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Koothan, Thillai. "Structural and functional analysis of zona pellucida". Thesis, University of Edinburgh, 1994. http://hdl.handle.net/1842/19905.
Pełny tekst źródłaChapman, Jamie. "The marsupial zona pellucida : its structure and glycoconjugate content". Title page, abstract and contents only, 2003. http://web4.library.adelaide.edu.au/theses/09PH/09phc4661.pdf.
Pełny tekst źródłaGaboriau, David Claude Andre. "Binding of boar sperm proacrosin to the zona pellucida". Thesis, University of Cambridge, 2007. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.613079.
Pełny tekst źródłaChung, Man-kin, i 鍾文健. "Biological characterization of cumulus glycodelin on humanspermatozoa-zona pellucida interaction". Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2009. http://hub.hku.hk/bib/B42182633.
Pełny tekst źródłaLacey, Helen Ann. "Production and characterisation of recombinant human zona pellucida glycoprotein 2". Thesis, University of Sheffield, 1998. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.322910.
Pełny tekst źródłaWitzendorff, Dorothee von. "Das Glykom und Proteom der porcinen Zona pellucida ein massenspektrometrischer Ansatz /". [S.l.] : [s.n.], 2006. http://deposit.ddb.de/cgi-bin/dokserv?idn=981929931.
Pełny tekst źródłaPfisterer, Susanne Charlotte Martha. "Herstellung, Charakterisierung und Einsatz von anti-Zona pellucida Protein 3 Peptidantiseren". [S.l.] : [s.n.], 1999. http://deposit.ddb.de/cgi-bin/dokserv?idn=958759006.
Pełny tekst źródłaChung, Man-kin. "Biological characterization of cumulus glycodelin on human spermatozoa-zona pellucida interaction". Click to view the E-thesis via HKUTO, 2009. http://sunzi.lib.hku.hk/hkuto/record/B42182633.
Pełny tekst źródłaRingleb, Jennifer. "Identifikation antigener Determinanten des ZPB2-Proteins der Hauskatze und Charakterisierung ihrer kontrazeptiven und immunogenen Eigenschaften". Phd thesis, [S.l. : s.n.], 2004. http://deposit.ddb.de/cgi-bin/dokserv?idn=974115282.
Pełny tekst źródłaTörmälä, R. M. (Reeta-Maria). "Human zona pellucida abnormalities:a genetic approach to the understanding of fertilization failure". Doctoral thesis, Oulun yliopisto, 2016. http://urn.fi/urn:isbn:9789526212982.
Pełny tekst źródłaTiivistelmä Diagnostiikan kehityksestä huolimatta hedelmättömyyden syy jää edelleen epäselväksi 10–20 %:ssa tapauksista. Niissä hedelmättömyyden taustalla voivat olla munasolun hedelmöittymiseen ja kohtuun kiinnittymiseen liittyvät ongelmat, jotka voivat osittain johtua geneettisistä syistä. Alkiokuori on munasolua ja varhaista alkiota ympäröivä rakenne, joka osallistuu munarakkulan kehittymiseen, munasolun hedelmöittymiseen ja alkion tarttumiseen kohdun limakalvolle. Ihmisellä alkiokuori muodostuu neljästä tunnetusta alkiokuoriproteiinista (ZP1–ZP4). Tutkimuksessa selvitettiin alkiokuoriproteiineja koodittavien geenien vaikutusta hedelmällisyyteen potilailla, joilla koeputkihedelmöitys ei ollut tuottanut yhtään hedelmöittynyttä munasolua (engl. total fertilization failure, TFF) tai joiden munasoluissa havaittiin alkiokuoren rakennemuutoksia (engl. zona anomalies, ZA). Lisäksi selvitettiin alkiokuoriproteiinien ja niiden lähetti-RNA:n esiintymistä sikiöiden ja aikuisten munasarjoissa. TFF- ja ZA-potilaiden alkiokuoriproteiineja koodittavista geeneistä löytyi yhteensä 34 nukleotidimuutosta. Muutoksista kolme oli uusia löydöksiä, mutta suurin osa oli ennalta tunnettuja yhden nukleotidin polymorfioita eli geneettisiä monimuotoisuuskohtia. TFF-potilailla havaittiin ZP1- ja ZP3-geeneissä keskimäärin enemmän polymorfioita kuin verrokeilla. Myös osa yleisimmistä alkiokuoren rakennemuutoksista voidaan mahdollisesti selittää ZP1–ZP4-geeneistä löytyneillä polymorfioilla. Sikiöllä ZP3:n ilmentyminen oli havaittavissa jo 11. raskausviikolla, mutta voimakkainta se oli primordiaalivaiheen munarakkuloiden muodostumisen aikaan 20. raskausviikolla. Tämä voi viitata siihen, että ZP3 saattaa osallistua primordiaalivaiheen munarakkulan kehittymiseen ennen varsinaisen alkiokuoren muodostumista. ZP-geenien säätelytekijän FIGLA:n esiintyminen lisääntyi 20. raskausviikolla, mikä tukee aikaisempia havaintoja FIGLA:n merkityksestä munarakkulan kehittymisen aktivaatiossa ja primordiaalivaiheen munarakkuloiden muodostumisessa. Tämä tutkimus tuo lisätietoa alkiokuoren merkityksestä munasolun hedelmöittymisessä ja syventää tietämystämme alkiokuoren muodostumisesta ihmisellä. Hedelmättömyyden taustalla olevien geneettisten tekijöiden tunteminen voi parantaa lapsettomuuspotilaiden hedelmällisyyden arviointia ja auttaa löytämään heille parhaiten sopivan hoidon
Hägele, Wolfgang. "Herstellung, Charakterisierung und Einsatz von Anti-Zona pellucida-Protein 2-Peptid-Antiseren". [S.l.] : [s.n.], 1998. http://bibd.uni-giessen.de/gdoc/1998/uni/D980008.PS.
Pełny tekst źródłaBalbach, Melanie [Verfasser]. "Action of zona pellucida glycoproteins in mouse and human sperm / Melanie Balbach". Bonn : Universitäts- und Landesbibliothek Bonn, 2017. http://d-nb.info/1166754790/34.
Pełny tekst źródłaBastiaan, Hadley Saville. "The effect of solubilized homologous zona pellucida on the human acrosome reaction, sperm-zona binding and motion characteristics of capacitated human spermatozoa". Thesis, Stellenbosch : Stellenbosch University, 2003. http://hdl.handle.net/10019.1/53630.
