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Rozprawy doktorskie na temat „Virus Cell Fusion”

Autor: Grafiati

Data publikacji: 7 września 2023

Data aktualizacji: 31 lipca 2025

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1

Barkley, Russell. "Investigation of an Oncolytic MeV Cell-Cell Fusion Phenomenon Induced by an siRNA." Thesis, Université d'Ottawa / University of Ottawa, 2020. http://hdl.handle.net/10393/41531.

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Oncolytic measles virus is a promising cancer therapeutic in clinical trials which possesses multiple characteristics that are advantageous over traditional therapies. Currently, clinical oncolytic measles virus vectors are unmodified or express reporter transgenes that benefit its therapeutic efficacy. The next phase in its development will see genetically engineered vectors encoding transgenes that enhance its antineoplastic effects. To this end, preclinical research has focused on studying novel transgenes which favour viral replication, cytotoxicity, and the anti-cancer immune response

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Garg, Himanshu. "Feline Immunodeficiency Virus (FIV) Envelope Glycoprotein-Mediated Cell Fusion and Apoptosis." NCSU, 2003. http://www.lib.ncsu.edu/theses/available/etd-11042003-141554/.

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Feline Immunodeficiency Virus (FIV) and Human Immunodeficiency Virus (HIV) are lentiviruses that are remarkable similar in their genomic organization, receptor usage and pathogenesis. Based on this FIV has evolved into a well-established small animal model for studying AIDS. FIV and HIV cause a progressive depletion of T cells via a still unknown mechanism though numerous studies support a role of membrane expressed HIV env glycoprotein in apoptotic killing of CD4+ T cells. HIV env glycoprotein is a heterodimer of surface expressed gp120 that binds to CD4 and a chemokine receptor and transmemb

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Bickerton, Erica Jane. "Cellular tropism and cell-to-cell fusion properties of the infectious bronchitis virus spike glycoprotein." Thesis, University of Warwick, 2010. http://wrap.warwick.ac.uk/35165/.

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There are numerous vaccines available for the control of infectious bronchitis virus (IBV) in poultry, however protection is short-lived and poorly cross-protective between strains. The vaccines must currently be grown in embryonated eggs, a cumbersome and expensive process. The ability to grow vaccines on a cell-line such as Vero cells would be highly advantageous. The spike (S) glycoprotein of IBV is comprised of two subunits, S1 and S2, has a vital role in virulence in vivo and is responsible for cellular tropism in vitro. This project aims to identify the amino acids present in the S glyco

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Symeonides, Menelaos. "HIV-1-Induced Cell-Cell Fusion: Host Regulation And Consequences For Viral Spread." ScholarWorks @ UVM, 2016. https://scholarworks.uvm.edu/graddis/589.

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Human immunodeficiency virus type 1 (HIV-1) is a human retrovirus of the lentivirus subgroup which primarily infects T cells and macrophages, and causes acquired immune deficiency syndrome (AIDS). Since its emergence in the early 1980s, HIV-1 has caused a global pandemic which is still responsible for over one million deaths per year, primarily in sub-Saharan Africa. HIV-1 has been the subject of intense study for over three decades, which has resulted not only in major advances in cell biology, but also in numerous drug treatments that effectively control the infection. However, cessation of

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Leung, Sze-Yui Horasis, and 梁思睿. "Fibronectin: role in viral cell association, fusion and entry of influenza A virus." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2012. http://hub.hku.hk/bib/B48329708.

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The influenza A viral hemagglutinin (HA) protein binds to sialic acid (SA) groups of cellular surface glycoproteins to achieve viral attachment and entry. The SA binding specificity of HA is one of the major determinants for controlling viral tropism and host specificity. Fibronectin (FN) is a ubiquitinious glycoprotein secreted on cell surface, either circulating in plasma, or as one of the best characterized components of the extra cellular matrix. With its binding properties towards different types of molecules and pathogens, it has been utilized by different bacterial and viral pathoge

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Wagenaar, Timothy Robert. "Regulation of infected cell fusion by the vaccinia virus A56 and K2 proteins." College Park, Md.: University of Maryland, 2008. http://hdl.handle.net/1903/8044.

