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Artykuły w czasopismach na temat "T-RFLP"
Nagashima, Koji, Takayoshi Hisada, Maremi Sato i Jun Mochizuki. "Application of New Primer-Enzyme Combinations to Terminal Restriction Fragment Length Polymorphism Profiling of Bacterial Populations in Human Feces". Applied and Environmental Microbiology 69, nr 2 (luty 2003): 1251–62. http://dx.doi.org/10.1128/aem.69.2.1251-1262.2003.
Pełny tekst źródłaStępień-Słodkowska, Marta, Krzysztof Ficek, Mariusz Kaczmarczyk, Agnieszka Maciejewska-Karłowska, Marek Sawczuk, Agata Leońska-Duniec, Miłosz Stępiński i in. "The Variants Within the COL5A1 Gene are Associated with Reduced Risk of Anterior Cruciate Ligament Injury in Skiers". Journal of Human Kinetics 45, nr 1 (1.03.2015): 103–11. http://dx.doi.org/10.1515/hukin-2015-0011.
Pełny tekst źródłaASHER, A. J., L. S. WALDRON i M. L. POWER. "Rapid identification of Giardia duodenalis assemblages in NSW using terminal-restriction fragment length polymorphism". Parasitology 139, nr 8 (15.03.2012): 1005–13. http://dx.doi.org/10.1017/s0031182012000388.
Pełny tekst źródłaRåberg, Ulrika, Nils O. S. Högberg i Carl Johan Land. "Detection and species discrimination using rDNA T-RFLP for identification of wood decay fungi". Holzforschung 59, nr 6 (1.11.2005): 696–702. http://dx.doi.org/10.1515/hf.2005.111.
Pełny tekst źródłaLueders, Tillmann, i Michael W. Friedrich. "Evaluation of PCR Amplification Bias by Terminal Restriction Fragment Length Polymorphism Analysis of Small-Subunit rRNA and mcrA Genes by Using Defined Template Mixtures of Methanogenic Pure Cultures and Soil DNA Extracts". Applied and Environmental Microbiology 69, nr 1 (styczeń 2003): 320–26. http://dx.doi.org/10.1128/aem.69.1.320-326.2003.
Pełny tekst źródłaSeldin, M. F., H. C. Morse, J. P. Reeves, C. L. Scribner, R. C. LeBoeuf i A. D. Steinberg. "Genetic analysis of autoimmune gld mice. I. Identification of a restriction fragment length polymorphism closely linked to the gld mutation within a conserved linkage group." Journal of Experimental Medicine 167, nr 2 (1.02.1988): 688–93. http://dx.doi.org/10.1084/jem.167.2.688.
Pełny tekst źródłaDanovaro, R., G. M. Luna, A. Dell'Anno i B. Pietrangeli. "Comparison of Two Fingerprinting Techniques, Terminal Restriction Fragment Length Polymorphism and Automated Ribosomal Intergenic Spacer Analysis, for Determination of Bacterial Diversity in Aquatic Environments". Applied and Environmental Microbiology 72, nr 9 (wrzesień 2006): 5982–89. http://dx.doi.org/10.1128/aem.01361-06.
Pełny tekst źródłaJackson, Colin J., Richard C. Barton i E. Glyn V. Evans. "Species Identification and Strain Differentiation of Dermatophyte Fungi by Analysis of Ribosomal-DNA Intergenic Spacer Regions". Journal of Clinical Microbiology 37, nr 4 (1999): 931–36. http://dx.doi.org/10.1128/jcm.37.4.931-936.1999.
Pełny tekst źródłaPenny, Christian, Thierry Nadalig, Malek Alioua, Christelle Gruffaz, Stéphane Vuilleumier i Françoise Bringel. "Coupling of Denaturing High-Performance Liquid Chromatography and Terminal Restriction Fragment Length Polymorphism with Precise Fragment Sizing for Microbial Community Profiling and Characterization". Applied and Environmental Microbiology 76, nr 3 (30.11.2009): 648–51. http://dx.doi.org/10.1128/aem.01556-09.
Pełny tekst źródłaEgert, Markus, i Michael W. Friedrich. "Formation of Pseudo-Terminal Restriction Fragments, a PCR-Related Bias Affecting Terminal Restriction Fragment Length Polymorphism Analysis of Microbial Community Structure". Applied and Environmental Microbiology 69, nr 5 (maj 2003): 2555–62. http://dx.doi.org/10.1128/aem.69.5.2555-2562.2003.
Pełny tekst źródłaRozprawy doktorskie na temat "T-RFLP"
Rocha, Lidianne Leal. "Estudo de comunidades bacterianas de solos do Manguezal da Barra Grande, Icapuà â CE e SeleÃÃo de cepas com potencial para degradar hidrocarbonetos". Universidade Federal do CearÃ, 2008. http://www.teses.ufc.br/tde_busca/arquivo.php?codArquivo=2773.
