected between 601 and 700 m a.s.l., although the Shannon evenness index and Hill’s index show the highest value in the range of 901 to 1140 m a.s.l. In this systematic study on the mosquito fauna in Lika, a significant species diversity, including invasive species, was found.

Este estudo teve como objetivo avaliar a resistência do couro da tilápia do Nilo em função de classes de peso corporal de abate e sentidos (longitudinal e transversal) do couro em relação ao comprimento do corpo do peixe. As peles foram classificadas em categorias de peso corporal diferentes (C1= 500 a 600 g; C2 = 601 a 700 g e C3 = 701 a 800 g) e congeladas para posterior curtimento. Após descongelamento, as peles foram curtidas com sais de cromo e submetidas ao recurtimento com tanino vegetal. Depois, obtiveram-se os corpos-de-prova, que foram analisados quanto a espessura para realização dos testes de resistência à tração e ao alongamento e rasgamento progressivo, utilizando-se o dinamômetro EMIC. Através da análise de regressão linear, constatou-se que existe uma correlação entre a área da pele (Y) e peso do peixe (X), que pode ser expressa pela equação linear Y=39,359 + 0,0269X (r=0,56**). Houve efeito da classe de peso do peixe sobre as cargas de força aplicadas nos testes de resistência e no sentido transversal do couro com maior resistência a tração e rasgamento progressivo comparada ao longitudinal. O conteúdo de óxido de cromo presente nos couros variou de 3,75 a 3,81%, enquanto o de substâncias extraíveis em diclorometano variou de 8,16% a 8,47%, e pH ficou entre 3,84 a 3,86 para as categorias de peso. As peles curtidas de tilápia pesando entre 500 a 800 g não diferiram na resistência, quanto a tração, alongamento e rasgamento progressivo. No entanto, quando avaliado o sentido de retirada dos corpos-de-prova, o sentido transversal (referente a largura do couro) apresentou maior resistência quanto à tração e rasgamento progressivo quando comparado ao longitudinal (comprimento do couro).

207 Background: In patients previously treated for aHCC, cabozantinib (cabo) led to longer overall survival and progression-free survival vs placebo (pbo) in the randomized, phase 3 CELESTIAL trial (NCT01908426; N = 707). CELESTIAL was stopped early for benefit at the second interim analysis. This post hoc analysis estimated the incremental quality-adjusted life years (QALYs) accrued in CELESTIAL. Methods: Health utility was elicited at each study visit using the EQ-5D-5L quality of life questionnaire. (completed by 82–100% of patients overall). UK crosswalk tariffs were applied for health states. Cumulative QALYs by patient were calculated by linear interpolation; for patients who were censored (31% of patients; including 9% within 100 days of randomization), the last observed utility value was carried forward to study end. The difference in restricted mean QALYs was calculated using generalized linear models, accounting for baseline utility, and with 0.06–0.08 QALYs considered the minimal important difference. Results: At day 50 after randomization (acute treatment phase), cabo was associated with a small reduction in mean total QALYs vs pbo (difference −0.003; 95% CI −0.005 to −0.002; p ≤ 0.001; n = 601 [cabo, n = 389; pbo, n = 212]). At day 100, there was a numerical benefit in mean total QALYs for cabo (difference +0.007; 95% CI −0.001 to 0.015; p = 0.103; n = 627 [cabo, n = 410; pbo, n = 217]), and at day 150 the difference was +0.032 QALYs (95% CI 0.017 to 0.047; p ≤ 0.001; n = 629 [cabo, n = 412; pbo, n = 217]) in favor of cabo. Over the entire follow-up, patients randomized to cabo accrued a mean of +0.092 (95% CI 0.016 to 0.169; p = 0.018; n = 700 [cabo, n = 465; pbo, n = 235]) additional QALYs compared with those receiving pbo. Using alternative Devlin weights for health states, the mean accrued QALYs with cabo was +0.115 vs pbo (95% CI 0.032 to 0.198; p = 0.007). Conclusions: Cabo was associated with an initial, small reduction in health utility. However, with continued treatment, health utility increased and at the end of the study there was a clinically and statistically significant benefit in mean QALYs in favor of cabo. Clinical trial information: NCT01908426.

PURPOSE: To determine the maximum-tolerated dose (MTD), dose-limiting toxicities, and efficacy of gemcitabine combined with fluorouracil (5-FU) in patients with pancreatic cancer. PATIENTS AND METHODS: Patients with measurable, locally advanced, nonresectable or metastatic pancreatic cancer were candidates for the study. 5-FU was given via protracted venous infusion (PVI) at a fixed dosage of 200 mg/m2/d, and gemcitabine was administered weekly for 3 consecutive weeks every 4 weeks. The initial dose of gemcitabine was 700 mg/m2 and was escalated in increments of 100 mg/m2/wk until the appearance of severe toxicity. Measurements of efficacy included the following: response rate; clinical benefit response, which is a composite measurement of pain, performance status, and weight loss; time to disease progression; and survival. RESULTS: Twenty-six patients received a total of 109 courses. Dose-limiting toxicity, which consisted of grade 4 neutropenia with fever (one patient) and grade 4 thrombocytopenia (one patient), was observed in two of three patients treated with 1,100 mg/m2/wk of gemcitabine. On the basis of these results, the MTD of gemcitabine with 5-FU via PVI on this schedule was 1,000 mg/m2. Sixteen patients developed grade 3-4 neutropenia, and three patients developed grade 3-4 thrombocytopenia. Grade 3-4 nonhematologic toxicity consisted of diarrhea (two patients) and cutaneous toxicity, asthenia, edema, mucositis, and nausea and vomiting (one patient each). The delivered dose-intensity of gemcitabine was similar at the 1,000 mg/m2 dose level (599 mg/m2/wk) as at the 900 mg/m2 (601 mg/m2/wk) dose level. For this reason, the recommended dose of gemcitabine for phase II evaluation on this schedule was 900 mg/m2. Five patients had objective responses (one complete response and four partial responses; response rate, 19.2%; 95% confidence interval [CI], 6.5 to 39.3), and 10 patients had improvement of disease-related symptoms (45%; 95% CI, 24 to 67). After a median follow-up of 17.7 months (range, 7.8 to 24.8 months), the median progression-free survival and overall survival times were 7.4 months (95% CI, 3.3 to 11.4) and 10.3 months (95% CI, 8.1 to 12.5), respectively. CONCLUSION: The MTD of gemcitabine when combined with 5-FU via PVI on this schedule was 1,000 mg/m2/wk; however, on the basis of administered dose-intensity, the recommended dose for additional investigation is 900 mg/m2. This combination chemotherapy regimen was well tolerated and showed promising antitumor activity in the treatment of pancreatic cancer.

