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Carpentier, Mathilde. "Méthodes de détection des similarités structurales : caractérisation des motifs conservés dans les familles de structures pour l' annotation des génomes". Paris 6, 2005. http://www.theses.fr/2005PA066571.
Pełny tekst źródłaMahajan, Swapnil. "Applications d'un alphabet structural pour l'analyse, la prédiction et la reconnaissance des repliements des protéines". Thesis, La Réunion, 2013. http://www.theses.fr/2013LARE0032.
Pełny tekst źródłaAnalysis of protein structures using structural alphabets has provided new insights into protein function and evolution. We have used a structural alphabet called proteins blocks (PBs) which efficiently approximates protein backbone and allows abstraction of 3D protein structures into 1D PB sequences. This thesis describes applications of PBs for protein structure analysis, prediction and fold recognition. First, PBs were used to provide a refined view of structurally variable regions (SVRs) in homologous proteins in terms of conformationally similar and dissimilar SVRs in which were compiled a database of structural alignments (DoSA). We also show that the inherent conformational variations in loop regions are not correlated to corresponding conformational differences in their homologues. Second, to further analyze sequence-structure relationships in terms of PBs and other structural features, we have set up a database of pentapeptides derived from protein structures. This served as a basis for the knowledge-based prediction of local protein structure in terms of PB sequences (PB-kPRED) and of local structure plasticity (PB-SVindex). We demonstrate the successful applications of PB-kPRED for fold recognition and explored possible identification of structural and functional hotspots in proteins using PB-SVindex. Finally, an algorithm for fold recognition using a structural alphabet (FoRSA) based on calculation of conditional probability of sequence-structure compatibility was developed. This new threading method has been successfully benchmarked on a test dataset from CASP10 targets. We further demonstrate the application of FoRSA for fast structural annotations of genomes
Keyhani, Ali. "A Study On The Predictive Optimal Active Control Of Civil Engineering Structures". Thesis, Indian Institute of Science, 2000. https://etd.iisc.ac.in/handle/2005/223.
Pełny tekst źródłaKeyhani, Ali. "A Study On The Predictive Optimal Active Control Of Civil Engineering Structures". Thesis, Indian Institute of Science, 2000. http://hdl.handle.net/2005/223.
Pełny tekst źródłaPeters, David W. "Design of diffractive optical elements through low-dimensional optimization". Diss., Georgia Institute of Technology, 2001. http://hdl.handle.net/1853/54614.
Pełny tekst źródłaEdrees, Tarek. "Structural Identification of Civil Engineering Structures". Licentiate thesis, Luleå tekniska universitet, Byggkonstruktion och -produktion, 2014. http://urn.kb.se/resolve?urn=urn:nbn:se:ltu:diva-26719.
Pełny tekst źródłaGodkänd; 2014; 20141023 (taredr); Nedanstående person kommer att hålla licentiatseminarium för avläggande av teknologie licentiatexamen. Namn: Tarek Edrees Saaed Ämne: Konstruktionsteknik/Structural Engineering Uppsats: Structural Identification of Civil Engineering Structures Examinator: Professor Jan-Erik Jonasson, Institutionen för samhällsbyggnad och naturresurser, Luleå tekniska universitet Diskutant: Forskare Andreas Andersson, Brobyggnad inklusive Stålbyggnad, Kungliga Tekniska Högskolan Tid: Torsdag den 20 november 2014 kl 10:00 Plats: F1031, Luleå tekniska universitet
BABAEI, IMAN. "Structural Testing of Composite Crash Structures". Doctoral thesis, Politecnico di Torino, 2021. http://hdl.handle.net/11583/2910072.
Pełny tekst źródłaRasmussen, Kim J. R. "Stability of thin-walled structural members and systems". Thesis, The University of Sydney, 2017. http://hdl.handle.net/2123/18194.
Pełny tekst źródłaIrakarama, Modeste. "Towards Reducing Structural Interpretation Uncertainties Using Seismic Data". Electronic Thesis or Diss., Université de Lorraine, 2019. http://www.theses.fr/2019LORR0060.
