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Schrobback, Peggy. "Economic analyses of Australia's Sydney rock oyster industry". Thesis, Queensland University of Technology, 2015. https://eprints.qut.edu.au/83730/1/Peggy_Schrobback_Thesis.pdf.
Pełny tekst źródłaDove, Michael Colin. "Effects of estuarine acidification on survival and growth of the Sydney rock oyster Saccostrea glomerata". Connect to this title online, 2003. http://www.library.unsw.edu.au/~thesis/adt-NUN/public/adt-NUN20050125.110005/index.html.
Pełny tekst źródłaRubio, Ana M. "Environmental influences on the sustainable production of the Sydney rock oyster Saccostrea glomerata : a study in two Southeastern Australian estuaries /". View thesis entry in Australian Digital Theses Program, 2007. http://thesis.anu.edu.au/public/adt-ANU20080618.091057/index.html.
Pełny tekst źródłaArumugaswamy, Ramakrishnaswamy, Hawkesbury Agricultural College i Faculty of Food and Environmental Sciences. "Studies on the presence and survival of campylobacter species in the Sydney rock oyster (Crassostrea commercialia)". THESIS_FES_XXX_Arumugaswamy_R.xml, 1985. http://handle.uws.edu.au:8081/1959.7/412.
Pełny tekst źródłaMaster of Science (Hons)
Dove, Michael Colin Geography Program UNSW. "Effects of estuarine acidification on survival and growth of the Sydney rock oyster Saccostrea glomerata". Awarded by:University of New South Wales. Geography Program, 2003. http://handle.unsw.edu.au/1959.4/20485.
Pełny tekst źródłaArumugaswamy, Ramakrishnaswamy. "Studies on the presence and survival of campylobacter species in the Sydney rock oyster (Crassostrea commercialia)". Thesis, View thesis, 1985. http://handle.uws.edu.au:8081/1959.7/412.
Pełny tekst źródłaArumugaswamy, Ramakrishnaswamy. "Studies on the presence and survival of campylobacter species in the Sydney rock oyster (Crassostrea commercialia) /". View thesis, 1985. http://library.uws.edu.au/adt-NUWS/public/adt-NUWS20031205.122556/index.html.
Pełny tekst źródłaNguyen, Dien. "Assessing genetic diversity in cultured aquatic species : the Sydney Rock Oyster (Saccostrea glomerata) stock improvement program as a model". Thesis, Queensland University of Technology, 2009. https://eprints.qut.edu.au/41460/1/Dien_Nguyen_Thesis.pdf.
Pełny tekst źródłaRubio, Zuazo Ana Maria, i anarubio zuazo@gmail com. "Environmental influences on the sustainable production of the Sydney rock oyster Saccostrea glomerata : a study in two southeastern Australian estuaries". The Australian National University. Centre for Resource and Environmental Studies, 2008. http://thesis.anu.edu.au./public/adt-ANU20080618.091057.
Pełny tekst źródłaHand, RE. "Commercialisation of Triploid Sydney Rock Oysters Saccostrea Glomerata in New South Wales growth survival and meat condition". Thesis, Honours thesis, University of Tasmania, 2002. https://eprints.utas.edu.au/7571/1/Front_Matter_Hand.pdf.
Pełny tekst źródłaEnglish, Louise (Louise Jasmins). "Genetic diversity in oysters". Thesis, 2001. https://eprints.utas.edu.au/19736/1/whole_EnglishLouiseJasmins2001_thesis.pdf.
Pełny tekst źródłaHand, Rosalind Elizabeth. "Commercialisation of triploid Sydney rock oysters, Saccostrea Glomerata, in New South Wales : growth, survival and meat condition". Thesis, 2002. https://eprints.utas.edu.au/19764/1/whole_HandRosalindElizabeth2002_thesis.pdf.
Pełny tekst źródłaSarwer, Md Golam. "Impact of ecological changes on Sydney rock oyster genetics and microbiomes". Thesis, 2020. http://hdl.handle.net/1959.7/uws:67286.
Pełny tekst źródłaPearson, Benjamin. "Bioaccumulation of heavy metals in the Sydney rock oyster, saccostrea commercialis (Iredale & Roughley)". 1993. http://hdl.handle.net/2100/629.