Abstract Abstract 339 Initial reports that high dose imatinib results in better responses more rapidly than standard dose imatinib remain controversial. The German CML Study Group therefore compared imatinib 800 mg (IM 800) with standard dose imatinib +/- IFN (IM 400, IM 400 + IFN) in newly diagnosed, not pretreated CML with regard to molecular response at 12 months and survival in a randomized clinical trial. By April 30, 2009, 1026 chronic phase CML patients have been randomized (326 for IM 400, 338 for IM 800, 351 for imatinib + IFN). Comparison was for molecular and cytogenetic remissions, overall (OS) and progression free (PFS) survival and toxicity. 1015 patients were evaluable at baseline, 904 for survival analysis (294 for IM 400, 286 for IM 800, 324 for IM 400+IFN), 790 for cytogenetic (analysis of at least 20 metaphases required) and 823 for molecular response. The three treatment groups were similar regarding median age, sex, median values of Hb, WBC, platelets and distribution according to the EURO score. Median follow-up was 25 months in the imatinib 800 mg arm and 42 months in the imatinib 400 mg +/-IFN arms. The difference is due to the fact that at first the IM 800 arm was designed for high risk patients only and opened up to all risk groups in July 2005. The median daily doses of imatinib were 626 mg (209- 800 mg) in the IM 800 arm and 400 mg (184- 720 mg) in the IM 400 +/- IFN arms. Of 218 patients receiving imatinib 800 mg and evaluable for dosage at 12 months, 100 (45.9%) received more than 700 mg/day, 27 (12.4%) 601-700 mg, 37 (17.0%) 501-600 mg, 48 (22.0%) 401-500 mg and only 6 (2.8%) 400 mg/day or less. The cumulative incidences at 12 months of complete cytogenetic remission (CCR) were 52.3%, 64.9% and 50.6%, and of major molecular remission (MMR) 30.2%, 54.3% and 34.6% with IM 400, IM 800 and IM 400 +IFN, respectively. The cumulative incidences of achieving CCR and MMR with IM 400, IM 800 and IM 400+IFN at 6, 12, 18 and 24 months after start of treatment are summarized in the table. MMR at 12 months was reached faster with IM 800 than with IM 400 (p=0.0003) or IM400+IFN (p=0.0131). Optimal molecular response (OMR=<0.01% BCR-ABL according to the international scale) was reached with IM 800 after a median of 31.3 months vs. 47.5 and 42.5 months with IM 400 +/- IFN. Also CCR was reached faster with IM 800 (p<0.01). The more rapid achievement of MMR with IM 800 was observed in low and intermediate risk patients with little or no difference in high risk patients. In an analysis “as treated” patients receiving more than 600 mg/day reached remissions faster than those receiving lower dosages (CCR after a median of 7.8 vs. 8.9 months, MMR after a median of 10.4 vs. 12.9 months). At the time of this evaluation, OS (92% at 5 years) and PFS (88% at 5 years) showed no difference. Type and severity of adverse events (AE) at 12 months did not differ from those expected (all grades and grades III/IV). Hematologic (thrombocytopenia 7% vs. 4%) and non-hematologic AEs (gastrointestinal 35% vs. 15-24% and edema 29% vs. 16-19%) were more frequent with IM 800, fatigue (14% vs. 7-13%) and neurological problems (15% vs. 6-7%) more frequent with IM 400 + IFN (all grades). These data show a significantly faster achievement of MMR at 12 months with IM 800 as compared to IM 400 +/-IFN. So far, this faster response rate did not translate into better OS or PFS. Hence IM 400 should still be considered as standard of care. With some individual dose adjustments tolerability of IM 800 was good. Longer observation is required to determine whether this more rapid achievement of MMR and CCR will have a long term impact or not. Disclosures: German CML Study Group: Deutsche Krebshilfe: Research Funding; Novartis: Research Funding; European LeukemiaNet: Research Funding; Kompetenznetz Leukämie: Research Funding; Roche: Research Funding; Essex: Research Funding.

Background MB66 film is a multipurpose prevention technology (MPT) product with monoclonal antibodies (mAbs) against HIV-1 (VRC01-N) and HSV-1 and 2 (HSV8-N). The mAbs were produced by transient expression in Nicotiana benthamiana (N). We conducted a Phase I clinical trial to assess the safety, pharmacokinetics (PK), and ex vivo efficacy of single and repeated doses of MB66 when used intravaginally. Methods and findings The clinical trial enrolled healthy reproductive-aged, sexually abstinent women. In Segment A, 9 women received a single MB66 film which was inserted into the vaginal posterior fornix by a clinician. In Segment B, 29 women were randomly assigned to MB66 (Active) or Placebo film groups and were instructed to insert 1 film vaginally for 7 consecutive days. Visits and clinical sampling occurred predose and at various time points after single and repeated film doses. The primary endpoint was number of adverse events (AEs) Grade 2 or higher related to product use. Secondary endpoints included film dissolution rate, Nugent score (a Gram stain scoring system to diagnose bacterial vaginosis), vaginal pH, post-use survey results, cytokine concentrations in cervicovaginal lavage (CVL) specimens (assessed by Luminex assay), mAb concentrations in vaginal fluid collected from 4 sites (assessed by ELISA), and HIV and HSV neutralization activity of CVL samples ex vivo (assessed by TZM-bl and plaque reduction assay, respectively). The product was generally safe and well tolerated, with no serious AEs recorded in either segment. The AEs in this study were primarily genitourinary in nature with the most commonly reported AE being asymptomatic microscopic hematuria. There were no differences in vaginal pH or Nugent scores or significant increases in levels of proinflammatory cytokines for up to 7 days after film insertion in either segment or between Active and Placebo groups. Acceptability and willingness to use the product were judged to be high by post-use surveys. Concentrations of VRC01-N and HSV8-N in vaginal secretions were assessed over time to generate pharmacokinetic curves. Antibody levels peaked 1 hour postdosing with Active film (median: 35 μg/mL) and remained significantly elevated at 24 hours post first and seventh film (median: 1.8 μg/mL). Correcting for sample dilution (1:20), VRC01-N concentrations ranged from 36 to 700 μg/mL at the 24-hour time point, greater than 100-fold the IC50 for VRC01 (0.32 μg/mL); HSV8-N concentrations ranged from 80 to 601 μg/mL, well above the IC50 of 0.1 μg/m. CVL samples collected 24 hours after MB66 insertion significantly neutralized both HIV-1 and HSV-2 ex vivo. Study limitations include the small size of the study cohort, and the fact that no samples were collected between 24 hours and 7 days for pharmacokinetic evaluation. Conclusions Single and repeated intravaginal applications of MB66 film were safe, well tolerated, and acceptable. Concentrations and ex vivo bioactivity of both mAbs in vaginal secretions were significantly elevated and thus could provide protection for at least 24 hours postdose. However, further research is needed to evaluate the efficacy of MB66 film in women at risk for HIV and HSV infection. Additional antibodies could be added to this platform to provide protection against other sexually transmitted infections (STIs) and contraception. Trial registration ClinicalTrials.gov NCT02579083.

The work under review is the first volume of the catalogue of ancient Uyghur manuscripts and xylographs preserved in the Institute of Oriental manuscripts of the Russian Academy of Sciences (St Petersburg). This is the largest collection of texts and documents transcribed in the ancient Uyghur language in Russia. The catalogue is subdivided into 12 thematic sections. They comprise descriptions of 564 ancient Uyghur fragments, altogether 601 entries. The publication is accompanied by three prefaces, extensive scholarly introductions in Russian and English languages, as well as indexes and facsimiles of most documents.