Pełny tekst źródłaENGLISH ABSTRACT: CHAPTER 1 provides literature based background information to emphasize the use of a sequential, multistep diagnostic schedule for couples in an assisted reproductive program as well as the clinical importance of sperm morphology as recorded by strict criteria during the diagnostic approach of the infertile couple. Furthermore, the chapter includes evidence underlining the growing need for the implementation of the physiologically induced acrosome reaction as an important contribution to the assisted reproductive program. The zona pellucida-induced acrosome reaction (ZIAR), sperm-zona interaction as well as computer-assisted semen analyses were investigated. CHAPTER 2 provides detailed experimental protocols of the materials and methods used in the study. CHAPTERS 3-6 each represent a separate study that was prepared as a scientific paper and encompass the experimental research undertaken in the reproductive biology research laboratory at Tygerberg Hospital to address important aspects of human acrosome processes. In the first study, we aimed to evaluate the regulatory role of the Gi-like protein during the AR of normal sperm donors and the role of intact acrosomes during sperm-zona binding. It seems that pertussis toxin-sensitive Gi-like protein in human spermatozoa plays an important regulatory role in the ZIAR and this underlines the importance of intact acrosomes during sperm-zona binding. In the second study, we aimed to evaluate the relationship between the ZIAR and the percentage normal spermatozoa as well as the sperm-ZP binding potential among men referred for a routine semen analysis. ZIAR testing should become part of the second level of male fertility investigations, i.e., sperm functional testing, since 15% of the andrology referrals revealed an impaired AR response to solubilized ZP. In the third study, we aimed to evaluate the possible relationships between the sperm morphology, the acrosome responsiveness to solubulized human ZP and the sperm-zona binding potential among consecutive andrology referrals and randomly selected IVF cases. ZIAR results provide further information regarding dysfunctional sperm and can be used as an additional diagnostic test since the results predicted fertilization failure during IVF treatment. In the fourth study, we aimed to evaluate changes in the sperm motion characteristics and the occurrence of hyperactivated motility after exposure to ZP among andrology referrals. Solubilized human ZP induces hyperactivated motility among sperm populations that have been capacitated under laboratory conditions. Capacitated spermatozoa have an elevated percentage hyperactivated cells that correlate with the percentage normal spermatozoa in the ejaculate. CHAPTER 7, the general discussion, is brief and concise to avoid unnecessary repetition, underlines the validity of a sequential, multistep diagnostic approach and concludes with the recommendation that the ZIAR should form part of the diagnostic tools in the assisted reproductive program.
AFRIKAANSE OPSOMMING: HOOFSTUK 1 bestaan uit ’n omvattende agtergrondstudie wat bestaan uit die ontwikkeling van diagnostiese toetse, die hantering van die egpaar in die reproduktiewe ondersteunings-program asook op die kliniese belang van spermmorfologie. Die toenemende behoefte aan die implementering van die fisiologies-geinduseerde akrosoomreaksie, as ’n belangrike bydrae tot die reproduktiewe ondersteuningsprogram, word ook beklemtoon. Die zona pellucida geinduseerde akrosoomreaksie (ZIAR), sperm-zonabinding asook rekenaar-bemiddelde semenanalises is ondersoek. HOOFSTUK 2 dek gedetailleerde eksperimentele protokolle van die materiale en metodes wat in die studie gebruik is. HOOFSTUKKE 3-6 behandel die eksperimentele navorsing wat in die laboratorium van die reproduktiewe biologie-eenheid te Tygerberg hospitaal uitgevoer is en wat as ses afsonderlike wetenskaplike publikasies aangebied word. Die doel van die eerste studie was om die regulerende rol van Gi-proteiene tydens die AR van normale spermdonors asook die rol van intakte akrosome tydens sperm-zonabinding te evalueer. Dit kom voor asof Gj-protei'ene in spermatozoa ’n belangrike regulerende rol in die ZIAR speel. Dit beklemtoon die belangrikheid van intakte akrosome tydens sperm-zonabinding. Die doel van die tweede studie was om die verhouding tussen die ZIAR en die persentasie normale spermatozoa asook die sperm-zonabindingspotensiaal tussen mans wat vir ’n roetine semenanalise verwys is te evalueer. ZIAR-toetsing moet deel uitmaak van die tweede vlak van manlike fertiliteitsondersoeke, d.w.s. funksionele toetsing, aangesien 15% van die andrologie pasiente ’n verswakte AR respons tot opgeloste ZP openbaar. In die derde studie was die doel om die moontlike verhoudinge tussen sperm-morfologie, die ZIAR en die sperm-zonabindingspotensiaal onder opeenvolgende andrologie-pasiente asook lukraak geselekteerde IVB-pasiente te evalueer. Die ZIAR-resultate bied verdere informasie aangaande disfunksionele spermatozoa en kan gebruik word as ’n addisionele diagnostiese toets aangesien hierdie resultate mislukte bevrugting tydens IVB behandeling voorspel. Die vierde studie het ten doel gehad om veranderinge in spermmotiliteitseienskappe asook hiperaktiwiteit na die blootstelling aan opgeloste zona onder andrologiepasiente te evalueer. Daar is afgelei dat opgeloste menslike zona hiperaktiwiteit induseer in spermpopulasies wat onder gunstige laboratoriumkondisies gekapasiteer is. Die gekapasiteerde spermatozoa het ’n verhoogde persentasie gehiperaktiveerde spermatozoa getoon wat met die persentasie normale spermatozoa in die ejakulaat korreleer. In HOOFSTUK 7 word aangetoon dat dit noodsaaklik is om die diagnostiese skedule by die hantering van die onvrugbare egpaar te gebruik asook dat die ontwikkeling van die funksionele toestand belangrik is vir die bepaling van ZIAR.
Lowe, Jeanette. "Phage Display to Identify Peptides Binding to or Penetrating the Mouse Zona Pellucida". Thesis, Virginia Tech, 1999. http://hdl.handle.net/10919/35902.
Pełny tekst źródłaMaster of Science
Lunn, Matthew O'Brien. "A Morphological Study of the Canine Zona Pellucida: A Heterogeneous Ultrastructure and Barrier". University of Dayton / OhioLINK, 2011. http://rave.ohiolink.edu/etdc/view?acc_num=dayton1311785290.