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Thesis (Ph. D.) -- University of Maryland, College Park, 2008.<br>Thesis research directed by: Dept. of Cell Biology & Molecular Genetics. Title from t.p. of PDF. Includes bibliographical references. Published by UMI Dissertation Services, Ann Arbor, Mich. Also available in paper.

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Hummel, Kimberly Brown. "Alteration of the measles virus glycoproteins as a mechanism to reduce cell fusion during persistence." Diss., Georgia Institute of Technology, 1994. http://hdl.handle.net/1853/25597.

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Hutchinson, Lloyd M. "Glycoprotein K of herpes simplex virus (HSV), role in viral egress and HSV-induced cell-cell fusion." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1997. http://www.collectionscanada.ca/obj/s4/f2/dsk2/tape16/PQDD_0016/NQ30094.pdf.

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Al-Torki, Reem. "Mapping of B-cell epitopes on the fusion protein of human respiratory syncytial virus." Thesis, London School of Hygiene and Tropical Medicine (University of London), 2004. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.415976.

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Marques, Sandra Eugénia Leite. "Expressão em Escherichia coli de antigénios do Cell fusing agent virus (Flaviviridae: Flavivirus) como proteína de fusão." Master's thesis, Faculdade de Ciências Médicas. UNL, 2012. http://hdl.handle.net/10362/8531.

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RESUMO: O Cell Fusing Agent Vírus (CFAV), considerado como o primeiro “flavivírus específicos de insectos” (ISF), parece estar exclusivamente adaptado aos seus hospedeiros, não replicando em células de vertebrados. Apesar de ter sido identificado há mais de três décadas (1975), a verdade é que muito pouco se conhece sobre a sua biologia. Dado o seu parentesco filogenético com alguns outros flavivírus encontrados naturalmente em mosquitos de diferentes géneros colhidos em diferentes regiões do globo, este vírus poderá ser usado como modelo para o estudo de ISF. No entanto, necessitam do

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Gerald, Schneikart. "Respiratory syncytial virus fusion protein-specific B cell repertoires induced by natural infection or vaccination." Doctoral thesis, Università di Siena, 2018. http://hdl.handle.net/11365/1050834.

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Respiratory syncytial virus (RSV) infections are the major contributor to acute lower respiratory syndrome in newborns. Infections generally result in hospitalization and sometimes in death. A vaccine is not available yet, despite decades of research. Vaccine development is hampered in consequence of a failed vaccine trial in the 1960s which entailed fatal outcomes. An alternative to direct vaccination of children is maternal vaccination for passive immunization of babies before birth. RSV infects every person repeatedly throughout life which implies that pregnant woman carry RSV-directed memo

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Whyte, Paul. "Identification and characterisation of protective B cell epitopes on the fusion protein of respiratory syncytial virus." Thesis, University of Reading, 1994. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.241353.

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Conner, Rebecca. "The Effects of Adjacent Cell Fusion and Ultraviolet Radiation Exposure on Viral Plaque Formation with Herpes Simplex Virus Type I." TopSCHOLAR®, 1986. http://digitalcommons.wku.edu/theses/1906.

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In mammalian cell-virus systems, it has been observed that damage caused by exposure of the cell to ultraviolet radiation (UV) will result in an increase in viral plaque development rate. This phenomenon is termed the Large Plaque Effect (LPE). Apparently, viral plaque development increases at a faster rate for Herpes Simplex Virus (HSV) when it is assayed on certain UV-irradiated mammalian cells. The consequence of this increase in plaque development rate is that viral plaques appear larger on irradiated monolayers of cells when compared to plaques that developed on unirradiated cellular mono

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Matheny, Elizabeth Lane. "Contribution of the membrane-proximal region of the vesicular stomatitis virus gycoprotein to host cell entry and membrane fusion." View the abstract Download the full-text PDF version, 2009. http://etd.utmem.edu/ABSTRACTS/2009-043-Matheny-index.htm.

