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Besarani, Dler. "Anti-Vimentin Antibodies in Renal Transplantation". Thesis, Imperial College London, 2010. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.526360.
Pełny tekst źródłaWong, Kai-lun, i 黃棨麟. "Nanomechanical studies of vimentin intermediate filaments". Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2012. http://hub.hku.hk/bib/B49799617.
Pełny tekst źródłapublished_or_final_version
Orthopaedics and Traumatology
Doctoral
Doctor of Philosophy
Carter, D. Vaughan. "The role of vimentin antibodies in transplantation". Thesis, University of Newcastle Upon Tyne, 2006. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.424154.
Pełny tekst źródłaVechio, Aluana Maria da Costa Dal. "Expressão da vimentina em cultivo tridimensional de linhagens celulares derivadas de carcinoma epidermóide de boca". Universidade de São Paulo, 2008. http://www.teses.usp.br/teses/disponiveis/23/23141/tde-08042009-160336/.
Pełny tekst źródłaHead and neck squamous cell carcinoma (HNSCC) represents more than 90% of all head and neck malignancies, causing more deaths than any other oral disease. Proteins related to cancer growth, invasion and metastasis are in evidence due to their involvement in carcinogens, such as vimentin. This protein is observed in mesenquimal cells, however, it is considered a common finding in cervix, breast and bladder tumours. Thus its presencence in epithelial neoplasic cells contributes to epithelial-mesenchymal transition associated with tumorigenesis and tumor progression. The aim of this study was to analyse through Western Blot, Immunohistochemistry and Immunofluorescence methods, the expression of Vimentin in three different HNSCC cell lines and HaCat cell line (immortalized keratinocytes) submitted to a 3D assay into Matrigel®. The control group was represented by the same cell lines, without any treatment. Results showed that Vimentin had citoplasmatic staining in some cell of lines studied, except for HaCat cells, with evident decrease in its expression when submitted to cultive into Matrigel®. These findings were confirmed by Western Blot. Taking these results together, we conclude that in squamous cell carcinoma, the Vimentin is related to the process of tumour invasion and metastasis. This fact was showed by the reduction of its expression after treatment with Matrigel®. Therefore, the expression of Vimentin in different cell lines of HNSCC may vary according to the stimulus and, fundamentally, the localization of the tumor and the individual characteristics of neoplasic cells.
Gianelo, Maikol Carlos Simões. "Estudo da resposta regenerativa do músculo sóleo de ratas bebês após procedimento de imobilização e reabilitação pelo alongamento". Universidade de São Paulo, 2014. http://www.teses.usp.br/teses/disponiveis/17/17152/tde-02072015-122858/.
Pełny tekst źródłaDisuses models of skeletal muscle as immobilization , suspension are often used in experimental research groups. This time of disuse for a prolonged period can cause significant changes in muscle cytoarchitecture .This study aimed to evaluate the morphology of the soleus muscle of rats in postnatal development that had its members later immobilized rights, and subsequently were subjected to passive stretching protocol (passive manual stretching flashes), for a period of seven days. We used 20 Wistar rats (Rattus Norvegicus Albinos) race with 21 days of age , divided into five groups: Control Group (CG21- Animal 21 days ), Immobilized Group (IG- Animal 21 days that were immobilized for 7 days ) , Immobilized and Stretched Group (ISG - Animal 21 days that were immobilized for 7 and rehabilitated by stretching for 3 days) and Stretched Group (SG -Animal 21 days not immobilized for 7 days and subsequently stretching for 3 days), Control Group (CG30 - Animals 30 days).Fragments of the soleus muscle was processed under different histochemical methods, hematoxylin - eosin staining and picro-sirius. Variables were evaluated inter - and intra - groups through statistical techniques such as Kruskall Wallis and Dunn\'s post test . Conclusion: The results indicated that the soleus muscle of rats were babies citoarquiteturais significant changes when the manual stretching intermittently been used as a therapeutic resource after 7 days of disuse of the right posterior segment ( immobilization in plantar flexion). The immobilized muscles , both proteins (desmin and vimentin ) content had decreased compared to control values (21 or 30 days), indicating negative after- tissue balance disuse. The amount of these proteins was not modified in animals subjected only to intermittent stretching procedure. The animals underwent immobilization have been rehabilitated and that the amount of desmin was not significantly increased, not reaching values similar to the control group 30 days. These data suggest that desmin filaments need to 3 days longer than the time for rehabilitation to restore the intermittent stretching interstitial fiber architecture and hence favor the mechanical transduction of signals between intra-and extracellular media. However, the effect of stretching on cytoarchitectural intermediate filaments should be followed longitudinally and confirmed in additional biochemical and molecular studies .