The debate about diagnoses and treatment of attention deficit hyperactive disorder (ADHD) in children continue to range on between the developmental and biological perspectives. While there is increasing evidence that support the biological susceptibility of the disorder, a number of researches also emphasized the significant effect of environment on the syndrome. This study used developmental perspectives to evaluate and bring together various bio-psychosocial factors that impact on children diagnosed with ADHD. The study explored and integrated the existing and advancing study on ADHD to a more refined pattern that embraced developmental perspectives. The study also discussed how the linkage in childhood ADHD fits within the developmental psychopathology perspective. 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El objeto de este trabajo es el estudio del simbolismo de las aves en el ámbito tartésico y fenicio-púnico en la península ibérica durante el Bronce Final y el Hierro I. Se han recogido y analizado aquellas piezas con representaciones de aves, así como los restos orgánicos de éstas, si bien esto último no ha dado muchos frutos debido a las dificultades que existen tanto para su conservación como para la posterior identificación de especies. En total se han podido determinar ánades, gallos, palomas, flamencos, cisnes, lechuzas y halcones, todas ellas representadas en el Mediterráneo oriental y cuya iconografía se vincula al mundo funerario, al tránsito al Más Allá y a las divinidades. Gran parte de esa iconografía llegó a la península de mano de los fenicios, si bien su acogida y aceptación entre la población local fue variable. Palabras clave: aves, simbolismo, tartesios, fenicios, púnicosTopónimos: península ibéricaPeriodo: Hierro I. ABSTRACTThe aim of this paper is to study the symbolism of birds in Tartessian and Phoenician-Punic cultures within the Iberian Peninsula during the late Bronze and early Iron Age. To this end, items with any sort of symbolism connected with birds have been analysed. Organic remains have also been examined, although the latter did not make a relevant contribution to the study due to problems of conservation of the organic remains and subsequent identification of species. I have identified ducks, roosters, pigeons, flamingos, swans, owls and hawks, all located around the East Mediterranean basin and related to funerary contexts, the journey to the hereafter and deities. Most of this iconography reached the Iberian Peninsula via Phoenician culture, albeit its acceptance among the local population varied. Keywords: birds, symbolism, Tartesian, Phoenicians, PunicPlace names: Iberian PeninsulaPeriod: Iron Age REFERENCIASAlmagro Gorbea, M. J. 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La Transición entre el Bronce Final y la Primera Edad del Hierro en el valle medio del Guadiana”, en Territorios comparados: Los valles del Guadalquivir, el Tajo y el Guadiana en época tartésica (Anejos de AEspA, 80), Mérida, pp. 183-212.Coldstream, J. N. (2003), Geometric Greece, 900-700 BC, London-New York.Corzo, R. (1988), Los fenicios, señores del mar. Historias del Viejo Mundo (Historia 16, 8), Madrid.— (1991): Arte fenicio y púnico. Cuadernos de arte español (Historia 16, 9), Madrid.De Deus, M. y Correira, J. (2005), “Corte Margarida. Mais uma necrópole orientalizante no Baixo Alentejo”, en El Periodo Orientalizante. Actas del III Simposio Internacional de Arqueología de Mérida: Protohistoria del Mediterráneo Occidental, Mérida, pp. 615-618.Díez, F. (2017), “Cuerpos imaginarios: poder y descorporeización en el paso al más allá imaginado en las lécitos áticas de fondo blanco”, Res Publica, 20.3, pp. 493-506.Diogo, C. y Kesser, C. 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A new fluorimetric and colorimetric dual-mode probe, 4-bromo-2-(hydrazonomethyl) phenol (BHP) has been synthesized and successfully utilized for the recognition of Cd2+/F‒ ions in DMSO/H2O (9:1, v/v) system. The probe displays dual channel of detection via fluorescence enhancement and colorimetric changes upon binding with F‒ and Cd2+ ions respectively. The Job’s plot analysis, ESI-MS studies, Density Functional Theoretical (DFT) calculations, 1H NMR and 19F NMR titration results were confirmed and highly supported the 1:1 binding stoichiometry of the probe was complexed with Cd2+/F‒ ions. Furthermore, intracellular detection of F‒ ions in HeLa cells and fluorescence imaging analysis in Zebrafish embryos results of the probe BHP might be used to reveal their potential applications in a biological living system. Introduction The quantification and detection of toxic metal ions in diverse fields have fascinated more attention in recent years due to their prominent and significant roles in clinical diagnosis and ecological system.1–6 Besides metal ions, anions also play an exclusive role in a variety of chemical and biological processes.7–12 In earlier, analytical methods for the detection of cations/anions has required highly sophisticated and expensive instruments such as atomic absorption spectrometry, inductively coupled plasma mass spectrometry, ion sensitive electrodes, and gas and ion chromatography. Amid, fluorescent techniques have more expedient in terms of rapidness, excellent sensitivity and selectivity, low cost, easy and feasible detection. In addition, optical detection mode analysis is a more appropriate method because of their potential features such as easy handling, real-time analysis and different signal output modes.13–16 Besides, colorimetric assays are more feasible and potent tool as they provide a simple visible authentication for analyte detection in the absence of instruments and tedious techniques. In this perspective, the recent research area has been mainly focused to design the novel multi-functional fluorometric and colorimetric sensors for the detection of ions in the different environments. Cadmium (Cd2+) is one of the important hazardous heavy transition metal ions17 in the environment due its carcinogenic nature. The higher accumulation of Cd2+ ion and inhalation of Cd-dust prompts more awful health issues in human like cancer, cardiovascular diseases, kidneys and liver damage.18 Furthermore, the Cd2+ ion has more advantages in several industries such as pigments in plastics, electroplating and batteries, etc. On the other hand, fluoride ions play an ample role in dental health and in the treatment of osteoporosis.19–22 The excess of fluoride ingestion prompted severe disease in human health like gastric and kidney problems.23 In some remote areas, the high level contamination of fluoride ions in drinking water triggered bone disease such as fluorosis.24–31 Thus, to develop and synthesize novel multifunctional probe for the detection and quantification of both cations and anions is a highly anticipated and imperative task. Scheme 1. Synthesis of probe BHP Herein, we have fabricated and synthesized a novel chromogenic and fluorogenic assay based on bromo substituted salicylhydrazone moiety for the colorimetric and fluorometric detection of F‒ ions and colorimetric detection of Cd2+ ions in DMSO/H2O (9:1, v/v) system. The