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Thesis (Ph.D.)--University of Tennessee Health Science Center, 2009.<br>Title from title page screen (viewed on February 3, 2010). Research advisor: Michael A. Whitt, Ph.D. Document formatted into pages (x, 91 p. : ill.). Vita. Abstract. Includes bibliographical references (p. 81-90).

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Ke, Lijing. "Mechanism of anti-influenza virus activity of Maillard reaction products derived from Isatidis roots." Thesis, University of Edinburgh, 2011. http://hdl.handle.net/1842/6501.

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The cyto-protective compositions and effects of antiviral Maillard reaction products (MRPs) derived from roots of Isatis indigotica F. were examined using biochemical and biophysical methods. The Maillard reaction was identified as the main source of compounds with antiviral activity, an observation which has led to the proposal of a new class of active compounds that protect cells from influenza virus infection. In the roots, arginine and glucose were revealed to be the predominant reactants for the Maillard reaction. Significant anti-influenza virus effects were demonstrated in the RIE MRPs

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Boutin, Elodie. "Mécanisme d'inhibition de la fusion membranaire du virus de l'hépatite C par différents composés : l'arbidol, la silymarine et les molécules la composant." Thesis, Lyon 1, 2010. http://www.theses.fr/2010LYO10244.

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L'infection par le virus de l'hépatite C (VHC) est un problème de santé publique majeur car en absence de vaccin et de thérapie suffisamment efficace, l’infection peut dégénérer en carcinome hépatocellulaire. Il est alors important d’identifier de nouvelles cibles thérapeutiques et de développer de nouveaux antiviraux. Ainsi, nous avons étudié l'activité anti-VHC de différents composés : l'arbidol (Arb), la silymarine (SM) et les molécules la composant, notamment la silibinine (SbN). Ces composés ont l'avantage d'être déjà utilisés en médecine humaine depuis de nombreuses années et ont ainsi p

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Andersson, Christin. "Production and delivery of recombinant subunit vaccines." Doctoral thesis, KTH, Biotechnology, 2000. http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-3027.

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<p>Recombinant strategies are today dominating in thedevelopment of modern subunit vaccines. This thesis describesstrategies for the production and recovery of protein subunitimmunogens, and how genetic design of the expression vectorscan be used to adapt the immunogens for incorporation intoadjuvant systems. In addition, different strategies fordelivery of subunit vaccines by RNA or DNA immunization havebeen investigated.</p><p>Attempts to create general production strategies forrecombinant protein immunogens in such a way that these areadapted for association with an adjuvant formulation wer

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Corey, Elizabeth Ann. "Characterization of the Relationship Between Measles Virus Fusion, Receptor Binding, and the Virus-Specific Interaction Between the Hemagglutinin and Fusion Glycoproteins: a Dissertation." eScholarship@UMMS, 2006. https://escholarship.umassmed.edu/gsbs_diss/221.

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Measles (MV) virions, like those of other enveloped viruses, enter cells by fusing their lipid membranes with those of the target host cells. Additionally, infected tissues often possess giant multinucleate cells, known as syncytia, which are formed by fusion of infected cells with uninfected neighbors. Expression of both the MV attachment (H) and fusion (F) proteins is required for membrane fusion. MV H mediates receptor binding in order to bring the two membranes into close proximity prior to F activation and is thought to trigger F activation through a specific interaction between the two p

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Wychowski, Czeslaw. "Expression de la proteine de capside vp1 du poliovirus dans les bacteries et dans les cellules animales : identification d'un epitope de neutralisation et caracterisation de sequences indispensables a l'accumulation de proteines dans le noyau." Paris 7, 1987. http://www.theses.fr/1987PA077173.

