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Merino, Soto Cesar, i Pradhan Rabindra Kumar. "Validación estructural del R-SPQ-2F: Un análisis factorial confirmado". Universidad Peruana de Ciencias Aplicadas (UPC), 2014. http://hdl.handle.net/10757/325056.
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El motivo del presente estudio es validar la estructura interna del Cuestionario Revisado de Procesos de Estudio, Dos factores (Revised-Study Process Questionnaire-2 Factors; Biggs, Kember & Leung, 2001), en una muestra heterogénea de 269 participantes universitarios y no universitarios. Se aplicó la metodología de ecuaciones estructurales para probar varios modelos de medición: unidimensional, cuatro factores relacionados y con estructura jerárquica, y dos factores (ortogonales y oblicuos). Se halló que los modelos basados en cuatro factores no mostraron un ajuste satisfactorio, y uno tuvo problemas de convergencia ocasionados por la muy alta correlación interfactorial. El modelo de dos factores (profundo y superficial), con errores correlacionados, tuvo satisfactorio ajuste. La consistencia interna también fue satisfactoria. Se discute la utilidad del instrumento y su reformulación teórica.Revisión por pares
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Costa, Simone de Godoy. "Validação para o Brasil da escala Revised two-factor Study Process Questionnaire (R-SPQ-2F)". Universidade de São Paulo, 2010. http://www.teses.usp.br/teses/disponiveis/83/83131/tde-26042010-143400/.
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Frente à escassez de investigações experimentais que enfoquem o processo de estudo entre universitários de enfermagem, na literatura nacional e, dada a indicação da necessidade de que tal temática seja constantemente considerada para que as instituições educacionais consigam preparar recursos humanos de enfermagem adequados à realidade brasileira, apresentou-se esta proposta de investigação cujo objetivo foi o de validar para o Brasil a escala Revised two-factor Study Process Questionnaire (R-SPQ-2F). O questionário possui 20 itens distribuídos em duas escalas com 10 itens cada, sendo uma de Abordagem Profunda (DA) e outra de Abordagem Superficial (SA). Cada escala possui duas subescalas compostas de cinco itens cada, sendo duas referentes à motivação e duas à estratégia: motivação profunda (dm), estratégia profunda (ds), motivação superfcicial (sm) e estratégia superficial (ss). Os dados foram coletados em uma escola de enfermagem de uma universidade pública estadual do interior paulista, no período de julho a dezembro de 2008, tendo participado do estudo 202 estudantes do curso de bacharelado em enfermagem. As propriedades psicométricas analisadas foram: validade de face e de conteúdo (comitê de juízes); confiabilidade (consistência interna alfa de Cronbach); validade de construto (análise fatorial confirmatória) e validade de critério (notas em disciplinas curriculares). A maioria dos estudantes era do sexo feminino 194 (96%) com idade média de 22,3 anos (DP=2,1). O processo de revisão pelo comitê de juízes e validação semântica ocorreu sem intercorrências significativas. A confiabilidade da versão adaptada foi considerada muito boa devido a alta consistência interna de seus itens, segundo os valores de alfa de Cronbach de 0,76 para DA e 0,74 para SA. Em relação à validade de construto, a análise fatorial confirmatória indicou um ajuste muito satisfatório em relação ao modelo que testa a dimensionalidade de todo o instrumento e trata as subescalas como indicadores dos fatores latentes DA e SA. Assim, a versão adaptada do R-SPQ-2F apresentou medidas psicométricas consideradas satisfatórias, estando adequado para a utilização como uma ferramenta de avaliação da abordagem ao estudo de estudantes de enfermagem brasileiros.Due to the lack of experimental research focusing the study process among nursing university students, in Brazilian literature and, considering the need of this theme being constantly thought of in order that the educational institutions can prepare human resources in nursing appropriate to the Brazilian reality, this researched aimed to validate the scale Revised two-factor Study Process Questionnaire (R-SPQ- 2F) for Brazil. The questionnaire has 20 items distributed in two scales with 10 items each, one with Deep Approach (DA) and the other with Surface Approach (SA). Each scale has two subscales consisting of five items each, two regarding the motivation and two the strategy: deep motive (dm), deep strategy (ds), surface motive (sm) and surface strategy (ss). Data were collected in a nursing college from a public state university in the interior of the state of São Paulo, between July and December 2008. In total, 202 students from the Nursing Bachelor\'s Program participated in study. The following psychometric properties were analyzed: face and content validity (committee of experts); reliability (Cronbach\'s alpha internal consistency); validity of construct (confirming factorial analysis) and validity of criteria (grades in syllabus courses). Most students were female 194 (96%) aged on average 22.3 years (SD=2.1). The review process by the committee of experts and semantic validation did not have significant incidents. The reliability of the adapted version was considered very good due to the high internal consistency of its items, according to the values of Cronbach\'s alpha of 0.76 for DA and 0.74 for SA. Regarding the validity of construct, the confirming factorial analysis showed a very satisfactory adjustment regarding the model that tests the dimensionality of the whole instrument and treats the subscales as indicators of the latent factors DA and SA. Thus, the adapted version of the R-SPQ-2F presented psychometric measures considered satisfactory and is appropriate to be used as a tool to assess the approach of the study of Brazilian Nursing students.
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Defelippe, Raisa Coppola. "Tradução e adaptação cultural para a língua portuguesa do Brasil do Sensory Perception Quotient (SPQ)". Universidade Presbiteriana Mackenzie, 2016. http://tede.mackenzie.br/jspui/handle/tede/2961.
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Considering that approximatelly 90% of individuals with ASD show some deficit in sensory perception and the adition of atypical sensory criteria in Diagnostic and Statistical Manual of Mental Disorder, the fifith edition (DSM-5) for diagnosis of ASD, there is a need to have instruments that can access this constructs enabiling more research. Many exiting instruments are questionnaires for parents, and most of them focus on children, therefore little is known about sensory perception in adults. Given the lack of brazilian intruments to evaluate the hability of sensory processing and questionnaires that can access the real experience of who lives this reality, this study aims to translate to brazilian portuguese and culturally adapt the Sensory Perception Quotient, an instrument of self report that can access sensory reactivity in adults, evaluating five basic modalities, that can be hypo or hyper –reactive, avoiding value judgment. The purpose of this study, therefore, is to translate to brazilian portuguese and adapt culturally to the brazilian culture of the Sensory Perception Quotient, conducting a study within a scope of a non-experimental non-longitudinal descriptive research, and the following steps: translation, back-translation, revision by a specialist comittee on analysis, evaluation of he scoring and the verification of the instrument’s internal consistency. Sample was constituied of 87 participants, ages 18 to 52 years. The results obtained by the application of total questionnaire translated and adapted, already modified in 53 questions in relation to it’s original version, aloud to notice that it is understandable and shows intern consistency little under of desirable, having only the short version with desirable intern consistency. These observations indicate that the search for evidence of validy of the short version of the instrument, realized through new researches, with other applications of the translated version and adapted in this study, will make it possible to applicate in Brazil. Is recommened caution with full questionnaire in the disscution part of this study.
Considerando-se o fato de que aproximadamente 90% dos indivíduos com TEA mostram algum déficit na percepção sensorial junto ao fato da inclusão do critério sensorial atípico no Manual de Diagnóstico e Estatística dos Transtornos Mentais 5.ª edição (DSM-5) para diagnóstico de TEA, há necessidade de se ter instrumentos que possam acessar esses constructos possibilitando mais pesquisas. Muitos instrumentos existentes são questionários para pais, e a maioria se volta para crianças, assim pouco se sabe sobre a percepção sensorial em adultos. Diante da falta de instrumentos brasileiros para avaliar a habilidade de processamento sensorial e questionários que possam acessar a experiência daquele que vive diretamente essa realidade, este estudo objetivou traduzir para o português do Brasil e adaptar culturalmente o Sensory Perception Quotient, um instrumento de auto relato que acessa a reatividade sensorial em adultos, avaliando as cinco modalidades básicas, podendo ser hipo ou hiper-reativo, evitando julgamento de valores. O presente estudo se encaixa no modelo de pesquisa não experimental, transversal, descritiva, contendo os seguintes passos: tradução, retrotradução, revisão por um comitê de especialistas, avaliação das pontuações e verificação da consistência interna do instrumento. A amostra foi constituída de 87 participantes, com idades entre 18 a 52 anos. Os resultados obtidos através da aplicação do questionário total traduzido e adaptado, já com mudanças em 53 perguntas em relação à versão original, permitiram observar que ele é compreensível e apresenta consistência interna pouco abaixo do desejável, apenas com a versão reduzida (short) possuindo consistência interna adequada. Essas observações indicam que a busca de evidências de validade do instrumento da versão reduzida, realizada através de novas pesquisas, com outras aplicações da versão traduzida e adaptada neste estudo poderá tornar possível sua utilização no Brasil. Recomenda-se cautela maior com o questionário total na discussão do trabalho.
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Stenegren, Erik. "Somatosensory attenuation : Differences in the attenuation of self-generated touch in terms of intensity, pleasantness and ticklishness". Thesis, Högskolan i Skövde, Institutionen för biovetenskap, 2021. http://urn.kb.se/resolve?urn=urn:nbn:se:his:diva-20186.
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The phenomenon of somatosensory attenuation describes the perception that self-generated touch feels weaker than externally generated touch of identical intensity. Previous studies have shown that besides intensity, self-generated touches feel less pleasant and less ticklish than identical externally generated touches. However, previous studies did not systematically assess attenuation across a range of stimuli that can elicit intensity, pleasantness, and ticklishness more efficiently. This thesis aims to replicate these previous observations across a range of tactile stimuli of different intensities and velocities and investigate whether people who attenuate their self-generated touches to a greater extent do so for all aforementioned qualities. Previous studies have shown that participants with lower levels of somatosensory attenuation have more schizotypal personality traits. Twelve volunteers participated in three perceptual tasks where they received touches on their sole generated either by a robot(External) or the participants(Self). Following the strokes, participants had to rate the sensation from 0(not at all) to 100(extremely). For the intensity task, we manipulated the intensity of the applied forces (1,2,3,4N). For the pleasantness and ticklishness tasks, we manipulated the velocity of the applied strokes (0.3,1,10,30cm/s). After the tasks, participants completed a Schizotypal Personality Questionnaire (SPQ). Significant somatosensory attenuation was observed in all tasks, but for specific, not all, stimuli: forces of 4N in terms of intensity, strokes of 1cm/s in terms of pleasantness, and strokes of 10cm/s and 30cm/s in terms of ticklishness. These results suggest that the ability to suppress the tactile consequences of self-generated touch occurs in all three tactile qualities.
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Al, Nzawi Fatma Mohammed. "Cultural factors influencing content of delusions among schizophrenic patients in Saudi Arabia". Thesis, Brunel University, 2012. http://bura.brunel.ac.uk/handle/2438/7630.
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The present study aimed to investigate the influences of socio-cultural context on content of delusions within schizophrenic patients among three different geographical areas in Saudi Arabia and to estimate the prevalence of schizotypal personality within the non-clinical Saudi population. Methods: 148 patients meeting DSM-IV criteria of schizophrenia were drawn from in-patient sections of mental health hospitals in three areas of study (Riyadh, Jeddah, and the Eastern Province), and 364 participants were recruited for comparison groups from Saudi universities and general medical practices in the same areas. The study utilized the following instruments: Scale for the Assessment of Positive Symptoms (SAPS), Scale for the Assessment of Negative Symptoms (SANS), the MacArthur-Maudsley Delusions Assessment Schedule (MMDAS), the World Health Organization (WHO) Life Events Schedule, the Aetiological Beliefs Questionnaire (ABQ), and the Schizotypal-Personality Questionnaire (SPQ). Results: Firstly: there were significant main effects of residence and sex on the three dependent SPQ scales, and females and Riyadh area residents scored higher on all dependent SPQ scales especially on the cognitive scale. Secondly: persecutory delusions were the most common delusions among schizophrenic patients followed by delusions of being controlled, and the frequency of persecutory delusions was significantly higher in females and in the Riyadh area. Thirdly: only delusions of jealousy were specifically associated with violent behaviours among schizophrenic patients. Finally: ABQ showed that both supernatural and psychological explanations of illness were common among both control and clinical groups. Qualitative analysis of the content of delusions revealed socio-cultural and gender-related issues that were strongly reflected in the themes and content of delusions. Conclusion: Content analysis of delusions in a Saudi schizophrenic sample showed strong effects of the socio-cultural context and stress points such as racism and gender inequality, and gender and regional culture affected both the frequency with which persecutory delusions were observed, and the mean scores of schizotypal personality in a non-clinical sample.
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Jones, Catherine Toni, i n/a. "Biggs's 3P Model of Learning: The Role of Personal Characteristics and Environmental Influences on Approaches to Learning". Griffith University. School of Applied Psychology, 2003. http://www4.gu.edu.au:8080/adt-root/public/adt-QGU20030304.092316.