UV-visible and fluorescence spectral analysis of BHP with Cd2+/F‒ ions exposed an outstanding ratiometric absorbance and colorimetric responses towards F‒ ions and also showed a visible colorimetric response towards Cd2+ ions. The fluorescence enhancement of BHP with F‒ ion was highly evaluated by DFT calculations. As well, the cell viability experimental results of BHP can be used for the detection of F‒ ions in both HeLa cells and Zebrafish embryos via high content analysis system. Experimental Methods 2.1 Materials All the chemicals used in the present study were in the analytical reagent grade and solvents used were of HPLC grade. Reagents were used as such received without any further purification. Metal ions such as K+, Na+, Ca2+, Mg2+, Fe2+, Fe3+, Ag+, Zn2+, Mn2+, Cu2+, Co2+, Ni2+, Cd2+, Al3+, Cr3+, Pb2+ and Hg2+ were purchased from Merck and S.D. Fine chemicals. The anions of Cl-, Br-, I-, SCN-, CN-, H2PO4-, HSO4-, NO3-, AcO- and F- were purchased as their tetrabutylammonium salts from Sigma–Aldrich Pvt. Ltd. Absorption measurements were performed on JASCO V-630 spectrophotometer in 1 cm path length quartz cuvette with a volume of 2 mL at room temperature. Fluorescence measurements were made on a JASCO and F- 4500 Hitachi Spectrofluorimeter with excitation slit set at 5.0 nm band pass and emission at 5.0 nm band pass in 1 cm ×1 cm quartz cell. 1H and 13C NMR spectra were obtained on a Bruker 300 MHz NMR instrument with TMS as internal reference using DMSO-d6 as solvent. Standard Bruker software was used throughout. 19F NMR spectra were recorded at 293K on BRUKER 400 MHz FT-NMR spectrometers using DMSO-d6 as solvent. ElectroSpray Ionisation Mass Spectrometry (ESI-MS) analysis was performed in the positive/negative ion mode on a liquid chromatography-ion trap mass spectrometer (LCQ Fleet, Thermo Fisher Instruments Limited, US). Fluorescence microscopic imaging measurements were determined using Operetta High Content Imaging System (PerkinElmer, US) 2.2. Synthesis of (E)-4-bromo-2-(hydrazonomethyl) phenol, BHP An absolute alcoholic solution (50 ml) of 5-bromosalicylaldehyde (0.5gm, 2.49 mmol) was refluxed under hydrazine hydrate (in excess) for 5 hr and the pale yellow color solid product was collected after recrystallized with ethanol and ethyl acetate mixture (yield, 95 %). 1H NMR (300 MHz, DMSO-d6) δ (ppm): 8.92 (s, 1H), 11.89 (s, 1H), 7.53 (d, J = 8.7 Hz, 1H), 6.94 (d, J = 5.8 Hz, 1H); 13C NMR (75 MHz, DMSO-d6) δ (ppm): 161.36, 158.51, 135.84, 131.82, 120.86, 119.69, 106.72. 2.3 Photophysical analysis of BHP The optical mode analysis of BHP towards various cations/anions in DMSO/H2O (9:1, v/v) system was carried out by using absorbance and fluorescence spectroscopy. UV-visible and fluorescence analysis of BHP with cations were gauged by using their corresponding acetate salts of metal ions. Tetrabutylammonium salts of competing anions were used for the anionic sensing analysis. 2.4 Computational Studies The optimized geometrical and ground state energy level calculations of BHP were obtained by Density functional theoretical (DFT) calculations were executed using Gaussian 09 program 32 with the 6-311G basis set. The optimized geometries and the fluorescence enhancement of probe BHP complexed with Cd2+/F- ions were attained by DFT-B3LYP level theory using 6-311G and LANL2DZ basis sets. 2.5 Cytotoxicity studies HeLa cell lines were procured from the National Center for Cell Science (NCCS), Pune, India. Cell lines are kept in the Dulbecco's Modified Eagle's medium (DMEM) supplemented with 10% fetal bovine serum (FBS), 1% antimycotic and antibiotic solution was used in this study. The cells were kept in an incubator at 25 °C with humidified atmosphere comprising 5% of CO2 and 95% of air. HeLa cells were loaded over the wells of 96 well-culture plates with a density of 1 x 104 cells/well. After 48 h of incubation, previous DMEM medium was exchanged with new medium and BHP (dissolved in DMSO) was added in the range of 0-200 µM to all the wells and further incubated over 3h. Cytotoxicity of BHP was measured by using MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] assay. After incubation of HeLa cells with BHP, the medium was detached. Further, 100 μl of DMSO was added and the resulting formazan crystals were dissolved in DMSO. The cell viability was determined by measuring the absorbance of each well at 540-660 nm (formation of formazan) using a microplate reader. 2.6 In vivo fluorescence analysis in Zebrafish embryos The fluorescence imaging analysis was performed in four days old embryos. The embryos were seeded over F- ion alone for 2 h in the E3 medium. The E3 medium was prepared by dissolving 5.0 mM NaCl, 0.17mM KCl, 0.33mM CaCl2, 0.33mM MgSO4 ingredients in H2O (2L) and the pH 7.2 was adjusted by adding NaOH. The embryos were thoroughly washed with E3 medium. Successively, incubated embryos were sowed over 25 mM of BHP (in DMSO) solution for 3h. Further, embryos were washed again with E3 medium and fixed in 10% methyl cellulose solution for the good oriented images. The fluorescent images of BHP-F- were logged using high content screening microscopy. (Excitation wavelength of 482 nm and emission wavelength range of 500-700 nm). Results and discussion The probe, (E)-4-bromo-2-(hydrazonomethyl) phenol (BHP) has been synthesized by one step condensation between hydrazine and 5-bromosalicylaldehyde in ethanol (yield, 95 %) as shown in Scheme 1. The structure of the probe BHP was confirmed via 1H, 13C NMR analysis (Figure S1-S2, See ESI) 3.1. UV–vis spectral analysis of cations with BHP To investigate the cation sensing events of BHP towards different cations in DMSO/H2O (9:1, v/v) system by using UV-vis and fluorescence titration experiments. Initially, free probe BHP exhibited an absorption band at 367 nm and further addition of mono, di and trivalent cations such as Li+, K+, Ag+, Mn2+, Co2+, Ni2+, Cu2+, Zn2+, Fe2+, Hg2+, Na+, Mg2+, Ca2+, Pb2+, Fe3+ and Cr3+ exhibited tiny changes in absorption spectr due to their weak interaction towards BHP except Cd2+ ion as shown in Figure 1. Interestingly, upon titrated with Cd2+ ion, a new absorption band appeared at 470 nm due to the highly resonance induced charge transfer ability of bromo substituted salicyl moiety while the solution turns into dark yellow color from pale yellow. Increasing addition of Cd2+ ion results gradual reduction of both higher and lower energy bands at 367 nm and 470 nm respectively as depicted in Figure 2. Figure 1. UV-vis spectra of BHP (10 µM) with different cations (5 × 10-3 M) in DMSO/H2O (9: 1, v/v) system. Figure 2. UV-vis spectra of BHP (10 µM) with Cd2+ (0 – 100 µM) in DMSO/H2O (9: 1, v/v) system Besides, fluorescence response of probe BHP towards various cations such as Li+, K+, Ag+, Mn2+, Co2+, Ni2+, Cu2+, Zn2+, Fe2+, Hg2+, Na+, Mg2+, Ca2+, Pb2+, Fe3+ and Cr3+ including Cd2+ ion have been inspected in DMSO/H2O (9:1, v/v) system. Initially, the probe BHP displayed low intensed fluorescence band in free state. Addition of other commonly coexistent metal ions including Cd2+ ions exhibited trivial changes in fluorescence spectra. From these results, it is concluded