Pełny tekst źródłaau, joyceand@iinet net, i Jocye A. Eade. "Investigating porcine and feline zona pellucida as immunocontraceptive antigens in the female domestic cat". Murdoch University, 2007. http://wwwlib.murdoch.edu.au/adt/browse/view/adt-MU20070717.124229.
Pełny tekst źródłaEade, Joyce A. "Investigating porcine and feline zona pellucida as immunocontraceptive antigens in the female domestic cat /". Access via Murdoch University Digital Theses Project, 2007. http://wwwlib.murdoch.edu.au/adt/browse/view/adt-MU20070717.124229.
Pełny tekst źródłaWong, Siu-tak, i 黃兆德. "Priming effect of glycodelin-A on zona pellucida induced acrosome reaction in human spermatozoa". Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2008. http://hub.hku.hk/bib/B41508439.
Pełny tekst źródłaWong, Siu-tak. "Priming effect of glycodelin-A on zona pellucida induced acrosome reaction in human spermatozoa". Click to view the E-thesis via HKUTO, 2008. http://sunzi.lib.hku.hk/hkuto/record/B41508439.
Pełny tekst źródłaEade, Jocye A. "Investigating porcine and feline zona pellucida as immunocontraceptive antigens in the female domestic cat". Thesis, Eade, Jocye A. (2007) Investigating porcine and feline zona pellucida as immunocontraceptive antigens in the female domestic cat. PhD thesis, Murdoch University, 2007. https://researchrepository.murdoch.edu.au/id/eprint/59/.
Pełny tekst źródłaEade, Jocye A. "Investigating porcine and feline zona pellucida as immunocontraceptive antigens in the female domestic cat". Eade, Jocye A. (2007) Investigating porcine and feline zona pellucida as immunocontraceptive antigens in the female domestic cat. PhD thesis, Murdoch University, 2007. http://researchrepository.murdoch.edu.au/59/.
Pełny tekst źródłaBates, Melodie Joy. "Endocrine correlates of free-ranging African elephant (Loxodonta africana) treated with porcine zona pellucida vaccine". Diss., University of Pretoria, 2010. http://hdl.handle.net/2263/25181.
Pełny tekst źródłaDissertation (MSc)--University of Pretoria, 2010.
Production Animal Studies
unrestricted
Oehninger, Sergio C. "The interaction between human spermatozoa and its homologous zona pellucida : scientific advances and clinical significance". Thesis, Stellenbosch : Stellenbosch University, 2002. http://hdl.handle.net/10019.1/53132.
Pełny tekst źródłaENGLISH ABSTRACT: Infertility is a very common problem worldwide. Recent data have shown that disorders of the male represent the most common single defined cause of infertility. This proposal examines the clinical significance and fundamental physiological aspects of human gamete interaction. These studies are focused on the assessment of the cellular-molecular mechanisms involved in human sperm binding to its homologous zona pellucida resulting in the physiologic induction of the acrosome reaction. We have developed and validated in vitro bioassays that assess specific steps of the fertilization process that are critical for early embryo development. The results of our translational research have already had a significant impact on the overall evaluation of male infertility and on the clinical management of the infertile man in the assisted reproduction arena. Furthermore, the unveiling of the basic mechanisms involved in human gamete interaction will ultimately allow for both (i) the development of new male reproductive diagnostic capabilities and (ii) the design of improved and safer therapies aiding conception in childless couples suffering from male infertility.
AFRIKAANSE OPSOMMING: Menslike onvrugbaarheid is 'n algemene wêreldwye probleem en onlangse data toon aan dat die manlike factor die grootste enkel bydraende factor tot hierdie toestand is. Die werk loods 'n intensiewe ondersoek na die kliniese betekenis en basiese fisiologiese aspekte wat 'n rol tydens spermsel en eisel interaksie speel. Hoofstuk 3 fokus op die sellulêre en molekulêre meganismes wat betrokke is tydens spermsel en eisel binding wat gevolglik lei tot akrosoomreaksie van die spermsel. Die werk verteenwoordig die resultate van 10 jaar se navorsing tussen die kandidaat en die promoter. Dit gee oorsprong aan 'n reeks bio-toetse wat die bevrugtingsproses koriografiese ontleed en verskaf dus 'n stap-vir-stap uiteenseting van menslike bevrugting en gevolglike embrio ontwikkeling. Die resultate in Hoostuk 4 bring vernuwing in die begrippe van die manlike faktor en die rol in die kinderlose huwelik. Die resulate soos in Hoofstuk 3 en 4 uiteengesit, vorm nie net die basis vir die moontlike ontwikkeling van nuwe diagnostiese benaderings tot die hantering van die man nies maar speel oojk 'n rol die daarstelling van verbeterde terapeutiese hantering van die kinderlose egpaar. Hoofstuk 5 gee kortliks riglyne en aanbevelings tot opsigte van die gebruik van die spermsel-zona pellucida bindingstoets en akrosomreaksie. Die kandidaat bevel aan dat die genoemde twee bio-toetse deel van die laboratorium ondersoeke van die man gebruik moet word.
Kuske, Michael [Verfasser]. "Das Zusammenspiel der Proteasen Akrosin und Ovastacin bei der Härtung der Zona pellucida / Michael Kuske". Mainz : Universitätsbibliothek der Johannes Gutenberg-Universität Mainz, 2021. http://d-nb.info/1231433469/34.
Pełny tekst źródłaKaskar, Khalied. "Evaluation of quantitative motility and zona pellucida binding of human spermatozoa in an assisted reproductive programme". University of the Western Cape, 1994. http://hdl.handle.net/11394/8416.