Pełny tekst źródłaSubsurface structural models are routinely used for resource estimation, numerical simulations, and risk management; it is therefore important that subsurface models represent the geometry of geological objects accurately. The first step in building a subsurface model is usually to interpret structural features, such as faults and horizons, from a seismic image; the identified structural features are then used to build a subsurface model using interpolation methods. Subsurface models built this way therefore inherit interpretation uncertainties since a single seismic image often supports multiple structural interpretations. In this manuscript, I study the problem of reducing interpretation uncertainties using seismic data. In particular, I study the problem of using seismic data to determine which structural models are more likely than others in an ensemble of geologically plausible structural models. I refer to this problem as "appraising structural models using seismic data". I introduce and formalize the problem of appraising structural interpretations using seismic data. I propose to solve the problem by generating synthetic data for each structural interpretation and then to compute misfit values for each interpretation; this allows us to rank the different structural interpretations. The main challenge of appraising structural models using seismic data is to propose appropriate data misfit functions. I derive a set of conditions that have to be satisfied by the data misfit function for a successful appraisal of structural models. I argue that since it is not possible to satisfy these conditions using vertical seismic profile (VSP) data, it is not possible to appraise structural interpretations using VSP data in the most general case. The conditions imposed on the data misfit function can in principle be satisfied for surface seismic data. In practice, however, it remains a challenge to propose and compute data misfit functions that satisfy those conditions. I conclude the manuscript by highlighting practical issues of appraising structural interpretations using surface seismic data. I propose a general data misfit function that is made of two main components: (1) a residual operator that computes data residuals, and (2) a projection operator that projects the data residuals from the data-space into the image-domain. This misfit function is therefore localized in space, as it outputs data misfit values in the image-domain. However, I am still unable to propose a practical implementation of this misfit function that satisfies the conditions imposed for a successful appraisal of structural interpretations; this is a subject for further research
Irakarama, Modeste. "Towards Reducing Structural Interpretation Uncertainties Using Seismic Data". Thesis, Université de Lorraine, 2019. http://www.theses.fr/2019LORR0060/document.
Pełny tekst źródłaSubsurface structural models are routinely used for resource estimation, numerical simulations, and risk management; it is therefore important that subsurface models represent the geometry of geological objects accurately. The first step in building a subsurface model is usually to interpret structural features, such as faults and horizons, from a seismic image; the identified structural features are then used to build a subsurface model using interpolation methods. Subsurface models built this way therefore inherit interpretation uncertainties since a single seismic image often supports multiple structural interpretations. In this manuscript, I study the problem of reducing interpretation uncertainties using seismic data. In particular, I study the problem of using seismic data to determine which structural models are more likely than others in an ensemble of geologically plausible structural models. I refer to this problem as "appraising structural models using seismic data". I introduce and formalize the problem of appraising structural interpretations using seismic data. I propose to solve the problem by generating synthetic data for each structural interpretation and then to compute misfit values for each interpretation; this allows us to rank the different structural interpretations. The main challenge of appraising structural models using seismic data is to propose appropriate data misfit functions. I derive a set of conditions that have to be satisfied by the data misfit function for a successful appraisal of structural models. I argue that since it is not possible to satisfy these conditions using vertical seismic profile (VSP) data, it is not possible to appraise structural interpretations using VSP data in the most general case. The conditions imposed on the data misfit function can in principle be satisfied for surface seismic data. In practice, however, it remains a challenge to propose and compute data misfit functions that satisfy those conditions. I conclude the manuscript by highlighting practical issues of appraising structural interpretations using surface seismic data. I propose a general data misfit function that is made of two main components: (1) a residual operator that computes data residuals, and (2) a projection operator that projects the data residuals from the data-space into the image-domain. This misfit function is therefore localized in space, as it outputs data misfit values in the image-domain. However, I am still unable to propose a practical implementation of this misfit function that satisfies the conditions imposed for a successful appraisal of structural interpretations; this is a subject for further research
Denli, Huseyin. "Structural-acoustic optimization of composite sandwich structures". Access to citation, abstract and download form provided by ProQuest Information and Learning Company; downloadable PDF file, 168 p, 2007. http://proquest.umi.com/pqdlink?did=1251904511&Fmt=7&clientId=79356&RQT=309&VName=PQD.
