Artykuły w czasopismach: „Tissue engineering – laboratory manuals”

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Karthikeyan Ramalingam,. "Innovations in Oral Pathology Laboratory - A Mini Review". International Journal of Head and Neck Pathology 6, nr 2 (13.10.2023): 1–5. http://dx.doi.org/10.56501/intjheadneckpathol.v6i1.914.

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Over the past two decades, a multitude of technological advancements have been integrated into histopathology laboratories, offering tools to enhance standardization and ensure occupational safety. Digital tracking plays a central role in guiding the entire process, from labeling cassettes and slides to the final stages of generating whole slide images, and storage of tissue blocks and tissue sections. Versatile equipment has effectively replaced time-consuming manual tasks, which were susceptible to errors and material loss. Currently, collaborative robots are assuming responsibilities once exclusively carried out by humans. The emergence of these novel technologies is anticipated to help in improving oral pathology laboratory practices.

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Iglesias, Carla, Lu Luo, Javier Martínez, Daniel J. Kelly, Javier Taboada i Ignacio Pérez. "Obtaining the sGAG distribution profile in articular cartilage color images". Biomedical Engineering / Biomedizinische Technik 64, nr 5 (25.09.2019): 591–600. http://dx.doi.org/10.1515/bmt-2018-0055.

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Abstract The articular cartilage tissue is an essential component of joints as it reduces the friction between the two bones. Its load-bearing properties depend mostly on proteoglycan distribution, which can be analyzed through the study of the presence of sulfated glycosaminoglycan (sGAG). Currently, sGAG distribution in articular cartilage is not completely known; it is calculated by means of laboratory tests that imply the inherent inaccuracy of a manual procedure. This paper presents an easy-to-use desktop software application for obtaining the sGAG distribution profile in tissue. This app uses color images of stained cartilage tissues taken under a microscope, so researchers at the Trinity Centre for Bioengineering (Dublin, Ireland) can understand the qualitative distribution of sGAG with depth in the studied tissues.

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Newman-Gage, Helen. "Application of Quality Assurance Practices in Processing Cells and Tissues for Transplantation". Cell Transplantation 4, nr 5 (wrzesień 1995): 447–54. http://dx.doi.org/10.1177/096368979500400506.

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Attention to issues of quality assurance from the early stages of development of experimental cell therapies provides a margin of safety for recipients. Adherence to minimum standards of practice at acquisition, processing, storage, and implantation ensures not only this baseline safety factor for patients, but also provides a baseline for comparative evaluations between different studies or different banks. This paper describes the basic components of a quality assurance program tailored to laboratories and facilities that collect, process, or distribute human cells and tissues for transplantation. These components include policy and procedure manuals, donor screening practices, processing procedures evaluation and control, training and education programs, auditing and investigation roles, responsibility for release of grafts, and recordkeeping and traceability requirements. References and resources for detailed information related to good manufacturing practices and good clinical and laboratory practices are provided. Standards, regulations, and current legislation specifically related to human cells and tissues intended for transplantation are also discussed.

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Speicher, Timothy E., Noelle M. Selkow i Aric J. Warren. "Manual Therapy Improves Immediate Blood Flow and Tissue Fiber Alignment of the Forearm Extensors". Journal of Physical Medicine and Rehabilitation 4, nr 2 (24.08.2022): 28–36. http://dx.doi.org/10.33696/rehabilitation.4.029.

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Background: Manual therapy is commonly used by clinicians to improve blood flow and tissue fiber orientation. Hypothesis/Purpose: Using diagnostic ultrasound, the purpose of the study was to examine how the application of Positional Release Therapy (PRT), instrumented assisted soft tissue mobilization (IASTM), therapeutic ultrasound (US) and a combination of all three, affect lateral elbow immediate blood flow and tissue fiber alignment. Study Design: Controlled laboratory study. Methods: Twenty-five participants (26.0 ± 4.5 years; 69.3 ± 4.3 cm; 81.8 ± 16.9 kg) received PRT =13, US =12, IASTM =13, and a combination treatment = 12. Results: Blood flow was significantly higher following PRT (691.54 ± 1237.16 mm2) compared to IASTM (18.73 ± 227.10 mm2) (p=0.050; ES=0.073 (0.16-1.5) and US (-10.09 ± 479.26 mm2) (p=0.042; ES=0.72 (-0.03-1.29), but no different from the combination intervention (627.64 ± 820.22 mm2) (p=0.849). Seventy-five percent of elbows in the PRT intervention showed improvement in blood flow, 54% in the IASTM group, 45% in US, and 73% in the combination group. Tissue fiber alignment was significantly better following IASTM (-5756.00 ± 8156.19 mm2) compared to PRT (-1552.54 ± 3896.58 mm2) (p=0.042; ES=0.66 (-0.01 – 1.31), but no difference was demonstrated among the other interventions (p>0.066). All elbows (100%) that received IASTM showed improved tissue orientation, 77% in the PRT group, 64% in US and 64% in the combination group. Conclusion: Manual therapy, particularly PRT and IASTM, seem to be better at increasing blood flow and muscle fiber orientation, respectively. Level of Evidence: II.

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Ferreira, Mónica V., Wilhelm Jahnen-Dechent i Sabine Neuss. "Standardization of Automated Cell-Based Protocols for Toxicity Testing of Biomaterials". Journal of Biomolecular Screening 16, nr 6 (25.04.2011): 647–54. http://dx.doi.org/10.1177/1087057111405380.

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Advances in high-throughput screening (HTS) instrumentation have led to enormous reduction of costs (e.g., of pipetting stations) and to the development of smaller instruments for automation of day-to-day routines in small research laboratories. In the biomaterials community, there has been an increasing interest for standardized screening protocols to identify cell type–specific cytocompatible biomaterials suitable for tissue engineering (TE) applications. In this study, the authors established a multiplexed assay protocol for toxicity screening of biomaterials using a low- to medium-throughput robotic liquid handling station (LHS). The protocol contains analysis of viability, cytotoxicity, and apoptosis combined in one assay. This study includes performance results of a side-by-side comparison of the EpMotion 5070 LHS and conventional pipetting/dispensing systems. Critical parameters were optimized each for a given platform. Higher accuracy and reproducibility were achieved for LHS compared to manually treated samples. The practicability and accuracy of the method in a typical small laboratory setting were tested by running daily routine tasks by trained and untrained laboratory staff. In addition, advantages and disadvantages as well as the step-by-step application protocol are reported. The approach described provides a potential utility in screening biomaterials toxicity, allowing researchers to meet the needs of low- and medium-throughput laboratories.

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Lee, H. P. "Comparison between Traditional and Web-Based Interactive Manuals for Laboratory-Based Subjects". International Journal of Mechanical Engineering Education 30, nr 4 (październik 2002): 307–14. http://dx.doi.org/10.7227/ijmee.30.4.3.