Pełny tekst źródłaMale factor disorders affect more than 30% of infertile couples. Thus, it has become important to perform a andrological consultation and a basic semen evaluation in all male partners of couples consulting for infertility. The advent and development of assisted reproductive technologies has not only improved clinical results but also enhanced our basic understanding of the physiology of sperm and sperm preparation methods. Assisted reproduction has become among the more successful therapeutic modalities for a wide variety of sperm function disorders e.g. artificial insemination and in vitro fertilization (IVF) (Acosta et al. 1989). It is clear from recent experience that patients with male infertility showing oligozoospermia, asthenozoospermia, teratozoospermia (sometimes in combination), male immunological factor (antisperm antibodies) or ejaculatory problems as well as congenital abnormalities, can be successfully treated with IVF and embryo transfer. Prerequisite pre-fertilization changes by sperm, termed "capacitation" (Austin 1952) provides sperm with the capacity to fertilize eggs. These processes are generally regarded as encompassing all pre-fertilization changes occurring in sperm up 'to, but not including, loss of the acrosome (Bedford 1970). The endpoints of capacitation are often described as the acrosomal loss as well as changes in the motion characteristics. Capacitation alters the pattern of motility exhibited by freely swimming sperm, changing from a fairly rigid flagellar beat pattern to one of extreme flexure, often associated with increased thrust (Johnson et al. 1981) , which is referred to as hyperactivated motility (Yanagimachi 1981). without the transition to hyperactivated motility, sperm are unable to penetrate the zona pellucida (Fraser 1981), and possibly unable to fertilize eggs. Hyperactivation per se is marked by increased curvature in swimming trajectories and/or increased lateral displacement of the sperm head along their path (Burkman 1984). However, the physiological role of this change in motility is not clearly understood because almost all relevant data have been obtained under in vitro conditions. The association of specific seminal characteristics (sperm concentration, percentage motile cells and percentage normal sperm morphology) with the success rate of assisted particular has been The analysis of 1984; the reproductive techniques and IVF in under great scrutiny (Mahadevan and Trounson relationships between conventional semen parameters and fertilization rates in vitro has shown that sperm motility, concentration and morphology must be considered in estimating opportunities for successful intervention, as in the case of IVF and gamete intrafallopian transfer (GIFT) (Oehninger and Hodgen 1991). A reduction in the percentage of progressive motility alone does not seem to have a significant impact on IVF results unless it is below a threshold value of 10%. The semen sample should have an acceptable sperm concentration and morphology and/or that at least 1.5 X 106 motile spermatozoa can be recovered after swim-up separation (Acosta et al. 1989).
Levy, Maria Patricia Morales. "Development of a DNA vaccine for population control : a zona pellucida based nucleic acid vaccine (ZP-NAV) /". For electronic version search Digital dissertations database. Restricted to UC campuses. Access is free to UC campus dissertations, 2005. http://uclibs.org/PID/11984.
Pełny tekst źródłaShahror, Rami Ahmad Nawaf. "Characterization of the Transcription Factor NF‐Y in the Regulation of Zona Pellucida Genes in Zebrafish Ovary". Thesis, Örebro universitet, Institutionen för naturvetenskap och teknik, 2011. http://urn.kb.se/resolve?urn=urn:nbn:se:oru:diva-30318.
Pełny tekst źródłaPetersen, Claudia Guilhermino. "Correlação da maturação e da morfologia do oócito humano com as características do fuso meiótico e da zona pelúcida /". Botucatu : [s.n.], 2009. http://hdl.handle.net/11449/106388.
Pełny tekst źródłaBanca: Anice Maria Vieira Camargo Martins
Banca: Edson Borges
Banca: Jorge Nahas Filho
Banca: Ana Lúcia Kalinin
Resumo: Nas últimas décadas, alguns parâmetros laboratoriais têm sido propostos para identificar o potencial de saúde oocitária em Reprodução Assistida: 1. Maturidade nuclear e citoplasmática (pesquisa); 2. Dismorfismo (anomalias intracitoplasmáticas); 3. Fertilizaçao. Habitualmente, esses parâmetros (exceto a maturação citoplasmática) são avaliados pelo simples emprego de sistemas de microscopia de luz convencional. Nos últimos oito anos, foram relatados diversos estudos em oócitos humanos sobre a birrefringência da zona pelúcida (BF-ZP), a espessura da ZP, e a visualização do fuso meiótico (FM). Entretanto, ainda é discutível o poder desses novos parâmetros na seleção de óvulos com o pontecial de originar embriões com taxas de implantação elevadas, além disso, seu sistema de visualização e quantificação é complexo e dispendioso. O objetivo deste estudo foi investigar uma possível correlação entre esses parâmetros tradicionais com a BF-ZP, espessura da ZP e o FM. Dessa forma, um total de 73 pacientes que participaram do programa de injeção intracitoplasmática de espermatozóide (ICSI) no Centro de Reprodução Humana "Prof Franco Júnior" tiveram seus óvulos avaliados quanto a maturação nuclear (n=83), maturação citoplasmática (n=29), dismorfismo (n=280) e fertilização (n=216) sendo os resultados correlacionados com a imagem do FM (presença ou ausência), a intensidade da BF-ZP (positiva ou negativa) e a espessura da ZP. Os dados finais revelaram uma fraca correlação entre a maturação, o dismorfismo e a fertilização oocitária com a imagem do FM, a intensidade da BF-ZP, e a espessura da ZP. Entretanto, esses parâmetros poderiam ser responsáveis por identificar pontos biológicos diferentes do potencial de qualidade oocitária. Assim sendo, seria uma medida coerente, a utilização desses parâmetros, em forma conjunta, através de um escore de qualidade oocitária.
Abstract: During the last decades, three laboratorial parameters have been proposed to identify the potential of the oocyte quality in ART: 1- Nuclear and cytoplasmic maturation; 2-Dysmorphims (intracytoplasmic abnormalities); 3 - Fertilization. These parameters (except the cytoplasmic maturation) are assessed by a simple system of conventional light microscopy. In the last eight years, numerous studies about zona pellucida birefringence (BF-ZP), zona pellucida thickness and meiotic spindle visualization have been reported. However, it is still controversial the power of these parameters in selecting oocyte with potential to develop into implantation-competent embryos. Furthermore, the microscopy system is complex and expensive. The present study aims to evaluate whether there is any correlation between the classical parameters with BF-ZP, ZP thickness and spindle image. A total of 73 patients who participated in an ICSI program had their oocytes evaluated such nuclear maturation (n=83), cytoplasmic maturation (n=29), dysmorphism (n=280) and fertilization (n=216) being its results correlated to the spindle image (presence or absence), BF-ZP intensity (positive or negative) and ZP thickness. The final results showed a low correlation between maturation, dysmorphism and fertilization compared to spindle image and ZP characteristics (BF-ZP, ZP thickness). However, these factors can be responsible to identify specific biological differ from the oocyte potential. Concluding, the use of these factors in association, as a score (oocyte quality score), could be the correct evaluation.
Doutor
Gan, Yong Chen Zhen. "Model-based simulations of the piercing process in piezo-ICSI". Diss., Columbia, Mo. : University of Missouri--Columbia, 2008. http://hdl.handle.net/10355/7111.