Pattabiraman, Sundararaghavan [Verfasser]. "Vimentin protects differentiating stem cells from stress / Sundararaghavan Pattabiraman". Göttingen : Niedersächsische Staats- und Universitätsbibliothek Göttingen, 2020. http://d-nb.info/1223171582/34.
Pełny tekst źródłaMcGinn, Mary Catherine. "Interplay Between Keratin and Vimentin Expression in Oral Cancer". VCU Scholars Compass, 2010. http://scholarscompass.vcu.edu/etd/49.
Pełny tekst źródłaKirmse, Robert. "Studium der Wachstumskinetik von Intermediärfilamenten mit Hilfe von Vimentin". [S.l. : s.n.], 2007. http://nbn-resolving.de/urn:nbn:de:bsz:16-opus-83413.
Pełny tekst źródłaFay, Nikta. "Parvoviral interactions with the cytoskeleton : exposing vimentin – the forgotten player". Thesis, University of British Columbia, 2014. http://hdl.handle.net/2429/50672.
Pełny tekst źródłaScience, Faculty of
Zoology, Department of
Graduate
Rato, Leila Sofia Coelho. "Vimentin interacts with the Akt/mTOR pathway mediating cell growth". Master's thesis, Universidade de Aveiro, 2017. http://hdl.handle.net/10773/22372.
Pełny tekst źródłaA vimentina é uma proteína da classe III dos filamentos intermédios que promove processos tais como proliferação, migração e invasão celular através da interação com diferentes vias de sinalização. No entanto, o papel da vimentina no crescimento celular é ainda pouco conhecido. Neste estudo, observamos que fibroblastos isolados de embriões de ratinhos sem vimentina (Vim -/- MEFs) eram mais pequenos que o tipo normal (WT). Assim, o objetivo deste estudo era entender de que forma a vimentina regula o crescimento celular. Com recurso a modelos in vitro, técnicas de microscopia e técnicas bioquímicas descobrimos que Vim -/- MEFs tinham menor volume e concentração de proteínas quando comparadas com WT MEFs. Adicionalmente, a síntese proteica e ativação de mTORC1 estavam significativamente reduzidas em Vim -/- MEFs. Através de co-imunoprecipitação, descobrimos que a vimentina interage com os complexos mTORC2 e TSC. Assim, postulamos que a vimentina regula o crescimento celular por interação com proteínas da via de sinalização AKT/mTO
Vimentin is a type III intermediate filament protein that takes part in cell proliferation, migration and invasion, by acting as a signalling scaffold. The role of vimentin in cell growth, however, is poorly understood. We observed that vimentin knockout mouse embryonic fibroblasts (Vim -/- MEFs) were smaller than the wild type (WT). Therefore, this work aimed to understanding how vimentin regulates cell growth. Using in vitro models, imaging techniques and biochemical approaches, we have found that the volume and protein concentration of Vim -/- MEFs is lower when compared to WT MEFs. Further, protein synthesis and mammalian target of rapamycin complex 1 (mTORC1) activation was attenuated in Vim -/- MEFs. By co-immunoprecipitation we found that vimentin interacts with mammalian target of rapamycin complex 2 (mTORC2) and tuberous sclerosis protein complex (TSC) after insulin stimulation. Consequently, we postulate that vimentin regulates cell growth by interacting with proteins of the AKT/mTOR pathway
Botelho, Tessa de Lucena. "Expressão imunoistoquímica das proteínas c-erbB-2 e vimentina em carcinomas epidermóides bucais em correlação com características clínicas e prognóstico". Universidade de São Paulo, 2009. http://www.teses.usp.br/teses/disponiveis/23/23141/tde-29092009-081641/.