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The aim of this research programme was to examine the 3P model of learning (Biggs, 1987a, 1999). The first stage necessarily involved an examination of the Study Process Questionnaire (SPQ) (Biggs, 1987a), an instrument developed to measure the process component of the model. The structure of the SPQ was examined utilising exploratory and confirmatory factor analysis of undergraduate responses (n= 260). The results indicated the higher-order factor structure of deep-achieving and surface-achieving-motive provided the most reliability and a better model fit than either the subscales or scales of the SPQ. The construct validity of the two constructs deep and surface was assessed next using a multitrait-multimethod matrix (MTMM) constructed from the three measures of the self-report questionnaire, interview ratings and written assessments from first-year students (n = 50). The results indicated good convergent validity between the deep scale of the SPQ and the interview ratings on the deep scale, between the deep scale on the SPQ and the written assessment ratings, and between the interview ratings and written assessment ratings. The results indicated good convergent validity between the surface scale on the SPQ and the interview ratings on the surface scale, but not between the surface scale on the SPQ and the written assessment ratings, and between the interview ratings and written assessment ratings. The discriminant validity between deep and surface was good for the SPQ, but not for either the interview or the written assessment. The findings indicate the deep and surface scales of the SPQ adequately measure the underlying deep and surface constructs. The retest reliability of the SPQ was then examined utilising Spearmans Rho to assess the rank-order correlations with a sample of third-year students (n=87). Over a period of three months there were significant correlations for the surface motive, surface strategy, deep strategy, achieving motive and achieving strategy subscales of the SPQ, suggesting good reliability for these subscales. The results at the scale level of the SPQ result in similar conclusions. There was a moderate significant correlation for the surface, deep and achieving scales of the SPQ, suggesting the scales have good reliability over a period of three months. There was also a moderate significant correlation for the surface-achieving-motive and deep-achieving scales over a period of three months. The stability of SPQ scores was also assessed utilising a series of one-way repeated measures MANOVAs with a sample of third-year undergraduates (n = 64). The results suggest some change occurs in self-reported use of approaches to learning between the first and third-years of an undergraduate degree programme. The role of the teaching-learning environment was next examined. Utilising a within-subjects design, undergraduate students (n=48) concurrently enrolled in traditional (viz. lecture and tutorial) and non-traditional (viz. workshops and group projects) subjects completed the SPQ to describe their approaches to learning in each subject. A series of 2x2 repeated measures MANOVAs were undertaken. The results indicated students were likely to change their approach to learning based on their perceptions of the learning environment (traditional or non-traditional subject). However, those students identified as predominantly surface learners significantly increased their deep scale scores in the non-traditional subject when compared to deep learners. The next study examined a range of personality (locus of control, sensing function, thinking function, intelligence) and demographic variables (age, gender, year of study) to assess which were good predictors of deep and surface approaches to learning. A series of regression analyses identified age, sensing function and locus of control as significant predictors of the surface, surface-achieving-motive, and deep approaches to learning. Locus of control was found to be a significant predictor of the deep-achieving approach to learning. The final study examined the 3P model of learning. Based on the results of earlier studies in the research programme the situational component of the presage factors was not included. The model was examined using structural equation modelling (n= 394). Two initial models were tested using both the three (deep, surface, achieving) and two (surface-achieving-motive and deep-achieving) process factor models. The three process factor model provided the better model fit. The results suggest deep and surface approaches to learning do not mediate between personal characteristics and learning outcomes (i.e. GPA). The results of this series of studies suggest the need for further research into the SPQ and the 3P model of learning. The implications of the research programme are also discussed.
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Jones, Catherine Toni. "Biggs's 3P Model of Learning: The Role of Personal Characteristics and Environmental Influences on Approaches to Learning". Thesis, Griffith University, 2003. http://hdl.handle.net/10072/366357.
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The aim of this research programme was to examine the 3P model of learning (Biggs, 1987a, 1999). The first stage necessarily involved an examination of the Study Process Questionnaire (SPQ) (Biggs, 1987a), an instrument developed to measure the process component of the model. The structure of the SPQ was examined utilising exploratory and confirmatory factor analysis of undergraduate responses (n= 260). The results indicated the higher-order factor structure of deep-achieving and surface-achieving-motive provided the most reliability and a better model fit than either the subscales or scales of the SPQ. The construct validity of the two constructs deep and surface was assessed next using a multitrait-multimethod matrix (MTMM) constructed from the three measures of the self-report questionnaire, interview ratings and written assessments from first-year students (n = 50). The results indicated good convergent validity between the deep scale of the SPQ and the interview ratings on the deep scale, between the deep scale on the SPQ and the written assessment ratings, and between the interview ratings and written assessment ratings. The results indicated good convergent validity between the surface scale on the SPQ and the interview ratings on the surface scale, but not between the surface scale on the SPQ and the written assessment ratings, and between the interview ratings and written assessment ratings. The discriminant validity between deep and surface was good for the SPQ, but not for either the interview or the written assessment. The findings indicate the deep and surface scales of the SPQ adequately measure the underlying deep and surface constructs. The retest reliability of the SPQ was then examined utilising Spearman’s Rho to assess the rank-order correlations with a sample of third-year students (n=87). Over a period of three months there were significant correlations for the surface motive, surface strategy, deep strategy, achieving motive and achieving strategy subscales of the SPQ, suggesting good reliability for these subscales. The results at the scale level of the SPQ result in similar conclusions. There was a moderate significant correlation for the surface, deep and achieving scales of the SPQ, suggesting the scales have good reliability over a period of three months. There was also a moderate significant correlation for the surface-achieving-motive and deep-achieving scales over a period of three months. The stability of SPQ scores was also assessed utilising a series of one-way repeated measures MANOVA’s with a sample of third-year undergraduates (n = 64). The results suggest some change occurs in self-reported use of approaches to learning between the first and third-years of an undergraduate degree programme. The role of the teaching-learning environment was next examined. Utilising a within-subjects design, undergraduate students (n=48) concurrently enrolled in traditional (viz. lecture and tutorial) and non-traditional (viz. workshops and group projects) subjects completed the SPQ to describe their approaches to learning in each subject. A series of 2x2 repeated measures MANOVA’s were undertaken. The results indicated students were likely to change their approach to learning based on their perceptions of the learning environment (traditional or non-traditional subject). However, those students identified as predominantly surface learners significantly increased their deep scale scores in the non-traditional subject when compared to deep learners. The next study examined a range of personality (locus of control, sensing function, thinking function, intelligence) and demographic variables (age, gender, year of study) to assess which were good predictors of deep and surface approaches to learning. A series of regression analyses identified age, sensing function and locus of control as significant predictors of the surface, surface-achieving-motive, and deep approaches to learning. Locus of control was found to be a significant predictor of the deep-achieving approach to learning. The final study examined the 3P model of learning. Based on the results of earlier studies in the research programme the situational component of the presage factors was not included. The model was examined using structural equation modelling (n= 394). Two initial models were tested using both the three (deep, surface, achieving) and two (surface-achieving-motive and deep-achieving) process factor models. The three process factor model provided the better model fit. The results suggest deep and surface approaches to learning do not mediate between personal characteristics and learning outcomes (i.e. GPA). The results of this series of studies suggest the need for further research into the SPQ and the 3P model of learning. The implications of the research programme are also discussed.Thesis (PhD Doctorate)
Doctor of Philosophy (PhD)
School of Applied Psychology
Full Text
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Pimentel, Telmo David Marcos. "O contributo dos sistemas integrados de gestão para o reconhecimento da excelência: um estudo das organizações premiadas pelo PEX-SPQ entre os anos 1994 e 2013". Master's thesis, [s.n.], 2015. http://hdl.handle.net/10284/4674.
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Dissertação apresentada à Universidade Fernando Pessoa como parte dos requisitos para a obtenção do grau de Mestre em Gestão da QualidadePela crecente importância dada aos sistemas integrados de gestão na atual literatura e pela evolução que os referenciais certificáveis têm registado no sentido da integração, o presente estudo pretende aferir a contribuição dos sistemas integrados de gestão na excelência. Para tal é verificada a presença, a contribuição e o efeito de melhoria patrocinada pelos sistemas de gestão nas organizações distinguidas pelo Prémio de Excelência do Sistema Português da Qualidade. Embora exista um grande número de estudos nas áreas dos diferentes referenciais e sistemas integrados de gestão, mediante a revisão bibliográfica efetuada foi possível constatar, em Portugal, a inexistência de estudos equivalentes àquele que esta dissertação se ocupa. A adoção do inquérito por questionário possibilitou a inquirição dos distinguídos no âmbito da excelência em Portugal e ultrapassar o problema da disperssão geográfica destas organizações. O tratamento estatístico aplicado aos dados recolhidos contribuiu para desmistificar a panaceia que ainda paira acerca das vantagens das diferentes certificações nas organizações, bem como atestar a sua eficácia enquanto agentes de melhoria.
By the increasing importance given to integrated management systems in the current literature and the developments that have been registered in certifiable standards towards integration, this study intends to evaluate the contribution of integrated management systems excellence. For this, the presence, contribution and the improving effect sponsored by the management systems in organizations distinguished by the Award of Excellence from the Portuguese Quality System is verified. Even though there are a large number of studies in the areas of the different standards and integrated management systems, based on the literature review which was carried out, it was established that in Portugal there is an absence of equivalent studies to the subject of this dissertation. The use of the questionnaire survey made it possible to inquire those distinguished within the excellence in Portugal and overcoming the geographical dispersion of these organizations. The statistical analysis applied to the data contributed to demystify the panacea that still hangs about the benefits of different certifications in organizations and attest to their effectiveness as improving agents.
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Bartninkienė, Danutė. "Specialiųjų poreikių mokinių integracija bendrojo lavinimo mokyklose: strateginis aspektas". Master's thesis, Lithuanian Academic Libraries Network (LABT), 2008. http://vddb.library.lt/obj/LT-eLABa-0001:E.02~2008~D_20080924_181957-45958.
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Valstybinės švietimo strategijos 2003–2012 metų nuostatose, patvirtintose Lietuvos Respublikos Seimo 2003 m. liepos 4 d. nutarimu Nr. IX-1700 (Žin., 2003, Nr.71-3216), nurodoma, kad siekiant užtikrinti švietimo plėtotės prieinamumą, tęstinumą ir socialinį teisingumą, turi būti užtikrinamos socialiai teisingos mokymosi ir studijų sąlygos, sukuriamos galimybės veiksmingai ugdyti įvairių gebėjimų ir poreikių vaikus.
Atlikus mokslinės literatūros analizę, galima pastebėti, kad apie specialiųjų poreikių asmenų integraciją visuomenėje yra rašę nemažai autorių: A. Galkienė, V. Rajeckas, G. Varoneckas, D. Žemaitytė, M. Barkauskaitė, J. Ambrukaitis, V. Gudonis ir kiti. Tačiau nepavyko aptikti duomenų apie integruoto ugdymo strateginį aspektą. Todėl moksliniame darbe norėta tai išsiaiškinti.
Šiame kontekste formuluojama magistrinio darbo tyrimo hipotezė: Jei galima identifikuoti specialiųjų poreikių vaikų integruoto ugdymo tendencijas ir problemas, tai galima parengti jų jų ugdymo bendrojo lavinimo mokyklose strategines gaires.
Tyrimo objektas: specialiųjų poreikių vaikų integruotas ugdymas bendrojo lavinimo mokyklose strateginiu aspektu. Tyrimo tikslas: sumodeliuoti specialiųjų poreikių vaikų ugdymo bendrojo lavinimo mokyklose strategines gaires.
Tyrimo uždaviniai: aptarti specialiųjų poreikių vaikų ugdymo Lietuvoje tendencijas; išryškinti specialiųjų poreikių vaikų integruoto ugdymo bendrojo lavinimo mokyklose problemas; empiriškai ištirti specialiųjų... [toliau žr. visą tekstą]In the 2003–2012 years regulations of national educational strategy which are confirmed by Seimas of the republic of Lithuania decision Number IX-1700 (Žin., 2003, Nr.71-3216) in 2003 July 4, it is stated that if you want to vouch accessibility, succession and social justice of educational development you must confirm the socially just learning conditions, create an effective opportunity to develop children who have different abilities and needs. According to analysis of nonfiction, it is possible to state that there are many authors who have written about integration of people who have special needs. They are A. Galkienė, V. Rajeckas, G. Varoneckas, D. Žemaitytė, M. Barkauskaitė, J. Ambrukaitis, V. Gudonis and the others. However, it was unsuccessful to find information about strategic aspect of integrated education. So it was tried to solve this in the work. The hypothesis of this postgraduate studies work is: if it is possible to identify integrated educational tendency and problems of children who have special needs, so it is possible to prepare strategic guideline of their development in comprehensive schools. An object of this research is integrated education of children who have special needs in comprehensive schools. The aim of research is to design the strategic guideline of children development that has special needs in comprehensive schools. The goals of this research are to discuss the tendencies of children, who have special needs, development in Lithuania; to... [to full text]
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Rousseaux, Sophie. "Palladium-Catalyzed C(sp2)-C(sp3) Bond Formation". Thèse, Université d'Ottawa / University of Ottawa, 2012. http://hdl.handle.net/10393/23058.
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Palladium-catalyzed reactions for carbon-carbon bond formation have had a significant impact on the field of organic chemistry in recent decades. Illustrative is the 2010 Nobel Prize, awarded for “palladium-catalyzed cross couplings in organic synthesis”, and the numerous applications of these transformations in industrial settings. This thesis describes recent developments in C(sp2)-C(sp3) bond formation, focusing on alkane arylation reactions and arylative dearomatization transformations. In the first part, our contributions to the development of intramolecular C(sp3)-H arylation reactions from aryl chlorides are described (Chapter 2). The use of catalytic quantities of pivalic acid was found to be crucial to observe the desired reactivity. The reactions are highly chemoselective for arylation at primary aliphatic C-H bonds. Theoretical calculations revealed that C-H bond cleavage is facilitated by the formation of an agostic interaction between the palladium centre and a geminal C-H bond. In the following section, the development of an alkane arylation reaction adjacent to amides and sulfonamides is presented (Chapter 3). The mechanism of C(sp3)-H bond cleavage in alkane arylation reactions is also addressed through an in-depth experimental and theoretical mechanistic study. The isolation and characterization of an intermediate in the catalytic cycle, the evaluation of the roles of both carbonate and pivalate bases in reaction mechanism as well as kinetic studies are reported. Our serendipitous discovery of an arylation reaction at cyclopropane methylene C-H bonds is discussed in Chapter 4. Reaction conditions for the conversion of cyclopropylanilines to quinolines/tetrahydroquinolines via one-pot palladium(0)-catalyzed C(sp3)-H arylation with subsequent oxidation/reduction are described. Initial studies are also presented, which suggest that this transformation is mechanistically unique from other Pd catalyzed cyclopropane ring-opening reactions. Preliminary investigations towards the development of an asymmetric alkane arylation reaction are highlighted in Chapter 5. Both chiral carboxylic acid additives and phosphine ligands have been examined in this context. While high yields and enantiomeric excesses were never observed, encouraging results have been obtained and are supported by recent reports from other research groups. Finally, in part two, the use of Pd(0)-catalysis for the intramolecular arylative dearomatization of phenols is presented (Chapter 7). These reactions generate spirocyclohexadienones bearing all-carbon quaternary centres in good to excellent yields. The nature of the base, although not well understood, appears to be crucial for this transformation. Preliminary results in the development of an enantioselective variant of this transformation demonstrate the influence of catalyst activation on levels of enantiomeric excess.