Pełny tekst źródłaParkinson, Christine Anne. "Cloning and characterisation of a zebrafish cDNA encoding a novel member of the Zona pellucida family of proteins". Thesis, King's College London (University of London), 2002. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.271294.
Pełny tekst źródłaDu, Plessis S. S. (Simon Stephanus). "A study of the intracellular signalling events involved in the zona pellucida-induced acrosome reaction in human spermatozoa". Thesis, Stellenbosch : Stellenbosch University, 2002. http://hdl.handle.net/10019.1/52709.
Pełny tekst źródłaENGLISH ABSTRACT: In this study the author presents new data that will shed light on the fairly nebulous knowledge of intracellular pathways involved in the physiologically induced acrosome reaction and the subsequent events leading to fertilization. The zona pellucidainduced acrosome reaction, sperm-zona interaction as well as various sperm motion characteristics were investigated. The first part of the study focussed on the role of extracellular signal regulated kinase (ERK), a member of the family of mitogen activated protein kinases, during the zona pellucida-induced acrosome reaction and sperm-oocyte interaction. It was shown that the inhibition of ERK significantly reduced the zona pellucida-induced acrosome reaction as measured by fluorescent microscopy. This suggests that ERKs are directly or indirectly involved in the signal transduction pathway through which the human sperm acrosome reaction is induced by the zona pellucida. In an attempt to provide further proof that ERK was involved in human acrosome signalling hemizona assays were employed to test sperm-oocyte binding. From these sperm-oocytebinding experiments it was clear that the inhibition of ERK leads to increased binding. These results support the idea that the zona pellucida-induced acrosome reaction, and therefore the physiologically relevant exocytotic event, is regulated by an ERKmediated signal transduction process. In the second part of the study the significance of phosphatidylinositol 3-kinase (PI3K) in the process of human sperm motility, acrosome reaction and sperm-oocyte binding, was investigated by employing the specific PI3K, LY294002. PI3K inhibition increased the percentage motility and percentage progressive motility in asthenozoospermia patients. Under the present laboratory conditions PI3K inhibition furthermore did not influence the acrosome reaction, whilst it enhanced sperm-oocyte binding. These results therefore imply that PI3K negatively affect sperm motility and zona-binding. In the last part of the study the possible effects of intracellular cGMP accumulation via acute in vivo sildenafil citrate (ViagraTM) administration was investigated on seminal parameters, induction of the acrosome reaction, sperm-oocyte binding and sperm motility. As was expected no changes in the macro- and microscopically seminal parameters were caused by sildenafil citrate when compared to placebo. Furthermore the acrosome reaction was also not initiated or potentiated by sildenafil citrate at concentrations of 50mg orally. Sperm-oocyte binding, smooth path velocity, straight line velocity and the percentage rapid cells all increased after sildenafil citrate treatment. From these results it appear that there are various role players in the zona pellucidainduced acrosome reaction intracellular signalling system. A thorough understanding of such signal transduction systems and the crosstalk in-between will ultimately yield information regarding the nature of receptors to which these signal transduction pathways are coupled in human spermatozoa as well as the intracellular effectors that ultimately regulate sperm function. Moreover, an understanding of these regulatory pathways will be essential for the future development of clinical approaches designed to enhance or preclude fertilization.
AFRIKAANSE OPSOMMING: Die outeur lê in hierdie studie nuwe data voor ten einde meer lig te werp op die relatief vae veld van intrasellulêre seintransduksie paaie betrokke by die fisiologiesgeïnduseerde akrosoomreaksie en die daaropvolgende gebeure wat aanleiding gee tot bevrugting. Die zona pellucida-geïnduseerde akrosoomreaksie, sperm-zona interaksie sowel as spermmotiliteitseienskappe is ondersoek. Die eerste gedeelte van die studie fokus op die rol van ekstrasellulêreseingereguleerde- kinase (ERK), 'n lid van die familie van mitogeen-geaktiveerde proteïenkinases, tydens die zona pellucida-geïnduseerde akrosoomreaksie en sperm-oosiet interaksie. Daar word aangetoon dat die inhibisie van ERK die zona pellucida geïnduseerde akrosoomreaksie, soos gemeet met behulp van fluorosensie mikroskopie, betekenisvol verminder. Dit suggereer dat ERK direk of indirek betrokke is by die seintransduksie paaie waardeur die akrosoomreaksie van die menslike sperm deur die zona pellucida geïnduseer word. In 'n poging om onomwonde te bewys dat ERK betrokke is by menslike akrosoom-seintransduksie, is hemizona essais gebruik om sperm-oesiet binding te bepaal. Van hierdie sperm-oosiet bindingeksperimente is dit duidelik dat die inhibisie van ERK aanleiding gee tot verhoogde binding. Hierdie resultate ondersteun dus die idee dat die zona pellucidageïnduseerde akrosoomreaksie en dus die fisiologies relevante eksositotiese gebeurtenis gereguleer word deur 'n ERK-gemediëerde seintransduksie proses. In die tweede gedeelte van die studie is die belang van fosfatidielinositol 3-kinase (PI3K) in die proses van menslike spermmotiliteit, akrosoomreaksie en sperm-oesiet binding ondersoek deur van die spesifieke PI3K inhibitor LY294002, gebruik te maak. Pl3K-inhibisie het die persentasie motiliteit en progressiewe motiliteit by astenozoospermiese pasiënte verhoog. Onder hierdie laboratoriumtoestande het Pl3K-inhibisie nie die akrosoomreaksie beïnvloed nie, terwyl sperm-oosiet binding verhoog is. Hierdie resultate beteken dus dat PI3K spermmotiliteit en zona-binding negatief beïnvloed. In die laaste gedeelte van die studie is die effekte van intrasllulêre cGMP akkumulasie deur akute in vivo sildenafil sitraat (ViagraTM) toediening op seminale parameters, induksie van die akrosoomreaksie, sperm-oesiet binding en spermmotiliteit ondersoek. Soos verwag is geen veranderinge in die makro- en mikroskopiese seminale parameters deur sildenafil sitraat in vergelyking met plasebo veroorsaak nie. Verder is die akrosoomreaksies ook nie deur 50mg orale sildenafil sitraat geïnisieer of potensieer nie. Sperm-oosiet binding, geplaneerde snelheid, reguitlyn snelheid en persentasie vinnigbewegende selle was almal vehoog na sildenafil sitraat behandeling. Uit hierdie resultate blyk dit dat daar verskeie rolspelers in die zona pellucidageïnduseerde akrosoomreaksie is. 'n Deeglike insig van al hierdie seintransduksiepaaie en die onderlinge kruiskontak tussen mekaar sal uiteindelik die nodige inligting rakende die reseptore waaraan hierdie seintransduksie paaie gekoppel is, verskaf sowel as die intrasellulêre effektore wat uiteindelik spermfunksie beheer. Nog te meer sal die begrip van hierdie regulatoriese paaie verder noodsaaklik wees vir die toekomstige ontwikkeling van kliniese benaderings om bevrugting te bevorder of te voorkom.