Pełny tekst źródłaMendes, Lucas William. "Análise molecular das estruturas e diversidade de comunidades microbianas em solo de manguezal preservado da Ilha do Cardoso-SP". Universidade de São Paulo, 2009. http://www.teses.usp.br/teses/disponiveis/64/64133/tde-27042010-112316/.
Pełny tekst źródłaThe tropical mangroves are considered one of the most productive ecosystems of the world, being characterized by the high tax of organic matter and recycling of nutrients, that happens between the oceans and the terrestrial habitats. Although the mangroves are considered areas of environmental protection, the destruction of those ecosystems is progressive, due to industrial and port activities in the estuaries. In mangroves, the recycling of nutrients is directly related to the activities and to the diversity of microbial communities present in the soil. This work is part of a wider project inside of the program BIOTA/FAPESP, with respect to the studies of the biodiversity in the State of São Paulo and use of that biodiversity in a maintainable way. The objective of this work was to evaluate the structures and diversity of communities of Bacteria, Archaea and Fungi present in the soil of preserved mangrove of Ilha do Cardoso-SP. The samples were analyzed by T-RFLP and ARISA techniques, cloning and sequencing in order to obtain a characterization of the microbial communities structure of preserved mangrove area in comparison with the adjacent environments of restinga (tropical moist forest) and forest and also to a degraded mangrove. The results allowed concluding that the mangroves present exclusive characteristics, with the presence of distinct organisms, revealing a possible biotechnological potential to be explored. Additionally, the data revealed that the human action affected the structures of those communities in a way to be noticed a sensitive diversity decrease in the degraded mangrove, evidencing, this way, the importance of the ecosystem preservation
Fiore, Rebecca de Ara?jo. "Potencial de esp?cies florestais para remedia??o de substrato contaminado com atrazine e 2,4-D". UFVJM, 2014. http://acervo.ufvjm.edu.br:8080/jspui/handle/1/338.
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Funda??o de Amparo ? Pesquisa do estado de Minas Gerais (FAPEMIG)
Em se tratando da contamina??o de ecossistemas por res?duos de defensivos agr?colas, especial aten??o ? dada para a classe dos herbicidas, em fun??o do volume de aplica??o. Os que possuem mol?culas sol?veis pass?veis de contamina??o de len??is h?dricos subterr?neos se destacam pela abrang?ncia dos efeitos negativos. Nesse sentido, objetivou-se selecionar esp?cies vegetais arb?reas interessantes na remedia??o de ambientes contaminados por res?duos de atrazine e 2,4-D. Foram avaliados 36 tratamentos compostos pela combina??o de 12 esp?cies florestais [Inga marginata (ing?), Schizolobium parahyba (guapuruvu), Handroanthus serratifolius (ip? amarelo), Jacaranda puberula (carobinha), Cedrela fissilis (cedro), Calophyllum brasiliensis (landin), Psidium mirsinoides (goiabinha), Tibouchina glandulosa (quaresmeira), Caesalpinia f?rrea (pau-ferro), Caesalpinia pluviosa (sibipiruna), Terminalia arg?ntea (capit?o) e Schinopsis brasiliensis (bra?na)] e tr?s solu??es simulando o composto lixiviado (atrazine, 2,4-D e ?gua ? controle), com quatro repeti??es. Foram feitas 3 aplica??es dos herbicidas atrazine e 2,4-D com intervalos de 20 dias (aos 60, 80 e 100 dias ap?s o plantio), sendo cada aplica??o correspondente ? metade da dose comercial dos produtos. Para as avalia??es de crescimento foram mensuradas a altura da planta, o di?metro do caule, o n?mero de folhas, a ?rea foliar e o ac?mulo de biomassa seca. Na estimativa do efeito visual dos herbicidas ?s plantas avaliadas, optou-se pela atribui??o de notas em escala de intoxica??o. Para verifica??o de capacidade remediadora das esp?cies arb?reas procedeu-se a semeadura da esp?cie indicadora para indicativo do res?duo do herbicida no solo, (Cucumis sativus (L.)). Posteriormente em amostras de solo provenientes da esp?cie remediadora mais promissora procedeu-se a an?lise de polimorfismo de comprimento de fragmento de restri??o terminal (T-RFLP), com intuito de caracterizar a diversidade microbiana presente. As esp?cies florestais sobreviveram ? aplica??o dos herbicidas, sendo que umas se mostram mais sens?veis do que outras. O ing? apresentou bons resultados de remedia??o com o bioensaio, assim como o ip? amarelo, apesar da sua sensibilidade aos herbicidas. Observou-se aumento no conte?do relativo de macronutrientes para as plantas sob a??o dos herbicidas, na maioria dos tratamentos. Os resultados de T-RFLP confirmaram a diversidade microbiana diferenciada associada ? rizosfera de ing?, principalmente quando submetida ? a??o de atrazine.