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Melo, Arthur Tavares de Oliveira. "Montagem e caracterização do transcritoma de cana-de-açúcar (saccharum spp.) utilizando dados de sequenciamento de nova geração". Universidade Federal de Goiás, 2015. http://repositorio.bc.ufg.br/tede/handle/tede/4783.
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Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPES
The sugarcane is one of the most important crop species to provide sugar and renewable energy in the world. Due to the high amount of repetitive elements and the various polyploidization events suffer during its evolution, the Saccharum spp. genome has not yet been assembled and annotated, unlike other agronomic important species. So, the knowledge about sugarcane transcriptome become even more useful for supporting genomic analyzes studies. A draft assembly of sugarcane transcriptome was obtained from Illumina sequencing paired-ends libraries of five different plant organs, sampled from thirty elite clones. Analyzes of quality control and normalization was done in the RNA-seq data. Trinity package was used for de novo assembly. The scaffolds obtained and identified as complete ORFs were annotated according to Gene Ontology terms. The draft assembly was characterized by the identification of microsatellites and SNPs molecular markers and for assessing the contribution of different plant organs for transcriptome final assembly. The draft sugarcane transcriptome comprised 178 Mb, over 131,831 scaffolds, representing 61,225 genes. The transcripts average size was 1,350 bp and N50 value was 1,667 bp. A total of 1,250 transcripts identified as complete ORFs showed no similarity to sequences of the nr NCBI database, are considered new Transcript Active Regions (nTARs). The annotation performed using the KEGG database identified 234 transcripts coding for enzymes members of sucrose and starch metabolism, an important metabolic pathway for understanding the relationship between photosynthetic rate and sucrose accumulation in the stalk. The five plant organs used contributed equally for the draft sugarcane transcriptome. A total of 12,931 genomic regions were identified containing perfect microsatellites, with a predominance of di and tri nucleotide. On average, one SNP every 18 bp was identified, with more than four million SNPs identified with satisfactory values of haplotype and quality scores. The nucleotide diversity of thirty elite clones used in this study was high. The identification of these molecular markers, particularly SNPs markers, provides the possibility of using these polymorphisms in genomic and genetic studies of sugarcane, including the possibility of application of genome wide selection like breeding strategy. The sugarcane transcriptome draft assembly proposed in this study has data and analysis quality sufficient to be used in attempt to encompass a reference transcriptome for the species of Saccharum spp.
A cana-de-açúcar é uma das principais espécies cultivadas para o fornecimento mundial de açúcar e energia renovável. Devido à elevada quantidade de elementos repetitivos e os vários eventos de poliploidização, o genoma da espécie ainda não foi montado e anotado, diferentemente de outras espécies de interesse agronômico. Assim, as informações do transcritoma da espécie se tornam ainda mais úteis por dar suporte ás iniciativas de análises genômicas. Um draft assembly do transcritoma de cana-de-açúcar foi montado a partir do sequenciamento Illumina de bibliotecas paired-ends de cinco órgãos distintos da planta, obtidos de uma amostra de trinta clones elite. Os dados de RNA-seq passaram por análises de controle de qualidade e normalização. O software Trinity foi utilizado para montagem de novo do transcritoma. Os scaffolds obtidos identificados como ORFs completas foram anotados conforme os termos do Gene Ontology. O draft assembly obtido para o transcritoma de cana-de-açúcar foi caracterizado pela identificação de marcadores moleculares do tipo microssatélites e SNPs e pela avaliação da contribuição dos diferentes órgãos vegetais para constituição final do transcritoma. O transcritoma obtido compreendeu 178 Mb, distribuídos em 131.831 scaffolds, representando 61.225 genes. O tamanho médio dos transcritos foi de 1.350 pb, com valor de N50 igual a 1.667 pb. Um total de 1.250 transcritos, identificados como ORFs completas, não apresentaram similaridade com sequências do banco de dados nr do NCBI, sendo considerados novas regiões transcricionalmente ativas (nTARs). A anotação realizada através do banco de dados do KEGG identificou 234 transcritos codificantes para enzimas integrantes do metabolismo de sacarose e amido, uma importante rota metabólica para compreensão da relação entre taxa fotossintética e o acúmulo de sacarose no colmo. Os cinco órgãos vegetais utilizados contribuíram igualmente para a constituição do draft do transcritoma de cana-de-açúcar. Foram identificadas 12.931 regiões genômicas contendo microssatélites perfeitos, com predomínio de di e tri nucleotídeos. Em média, identificou-se um SNP a cada 18 pares de bases, com mais de quatro milhões de SNPs identificados. A diversidade nucleotídica dos trinta clones elites utilizados é elevada. A identificação destes marcadores moleculares, principalmente os marcadores SNPs, fornece a possibilidade de utilização destes polimorfismos em estudos genéticos e genômicos de cana-de-açúcar, incluindo o emprego em abordagens como seleção genômica ampla no melhoramento da espécie. O draft assembly do transcritoma de cana-de-açúcar proposto neste estudo possui qualidade de dados e de análise suficiente para ser utilizado na tentativa de abranger um transcritoma de referência para as espécies de Saccharum spp.
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Шемедюк, Н. П. "Регуляція клітинного циклу GC-1 spg і GC-2 spd". Thesis, Київський національний університет технологій та дизайну, 2020. https://er.knutd.edu.ua/handle/123456789/15585.
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Broad, William. "Elucidating the function of the suppressor of ppi1 locus 2". Thesis, University of Oxford, 2017. https://ora.ox.ac.uk/objects/uuid:81cb5fb1-e735-453c-9c4b-332a5aa16b27.
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Tuthill, Matthew Charles. "N-myc oncogene expression in neuroblastoma is dependent on Sp1 and Sp3". Thesis, University of Hawaii at Manoa, 2003. http://hdl.handle.net/10125/987.
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Regulation of N-myc oncogene expression is an important determinant of the
biological behavior of neuroblastoma. The N-myc promoter contains several potential
binding sites for transcription factors of the Sp1 family. Mutation of a CT-box motif
contained within a 26 base pair region required for N-myc downregulation by retinoic
acid decreased basal transcriptional activity and altered DNA-protein interactions of the
promoter, while mutations flanking this motif did neither. On gel shift this region
generated 3 specific DNA-protein complexes that were reliant on wild type sequence of
the core CT element within it. Both Spl and Sp3 bound to the wild type probe as distinct
complexes in specifically retarded bands, while neither protein was present on mutated
sequences. Lysates from Drosophila S2 cells expressing exogenous Sp1 and Sp3
proteins were able to reproduce the gel shift complexes seen with neuroblastoma nuclear
extract. Transient transfections of S2 cells showed that individually or together, Sp1 and
Sp3 were able to trans-activate a N-myc CT-box-containing luciferase reporter construct
in a dose-dependent manner. Conversely, transfection of CT-box oligonucleotide was
able to decrease endogenous N-myc expression in neuroblastoma cells. Together these
results suggest that the CT-box element serves a critical functional role, and in the basal
state allows for N-myc transactivation by Spl and Sp3.
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Filgueira, Pimentel Mirian. "EVALUATION AND FUNCTIONAL CHARACTERIZATION OF BIOCONTROL AGENTS TARGETING SELECT SOILBORNE PATHOGENS OF SOYBEAN". OpenSIUC, 2021. https://opensiuc.lib.siu.edu/dissertations/1886.
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Soybean crops are vulnerable to a wide range of pathogens that reduce yield and cause extensive losses worldwide. In the United States, the soilborne pathogens Pythium spp., causing soybean damping-off, and Fusarium virguliforme, causing sudden death syndrome (SDS) of soybean, have been among the top diseases that most reduced soybean yields. This study demonstrated that biological control using native fungal antagonists could be a powerful tool to integrate with current management strategies for more efficient control of Pythium damping-off and SDS in soybean. Trichoderma spp. and Clonostachys rosea demonstrated the ability to mycoparasitize and antagonize the pathogens using different mechanisms and exhibited a protective effect on soybean in field conditions. The development of an efficient biological control program for disease management relies on a deep understanding of the BCA-pathogen interaction’s biology. This research also uncovered the molecular mechanisms involved in the F. virguliforme-T. afroharzianum interaction by using a dual RNAseq approach. Significant changes in both fungal organisms’ transcriptomes were discovered at different stages in their interaction. The results provided here can contribute to the future implementation of effective biological control programs for soybean. The benefits may also extend to other crops.
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Xie, Yimeng. "Advancements in Degradation Modeling, Uncertainty Quantification and Spatial Variable Selection". Diss., Virginia Tech, 2016. http://hdl.handle.net/10919/71687.
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This dissertation focuses on three research projects: 1) construction of simultaneous prediction intervals/bounds for at least k out of m future observations; 2) semi-parametric degradation model for accelerated destructive degradation test (ADDT) data; and 3) spatial variable selection and application to Lyme disease data in Virginia. Followed by the general introduction in Chapter 1, the rest of the dissertation consists of three main chapters. Chapter 2 presents the construction of two-sided simultaneous prediction intervals (SPIs) or one-sided simultaneous prediction bounds (SPBs) to contain at least k out of m future observations, based on complete or right censored data from (log)-location-scale family of distributions. SPI/SPB calculated by the proposed procedure has exact coverage probability for complete and Type II censored data. In Type I censoring case, it has asymptotically correct coverage probability and reasonably good results for small samples. The proposed procedures can be extended to multiply-censored data or randomly censored data. Chapter 3 focuses on the analysis of ADDT data. We use a general degradation path model with correlated covariance structure to describe ADDT data. Monotone B-splines are used to modeling the underlying degradation process. A likelihood based iterative procedure for parameter estimation is developed. The confidence intervals of parameters are calculated using the nonparametric bootstrap procedure. Both simulated data and real datasets are used to compare the semi-parametric model with the existing parametric models. Chapter 4 studies the Lyme disease emergence in Virginia. The objective is to find important environmental and demographical covariates that are associated with Lyme disease emergence. To address the high-dimentional integral problem in the loglikelihood function, we consider the penalized quasi loglikelihood and the approximated loglikelihood based on Laplace approximation. We impose the adaptive elastic net penalty to obtain sparse estimation of parameters and thus to achieve variable selection of important variables. The proposed methods are investigated in simulation studies. We also apply the proposed methods to Lyme disease data in Virginia. Finally, Chapter 5 contains general conclusions and discussions for future work.Ph. D.
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Arcangeli, Daniele. "Proprietà optoelettroniche di film nanoporosi ottenuti per impiantazione ionica". Bachelor's thesis, Alma Mater Studiorum - Università di Bologna, 2014. http://amslaurea.unibo.it/7702/.
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In questo elaborato vengono studiate le proprietà optoelettroniche di film sottili di germanio nanoporosi ottenuti per impiantazione ionica. Viene trattata la tecnica sperimentale della Surface Photovoltage Spectroscopy nella configurazione Metal-Insulator-Semiconductor. Viene osservato che la presenza di struttura nanoporosa provoca due effetti: il primo è l’innalzamento del bandgap energetico dovuta al confinamento quantistico degli elettroni all’interno dei pori; il secondo è l’incremento del segnale SPV per quei film in cui è maggiore il rapporto tra superficie e volume.
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Rey, N. "Matériaux carbonés sp2/sp3 intercalés sous pression : le cas du graphite et des clathrates". Phd thesis, Université Claude Bernard - Lyon I, 2007. http://tel.archives-ouvertes.fr/tel-00192121.
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L'objectif de ce travail de thèse est d'étudier l'évolution du graphite intercalé avec des atomes alcalins (Li, Rb et Cs) sous haute pression et haute température, procédé susceptible de conduire aux clathrates de carbone, matériaux formés de nano-cages de type sp3 aux propriétés mécaniques proches du diamant et potentiellement supraconducteur à haute température. L'évolution complexe des propriétés structurales et électroniques sous pression des composés CsC8 et RbC8 a été mise en évidence grâce à l'utilisation de sondes locales (spectroscopies d'absoprtion X et Raman) et de sondes à longue distance (diffraction de rayons X et de neutrons). Des nouvelles informations jusqu'à 32 GPa et 16 GPa portant sur le transfert de charge, l'équation d'état, les transitions structurales de CsC8 et RbC8 respectivemement sont apportées. Des calculs ab initio réalisés sur ces clathrates de carbone afin d'étudier leur stabilité ainsi que leurs propriétés sous pression ont permis d'obtenir des indications concernant leur synthèse.
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Rey, Nicolas. "Matériaux carbonés sp2/sp3 intercalés sous pression : le cas du graphite et des clathrates". Lyon 1, 2007. http://tel.archives-ouvertes.fr/docs/00/19/38/84/PDF/tesisNR_2007.pdf.