Fernandes, Isabelle. "Etude fonctionnelle des protéines à domaine Zona Pellucida au cours de la morphogenèse épidermique embryonnaire chez la Drosophile". Toulouse 3, 2009. http://thesesups.ups-tlse.fr/767/.
Pełny tekst źródłaThe Zona Pellucida Domain (ZPD) defines a conserved family of membrane-anchored matrix proteins that are as yet poorly characterized with respect to their functions during development. Using genetic approaches in flies, we show here that a set of 8 ZPD proteins is required for the localized reorganization of embryonic epidermal cells during morphogenesis. Despite varying degrees of sequence conservation, these ZPD proteins exert specific and non-redundant functions in the remodeling of epidermal cell shape. Each one accumulates in restricted sub-regions of the apical compartment, where it organizes local interactions between the membrane and the extracellular matrix. In addition, ZPD proteins are required to sculpture the actin-rich cell extensions and maintain appropriate polarization of the apical compartment. These results on ZPD proteins therefore reveal a functional sub-compartmentalization of the apical membrane and its role in the polarized control of epithelial cell shape during development
Dietzel, Eileen [Verfasser]. "The biological role of fetuin-B : inhibition of zona pellucida hardening and preservation of female fertility in mice / Eileen Dietzel". Aachen : Hochschulbibliothek der Rheinisch-Westfälischen Technischen Hochschule Aachen, 2015. http://d-nb.info/1066813701/34.
Pełny tekst źródłaStröm, Holst Bodil. "In vitro characterisation of cryopreserved canine spermatozoa : with special reference to post-thaw survival time and zona pellucida binding capacity /". Uppsala : Swedish Univ. of Agricultural Sciences (Sveriges lantbruksuniv.), 1999. http://epsilon.slu.se/avh/1999/91-576-5445-X.pdf.
Pełny tekst źródłaPetersen, Claudia Guilhermino [UNESP]. "Correlação da maturação e da morfologia do oócito humano com as características do fuso meiótico e da zona pelúcida". Universidade Estadual Paulista (UNESP), 2009. http://hdl.handle.net/11449/106388.
Pełny tekst źródłaNas últimas décadas, alguns parâmetros laboratoriais têm sido propostos para identificar o potencial de saúde oocitária em Reprodução Assistida: 1. Maturidade nuclear e citoplasmática (pesquisa); 2. Dismorfismo (anomalias intracitoplasmáticas); 3. Fertilizaçao. Habitualmente, esses parâmetros (exceto a maturação citoplasmática) são avaliados pelo simples emprego de sistemas de microscopia de luz convencional. Nos últimos oito anos, foram relatados diversos estudos em oócitos humanos sobre a birrefringência da zona pelúcida (BF-ZP), a espessura da ZP, e a visualização do fuso meiótico (FM). Entretanto, ainda é discutível o poder desses novos parâmetros na seleção de óvulos com o pontecial de originar embriões com taxas de implantação elevadas, além disso, seu sistema de visualização e quantificação é complexo e dispendioso. O objetivo deste estudo foi investigar uma possível correlação entre esses parâmetros tradicionais com a BF-ZP, espessura da ZP e o FM. Dessa forma, um total de 73 pacientes que participaram do programa de injeção intracitoplasmática de espermatozóide (ICSI) no Centro de Reprodução Humana “Prof Franco Júnior” tiveram seus óvulos avaliados quanto a maturação nuclear (n=83), maturação citoplasmática (n=29), dismorfismo (n=280) e fertilização (n=216) sendo os resultados correlacionados com a imagem do FM (presença ou ausência), a intensidade da BF-ZP (positiva ou negativa) e a espessura da ZP. Os dados finais revelaram uma fraca correlação entre a maturação, o dismorfismo e a fertilização oocitária com a imagem do FM, a intensidade da BF-ZP, e a espessura da ZP. Entretanto, esses parâmetros poderiam ser responsáveis por identificar pontos biológicos diferentes do potencial de qualidade oocitária. Assim sendo, seria uma medida coerente, a utilização desses parâmetros, em forma conjunta, através de um escore de qualidade oocitária.
During the last decades, three laboratorial parameters have been proposed to identify the potential of the oocyte quality in ART: 1- Nuclear and cytoplasmic maturation; 2-Dysmorphims (intracytoplasmic abnormalities); 3 - Fertilization. These parameters (except the cytoplasmic maturation) are assessed by a simple system of conventional light microscopy. In the last eight years, numerous studies about zona pellucida birefringence (BF-ZP), zona pellucida thickness and meiotic spindle visualization have been reported. However, it is still controversial the power of these parameters in selecting oocyte with potential to develop into implantation-competent embryos. Furthermore, the microscopy system is complex and expensive. The present study aims to evaluate whether there is any correlation between the classical parameters with BF-ZP, ZP thickness and spindle image. A total of 73 patients who participated in an ICSI program had their oocytes evaluated such nuclear maturation (n=83), cytoplasmic maturation (n=29), dysmorphism (n=280) and fertilization (n=216) being its results correlated to the spindle image (presence or absence), BF-ZP intensity (positive or negative) and ZP thickness. The final results showed a low correlation between maturation, dysmorphism and fertilization compared to spindle image and ZP characteristics (BF-ZP, ZP thickness). However, these factors can be responsible to identify specific biological differ from the oocyte potential. Concluding, the use of these factors in association, as a score (oocyte quality score), could be the correct evaluation.
Safatli, Micheline. "Synthesis of neutral and acidic ß-O-linked glycoconjugates related to those found in the core structure of mammalian zona pellucida". Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1998. http://www.collectionscanada.ca/obj/s4/f2/dsk2/ftp03/MQ36522.pdf.