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Tam, John. "Expression of the membrane fusion protein of measles virus in insect and mammalian cells using recombinant viruses." Thesis, McGill University, 1993. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=69521.

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The fusion (F) protein of measles virus is responsible for viral penetration at the plasma membrane and syncytia formation. In order to facilitate future structure/function studies, the F protein was expressed in insect cells using recombinant baculovirus, and in mammalian cells using both vaccinia and adenovirus recombinants. The baculovirus system exhibited the highest levels of recombinant protein expression, but the majority of the product was found to be in the form of detergent-insoluble precursors. Replacing the signal peptide of the F protein with insect-derived sequences did not enhan

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Krishna, Benjamin Anthony Cates. "Investigating and exploiting the latency-associated expression of the human cytomegalovirus gene US28 in early myeloid lineage cells." Thesis, University of Cambridge, 2017. https://www.repository.cam.ac.uk/handle/1810/267737.

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Human cytomegalovirus (HCMV) is a betaherpesvirus which establishes a lifelong persistent infection, underpinned by its ability to establish latent infection in early myeloid lineage cells, in the infected host. Although well controlled by a healthy immune system, HCMV causes pathological and life threatening disease in individuals with a compromised or immature immune response, which can come from primary HCMV infection or reactivation of latent infection. Although progress is being made in understanding the mechanisms by which HCMV maintains latency and reactivates, a better understanding is

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22

Krüger, Nadine [Verfasser]. "Interaction of bat-derived paramyxoviruses with chiropteran and non-chiropteran cells: Functional characterization of the African henipavirus and bat-derived mumps virus fusion and attachment glycoproteins / Nadine Krüger." Hannover : Bibliothek der Tierärztlichen Hochschule Hannover, 2014. http://d-nb.info/106520874X/34.

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Baidaliuk, Artem. "Interactions between the insect-specific flavivirus CFAV, its mosquito host aedes aegypti, and co-infecting arboviruses." Electronic Thesis or Diss., Sorbonne université, 2019. https://accesdistant.sorbonne-universite.fr/login?url=https://theses-intra.sorbonne-universite.fr/2019SORUS494.pdf.

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Les virus de la dengue (DENV) et du Zika (ZIKV) sont des virus transmis par les moustiques qui causent de graves problèmes de santé publique à travers le monde. Ces virus transmis par des arthropodes (arbovirus) sont des virus à ARN du genre Flavivirus principalement transmis à l'homme par le moustique Aedes aegypti. De plus, les moustiques Ae. aegypti sont naturellement infectés par le cell-fusing agent virus (CFAV), le premier flavivirus spécifique d'insecte (FSI) ayant été décrit. L'évolution du CFAV, ses interactions avec les arbovirus chez les moustiques co-infectés et les réponses immuni

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Das, Provas. "Role of Nonmuscle Myosin II in Virus-Cell Fusion." Thesis, 2019. http://hdl.handle.net/10821/8233.

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Membrane fusion is the process whereby two separate lipid bilayers merge to become one. Despite substantial progress, an integrated concept for protein–mediated membrane fusion (cellular and viral) is not yet available, and many open questions yet to be answered. Membrane fusion, the merging/intermixing of two lipid bilayers, is quite a well known process involved in a number of physiological functions e.g. fertilization, cell division, myoblast differentiation, transport of impermeant molecules into the cell (endocytosis) and out of the cell (exocytosis). Among all the cases of membrane fusio

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Tsai, Chang-wu, and 蔡倉吾. "Molecular Mechanisms of the Mouse Hepatitis Virus S Protein Involved in the Cell-Cell Fusion." Thesis, 1999. http://ndltd.ncl.edu.tw/handle/94630417674350045141.