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L’objectif de ce travail de thèse est d’étudier l’évolution du graphite intercalé avec des atomes alcalins (Li, Rb et Cs) sous haute pression et haute température, procédé susceptible de conduire aux clathrates de carbone, matériaux formés de nano-cages de type sp3 aux propriétés mécaniques proches du diamant et potentiellement supraconducteur à haute température. L’évolution complexe des propriétés structurales et électroniques sous pression des composés CsC8 et RbC8 a été mise en évidence grâce à l’utilisation de sondes locales (spectroscopies d’absoprtion X et Raman) et de sondes à longue distance (diffraction de rayons X et de neutrons). Des nouvelles informations jusqu’à 32 GPa et 16 GPa portant sur le transfert de charge, l’équation d’état, les transitions structurales de CsC8 et RbC8 respectivemement sont apportées. Des calculs ab initio réalisés sur ces clathrates de carbone afin d’étudier leur stabilité ainsi que leurs propriétés sous pression ont permis d’obtenir des indications concernant leur synthèseIn the present work, we have studied the evolution of alkali graphite intercalation compounds (with Li, Rb and Cs) under high pressure and high temperature conditions. This approach could lead to the formation of a cage-like class of materials, the carbon clathrates. Exceptional mechanical properties and elevate superconducting temperature have been predicted in their case. The high-pressure evolution of the structural and electronic properties of CsC8 and RbC8 is discussed. Using both local (X-ray absorption and Raman spectroscopies) and long-range order probes (X-ray and neutron diffraction), new informations up to 32 GPa and 16 GPa on CsC8 and RbC8 respectively are reported concerning charge transfer, equation of state and pressure-induced transitions. Ab initio calculations on carbon clathrates have been also performed to study their stability, their high-pressure properties and to obtain indications concerning their synthesis
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Baravelli, Massimiliano. "Spettroscopia di fototensione superficiale: Principi e applicazioni a materiali semiconduttori". Bachelor's thesis, Alma Mater Studiorum - Università di Bologna, 2014. http://amslaurea.unibo.it/6664/.
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Nel capitolo 1 si riassumono le proprietà fisiche della superficie dei semiconduttori e sono descritti i principi teorici dell' effetto di fototensione superficiale (SPV) con particolare attenzione sullo studio della SPV in film sottili semiconduttori. Nel capitolo 2 si presenta la strumentazione sperimentale per l'analisidella fototensione
superficiale fornendo alcuni esempi di set-up generici e più particolari.
Infine nel capitolo 3 si descrivono alcune applicazioni della
spettroscopia di fototensione superficiale (SPS).
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Nekkanti, Yelha Phani Kumar. "Ruthenium(II) biscarboxylate-Catalyzed C(sp2)-H and C(sp3)-H Functionalizations by Chelation Assistance". Doctoral thesis, Niedersächsische Staats- und Universitätsbibliothek Göttingen, 2016. http://hdl.handle.net/11858/00-1735-0000-002B-7CCA-E.
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Pereira, Mayne de Oliveira. "Caracterização fenotípica e genotípica de Acinetobacter spp. e Pseudomonas aeruginosa produtores de carbapenemases". Universidade de São Paulo, 2017. http://www.teses.usp.br/teses/disponiveis/9/9136/tde-22062017-154029/.
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A resistência bacteriana a antibióticos é um grave e crescente problema de saúde pública de âmbito mundial. O principal, e mais eficiente, mecanismo de resistência aos β-lactâmicos em bacilos Gram-negativos é a produção de β-lactamases, que possuem a capacidade de hidrolisar o anel β-lactâmicos e consequentemente inativar essa classe de antibióticos. Vale ressaltar, que atualmente os antibióticos β-lactâmicos são os mais utilizados clinicamente, particularmente em infecções graves. Dentre as β-lactamases existentes destacam-se as carbapenemases, enzimas capazes de inativar a maioria dos antibióticos β-lactâmicos. Uma grande preocupação é o fato dessas enzimas, em sua maioria, serem codificadas por plasmídeos, o que propicia a disseminação desses genes de resistência; portanto, é de extrema importância a realização de um rápido e efetivo monitoramento da presença de patógenos portadores desses genes de resistência, para que assim se possa prevenir a disseminação desses determinantes. Foram incluídos neste estudo 230 amostras únicas de Acinetobacter e Pseudomonas aeruginosa resistentes a imipenem detectados em pacientes internados em hospitais privados da cidade de São Paulo durante o período de fevereiro a outubro de 2013. As amostras foram avaliadas quanto à hidrólise de imipenem por espectrofotometria, quanto à presença de genes de carbapenemases por PCR e sequenciamento, e quanto à clonalidade por eletroforese em campos pulsados (PFGE) ou ERIC-PCR. Foram realizados ensaios de conjugação, transformação e sequenciamento completo de plasmídeos. Dentre as amostras de Acinetobacter spp. 80% (88) foram capazes de hidrolisar o imipenem. Dentre esses 76,1% (67) foram positivos para blaOXA-51-like, 19,3% (17) foram positivos para blaOXA-72. blaOXA-23, blaOXA-482 e blaIMP-1 foram detectados isoladamente em isolados distintos. O gene blaIMP-1 foi detectado em A. ursingii inserido em integron de classe 1 e representa a primeira descrição no Brasil. Uma nova carbapenemase OXA-482-like foi detectada em A. baumanii. Utilizando-se ERIC-PCR, observou-se uma grande diversidade de grupos clonais, com o máximo de quatro isolados por grupo. Dentre as amostras de P. aeruginosa, apenas 35,3% foram capazes de hidrolisar o imipenem. Dessas amostras, 14 possuíam o gene blaSPM-1, e isolados únicos possuíam, individualmente, os genes blaIMP, blaVIM, blaKPC-2 ou blaGES-23. O gene blaKPC-2 foi detectado inserido em contexto genético diferente dos descritos anteriormente, em plasmídeo IncU de 32 Kb, mobilizável, mas não conjugativo. Esta é a primeira descrição da sequencia completa de plasmídeo albergando o gene blaKPC-2 em P. aeruginosa no Brasil. Nas demais amostras (20) com atividade hidrolítica, não foram detectados genes de carbapenemase conhecidos, o que sugere a presença de genes de carbapenemase ainda não descritos. Em três amostras foi possível obter transformantes com plasmídeos, resistentes a carbapenêmicos. As amostras com blaSPM-1 apresentaram perfis de PFGE estreitamente relacionados. Em contraste, os perfis de PFGE das amostras com potenciais novas carbapenemases apresentaram índice de similaridade de Dice inferior ix a 80%, evidenciando grande diversidade clonal. Nossos achados evidenciam que a carbapenemase não intrínseca predominante em Acinetobacterem hospitais privados da cidade de São Paulo é OXA-72, e em hospitais privados há uma grande diversidade clonal. Em P. aeruginosa, a carbapenemase predominante é SPM-1, cuja disseminação é mediada por um único clone. Há potencialmente um número significativo de novas carbapenemases em Acinetobacter e P. aeruginosa, algumas delas mediadas por plasmídeos.Bacterial resistance to antibiotics is a serious and growing public health problem worldwide. The main and most efficient mechanism of resistance to β-lactams in Gram-negative bacilli is the production of β-lactamases, which have the ability to hydrolyze the β-lactam ring and consequently inactivate this class of antibiotics. It is worth mentioning that currently β-lactam antibiotics are the most used clinically, particularly in severe infections. Among the existing β-lactamases, carbapenemases are capable of inactivating most β-lactam antibiotics. A major concern is that these enzymes are mostly encoded by plasmids, which facilitates the spread of these resistance genes; therefore, it is of extreme importance to carry out a rapid and effective monitoring of the presence of pathogens bearing these resistance genes, in order to prevent the dissemination of these determinants. This study included 230 unique samples of imipenem-resistant Acinetobacterand Pseudomonas aeruginosa detected in patients hospitalized in private hospitals in the city of São Paulo during the period from February to October 2013. The samples were evaluated for the imipenem hydrolysis by spectrophotometry, the presence of carbapenemase genes by PCR and sequencing, and concerning clonality by pulsed field electrophoresis (PFGE) or ERIC-PCR. Conjugation, transformation and complete sequencing of plasmids were performed. Among Acinetobacter spp. samples, 80% (88) were able to hydrolyze imipenem. Among these, 76.1% (67) were positive for blaOXA-51-like genes and 19.3% (17) were positive for blaOXA-72. The blaOXA-23, blaOXA-482 and blaIMP-1 genes were detected alone in distinct isolates. The blaIMP-1 gene was detected in A. ursingii inserted in class 1 integron and represents the first description in Brazil. A novel OXA-482-like carbapenemase was detected in A. baumanii. Using ERIC-PCR, a great diversity of clonal groups was observed, with a maximum of four isolates per group. Among P. aeruginosa samples, only 35.3% were able to hydrolyze imipenem. Of these samples, 14 had the blaSPM-1 gene, and single isolates individually possessed the blaIMP, blaVIM, blaKPC-2 or blaGES-23 genes. The blaKPC-2 gene was found inserted in a genetic context different from those described previously, in a mobilizable, but not conjugative, 32 Kb IncU plasmid. This is the first description of the complete nucleotide sequence of a plasmid harboring the blaKPC-2 gene in P. aeruginosa in Brazil. In the remaining samples (20) with hydrolytic activity, no known carbapenemase genes were detected, suggesting the presence of carbapenemase genes not yet described. In three samples it was possible to obtain transformants with plasmids, resistant to carbapenems. Samples with blaSPM-1 showed closely related PFGE profiles. In contrast, the PFGE profiles of the samples with potential new carbapenemases showed Dice similarity index lower than 80%, evidencing a great clonal diversity. Our findings show that the predominant non-intrinsic carbapenemase in Acinetobacter in the city of São Paulo is OXA-72, and in private hospitals there is great clonal diversity. In P. aeruginosa, the predominant carbapenemase is SPM-1, the spread of this enzyme is mediated by a single clone. There are potentially a significant number of new carbapenemases in Acinetobacter and P. aeruginosa, some of them plasmid mediated.
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Haffemayer, Benjamin. "Development of novel amine-directed Pd(II)-catalysed sp2 and sp3 C-H bond functionalisation processes". Thesis, University of Cambridge, 2013. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.607792.
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Weber, J??rgen Wolfgang Photovoltaic & Renewable Engergy Engineering UNSW. "Design, construction and testing of a high-vacuum anneal chamber for in-situ crystallisation of silicon thin-film solar cells". Awarded by:University of New South Wales. Photovoltaic and Renewable Engergy Engineering, 2006. http://handle.unsw.edu.au/1959.4/24847.
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Thin-film solar cells on glass substrates are likely to have a bright future due to the potentially low costs and the short energy payback times. Polycrystalline silicon (poly-Si, grain size > 1 pm) has the advantage of being non-toxic, abundant, and long-term stable. Glass as a substrate, however, limits the processing temperatures to ~600??C for longer process steps. Films with large grain size can be achieved by solid phase crystallisation (SPC), and especially by solid phase epitaxy (SPE) on seed layers, using amorphous silicon deposited at low temperatures as a precursor film. With SPC and SPE, the amorphous silicon film is typically crystallised at ~600??C over hours. During this anneal at atmospheric pressure -depending on the properties of the amorphous silicon film- ambient gas can percolate the film and can negatively affect the crystallisation. In this work, a high-vacuum anneal chamber was designed and built to allow the in-situ crystallisation of amorphous silicon films deposited on glass in a PECVD cluster tool. An important aspect of the design was the comfortable and safe operation of the vacuum anneal chamber to enable unattended operation. This was realised by means of a state-of-the-art, programmable temperature controller and a control circuit design that incorporates various safety interlocks. The chamber interior was optimised such that a temperature uniformity of 2-3K across the sample area was achieved. The chamber was calibrated and tested, and SPC and SPE samples were successfully crystallised. In initial SPC crystallisation experiments with solar cell structures, after post-deposition treatments, a 1 -sun open-circuit voltage of 465 mV was obtained, similar to furnace-annealed samples. In initial experiments with SPE solar cell structures, difficulties regarding the characterisation of the unmetallised solar cells with the quasi-steady-state open-circuit voltage method (QSSVOC) were encountered after post-deposition hydrogen treatment. A possible explanation for these difficulties is the contact formation with the metal probes. Furthermore, limiting factors of the QSSVOC method for the characterisation of unmetallised cells with high contact resistance values were investigated and, additionally, the accuracyof the QSSVOC setup was improved in the low light intensity range.
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Kajita, Yoichiro. "The transcription factor Sp3 regulates the expression of a metastasis-related marker of sarcoma, actin filament-associated protein 1-like 1(AFAP1L1)". Kyoto University, 2013. http://hdl.handle.net/2433/179345.
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Huang, Mei-hui. "Factors influencing self-directed learning readiness amongst Taiwanese nursing students". Thesis, Queensland University of Technology, 2008. https://eprints.qut.edu.au/20709/1/Mei-hui_Huang_Thesis.pdf.