Pełny tekst źródłaGrullón, Yunén Luis Alberto. "Función del sistema plasminógeno-plasmina en la fecundación de ovocitos bovinos y porcinos". Doctoral thesis, Universidad de Murcia, 2010. http://hdl.handle.net/10803/32113.
Pełny tekst źródłaThe aim of this study was to describe the role of the plasminogen/plasmin system (PLG/PLA) in bovine and porcine fertilization. Through in vitro fertilization, we demonstrated that the presence of PLG or PLA in the incubation medium of gametes decreased penetration of oocytes and sperm binding to the zona pellucida (ZP). This decrease was not due to alterations in sperm function or changes in the ZP resistance to proteolysis, but the PLA caused the release of sperm previously bound to the ZP. By indirect immunofluorescence we detected the presence of PLG and its activators in the ZP and oolema of the oocytes before fertilization. After fertilization, this presence diminished or disappeared completely, so we propose that the PLG/PLA system is activated during sperm-oocyte interaction and contributes to the regulation of polyspermy.
El-Mestrah, Majid Fouad. "Zona pellucida glycoproteins, structural topography and modifications of the carbohydrate determinants during folliculogenesis and following in vivo fertilization and in vitro egg activation". Thesis, National Library of Canada = Bibliothèque nationale du Canada, 2001. http://www.collectionscanada.ca/obj/s4/f2/dsk3/ftp04/NQ59525.pdf.
Pełny tekst źródłaHermansson, Ulrika. "Studies of canine and feline sperm viability under different storage procedures : with special reference to chilling, freezing, and use of zona pellucida binding assays /". Uppsala : Department of Clinical Sciences, Swedish University of Agricultural Sciences, 2006. http://epsilon.slu.se/200668.pdf.
Pełny tekst źródłaHanna, William F. "Sperm-zona pellucida interactions in the mouse evidence that Lewis X-containing neoglycoproteins mimic the intrinsic ability of ZP3 to induce the acrosome reaction /". Available to US Hopkins community, 2002. http://wwwlib.umi.com/dissertations/dlnow/3068781.
Pełny tekst źródłaSaucedo, Manuel Nicolas Verfasser], i Eckhard [Akademischer Betreuer] [Wolf. "Comparison between two vitrification methods for in vitro produced bovine embryos with an intact or a nonintact zona pellucida / Manuel Nicolas Saucedo. Betreuer: Eckhard Wolf". München : Universitätsbibliothek der Ludwig-Maximilians-Universität, 2016. http://d-nb.info/1102157201/34.
Pełny tekst źródłaBou, Khalil Maroun. "Biophysical and biochemical properties of the mammalian male germ cell specific sulfogalactosylglycerolipid (SGG): Contribution to the structure and zona pellucida affinity of pig sperm raft membranes". Thesis, University of Ottawa (Canada), 2004. http://hdl.handle.net/10393/29079.
Pełny tekst źródłaZhang, Bing Rong. "Evaluation of frozen-thawed semen from Swedish Red and White AI bulls : with special reference to the relation between zona pellucida binding, in vitro fertilization and in vivo fertility /". Uppsala : Swedish Univ. of Agricultural Sciences (Sveriges lantbruksuniv.), 1998. http://epsilon.slu.se/avh/1998/91-576-5436-0.gif.
Pełny tekst źródłaBulgarelli, Daiane Lopes. "A vitrificação de oócitos bovinos prejudica sua capacidade reprodutiva, independente do estadio de maturação". Universidade de São Paulo, 2011. http://www.teses.usp.br/teses/disponiveis/17/17145/tde-10012017-115656/.
Pełny tekst źródłaUntil the present literature has not achieved a consensus regarding the best maturation stage for oocyte to maintain their reproductive capacity after cryopreservation. The aim of this study was to determine, using an experimental bovine model, in which stage of development (VG stage, immature, or MII stage, post-maturation in vitro) the oocyte is less susceptible to damage during cryopreservation. Immature oocytes (VG) from the ovaries of slaughtered cows were selected for in vitro maturation or vitrification and divided into three groups. The first group (CONTROL) consisted of immature oocytes, matured in vitro without vitrification; the second group (CRYO-IVM) consisted of vitrified immature oocytes thawed and submitted to in vitro maturation; and the third group (IVM-CRYO) consisted of matured in vitro oocytes submitted to vitrification and thawing. The oocytes were evaluated for: nuclear maturation by acetic orcein staining; integrity of the zona pellucida using a polarized microscope; cell viability by the Dead-Live technique; and embryo development (cleavage, production and hatching rate) by in vitro fertilization and parthenogenetic activation. There was no difference in capacity of nuclear maturation between fresh and thawed oocytes (p=0.23). Regarding the zona pellucida (ZP), all oocytes (100%) of all three groups (control, CRYO-IVM and IVMCRYO) presented a positive ZP reading, with no correlation with later embryo evolution. DEAD-LIVE analysis of cell viability revealed reduction of viability in the IVM-CRYO group (27%) compared to control (84%) (p<0.0001) and to the CRYO-IVM group (56%) (p=0.017), with no difference between the last two groups (p=0.055). Analysis of the potential for embryo development by means of in vitro fertilization showed that the control group presented better cleavage and blastocyst formation rates than the CRYO-IVM (p<0.0001 and p<0.0001, respectively) and the IVM-CRYO (p<0.0001 and p=0.0004, respectively) groups. Analyzing the potential for embryo development the control group presented better cleavage by means of in vitro fertilization (80%) and parthenogenetic activation (58%) than the CRYOIVM (28%; p<0,0001; 28%; p=0,0002, respectively) and the IVM-CRYO groups (26%; p<0,0001; 22%, p<0,0001,respectively) Analysis of blastocyst formation rates and hatching after FIV and AT in CRYO-IVM and IVM-CRYO groups were null. Vitrification of bovine oocytes causes great impairment of their reproductive capacity regardless of the stage of maturation at the time of freezing. However the vitrified immature oocytes submitted to IVM maintained their capacity of nuclear maturation, as they achieved MII stage. This study was not able to determine which stage was better in reducing crio damage, as both stages (VG and MII) presented equally impaired by the process.
Cánovas, Bernabé Sebastián. "Interacciones homólogas y heterólogas in vitro de gametos porcinos, bovinos y humanos y sus aplicaciones en el estudio de la fecundación". Doctoral thesis, Universidad de Murcia, 2007. http://hdl.handle.net/10803/10851.