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This paper presents the salient features of web-based interactive manuals for laboratory-based subjects and compares them with traditional laboratory manuals. The manuals utilize the latest developments in web applications, JAVA programming and pre-designed spreadsheets with the objective of reducing the time taken for students to perform the mundane and laborious calculations and plotting of graphs. The students can therefore utilize the time in the laboratories on the analysis and discussion of results besides performing the actual experiments. Moreover, the web-based interactive manuals incorporate virtual experiments, digital pictures, animated GIF files and quick movies to familiarize the students with the experimental set-up before performing the physical experiments. The proposed approach is applicable for laboratory-based subjects, which are not amenable to be converted completely to virtual laboratories.

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Chou, P. H., Y. L. Chou, H. C. Wei, C. S. Ho i S. S. Jiang. "Biomechanical Analysis of Wrist Loading During Lifting Tasks". Journal of Mechanics 17, nr 4 (grudzień 2001): 179–87. http://dx.doi.org/10.1017/s172771910000191x.

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ABSTRACTThis study develops a kinematic/kinetic model to evaluate the effect of different handle angles on wrist loading during lifting task. An imaged-based motion analysis system is used to study the movement pattern, force and moment of the wrist joint among nine different handle angles. Six CCD cameras were used to record 3-D trajectories of limb-mounted markers based on the laboratory coordination system as defined by an 8-marker cube. Euler angles are used to describe the orientation of a distal segment reference frame relative to a proximal segment reference frame. Each segment of the upper extremities is regarded as a uniform rigid body with six degrees of freedom. The resultant loading of the wrist joints was determined using an inverse dynamic procedure.This study indicates that tool handles can be designed or selected to reduce manual loading and the potential for injury during tool use. The mean curve of joint force and moment provided consultations and understandings of the wrist loading during lifting task. In this study, handles that kept the wrist joints in a dorsiflexed and radial deviated position, showed significant reduction in stresses around the surrounding soft tissue.

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Kelder, Cindy, Astrid Bakker, Jenneke Klein-Nulend i Daniël Wismeijer. "The 3D Printing of Calcium Phosphate with K-Carrageenan under Conditions Permitting the Incorporation of Biological Components—A Method". Journal of Functional Biomaterials 9, nr 4 (17.10.2018): 57. http://dx.doi.org/10.3390/jfb9040057.

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Critical-size bone defects are a common clinical problem. The golden standard to treat these defects is autologous bone grafting. Besides the limitations of availability and co-morbidity, autografts have to be manually adapted to fit in the defect, which might result in a sub-optimal fit and impaired healing. Scaffolds with precise dimensions can be created using 3-dimensional (3D) printing, enabling the production of patient-specific, ‘tailor-made’ bone substitutes with an exact fit. Calcium phosphate (CaP) is a popular material for bone tissue engineering due to its biocompatibility, osteoconductivity, and biodegradable properties. To enhance bone formation, a bioactive 3D-printed CaP scaffold can be created by combining the printed CaP scaffold with biological components such as growth factors and cytokines, e.g., vascular endothelial growth factor (VEGF), bone morphogenetic protein-2 (BMP-2), and interleukin-6 (IL-6). However, the 3D-printing of CaP with a biological component is challenging since production techniques often use high temperatures or aggressive chemicals, which hinders/inactivates the bioactivity of the incorporated biological components. Therefore, in our laboratory, we routinely perform extrusion-based 3D-printing with a biological binder at room temperature to create porous scaffolds for bone healing. In this method paper, we describe in detail a 3D-printing procedure for CaP paste with K-carrageenan as a biological binder.

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Kyzlasov, P. S., F. G. Kolpacynidi, D. V. Kazantsev, V. I. Doga, A. N. Bashkov i O. V. Parinov. "NON-TRAUMATIC FORNIX RUPTURE WITH CONTRAST EXTRAVASATION". MEDICAL RADIOLOGY AND RADIATION SAFETY 67, nr 2 (kwiecień 2022): 73–75. http://dx.doi.org/10.33266/1024-6177-2022-67-2-73-75.

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Purpose: Description of a clinical case of surgical treatment of nontraumatic rupture of the renal calyx fornix, with extravasation of contrast agent into the perirenal tissue. Material and methods: Provided surgical treatment to a patient with spontaneous rupture of the calyx fornix on the background of ureteral obstruction caused by a stone. Results: Considering the concomitant pathology in the form of obstructive pyelonephritis, it was decided to drain the upper urinary tract with an external stent. After 7 days, the patient underwent replacement of the external stent with the internal one. The patient underwent antibacterial, anti-inflammatory therapy, with a positive clinical, laboratory effect. Retrograde pyelography was performed 7 days later. Violations of the integrity of the cavity system of the left kidney were not revealed. The external stent was replaced with an internal one. After 2 months, ureterolitho-extraction was performed on the left. Conclusion: From the data of the world literature and our own experience, we can conclude that spontaneous, non-traumatic rupture of the fornix is an extremely rare, urgent urological disease. We assume that the development of this process is similar to the Burhave syndrome, since smooth muscle fibers are present in the esophagus as well as in the esophagus. In order to provide immediate assistance to such patients, it is necessary to urgently drain the upper urinary tract. Timely diagnosis of this condition requires rapid tactics in the form of immediate ultrasound, radiological diagnostic methods, as well as computed tomography. The operational manual on the removal of stones in this case should be postponed for 2–3 weeks.

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Hj. Idrus, Ruszymah, i Aminuddin Saim. "Tissue Engineering Laboratory in Malaysia". Asia-Pacific Biotech News 09, nr 14 (30.07.2005): 664–67. http://dx.doi.org/10.1142/s0219030305001886.

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Alhudaithy, Soliman, Sama Abdulmalik, Sangamesh G. Kumbar i Kazunori Hoshino. "Design, Fabrication, and Validation of a Petri Dish-Compatible PDMS Bioreactor for the Tensile Stimulation and Characterization of Microtissues". Micromachines 11, nr 10 (26.09.2020): 892. http://dx.doi.org/10.3390/mi11100892.