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Rapid scientific and technological advances in health care mean that nurses need to keep learning and engage in professional education so that they can continue to provide safe and quality care. Education programs which prepare nurses for practice as a registered nurse have a vital role to play in ensuring that graduates are self-directed in responding to the need for ongoing learning throughout their professional career. In many countries, improving students’ readiness for self-directed learning has thus gained increasing recognition as being an important goal of nursing education programs. This level of interest in developing self-directedness in learning is evident in many policy documents and research in Taiwan. The aim of this study was to investigate factors influencing self-directed learning readiness amongst Taiwanese nursing students. A conceptual framework adopted from Biggs’s ‘3P model of teaching and learning’ was constructed to guide this study’s investigation. This study employed a two-staged mixed-method design to obtain a better understanding of Taiwanese students’ experience of SDL in undergraduate nursing programs. Stage one of the present study was a qualitative approach using semi-structured interview to explore students’ experiences with learning activities which they perceived to be self-directed in their undergraduate programs. Eight students were interviewed. Findings from this stage reveal that participants perceived a shift in teaching and learning styles between their previous nursing programs and the university. The more frequent use of student-directed learning activities, in which students were encouraged to be active and to take responsibility for their learning tasks, was one of the changes in teaching and learning approaches perceived by participants. Participants further suggested a number of factors that influenced the outcomes of these learning activities, including teacher-student interaction, facilitation process and learning resources. Stage two of this study used a quantitative approach consisting of two phases: instrument pilot testing and a cross-sectional survey. In the first phase, the instruments were translated into Chinese through a rigorous translation process and tested with a convenience sample of nursing students in Taiwan. Results indicated the translated instruments were reliable and stable. The second phase, a cross-sectional survey, was conducted to examine the conceptual framework of this study. A total of 369 undergraduate nursing students completed the questionnaire. Results of data analysis provides support for the conceptual framework proposed for this study, suggesting that students’ achievement goals and their perceptions of the learning environment significantly influence their adoption of learning approaches and the development of SDL readiness. Based on the results, this study provides practical implications that nurse educators may adopt to enhance students’ SDL readiness. This study also provides theoretical implications and recommendations for future research. It is envisaged that these recommendations may help future researchers focus their research design and further understandings of how to help students develop their ability to become self-directed learners.
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Huang, Mei-hui. "Factors influencing self-directed learning readiness amongst Taiwanese nursing students". Queensland University of Technology, 2008. http://eprints.qut.edu.au/20709/.
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Rapid scientific and technological advances in health care mean that nurses need to keep learning and engage in professional education so that they can continue to provide safe and quality care. Education programs which prepare nurses for practice as a registered nurse have a vital role to play in ensuring that graduates are self-directed in responding to the need for ongoing learning throughout their professional career. In many countries, improving students’ readiness for self-directed learning has thus gained increasing recognition as being an important goal of nursing education programs. This level of interest in developing self-directedness in learning is evident in many policy documents and research in Taiwan. The aim of this study was to investigate factors influencing self-directed learning readiness amongst Taiwanese nursing students. A conceptual framework adopted from Biggs’s ‘3P model of teaching and learning’ was constructed to guide this study’s investigation. This study employed a two-staged mixed-method design to obtain a better understanding of Taiwanese students’ experience of SDL in undergraduate nursing programs. Stage one of the present study was a qualitative approach using semi-structured interview to explore students’ experiences with learning activities which they perceived to be self-directed in their undergraduate programs. Eight students were interviewed. Findings from this stage reveal that participants perceived a shift in teaching and learning styles between their previous nursing programs and the university. The more frequent use of student-directed learning activities, in which students were encouraged to be active and to take responsibility for their learning tasks, was one of the changes in teaching and learning approaches perceived by participants. Participants further suggested a number of factors that influenced the outcomes of these learning activities, including teacher-student interaction, facilitation process and learning resources. Stage two of this study used a quantitative approach consisting of two phases: instrument pilot testing and a cross-sectional survey. In the first phase, the instruments were translated into Chinese through a rigorous translation process and tested with a convenience sample of nursing students in Taiwan. Results indicated the translated instruments were reliable and stable. The second phase, a cross-sectional survey, was conducted to examine the conceptual framework of this study. A total of 369 undergraduate nursing students completed the questionnaire. Results of data analysis provides support for the conceptual framework proposed for this study, suggesting that students’ achievement goals and their perceptions of the learning environment significantly influence their adoption of learning approaches and the development of SDL readiness. Based on the results, this study provides practical implications that nurse educators may adopt to enhance students’ SDL readiness. This study also provides theoretical implications and recommendations for future research. It is envisaged that these recommendations may help future researchers focus their research design and further understandings of how to help students develop their ability to become self-directed learners.
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Rožanskė, Rūta. "SPA paslaugų kokybės vertinimas „HARMONY SPA“". Bachelor's thesis, Lithuanian Academic Libraries Network (LABT), 2013. http://vddb.laba.lt/obj/LT-eLABa-0001:E.02~2013~D_20130906_105248-13960.
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Objektas – paslaugų kokybė „Harmony SPA“.
Darbo tikslas – įvertinti SPA paslaugų kokybę vartotojų požiūriu.
Darbo uždaviniai:
1. Atskleisti paslaugų kokybės sampratą;
2. Atskleisti SPA paslaugų kokybės ypatumus;
3. Ištirti SPA paslaugų kokybės lygį organizacijoje „Harmony SPA“
Išvados:
1. Paslaugų kokybė - vartotojų paslaugų vertės suvokimas, tai skirtumas tarp vartotojų lūkesčių ir patirtos kokybės, kurią labai sunku išmatuoti, įvertinti ar apskaičiuoti. Paslaugų kokybė pasižymi šiais požymiais : patikimumas, jautrumas, įtikinamumas, pritarimas, išorinis akivaizdumas. Paslauga - skirtingai nei prekė, yra ne daiktas, o vyksmas (veiksmas ar jų aibė); proceso ir rezultato sintezė, nes yra neapčiuopiamas veiklos ir prekės derinys. Paslaugos kaip ir prekės pasižymi tam tikromis savybėmis.. Paslaugos terminas vartojamas ekonominės veiklos šakai apibūdinti, veiklos rezultatui nustatyti ir apibrėžti procesui, kai teikiant paslaugą vyksta asmenų sąveika ir vartotojas įgauna laikiną patyrimą.
2. SPA paslauga - vandens procedūrų (SPA) paslaugos – kvalifikuotų specialistų specialiai įrengtose patalpose atliekamos ir (ar) prižiūrimos procedūros, kurioms naudojamas vanduo ir kurios skiriamos fizinei ir psichikos sveikatai gerinti, gerai savijautai skatinti. SPA paslaugų kokybės ypatumai - prieinamumas finansine ir geografine prasme, įmonės resursai, personalo elgesys paslaugos teikimo metu, gebėjimas vertinti vartotojų reikmes, juntami pokyčiai po paslaugos, įvertinimas tik po tam... [toliau žr. visą tekstą]SUMMARY Keywords : SPA, facilities, consumer, quality. Object – service quality „Harmony SPA“ . The aim – evaluate SPA service quality at the point of consumer‘s. Work tasks : 1. To reveal service quality term. 2. To reveal SPA services quality peculiarity. 3. To investigate SPA services quality level in organisation „Harmony SPA“ Conclusions 1. Service quality – consumer perception of service value, difference of consumer expectations and quality of the experience. Service quality characterictics : reliability, sensitivity, stringency, approval, the external evidence. Service – different from goods, it is not a thing, it is a process and the sequence of actions; synthesis of process and result, because it is not intangible, it is a combination of action and merchandise. It is important to know that services are appropriate for the customer or it need to be improved and how to do it, so we need to evaluate service. 2. SPA service – water treatments (SPA) services – provided procedures of qualified specialists, in a specially equipped room, which is used water and which is for physical and psychical health to improve, well-being to promote. The term of service is used to describe branches of economical activity, results of activity and proces to define. 3. Compared results by all five SPA services quality indicative quality dimensions (tangibility, reliability, certainty, empathy, sensitivity) it can be said that the organisation provides extremely high level of SPA... [to full text]
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Cofre, Teresa Del Carmen Gabriel [UNESP]. "Transcriptoma (RNA-seq) de laranja doce Valência (Citrus sinensis L. Osbeck) e Kumquat (Fortunella spp.) infectadas por Xanthomonas citri subsp. citri". Universidade Estadual Paulista (UNESP), 2013. http://hdl.handle.net/11449/92721.
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000722118.pdf: 1821985 bytes, checksum: 5390d9268bd2f6af7699b7a2497c2f5c (MD5)Dentre as diversas doenças que ocorrem na citricultura, o cancro cítrico é uma das principais, considerada economicamente importante e com impactos diretos e indiretos no setor citrícola mundial. O agente causal do cancro cítrico é a bactéria Xanthomonas citri subsp. citri (Xac), a qual afeta uma ampla gama de espécies e variedades de citros. A variedade de laranja doce Valência (Citrus sinensis) é considerada moderadamente suscetível ao cancro cítrico e a Rutaceae Kumquat (Fortunella spp.), por outro lado, é considerada altamente resistente. Objetivando gerar informações acerca dos mecanismos moleculares que resultam em diferenças quanto à suscetibilidade de plantas a patógenos, no presente estudo fez-se uso de uma nova plataforma de sequenciamento de transcriptoma (RNA-seq) para análise da expressão gênica de Valência e Kumquat após infecção por Xac nos tempos de 24, 48 e 72 horas. Foram identificados 2.953 e 4.111 transcritos induzidos em Valência e Kumquat, respectivamente. Dos transcritos reprimidos Kumquat apresentou 11.041 e Valência 1.930. Na comparação da expressão gênica temporal, Kumquat apresentou muitos transcritos induzidos em comum em todos os tempos de infecção, alguns deles relacionados à percepção de sinais (receptores do tipo quinase) e também envolvidos na defesa de plantas [(como proteínas relacionadas à patogênese (PRs)]. Foram identificados exclusivamente em Kumquat transcritos que codificam para a proteína germin, possivelmente envolvida na resposta de defesa à Xac. Nas comparações entre espécies, em Kumquat observou-se alta expressão de proteínas relacionadas à síntese de metabólitos secundários, como alcaloides e monoterpenos. Em Valência, nos tempos iniciais de 24 e 48 horas após a inoculação de Xac, observou-se alta expressão de cisteína proteinases, as quais comumente...
Among the various diseases that occur in Citrus spp., citrus canker is a serious disease, considered economically important with direct impact on the citrus sector. The causal agent of citrus canker is the bacterium Xanthomonas citri subsp. citri (Xac), and it affects a wide range of Citrus spp.. Sweet orange Valencia Citrus sinensis is considered moderately susceptible to Citrus canker and the Rutaceae Kumquat Fortunella spp. is considered highly resistant. To understand the molecular mechanisms that distinguish plant resistance in plant pathogen interaction, the present study used a new transcriptome sequencing platform (RNA-seq) to analyze gene expression of Valencia and Kumquat after infection by Xac post 24, 48 and 72 hours. We identified 2,953 and 4,111 transcripts induced in Valencia and Kumquat, respectively. Among repressed transcripts Kumquat showed 11,041 and Valencia 1,930. In the comparison of temporal expression between the evaluated times, Kumquat presented many transcripts induced in common at all times of infection that are related to signal perception, as receptors kinase and plant defense proteins as pathogenesis related proteins (PRs). Were found exclusively in Kumquat transcripts that encode for protein Germin possibly involved in defense response to Xac. In the analysis between species, for Kumquat were found a high expression of proteins related to the synthesis of secondary metabolites, such as alkaloids and monoterpenes. These products may be mediated by biotic factors such as pathogen attack, so the high expression of genes related to metabolic production of these can be related to plant defense. Valencia presented at 24 and 48 hours high expression of cysteine proteinases. These are involved in reconstruction and degradation of proteins in response to different stimuli and also... (Complete abstract click electronic access below)
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Cofre, Teresa Del Carmen Gabriel. "Transcriptoma (RNA-seq) de laranja doce Valência (Citrus sinensis L. Osbeck) e Kumquat (Fortunella spp.) infectadas por Xanthomonas citri subsp. citri /". Araraquara, 2013. http://hdl.handle.net/11449/92721.
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Orientador: Henrique FerreiraCoorientador: José Belasque Junior
Banca: Jesus Aparecido Ferro
Banca: Nelson Arno Wulff
Resumo: Dentre as diversas doenças que ocorrem na citricultura, o cancro cítrico é uma das principais, considerada economicamente importante e com impactos diretos e indiretos no setor citrícola mundial. O agente causal do cancro cítrico é a bactéria Xanthomonas citri subsp. citri (Xac), a qual afeta uma ampla gama de espécies e variedades de citros. A variedade de laranja doce Valência (Citrus sinensis) é considerada moderadamente suscetível ao cancro cítrico e a Rutaceae Kumquat (Fortunella spp.), por outro lado, é considerada altamente resistente. Objetivando gerar informações acerca dos mecanismos moleculares que resultam em diferenças quanto à suscetibilidade de plantas a patógenos, no presente estudo fez-se uso de uma nova plataforma de sequenciamento de transcriptoma (RNA-seq) para análise da expressão gênica de Valência e Kumquat após infecção por Xac nos tempos de 24, 48 e 72 horas. Foram identificados 2.953 e 4.111 transcritos induzidos em Valência e Kumquat, respectivamente. Dos transcritos reprimidos Kumquat apresentou 11.041 e Valência 1.930. Na comparação da expressão gênica temporal, Kumquat apresentou muitos transcritos induzidos em comum em todos os tempos de infecção, alguns deles relacionados à percepção de sinais (receptores do tipo quinase) e também envolvidos na defesa de plantas [(como proteínas relacionadas à patogênese (PRs)]. Foram identificados exclusivamente em Kumquat transcritos que codificam para a proteína germin, possivelmente envolvida na resposta de defesa à Xac. Nas comparações entre espécies, em Kumquat observou-se alta expressão de proteínas relacionadas à síntese de metabólitos secundários, como alcaloides e monoterpenos. Em Valência, nos tempos iniciais de 24 e 48 horas após a inoculação de Xac, observou-se alta expressão de cisteína proteinases, as quais comumente... (Resumo completo, clicar acesso eletrônico abaixo)
Abstract: Among the various diseases that occur in Citrus spp., citrus canker is a serious disease, considered economically important with direct impact on the citrus sector. The causal agent of citrus canker is the bacterium Xanthomonas citri subsp. citri (Xac), and it affects a wide range of Citrus spp.. Sweet orange Valencia Citrus sinensis is considered moderately susceptible to Citrus canker and the Rutaceae Kumquat Fortunella spp. is considered highly resistant. To understand the molecular mechanisms that distinguish plant resistance in plant pathogen interaction, the present study used a new transcriptome sequencing platform (RNA-seq) to analyze gene expression of Valencia and Kumquat after infection by Xac post 24, 48 and 72 hours. We identified 2,953 and 4,111 transcripts induced in Valencia and Kumquat, respectively. Among repressed transcripts Kumquat showed 11,041 and Valencia 1,930. In the comparison of temporal expression between the evaluated times, Kumquat presented many transcripts induced in common at all times of infection that are related to signal perception, as receptors kinase and plant defense proteins as pathogenesis related proteins (PRs). Were found exclusively in Kumquat transcripts that encode for protein Germin possibly involved in defense response to Xac. In the analysis between species, for Kumquat were found a high expression of proteins related to the synthesis of secondary metabolites, such as alkaloids and monoterpenes. These products may be mediated by biotic factors such as pathogen attack, so the high expression of genes related to metabolic production of these can be related to plant defense. Valencia presented at 24 and 48 hours high expression of cysteine proteinases. These are involved in reconstruction and degradation of proteins in response to different stimuli and also... (Complete abstract click electronic access below)
Mestre
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Allegretti, Luciana. "Isolamento e identificação de Lactobacillus spp., Bifidobacterium spp., Enterococcus spp., Pediococcus spp. e Lactococcus spp. da microbiota intestinal de Papagaio-verdadeiro (Amazona aestiva)". Universidade de São Paulo, 2009. http://www.teses.usp.br/teses/disponiveis/10/10133/tde-08122009-143059/.