Pełny tekst źródłaThe interaction between gametes is crucial to fertilization. The zona pellucida (ZP) is responsible to block of polyspermy, but in vitro these functions are not efficient. The polyspermy is frequently in bovine and porcine in vitro fecundation. Besides the heterologous interaction between spermatozoa-oocyte had been described. The aims were study the block of polyspermy to improve the output of IVF and research the heterologous interactions between human spermatozoa and porcine oocyte.The results show that there is hardening of bovine and porcine ZP previously at fertilization, in vivo and using DTSP or bovine oviductal fluid. When these oocytes are used in IVF improve the monospermy and the output. In heterologus interactions the human spermatozoa could bind to porcine ZP and it triggers the acrosome reaction, but not penetration in ZP-free oocyte was observed. In ICSI the oocyte activation and
Kongmanas, Kessiri. "Roles of Seminolipid and Its Associated Membrane Domain in Male Fertility". Thesis, Université d'Ottawa / University of Ottawa, 2015. http://hdl.handle.net/10393/32509.
Pełny tekst źródłaMazzeo, Ilaria. "Effect of thermal regime on the expression of key reproductive genes during hormonally-induced vitellogenesis in female European eels". Doctoral thesis, Universitat Politècnica de València, 2015. http://hdl.handle.net/10251/48490.
Pełny tekst źródłaMazzeo, I. (2014). Effect of thermal regime on the expression of key reproductive genes during hormonally-induced vitellogenesis in female European eels [Tesis doctoral no publicada]. Universitat Politècnica de València. https://doi.org/10.4995/Thesis/10251/48490
TESIS
Busca, Cláudia Sofia Nunes. "Analysis of the role of ZP3 and APP in cell-cell adhesion". Master's thesis, Universidade de Aveiro, 2014. http://hdl.handle.net/10773/12945.
Pełny tekst źródłaAmong different types of cell-cell interactions present in the body, the interaction between the oocyte and a sperm cell is of great importance for species perpetuation. Cellular adhesion is thus a crucial process in fertilization, which involves the oocyte’s zona pellucida (ZP), the first barrier found by sperm cells that mediates the first contact between gametes. ZP3 is a major ZP glycoprotein of the oocyte, and its ligand in sperm cells is still unknown. The APP glycoprotein was found by us to be in the sperm’s head, including its equatorial zone involved in sperm-oocyte interaction. This protein plays a role in the pathogenesis of the Alzheimer’s disease and its physiologic roles are still being unravelled. The present work aimed to characterize a putative APP-ZP3 interaction in cell-cell adhesion. Relevant functional motifs on the ZP3 amino acid sequence were analysed, and the role of ZP3 and APP in cell-cell adhesion was evaluated. Interference assays were performed with antibodies against epitopes of APP, ZP3 and β1-integrin, the latter an important protein in cellular adhesion known to bind APP. The subcellular co-localization of ZP3 and APP was analysed by immunocytochemistry assays, and a potential physical interaction between ZP3 and APP was evaluated by immunoprecipitation. Results indicate that, from the studied proteins, APP and β1-integrin are the most important in cell-cell adhesion, probably through a common pathway. ZP3 has only a minor effect in cell-cell adhesion, but is able to interfere with adhesion mediated by APP and β1-integrin, potentially sharing their common pathway. Surprisingly, ZP3 and APP only co-localize at low quantities in vesicles, mainly near the Golgi apparatus, while there is a slightly higher degree of co-localization between ZP3 and β1-integrin near the plasma membrane. Unexpectedly though, ZP3 and APP were found to co-localize in extracellular aggregates of secreted ZP3. Immunoprecipitation results so far further suggest that highly glycosylated ZP3 and APP forms physically interact, what may reflect an important interaction between these proteins in the oocyte’s ZP upon fertilization.
Entre os diversos tipos de interações entre células que ocorrem no organismo, a interação entre o oócito e o espermatozoide é de grande importância para a perpetuação da espécie. A adesão celular é então um processo fundamental para a fertilização, a qual envolve a zona pelúcida (ZP) dos oócitos, a primeira barreira encontrada pelos espermatozoides que medeia a primeira interação entre os gâmetas. A ZP3 é uma das principais proteínas da ZP, e ainda não se conhece qual o seu ligando no espermatozoide. A glicoproteína APP foi descrita por nós como estando presente na cabeça do espermatozoide, em particular na zona equatorial, envolvida na interação espermatozoide-oócito. Esta proteína é central à patogénese da Doença de Alzheimer e os seus papéis fisiológicos ainda não estão bem caracterizados. Este trabalho focou-se na caracterização de um possível interação entre a APP e a ZP3 na adesão célula-célula. Foi realizado um levantamento de motivos funcionais relevantes no ZP3, e avaliado o papel deste e da APP na adesão célula-célula. Para tal foram realizados ensaios de interferência com anticorpos contra epitopos da APP, da ZP3, e também da β1-integrina, um proteína importante na adesão celular e que se sabe ligar à APP. A co-localização subcelular entre a ZP3 e a APP foi analisada por imunocitoquímica, e uma possível interação física entre a ZP3 e APP monitorizada através de ensaios de imunoprecipitação. Os resultados indicam que, de entre as proteínas estudadas, a APP e a β1-integrina são as mais importantes na adesão celular, provavelmente através de uma via comum. A ZP3 parece ter apenas um pequeno efeito na adesão célula-célula, possivelmente através da mesma via da APP e da β1-integrina. Surpreendentemente, ZP3 e APP apresentam pouca co-localização, e especialmente em vesiculas perto do complexo de Golgi. Contudo, existe um maior grau de co-localização entre a ZP3 e a β1-integrina, particularmente perto da membrana plasmática. Inesperada foi também a presença de agregados extracelulares de ZP3 secretada contendo também APP. Os resultados preliminares dos ensaios de imunoprecipitação sugerem uma interacção física entre as formas altamente glicosiladas da APP e da ZP3, o que pode refletir uma importante interação destas duas proteínas na ZP do oócito aquando da fertilização.
LUCAS, HERVE. "La zone pellucide : etude des oligosaccharides dans deux especes animales et chez l'homme". Angers, 1993. http://www.theses.fr/1993ANGE1130.
Pełny tekst źródłaBercegeay, Sylvain. "Contribution a l'etude de la zone pellucide de l'ovocyte : composition et modification au cours du processus de fecondation". Nantes, 1995. http://www.theses.fr/1995NANT01VS.
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