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In this paper, we report on a novel biocompatible micromechanical bioreactor (actuator and sensor) designed for the in situ manipulation and characterization of live microtissues. The purpose of this study was to develop and validate an application-targeted sterile bioreactor that is accessible, inexpensive, adjustable, and easily fabricated. Our method relies on a simple polydimethylsiloxane (PDMS) molding technique for fabrication and is compatible with commonly-used laboratory equipment and materials. Our unique design includes a flexible thin membrane that allows for the transfer of an external actuation into the PDMS beam-based actuator and sensor placed inside a conventional 35 mm cell culture Petri dish. Through computational analysis followed by experimental testing, we demonstrated its functionality, accuracy, sensitivity, and tunable operating range. Through time-course testing, the actuator delivered strains of over 20% to biodegradable electrospun poly (D, L-lactide-co-glycolide) (PLGA) 85:15 non-aligned nanofibers (~91 µm thick). At the same time, the sensor was able to characterize time-course changes in Young’s modulus (down to 10–150 kPa), induced by an application of isopropyl alcohol (IPA). Furthermore, the actuator delivered strains of up to 4% to PDMS monolayers (~30 µm thick), simultaneously characterizing their elastic modulus up to ~2.2 MPa. The platform repeatedly applied dynamic (0.23 Hz) tensile stimuli to live Human Dermal Fibroblast (HDF) cells for 12 hours (h) and recorded the cellular reorientation towards two angle regimes, with averages of −58.85° and +56.02°. The device biocompatibility with live cells was demonstrated for one week, with no signs of cytotoxicity. We can conclude that our PDMS bioreactor is advantageous for low-cost tissue/cell culture micromanipulation studies involving mechanical actuation and characterization. Our device eliminates the need for an expensive experimental setup for cell micromanipulation, increasing the ease of live-cell manipulation studies by providing an affordable way of conducting high-throughput experiments without the need to open the Petri dish, reducing manual handling, cross-contamination, supplies, and costs. The device design, material, and methods allow the user to define the operational range based on their targeted samples/application.

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Lyubaeva, E. V., V. A. Vankov, T. V. Moiseeva i I. I. Markov. "ARE THE MEGAKARYOCYTES OF THE SPLEEN OF WHITE LABORATORY RATS IDENTICAL TO MEGACARIOCYTES OF THE BONE MARROW?" Morphological newsletter 25, nr 3 (30.09.2017): 69–73. http://dx.doi.org/10.20340/mv-mn.17(25).03.69-73.

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Many aspects of spleen histophysiology in humans and mammals still remain unsolved until today. This includes the spleen in white rats. The presence of megakaryocytes in its parenchyma has been only briefly indicated in some papers. As for appropriate information, let alone illustration, it is not available even in manuals on histology or the current histological atlases. The aim of the study is to obtain comparative morphological data on the spleen and marrow megakaryocytes in white rats. The object of this study is the spleen and marrow of the mandibular bone of outbred white rats (n=17) weighing 180,0-200,0 g. Staining the spleen preparations and mandibular trabecular bone tissue paraffin sections with hematoxylin and eosin, Azur-II-eosin and according to Van Gieson. In the spleen of white rats, megakaryocytes they appear identical to the marrow megakaryocytes.

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Howard, Mark. "Tissue Engineering—Essentials for Daily Laboratory Work". Annals of Biomedical Engineering 34, nr 1 (8.03.2006): 185. http://dx.doi.org/10.1007/s10439-005-9016-1.

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Dosh, Rasha Hatem, Nicola Jordan-Mahy, Christopher Sammon i Christine Lyn Le Maitre. "Tissue Engineering Laboratory Models of the Small Intestine". Tissue Engineering Part B: Reviews 24, nr 2 (kwiecień 2018): 98–111. http://dx.doi.org/10.1089/ten.teb.2017.0276.

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Smirnov, I., B. Nasibullin i S. Gushcha. "Assessment of the state of synergies between the structural and functional characteristics of the kidneys and liver during external use of a seaweed product Laminaria algae". Journal of Education, Health and Sport 52 (31.01.2024): 215–28. http://dx.doi.org/10.12775/jehs.2024.52.108.

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The authors conducted studies to study the relationship between the functional activity of the kidneys, the detoxification activity of the liver and the structural features of the parenchymatous tissue of these organs under normal conditions and with external use of the laminar algae product “Lamidan”. The work was performed on 164 white outbred Wistar rats weighing 180-200 g. The work was carried out in accordance with current standards for working with laboratory animals. The results of many years of research have shown that normally the indicators of detoxification activity of the liver and functional activity of the kidneys practically do not change, and they correspond to the structural organization of the parenchymatous tissue of these organs, corresponding to the descriptions given in well-known manuals on the activities of these organs. The use of "Lamidan" activates the detoxification function of the liver by 28 % and the functional activity of the kidneys by 28.5 %, i.e. changes are unidirectional and proportionate. In the parenchyma of both organs, changes in the structural organization are recorded, characteristic of an increase in functional activity. Thus, there is a strictly interrelated set of stereotypical changes in the indices of the two organs under study, reflecting between the organs interrelations of functional systems.

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Aliyo, Alqeer, i Alo Edin. "Assessment of Safety Requirements and Their Practices Among Teaching Laboratories of Health Institutes". Microbiology Insights 16 (styczeń 2023): 117863612311744. http://dx.doi.org/10.1177/11786361231174414.

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Background: Laboratory work provides students with the ability to process skills, perform experiments and tests, and interpret experimental data. Instead of traditional teaching methods, there are good laboratory experiences to develop a conceptual understanding of science. A lack of laboratory safety requirements and practice can affect the health of students, staff, and the environment. Therefore, the current study provides updated safety requirements and practice information. Objective: The purpose of this study was to evaluate safety requirements and practices among teaching laboratories at the Health Institute 2021. Methods: An institutional-based descriptive study design was conducted on November 15-20, 2020, among staff members of the Bule Hora University Institute of Health. Seventeen randomly selected academic staffs and laboratory assistants from 2 departments participated in the study. Data were collected through a self-administered questionnaire and an observational checklist. Finally, the data were coded and entered into Statistical Package for Social Sciences (SPSS) version 20 for analysis. Data were then analyzed using simple quantitative descriptions such as frequency counts and percentages. The data are presented in a table. Results: Of the safety requirements evaluated, only 33.3% (6) were available in the laboratory. Of the safety practices evaluated, 44.6% were used all the time, 37.7% were used occasionally, and 17.6% were never practiced in the laboratory by the respondents. Of his respondents, 58.8% had never been regularly inspected for laboratory safety, and 77% had never received training in laboratory safety. Observations show that teaching laboratories in health organizations lack safety manuals/first aid diaries or guidelines, laboratory building drainage systems, adequate ventilation, water flow, and appropriate laboratory sizes. Conclusions: This study reveals poor laboratory safety practices and safety requirements in teaching laboratories. These limitations may cause health problems, environmental pollution, contamination, and chemical spills. Stakeholders should improve safety requirements and create awareness among staff, students, and lab assistants.

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KUBO, HIROSHI. "Tissue engineering for pulmonary diseases: Insights from the laboratory". Respirology 17, nr 3 (23.03.2012): 445–54. http://dx.doi.org/10.1111/j.1440-1843.2012.02145.x.

