Gotowa bibliografia na temat „TRNAfMet”

Formylation of the initiator methionyl-tRNA (Met-tRNAfMet) was generally thought to be essential for initiation of protein synthesis in all eubacteria based on studies conducted primarily inEscherichia coli. However, this view of eubacterial protein initiation has changed because some bacteria have been demonstrated to have the capacity to initiate protein synthesis with the unformylated Met-tRNAfMet. Here we show that thePseudomonas aeruginosainitiation factor IF-2 is required for formylation-independent protein initiation inP. aeruginosa, the first bacterium shown to have the ability to initiate protein synthesis with both the initiator formyl-methionyl-tRNA (fMet-tRNAfMet) and Met-tRNAfMet. TheE. coliIF-2, which participates exclusively in formylation-dependent protein initiation inE. coli, was unable to facilitate utilization of Met-tRNAfMetin initiation inP. aeruginosa. However, theE. coliIF-2 was made to function in formylation-independent protein initiation inP. aeruginosaby decreasing the positive charge potential of the cleft that binds the amino end of the amino acid attached to the tRNA. Furthermore increasing the positive charge potential of this cleft in theP. aeruginosaIF-2 prevented the protein from participating in formylation-independent protein initiation. Thus, this is the first demonstration of a eubacterial IF-2 with an inherent capacity to facilitate utilization of Met-tRNAfMetin protein initiation, discounting the dogma that eubacterial IF-2 can only allow the use of fMet-tRNAfMetin protein initiation. Furthermore these findings give important clues to the basis for discriminating the initiator Met-tRNA by IF-2 and for the evolution of alternative mechanisms for discrimination.

ABSTRACT Protein synthesis in eukaryotic organelles such as mitochondria and chloroplasts is widely believed to require a formylated initiator methionyl tRNA (fMet-tRNAfMet) for initiation. Here we show that initiation of protein synthesis in yeast mitochondria can occur without formylation of the initiator methionyl-tRNA (Met-tRNAfMet). The formylation reaction is catalyzed by methionyl-tRNA formyltransferase (MTF) located in mitochondria and usesN 10-formyltetrahydrofolate (10-formyl-THF) as the formyl donor. We have studied yeast mutants carrying chromosomal disruptions of the genes encoding the mitochondrial C1-tetrahydrofolate (C1-THF) synthase (MIS1), necessary for synthesis of 10-formyl-THF, and the methionyl-tRNA formyltransferase (open reading frame YBL013W; designated FMT1). A direct analysis of mitochondrial tRNAs using gel electrophoresis systems that can separate fMet-tRNAfMet, Met-tRNAfMet, and tRNAfMet shows that there is no formylation in vivo of the mitochondrial initiator Met-tRNA in these strains. In contrast, the initiator Met-tRNA is formylated in the respective “wild-type” parental strains. In spite of the absence of fMet-tRNAfMet, the mutant strains exhibited normal mitochondrial protein synthesis and function, as evidenced by normal growth on nonfermentable carbon sources in rich media and normal frequencies of generation ofpetite colonies. The only growth phenotype observed was a longer lag time during growth on nonfermentable carbon sources in minimal media for the mis1 deletion strain but not for thefmt1 deletion strain.

Translational initiation factor 2 (IF2) is the largest of the 3 factors required for translation initiation in prokaryotes and has been shown to be essential in Escherichia coli. It stimulates the binding of fMet-tRNAfMet to the 30S ribosomal subunit in the presence of GTP. The selectivity is achieved through specific recognition of the tRNAfMet blocked α-amino group. IF2 is composed of 3 structural domains: N-domain, whose function is not known; G-domain, which contains the GTP/GDP binding site and the GTPase catalytic center; and C-domain, which recognizes and binds fMet-tRNAfMet. Its activity is strictly bacteria specific and highly conserved among prokaryotes. So far, antibiotics targeting IF2 function are not known, and this makes it an ideal target for new drugs with mechanisms of resistance not yet developed. A few assays have been developed in the past, which allow the detection of IF2 activity either directly or indirectly. In both instances, the assays are based on radioactive detection and do not allow for high throughput because of the need for separation or solvent extraction steps. The authors describe a novel biochemical assay for IF2 that exploits the molecular recognition of fMet-tRNAfMet by the C-domain. The assay is based on the incubation of biotinyl-IF2 with fMet-tRNAfMet and the subsequent capture of the radiolabeled complex by streptavidin-coated beads, exploiting the scintillation proximity assay (SPA) technology. The assay has been designed in an automatable, homogeneous, miniaturized fashion suitable for high-throughput screening and is rapid, sensitive, and robust to dimethyl sulfoxide (DMSO) up to 10% v/v. The assay, used to screen a limited chemical collection of about 5000 compounds and a subset of compounds originated by a 2-D substructural search, has shown to be able to detect potential IF2 inhibitors.