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博士<br>國立臺灣大學<br>生化學研究所<br>87<br>Abstract The spike (S) glycoprotein of mouse hepatitis virus (MHV) plays a major role in the viral pathogenesis. It is often processed into the N-terminal S1 and the C-terminal S2 subunits that were evident to be important for binding to cell receptor and inducing cell-cell fusion, respectively. As a consequence of cell-cell fusion, most of naturally occurring infections of MHV are associated with syncytia formation. So far, only MHV-2 was identified to be fusion-negative. In this study, the S gene of MHV-2 was molecularly cloned and the nucleotide se

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Vaidya, Naveen K. "Membrane fusion between an influenza virus and a host cell : mathematical models /." 2008. http://gateway.proquest.com/openurl?url_ver=Z39.88-2004&res_dat=xri:pqdiss&rft_val_fmt=info:ofi/fmt:kev:mtx:dissertation&rft_dat=xri:pqdiss:NR46017.

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Thesis (Ph.D.)--York University, 2008. Graduate Programme in Mathematics and Statistics.<br>Typescript. Includes bibliographical references (leaves 166-175). Also available on the Internet. MODE OF ACCESS via web browser by entering the following URL: http://gateway.proquest.com/openurl?url_ver=Z39.88-2004&res_dat=xri:pqdiss&rft_val_fmt=info:ofi/fmt:kev:mtx:dissertation&rft_dat=xri:pqdiss:NR46017

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Liu, Kuang-Yang, and 劉光仰. "Effect of P20/GFP fusion on cell to cell movement of Bamboo mosaic virus satellite RNA." Thesis, 2012. http://ndltd.ncl.edu.tw/handle/94102388791107864334.

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碩士<br>國立中興大學<br>生物科技學研究所<br>100<br>Satellite RNAs (satRNAs) are subviral agents which depend on helper viruses and host factors for replication, encapsidation and movement. Bamboo mosaic virus (BaMV), a member of the genus Potexvirus, is the only potexvirus that naturally associates with a satRNA, designated satBaMV, which encodes a 20 kDa RNA-binding protein (P20) involved in efficient long-distance movement and replication of satBaMV. The aims of this study were to investigate the abilities of other non-cognate viruses in supporting the replication of satBaMV and to explore the effects of P2

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Mulampaka, Shiva Naresh. "Theoretical Studies of the Mechanisms of the Entry of Virus into Cells." Thesis, 2014. http://etd.iisc.ac.in/handle/2005/3082.

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Viruses cause human diseases by entering in to human cells. Many drugs have been developed that act at various stages of viral infection, but they fail due to their toxic side effects and high mutation rates of viruses. Recently, a new class of drugs called entry inhibitors has been developed which acts on the early stages of viral infection. These drugs have been developed by studying the entry process of viruses in to host cells. The success of these drugs, however, is still limited and research is being done to quantify the optimum dosage of these drugs and find new drugs targets. We develo

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Mulampaka, Shiva Naresh. "Theoretical Studies of the Mechanisms of the Entry of Virus into Cells." Thesis, 2014. http://hdl.handle.net/2005/3082.

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Viruses cause human diseases by entering in to human cells. Many drugs have been developed that act at various stages of viral infection, but they fail due to their toxic side effects and high mutation rates of viruses. Recently, a new class of drugs called entry inhibitors has been developed which acts on the early stages of viral infection. These drugs have been developed by studying the entry process of viruses in to host cells. The success of these drugs, however, is still limited and research is being done to quantify the optimum dosage of these drugs and find new drugs targets. We develo

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Tsai, Yu-Chen, and 蔡佑晨. "Expression of the Envelope Glycoprotein and Establishment of a Cell Fusion Assay of Dengue Virus." Thesis, 2001. http://ndltd.ncl.edu.tw/handle/95596026833282150742.

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碩士<br>國立臺灣大學<br>微生物學研究所<br>89<br>Dengue virus is a positive, single-stranded RNA enveloped flavivirus that is transmitted by mosquitoes, Aedes aegypti and Aedes albopictus. The virus is divided into four serotypes, DEN-1, DEN-2, DEN-3 and DEN-4. In the process of virus entry, the envelope protein, E protein, plays an important role. It is a glycoprotein of 495 amino acids, with a transmembrane domain at the C-terminal. It is believed to form a stable, non-covalently linked homodimer. The extracellular domains of E glycoprotein are thought to interact with the host cell receptor.