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Bove, Mary, Annalisa Carlucci, Giovanni Natale, Chiara Freda, Antonio Noro, Vincenzo Ferrara, Giorgia Opromolla i in. "Tissue Engineering in Musculoskeletal Tissue: A Review of the Literature". Surgeries 2, nr 1 (28.01.2021): 58–82. http://dx.doi.org/10.3390/surgeries2010005.

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Tissue engineering refers to the attempt to create functional human tissue from cells in a laboratory. This is a field that uses living cells, biocompatible materials, suitable biochemical and physical factors, and their combinations to create tissue-like structures. To date, no tissue engineered skeletal muscle implants have been developed for clinical use, but they may represent a valid alternative for the treatment of volumetric muscle loss in the near future. Herein, we reviewed the literature and showed different techniques to produce synthetic tissues with the same architectural, structural and functional properties as native tissues.

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Mehta, Dr Rashesh P., Sushil Joshi, Pankaj Savani, Aditya Danayak i Mitul Munjani. "Development of Numerical Protection Laboratory through Industry Institute Interaction". Journal of Engineering Education Transformations 36, nr 2 (1.10.2022): 101–8. http://dx.doi.org/10.16920/jeet/2022/v36i2/22158.

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Abstract—The development of a numerical protection laboratory is implemented through industry-institute interaction under an MOU. This paper documents the contribution of the major project of final year students on using a digital over-current and earth-fault relay. The latest numerical protection relay has been provided as a replacement for an older version by ABB India Ltd. as a part of corporate social responsibility (CSR). The laboratory experimental panel has been developed through the major project of the final year student group. There was the active participation of ABB India Ltd. by way of providing dedicated training to the student group and faculty members involved in the installation, commissioning, and design of the experimental panel. The results obtained from various case studies are reported in this paper. The facilities created are used for regular laboratory practicals for undergraduate and postgraduate students. The laboratory manuals are prepared to facilitate the implementation of the experiments. The laboratory curriculum has been updated for the UG course "Power System Protection" and the PG course "Advanced Power System Protection and Switchgear". It has resulted in improvement in the placement of UG and PG students. The training of industry and state power utility engineers can also be performed on the experimental panel developed. The outcome of this academic innovation related to industry-institute interaction is that the industry readiness of students has increased. Keywords : Industry Institute Interaction; Power system protection; laboratory development; Test setup; Over-current protection.

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AS, Halim, Mohaini M i Chin Keong L. "ADIPOSE-DERIVED STEM CELLS IN TISSUE ENGINEERING: LABORATORY TO BEDSIDE". Journal of Health and Translational Medicine 16, nr 2 (30.12.2013): 1–10. http://dx.doi.org/10.22452/jummec.vol16no2.1.

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Bodnar, Cheryl, Thomas R. Christiani, Kevin Dahm i Andrea J. Vernengo. "Implementation and assessment of an undergraduate tissue engineering laboratory course". Education for Chemical Engineers 24 (lipiec 2018): 52–59. http://dx.doi.org/10.1016/j.ece.2018.07.002.

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Creager, Angela N. H. "Recipes for recombining DNA: A history of Molecular Cloning: A Laboratory Manual". BJHS Themes 5 (2020): 225–43. http://dx.doi.org/10.1017/bjt.2020.5.

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AbstractLaboratory instructions and recipes are sometimes edited into books with a wide circulation. Even in the late twentieth century, publications of this nature remained influential. For example, protocols from a 1980 summer course on gene cloning at Cold Spring Harbor Laboratory provided the basis for a bestselling laboratory manual by Tom Maniatis, Ed Fritsch and Joe Sambrook. Not only did the Molecular Cloning: A Laboratory Manual become a standard reference for molecular biologists (commonly called the ‘bible’), but also its recipes and clear instructions made gene cloning and recombinant DNA technologies accessible to non-specialists. Consequently, this laboratory manual contributed to the rapid spread of genetic-engineering techniques throughout the life sciences, as well as in industry. As is often the case with how-to books, however, finding a way to update methods in this rapidly changing field posed a challenge, and various molecular-biology reference books had different ways of dealing with knowledge obsolescence. This paper explores the origins of this manual, its publication history, its reception and its rivals – as well as the more recent migration of such laboratory manuals to the Internet.

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Balid, Walid, Mahmoud Abdulwahed i Imad Alrouh. "Development of an educationally oriented open-source embedded systems laboratory kit: A hybrid hands-on and virtual experimentation approach". International Journal of Electrical Engineering & Education 51, nr 4 (październik 2014): 340–53. http://dx.doi.org/10.7227/ijeee.0006.

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Embedded microcontroller (MCU) systems is one of the most important topics in undergraduate electrical and electronics engineering and computer engineering curricula. Laboratory sessions are vitally important in teaching/learning of MCUs. Unfortunately, most commercially available MCU development kits are not well designed for educational purposes. In this paper, we report on the design and implementation of an educationally oriented MCU kit. The design aimed to produce a fairly universal training board that can cover a range of experiments for different topics, which resulted in embedding a rich group of peripherals. Furthermore, the kit was associated with student-centric lab manuals, training exercise, video materials, and virtual MCU experiments. This paper presents a pedagogical investigation of the impact of using the embedded systems virtual labs for preparation. The quantitative results show statistical evidence that preparation with a virtual embedded systems lab results in higher learning outcomes.

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Guller, A. E., P. N. Grebenyuk, A. B. Shekhter, A. V. Zvyagin i S. M. Deyev. "Bioreactor-Based Tumor Tissue Engineering". Acta Naturae 8, nr 3 (15.09.2016): 44–58. http://dx.doi.org/10.32607/20758251-2016-8-3-44-58.

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This review focuses on modeling of cancer tumors using tissue engineering technology. Tumor tissue engineering (TTE) is a new method of three-dimensional (3D) simulation of malignant neoplasms. Design and development of complex tissue engineering constructs (TECs) that include cancer cells, cell-bearing scaffolds acting as the extracellular matrix, and other components of the tumor microenvironment is at the core of this approach. Although TECs can be transplanted into laboratory animals, the specific aim of TTE is the most realistic reproduction and long-term maintenance of the simulated tumor properties in vitro for cancer biology research and for the development of new methods of diagnosis and treatment of malignant neoplasms. Successful implementation of this challenging idea depends on bioreactor technology, which will enable optimization of culture conditions and control of tumor TECs development. In this review, we analyze the most popular bioreactor types in TTE and the emerging applications.

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Ott, Lindsey M., Robert A. Weatherly i Michael S. Detamore. "Overview of Tracheal Tissue Engineering: Clinical Need Drives the Laboratory Approach". Annals of Biomedical Engineering 39, nr 8 (19.05.2011): 2091–113. http://dx.doi.org/10.1007/s10439-011-0318-1.