Pełny tekst źródłaAbout of malignancies in oral mucosa, 95% are represented by oral squamous cell carcinoma (OSCC). This disease is usually aggressive, with unpredictable biological behavior and poor prognosis and the local infiltration and consequent metastases the main cause of death of patients. The identification of molecular markers that may predict the clinical course of disease, guide the treatment as well as lead the development of new therapies that improve the rates of survival, has been the goal of many studies. This study examined the correlation of immunohistochemical expression of c-erbB-2 and vimentin with clinical features and prognosis of OSCC patients from the retrospective analysis of 65 cases. c-erbB-2 and vimentin were expressed in 61.54% and 70.8% of samples, respectively. There was no statistically significant correlation between the expression of these markers and clinical characteristics, but a tendency to vimentin expression in the lymph node status. The patients survival, was influenced by their gender, smoking habits the, clinical stage of disease, N index and modalities of treatment. The gender was the only independent prognostic factor detected. The immunohistochemical expression of c-erbB-2 and vimentin did not show up predictive of survival in oral cavity squamous cell carcinoma.
Block, Johanna Lena [Verfasser]. "Stress-Strain Behavior of Single Vimentin Intermediate Filament / Johanna Lena Block". Göttingen : Universitätsverlag Göttingen, 2018. http://d-nb.info/1170672000/34.
Pełny tekst źródłaMahesh, Balakrishnan. "Role of the anti-vimentin response in rejection of murine cardiac allografts". Thesis, Imperial College London, 2008. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.498312.
Pełny tekst źródłaZehetmayer, Barbara. "Molekulargenetische Analyse der Kandidatengene p97/VCP, Myotilin und Vimentin bei hereditären Myopathien". Diss., Ludwig-Maximilians-Universität München, 2015. http://nbn-resolving.de/urn:nbn:de:bvb:19-180438.
Pełny tekst źródłaDenz, Manuela [Verfasser]. "Influence of Ions on the Assembly of Vimentin Intermediate Filaments / Manuela Denz". Göttingen : Niedersächsische Staats- und Universitätsbibliothek Göttingen, 2020. http://d-nb.info/1227039670/34.
Pełny tekst źródłaKraxner, Julia [Verfasser]. "Vimentin Intermediate Filament Softening - Recovery Behavior and Post-Translational Modifications / Julia Kraxner". Göttingen : Niedersächsische Staats- und Universitätsbibliothek Göttingen, 2021. http://nbn-resolving.de/urn:nbn:de:gbv:7-21.11130/00-1735-0000-0008-590D-7-0.
Pełny tekst źródłaBen, Azzouz Eya. "Étude physiopathologique des infections à Tropheryma whipplei". Thesis, Aix-Marseille, 2019. http://www.theses.fr/2019AIXM0243.
Pełny tekst źródłaWhipple disease is a rare systemic disease caused by the bacterial agent Tropheryma whipplei. T. whipplei presents a particular tropism for macrophages. It induces an atypical macrophage activation program, characterized by the expression of anti-inflammatory molecules, the secretion of IL-16 and the induction of apoptosis. During this thesis, we analyzed the interaction between T. whipplei and myeloid cells (macrophages and monocytes). First, we focused on an immunomodulatory molecule, HLA-G which has previously been associated with immunotolerance mechanisms. We showed that HLA-G is strongly expressed and secreted in vivo in patients with classical Whipple disease. In addition, we found that in vitro, infection of monocytes by T.whipplei induced HLA-G expression accompanied with low TNF secretion. On the other hand, we have shown that a neutralizing antibody against HLA-G increased TNF secretion by monocytes in response to T whipplei, while a TNF inhibitor promoted bacterial replication. Thus, we were able to highlight the involvement of HLA-G in the persistence of T. whipplei within immune cells, then providing a proper environment for its replication.Second, we looked at the early events of T. whipplei interaction with immune cells in order to identify a cell partner who could interact with T. whipplei. We showed that vimentin, a cytoskeletal protein belonging to the intermediate filaments, is specifically involved in the adhesion and internalization of T. whipplei. In conclusion, this work has provided some answers to a better understanding of T. whipplei infections
Paulin-Levasseur, Micheline. "Cellular dynamics of vimentin filaments and their spatial relationship to microtubules in lymphocytes". Thesis, University of Ottawa (Canada), 1987. http://hdl.handle.net/10393/5396.