Pełny tekst źródłaNaseer, Abeer. "Structural studies of higher order DNA structures". Thesis, University of Reading, 2009. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.515807.
Pełny tekst źródłaYahathugoda, B. P. S. Dilruk. "Structural mechanics of plain knitted elastomeric structures". Thesis, University of Manchester, 2005. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.607626.
Pełny tekst źródłaZHANG, Jingyao. "STRUCTURAL MORPHOLOGY AND STABILITY OF TENSEGRITY STRUCTURES". 京都大学 (Kyoto University), 2007. http://hdl.handle.net/2433/49132.
Pełny tekst źródłaKyoto University (京都大学)
0048
新制・課程博士
博士(工学)
甲第13385号
工博第2856号
新制||工||1420(附属図書館)
25541
UT51-2007-Q786
京都大学大学院工学研究科建築学専攻
(主査)教授 加藤 直樹, 教授 上谷 宏二, 准教授 大﨑 純
学位規則第4条第1項該当
Stuani, Lucille. "Nouveaux aspects du métabolisme d'acinetobacter baylyi ADP1 : une approche métabolomique". Thesis, Evry-Val d'Essonne, 2014. http://www.theses.fr/2014EVRY0048/document.
Pełny tekst źródłaWhile incomplete, the knowledge of metabolism remains a priority for both basic and applied research. With approximately 40 % of genes of prokaryotic genomes with no accurate function, a number of metabolic pathways remain incomplete, which impedes the accurate prediction of the metabolism of a cell.Recent progress and breakthroughs in the understanding of the Omics Cascade that includes genomics, transcriptomics, proteomics and metabolomics play an important role in the knowledge of metabolism. As intermediaries of biochemical reactions, metabolites connect the different metabolic pathways of the cell. The amount and nature of these molecules being the result of gene expression, the metabolite content of a cell is thus closely related to its phenotype. Metabolomics, which aims at studying the whole set of metabolites present in a biological sample, is an invaluable source of information for the understanding of metabolism.This is why the work presented in this thesis reports the setup of a metabolomic approach at Genoscope for the study of metabolism of the model bacterium Acinetobacter baylyi ADP1 (ADP1) through high-resolution mass spectrometry (LTQ-Orbitrap) coupled to liquid chromatography (LC/MS).We investigated the metabolism of ADP1 after an environmental perturbation induced by a change of carbon source. We compared the metabolomes of ADP1 grown on succinate (reference carbon source) and on quinate (alternative source). About 450 potential metabolites could be detected and more than a hundred were identified. Many observations were made. First, quinate as a carbon source triggered an expected specific metabolic response in relation with its dissimilation (quinate catabolites were detected and the corresponding genes were up-regulated). However, while central metabolism is not impacted by this biotic change, about half of the detected metabolites had their concentration changed. This unexpected result is consistent with transcriptomic experiments that revealed that with the change of carbon source, 12% of the total number of genes are differentially expressed. Our results show that in these conditions, metabolic alteration goes far beyond the quinate degradation pathway and causes a global overhaul of the metabolism of ADP1 (Stuani, Lechaplais et al. 2014).This study also led to the detection of novel metabolites that were exclusively produced in quinate-grown cells. The structural elucidation of one of them is reported in this manuscript. It is a tyrosine substituted in the benzylic position by an aminomethyl group (BMAT). Its identification required sequential CID-MSn experiments with high-resolution detection and isotopic H/D exchange experiments. Structural studies conducted on two other molecules suggest structural similarities with BMAT. We thus could be in the presence of a family of novel secondary metabolites, involved in the same metabolic pathway.In conclusion, a metabolomic approach allowed a mere change of carbon source to guide us toward the discovery of novel metabolic pathways and gain insight into the hidden part of ADP1 metabolism
Tan, Mengmeng. "Structural optimization of polypod-like structured DNA based on structural analysis and interaction with cells". Kyoto University, 2020. http://hdl.handle.net/2433/253233.