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Galbusera, Fabio, Margherita Cioffi i Manuela T. Raimondi. "An in silico bioreactor for simulating laboratory experiments in tissue engineering". Biomedical Microdevices 10, nr 4 (31.01.2008): 547–54. http://dx.doi.org/10.1007/s10544-008-9164-9.

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Zimmermann, Elizabeth A., Imke A. K. Fiedler i Björn Busse. "Breaking new ground in mineralized tissue: Assessing tissue quality in clinical and laboratory studies". Journal of the Mechanical Behavior of Biomedical Materials 113 (styczeń 2021): 104138. http://dx.doi.org/10.1016/j.jmbbm.2020.104138.

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Vasyutin, I. A., A. V. Lyundup, A. Z. Vinarov, D. V. Butnaru i S. L. Kuznetsov. "Urethra Reconstruction with Tissue-Engineering Technology". Annals of the Russian academy of medical sciences 72, nr 1 (14.02.2017): 17–25. http://dx.doi.org/10.15690/vramn771.

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Urethral stricture is a disease characterized by a pathological narrowing of the urethra. Treatment for this condition often requires surgery using autologous grafts (urethroplasty). It is common practice to use patient’s own tissue like genital and extragenital skin, tunica vaginalis, buccal mucosa as a source of the graft. Alternative and safer approach is to use tissue-engineered graft created in a laboratory using patient’s autologous cells and biocompatible matrix (scaffold). The article presents the up-to-date achievements in lab-created tissue-engineered graft, describes all components needed to build a tissue-engineered structure of the graft for urethroplasty, and summarizes authors’ thoughts on advantages and disadvantages of various approaches to choose both cellular component and the matrix of future construction. The article reviews clinical studies conducted in the field of tissue engineering of the graft material for urethraplasty.

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Olofsson, Karl, Björn Hammarström i Martin Wiklund. "Ultrasonic Based Tissue Modelling and Engineering". Micromachines 9, nr 11 (14.11.2018): 594. http://dx.doi.org/10.3390/mi9110594.

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Systems and devices for in vitro tissue modelling and engineering are valuable tools, which combine the strength between the controlled laboratory environment and the complex tissue organization and environment in vivo. Device-based tissue engineering is also a possible avenue for future explant culture in regenerative medicine. The most fundamental requirements on platforms intended for tissue modelling and engineering are their ability to shape and maintain cell aggregates over long-term culture. An emerging technology for tissue shaping and culture is ultrasonic standing wave (USW) particle manipulation, which offers label-free and gentle positioning and aggregation of cells. The pressure nodes defined by the USW, where cells are trapped in most cases, are stable over time and can be both static and dynamic depending on actuation schemes. In this review article, we highlight the potential of USW cell manipulation as a tool for tissue modelling and engineering.

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Fiegel, Henning C., Peter M. Kaufmann, Helge Bruns, Dietrich Kluth, Raymund E. Horch, Joseph P. Vacanti i Ulrich Kneser. "Tissue Engineering Review Series: Hepatic tissue engineering: from transplantation to customized cell-based liver directed therapies from the laboratory". Journal of Cellular and Molecular Medicine 12, nr 1 (24.09.2007): 56–66. http://dx.doi.org/10.1111/j.1582-4934.2007.00162.x.

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Dunn, Michael G. "Tissue-Engineering Strategies for Ligament Reconstruction". MRS Bulletin 21, nr 11 (listopad 1996): 43–46. http://dx.doi.org/10.1557/s0883769400031845.

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The majority of musculoskeletal injuries involve the soft connective tissues including cartilage, meniscus, tendon, and ligament. In contrast to bone tissue, which regenerates after injury, these soft tissues tend to heal poorly through formation of weak, disorganized scar tissue. In fact certain soft-tissue injuries cannot heal on their own so surgical repair or complete reconstruction is required to restore function. Biological grafts (autografts and allografts) and “permanent” synthetic prostheses are currently used to reconstruct large soft-tissue defects. Autografts (a patient's own tissue transferred to another site) can be successful, but as you “rob Peter to pay Paul,” complications at the donor site can be significant. Allografts (transplants from another human) are in short supply and carry the risk of rejection or disease transmission. In general, permanent synthetic prostheses fail to withstand long-term mechanical loading associated with the musculoskeletal system. New strategies are clearly needed to manage musculoskeletal soft-tissue reconstruction.Several tissue-engineering strategies are potentially useful to enhance repair of these troublesome injuries or to induce regeneration. One interesting approach (developed for skin and cartilage regeneration) combines scaffolds, cells, and cell signals to grow living tissue analogues in the laboratory. Scaffolds are natural or synthetic biomaterials upon which the cells attach and grow. The cells may be fibroblasts, chondrocytes, etc., depending on the specific application. Influential cell signals can include attachment factors, growth factors, and mechanical loads.Our laboratory is developing a similar approach for ligament reconstruction. Ligaments are the strong, flexible bands of collagenous tissue that connect bone-to-bone to provide a delicate balance of stability and flexibility to the joints of the body. The anterior cruciate ligament (ACL) is the primary stabilizer of the knee and is frequently injured in sporting activities and accidents. Serious ACL injuries can lead to disability and progressive degeneration of cartilage and meniscus that normally serve as shock absorbers in the joint. Anterior-cruciate-ligament ruptures do not heal without surgical intervention, due to a poor intrinsic healing response and poor vascularity. To restore normal joint function, ruptured ACLs can be surgically reconstructed using a patellar-tendon autograft or allograft. While significant progress has been made in understanding ACL anatomy, structure, biomechanics, and healing, there is still no biological graft or biomaterial ideally suited for ACL reconstruction.

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Jian, Mariana, Vitalie Cobzac i Viorel Nacu. "Patented Devices and Methods for Utilization in Tissue Engineering". Intllectus, nr 1 (lipiec 2022): 94–100. http://dx.doi.org/10.56329/1810-7087.22.1.09.

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This paper describes the patented methods and devices which were obtained during researches in projects conducted in the Laboratory of Tissue Engineering and Cell Cultures of SUMPh “N. Testemitanu”. Patented devices and methods are part of tissue engineering domain and are of economic, technical and medical importance, because they allow an efficient, safe and qualitative process for grafts obtaining for transplantation.

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Yilgor, Caglar, Pinar Yilgor Huri i Gazi Huri. "Tissue Engineering Strategies in Ligament Regeneration". Stem Cells International 2012 (2012): 1–9. http://dx.doi.org/10.1155/2012/374676.