Pełny tekst źródłaTezcan, Okan. "Metastatic Behaviour Of Doxorubicin Resistant Mcf-7 Breast Cancer Cells After Vimentin Silencing". Master's thesis, METU, 2013. http://etd.lib.metu.edu.tr/upload/12615553/index.pdf.
Pełny tekst źródłaBird, Danielle N. "Regulatory Analysis of Vimentin Expression in Metastatic Versus Nonmetastatic Breast Cancer Cell Lines". VCU Scholars Compass, 1994. http://scholarscompass.vcu.edu/etd/4366.
Pełny tekst źródłaLeong, Hon Sing. "The PAI-1-vitronectin-vimentin ternary complex : mechanism of extracellular assembly and role in transplant vasculopathy". Thesis, University of British Columbia, 2008. http://hdl.handle.net/2429/2509.
Pełny tekst źródłaKim, Ryung Rae. "Investigation of the action of new pentapeptides entities and the interaction between human group IIA phospholipase A2 and vimentin". Thesis, The University of Sydney, 2018. http://hdl.handle.net/2123/19853.
Pełny tekst źródłaLazaruk, O. V. "Vimentin expression of parenchymal cells and stromal cells of ductal breast carcinoma: comparative characteristics". Thesis, Sumy State University, 2016. http://essuir.sumdu.edu.ua/handle/123456789/44936.
Pełny tekst źródłaPaccione, Rachel J. "Vimentin Overexpression Contributes To the Biological Properties of Metastatic Head and Neck Cancer Cells". VCU Scholars Compass, 2005. http://scholarscompass.vcu.edu/etd/1084.
Pełny tekst źródłaLardier, Nathan. "Mechanics of intermediate filaments and microtubules in living cells". Electronic Thesis or Diss., Université Paris sciences et lettres, 2021. http://www.theses.fr/2021UPSLS058.
Pełny tekst źródłaAlthough extensively studied in vitro, the mechanics of the cytoskeleton is still largely unexplored in living cells. We use an intracellular optical tweezers-based micromanipulation technique to apply forces directly on cytoskeletal filaments in order to probe microtubules and intermediate filament mechanics and focus on how they interact mechanically. Measuring simultaneously the force applied to the filaments and their deflection, i.e. the deformation of the filaments perpendicular to their axis, as a function of time, allows us to deduce the force-deflection curves of the filaments and to characterize the rigidity of vimentin intermediate filaments and microtubules. By fitting the force-deflection curves at small forces, we show that microtubules have a lower effective stiffness than vimentin upon deflection. We then apply forces twice on the same cytoskeletal bundle to show that vimentin filaments, but not microtubules, stiffen more than three times upon repeated deflections. We further characterize the mechanical coupling between vimentin filaments and microtubules by using microtubule destabilizing and stabilizing drugs and by increasing microtubule acetylation. Interestingly, we find that these modifications do not affect the effective stiffness of vimentin filaments while destabilizing or acetylating microtubules significantly reduces vimentin filament stiffening upon repeated deflection. Altogether, these results suggest that microtubules promote stiffening of vimentin bundles under repeated mechanical stress. In sharp contrast, in cells knockout for vimentin, the mechanical properties of microtubules are unchanged. Our findings highlight the importance of the interactions between microtubules and intermediate filaments in cell mechanics and suggest that vimentin intermediate filaments are mechanosensitive structures which exhibit history-dependent mechanoresponses
Espejel, del Moral María del Carmen. "Histomorphologische und immunhistologische Charakterisierung der Endometrose beim Rind". Doctoral thesis, Universitätsbibliothek Leipzig, 2012. http://nbn-resolving.de/urn:nbn:de:bsz:15-qucosa-99530.