Pełny tekst źródłaThe suitability of the Sydney rock oyster (Saccostrea commercialis) as a monitor of heavy metal contamination in estuarine and coastal waters was assessed. Case study I examined the influence of body size (ie: soft tissue dry weight) on the tissue concentrations of Cu, Zn, and Fe. Sampling was replicated both spatially and temporally in order to determine whether the size-metal relationship for these elements varied with geographical location (ie: differing levels of environmental contamination) or season. All oysters were collected from 'wild' populations in the HawkesburyfNepean estuary. Least-squares regression analysis indicated that Cu concentrations were independent of body size at all times. Analysis of covariance (ANCOVA) showed that this relationship did not change with either geographical location or season. Size-metal relalionships for Zn indicated that concentrations for this element varied from being independent of size to being size-dependent with proportionally greater concentrations present in the larger individuals. Yet lests for homogeneily of slopes (ANCOVA) showed that no differences in regression coefficients occurred with geographical location or season. Iron concentrations were size-dependent with proportionally greater concentrations in the smaller individuals. ANCOVA revealed that homogeneity of slopes with geographical location occurred only on two of the four sampling occasions. Further examination of data revealed that the size·range of the individuals sampled may be important in determining size-metal relationship for iron. Case Study II examined the concentrations of Cu and Zn in S. commercialis deployed into the Georges River/Botany Bay and the Port Stephens estuaries. The influence of tissue assimilation/loss on metal concenlralions was also examined. Organisms of a homogenous genetic stock, of a similar size and age, and transplanted to a similar lidal height were utilized. As all organisms were of a similar size and age at the time of their deployment any differences in size during sampling were attributed to growth. Results indicated that tissue loss (ie: 'degrowth') not accompanied by a similar loss of metal resulted in an increase in metal concentrations. The assimilation of new tissue at a greater rate than metal accumulation (ie: 'dilution') resulted in decreased metal concentrations. From these relationships it was apparent that measures must be taken to reduce, account for, or eliminate the effects of growth in bivalve monitoring programs. Unless this problem is addressed, metal concentration values in S. commercialis are likely to give an inaccurate estimation of the level of environmenlal contamination and confound attempts to isolate pollution point sources.
Holliday, J. E. "Nursery culture of Sydney rock oysters, Saccostrea commercialis (Iredale & Roughley, 1933) and Pacific oysters, Crassostrea gigas (Thunberg 1793)". Thesis, 1995. https://eprints.utas.edu.au/20333/1/whole_HollidayJE1995_thesis.pdf.
Pełny tekst źródłaYingprasertchai, Thanvapon Senee. "Influence of metal exposure history on metal tolerance in the Sydney rock oyster (Saccostrea glomerata)". Thesis, 2016. http://hdl.handle.net/1959.13/1312288.
Pełny tekst źródłaTolerance to heavy metal pollution has been described in many living organisms exposed to heavy metals at sublethal levels. Organisms living in metal contaminated sites have to develop physiological processes to respond to metal exposure to ensure survival and reproduction. The organisms may respond to this challenge through physiological acclimation or adaptation (the selection of resistant genotypes). This thesis was focused on the influence of prior metal exposure history on metal tolerance in Sydney rock oyster, Saccostrea glomerata in NSW, Australia. The thesis explored both physiological acclimation and possible adaptation to metal stress. To determine the level of metal contamination among estuaries in NSW and indicate the most relevant metals for further experimental studies, multivariate statistical techniques such as principle component analysis (PCA) and factor analysis (FA), and ecological risk indices including contamination factor (Cf), degree of contamination (Cd) and pollution loading index (PLI) were employed. Using accumulated metals in tissues of the oysters growing wild and farmed in NSW, these analyses identified locations both low and elevated in metals and identified metals most responsible for driving differences among locations.. The results indicated that most of polluted estuaries were located in the Central region of NSW, having impacts from large urban and industrial conurbation, while the unpolluted estuaries were located in the Northern / Southern region within the major regional waterways with low urban activity. The most elevated metals among NSW estuaries were cadmium, copper, lead and zinc. To investigate possible adaptation, the influence of metal exposure history on metal tolerance in the Sydney rock oyster (Saccostrea glomerata) offspring was investigated. The oysters were sampled from ten NSW