Disserta??o (Mestrado) ? Programa de P?s-Gradua??o em Ci?ncia Florestal, Universidade Federal dos Vales do Jequitinhonha e Mucuri, 2014.
ABSTRACT In the case of contamination of ecosystems by residues of pesticides, special attention is given to the class of herbicides, according to the application volume. Those with likely contamination of underground water soluble molecules sheets are distinguished by breadth of negative effects. In this sense, the aim of this work was to select interesting woody plant species in the remediation of contaminated by residues of atrazine and 2,4-D environments. Were evaluated 36 treatments consisted of combinations of 12 forest species [Inga marginata (ing?), Schizolobium parahyba (guapuruvu), Handroanthus serratifolius (ip? amarelo), Jacaranda puberula (carobinha), Cedrela fissilis (cedro), Calophyllum brasiliensis (landin), Psidium mirsinoides (goiabinha), Tibouchina glandulosa (quaresmeira), Caesalpinia f?rrea (pau-ferro), Caesalpinia pluviosa (sibipiruna), Terminalia arg?ntea (capit?o) and Schinopsis brasiliensis (bra?na)] and three leachate solutions simulate the compound ( atrazine , 2,4 - D and water - control),with four replicates each. Three applications of atrazine and 2,4-D were made at intervals of 20 days (at 60, 80 and 100 days after planting), each application was corresponding to half of the recommended. Evaluations of growth were measured plant height, stem diameter, number of leaves, leaf area and dry biomass. In estimating the visual effect of herbicides on plants assessed, was opted for the grading scale of intoxication. To check remediation ability of tree species proceeded seeding indicator species for indication of herbicide residue in the soil (Cucumis sativus (L.)). Later in soil samples from the species most promising remedial proceeded to analysis of length polymorphism terminal restriction fragment (T-RFLP), in order to characterize the microbial diversity present. Forest species survived the herbicide application, and some are more sensitive than others. The ing? had good results with the remediation bioassay, as well as the ipe amarelo, despite their sensitivity to herbicides. Observed increase in the relative content of macronutrients for plants under the effect of herbicides in most treatments. The results of T-RFLP confirmed the differentiated microbial diversity associated with the rhizosphere of ing?, especially when subjected to the action of atrazine.
Santana, Maiele Cintra. "Análise da comunidade de fungos em áreas de monoculturas e consórcio de Eucalyptus grandis e Acacia mangium". Universidade de São Paulo, 2018. http://www.teses.usp.br/teses/disponiveis/11/11138/tde-03052018-173930/.
Pełny tekst źródłaThe fungi represent about 75% of the microbial biomass in forest areas, performing important functions, from the mineralization of the organic residues to the availability of nutrients to plants through mycorrhizal associations, which influences the nutrient cycling and, consequently, the growth of trees. The objective of this work was to evaluate the community of fungi of the soil, rhizosphere and root system of Eucalyptus grandis and Acacia mangium planted in monocultures and consortium, and to find explanations for the observed patterns through the correlation with physical and chemical soil attributes and soil depth. The samples were collected at the Experimental Station of Forest Sciences of Itatinga in 2016, when the plants were 2 years old. Samples were collected in four treatments: monocultures of E. grandis and A. mangium and consortia of E. grandis and A. mangium, in which trenches were constructed to collect samples in the 0-10, 10-20, 20 -50 and 50-100 cm deep. The physical and biological attributes of the soil and the chemical attributes of soil, rhizosphere and roots were characterized. For the mycorrhizal evaluation, the number of spores of arbuscular mycorrhizal fungi (AMF) and the rates of root colonization by AMF and ectomycorrhizal fungi were quantified. The morphology of arbuscular mycorrhizal and ectomycorrhizal (ECM) structures was evaluated. The structure of the soil and rhizosphere fungi community by was evaluated by the technique of Terminal restriction fragment length polymorphism (T-RFLP). For this, the DNA was amplified using primers ITS1f-FAM and ITS4 and restriction of the fragments was performed with the enzyme HaeIII. The abundance of ITS gene copies of soil and rhizosphere was quantified by quantitative PCR (qPCR), using primers ITS1f and 5.8s. The physical, chemical and biological attributes had few variations among the evaluated treatments, being the greatest differences found between the depths. The number of spores (<29) and mycorrhizal colonization rates (<48%) were low in all treatments, and reduced with increasing depth. A. mangium plants did not form FMA. In the roots of E. grandis, there was no formation of arbuscules, but we found the presence of hyphal coils, mycorrhizal structures of the Paris type. The anatomy of the ECM confirmed the colonization of these fungi in the roots of the studied plants. The qPCR showed higher abundance of ITS genes in the rhizosphere in relation to the soil, as well as in the superficial layers (0-10 cm) in relation to the deeper ones (10 cm below). The Principal Coordinates Analysis revealed differences in the structure of the fungal communities in the treatments studied, especially for the rhizosphere region, differentiating the fungal profile of the E. grandis monoculture from the other treatments, as well as the influence of A. mangium on the structure of the community. The redundancy analysis showed the influence of some chemical soil attributes on the rates of colonization and community structuring. Thus, it is concluded that in a consortium system, one plant species seems to be more influential than the other in structuring the fungal community, and this influence is more evident in the rhizosphere. In addition, chemical attributes are important factors in the organization of the fungal community.