Pełny tekst źródłaThiagarajan, Praveena S. "VIMENTIN IS A PHOSPHORYLATED TARGET OF MCP-1-INDUCED PKCβ ACTIVATION AND AN ENDOGENOUS LIGAND FOR THE INNATE IMMUNE RECEPTOR DECTIN-1". Kent State University / OhioLINK, 2010. http://rave.ohiolink.edu/etdc/view?acc_num=kent1399566096.
Pełny tekst źródła井川, 敬介. "Polo-like kinase1はvimentinのリン酸化を介して分裂期において初期エンドソームの膜融合を阻害する". 京都大学 (Kyoto University), 2014. http://hdl.handle.net/2433/188844.
Pełny tekst źródłaWu, Wei. "Unveiling the neglected roles of nucleoprotein NLS2 and cellular vimentin during Influenza A virus infection". Thesis, University of British Columbia, 2014. http://hdl.handle.net/2429/51474.
Pełny tekst źródłaScience, Faculty of
Zoology, Department of
Graduate
Gumprecht, Annett. "Analyse morphometrischer Messungen an Astrozyten des Hippokampus von Wildtypmäusen im Vergleich zu GFAP-/- - Vimentin-/- - Mäusen". Doctoral thesis, Universitätsbibliothek Leipzig, 2013. http://nbn-resolving.de/urn:nbn:de:bsz:15-qucosa-129946.
Pełny tekst źródłaKnecht, Sharmon M. "PI3K mediates S. aureus invasion leading to peri-nuclear vimentin collapse in human endothelial cells". Virtual Press, 2005. http://liblink.bsu.edu/uhtbin/catkey/1319545.
Pełny tekst źródłaDepartment of Biology
Wiseman, J. "Relocalisation of β-globin coding sequences by myosin heavy chain and vimentin 3' untranslated regions". Thesis, University of Aberdeen, 1996. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.318982.
Pełny tekst źródłaMostajeran, Zahra [Verfasser]. "The influence of vimentin on actin dynamics and force generation in RPE1 cells / Zahra Mostajeran". Saarbrücken : Saarländische Universitäts- und Landesbibliothek, 2021. http://d-nb.info/1237268761/34.
Pełny tekst źródłaJungert, Kerstin. "Untersuchungen zur Bedeutung des Transkriptionsfaktors Sp1 in der TGF-beta-1-vermittelten Progression des Pankreaskarzinoms". [S.l. : s.n.], 2006. http://nbn-resolving.de/urn:nbn:de:bsz:289-vts-55615.
Pełny tekst źródłaNair, K. Sukumaran. "The role of anti-vimentin antibodies in graft dysfunction after cardiac transplantation in a mouse model". Thesis, Imperial College London, 2006. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.436124.
Pełny tekst źródłaDune, Ana Cláudia [UNESP]. "Expressão de recptor de estrógeno, vimentina, TGFbeta, e marcador de macrófagos em tumor ósseo de células gigantes em gatos domésticos". Universidade Estadual Paulista (UNESP), 2008. http://hdl.handle.net/11449/95976.
Pełny tekst źródłaFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
O tumor ósseo de células gigantes apresenta 3 diferentes tipos celulares, sendo duas estromais: fibroblastos neoplásicos e células mononucleares; e o terceiro tipo, células gigantes. Propôs-se que este tumor é de linhagem monócito-macrofágica e acredita-se que as células gigantes se formam por fusão de células mononucleares. Aparentemente os fibroblastos neoplásicos que expressam o fator transformador de crescimento TGFbeta1 estão envolvidos no recrutamento das células gigantes para o tumor. Com o intuito de compreender melhor a histogênese, o envolvimento do estrógeno e a expressão de receptores TGFbeta1, foi realizado este estudo em casos deste tumor em gatos domésticos. Para tanto foi utilizado o método imuno-enzimático Streptoavidina-biotina utilizando-se o anticorpo primário anti-vimentina, clone 3B4 (Dako A/s, Denmark); o anticorpo marcador de macrófago, MAC387 (Dako A/s, Denmark); o anticorpo para receptores de estrógeno, clone 15D (Dako A/s, Denmark) e o anticorpo marcador para TGFBeta1 (Santa Cruz Biotechnology). Os resultados foram analisados pela porcentagem e desvio padrão de células marcadas para cada anticorpo e permitiram concluir que: o TOCG de gatos domésticos, assim como em humanos, tem origem mesenquimal e expressa receptores de estrógeno e de TGF 1 e as células gigantes do tumor não reagem com o clone 387, marcador de células de linhagem mielomonocítica.