Pełny tekst źródłaSibai, Munira. "Optimization of an Unfurlable Space Structure". Thesis, Virginia Tech, 2020. http://hdl.handle.net/10919/99908.
Pełny tekst źródłaMaster of Science
Spacecraft, or artificial satellites, do not fly from earth to space on their own. They are launched into their orbits by placing them inside launch vehicles, also known as carrier rockets. Some parts or components of spacecraft are large and cannot fit in their designated space inside launch vehicles without being stowed into smaller volumes first. Examples of large components on spacecraft include solar arrays, which provide power to the spacecraft, and antennas, which are used on satellite for communication purposes. Many methods have been developed to stow such large components. Many of these methods involve folding about joints or hinges, whether it is done in a simple manner or by more complex designs. Moreover, components that are flexible enough could be rolled or wrapped before they are placed in launch vehicles. This method reduces the mass which the launch vehicle needs to carry, since added mass of joints is eliminated. Low mass is always desirable in space applications. Furthermore, wrapping is very effective at minimizing the volume of a component. These structures store energy inside them as they are wrapped due to the stiffness of their materials. This behavior is identical to that observed in a deformed spring. When the structures are released in space, that energy is released, and thus, they deploy and try to return to their original form. This is due to inertia, where the stored strain energy turns into kinetic energy as the structure deploys. The physical analysis of these structures, which enables their design, is complex and requires computational solutions and numerical modeling. The best design for a given problem can be found through numerical optimization. Numerical optimization uses mathematical approximations and computer programming to give the values of design parameters that would result in the best design based on specified criterion and goals. In this thesis, numerical optimization was conducted for a simple unfurlable structure. The structure consists of a thin rectangular panel that wraps tightly around a central cylinder. The cylinder and panel are connected with a hinge that is a rotational spring with some stiffness. The optimization was solved to obtain the best values for the stiffness of the hinge, the thickness of the panel, which is allowed to vary along its length, and the stiffness or elasticity of the panel's material. The goals or objective of the optimization was to ensure that the deployed panel meets stiffness requirement specified for similar space components. Those requirements are set to make certain that the spacecraft can be controlled from earth even with its large component deployed. Additionally, the second goal of the optimization was to guarantee that the unfurling panel does not have very high energy stored while it's wrapped, so that it would not cause large motion the connected spacecraft in the zero gravity environments of space. A computer simulation was run with the resulting hinge stiffness and panel elasticity and thickness values with the cylinder and four panels connected to a structure representing a spacecraft. The simulation results and deployment animation were assessed to confirm that desired results were achieved.
Swanson, Gary D. "Structural efficiency study of composite wing rib structures". Thesis, This resource online, 1987. http://scholar.lib.vt.edu/theses/available/etd-04292010-020010/.
Pełny tekst źródłaLiu, Wenjie. "Structural dynamic analysis and testing of coupled structures". Thesis, Imperial College London, 2001. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.246801.
Pełny tekst źródłaYang, Nana. "Structural strength and reliability analysis of composite structures". Thesis, University of Strathclyde, 2010. http://oleg.lib.strath.ac.uk:80/R/?func=dbin-jump-full&object_id=13242.
Pełny tekst źródłaBennett, J. E. "Structural and compositional studies of novel ribbon structures". Thesis, University of Cambridge, 2002. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.596574.
Pełny tekst źródłaDawood, Tariq Ali. "Structural health monitoring of GFRP sandwich beam structures". Thesis, University of Southampton, 2006. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.438529.
Pełny tekst źródłaSamad, Abdus. "Structural and magnetic properties of spin valve structures". Thesis, University of Cambridge, 1999. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.624580.