that the probe BHP could serve as an excellent colorimetric assay for the detection of Cd2+ ions. 3.2. The sensing analysis of BHP towards anions Moreover, the anion binding attraction of BHP towards anions have been investigated in DMSO/H2O (9:1, v/v) system via both UV-visible and fluorescence spectral techniques. Initially the probe BHP showed the absorption band at 367 nm. Upon titrated with other anions such as Cl‒, Br‒, I‒, NO3‒, AcO‒, HSO4‒, H2PO4‒ and CN‒ were failed to alter the absorbance of the probe BHP except F‒ ions as shown in Figure 3a. Moreover, the incremental addition of F‒ ions (0-50 µM), the higher energy band at 367 nm was decreased along with the increment in new absorption band at 482 nm results an excellent ratiometric response. The new low energy band observed at 482 nm due to the deprotonation of–OH group present in salicyl moiety initiated by hydrogen bonding [Figure 3b]. At that affair, the solution turns into orange color from pale yellow and it was simply discerned by naked eye [Figure 4]. Besides, under identical condition, the fluorescence titration experiment of BHP was carried out in the presence of different anions. Interestingly, the probe BHP displayed low intensed fluorescence band at 601 nm and the other competing anions were failed to affect the fluorescence intensity except F‒ ions as shown in [Figure 5a]. Further, the incremental addition of F‒ ions triggers the enhancement in intensity results an excellent “turn on” fluorescence response due to the deprotonation and the inhibition of charge transfer state stimulated by resonance around the moiety [Figure 5b]. 3.3. Competitive experiments To gauge the selectivity and recognizing ability of BHP, competitive analysis was performed in the presence of varying concentration of F‒ ion (0-50 µM). Initially, the probe was treated with 5 × 10-3 M of different anions such as, CN-, I-, Br-, Cl-, NO2-, CH3COO-, H2PO4- and HSO4-. The other common competing anions were failed to bind with the probe BHP except F- ion [Figure 6 (a) and (b)]. From these observations, it is ensured that BHP could act as an excellent selective and sensitve chromogenic receptor for F- ions in real time monitoring and different biological applications. Figure 3 (a): UV-vis spectra of BHP with 5 × 10-3 M of other anions in DMSO/H2O (9: 1 v/v) system. (b) UV-visible spectra of BHP (5 µM) with F‒ (0-50 µM) in DMSO/H2O (9: 1 v/v) system. Figure 4. Naked eye detection of F‒ ions with BHP under visible light (top) and UV-lamp (bottom) and BHP with Cd2+ visible light only (bottom). Figure 5 (a): Fluorescence spectra of BHP (5µM) with 5 × 10-3 M of other anions in DMSO/H2O (9: 1, v/v) system. Excitation at 482 nm. Slit width is 5 nm. (b) Fluorescence spectra of BHP (5µM) with F‒ (0-50 µM) in DMSO/H2O (9: 1, v/v) system. Excitation at 482 nm. Slit width is 5 nm. Figure 6 (a): Selectivity analysis of F‒ ion with BHP in the presence of competing anions. Excitation at 480 nm, Slit width = 5 nm. (b) The blue bars represent the change of the fluorescence intensity of BHP with the consequent addition of other anions. The pink bars represent the addition of the competing anions to BHP. Excitation at 480 nm, Slit width = 5 nm. 3.4. Job’s plot analysis and calculation of binding constant of BHP for Cd2+/F‒ ions Furthermore, the Job’s plot [Figure 7(a) and (b)] analysis based on UV-visible and fluorescence titration experiments results confirmed the 1:1 binding stoichiometry of BHP with both Cd2+/F‒ ions respectively. To further support the binding stoichiometry of BHP with Cd2+/F‒ions, ESI-MS spectral analysis were performed. The ESI-MS spectral analysis of BHP-Cd2+/BHP-F‒ disclosed peaks at 327.45/258.28 corresponds to [BHP+Cd2++Na+]/[BHP+F‒+H++Na+] respectively (Figure S3-S4, See ESI). Furthermore, the 1:1 binding stoichiometry of BHP with F− ions was confirmed via 1H NMR titration profile (Figure 8) and 19F NMR. The deprotonation of ‒OH group present in the salicyl moiety was initiated by hydrogen bonding and the plausible binding mode of BHP with Cd2+ and F‒ ion is shown in Scheme 2. Further, the absorbance and fluorescence intensity changes of Cd2+ ions (A472 nm) and F‒ ions (A482 nm, I603 nm) were plotted against [Cd2+] and [F‒] respectively provided a good linear relationship between both BHP and Cd2+/F‒ ions (Figure S5, S6 and S7, See ESI). From absorbance and fluorescence titration profile, the binding constant values of BHP for Cd2+/F‒ ions were calculated using modified Benesi-Hildebrand method ions (Figure S8, S9 and S10, See ESI). The binding constant values of BHP with Cd2+ ions were found to be 4.26 ×10-4 M from UV-visible titration profile. Similarly, the binding constant values of BHP with F‒ ions were estimated to be 6.03 ×10-3 M / and 3.01 × 10-4 M from UV-visible and fluorescence titration profile respectively. The detection limits (LOD) of F‒ were calculated to be 0.05 nM respectively. Moreover, the LOD values of BHP signifies that the probe might be utilized for the quantitative determination of F‒ ions in environment and real system. Figure 7 (a) Job’s plot for BHP with F‒ ion. (b) Job’s plot for BHP with Cd2+ ion Scheme 2. Binding mode of BHP with Cd2+/F‒ ions 3.5. 1H NMR titrations of BHP with F- ions In addition, to confirm and highly supported the 1:1 binding stoichiometry of probe with F- ions, 1H NMR titrations was performed. Upon addition of F- ion (0.5 equiv), the proton signal corresponds to phenolic –OH group at 11.14 ppm was gradually decreased. Further, addition of 1 equiv. of F- ions to BHP showed the complete disappearance of –OH proton signal as depicted in Figure 8. Moreover, the binding stoichiometric ratio of F- ion with BHP was further supported by 19F NMR experiment. The (H2F)- signal appeared at -124.33 ppm (Figure S11-S12, See ESI) confirms the deprotonation process arose from phenolic –OH proton. Figure 8 1H NMR titration of BHP with F- (0-1equiv) in DMSO-d6 3.6. DFT calculations of BHP with Cd2+/F- ion To recognize the fluorescence enhancement of probe BHP after complexation with F-, DFT calculations were accomplished. The optimized structures of BHP, BHP-Cd2+ and BHP-F- were obtained using DFT/B3LYP-6-311G and B3LYP/LanL2DZ basis sets respectively. The frontier molecular orbital diagram obtained from optimized structure of BHP is presented in Figure 9. Upon binding with Cd2+ ion, the HOMO and LUMO are delocalized over the entire salicyl unit and their energy gap was reduced. It is noteworthy that inhibition of charge transfer in probe BHP renders the reduction of absorbance at 367 nm and 470 nm. Moreover, Complexation of F- ion to the probe BHP leads to lowering of HOMO-LUMO energy gap. In the presence of F-, HOMO and LUMO are distributed over the whole molecule of BHP. From these results, the F- ion was efficiently binded and complexed with BHP than Cd2+ ion. Figure 9. Frontier molecular orbital diagram of BHP, BHP-Cd2+and BHP-F‒ 3.7. Live cell Imaging analysis of BHP in HeLa cells / Zebrafish embryos The cell viability or cytotoxicity analysis of BHP (0–200 µM) against Human HeLa cells were performed using MTT assay. In 100 µM of BHP, cell viability was obtained as too high