Giant cell tumor of bone are composed of 3 different cell types: round mononuclear stromal cells, spindle-shaped mononuclear stromal cells, and giant cells. Some authors assert giant cell could arise by fusion of mononuclear cell mielomonocytic. Aparently neoplasic fibroblast that expression TGF is involved in the recruitment of giant cells from tumor. For better understand of histogenesis, the involved of receptors estrogen and of expression of TGF receptors, achieved this study in this cases tumor in domestics cats. By using the immune-enzymatic Streptoavidin- biotin using the primary antibody anti-vimentin, clone VIM 3B4 (Dako A/s, Denmark); antibody myeloid/histocyte clone MAC 387 (Dako A/s, Denmark); antibody estrogen receptor clone 1D5 (Dako A/s, Denmark) and the antibody TGFb1 (Santa Cruz Biotechnology). The results analyzed for percent and standara deviation of marks cells for each antibody and permissive to come to a conclusion: the GCT of bone in domestic cats, like humans, has mesenchymal origin and has expression of estrogen receptors and of TGFbeta, and giants cells this tumor not react with the clone 387, mark the cells myeloidmonocyte lineage.
TUVERI, ROSSANA. "Identification & characterization of natural and synthetic compounds as new anticancer agents". Doctoral thesis, Università degli Studi di Cagliari, 2016. http://hdl.handle.net/11584/266770.
Pełny tekst źródłaZehetmayer, Barbara [Verfasser], i Sabine [Akademischer Betreuer] Krause. "Molekulargenetische Analyse der Kandidatengene p97/VCP, Myotilin und Vimentin bei hereditären Myopathien / Barbara Zehetmayer. Betreuer: Sabine Krause". München : Universitätsbibliothek der Ludwig-Maximilians-Universität, 2015. http://d-nb.info/1069743380/34.
Pełny tekst źródłaCarse, Sinead. "Characterisation of human surfactant protein A and recombinant human vimentin in their modulation of HPV16 pseudovirus infection". Master's thesis, Faculty of Health Sciences, 2019. https://hdl.handle.net/11427/31719.
Pełny tekst źródłaIshigooka, Nozomi. "Predicting factors for disappearance of anti-mutated citrullinated vimentin antibodies in sera of patients with rheumatoid arthritis". Doctoral thesis, Kyoto University, 2020. http://hdl.handle.net/2433/245829.
Pełny tekst źródła0048
新制・課程博士
博士(医学)
甲第22144号
医博第4535号
新制||医||1039(附属図書館)
京都大学大学院医学研究科医学専攻
(主査)教授 松田 秀一, 教授 生田 宏一, 教授 杉田 昌彦
学位規則第4条第1項該当
Doctor of Medical Science
Kyoto University
DFAM
Schorling, Jamie J. "Biochemical and Immunocytochemical Characterization of Canine Corneal Cells Cultured in Two Different Media". Thesis, Virginia Tech, 2007. http://hdl.handle.net/10919/32443.
Pełny tekst źródłaMaster of Science
Colakoglu, Gulsen. "Assembly Dynamics of Intermediate Filaments". The Ohio State University, 2009. http://rave.ohiolink.edu/etdc/view?acc_num=osu1247691189.
Pełny tekst źródłaPritchard, Adaleigh Elizabeth. "Modeling End-to-End Annealing of Intermediate Filaments". The Ohio State University, 2014. http://rave.ohiolink.edu/etdc/view?acc_num=osu1397743583.
Pełny tekst źródłaPaula, Fernanda Oliveira de. "Avaliação da imunoexpressão de vimentina e de osteopontina no reparo ósseo precoce de defeitos preenchidos com enxerto bovino associado à terapia laser de baixa intensidade". Universidade Federal de Juiz de Fora (UFJF), 2010. https://repositorio.ufjf.br/jspui/handle/ufjf/2638.