Pełny tekst źródłaEdrees, Tarek. "Structural Control and Identification of Civil Engineering Structures". Doctoral thesis, Luleå tekniska universitet, Byggkonstruktion och -produktion, 2015. http://urn.kb.se/resolve?urn=urn:nbn:se:ltu:diva-18700.
Pełny tekst źródłaGodkänd; 2015; 20150303 (taredr); Nedanstående person kommer att disputera för avläggande av teknologie doktorsexamen. Namn: Tarek Edreees Saaed Alqado Ämne: Konstruktionsteknik/Structural Engineering Avhandling: Structural Control and Identification of Civil Engineering Structures Opponent: Professor Francesc Pozo, Department of Applied Mathematics III, Escola Universitària d’Enginyeria Tècnica Industrial de Barcelona (EUETIB), Universitat Politècnica de Catalunya Comte d’Urgell, Barcelona, Spanien Ordförande: Professor Jan-Erik Jonasson vid Avd för byggkonstruktion och produktion, Institutionen för samhällsbyggnad och naturresurser, Luleå tekniska universitet Tid: Torsdag den 26 mars 2015, kl 10.00 Plats: C305, Luleå tekniska universitet
Ullah, Israr. "Vibration-based structural health monitoring of composite structures". Thesis, University of Manchester, 2011. https://www.research.manchester.ac.uk/portal/en/theses/vibrationbased-structural-health-monitoring-of-composite-structures(f21abb03-5b46-4640-9447-0552d5e0c7d6).html.
Pełny tekst źródłaShafieezadeh, Abdollah. "Application Of Structural Control For Civil Engineering Structures". DigitalCommons@USU, 2008. https://digitalcommons.usu.edu/etd/142.
Pełny tekst źródłaNunes, Eliana Ferreira. "Qualitative investigation of the performance of a structural membrane roof project". reponame:Repositório Institucional da UFOP, 2012. http://www.repositorio.ufop.br/handle/123456789/6036.
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This paper provides a qualitative investigation about the structural performance of the membranes, surface structures (with double curvature in opposite directions) with minimum thickness and weight, which absorb forces in form of tensile stresses in its own plane, considering two aspects: structural and design procedure. Initially, it involved the analyses of lightweight structure buildings and the observation of constructive work process in membrane roofs. These investigations allowed identifying strategies that contribute to achieve optimum system performance and the challenges encountered along the stages of designing and building. They also guided the qualitative analysis of the performance of a structural membrane roofing project, i.e., a particular situation, as example. This qualitative analysis was developed in two stages, guided by experimental and numerical data. The first stage involved the optimization procedure of the structural system under load action. This analysis showed that the flexible system performance is a result of the three-dimensional stability of the structural system (arrangement and geometry of all components), membrane surface stiffness (membrane geometry), as well as the cooperation of all components in pre-tension state. The second stage comprised the experimental investigation of the membrane material behaviour within the structure context in order to analyze the flattened membrane geometry. Such evaluation enabled to verify the difference between the theoretical model (shape of equilibrium) and the actual shape (consisting of flat panels), enabling the proper adjustment of the surface geometry so that the final shape can reveal not only the path of the forces, but also the best use of the material. The investigations, analyses and working procedure here adopted broadened the understanding of this system pointing possibilities to increase its performance and to minimize failures during the preliminary stage of design.
Gabriel, Christopher. "Effect of localized structural perturbations on dendrimer structure". Columbus, Ohio : Ohio State University, 2006. http://rave.ohiolink.edu/etdc/view?acc%5Fnum=osu1155662110.
Pełny tekst źródłaLea, Patrick D. "Fluid Structure Interaction with Applications in Structural Failure". Thesis, Northwestern University, 2014. http://pqdtopen.proquest.com/#viewpdf?dispub=3605735.
Pełny tekst źródłaMethods for modeling structural failure with applications for fluid structure interaction (FSI) are developed in this work. Fracture as structural failure is modeled in this work by both the extended finite element method (XFEM) and element deletion. Both of these methods are used in simulations coupled with fluids modeled by computational fluid dynamics (CFD). The methods presented here allow the fluid to pass through the fractured areas of the structure without any prior knowledge of where fracture will occur. Fracture modeled by XFEM is compared to an experimental result as well as a test problem for two phase coupling. The element deletion results are compared with an XFEM test problem, showing the differences and similarities between the two methods.