as 98%. (Figure S13, See ESI). Hence, the probe was sucessfully used for live cell imaging analysis of F- ions in Figure 10. Live cell fluorescence imaging analysis of BHP in HeLa cells. (a) Bright field images of HeLa cells incubated with BHP (25 µM) for 3h (b) Fluorescence merged images of HeLa cells incubated with BHP (25 µM) (c) Fluorescence image of HeLa cells incubated with BHP (25 µM) alone (d) Fluorescence image of HeLa cells incubated with BHP (25 µM) and 25 µM of F‒ ions for 1 h HeLa cells. Further, the HeLa cells were pre-treated with 25 µM of BHP alone for 3 h. Then HeLa cells were seaded with 25 µM of F- ions for 1h. In the absence of F- ions, the probe BHP exposed a weak yellow fluorescence. However, addition of F- ions to the probe BHP induced a bright orange fluorescence (Figure 10). These results endorsed that the probe BHP can be successfully utilized for the intracellular fluorescence imaging analysis of F- ions in HeLa cells. Besides, the exceptional cell viability output of BHP has been further explored in four days Zebrafish embryos. Zebrafish has positioned as a well-known vertebrate model in numerous biological applications. From this perspective, we have utilized also zebrafish embryos as a living animal model to expose the excellent imaging potential of BHP for the detection of F‒ ion in the biological environment (Figure 11) . Figure 11. Fluorescence imaging analysis of F‒ ion in 4 days old Zebrafish embryos developed with BHP and various concentrations of F‒ ion (a) bright field images of BHP (25 µM) alone, (b) fluorescence merged images of BHP and F- ion (25 µM) (c) fluorescence image of BHP (25 µM) alone (d) 25 µM of F‒ ion for 2 h continuously incubated with BHP (25 µM) for 3 h. 3.8. Evaluation of BHP with previous reports The probe BHP has valid and multi features such as single step synthesis, dual-mode recognition, turn-on fluorescence response and colorimetric change. The probe BHP displayed unique sensing property among other dual sensors. Table S1 compares the sensing performance of BHP with recently reported F‒ receptors. Amid, BHP exhibits too low limit of detection when compared with other previously reported chemoreceptors cited in table S1. Also, the limit of detection of BHP is within the range of recommended limits set by both EPA and WHO for F‒ Ions. Moreover, the fluorescence imaging experiments inferred that the probe BHP can be utilized as potential tool for mapping F‒ ion distribution in HeLa cells and Zebrafish embryos. Conclusions We have designed and synthesized a new chromogenic and fluorogenic probe based on salicylhydrazone derivative for the selective and sensitive detection of both Cd2+/F- ions by colorimetrically and fluorimetrically respectively. As per our knowledge, it is a novel simple hydrazone receptor for sensing carcinogenic heavy metal Cd2+ via colorimetric method and biologically significant F‒ ion by both colorimetric and fluorimetric methods. The binding constant value of Cd2+ ion was found to be 4.26×10-4 M by UV-visible method where as 6.03×10-3 and 3.01×10-4 M for F- ion by both UV-visible and fluorescence methods respectively. The limit of detection was found to be 0.05 nM for F- ion. The excellent biological potential of BHP has been successfully utilized for the detection of F- ions in Zebrafish embryos and human HeLa cells. Acknowledgments The authors acknowledge the financial support from the Council of Scientific and Industrial Research, Extramural Research, New Delhi, India (Grant No. 01(2901)17/EMR-II. The Department of Science and Technology, SERB, Extramural Major Research Project (Grant No. EMR/2015/000969), Department of Science and Technology, CERI, New Delhi, India (Grant No. DST/TM/CERI/C130(G) and we acknowledge the DST-FIST, DST-PURSE,DST-IRPHA, UPE programme and UGC-NRCBS, SBS, MKU for providing instrumentation facilities. References Jäkle, F. Chem. Rev. 2010, 110, 3985. 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Background: Blood donor programme is vital to any transfusion service. This process of blood donation involves voluntary and non remunerated blood donors. The objective of donor screening is to minimize the chances of transmitting infectious agents to recipients. Material and Method: The study was conducted during one year duration from 2017-2018. A total of 700 donors are deferred against 9556 blood donors recruited on the basis of clinical history and brief medical examination in blood bank and voluntary blood donation camps. Donors were deferred temporarily or permanently. Result: Out of 700 deferred donors; 546 were voluntary and 154 were replacement blood donors. Male (601) outnumbered female (99). There were 682 temporary and 18 permanent deferral. Most common age group for deferral was 17-30 yrs (490). Common causes included anemia(142), on medication(108), underweight(83) and high blood pressure(72). Conclusion: Donor selection process is a most important preliminary step in blood banking and should follow stringent medical and regulatory rules. Studying and analysing the profile of blood donors will help to identify sections of the population which could be targeted to increase the pool of voluntary blood donors. Every attempt should be made to decrease the donor deferral keeping in view the safety of both donor and recipients. Keywords: Blood donor, Deferral, Voluntary, Temporary, Permanent.

Background: Detection of Hepatitis B surface antibodies (anti-HBs) in the serum implies that an individual is immune to HBV infection either from vaccination or past infection, which usually persists for life. Aim: This study was aimed to determine the seropositivity of anti-HBs among HIV-seropositive patients and further appraise the effect of co-infection on their immunological status in Ojo, Lagos. Materials and Methods: A total of 74 blood samples collected from HIV positive patients were analysed for anti-HBs (HBsAb) by Immunochromatographic assay (Biotec, UK). CD4+ count was determined using BD FACS Count Automated analyser. Results: Out of the 74 HIV infected patients screened, 12 (16.2 %) males and 62 (83.8 %) females, 2 patients (2.7 %) were positive for anti-HBs, with no prevalence among the males and 2.7 % among the females. Anti-HBs was only recorded in HIV positive patients in the 21-30 years age group (8.7 %). The result showed anti-HBs lifelong protection to HBV among HIV patients with CD4+ Count ranging from 601-700 and 901-1000 to be 1.7 % and 1.7 % respectively. Protection of 3.5 % was reported among antiretroviral therapy (ART) naïve patients and 0 % protection among ART patients. Conclusions: Our findings showed prevalence of 2.7% HBsAb in the studied HIV positive patients with a protection of 3.5 % anti-HBs to HBV among antiretroviral therapy (ART) naïve patients in Ojo, Lagos. The low prevalence of HBsAb in HIV positive patients may be attributed to suppression of immune responses leading to the loss of previously developed protective antibody against HBV infection. We hereby suggest anti-HBs screening for all HIV positive patients and that those negative should be vaccinated to help them develop protective immunity to HBV infection.