estuaries such as Clyde River, Hastings River, Hunter River, Manning River, Nambucca, North Haven, Port Stephens, Port Kembla, Swansea Channels, and Wallis Lake which have a gradient in metal exposure history based on the results of multivariate analysis (PCA/FA) and ecological indices (Cf, Cd and PLI) of metals accumulated to adult oysters. Embryos were prepared under laboratory conditions by fertilizing sperm from ten males and oocytes from ten females within estuary using a strip spawning technique. The embryos were then exposed to sublethal concentrations of 2.5-40 μg/l, copper and 7.5-120 μg/l zinc for 48 h. The median effective concentration (EC50) was determined as the metal concentration that caused 50% abnormal D-veliger larvae development.. The EC50 values for copper were not significantly different among estuaries. Interestingly, a strong positive correlation between EC50 and tissue metal concentration was found for zinc (R² = 0.835, p<0.000), but not for copper. Locations with higher environmental Zn exposures had more tolerant offspring. Such a finding suggests adaptation to Zn exposure (the selection of resistant genotypes) or a physiological acclimation effect mediated via maternal transfer. The full-length genomic sequence of S. glomerata, metallothionein (sgMT) was cloned and characterized. The sgMT sequence was composed of two metal responsive elements (MRE), one TATA box, one activator protein-1 (AP-1), three coding exons (28, 117, 80 bp), and two introns (115, 393 bp). The coding exons encoding a protein of 74 amino acids, with 9 cysteine motifs (Cys-X-Cys), cysteine-rich (28%), high lysine content (13.5%) and no aromatic amino acid residues. The phylogenetic analysis of sgMT protein sequence revealed that the sgMT was classified into the MT isoform I. Sequencing of full length of MT mRNA and MT genomic DNA allowed the identification of an intron-exon boundary and the development of the quantitative real time polymerase chain reaction (qRT-PCR). The qRT-PCR revealed that tissue-specific MT gene expression in S. glomerata was expressed highest in the digestive gland; significantly higher than gills, mantle, adductor muscle and gonad. The digestive gland was selected as a target tissue for a further examination of S. glomerata MT gene expression. Physiological acclimation to metals was also investigated by comparing adult oysters with a past history of metal exposure to oysters with no past history of metal exposure (both from the same gene pool) and examining their responses to subsequent metal challenges. Specifically, the experiment involved examining the influence of prior metal exposure history in wild Sydney rock oyster (Saccostrea glomerata) on metal accumulation, MT mRNA expression, glutathione peroxidase (GPx) and superoxide dismutase (SOD) upon subsequent cadmium, copper and zinc exposure. Oysters were sampled from seven locations in Hunter River such as Fullerton Cove, Kooragang Dykes, Windmill, South Arm Bridge, Fern bay, Fullerton St Stockton and Stockton Bridge. The results of multivariate analysis (PCA/FA) and ecological indices (Cf and Cd) of metal accumulation in the oyster tissue from seven locations elucidated that Windmill was the most contaminated with metals, while Fern Bay was the lowest. Copper and zinc were the most elevated metals with 3.71 and 2.51 times higher than NSW background, thus these metals were selected as metal candidates for metal exposure experiment. Cadmium was included as a positive control. Windmill(high past metal exposure history) and Fern Bay( negligible past metal exposure history)oysters were exposed to 20 and 200 μg/l of cadmium, 50 and 500 μg/l of copper and 200 and 2000 μg/l of zinc for 14 days. After exposure, MT mRNA expression, GPx and SOD activities were measured in digestive glands. The two-way ANOVA results revealed that Windmill oysters activated MT mRNA expression significantly higher than Fern bay oysters after exposure to 200 μg/l Cd, F(2, 22) = 10.35, p = 0.0007 and 2000 μg/l Zn, F(2, 21) = 24.28, p < 0.0001. The GPx activity was not significantly different between those oysters. The SOD activity showed significant interaction effects for copper with F(2, 21) = 10.91, p = 0.0006, indicating that Windmill oysters produced SOD activity significantly higher than Fern bay oysters after 50 μg/l Cu exposure. Significant interaction was also found for zinc with F(2, 24) = 7.27, p = 0.0034, where Fern bay oysters produced SOD higher than Windmill oysters after 200 μg/l Zn exposure, while Windmill oysters produced SOD higher than Fern bay oysters after 2000 μg/l Zn exposure. Thus prior exposure to metals can result in an upregulated compensatory response upon subsequent exposure to metals indicative of acclimation. Both acclimation, and potentially adaptation, are mechanisms responsible for the observed tolerance to metals in the Sydney Rock Oyster.
Krassoi, Frederick Rudolf. "Population ecology of the Sydney rock oyster saccostrea commercialis and the pacific oyster crassostrea gigas in a New South Wales estuary". 2001. http://hdl.handle.net/2100/1107.