A new method for modeling fracture is also proposed in this work. The new method combines XFEM and element deletion to provide a robust implementation of fracture modeling. This method integrates well into legacy codes that currently have element deletion functionality. The implementation allows for application by a wide variety of users that are familiar with element deletion in current analysis tools. The combined method can also be used in conjunction with the work done on fracture coupled with fluids, discussed in this work.
Structural failure via buckling is also examined in an FSI framework. A new algorithm is produced to allow for structural subcycling during the collapse of a pipe subjected to a hydrostatic load. The responses of both the structure and the fluid are compared to a non-subcycling case to determine the accuracy of the new algorithm.
Overall this work looks at multiple forms of structural failure induced by fluids modeled by CFD. The work extends what is currently possible in FSI simulations.
Barzegari, Mohamad Reza. "Structure-flexural modulus relationships in polymeric structural foams". Thesis, Université Laval, 2009. http://www.theses.ulaval.ca/2009/26347/26347.pdf.
Pełny tekst źródłaGabriel, Christopher J. "Effect of localized structural perturbations on dendrimer structure". The Ohio State University, 2006. http://rave.ohiolink.edu/etdc/view?acc_num=osu1155662110.
Pełny tekst źródłaGlick, David A. "Structure and Objects: A Defense of Structural Realism". Diss., The University of Arizona, 2014. http://hdl.handle.net/10150/322984.
Pełny tekst źródłaLee, Seung-Yoon. "Geometrically exact modeling and nonlinear mechanics of highly flexible structures /". free to MU campus, to others for purchase, 2002. http://wwwlib.umi.com/cr/mo/fullcit?p3074421.
Pełny tekst źródłaThakur, Sudhir K. "Structure and structural changes in India: A fundamental economic structure approach". The Ohio State University, 2004. http://rave.ohiolink.edu/etdc/view?acc_num=osu1092857658.
Pełny tekst źródłaRungthonkit, Prathan. "Structural behaviour of structural insulated panels (SIPS)". Thesis, University of Birmingham, 2012. http://etheses.bham.ac.uk//id/eprint/3561/.
Pełny tekst źródłaLannamann, Daniel L. "Structural health monitoring : numerical damage predictor for composite structures". Thesis, Monterey, Calif. : Springfield, Va. : Naval Postgraduate School ; Available from National Technical Information Service, 2001. http://handle.dtic.mil/100.2/ADA390997.
Pełny tekst źródłaNayyerloo, Mostafa. "Real-time Structural Health Monitoring of Nonlinear Hysteretic Structures". Thesis, University of Canterbury. Department of Mechanical Engineering, 2011. http://hdl.handle.net/10092/6581.
Pełny tekst źródłaBruun, Karianne. "Structural Dynamics of Subsea Structures in Earthquake Prone Regions". Thesis, Norges teknisk-naturvitenskapelige universitet, Institutt for konstruksjonsteknikk, 2013. http://urn.kb.se/resolve?urn=urn:nbn:no:ntnu:diva-24328.
Pełny tekst źródłaEksik, Ömer. "Structural performance of GRP top hat stiffened marine structures". Thesis, University of Southampton, 2005. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.431952.
Pełny tekst źródłaMa, Yanhang. "Structural study of nano-structured materials: electron crystallography approaches". Doctoral thesis, Stockholms universitet, Institutionen för material- och miljökemi (MMK), 2016. http://urn.kb.se/resolve?urn=urn:nbn:se:su:diva-129233.
Pełny tekst źródłaKirikera, Goutham Raghavendra. "A Structural Neural System for Health Monitoring of Structures". University of Cincinnati / OhioLINK, 2006. http://rave.ohiolink.edu/etdc/view?acc_num=ucin1155149869.