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Ligaments are dense fibrous connective tissues that connect bones to other bones and their injuries are frequently encountered in the clinic. The current clinical approaches in ligament repair and regeneration are limited to autografts, as the gold standard, and allografts. Both of these techniques have their own drawbacks that limit the success in clinical setting; therefore, new strategies are being developed in order to be able to solve the current problems of ligament grafting. Tissue engineering is a novel promising technique that aims to solve these problems, by producing viable artificial ligament substitutes in the laboratory conditions with the potential of transplantation to the patients with a high success rate. Direct cell and/or growth factor injection to the defect site is another current approach aiming to enhance the repair process of the native tissue. This review summarizes the current approaches in ligament tissue engineering strategies including the use of scaffolds, their modification techniques, as well as the use of bioreactors to achieve enhanced regeneration rates, while also discussing the advances in growth factor and cell therapy applications towards obtaining enhanced ligament regeneration.

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Sun, Yen, Hsin-Yuan Tan, Sung-Jan Lin, Hsuan-Shu Lee, Tzu-Yu Lin, Shiou-Hwa Jee, Tai-Horng Young, Wen Lo, Wei-Liang Chen i Chen-Yuan Dong. "Imaging tissue engineering scaffolds using multiphoton microscopy". Microscopy Research and Technique 71, nr 2 (luty 2008): 140–45. http://dx.doi.org/10.1002/jemt.20537.

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Lagasse, P. F., E. V. Richardson i J. D. Schall. "Fixed Instrumentation for Monitoring Scour at Bridges". Transportation Research Record: Journal of the Transportation Research Board 1647, nr 1 (styczeń 1998): 1–9. http://dx.doi.org/10.3141/1647-01.

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Research was conducted to develop, test, and evaluate fixed instrumentation that would be both technically and economically feasible for use in measuring maximum scour depth at bridge piers and abutments. A variety of scour-measuring and scour-monitoring methods were tested in the laboratory and in the field, including sounding rods, driven-rod devices, sonic depth finders (fathometers), and buried devices. Two fixed-instrument systems, a low-cost fathometer, and a magnetic slidingcollar device using a driven-rod approach, showed significant promise during initial testing. The project concentrated on installing and testing these two instrument systems under a wide range of bridge substructure geometry, flow, and geomorphic conditions. Both instrument systems met all of the mandatory and many of the desirable criteria established for this research. Cooperative efforts with state highway agencies proved that both systems can be installed with equipment and technical skills normally available to district-level department of transportation maintenance and inspection personnel. The project also provided installation, operation, and fabrication manuals for the low-cost sonic instrument system and magnetic sliding-collar devices.

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Witczak, M. W., i O. A. Fonseca. "Revised Predictive Model for Dynamic (Complex) Modulus of Asphalt Mixtures". Transportation Research Record: Journal of the Transportation Research Board 1540, nr 1 (styczeń 1996): 15–23. http://dx.doi.org/10.1177/0361198196154000103.

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Many predictive techniques for determining the dynamic modulus of asphalt concrete mixes have evolved over the past 25 years. One such model, developed at the University of Maryland, has been widely incorporated in design manuals and methods used throughout the world. However, the current predictive model only allows the determination of the dynamic modulus from the original bitumen properties among other variables. This model has been developed from dynamic (complex) modulus tests on laboratory-prepared specimens and does not take into account the hardening effects that take place during short- and long-term aging. By incorporating recent field studies on the aged viscosity of conventional asphalt cements, a revised model for the dynamic modulus of asphalt mixtures has been developed using the actual bitumen viscosity as the most important predictor variable in place of temperature. This modification now allows the model to be used to predict dynamic modulus for mixtures exhibiting any degree of binder aging. Also, by using a sigmoidal function model form, significant improvement in prediction rationality was achieved for using the model at extreme temperature conditions.

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ANENE-NZELU, CHUKWUEMEKA, YAN WANG, HANRY YU i LEO HWA LIANG. "LIVER TISSUE MODEL FOR DRUG TOXICITY SCREENING". Journal of Mechanics in Medicine and Biology 11, nr 02 (kwiecień 2011): 369–90. http://dx.doi.org/10.1142/s0219519411004083.

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Understanding the mechanisms involved in the biotransformation of new drugs and their toxicological implications is important for drug development. In this regard, a lot of effort has been put into research to recreate the liver tissue in the laboratory for the purpose of drug screening. This has also helped to minimize the use of laboratory animal and reduce incidence of post-market withdrawal of drugs. Despite the progress made so far, cell source remains a major limitation since primary human hepatocytes are scarce and the various cell alternatives do not express all the genes found in the normal liver. In terms of tissue construct, there is a current shift to 3D models since the cell–cell interactions found in the 3D configuration enhance the morphology and function of hepatocytes. Furthermore, the engineered tissue's performance can be optimized by cocultures, perfusion-based systems, and the use of scaffolds. Nanotechnology seems promising in the field of tissue engineering, as it has been proven that cell–matrix interactions at the nano level can influence greatly on the outcome of the tissue. The review explores the various cell sources, the 3D model, flow-based systems, cocultures, and nanoscaffolds use in hepatocytes in vitro drug testing

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Sonawane, Harshal. "College Management Web Application System Using Mean Stack". International Journal for Research in Applied Science and Engineering Technology 10, nr 6 (30.06.2022): 1849–55. http://dx.doi.org/10.22214/ijraset.2022.44096.

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Abstract: The main goal of this project is to add mobility and automation to the process of managing student information in the laboratory. In real-world scenarios, for example, on a university campus, information is distributed to students in the form of notifications, handwritten manuals, and word-of-mouth messages. Today, it is important to communicate faster and easier between students using new formats such as mobile phone technology, as well as predictable forms of expression.The central idea of this project is the implementation of a web-based campus application for further development of educational institutions and educational systems. This application is used by students, teachers and parents. In previous systems, all information had to be displayed in a hard file or website. At the same time, searching for information is difficult to access and takes a long time to search existing websites. Therefore, to solve this problem, you can use a Web based application with MEAN Stack to make this process easier, more secure, and less error-prone. This system provides more efficient information.

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Thakur, Shivani, Shruti, Sohin Hashmi, Saket Mishra, Shashi Kant Ekka, Akhilesh Kushwaha i Reena Kujur. "A Review on Plant Tissue Culture". Asian Journal of Biology 20, nr 2 (15.02.2024): 14–18. http://dx.doi.org/10.9734/ajob/2024/v20i2387.