Pełny tekst źródłaThe study of place was often divided between the spatial interests of geographers and local historians intent on constructing heroic lineages. In the period of accelerated globalization however, discrete discourses on time and space are no longer tenable. Histories of place engage the transdisciplinary approach of recent scholarship in understanding the complexities and fluidity of the world in which we live. Places are constructed out of the enmeshing of the material, social and cultural. The reasons why people migrate both within and to particular places are also critical to the ongoing perceptions of that place, and the dynamics by which local communities operate within global networks. This thesis is an historical study of a recent sewage ocean outfall dispute between residents and the local council at Emerald Beach, in the Coffs Harbour region of New South Wales' Mid-North Coast. Alongside documentary sources, it uses oral testimony to examine the factors that contributed to people's understanding of their place, and the processes that resulted in the public contestation over that place. It argues that the positions taken in the sewage dispute cannot simply be perceived as a function of individual residents' responses within a bounded local context, but were a result of the complex processes of internal migration to the region since colonisation, and especially since the 1970s, that brought competing visions for the same place. In exploring the historical traces of the dispute, the thesis examines the first wave of non-Aboriginal migration to the coastal hinterland before turning attention to the second intensive wave of migration in the postwar period. Attention shifted away from the hinterland to the coast, and the chapters examine competing uses for the coast as local born residents, tourists and the influx of new settlers from the 1970s brought diverse dreams for the warm North Coast. In particular, the sewage conflict that grew into the direct-action protests at Emerald Beach provides clear insights into the flows of migration and settlement that led to the particular mix of people who fought for their divergent conceptions of place as critical to their lifestyle and residency. Without examining historical representations of places and events, conflict situations such as the sewage dispute at Emerald Beach cannot be fully illuminated. By demonstrating the force of internal migration on perceptions of, and contestation within place, this thesis provides one framework from which other places might be investigated.
Ogburn, Damian M. "The NSW Oyster Industry: A Risk Indicator of Sustainable Coastal Policy and Practice". Phd thesis, 2011. http://hdl.handle.net/1885/8558.
Pełny tekst źródłaNguyen, Thi Hong Tham. "Molecular cloning and characterisation of two insulin-like growth factor binding protein genes (sgIGFBP-5 AND sgIGFBP-7) from the Sydney rock oyster Saccostrea glomerata". Thesis, 2020. http://hdl.handle.net/1959.13/1411170.
Pełny tekst źródłaInsulin-like growth factors (IGFs) play crucial roles in regulating growth, development and metabolism in animals. IGFs are complexed with specific binding proteins known as IGF binding proteins (IGFBPs), which modulate, either stimulate or inhibit, the action of IGFs. Most of the current literature on oyster biology focuses on reproductive cycle and growth patterns as well as their relationship with their ambient environmental conditions. Compared with vertebrates, little is known about the biology of IGFBPs and the molecular mechanisms involved in the regulation of growth in invertebrates, particularly molluscs. To fill this knowledge gap, both the cDNA and genomic DNA sequences of the IGFBP-5 and IGFBP-7 homologues were cloned from the Sydney rock oyster (SRO), Saccostrea glomerata. The spatial and temporal mRNA expression patterns, single nucleotide polymorphisms (SNPs) and their consequent haplotypes of these two genes as well as their transcriptional responses to fasting and hypoxia were investigated to decipher their potential roles in the regulation of the SRO growth. The full-length cDNA and gDNA sequences of the S. glomerata IGFBP-5 and IGFBP-7 genes (designated sgIGFBP-5 and sgIGFBP-7, respectively) were cloned and characterised for the first time. For sgIGFBP-5, the ORF consists of 357 bp, encoding a polypeptide of 118 amino acid residues. The deduced amino acid sequence of sgIGFBP-5 contained conserved N- and C-terminal regions, multiple cysteine residues and two conserved motifs (GCGCCXXC and CWCV). There are multiple putative regulatory DNA elements in the promoter region of sgIGBFP-5 which might interact with specific transcription factors to drive gene transcription, including an ERE half site, a SP1 site, an AP-1 site and a HRE. The genomic DNA of sgIGBFP-5 consists of 3 exons and 2 introns. For sgIGFBP-7, its ORF comprises of 780 bp, encoding a polypeptide of 259 amino acid residues. The sgIGFBP-7 protein consists of three conserved domains, including the IGFBP N-terminal domain, the Kazal-type serine protease inhibitor domain and the immunoglobin (Ig) domain, as