Miao, Yu. "Development and use of T-RFLP for studies of take-all infection of wheat". Thesis, University of Nottingham, 2008. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.490991.
Pełny tekst źródłaFornazari, Anna Cristina Zari. "Determinação da comunidade microbiana pelo método molecular T-RFLP em carnes refrigeradas embaladas a vácuo". Universidade de São Paulo, 2011. http://www.teses.usp.br/teses/disponiveis/11/11141/tde-22112011-085341/.
Pełny tekst źródłaThe distension of the packaging of vacuum packed chilled meat, also know as blown pack, is assigned to psichrophilic and psychrotrophic bacteria, among which are part some clostridium, enterobacteria and lactic bacteria.The ability of these microorganisms to grow at refrigeration temperatures makes it an appropriate challenge its control by the industry. Brazil is a major producer of beef in the world and despite the importance of the beef industry represents for the country, there are few studies on the possible causes of deterioration of blown pack type. The importance of this work is based on the dearth of research on this problem that affects the meat industry. The aim of this study was to evaluate the presence of the main microorganisms involved in the deterioration blown pack type, with emphasis on clostridia, enterobacteria and lactic bacteria. Thus, the method-Terminal Restriction Fragment Length Polymorphism, available at Molecular biology laboratory of CENA-USP, was used to allow a rapid and accurate diagnosis for the detection of microorganisms. We analyzed 15 samples of beef chilled, vacuum packed wich abundant gas production, refrigerators from the states of São Paulo, Mato Grosso do Sul and Goiás cuts used for the analysis were striploin, shin, rump, hump, cop of rump and cube roll. The samples showing signs of deterioration such blown pack within the shelf life, with storage periods ranging from 30 to 120 days. Were identified Clostridium algidicarnis, Clostridium gasigenes, Clostridium putrefaciens, Clostridium frigidicarnis, Lactobacillus sakei, Hafnia alvei and Serratia liquefaciens by TRFLP technique, which proved an excellent tool for microbial identification in meat samples. The high prevalence of enterobacteria in samples of meat detected by the technique of TRFLP analysis was performed with conventional isolation and identification of enterobacteria, in order to confirm the presence of viable and culturable microorganisms in the samples. The species of enterobacteria were identified and cultivated H. alvei, Ser. liquefaciens, Citrobacter braakii, Pantoea sp. and Yersinia enterocolitica, the latter being potentially pathogenic and interest in public health. It was observed that H. alvei was the predominant species in the samples evaluated by both the T-RFLP technique and by conventional microbiological tests. In order to complement the results were analyzed conventional cultivation and isolation of Clostridium estertheticum and Clostridium gasigenes in meat samples. The PCR technique was employed for the purpose of identification of isolates.
Paes, Fernanda Araujo. "AnÃlise de comunidades microbianas de solo de manguezal por T-RFLP e microarranjos de DNA". Universidade Federal do CearÃ, 2008. http://www.teses.ufc.br/tde_busca/arquivo.php?codArquivo=2712.
Pełny tekst źródłaThe mangroves are essistemas important and productive, found along the coastline of tropical and subtropical regions. Important processes such as nutrient cycling, are directly connected to the activity of soil microbial communities. Given its strategic position the mangroves are very impacted the world. In Brazil, important areas such as the Bay of All Saints (BA) are severely affected by the presence of oil. Understanding the adaptation of the microbiota and its local dynamics, before the environmental change is essential to be able to maintain or restore these ecosystems. This thesis investigated the structure and function of microbial communities in a mangrove area of the Bay of All Saints polluted by the oil industry. Two sites were sampled - one located in the refinement of the industry and another, distant location and used as the control. Besides the measured abiotic data, two independent methods of cultivation were used: T-RFLP and DNA microarranjos. The results of both sites in the experiments of T-RFLP showed that the two bacterial communities differ in their structure. For the site sampled in the refinery, a greater number of Otus suggests an environmental stimulus, the bacterial community, a fact associÃvel the high organic matter content of the site. For microarranjos DNA, similar categories of genes were detected in both places, but the specificity of their sequences were distinct. The genes that encode for the remediation organic, were more representative, more than 40% in total in the two sites. Even detecting similar categories, only 4% of the gene sequences were common to the sites. These data show that different bodies are responsible for similar functions at each point. The difference between the sites observed in the experiments can be related with the fact that Site 1 is located in a region heavily affected by oil. Using the ecological data rates of T-RFLP indicated the site 1 as the most diverse, while the results with the DNA microarranjos show the opposite. In conclusion, the structure and function of the local microbiota are affected by the presence of the oil industry. Additional studies may help understand the dynamics of microbial communities, facing the future exposure to the oil and / or derivatives.