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No Brasil, o papagaio-verdadeiro (Amazona aestiva) é uma das aves mais procuradas como animal de estimação e comercializadas ilegalmente. Na literatura pouco é descrito sobre a microbiota intestinal de aves silvestres. O trato intestinal das aves é composto por inúmeras e diferentes espécies bacterianas. A grande maioria são bactérias gram-positivas pertencentes ao grupo de bactérias ácido-láticas. Este estudo teve como objetivo isolar e identificar a presença de bactérias dos gêneros Lactobacillus, Bifidobacterium, Enterococcus, Pediococcus e Lactococcus na microbiota entérica de papagaios Amazona aestiva de vida livre e de cativeiro. Para isto foram coletadas amostras de 26 aves de vida livre e de 26 aves procedentes de dois criadouros comerciais. O Enterococcus foi o gênero que apresentou maior freqüência de isolamentos (100%), seguido dos gêneros Pediococcus (63,46%), Lactobacillus (28,84%), Lactococcus e Bifidobacterium (15,38%). Foram isoladas 12 espécies de Enterococcus, sendo o E. faecium a espécie que apresentou maior ocorrência de isolamento, presente em 63,46% das aves, seguido por E. faecalis isolado em 57,69% das aves, Enterococcus sp. identificado em 46,15% das aves, E. hirae em 30,76% e E. raffinosus em 19,23%. Seis espécies de Pediococcus foram isoladas, sendo que P. pentosaceus foi a mais freqüente e esteve presente em 57,69% das aves. Foram isoladas cinco (5) espécies de Lactococcus, sendo L. lactis subsp. cremoris isolados em 3,84% das aves e Lactococcus sp. em 9,61%. Lactobacillus apresentou uma maior diversidade, com 14 espécies identificadas, sendo as mais freqüentes L. coryniformis subsp. torquens e L. sanfrancisco com 7,69% de aves positivas para cada espécie. Três (3) espécies de Bifidobacterium foram isoladas, sendo B. bifidum identificado em 9,61% das aves. Estudos complementares precisam ser conduzidos para uma melhor compreensão da microbiota intestinal das aves silvestres, assim como analisar as similaridades e diferenças com as aves domésticas, o que permitirá um manejo apropriado e menos empírico desta espécie em cativeiro.In Brazil, Blue-fronted Parrot (Amazona aestiva) has been widely owned as a pet bird and, therefore, one of the Brazilians birds most frequently traded illegally in the Black Market. There are few reports in the current literature regarding to the microbiota of wild birds. The gastrointestinal tract of these birds has a wide variety of bacterial species; most of them are Gram positive bacteria and belongs to the lactic acid group. The present study has isolated and identified Lactobacillus, Bifidobacterium, Enterococcus, Pediococcus, and Lactococcus bacterias present in fecal samples of wild and captive Amazona aestiva parrots. Fifty two fecal samples were collected from 26 wild parrots and 26 parrots from commercial breeders. Enterococcus genus was the most frequently isolated (100%), followed by Pediococcus (63.46%), Lactobacillus (28.84%), Lactococcus and Bifidobacterium (15.38%). Twelve species of Enterococcus were identified. E. faecium was the most frequently isolated from the birds representing 63.46%, followed by E. faecalis (57.69%), Enterococcus sp. (46.15%), E. hirae (30.76%), and E. raffinosus (19.23%). P. pentosaceus was identified from 57.69% of the parrots. This specie was the most frequently isolated. Five different species of Lactococcus were found out. Lactococcus sp. was identified from 9.61% of the birds, while L. lactis subsp. lactis represented 3.84%. Fourteen different species of Lactobacillus were isolated, showing the biggest diversity among all the studied genera. L. coryniformis subsp. torquens and L. sanfrancisco were isolated from 7.69% of the birds. Three different species of Bifidobacterium were isolated, and B. bifidum was identified in 9.61% of the birds, being the most frequently isolated. Further studies are needed to a better comprehension of the microbiota in wild birds. Besides comparing differences and similarities between wildlife parrots and pet birds will allow appropriate and less empiric management of those birds in captivity.
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Barreto, J?ssica Pires de Paiva. "Estudo da rela??o sp3/sp2 de ?nodos de diamante dopado com boro (BDD) na produ??o de esp?cies fortemente oxidantes". PROGRAMA DE P?S-GRADUA??O EM QU?MICA, 2016. https://repositorio.ufrn.br/jspui/handle/123456789/22255.
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Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior (CAPES)
A possibilidade de preparar diamante sint?tico aumentou o interesse da comunidade cient?fica para aplica??o destes materiais, devido as suas caracter?sticas peculiares. Entretanto, a dopagem do diamante sint?tico com N, P, B e F torna-o semicondutor podendo ser aplicado na eletroqu?mica. No decorrer das pesquisas ficou estabelecido que o diamante dopado com boro, BDD (sigla em ingl?s) ? um ?nodo que n?o favorece a adsor??o qu?mica das esp?cies na sua superf?cie. Por?m, recentemente a influ?ncia das caracter?sticas do filme (rugosidade, espessura, teor de boro e impurezas sp2) foi determinada; e estes par?metros podem afetar a efic?cia do ?nodo na produ??o de oxidantes fortes e na degrada??o de compostos org?nicos. Por estas raz?es, estudar a influ?ncia da raz?o sp3/sp2 fornece informa??es sobre a escolha de um ?nodo espec?fico de BDD para aplica??es eletroqu?micas. Este trabalho tem como objetivos comparar ?nodos de BDD com diferente raz?o sp3/sp2 para avaliar o desempenho na produ??o de persulfato aplicando diferentes densidades de corrente, bem como, abordar ? atua??o dessa esp?cie fortemente oxidante na degrada??o de um composto modelo, Acid Violet 7. Al?m disso, o desenvolvimento de um estudo toxicol?gico empregando Lactuca Sativa atrav?s da efici?ncia de descontamina??o do efluente sint?tico mediante persulfato eletroquimicamente gerado. Os resultados claramente demonstram que o eletrodo de BDD, contendo maior teor de grafite e maior propriedade de adsor??o na superf?cie, favorece a produ??o significativa do oxidante de interesse. O melhor tempo de eletr?lise para a produ??o m?xima/ideal da esp?cie foi de 120 minutos. O tratamento eletroqu?mico para o Acid Violet 7 usando eletrodos de BDD com diferente raz?o sp3/sp2 (175 e 329) mostrou que, para ambos os casos, a elimina??o da cor e a degrada??o da mat?ria org?nica foram atingidas, por?m um maior n?vel de mineraliza??o foi observado com o BDD 329, indicando uma melhor descontamina??o do efluente. Ap?s o tratamento eletroqu?mico, an?lises toxicol?gicas comprovaram que o efluente tratado com o BDD 175 permitiu a germina??o das sementes de Lactuca Sativa. Por?m, o resultado obtido ? contr?rio ao grau de mineraliza??o obtido com o BDD 329. Esse comportamento foi devido ? significativa produ??o de oxidantes e de ?cidos alif?ticos no final do tratamento eletroqu?mico com BDD 329, evitando a germina??o de Lactuca Sativa.
The possibility of preparing synthetic diamond has increased the interest of the scientific community for application of these materials due to their unique characteristics. However, the synthetic diamond films doping with N, P, B and F become the semiconductor for application in electrochemistry. During the research it was established that the boron-doped diamond, BDD is an anode that does not favor the chemical adsorption of the species on its surface. Recently, however, the influence of film characteristics (surface roughness, thickness, and impurities boron content sp2) was determined; and these parameters can affect the effectiveness of the anode in the production of strong oxidants and degradation of organic compounds. For these reasons, study the influence of ratio sp3/sp2 provides information about choosing a specific anode BDD for specific electrochemical applications. Thus, this work has as main objectives to compare anodes BDD with different ratio sp3/sp2 to evaluate performance in the production of persulfate by applying different current densities, as well as addressing the role that strongly oxidizing species during the degradation of a model compound, Acid Violet 7. Furthermore, the development of a toxicology study using Lactuca sativa through the decontamination efficiency of the synthetic effluent using electrochemically generated persulfate was performed. The results clearly show that BDD electrode containing higher graphite content and higher adsorption property on the surface favors the significant production of the oxidant of interest. The best electrolysis time for high production of persulfate was 120 minutes. The electrochemical treatment of the Acid Violet 7 using BDD electrodes with different sp3/sp2 ratio (175 and 329) showed that for both cases, the elimination of color and degradation of organic matter were affected for this parameter. However, a significant mineralization was observed with BDD 329 than that obtained with BDD 175, indicating a better decontamination of effluent. After the electrochemical treatment, the toxicological analyzes showed that the effluent treated with BDD 175 allowed the germination of Lactuca sativa. However, the result is opposite to the degree of mineralization obtained with BDD 329. This behavior was due to the production of significant concentrations of oxidizers and aliphatic acids at the end of electrochemical treatment with BDD 329, preventing seed germination Lactuca sativa.
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VIEIRA, F. T. "OCORRÊNCIA de Ehrlichia Spp., Anaplasma Spp., Babesia Spp., Hepatozoon Spp. e Rickettsia Spp. em Cães Domiciliados em Seis Municípios do Estado do Espírito Santo, Brasil". Universidade Federal do Espírito Santo, 2017. http://repositorio.ufes.br/handle/10/6932.
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Previous issue date: 2017-07-20Doenças transmitidas por carrapatos representam importante causa de morbidade e mortalidade em cães (Canis lupus familiaris) em todo o mundo, tendo o Rhipicephalus sanguineus sensu lato (s.l.) como vetor de vários agentes. É muito importante conhecer a prevalência destas doenças, seus vetores e hospedeiros com o objetivo de controlar e prevenilas em determinada região. Doenças transmitidas por carrapatos apresentam importância tanto para a Medicina Veterinária quanto para a Saúde Pública. Este trabalho tem como objetivo identificar e quantificar o percentual de infecção para Rickettsia spp., Ehrlichia spp., Babesia spp. e Hepatozoon spp. em cães domiciliados e identificar os carrapatos coletados nestes animais nos municípios de Santa Teresa, Colatina, Alegre, Vila Velha, Vitória e Serra. Participaram deste estudo 378 cães, 226 fêmeas e 152 machos, de diversas raças e idades (média de 4,1 anos) com ectoparasitismo (carrapatos) ou histórico de parasitismo recente (até 30 dias). Dos 378 cães examinados, 157 (41,53%) apresentavam carrapatos no momento da consulta os quais foram identificados como R. sanguineus s.l. em 154 animais (98,1%), Amblyomma ovale em um animal (0,63%) e Amblyomma sculptum em 2 animais (1,27%). Todos os animais examinados tiveram sangue total coletados para a realização de exames moleculares: PCR Real Time para Ehrlichia canis, PCR para Babesia spp., PCR para Hepatozoon spp. e PCR para Anaplasmataceae. No PCR Real Time específica para E. canis, 28 animais (7,4%) foram positivos. Para Anaplasmataceae, 34 animais (9%) foram positivos, dos quais 10 geraram sequências de DNA com 100% de similaridade com Ehrlichia canis e as outras 24 amostras geraram um fragmento com 100% de similaridade com Anaplasma platys. No PCR para Babesia spp., 5 animais (1,3%) foram positivos, gerando sequências de DNA 100% idênticas com Babesia vogeli. Por fim, 39 animais (10,31%) foram positivos na PCR para Hepatozoon spp., gerando sequências 100% idênticas a Hepatozoon canis. Observou-se co-infecção com 2 ou 3 agentes em 22 animais (5,8%). Dos 378 cães do estudo, 312 tiveram amostras de soro sanguíneo coletados para a realização de exames sorológicos: reação de imunofluorescência indireta (RIFI) para E. canis, com reação positiva em 71 animais (22,75%) e para cinco espécies de Rickettsia (R. rickettsii, R. parkeri, R. amblyommatis, R. rhipicephali e R. bellii). Dos soros analisados por RIFI,16 animais (5,13%) apresentaram títulos ≥ 64 para pelo menos uma espécie de Rickettsia das cinco testadas, sendo que 5 (1,6%) destes apresentaram títulos ≥ 64 para R. rickettsii. Estes resultados indicam que alguns dos municípios estudados apresentam agentes transmitidos por carrapatos circulando na população canina, tais como E. canis, A. platys, B. vogeli, H. canis, e possivelmente Rickettsia sp. do grupo da febre maculosa.