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O objetivo deste estudo foi avaliar, pelo método de imunoistoquímica, a expressão de vimentina e osteopontina durante as fases iniciais de reparo de defeitos ósseos criados em fêmures de ratos Wistar albinus tratados com enxerto ósseo bovino orgânico Gen-ox® em associação com terapia laser de baixa intensidade. Foram selecionadas de forma aleatória 24 amostras emblocadas de arquivo provenientes de trabalho experimental desenvolvido anteriormente, no qual foram efetuadas análises histológicas e histomorfométricas de fêmures de cinquenta ratos. Obtiveram-se lâminas histológicas dos blocos de diferentes animais, os quais estavam previamente divididos, de acordo com o tratamento realizado, da seguinte forma: grupo I (controle), grupo II (Gen-Ox®) e grupo III (LLLT e Gen-Ox®). Foram elaboradas quatro lâminas para cada um dos tempos experimentais de 3, 5, 7 e 15 dias para cada grupo. Duas lâminas foram utilizadas para analisar a expressão da osteopontina e duas para vimentina, totalizando 48 lâminas. Em cada lâmina considerou-se dois campos para análise, sendo um campo na região da interface osso-defeito e o outro próximo ao periósteo, em um total de 96 campos. Para realização da reação imunoistoquímica anti-vimentina e anti-osteopontina utilizou-se o método clássico do complexo avidina-biotina peroxidase anti-peroxidase. A marcação positiva foi determinada pela identificação de coloração castanha intracitoplasmática nas reações com ambos os anticorpos. Os cortes foram analisados em microscópio Zeiss em aumentos de 200x, 400x e 1000x, em toda extensão, por dois diferentes observadores. Determinou-se, por método de contagem semiquantitativo, a média de intensidade de células positivas marcadas nos campos dos tratamentos propostos em cada período, o qual foi classificado pelo sistema de escore de acordo com os seguintes parâmetros: 0 = ausência de marcação; + = marcação leve (até 1/3 de células positivas); ++ = marcação moderada (até 2/3 de células positivas); e +++ = marcação intensa (acima de 2/3 de células marcadas). Os resultados mostraram que todos os grupos apresentaram marcação para vimentina e para osteopontina em todos os períodos do experimento. Observou-se marcação celular mais acentuada para vimentina no período cicatricial inicial no grupo III. Não foram verificadas diferenças na marcação para osteopontina nos animais submetidos à terapia laser de baixa intensidade associada ao enxerto quando comparado aos outros grupos.
The aim of this study was to evaluate, by immunohistochemistry, the expression of vimentin and osteopontin during the early stages of repair of bone defects created in femurs of Wistar albinus rats treated with organic bovine bone graft Gen-ox® and associated with low level laser therapy. Twenty four embedded samples were randomly selected from the file of a previous experimental work, in which histological analysis and histomorphometry of the femurs of fifty rats was performed. Histological slides were obtained from blocks of different animals which were divided in accordance to previous treatment as follow: group I (control), group II (Gen-Ox ® ) and group III (LLLT and Gen-Ox ®). Four slides were prepared for each of the experimental time of 3, 5, 7 and 15 days for each group. Of the four slides, two were assessed for the expression of osteopontin and two of vimentin, in a total of 48 slides. On each slide two fields were considered for analysis: one in the bone-defect interface and the other near the periosteum, in a total of 96 fields. To perform the immunohistochemistry anti-vimentin and anti-osteopontin, we used the classical avidin-biotin peroxidase anti-peroxidase method. The positive staining was determined by identification of intracytoplasmic brown color in the reactions with both antibodies. The sections were analyzed in Zeiss microscope at a magnification of 200x, 400x and 1000x by two different observers. The average intensity of positive cells stained in the fields in each period was determined by a semiquantitative counting method which was classified by a scoring system as follow: 0 = no marking; + = mild labeling (up to one third of positive cells); + + = moderate labeling (up to two thirds of positive cells) and + + + = intense labeling (over two thirds of labeled cells). The results showed that all groups had marked for vimentin and osteopontin in all periods of the experiment. We observed a stronger cell labeling for vimentin in the initial healing period in group III. There were no differences in cell labeling for osteopontin in animals subjected to low level laser therapy associated with graft as compared to other groups.