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Plant tissue and cell culture involve the aseptic cultivation and propagation of plant cells, tissues, and organs in a meticulously controlled laboratory setting. This innovative method harnesses the potential of nutrient-rich media to efficiently replicate plant cells on a large scale, resulting in the rapid production of mature and disease-free plants. The cornerstone of commercial technology in this field is micropropagation, a process that achieves swift proliferation from minute plant cuttings, axillary buds, and, to a limited extent, from somatic embryos and cell clumps in suspension cultures and bioreactors. Micropropagation, a pivotal aspect of plant tissue and cell culture, holds immense value in generating high-quality and consistent planting materials. These materials find applications across diverse fields, including molecular genetic engineering, plant breeding, horticulture production, and environmental preservation. The process of micropopagation unfolds through several distinct stages, including propagation, subculture of explants for proliferation, shooting and rooting, and hardening. These stages collectively form a universal framework for large-scale multiplication of plants. This technique plays a critical role in overcoming the limitations of traditional plant propagation methods. By allowing for controlled and accelerated growth in a laboratory environment, plant tissue and cell culture contribute to the efficient production of disease-resistant and genetically uniform plant materials. This not only supports advancements in various scientific disciplines but also addresses practical needs in agriculture, horticulture, and environmental conservation. The continuous refinement and application of plant tissue and cell culture methods underscore their significance in meeting the growing demands for sustainable and high-quality plant materials across diverse sectors.

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Manohar, Srirang, Alexei Kharine, Johan C. G. van Hespen, Wiendelt Steenbergen i Ton G. van Leeuwen. "Photoacoustic mammography laboratory prototype: imaging of breast tissue phantoms". Journal of Biomedical Optics 9, nr 6 (2004): 1172. http://dx.doi.org/10.1117/1.1803548.

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Gupta, Sweta, Chhavi Sharma, Amit Kumar Dinda, Amiya K. Ray i Narayan C. Mishra. "Tooth Tissue Engineering: Potential and Pitfalls". Journal of Biomimetics, Biomaterials and Tissue Engineering 12 (luty 2012): 59–81. http://dx.doi.org/10.4028/www.scientific.net/jbbte.12.59.

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All over the world a large number of people suffer from tooth diseases like dental caries, tooth abscess, and plaques. Tooth loss or damage, which occurs frequently in our society are generally repaired by applying several conventional methods, such as root-canal treatment, direct pulp capping and dental implants. These methods are quite painful, create damage to the surrounding tooth tissues and also may at times have adverse side-effects. The limitations of the conventional methods can be overcome by applying the concept of tooth tissue engineering. Tooth tissue engineering is the application of biosciences and engineering to regenerate a biofunctional tooth, which can be used to replace the missing tooth or repair the damaged tooth. Tissue engineering involves three key elements - cell, scaffold and growth factors, which interact with each other to regenerate a specific tissue. The success of tissue engineering depends on the proper selection of these three key elements and understanding the interactions among them. To bring us close to the realization of a tissue-engineered tooth, immense progress is going on in understanding how tooth is first developed, and there is a good advancement in tooth regeneration. In this review, “tooth tissue engineering” will be discussed, along with the recent advancements and challenges in bring a biofunctional tooth from laboratory out into clinical use.

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ANUKUL, PREEYANUCH, SOMWANG KHANTAYANUWONG i PHICHIT SOMBOON. "Development of laboratory wet creping method to evaluate and control pulp quality for tissue". May 2015 14, nr 5 (1.06.2015): 339–45. http://dx.doi.org/10.32964/tj14.5.339.

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The required properties of tissue paper including softness and liquid absorption are predominantly manipulated by the creping process, which cannot be simply produced in the laboratory. This causes problems in controlling the stock quality prepared using the various types of industrial pulps and consequently affects the creping operation and tissue quality. Our objectives were to develop a laboratory creping method and to apply it for the evaluation and control of the quality of pulp raw materials used in industrial tissue production. The experiment consisted of the development of a laboratory wet creping method and the study of the creping devices and the creping conditions, including basis weight of the base paper, sheet dryness, and degrees of pulp refining. The results showed that the method could produce crepes on laboratory sheets and the structures of the creped sheets could be engineered to improve their softness and liquid absorption. The tested pulps were found to produce significantly different responses to the laboratory wet creping, depending on their morphology and the mechanical treatments. The examination of industrial pulps found that eucalypt, bagasse, bamboo, and deinking pulps needed to be manipulated using different refining levels and required optimization among their softness, liquid absorption, and strength properties.

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Baum, B. J. "Prospects for Re-engineering Salivary Glands". Advances in Dental Research 14, nr 1 (grudzień 2000): 84–88. http://dx.doi.org/10.1177/08959374000140011401.

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In the last decade, two areas of biomedical research-gene therapy and tissue engineering-have especially captured the imagination of the public. Both areas offer the potential for the treatment of clinical conditions that now are considered impossible or extremely difficult to manage by conventional therapeutic measures. Gene therapy has made remarkable scientific progress in the laboratory, but has yet to realize its enormous clinical promise. Tissue engineering studies have led to some tangible clinical breakthroughs, but the routine replacement of whole internal organs is still well into the future. This report will examine the applications of gene therapy and tissue engineering to salivary glands, with a focus on the repair of irreversible gland damage.

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Mao, Yong, Tyler Hoffman, Amy Wu i Joachim Kohn. "An Innovative Laboratory Procedure to Expand Chondrocytes with Reduced Dedifferentiation". CARTILAGE 9, nr 2 (22.12.2017): 202–11. http://dx.doi.org/10.1177/1947603517746724.

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Objective In vitro expansion of chondrocytes is required for cartilage tissue engineering and clinical cell-based cartilage repair practices. However, the dedifferentiation of chondrocytes during in vitro expansion continues to be a challenge. This study focuses on identifying a cell culture surface to support chondrocyte expansion with reduced dedifferentiation. Design A less adhesive culture surface, non–tissue culture treated surface (NTC), was tested for its suitability for culturing chondrocytes. The cell expansion and the expression of chondrocyte markers were monitored for at least 2 passages on NTC in comparison with conventional tissue culture treated polystyrene surface (TCP). The ability of expanded chondrocytes to form cartilage tissues was evaluated using pellet culturing and subcutaneous implantation in nude mice. Results NTC supported bovine chondrocyte proliferation to a clinically relevant expansion requirement within 2 passages. Chondrocyte phenotypes were better maintained when cultured on NTC than on TCP. In vitro pellet culture studies showed that chondrocytes expanded on NTC expressed a higher level of chondrocyte extracellular matrix. Furthermore, the cells expanded on NTC or TCP were implanted subcutaneously as pellets in nude mice for 6 weeks. The recovered pellets showed cartilage-like tissue formation from cells expanded on NTC but not from the cells expanded on TCP. Conclusions This study presents an innovative and easy culturing procedure to expand chondrocytes with reduced dedifferentiation. This procedure has potential to be developed to expand chondrocytes in vitro for basic research, tissue engineering, and possibly for clinical applications.