Os manguezais sÃo essistemas importantes e produtivos, encontrados ao longo da linha da costa de regiÃes tropicias e subtropicais. Processos importantes como a ciclagem de nutrientes, estÃo diretamente conectados à atividade das comunidades microbianas desses solos. Dada sua posiÃÃo estratÃgica os manguezais sÃo bastante impactados no mundo todo. No Brasil, Ãreas importantes como a da BaÃa de todos os Santos (BA) sÃo severamente afetadas pela presenÃa do petrÃleo. Compreender as adaptaÃÃes da microbiota local e sua dinÃmica, frente Ãs alteraÃÃes ambientais, à essencial para que se consiga manter ou restaurar esses ecossistemas. A presente dissertaÃÃo investigou a estrutura e a funÃÃo de comunidades microbianas em uma Ãrea de manguezal da baÃa de Todos os Santos poluÃda pela indÃstria do petrÃleo. Dois locais foram amostrados - um, situado na Ãrea de refinamento dessa indÃstria e o outro, distante desse local e usado como controle. AlÃm dos dados abiÃticos medidos, dois mÃtodos independentes de cultivo foram utilizados: T-RFLP e microarranjos de DNA. Os resultados de ambos os sÃtios nos experimentos de T-RFLP mostraram que as duas comunidades bacterianas diferem quanto à sua estrutura. Para o sÃtio amostrado na refinaria, um nÃmero maior de OTUs sugere um estÃmulo ambiental, na comunidade bacteriana, fato associÃvel ao alto teor de matÃria orgÃnica desse local. Para os microarranjos de DNA, categorias similares de genes foram detectados, em ambos os locais, mas a especificidade de suas sequÃncias foi distinta. Os genes que codificam para a remediaÃÃo orgÃnica, foram os mais representativos, mais de 40% no total nos dois sÃtios. Mesmo detectando categorias semelhantes, apenas 4% das sequÃncias genÃticas foram comuns aos sÃtios. Estes dados mostram que organismos diferentes sÃo responsÃveis por funÃÃes similares em cada ponto. A diferenÃa entre os locais observadas nos experimentos pode ser relacionada, com o fato do sÃtio 1 situar-se numa regiÃo fortemente afetada por hidrocarbonetos. Utilizando-se Ãndices ecolÃgicos os dados de T-RFLP indicaram o sÃtio 1 como o mais diverso, enquantoo os resultados com os microarranjos de DNA mostram o oposto. Concluindo, a estrutura e funÃÃo da microbiota local sÃo afetadas pela presenÃa da indÃstria do petrÃleo. Estudos adicionais poderÃo ajudar a compreender a dinÃmica dessas comunidades microbianas, frente Ãs futuras exposiÃÃes ao Ãleo e/ou derivados.
Paes, Fernanda Araújo. "Análise de comunidades microbianas de solo de manguezal por T-RFLP e microarranjos de DNA". reponame:Repositório Institucional da UFC, 2008. http://www.repositorio.ufc.br/handle/riufc/4926.
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Os manguezais são ecossistemas importantes e produtivos, encontrados ao longo da linha da costa de regiões tropicais e subtropicias. Processos importantes, como a ciclagem de nutrientes, estão diretamente conectados à atividade das comunidades microbianas desses solos. Dada sua posição estratégica, os manguezais são bastante impactados no mundo todo. No Brasil, áreas importantes como a da Baía de todos os Santos (BA) são severamente afetadas pela presença do petróleo. Compreender as adaptações da microbiota local e sua dinâmica, frente às alterações ambientais, é essencial para que se consiga manter ou restaurar esses ecossistemas. A presente dissertação investigou a estrutura e a função de comunidades microbianas em uma área do manguezal da baía de Todos os Santos, poluída pela indústria do petróleo. Dois locais foram amostrados – um, situado na área de refinamento dessa indústria e o outro, distante desse local e usado como controle. Além dos dados abióticos medidos, dois métodos independentes de cultivo foram utilizados: T-RFLP e microarranjos de DNA. Os resultados de ambos os sítios, nos experimentos de T-RFLP, mostraram que as duas comunidades bacterianas diferem quanto à sua estrutura. Para o sítio amostrado na área da refinaria, um número maior de OTUs sugere um estímulo ambiental, na comunidade bacteriana, fato associável ao alto teor de matéria orgânica desse local. Para os microarranjos de DNA, categorias similares de genes foram detectadas, em ambos os locais, mas a especificidade de suas sequências foi distinta. Os genes que codificam para a remediação orgânica foram os mais representativos, mais de 40% do total nos dois sítios. Mesmo detectando categorias semelhantes, apenas 4% das sequências genéticas foram comuns aos sítios. Estes dados mostram que organismos diferentes são responsáveis por funções similares em cada ponto. A diferença entre os locais, observada nos experimentos, pode ser relacionada com o fato do sítio 1 situar-se numa região fortemente afetada por hidrocarbonetos. Utilizando-se índices ecológicos, os dados de T-RFLP indicaram o sítio 1 como mais diverso, enquanto os resultados dos microarranjos de DNA mostraram o oposto. Concluindo, a estrutura e função da microbiota local são afetadas pela presença da indústria do petróleo. Estudos adicionais poderão ajudar a compreender a dinâmica dessas comunidades microbianas, frente às futuras exposições ao óleo e/ou derivados.