well as one conserved motif “xCGCCxxC”. The promoter region of sgIGFBP-7 comprises of multiple putative regulatory DNA elements, including an ERE half site, an EGR1 binding site, a NF-κB cells binding site and a HRE core sequence. Phylogenetic analysis showed that the deduced proteins of sgIGFBP-5 and sgIGFBP-7 are clustered with their corresponding homologues from other molluscan species, supporting the proper nomenclature of the SRO genes. All of the observations above suggest that sgIGBFP-5 and sgIGBFP-7 is authentic IGFBP homologues and their genomic and deduced protein structures are conserved evolutionarily. Expression of sgIGFBP-5 and sgIGFBP-7 mRNA was detected in all the tested tissues in both juvenile and adult oysters. Notably, contrasting differential expression profiles of sgIGFBP-5 and sgIGFBP-7 mRNAs between the adult slow-growing and fast-growing oysters were observed in certain tissue types, implying that IGFBPs may play potential roles in regulating the growth of SRO. DNA sequencing of 100 oysters from each of the slow and fast-growing lines revealed the presence of 183 and 76 SNPs in the genomic regions of sgIGFBP-5 and sgIGFBP-7, respectively. Among these, 7 SNPs in sgIGFBP-5 and 16 SNPs in sgIGFBP-7 are significantly different in genotype/allele frequencies between the two oyster lines. The association of the SNPs identified in sgIGFBP-5 and sgIGFBP-7 with growth traits was examined among the oyster individuals grouped according to their genotypes constituted by each SNP. For sgIGFBP-5, significant differences were found in shell length among the genotypes constituted by six SNPs and in shell height among the genotypes constituted by all seven SNPs. For sgIGBFP-7, significant differences in shell length, shell height and whole oyster weight were found among the genotypes constituted by the majority of the identified SNPs. The association of sgIGFBP-5 and sgIGFBP-7 haplotypes with growth traits was also investigated. Statistically significant differences among the haplotypes were observed with respect to shell length and shell height for sgIGFBP-5 while no significant difference among haplotypes with respect to any of the tested growth traits for sgIGFBP-7 was found. These results suggest that the SNPs/haplotypes in sgIGFBP-5 and sgIGFBP-7 may have a significant association with the growth traits in S. glomerata and hence they may provide the basis for developing marker-assisted selection strategies to improve the oyster’s growth performance in the future. An in silico protein-protein interaction analysis was performed to identify the strengths of the potential binding between the deduced protein of each nonsynonymous haplotype groups and their potential ligands. The results revealed that the orders of binding strength of the tested ligands with each of the sgIGFBP-5 and sgIGFBP-7 haplotype groups were different. This finding suggests that sgIGFBP-5 and sgIGFBP-7 may have their own binding preferences for different cellular ligands, implying that the non-synonymous SNPs presented in the haplotype groups may give rise to critical changes in the 3D structure of the encoded IGFBP proteins, which in turn alter IGFBP-ligand interaction. A short-term starvation experiment and investigation of the regulatory effect of hypoxia on mRNA expression of sgIGFBP-5 and sgIGFBP-7 were conducted. For the fasting experiment, the sgIGFBP-5 and sgIGFBP-7 expression levels in both digestive gland and mantle tissue in fasting oysters increased compared to controls at the time points of Days 3, 5 and 7. This lead to the postulation that catabolic activity was increased and IGFBPs in the starved oysters are released into the circulation to bind to IGFs, thereby restricting IGFs from binding to their cognate receptors and hence slow down the oysters’ growth. The study also pointed out that re-feeding (2 days) of fasting oysters restored the basal expression of the IGFBP mRNA. This may suggest that the downregulation of these genes could in turn release the availability of IGFs for IGF-receptor binding or growth. However, this was the short-term starvation experiment, and the changes in growth traits of S. glomerata were not measured. Thus, it remains premature to conclude the roles of the sgIGFBPs in growth regulation. For the hypoxic experiment, no significant change in HIF-1 and IGFBP mRNA expression were detected in any of the tested oyster tissues after 5 days of air exposure. These observations do not agree with a hypothesis that HIF-1 expression is increased under hypoxic conditions and the increased HIF-1 availability can in turn activate IGFBP gene transcription via binding to the HRE(s) located in the IGFBP gene promoter. Overall, the findings from this research provide information on the structures and potential functions of IGFBPs in relation to growth and stress effects. In the future, from the SNPs and/or haplotypes in sgIGFBP-5 and sgIGFBP-7, identification of growth associated genetic markers will contribute to improving the genetic stock, productivity, sustainability and profitability of the SRO (and potentially other molluscan species).