Aims: This study aimed to investigate the interaction effect of nitrogen and phosphorus on curd yield and seed production of cauliflower. Study Design: The experiment was laid out at a randomized complete block design with three replications. Place and Duration of Study: Research farm of Bangabandhu Sheikh Mujibur Rahman Agricultural University, Gazipur, from September 2016 to March 2017. Methodology: The experiment consisted of two factors comprising three levels of nitrogen (270, 220,170 kg ha-1) and three levels of phosphorus (160,130,100 kg ha-1) with control of each nutrient. There were 10 treatment combinations resulting in 30 unit plots where each plot had two rows of 6 plants. Nitrogen and Phosphorus fertilizer were applied as per treatment in the form of Urea and TSP as a top dressing in two equal splits at the time of transplanting and 15 days after transplanting. Results: All the characters related to growth and yield of cauliflower showed significant variation by nitrogen and phosphorus levels at different days after transplanting. Among the different treatment combinations, N270P160 produced maximum plant height (50 cm), leaf area (700 cm2), plant weight (1003 g), curd diameter (18.3 cm), marketable curd weight (601 g) and marketable curd yield (18.5 t) at harvesting compared to control where nitrogen and phosphorus were not used. Moreover, the largest number of leaves (21.3) and curd weight (346 g) was observed by treatment combinations N220P160 at harvesting. For seed production, the highest number of siliqua plant -1 (1461), 1000 seed weight (4 g), seed yield plant-1 (41.1 g) seed yield ha-1 (1.5 t) and germination (97.7%)was also found from N270P160while the maximum number of seeds siliqua-1(20.5) was revealed by N220P160 compared to control treatment. Considering a different point of view, treatment combinations N270P160 can be marked more convenient for cauliflower production than others. Conclusion: It is concluded that the combinations of nitrogen (270 kg ha-1) and phosphorus (160 kg ha-1) would be appropriate for curd yield and seed production of cauliflower.

Amaç: Bruselloz, bulaşıcı bir enfeksiyon hastalığı olmakla birlikte diğer enfeksiyon türlerine benzer semptomlar göstermektedir. Bruselloz bir enfeksiyon hastalığı olması nedeniyle, C-reaktif protein (CRP) ve Ferritin gibi akut faz reaktanları değerlerini yükseltmektedir. Çalışmamızda Bruselloz tanısı konmuş hastalarda D vitamini, Ferritin, Folik Asit, Alanin aminotransferaz (ALT), Aspartat aminotransferaz (AST) ve Gama Glutamil Transferaz (GGT) düzeylerinin klinik açıdan öneminin belirlenmesi amaçlandı. Yöntemler: Çalışmaya, Dicle Üniversitesi Hastaneleri Enfeksiyon Hastalıkları kliniği ve polikliniğine başvurmuş, Bruselloz tanısı konmuş 50 hasta (vaka grubu) ile 25 sağlıklı kişi (kontrol grubu) yaşları 18 yaşından büyük ve cinsiyetleri benzer kişiler dahil edildi. Kontrol grubuna dahil edilme kriterleri, herhangi bir kronik hastalık olmaması, sigara alkol ve madde bağımlılığı olmamasıdır. Brucella Coombs Testi’nde titrenin 1/320 ve üzerinde bulunması Bruselloz açısından pozitif olarak değerlendirildi. Hasta ve kontrol gruplarında D vitamini, ferritin, folik asit, immüno kemilüminesans yöntemi ile Roche Cobas E 601 cihazı (Roche Diagnostics, ABD); CRP, ALT, AST ve GGT düzeyleri ise spektrofotometrik yöntem ile Beckman Coulter DxC 700 AU (Beckman Coulter, ABD) cihazıyla çalışıldı. Çalışma öncesinde Dicle Üniversitesi Tıp Fakültesi Girişimsel Olmayan Klinik Araştırmalar Etik Kurul İzni alındı. Bulgular: Bruselloz hastaların 26’sı (%52) kadın, 24’ü (%48) erkek olup; kontrol grubunun ise 12’si (%48) kadın, 13’ü(%52) erkekten oluşmaktadır. Aglütinasyon gözlenen örnekler pozitif, gözlenmeyenler ise negatif kabul edildi. D vitamini seviyesi kontrol grubunda, hasta grubuna göre anlamlı düzeyde düşük tespit edildi (p:0,007). Hasta grupta HGB, PLT, Folik asit değerleri kontrol grubuna göre daha düşük iken, ferritin düzeyi daha yüksek bulunmasına rağmen her iki grup arasında anlamlı fark bulunmadı. Sonuç: Bu çalışma sonucunda, hasta ve kontrol grubu karşılaştırıldığında, sadece vitamin D seviyesi hasta grubunda anlamlı olarak yüksek bulunmuştur. Hasta grubunda inflamasyon belirteçlerinden olan CRP ve ferritin yüksek olduğu tespit edildi. Bu sonuçlar bize, D vitamininin; bruselloz patogenezinde tanısal parametrelerde rol oynayabileceğini düşündürmektedir. Yapmış olduğumuz bu ve buna benzer çalışmalarla bölgesel epidemiyolojik verilerin elde edilmesine de katkı sağlayacağını düşünmekteyiz.