Andrade, Pedro Avelino Maia de. "A composição da comunidade bacteriana do solo como fator determinante na micorrização de cana-de-açúcar por Glomus clarum". Universidade de São Paulo, 2013. http://www.teses.usp.br/teses/disponiveis/11/11140/tde-26062013-143930/.
Pełny tekst źródłaSugarcane is an important Brazilian agricultural system crop and presents currently booming. Nevertheless, land use, and implementation of different management technologies have originated changes in environmental balance, where important microbial interactions occur as essential for plant development. Among the wide diversity of soil microorganisms, the mycorrhizal fungi is highilighted as organisms closely associated with plant roots, helping plants, in any way, to obtain water and nutrients. These fungi however, also interact with other soil organisms, such as for example, bacterial community in these environments. Thus, the present work aimed to study the dynamics of interaction between sugarcane and arbuscularmycorrhizal fungi (AMF) Glomusclarum in soils with different compositions of the bacterial community. The methodology used was \"dilution to extinction\", where serial dilutions (10-1, 10-3, 10-6 and 10-9) of a natural soil were used to inoculate a sterile soil. On this basis, were monitored along a period of 60 days, plant colonization by AMF, and structure of bacterial communities. As a result, we observed a higher colonization of roots of cane sugar for treatments inoculated with lower dilutions of the original community (natural soil and dilutions 10-1 and 10-3), and likewise observed a distinction between these bacterial communities treatments to others. Establishing correlations between microbial groups with observed rates of colonization, it was possible to name, based on the massive sequencing of the region V6 ribosomal gene 16S rDNA, the joint amendment of mycorrhiza with changes in groups of Actinobacteria; Bacteriodetes; Firmicutes, Proteobacteria; Verrucomicrobia and Acidobacteria. In conclusion, this work demonstrates the dependence of an important process, as the AMF, has tosoil bacterial community, and indicates that degraded areas, with lower levels of bacterial diversity, such a process can occur with lower efficiency.
Campana, Felippe Buck. "Monitoramento temporal e espacial de contaminações bacterianas na produção de bioetanol: caracterização molecular por T-RFLP e detecção quantitativa por qPCRde comunidades formadoras de biofilmes". Universidade de São Paulo, 2012. http://www.teses.usp.br/teses/disponiveis/64/64133/tde-25102012-164236/.