Rubio, Zuazo Ana Maria. "Environmental influences on the sustainable production of the Sydney rock oyster Saccostrea glomerata : a study in two southeastern Australian estuaries". Phd thesis, 2007. http://hdl.handle.net/1885/49349.
Pełny tekst źródłaTran, Thi Kim Anh. "Investigation of the molecular mechanisms underlying estrogen-mediated induction of vitellogenin gene expression in the Sydney rock oyster, Saccostrea glomerata". Thesis, 2017. http://hdl.handle.net/1959.13/1343169.
Pełny tekst źródłaEnvironmental estrogens are known to interfere with normal development and reproduction in a wide range of marine invertebrates. Previous studies have developed an oyster biomonitor to indicate the presence of estrogenic compounds in marine environments through exploiting the induction of the egg yolk protein precursor vitellogenin (Vtg). Despite this advance, the mechanism through which estrogens exert their action on Vtg gene expression, in particular the functional role of the estrogen receptor (ER), is currently unknown. In an attempt to fill this knowledge gap, the present research aims to isolate the genes encoding Vtg (sgVtg) and ER (sgER) from the Sydney rock oyster Saccostrea glomerata and investigate different potential mechanisms contributing to estrogen mediated induction of sgVtg. Our results indicated that the deduced protein of sgVtg is substantially longer than those of the other oyster Vtgs reported so far and contains all the conserved domains as found in other marine molluscs. The sgVtg promoter contains multiple putative half-EREs which are closely spaced, implying that they may function as an estrogen response unit (ERU) to interact with ER. In line with the potential involvement of ER in sgVtg regulation, the induction of sgVtg mRNA expression in ovarian explants was shown to be abolished by the ER antagonist ICI 182, 780. Considering that the vertebrate-like ER so far reported in molluscs lacks estrogen-binding ability, this finding supports the requirement of a novel estrogen-binding receptor for gene activation. The sgER cDNA is predicted to encode a 477-amino acid protein, which contains a DNA binding domain (DBD) and a ligand binding domain (LBD) conserved among vertebrate and invertebrate ERs. Comparison of the sgER LBD sequence with those of other ligand-dependent ERs indicated that the sgER LBD is degenerate at several conserved residues critical for ligand binding and receptor activation. Its inability to bind estrogens was then confirmed by a ligand binding assay using fluorescent-labelled E2 and purified sgER protein. The 5′-flanking region of sgER contains three putative ½EREs and several other putative elements for ER-interacting transcription factors, suggesting potential autoregulation of sgER expression. sgER mRNA is ubiquitously expressed in various tissues, with the highest expression level observed in the ovary where sgVtg is highly expressed. Functional analyses, including luciferase gene reporter assays, point mutation of ½ EREs and an electrophoretic mobility shift assay (EMSA), confirmed that sgER binds and activates the sgVtg promoter through ½EREs. In addition, sgER mRNA was significantly upregulated following in vitro and in vivo exposure to E2 and the enhancing effect of E2 on sgER expression was abolished by co-treatment with the specific ER antagonist ICI 182, 780 in vitro. These findings support the presence of a novel estrogen-binding receptor in S. glomerata. To elucidate whether estrogens modulate sgVtg and sgER expression at the epigenetic level, we assessed the DNA methylation levels of a 5’ intragenic CpG island in sgVtg and a promoter CpG island in sgER in ovaries after E2 exposure in vivo. Bisulfite sequencing revealed that both of these CpG islands are hypomethylated in both control and E2-treated oysters. Neither significant differential DNA methylation nor correlation between DNA methylation and mRNA levels was observed for either sgVtg or sgER. Overall, the findings from this research provides new molecular insights into how environmental estrogens regulate Vtg expression in marine molluscs and lays the foundation for further research into the mechanism of action of estrogenic compounds on molluscan vitellogenesis.
Parker, Laura M. "Climate change : the synergisitic impacts of elevated pCO2 and temperature on the early development of oysters". Thesis, 2010. http://handle.uws.edu.au:8081/1959.7/507161.
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