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Canhoto, Rita Isabel Pires. "Estudo dos fatores de risco associados à infeção por Anaplasma spp., Ehrlichia spp., Babesia spp., Leishmania spp. e Dirofilaria spp. em cães do Alentejo Norte, Portugal". Master's thesis, Universidade de Lisboa, Faculdade de Medicina Veterinária, 2020. http://hdl.handle.net/10400.5/20200.
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Dissertação de Mestrado Integrado em Medicina VeterináriaAs doenças transmitidas por vetores (DTV) são provocadas por vírus, bactérias ou parasitas transmitidos através da fixação ou picada de um artrópode hematófago, sendo as carraças, flebótomos e mosquitos os vetores mais frequentes. São uma preocupação crescente pela sua atual tendência emergente, sendo que o seu carácter zoonótico as coloca como um assunto de One Health. Frequentemente sem manifestações clínicas ou revelando um quadro clínico abrangente e inespecífico, o diagnóstico de infeções por estes agentes é um verdadeiro desafio, prevendo-se que a prevalência destes agentes esteja, muitas vezes, subdiagnosticada. No entanto, esta deteção é fundamental, pois, se por um lado os cães doentes necessitam correto diagnóstico e tratamento, por outro, os cães sem sinais clínicos atuam como sentinelas e hospedeiros reservatório destes agentes, perpetuando a sua transmissão para outros animais e para o Homem. O presente estudo foi elaborado com o objetivo de diagnosticar a prevalência de Anaplasma phagocytophilum, Ehrlichia canis, Babesia canis, Leishmania infantum e Dirofilaria immitis em 102 cães que habitam na região interior norte do Alentejo, determinando quais os fatores de risco associados à infeção por cada um destes agentes. Na observação dos esfregaços sanguíneos não foi possível identificar nenhum destes agentes (0%). Na análise sorológica, através de ELISA, foi diagnosticada uma seroprevalência de A. phagocytophilum de 0% (0/102), uma seroprevalência de E. canis de 22,9% (24/102), uma seroprevalênica de L. infantum de 33,7% (35/102) e uma seroprevalência de D. immitis de 0,98% (1/102). Através de IFI, foi diagnosticada uma seroprevalência de B. canis de 26,5% (27/102). Foram ainda analisados diversos fatores (características individuais, hábitos de vida e profilaxia dos cães amostrados), estudando a sua influência na ocorrência de infeção por cada um destes agentes. Foi determinada uma associação estatística significativa entre animais séniores e animais de raça indefinida e a positividade a E. canis; entre animais séniores e a positividade a B. canis; e, por fim, entre animais de criação, animais com acesso permanente ao exterior e animais que utilizam produtos com ação contra os mosquitos/flebótomos e a positividade a L. infantum.
ABSTRACT - Study of risk factors associated with infection of Anaplasma spp., Ehrlichia spp., Babesia spp., Leishmania spp. and Dirofilaria spp. in dogs from the North Alentejo – Portugal - Vector-borne diseases (VBD) are caused by viruses, bacteria or parasites transmitted by an hematophagous arthropod. Ticks and mosquitoes are the most frequent vectors of pathogens. These diseases are in growing concern for their current emerging trend, and their zoonotic trend makes them a One Health topic. Often in asymptomatic cases or, with a nonspecific clinical picture, the diagnosis of the infections agents is a real challenge, and it is expected that the prevalence rate of these agents is often underestimated. However, this detection is essential, in order to achieve the correct diagnosis and treatment to diseased animals, knowing that asymptomatic dogs act as sentinels and reservoir hosts for these agents, spreading their infection to other animals and to humans. In the present study we studied the prevalence rate of A. phagocytophilum, E. canis, B. canis, L. infantum and D. immitis in 102 dogs that live in North Alentejo, identifying risk factors associated with the infection of these agents. In the observation of blood smears, it was not possible to identify any of these agents (0%). In the serological analysis, by ELISA, A. phagocytophilum seroprevalence was 0% (0/102), E. canis seroprevalence was 22,9% (24/102) and D. immitis seroprevalence was 0,98% (1/102). By IFI, B. canis seroprevalence was 26.5% (27/102) and L. infantum seroprevalence was 33,7% using ELISA (35/102). Several factors were also analyzed (individual characteristics, life habits and prophylaxy routine), studying their influence on the occurrence of infection by each of these agents. A statistically significant association was found between senior animals and animals of non-defined breed and positivity to E. canis; between senior animals and positivity to B. canis; between breeding animals, animals with permanent outdoor lifestyle and animals that use products with action against mosquitoes/sandflies and positivity to L. infantum.
N/A
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Toscan, Gustavo. "Parâmetros imunoparasitológicos de cordeiros naturalmente infectados por Haemonchus spp., Teladorsagia spp. e Trichostrongylus spp". Universidade Federal de Santa Maria, 2016. http://repositorio.ufsm.br/handle/1/4133.
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In the context of the current sheep-rearing, control of parasitic infection establishes a constant challenge to producers, because the gastrointestinal parasites have high prevalence in flocks, highly pathogenic and frequents resistance to anthelmintic products. The parasitic infections of sheep are characterized by simultaneously occur for several genera of nematodes, however, Haemonchus contortus is considered the major gastrointestinal parasites of sheep. In some situations, integrated methods of parasite control available become ineffective in the face of critical situation, aggravated by the parasite resistance to anthelmintics. Therefore, animal immune response became a central point in the dynamics and limiting the consequences related to parasitic infection. The systemic and local immune responses comprise a set of restrictive mechanisms or not characterized according to the animal's age, physiological condition, nutritional and management status. However, precepts of which the immune system works and how occurs is not fully understood, there are some factors to be revealed so that we can establish a secure and lasting immune protection against gastrointestinal parasites. In Chapter 1, shows the characteristics of the immune response of sheep naturally infected with gastrointestinal nematodes kept on native pasture. The study involved a set of immunological, parasitological and clinical techniques to characterize and establish the pathways of the immune response in resilient animals. In this study, was possible to establish a weak negative correlation between Eos and OPG in resilient Corriedale sheep. Interleukin-6, TNF-β, IFN-γ and In this study, a immunoglobulins (Ig) G, A and E had progressive increase during the study reflected in modulation and improves the immune response in sheep. In Chapter 2, demonstrated immune response of sheep naturally infected with Haemonchus spp. in different feeding levels, submitted in native pasture and cultivated pasture. The animals were allocated into native pasture for 42 days and then transferred to cultivated pasture until 84 days. Sheep feed with pasture cultivated of oats (Avena sativa) and ryegrass (Lolium multiflorum) had reduction in OPG and increased efficiency immune response. The improvement in nutritional support to animals induced a significant increase in the immune response of sheep, characterized by peripheral eosinophilia and increased serum concentrations of IgE, IgA, IgG, TNF-β, IFN-γ and IL-6.No contexto produtivo da ovinocultura atual, o controle da infecção parasitária estabelece um desafio constante aos produtores, isso porque os parasitas gastrointestinais apresentam altas prevalências nos rebanhos, possuem elevada patogenicidade e frequentes resistências aos produtos antiparasitários. As infecções parasitárias em ovinos caracterizam-se por ocorrer simultaneamente por vários gêneros de nematódeos, no entanto, Haemonchus contortus é considerado o principal parasita gastrointestinal dos ovinos. Em algumas situações, métodos integrados de controle parasitário disponíveis tornam-se ineficazes diante de uma situação crítica, agravada pela resistência dos parasitas aos anti-helmínticos. Diante disso, a resposta imune animal torna-se ponto central na dinâmica e limitação das consequências relacionadas a infecção parasitária. A resposta imunológica sistêmica e local compõem um conjunto de mecanismos restritivos ou não, caracterizadas de acordo com a idade animal, condição fisiológica, manejo e estado nutricional. Contudo, preceitos dos quais o sistema imunológico atua e a maneira como ocorre ainda não está completamente elucidado, existindo alguns fatores a serem desvendados para que se possa estabelecer uma proteção imune segura e duradoura contra os parasitas gastrointestinais. No Capítulo 1, demonstra-se as características da resposta imune de ovinos naturalmente infectados com nematódeos gastrointestinais mantidos em campo nativo. O estudo envolveu um conjunto de técnicas imunológicas, parasitológicas e clínicas para caracterizar e estabelecer as vias da resposta imune de animais resilientes. Neste estudo, foi possível estabelecer uma fraca correlação negativa entre eosinófilos e OPG em ovinos Corriedale resilientes. Interleucina 6, TNF-β, IFN-γ e imunoglobulinas (Ig) G, A e E tiveram aumento progressivo durante o estudo refletido na modulação e melhora da resposta imunológica dos ovinos. No Capítulo 2, demonstra-se resposta imune de ovinos naturalmente infectados com Haemonchus spp. em diferentes níveis alimentares, submetidos a pastagem nativa e pastagem cultivada. Os animais foram alocados em pastagem nativa por 42 dias e em seguida transferidos para pastagem cultivada até o dia 84. Ovinos alimentados com pastagem cultivada de aveia (Avena sativa) e azevém (Lolium multiflorum) tiveram redução de OPG e aumento da eficiência da resposta imune. A melhora no suporte nutricional aos animais induziu um aumento significativo na resposta imune dos ovinos, caracterizada por eosinofilia periférica e o aumento das concentrações séricas de IgE, IgA, IgG, TNF-β, IFN-γ e IL-6.
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Muhid, Priyanesh. "The Role of Nutrients with Respect to Promoting Phytoplankton Growth and Species Composition in a Subtropical Reservoir". Thesis, Griffith University, 2011. http://hdl.handle.net/10072/366142.
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There is little known about the role of nutrients in affecting phytoplankton
growth and composition in subtropical reservoirs, unlike their temperate or
tropical counterparts. Subtropical reservoirs that are typically larger than
their temperate counterparts, with long residence times and undergo
intense stratification in summer. These characteristics favour growth of one
phytoplankton group, cyanobacteria, which tend to be the dominant taxa in
subtropical reservoirs.
This study examined the role of nutrients in promoting phytoplankton
growth and species composition in a subtropical reservoir during a period of
low inflows from the catchment. Chlorophyll a concentrations in Wivenhoe
reservoir were in the range of eutrophic to hypertrophic levels with the
highest concentrations (>60 μg L-1) recorded at the upstream sites compared
to the dam wall downstream (~10 μg L-1). Nutrient concentrations were also
higher upstream and the phytoplankton biomass was positively correlated
with depth of the water column and total dissolved phosphorus
concentrations. Mixing of the water column ensured that nutrients from the
bottom of the water column are circulated to the euphotic zone in the
shallow upstream sites, whereas at the downstream sites the water column
was deeper and more often stratified. Therefore availability of nutrients was
likely to be a key factor in increasing phytoplankton biomass.
The reservoir phytoplankton community composition also correlated with
the depth of the site and was dominated by cyanobacteria, by cell
concentrations, and by diatoms, in terms of cell biovolume. Some of the
dominant cyanobacterial groups, i.e. Aphanocapsa spp., Aphanothece spp.,
Cyanodictyon spp., Cyanocatena spp., Cyanonephron spp., Cyanogranis spp.,
Planktolyngbya spp. and Pseudanabaena spp. in this study are poorly
understood with little or no published literature on their ecology. The toxin
producing species, Cylindrospermopsis raciborskii, was also present in
relatively high concentrations near the dam wall.Thesis (PhD Doctorate)
Doctor of Philosophy (PhD)
Griffith School of Environment
Science, Environment, Engineering and Technology
Full Text
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Pereira, Fernanda Dias [UNESP]. "Análise filogenética entre Citrus spp. e Guignardia spp". Universidade Estadual Paulista (UNESP), 2012. http://hdl.handle.net/11449/92710.
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A citricultura brasileira representa um importante segmento econômico na pauta de produtos agrícolas, não só por seu expressivo valor de produção, como por sua importância na geração de empregos diretos e indiretos. No mundo, o Brasil destaca-se como maior produtor de citros, e exportador de suco concentrado de laranja. Entretanto, a citricultura ressente-se de problemas complexos, de natureza diversa, com particular destaque para o de ordem fitossanitária. Dentre esses problemas destaca-se a Mancha Preta dos Citros (MPC), causada pelo fungo Guignardia citricarpa. A doença deprecia os frutos para o mercado in natura e restringe a possibilidade de exportação. Além disso, provoca a queda prematura dos frutos e eleva o custo de produção devido à necessidade de controle. O presente trabalho teve o objetivo de estabelecer relações filogenéticas entre Citrus spp. e Guignardia spp., entender a origem evolutiva do patossistema Citrus – G. citricarpa, bem como avaliar a ocorrência de G. citricarpa como patógeno em momentos distintos na história evolutiva de Citrus. Os dados filogenéticos foram gerados utilizando-se marcadores moleculares do tipo AFLP e sequenciamento da região ITS1-5.8S-ITS2, em ambos os gêneros. As análises filogenéticas foram realizadas no programa PAUP* v.4.0b10. Os resultados das análises filogenéticas utilizando AFLP foram mais informativos que aqueles gerados com base no sequenciamento da região ITS1-5.8S-ITS2, tanto para Citrus quanto para Guignardia. As análises de AFLP permitem concluir que G. citricarpa e G. mangiferae, isoladas de C. medica, apresentam maior distância filogenética em relação aos isolados das outras espécies cítricas. A evolução do patossistema Citrus/Guignardia não pôde ser estabelecida, de forma geral, por meio da associação das filogenias...