Aim of this work was to identify nucleotide sequences of tRNAMet of S. globisporus 1912-2. Methods. Resources of server NCBI (programs BLAST: blast, discontiguous megablast and databases: “Genome”, “Nucleotide”) were used for in silico analysis of library of S. grlobisporus 1912-2 contigs. Results. Nucleotide sequences of 4 genes of tRNAMet of S. globisporus 1912-2 were determined in silico. Molecules of tRNA of the II type were translated from tRNAMet gene (Contig No 21 (936–1008 bp)) and the molecules of tRNAfMet genes (Contigs No 299 (1713–1787 bp), No 255 (5941–6015 bp)). Conclusions. 4 genes of transfer RNAs-methionine were identified in silico in S. globisporus 1912-2 genome. Two genes from them coded tRNAfMet molecules. The nucleotide sequences of all tRNAMet genes were identified.Keywords: gene, tRNA, methionine, nucleotide sequence, Streptomyces.

ABSTRACT Bacillus subtilis mutants with resistance against peptide deformylase inhibitors were isolated. All showed a bypass of the pathway through mutations in three genes required for formylation of Met-tRNAfMet, fmt, folD, and glyA. glyA corresponds to a yet uncharacterized locus inducing resistance. The bypass of formylation caused robust fitness reduction but was not accompanied by alterations of the transcription profile. A subtle adaptation of the enzymes of the intermediary metabolism was observed.

Thesis (Ph. D.)--University of Texas at Austin, 2001.
"Fmet" after tRNA in title is superscript. Vita. Includes bibliographical references (leaves 102-119). Available also in a digital version from Dissertation Abstracts.