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Haraguchi, Yuji, Tatsuya Shimizu, Masayuki Yamato i Teruo Okano. "Regenerative Therapies Using Cell Sheet-Based Tissue Engineering for Cardiac Disease". Cardiology Research and Practice 2011 (2011): 1–8. http://dx.doi.org/10.4061/2011/845170.

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At present, cardiac diseases are a major cause of morbidity and mortality in the world. Recently, a cell-based regenerative medicine has appeared as one of the most potential and promising therapies for improving cardiac diseases. As a new generational cell-based regenerative therapy, tissue engineering is focused. Our laboratory has originally developed cell sheet-based scaffold-free tissue engineering. Three-dimensional myocardial tissue fabricated by stacking cardiomyocyte sheets, which are tightly interconnected to each other through gap junctions, beats simultaneously and macroscopically and shows the characteristic structures of native heart tissue. Cell sheet-based therapy cures the damaged heart function of animal models and is clinically applied. Cell sheet-based tissue engineering has a promising and enormous potential in myocardial tissue regenerative medicine and will cure many patients suffering from severe cardiac disease. This paper summarizes cell sheet-based tissue engineering and its satisfactory therapeutic effects on cardiac disease.

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Lima, Marcus Vinicius Alves, Polliana Silva Rodrigues, João Otávio Ferreira Pereira, Caroline Felix da Silva Mota, Monica Nascimento Bittencourt, Winnie Revilane de Melo Marques, Cristina de Jesus Gomes i in. "Abnormal laboratory findings review in SARS-CoV-2 infection". Research, Society and Development 11, nr 5 (17.04.2022): e59611528793. http://dx.doi.org/10.33448/rsd-v11i5.28793.

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COVID-19 can trigger damage to the function and integrity of multiple cells, tissue or organs. In the progression of clinical manifestations, laboratory biomarkers can be used as predictors of prognosis to support in clinical conduction. This study aims to evidence an updated review of the literature of changes in biochemical and hematological laboratory tests of individuals with COVID-19 from articles prospected from the SciELO, LILACS and PubMed databases. Although in severe clinical spectrums, especially in patients admitted to intensive care unit, tissue lesions are more intense and more evident laboratory changes, can be observed in general in patients infected with SARS-CoV-2 increased levels of transaminases, alkaline phosphatase, gamma glutamyl transferase, bilirubin, urea, creatinine, lactic dehydrogenases, amylase, lipase, troponins, NT-ProBNP, pro-BNP, as well as reduction of MCV and MCHC, lymphopenia, neutrophilia, eosinopenia, thrombocytopenia, and prolongation of PT and aPTT.

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Sjöstrand, Björn, Mikael Danielsson i Magnus Lestelius. "Method for studying water removal and air penetration during through air drying of tissue in laboratory scale". BioResources 18, nr 2 (7.03.2023): 3073–88. http://dx.doi.org/10.15376/biores.18.2.3073-3088.

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Energy use, together with consumption of raw materials, machine clothing, and wet end chemicals, are some of the most critical aspects in successful tissue making today. This work was aimed at developing a laboratory-scale method of estimating dewatering mechanisms, vacuum efficiency, and energy use of Through Air Drying (TAD) of tissue. When compared to pilot data, the results of the new laboratory method for investigating dewatering during TAD were in the same magnitude, around 24 to 26% dryness after vacuum dewatering, and 27 to 29% dryness after TAD molding. Sheet properties, such as caliper and surface profile, were evaluated and compared to commercial tissue sheets. The results indicate that it will be possible to precisely measure accurate dryness development and penetrated air volume for tissue sheet forming and TAD molding at a laboratory scale. This can contribute to the efforts of implementing a circular forest-based bioeconomy by increasing the fundamental understanding of dewatering of tissue paper materials, which is facilitated by improvements in energy use. The new method developed in this work will make it easier to assess ideas that are difficult to bring to pilot scale or full scale before learning more of the dewatering capabilities. The authors are convinced that improved knowledge of tissue dewatering mechanisms, forming, and material transport during and after TAD dewatering can increase the efficiency of the industrial manufacturing processes.

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He, Yunfan, i Feng Lu. "Development of Synthetic and Natural Materials for Tissue Engineering Applications Using Adipose Stem Cells". Stem Cells International 2016 (2016): 1–12. http://dx.doi.org/10.1155/2016/5786257.

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Adipose stem cells have prominent implications in tissue regeneration due to their abundance and relative ease of harvest from adipose tissue and their abilities to differentiate into mature cells of various tissue lineages and secrete various growth cytokines. Development of tissue engineering techniques in combination with various carrier scaffolds and adipose stem cells offers great potential in overcoming the existing limitations constraining classical approaches used in plastic and reconstructive surgery. However, as most tissue engineering techniques are new and highly experimental, there are still many practical challenges that must be overcome before laboratory research can lead to large-scale clinical applications. Tissue engineering is currently a growing field of medical research; in this review, we will discuss the progress in research on biomaterials and scaffolds for tissue engineering applications using adipose stem cells.

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Simionescu, Agneta, Mary E. Tedder, Ting-Hsien Chuang i Dan T. Simionescu. "Lectin and antibody-based histochemical techniques for cardiovascular tissue engineering". Journal of Histotechnology 34, nr 1 (marzec 2011): 20–28. http://dx.doi.org/10.1179/014788811x12949268296040.

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Prezja, Fabi, Ilkka Pölönen, Sami Äyrämö, Pekka Ruusuvuori i Teijo Kuopio. "H&E Multi-Laboratory Staining Variance Exploration with Machine Learning". Applied Sciences 12, nr 15 (26.07.2022): 7511. http://dx.doi.org/10.3390/app12157511.

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In diagnostic histopathology, hematoxylin and eosin (H&E) staining is a critical process that highlights salient histological features. Staining results vary between laboratories regardless of the histopathological task, although the method does not change. This variance can impair the accuracy of algorithms and histopathologists’ time-to-insight. Investigating this variance can help calibrate stain normalization tasks to reverse this negative potential. With machine learning, this study evaluated the staining variance between different laboratories on three tissue types. We received H&E-stained slides from 66 different laboratories. Each slide contained kidney, skin, and colon tissue samples stained by the method routinely used in each laboratory. The samples were digitized and summarized as red, green, and blue channel histograms. Dimensions were reduced using principal component analysis. The data projected by principal components were inserted into the k-means clustering algorithm and the k-nearest neighbors classifier with the laboratories as the target. The k-means silhouette index indicated that K = 2 clusters had the best separability in all tissue types. The supervised classification result showed laboratory effects and tissue-type bias. Both supervised and unsupervised approaches suggested that tissue type also affected inter-laboratory variance. We suggest tissue type to also be considered upon choosing the staining and color-normalization approach.

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