Block, Johanna Lena [Verfasser], Sarah [Akademischer Betreuer] Köster, Sarah [Gutachter] Köster i Andreas [Gutachter] Janshoff. "Stress-Strain Behavior of Single Vimentin Intermediate Filaments / Johanna Lena Block ; Gutachter: Sarah, Köster; Andreas Janshoff ; Betreuer: Sarah, Köster". Göttingen : Niedersächsische Staats- und Universitätsbibliothek Göttingen, 2018. http://d-nb.info/1164231197/34.
Pełny tekst źródłaRapatoni, Liane. "Expressão de CK5 e vimentina/E-caderina nos diferentes subtipos de carcinomas ductais mamários". Universidade de São Paulo, 2013. http://www.teses.usp.br/teses/disponiveis/17/17145/tde-27102013-000552/.
Pełny tekst źródłaIntroduction: Molecular markers have been used to identify subgroups of tumors with distinct clinical behavior, including recurrence pattern. Objective: To characterize the immunohistochemical expression of vimentin (VIM) and E-cadherin (CDH1) in invasive ductal carcinoma (IDC) of the breast and its association with cytokeratin 5 (CK5) expression and clinicopathological features. Methods: A tissue microarray was constructed from 82 IDC breast cancer specimens. Immunohistochemistry (IHC) was used to determine hormone receptor (HR; estrogen and progesterone receptors) status and human epidermal growth factor receptor 2 (HER2), VIM, CDH1, CK5, and Ki-67 expression. Tumors were classified as luminal A (HR+, HER2-), luminal B (HR+, HER2+ or high Ki-67), HER2 enriched (HR-, HER2+), and triple negative (TNBC; HR-, HER2-). Results: The VIM+/CDH1-/low phenotype was not observed in luminal A, luminal B and HER2 enriched tumors, whereas this phenotype was present in 61.9% of triple negative (TNBC) tumors (p = 0.0001). The median Ki-67 index in VIM+/CDH1-/low tumors was 13.6 (range, 17.8-45.4) compared with 9.8 (range, 4.1-38.1) in non-VIM+/CDH1-/low tumors (p= 0.0007). The presence of lymph node metastasis was less frequent in patients with VIM+/CDH1-/low tumors than in those with non-VIM+/CDH1-/low tumors (23% vs. 61%; 2 test, p= 0.01). The 5-years disease-free survival was 61.5% and 83.7% (log-rank test; p= 0.02) and the 5-year overall survival was 51.2% and 83.5% (log-rank test; p= 0.03) in patients with VIM+/CDH1-/low phenotype and non-VIM+/CDH1-/low phenotype tumors, respectively. Conclusion: The expression of VIM and CDH1 identified a subset of IDC breast cancer of the mesenchymal phenotype with high histological grade and high mitotic index.
Suasnavas, Edison A. "Characterization and Potential Utility of Porcine Trophoblast-Derived Stem-Like Cells". DigitalCommons@USU, 2013. http://digitalcommons.usu.edu/etd/1540.
Pełny tekst źródłaBrennich, Martha. "Cation induced self-assembly of intermediate filaments". Doctoral thesis, Niedersächsische Staats- und Universitätsbibliothek Göttingen, 2012. http://hdl.handle.net/11858/00-1735-0000-001A-6BD9-E.
Pełny tekst źródłaTuleneva, O. А., i I. S. Davydenko. "Immunohistochemical vimentin concentration in the endothelium of the terminal chorionic villi in the aspect of various forms of placental insufficiency". Thesis, БДМУ, 2017. http://dspace.bsmu.edu.ua:8080/xmlui/handle/123456789/16789.
Pełny tekst źródłaBowers, Hannah Elizabeth, i Jennifer Hall. "THE EFFECTS OF ESTROGEN-INDUCED STROMAL CELL EFFECTORS, OSTEOPONTIN AND VIMENTIN, ON CHLAMYDIA INFECTIONS IN A NON-POLARIZED CELL CULTURE MODEL". Digital Commons @ East Tennessee State University, 2018. https://dc.etsu.edu/asrf/2018/schedule/98.
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