En el Ecuador, el gobierno desde el año 2010 asumió el control de admisión de los estudiantes a la Educación Superior, estableciendo nuevos métodos de ingreso a las universidades públicas del país, el mecanismo más significativo y de mayor debate e impacto fue la introducción del Examen Nacional para la Educación Superior (ENES) que se tomó por primera vez en 2011 y que modificó drásticamente el mecanismo de ingreso a la educación de tercer nivel en Ecuador y provocó multiples efectos que han sido poco estudidos; por lo que, con la presente investigación pretendemos contribuir a conocer este fenómeno mediante el analisis algunos de los problemas presentados a los estudiantes de la carrera de Agronomía de la Facultad de Recursos Naturales de la Escuela Superior Politécnica de Chimborazo (ESPOCH) de la ciudad de Riobamba, provincia Chimborazo. Para realizar el presente trabajo se aplicaron encuestas a los estudiantes que se encontraban matriculados en el primer semestre de la carrera de Agronomía para el período académico octubre de 2016 y marzo de 2017, considerando aspectos como la auto-identificación étnica, sexo, edad, provincia y cantón en la que se encuentra el colegio en el que se graduó como bachiller, el número de postulaciones que realizó para lograr un cupo en la carrera deseada por los estudiantes de acuerdo a los cupos ofertados en el Sistema Nacional de Nivelación y Admisión. También se consideran aspectos como el egreso económico que las familias tuvieron que realizar para preparar a sus hijos para obtener mejores puntajes y los gastos que están realizando para que ellos estudien en la ESPOCH en la ciudad de Riobamba. Entre los resultados mas significativos obtenidos tenemos que el el 50.4% mujeres y 49.6% hombres; el 83% de los estudiantes matriculados en el primer semestre de la carrera de Agronomía se autoidentificó como mestizo, el 15% como indígena y un 2% como afroecuatoriano; el 78% se graduó en colegios fiscales, el 13% en colegios particulares y el 9% en fiscomisionales. El 67.37% proviene de la provincia de Chimborazo y el 51.6% es de Riobamba. Para ingresar a la carrera el 55,8% realizó una postulación, 2 el 33,7%; 3 ocasiones el 7,4%; y, mas de 4 oportunidades el 2,1%. En cuanto al puntaje en la primera postulación los resultados fueron el 62,92% entre 701 y 800 puntos; el 26,97% entre 601 y 700 puntos; el 6,74% de 801 a 900 puntos. El 51% se inscribió en cursos prepratorios para realizar el examen; y el 73.91% dijo que le fue útil, pero solo el 6.74% alcanzo notas entre 801 y 900 puntos en la primera postulación puntajes que solo al 47% de los estudiantes les permitió lograr un cupo para la carrera que ellos deseaban. El 46% se vio en la necesidad de cambian su lugar de residencia para realizar sus estudios, de ellos el 64% lo hizo desde otra provincia, lo que implica que debe realizar gastos para su estadía. Este es un trabajo pionero, que parte desde el punto de vista de los estudiantes y presenta las primeras evidencias empíricas y nos da pistas para tratar de comprender lo que está sucediendo con la aplicación del ENES, tanto en los efectos como en las consecuencias que está teniendo la implementación del sistema de ingreso a las universidades ecuatorianas que tiene a esta prueba como su eje angular, también proporciona datos para dilucidar algunos mitos que se han construido alrededor de este instrumento, que a su vez pueden servir para la realización de nuevas investigaciones. In Ecuador, the government since 2010 assumed control of admission of students to Higher Education, establishing new methods of admission to public universities in the country, the most significant mechanism and the most debate and impact was the introduction of the Exam National for Higher Education (ENES) which was taken for the first time in 2011 and drastically modified the mechanism of entry to third level education in Ecuador and caused multiple effects that have been little studied; so, with this research we intend to contribute to know this phenomenon by analyzing some of the problems presented to the students of the career of Agronomy of the Faculty of Natural Resources of the Polytechnic School of Chimborazo (ESPOCH) of the city of Riobamba, Chimborazo province.To carry out this work, surveys were applied to students who were enrolled in the first semester of the Agronomy career for the academic period October 2016 and March 2017, considering aspects such as ethnic self-identification, sex, age, province and the canton in which the college where he graduated as a bachelor is located, the number of applications he made to achieve a quota in the career desired by the students according to the quotas offered in the National Leveling and Admission System. They also consider aspects such as the economic income that families had to make to prepare their children to obtain better scores and the expenses, they are making so that they study at the ESPOCH in the city of Riobamba. Among the most significant results obtained we have that 50.4% women and 49.6% men; 83% of students enrolled in the first semester of Agronomy self-identified as mestizo, 15% as indigenous and 2% as Afro-Ecuadorian; 78% graduated in tax colleges, 13% in private schools and 9% in fiscomisionales. 67.37% comes from the province of Chimborazo and 51.6% comes from Riobamba. To enter the race, 55.8% made a nomination, 2 33.7%; 3 occasions 7.4%; and, more than 4 opportunities 2.1%. As for the score in the first application, the results were 62.92% between 701 and 800 points; 26.97% between 601 and 700 points; 6.74% from 801 to 900 points. 51% enrolled in prep courses to take the exam; and 73.91% said that it was useful, but only 6.74% reached scores between 801 and 900 points in the first postulation scores that only 47% of the students allowed them to achieve a quota for the career they wanted. 46% saw the need to change their place of residence to carry out their studies, of which 64% did so from another province, which implies that they must make expenses for their stay. This is a pioneering work, which starts from the point of view of the students and presents the first empirical evidence and gives us clues to try to understand what is happening with the application of the ENES, both in the effects and in the consequences that are having the implementation of the entrance system to the Ecuadorian universities that has this test as its cornerstone, it also provides data to elucidate some myths that have been built around this instrument, which in turn can be used to carry out new research. Palabras Claves: Examen Nacional de Educación Superior, educación superior, gratuidad, asignación de cupos, postulaciones. Keywords: National Examination of Higher Education, higher education, gratuity, quota allocation, applications.

The present study examined the performance of the 100 kWp agri-voltaic systems at ICAR-CAZRI, Jodhpur. The average PV generation from the 100 kWp AVS connected to the grid via a bi-directional energy meter or net meter was about 342 kWh day-1. The average yields of mung bean, moth bean, and cluster bean in the inter-row spaces between the modules in the two-row and three-row PV array were 1155, 670, and 2008 kg.ha-1, respectively. Thus, there were 4.6%, 8.6%, and 11.8% reductions in the yield of mung bean, moth bean, and cluster bean, respectively, in inter-row spaces between the panels compared to control. During Rabi (irrigated) 2021-22, the yield of chickpea, cumin, and isabgol (2490, 1000, and 700 kg ha-1, respectively) in interspaces of the AVS was lower than that of the control (2670, 1120, and 760 kg ha-1, respectively). AVS resulted in a yield reduction of 6.6, 10.3, and 7.8% in chickpea, cumin, and isabgol, respectively, compared to the control. The AVS shows the maximum IRR (20.38%), while PV-GM shows the lowest (19.42%) at the prevailing bank loan interest rate of 12%. The PBP estimated 7.47 years for AVS with irrigated crops and 8.11 years for AVS with rainfed crops, while it was 8.61 years for PV-GM. The lower value of the discounted PBP, the faster the repayment of the investment cost. Therefore, the highest LCOE (INR 3.45 kWh-1) is estimated based on the break-even electricity tariff in PV-GM, and the lowest LCOE is calculated in AVS (INR 3.17 kWh-1).

AbstractThe diffusion of oxygen, lithium, chlorine, and fluorine in CVD diamond films was performed under bias at 700 and 1000 °C. SIMS and Auger analyses were used to determine the impurity concentration. After diffusion, the concentrations of Li and O in the diamond films were found to be of the order of (3–4)×1019 cm-3. The fluorine concentration was of order of (l-2)×1017 cm-3. The conductivity was p-type. The change in the resistivity due to diffusion was nearly nine orders of magnitude for the sample diffused under electric field, and six orders of magnitude for the samples diffused without field. No dependence of the impurity concentration on the applied bias was observed except for fluorine. The fluorine concentration dependence on the electric field indicates that fluorine may have formed a shallow level in the diamond band gap. The fact that large concentrations of impurities can be diffused into diamond films at relatively low temperatures indicates the presence in the films of many lattice defects (including grain boundaries).