Pełny tekst źródłaBacterial contamination by Lactobacillus, Bacillus and Leuconostoc and other lactic acid bacteria is one of the main factors that affects the yield in alcoholic fermentation process. Biofilm formation protects the bacteria community and it is a permanent source of contamination. For characterization of these contaminations in (1) biofilms from centrifuge, tank fermentation, heat exchanger and water pipe and (2) molasses, must, yeast, yeast treated (with H2SO4) and wine, samples were taken at two different periods from fermentation system characterized by high alcohol yields (16%). Restriction enzymes AluI, BstUI, HaeIII, HinfI, MseI and MspI used in T-RFLP analysis were defined by 16S rRNA gene sequences analysis in silico from common contaminants. These enzymes generate high number of unique T-RFs between 30 and 650 bp. DNA from samples were used as template in T-RFLP reactions in order to obtain molecular profiles of microbial communities present at each sample. Shannon diversity index was calculated based on T-RFs numbers. Principal component analysis (PCA) and phylogenetic inference of contaminants were performed based on T-RFs profiles. The main contaminant bacterial taxa were quantified by qPCR using specific primers designed in this study and considering the average of 16S rRNA gene copies previously counted into the genome of each bacterial taxon. Water pipe biofilm showed the highest rate of bacterial diversity in the samples collected in the first sampling period. For the samples collected in the second sampling, the highest rate of bacterial diversity was revealed for molasses and must. PCA suggested that biofilms (but not external sources) are the main contaminants in the studied fermentation process. It is probably due their similarities with the composition of other analyzed communities. Lactobacillus and Bacillus species predominated in first sampling period. Halomonas, Streptococcus, Lactococcus and Pseudomonas were detected in biofilm and liquid samples. They were the main contaminants from biofilm at this time of sampling. In the second sampling period, Bacillus was the most common genera and other lactic acid bacteria such Streptococcus, Staphylococcus and Lactobacillus were also the most frequent contaminants. These results agree with other reported in the literature about conventional fermentation systems. Only the primers designed in this study to amplify the 16S rRNA gene of Burkholderia, Pseudomonas and Weissella showed specificity in tests with bacterial strains. Halomonas sp. was revealed in biofilms from tank fermentation by DNA sequencing using designed primers for genera. Halomonas can produce levan and may consume sucrose available for generation of alcohol. Centrifugal biofilm had the highest amount of bacteria in both sampling periods (1.93E+06 CFU.mg-1 and 2.14E+07 CFU.mg-1, respectively). In liquid samples, yeast had the highest amount of bacteria in both sampling periods (1.03E+08 CFU.ml-1 and 2.96E+06 CFU.ml-1, respectively); it shows significant levels of contaminants. Burkholderia and Pseudomonas were more abundant among biofilm samples of all samplings. Burkholderia was present in high quantities in the majority of liquid samples taken during the first sampling period; Pseudomonas and Weissella equivalently predominated among samples taken during the second sampling period
Części książek na temat "T-RFLP"
Tebbe, Christoph C., Anja B. Dohrmann, Michael Hemkemeyer i Astrid Näther. "Microbial Community Profiling: SSCP and T-RFLP Techniques". W Springer Protocols Handbooks, 101–26. Berlin, Heidelberg: Springer Berlin Heidelberg, 2015. http://dx.doi.org/10.1007/8623_2015_158.
Pełny tekst źródłaHodgetts, Jennifer, i Matt Dickinson. "T-RFLP for Detection and Identification of Phytoplasmas in Plants". W Methods in Molecular Biology, 233–44. Totowa, NJ: Humana Press, 2012. http://dx.doi.org/10.1007/978-1-62703-089-2_20.
Pełny tekst źródłaPark, Min Sik, Aida Barbetti, Toshinao Takenouchi i Paul I. Terasaki. "Analysis of Class II RFLP Patterns and T-Cell Clone Reactions". W Immunobiology of HLA, 921–42. New York, NY: Springer New York, 1989. http://dx.doi.org/10.1007/978-1-4612-3552-1_267.
Pełny tekst źródłaOsborne, Catherine A. "Terminal Restriction Fragment Length Polymorphism (T-RFLP) Profiling of Bacterial 16S rRNA Genes". W Methods in Molecular Biology, 57–69. Totowa, NJ: Humana Press, 2014. http://dx.doi.org/10.1007/978-1-62703-712-9_5.
Pełny tekst źródłaPaïssé, S., M. S. Goñi-Urriza, A. Fahy i R. Duran. "Molecular Profiling of Bacterial Communities via 16S rRNA Gene Based Approaches – Focus T-RFLP". W Handbook of Hydrocarbon and Lipid Microbiology, 4113–25. Berlin, Heidelberg: Springer Berlin Heidelberg, 2010. http://dx.doi.org/10.1007/978-3-540-77587-4_321.
Pełny tekst źródłaKim, Sang-Hoon, i Terence Marsh. "Section 3 update: The Analysis of Microbial Communities with Terminal Restriction Fragment Length Polymorphism (T-RFLP)". W Molecular Microbial Ecology Manual, 2691–710. Dordrecht: Springer Netherlands, 2008. http://dx.doi.org/10.1007/978-1-4020-2177-0_315.
Pełny tekst źródłaSiqueira, José F., Mitsuo Sakamoto i Alexandre S. Rosado. "Microbial Community Profiling Using Terminal Restriction Fragment Length Polymorphism (T-RFLP) and Denaturing Gradient Gel Electrophoresis (DGGE)". W Methods in Molecular Biology, 71–85. Totowa, NJ: Humana Press, 2010. http://dx.doi.org/10.1007/978-1-60761-820-1_6.
Pełny tekst źródłaTiquia, Sonia M. "Using Terminal Restriction Fragment Length Polymorphism (T-RFLP) Analysis to Assess Microbial Community Structure in Compost Systems". W Methods in Molecular Biology, 89–102. Totowa, NJ: Humana Press, 2009. http://dx.doi.org/10.1007/978-1-60761-439-5_6.