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Ha, Michael Neul. "In Vitro and In Vivo Studies with Measles Virus and its Interaction with the Mouse Innate Immune System." Thesis, 2012. http://hdl.handle.net/1807/32725.

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Measles is one of the most contagious diseases known to mankind. Despite the availability of a safe and effective vaccine, approximately 164,000 measles-related deaths were recorded in 2008. The inherent restricted host tropism of MV means that the development of authentic rodent models will be a valuable research tool in testing new vaccines and antivirals. In addition to the receptor requirement, mouse innate immunity has been shown to inhibit MV growth. In this thesis, the contributions of several key components of the mouse innate immune system on the inhibition of MV replication were exam

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Koslová, Anna. "Replikační bloky viru Rousova sarkomu v savčích buňkách." Doctoral thesis, 2017. http://www.nusl.cz/ntk/nusl-370879.

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One of the important tasks of virology and immunology is to explore the species- and cell-barriers preventing virus horizontal transmission and reveal the ways how viruses overcome these barriers and "adapt" to different species. This work is based on a well- established retroviral model - avian Rous sarcoma virus (RSV) and studies virus replication blocks in mammalian cells at both pre- and post-integration level. Interaction of the viral envelope glycoprotein (Env) with a specific cellular receptor mediates virus entry into cells. Although mammalian orthologues of specific chicken receptors

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Štafl, Kryštof. "Molekulární mechanismy buněčné nepermisivity vůči viru Rousova sarkomu." Master's thesis, 2017. http://www.nusl.cz/ntk/nusl-355717.

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Most viruses can infect only a reduced range of organisms and an effective replication is possible only in selected hosts. These hosts are called permissive for the virus. Molecular principles of a nonpermissiveness and viral mechanisms of overcoming replication obstacles are still not clearly elucidated. This thesis discusses the molecular causes of the cellular nonpermissiveness against a model retrovirus - Rous sarcoma virus. The research is conducted on duck cells which are semipermis- sive to the subgroup C of Rous sarcoma virus. The virus can enter those cells, but it is not able to prod

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Tseng, Ying Hsin, and 曾縈馨. "Tumorigenicity of a hepatitis B virus core gene deletion mutant encoding a core-polymerase fusion protein in hepatoma cells." Thesis, 2012. http://ndltd.ncl.edu.tw/handle/42078938731125928982.

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碩士<br>長庚大學<br>生物醫學研究所<br>100<br>Hepatitis B virus (HBV) is one of the most important human pathogens in chronic infectious diseases. Chronic hepatitis B virus infection can lead to chronic active hepatitis and liver cirrhosis, which further results in emergence of hepatocellular carcinoma. Naturally occurring mutations in HBV have been found in patients with acute or chronic HBV infection, wherein some mutants develop in certain stages of chronic HBV infection. Of these HBV mutations, core-gene-defective mutants have been found in some special patient groups. Our present study aims to investig

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Sawatsky, Bevan. "Functional characterization of the attachment glycoprotein of Nipah virus: role in fusion, inhibition of henipavirus infection, generation of chimeric proteins, and assembly of chimeric viruses." 2007. http://hdl.handle.net/1993/2809.

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Nipah virus (NiV) and Hendra virus (HeV) have been identified as the causes of outbreaks of fatal meningitis, encephalitis, and respiratory disease in Australia, Malaysia, Bangladesh, and India from 1994 until 2004. In order to accommodate the unique genomic characteristics of NiV and HeV, a new genus within the family Paramyxoviridae was created, named Henipavirus. NiV encodes two surface glycoproteins: the attachment glycoprotein (G) binds to the cellular receptor for the virus, while the fusion glycoprotein (F) mediates membrane fusion between the virus and cell membranes. Expression of F a

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