Pełny tekst źródłaIslami, Kleidi. "System identification and structural health monitoring of bridge structures". Doctoral thesis, Università degli studi di Padova, 2013. http://hdl.handle.net/11577/3423079.
Pełny tekst źródłaQuesto lavoro di ricerca mira a due obiettivi per l'identificazione delle caratteristiche strutturali dei sistemi infrastrutturali civili. Il primo è legato al problema della identificazione del sistema dinamico, mediante analisi modale sperimentale e operativa, applicata ad una grande varietà di strutture da ponte. Basandosi su tecniche nel dominio del tempo e delle frequenze e, soprattutto, su dati di output di accelerazione, velocità o strain, i parametri modali sono stati stimati per ponti sospesi, ponti ad arco in muratura, ponti a travi in calcestruzzo e ad arco, ponti reticolari e ponti in acciaio a cassone. Dopo aver dato una panoramica approfondita dei metodi stocastici standard ed avanzati, sono state evidenziate le differenze degli approcci esistenti nelle loro performance per l'identificazione del sistema sui diversi tipi di infrastrutture civili. La valutazione della loro performance viene accompagnata da casi facilmente e difficilmente determinabili, che hanno dato buoni risultati solo dopo l'esecuzione di analisi avanzate di Clustering. Inoltre, sono stati sviluppati algoritmi di identificazione dinamica automatica in tempo reale basandosi sulle vibrazioni strutturali dei ponti monitorati, a sua volta utilizzati nel rilevamento dei danni strutturali tramite modelli statistici. Il secondo problema studiato riguarda la stima di spostamenti di ordine superiore che si svolgono sui ponti sospesi, eliminando il rumore di misura e di processo. Una volta fornito un trattamento completo della fusione dei dati di spostamento e accelerazione per i sistemi dinamici tramite il filtro di Kalman, la combinazione di questi due tipi di misurazioni ha mostrato un miglioramento nelle deformazioni osservate. Pertanto, è stata presentata un'analisi esauriente di un ponte sospeso e dei sui dati dinamici e di spostamento filtrati. I test positivi sono stati successivamente utilizzati per definire il problema dei sensori non collocati alla stessa locazione ed applicazione su modelli semplificati
Woestenenk, Esmeralda A. "Protein production, characterization and structure determination in structural genomics". Doctoral thesis, KTH, Biotechnology, 2004. http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-29.
Pełny tekst źródłaThis thesis covers the process from expression of a heterologous gene in Escherichia coli to structure determination of a protein by nuclear magnetic resonance (NMR) spectroscopy.
The first part concerns structural genomics-related parallel screening studies on the effect of fusion tags (in particular the His tag) on protein solubility and the use of fusion tags in fast, parallel purification protocols intended for initial biophysical characterization of human proteins produced in E. coli. It was found that for most proteins the His tag has a negative influence on protein solubility. This influence appears to be more pronounced for our C-terminal His tag than for the N-terminal His tags used in this study. Moreover, high ratios of soluble per total protein do not always guarantee a high yield of soluble protein after purification, as different vector - target protein combinations result in large differences in host cell growth rates. Protein purification protocols for different fusion tags were developed that make it possible to express, purify and study structural properties of low concentration samples of 15N-labeled proteins in one or two days.
The second part of this thesis describes the assignment and solution structure determination of ribosomal protein L18 of Thermus thermophilus. The protein is a mixed α/β structure with two α-helices on one side of a four-stranded β-sheet. Comparison to RNA-bound L18 showed that the protein to a large extent adopts identical structures in free and bound states, with exception of the loop regions and the flexible N-terminus.
Keywords: protein production, protein solubility, fusion tags, nuclear magnetic resonance, structure determination, ribosomal protein
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Pełny tekst źródłaCommittee Chair: Yannis Smaragdakis; Committee Member: Oege de Moor; Committee Member: Richard LeBlanc; Committee Member: Santosh Pande; Committee Member: Spencer Rugaber. Part of the SMARTech Electronic Thesis and Dissertation Collection.