Pełny tekst źródłaSiqueira, José F., Mitsuo Sakamoto i Alexandre S. Rosado. "Microbial Community Profiling Using Terminal Restriction Fragment Length Polymorphism (T-RFLP) and Denaturing Gradient Gel Electrophoresis (DGGE)". W Methods in Molecular Biology, 91–104. New York, NY: Springer US, 2022. http://dx.doi.org/10.1007/978-1-0716-2780-8_7.
Pełny tekst źródłaSiqueira, José F., Mitsuo Sakamoto i Alexandre S. Rosado. "Microbial Community Profiling Using Terminal Restriction Fragment Length Polymorphism (T-RFLP) and Denaturing Gradient Gel Electrophoresis (DGGE)". W Methods in Molecular Biology, 139–52. New York, NY: Springer New York, 2016. http://dx.doi.org/10.1007/978-1-4939-6685-1_8.
Pełny tekst źródłaStreszczenia konferencji na temat "T-RFLP"
Mounce, Stephen R., Henriette S. Jensen, Catherine A. Biggs i Joby B. Boxall. "Data mining T-RFLP profiles from urban water system sampling using self-organizing maps". W 2012 8th International Conference on Natural Computation (ICNC). IEEE, 2012. http://dx.doi.org/10.1109/icnc.2012.6234528.
Pełny tekst źródłaDong, Ping, Yujiao Sun, Hongqi Wang, Liding Chen i Hui Zhang. "Notice of Retraction: Study on Riparian Zone Microbial Community Structure of the Wenyu River by T-RFLP Analysis". W 2011 5th International Conference on Bioinformatics and Biomedical Engineering. IEEE, 2011. http://dx.doi.org/10.1109/icbbe.2011.5780057.
Pełny tekst źródłaSharma, Vineeta, Pallavi Singhal, Anoop Kumar, V. G. Ramachandran, Shukla Das i Mausumi Bharadwaj. "Association of TNF-α–rs 281865419 polymorphism with reproductive tract infections in Indian population". W 16th Annual International Conference RGCON. Thieme Medical and Scientific Publishers Private Ltd., 2016. http://dx.doi.org/10.1055/s-0039-1685357.
Pełny tekst źródłaSharma, Vineeta, Pallavi Singhal, Anoop Kumar, V. G. Ramachandran, Shukla Das i Mausumi Bharadwaj. "Association of TNF-α rs-281865419 polymorphism with reproductive tract infections in Indian population". W 16th Annual International Conference RGCON. Thieme Medical and Scientific Publishers Private Ltd., 2016. http://dx.doi.org/10.1055/s-0039-1685270.
Pełny tekst źródłaTáncsics, András, István Szabó, Sándor Szoboszlay, Erzsébet Baka, Károly Márialigeti i Sára Révész. "The role of Beta-Proteobacteria in aromatic hydrocarbon degradation: fingerprinting of 16S rRNA gene and catechol 2,3-dioxygenase gene by T-RFLP in BTEX degradative bacterial communities". W Proceedings of the II International Conference on Environmental, Industrial and Applied Microbiology (BioMicroWorld2007). WORLD SCIENTIFIC, 2009. http://dx.doi.org/10.1142/9789812837554_0138.
Pełny tekst źródłaSakaguchi, Alberto Yoich, Marla Karine Amarante, Carlos Eduardo Coral Oliveira, Fausto Celso Trigo i Maria Angelica Ehara Watanabe. "INFLUÊNCIA DO POLIMORFISMO GENÉTICO DO RECEPTOR II DO TGF BETA NA APRESENTAÇÃO CLÍNICA DE PACIENTES COM LEUCEMIA LINFOIDE AGUDA INFANTOJUVENIL NA POPULAÇÃO BRASILEIRA". W I Congresso Brasileiro de Hematologia Clínico-laboratorial On-line. Revista Multidisciplinar em Saúde, 2021. http://dx.doi.org/10.51161/rems/625.
Pełny tekst źródłaBernaedi, F., V. Bertagnolo, S. Bartolai, L. Rossi, F. Panicucci i F. Conconi. "A POINT MUTATION AND A GENE DELETION OF FVIII GENE IN SEVERE HAEMOPHILIA". W XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1644047.
Pełny tekst źródłaRaporty organizacyjne na temat "T-RFLP"
Michel Jr., Frederick C., Harry A. J. Hoitink, Yitzhak Hadar i Dror Minz. Microbial Communities Active in Soil-Induced Systemic Plant Disease Resistance. United States Department of Agriculture, styczeń 2005. http://dx.doi.org/10.32747/2005.7586476.bard.
Pełny tekst źródłaDubcovsky, Jorge, Tzion Fahima i Ann Blechl. Positional cloning of a gene responsible for high grain protein content in tetraploid wheat. United States Department of Agriculture, wrzesień 2003. http://dx.doi.org/10.32747/2003.7695875.bard.
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