Protein synthesis in all organisms utilizes a special tRNA called the initiator tRNA. Initiator tRNAs take part in the initiation step of protein synthesis by their direct binding to the P‐site of the ribosome. The other tRNAs (elongator tRNAs) bind first to the A‐site of the ribosome and are subsequently translocated to the P‐site during elongation. The initiator tRNA possesses sequence and structural characteristics, which enable it to perform its unique function in protein synthesis. In addition to the highly conserved three consecutive G:C base pairs in the anticodon stem of the initiator tRNA which facilitate its P‐site binding, bacterial and organellar initiator tRNAs are also formylated by FMT (methionyl‐tRNAfMet formyltransferase) to enable their binding to initiation factor 2 (IF2), directing them specifically into initiation. Structure‐function studies of E. coli initiator tRNA in‐vivo using reporter constructs showed that formylation plays a crucial role in deciding the fate of the initiator tRNA in initiation. The tRNA mutants deficient in formylation take part in initiation and/or elongation. Protein factors like IF2, elongation factor Tu (EF‐Tu) and peptidyl‐tRNA hydrolase (Pth) also contribute to the fate of the tRNA in‐vivo. The current study aims to understand how the balance of protein factors and sequence elements present on a tRNA determine its participation at the steps of initiation and/or elongation using E. coli and M. smegmatis as model organisms. The findings of my research have been described in three distinct investigations as follows: PART‐I. Development of assay systems for amber codon decoding at the steps of initiation and elongation by tRNAfMet derivatives in mycobacteria The bulk of our understanding of the mechanism of protein synthesis in bacteria is derived from the studies in E. coli. The mechanism of translation in Gram positive bacteria remains a relatively less understood process. Gram positive bacteria possess significant differences in their translational apparatus as compared to the Gram negative organisms, and therefore present with interesting systems to understand the mechanism of translation. For example, Gram positive bacteria use an indirect pathway for synthesis of Gln‐tRNAGln and Asn‐tRNAAsn as opposed to direct synthesis of Gln‐tRNAGln by glutaminyl‐tRNA synthetase (GlnRS) in E. coli. We used M. smegmatis, a slow growing Gram positive bacterium, as the model organism to study translation. The understanding of protein synthesis in these bacteria has been limited by the lack of well characterized genetic systems. Using chloramphenicol acetyltransferase (CAT) reporters (having an amber codon as the start codon or as a codon at an internal position within the reading frame of the mRNA), we developed genetic systems where the amber codon is decoded by a mutant initiator tRNA (wherein the CAU anticodon was mutated to CUA with or without additional changes in the acceptor stem) either at the step of initiation or elongation in M. smegmatis, enabling us to measure the efficiency of the mutant tRNAs in initiation or elongation in‐vivo. Characterization of the reporter encoded protein by mass spectrometric analysis showed that initiation in such a reporter proceeds through incorporation of methionine, as opposed to the use of glutamine in similar systems in E. coli. Elongation in the reporter system, carried out by formylation deficient mutants of the initiator tRNA, was found to occur through the insertion of glutamine using a two‐step pathway, where the tRNA is first aminoacylated by a non‐discriminating GluRS, followed by transamidation of the attached Glu by an amidotransferase. The formylation deficient acceptor stem mutants of the initiator tRNA were also recognized differentially by the amidotransferase in‐vivo, leading to the insertion of either glutamate or glutamine during elongation by different mutants of the tRNA. Overall, the study highlighted the conserved nature of formylation across bacteria, and its importance in the exclusive participation of the initiator tRNA in initiation. PART II. Physiological role of tRNAs that function as alternate initiator tRNAs in mycobacteria The genomes of M. tuberculosis and M. smegmatis encode three tRNAs, metU, metV and metT, with CAU anticodons. While metU has been shown to encode the initiator tRNA in these bacteria, the other two (metV and metT) are both annotated as methionine elongator tRNA. Interestingly, these tRNAs also possess some sequence characteristics like the G:C base pairs in the anticodon stem, and the lack of a Watson‐Crick base pair at 1:72 position, which are thought to be restricted to initiator tRNAs. We were interested in understanding the physiological role of such sequence elements present in the elongator tRNA. Computational and biochemical characterization of metT and metV identified them as the methionine decoding elongator tRNA (tRNAMet) and the minor form of the isoleucine decoding tRNA (tRNAIle2), respectively. Mass spectrometric analysis of metV showed that the C34 in the anticodon of the tRNA is modified to lysidine, which is consistent with its role in AUA decoding. Interestingly, the expression of metV is upregulated under stress conditions. Analysis of the modification status of the tRNA under hypoxic conditions showed that the tRNA is undermodified for lysidine, which would preclude its function in AUA decoding and enable it to function as tRNAMet. We propose that this could be a mechanism of regulation employed by cells under hypoxia. In‐vivo assays using the CAT reporter showed that metV is capable of initiating protein synthesis, providing support to our hypothesis that such tRNAs could function as alternate initiator tRNAs under stress conditions. PART III. Mitochondria‐like sustenance of E. coli on a single tRNAfMet Most organisms possess distinct methionine tRNAs that participate at the steps of initiation and elongation. However, protein synthesis in mammalian mitochondria utilizes only a single tRNAMet, which functions in both initiation and elongation. The partitioning of tRNA into initiation and elongation phases is thought to occur due to the competition between EF‐Tumt (which directs the tRNA to the elongation step) and Fmtmt (which, following formylation, directs the tRNA to the initiation step), for their binding to the single species of Met‐tRNAMet. Our studies on initiator tRNA from mycobacteria, along with the available literature on mutants of E. coli initiator tRNA, show that some mutants of initiator tRNA are capable of participating at the step of elongation. Similarly, as shown in Part‐II of the results section, native elongator tRNAs capable of initiating protein synthesis also exist in some organisms. We therefore asked if in E. coli, a single tRNAMet could participate at both the steps of initiation and elongation (as in mitochondria), and if such tRNAs fulfill the cellular needs at both steps. Using a transduction based genetic strategy, we have shown that many acceptor stem mutants of the initiator tRNA sustain E. coli for initiation and elongation function separately. Importantly, a subset of these mutants also sustains the growth of E. coli devoid of all the six copies of methionine tRNA genes (four of initiators and two of elongators). Furthermore, the mutant tRNA which is most efficient at sustaining the cell is also the one which is most like mitochondrial tRNAMet. Overall, the study provides insights into how distribution of the tRNA between FMT/IF2 and EF‐Tu determines its role in protein synthesis.