The Brazilian citriculture represents an important economic sector in the agenda of agricultural products, not only for its impressive production value, for its importance in generating direct and indirect jobs. In the world, Brazil stands out as the largest citrus producer and exporter of concentrated orange juice. However, the citriculture suffers from complex problems of diverse nature, with particular emphasis on plant health. Among these problems highlight the Black Spot of Citrus (BSC), caused by the fungus Guignardia citricarpa. The disease depreciates the fruit for the fresh market and restricts the ability to export. Furthermore, causes the fall premature fruit and raises the cost of production due to need for control. This study aimed to establish phylogenetic relationships between Citrus and Guignardia, understanding the evolutionary origin of pathosystem Citrus - G. citricarpa, and to evaluate the occurrence of G. citricarpa pathogen at different period in evolutionary history of Citrus. The phylogenetic data were generate for both genera using AFLP molecular markers and ITS1-5.8S-ITS2 sequencing . Phylogenetic analysis were performed in the PAUP * v.4.0b10 program. The phylogenetic analysis using AFLP were more informative than ITS1-5.8S-ITS2 sequencing in both genera Citrus and Guignardia. The AFLP analysis conclude that G. citricarpa and G. mangiferae isolated from C. medica presents a larger phylogenetic distance if compared to the other citrus spp. strains. The evolution of the pathosystem Citrus/Guignardia could not be established, in general, through the association of phylogenies generated for Citrus spp. and Guignardia spp. except in the particular case of bo isolated from C. medica, which follow a pattern of associations in pathogen/endophyte
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Pereira, Fernanda Dias. "Análise filogenética entre Citrus spp. e Guignardia spp. /". Jaboticabal : [s.n.], 2012. http://hdl.handle.net/11449/92710.
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Orientador: Silvana GiuliattiBanca: Ana Lilia Alzate Marin
Banca: Gabriella Souza Cintra
Resumo: A citricultura brasileira representa um importante segmento econômico na pauta de produtos agrícolas, não só por seu expressivo valor de produção, como por sua importância na geração de empregos diretos e indiretos. No mundo, o Brasil destaca-se como maior produtor de citros, e exportador de suco concentrado de laranja. Entretanto, a citricultura ressente-se de problemas complexos, de natureza diversa, com particular destaque para o de ordem fitossanitária. Dentre esses problemas destaca-se a Mancha Preta dos Citros (MPC), causada pelo fungo Guignardia citricarpa. A doença deprecia os frutos para o mercado in natura e restringe a possibilidade de exportação. Além disso, provoca a queda prematura dos frutos e eleva o custo de produção devido à necessidade de controle. O presente trabalho teve o objetivo de estabelecer relações filogenéticas entre Citrus spp. e Guignardia spp., entender a origem evolutiva do patossistema Citrus - G. citricarpa, bem como avaliar a ocorrência de G. citricarpa como patógeno em momentos distintos na história evolutiva de Citrus. Os dados filogenéticos foram gerados utilizando-se marcadores moleculares do tipo AFLP e sequenciamento da região ITS1-5.8S-ITS2, em ambos os gêneros. As análises filogenéticas foram realizadas no programa PAUP* v.4.0b10. Os resultados das análises filogenéticas utilizando AFLP foram mais informativos que aqueles gerados com base no sequenciamento da região ITS1-5.8S-ITS2, tanto para Citrus quanto para Guignardia. As análises de AFLP permitem concluir que G. citricarpa e G. mangiferae, isoladas de C. medica, apresentam maior distância filogenética em relação aos isolados das outras espécies cítricas. A evolução do patossistema Citrus/Guignardia não pôde ser estabelecida, de forma geral, por meio da associação das filogenias... (Resumo completo, clicar acesso eletrônico abaixo)
Abstract: The Brazilian citriculture represents an important economic sector in the agenda of agricultural products, not only for its impressive production value, for its importance in generating direct and indirect jobs. In the world, Brazil stands out as the largest citrus producer and exporter of concentrated orange juice. However, the citriculture suffers from complex problems of diverse nature, with particular emphasis on plant health. Among these problems highlight the Black Spot of Citrus (BSC), caused by the fungus Guignardia citricarpa. The disease depreciates the fruit for the fresh market and restricts the ability to export. Furthermore, causes the fall premature fruit and raises the cost of production due to need for control. This study aimed to establish phylogenetic relationships between Citrus and Guignardia, understanding the evolutionary origin of pathosystem Citrus - G. citricarpa, and to evaluate the occurrence of G. citricarpa pathogen at different period in evolutionary history of Citrus. The phylogenetic data were generate for both genera using AFLP molecular markers and ITS1-5.8S-ITS2 sequencing . Phylogenetic analysis were performed in the PAUP * v.4.0b10 program. The phylogenetic analysis using AFLP were more informative than ITS1-5.8S-ITS2 sequencing in both genera Citrus and Guignardia. The AFLP analysis conclude that G. citricarpa and G. mangiferae isolated from C. medica presents a larger phylogenetic distance if compared to the other citrus spp. strains. The evolution of the pathosystem Citrus/Guignardia could not be established, in general, through the association of phylogenies generated for Citrus spp. and Guignardia spp. except in the particular case of bo isolated from C. medica, which follow a pattern of associations in pathogen/endophyte
Mestre
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Hoffmann, Christoph [Verfasser], Martin [Akademischer Betreuer] Klingenspor i Bernhard [Akademischer Betreuer] Küster. "A novel SP1/SP3 dependent intronic enhancer governing transcription of the UCP3 gene / Christoph Hoffmann. Gutachter: Bernhard Küster ; Martin Klingenspor. Betreuer: Martin Klingenspor". München : Universitätsbibliothek der TU München, 2014. http://d-nb.info/1064383084/34.
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García, Márquez Francisco, i Salas Daniel Monsalve. "VITALSUELO SPA". Tesis, Universidad de Chile, 2014. http://www.repositorio.uchile.cl/handle/2250/130045.
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Tesis para optar al grado de Magíster en AdministraciónAutores no autorizan el acceso a texto completo de su documento
García Márquez, Francisco [Parte I Análisis estratégico y de mercado],Monsalve Salas, Daniel [Parte II Análisis organizativo - financiero]
La alimentación mundial es desafío y responsabilidad de todos, siendo vital para la salud y nutrición de las personas, ya que contribuye a la vida activa y sana. La agricultura en Chile, es una actividad económica de importancia, tanto por su participación en el PIB como por el rol que cumple a nivel social, representando más del 10% del empleo nacional. Según la FAO sólo cerca del 11% de la superficie agrícola nacional se utiliza para fines productivos de cultivos anuales y permanentes, esto se debe a diversos motivos como; la urbanización, desertificación y degradación de suelos, entre otros factores. Esto hace necesario buscar un aumento en la producción nacional a través de la recuperación de suelos degradados y el alza de los rendimientos de los cultivos. Por otra parte, existe una tendencia global a producir alimentos de forma sustentable, cuidando la inocuidad alimentaria y que sea amigable con el medio ambiente. A través de Daniel Monsalve, uno de los autores de este plan de negocio, se tiene acceso a la licencia comercial de una línea de productos biológicos llamada SoilTap Inc.1, los que son complementos de la fertilización convencional. SoilTap Inc. es una línea de enmiendas biológicas, cuyo objetivo es volver el estado del suelo a niveles “pre-humano”. Esto significa enmendar todo el daño al equilibrio natural que tenía el suelo antes de ser explotado por el hombre (aplicación de fertilizantes, pesticidas, desertificación, etc.). Antiguamente, el suelo estaba compuesto por una amplia gama de microorganismos que convivían de forma simbiótica, vale decir, mutuo beneficio con las plantas. Los productos SoilTap Inc. son una combinación altamente concentrada de microorganismos simbióticos que buscan revertir la degradación del suelo producida por el hombre y devolverles su “fertilidad natural”. Los microorganismos contenidos en los productos SoilTap Inc. han sido extraídos de las tierras más fértiles del mundo, lo que permite trasladar parte de los beneficios de esos suelos a los lugares donde se apliquen los productos ya mencionados. Los beneficios otorgados permiten un mejor desarrollo y nutrición de los cultivos, generando mayores rendimientos y con alta calidad. Mediante pruebas de campo conducidas por agricultores en USA, Panamá, Guatemala, México, España y China, y ensayos formales realizados por Huazhong Agricultural University de China, se ha observado mejoras en la calidad de la cosecha y aumentos de producción entre un 20% y 200%. Es muy importante destacar que los productos SoilTap Inc., NO SON FERTILIZANTES y no compiten con ellos. Los productos SoilTap Inc. son complementarios a los fertilizantes. Se plantea crear una empresa llamada “Vitalsuelo SpA”, que represente a la marca SoilTap Inc. en Chile, estableciendo canales de distribución, soporte técnico y fuerza de venta propia que asista a los clientes en terreno. Una vez implementado con éxito los productos SoilTap Inc., se ampliará la oferta de productos, incorporando otros adicionales de características similares y complementarias, Ej.: fertilizantes ecológicos y convencionales, productos que ayudan a la conservación y mayor aprovechamiento del agua, entre otros. En una primera etapa y antes de invertir grandes sumas de dinero en la puesta en marcha de Vitalsuelo SpA., se sugiere realizar ensayos formales a través del Instituto Nacional de Investigaciones Agropecuarias (INIA), para que una institución con respaldo nacional, valide en condiciones locales los resultados obtenidos en otras partes del mundo. Una vez obtenidos los resultados que demuestran un incremento en producción y/o de la calidad de los productos, aumentando el beneficio neto del productor en más de $1.000.000 de pesos por hectárea, se pasará a la fase II, “el desarrollo de la empresa”. Para efectos de este plan de negocio, se asume que los resultados de las pruebas nacionales, permitirán el desarrollo del resto del plan. Se busca posicionar estos productos como Premium, destacándose por su desempeño y beneficio entregado al agricultor. Para llevar a cabo este plan de negocio, se requiere un aporte de capital aproximado a USD 909.000, el cual debe ser proporcionado durante los primeros 4 años del proyecto según los requerimientos anuales para investigación y capital de trabajo. El retorno esperado es de 35,19% y el valor presente neto de Vitalsuelo SpA es de USD 1,8605 millones, utilizando una tasa de descuento de 17,42%. Vitalsuelo SpA, es una empresa que busca ofrecer un aporte tangible a productores agrícolas, elevando los rendimientos de sus cultivos y mejorando la calidad de sus cosechas, esto además, en línea con las actuales tendencias y desafíos propuestos por la sociedad. El riesgo asociado a la ejecución y éxito del proyecto, se hace atractivo producto de la tasa interna de retorno sobre el capital invertido.
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Hansen, Sjölander Stéphanie. "Skievvá Spa". Thesis, KTH, Arkitektur, 2013. http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-122980.
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Skievvá spa, an opportunity to discover and take part of the peculiar landscape in one of the most beautiful fjords in Northern Norway, Skjomenfjorden (Narvik).An opportunity to discover the “real” Narvik. An architecture that reinforces the impressions and the sensations in the landscape on different scales. Here the bath is the central space. A spa, a landscape hotel and a trekking station.The complex scale of nature is intensified through the large scale framing architecture. The unique and custom site with its program strengthens the tourism industry and attract more visitors to the North Norwegian mountains.ountains and Narvik.I have always been fascinated by the tremendous and dramatic scenographic landscape of northern Norway. This is where I have my roots. I visit Narvik and Skjomen(Skievvá) every year since have family here, and also I’m addicted of the sensational nature and the purifying boost and revitalisation it gives you. I want more people to visit and explore this treasure. The scenery and outlooks in different scales have been an important part of my process. The buildings have framings and windows to frame and enhence the beautiful scenery and elements in the environment. In an open complex landscape like this, the relationship between volumes is very important, spacing and proportions. An architecture that blends in with the surrounding and competes with it at the same time. I wanted to give the landscape, the mountain, a scale.The project is largely about architectural elements meeting the scenery and terrain and how to encounter the landscape. But just as much about how the architecture affects and enables one to take advantage of this incredible landscape.Skievvá spa, en möjlighet att upptäcka och ta del av den säregna landskapet i en av de vackraste fjordar i Nordnorge, Skjomenfjorden (Narvik). En möjlighet att upptäcka den "riktiga" Narvik. En arkitektur som förstärker intrycken och förnimmelser i landskapet på olika skalor. Här badet är det centrala utrymmet. Ett spa, ett landskap hotell och en vandringsstation. Komplexiteten i naturens skala intensifieras genom storskalig inramande arkitektur. Den unika och anpassade platsen med sitt program stärker turistnäringen och lockra fler besökare till de nordliga norska fjällens och Narvik. Jag har alltid varit fascinerad av den enorma och dramatiska scenografiska landskap i norra Norge. Det är där jag har mina rötter. Jag besöker Narvik och Skjomen (Skievvá) varje år sedan har familj här, och även jag beroende av den sensationella karaktär och renande boost och vitalisering det ger dig. Jag vill att fler människor att besöka och utforska denna skatt. Landskap och synsätt i olika skalor har varit en viktig del av min process. Byggnaderna har framings och fönster till ramen och enhence den vackra naturen och elementen i miljön. I ett öppet komplext landskap som denna, är förhållandet mellan volymerna är mycket viktigt, avstånd och proportioner. En arkitektur som smälter in i omgivningen och konkurrerar med den på samma gång. Jag ville ge landskapet, berg, en skala.Projektet är till stor del om arkitektoniska element som uppfyller landskap och terräng och hur man möter landskapet. Men lika mycket om hur arkitekturen påverkar och möjliggör att man kan dra nytta av denna fantastiska landskapet.
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Trkal, Ondřej. "Rozpoznávání SPZ". Master's thesis, Vysoké učení technické v Brně. Fakulta elektrotechniky a komunikačních technologií, 2016. http://www.nusl.cz/ntk/nusl-240811.
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The thesis deals with analysis and design of system for automatic localization and recognition of the license plate. The input images are from different sources, and contain large scenic and weather variations. The aim was to create a system able to find the licence plate on the image and recognize its alphanumeric figure. In this work, there is a focus on analysis and implementation of localization and optical character recognition methods. One own and four other localization methods are compared. There are also compared three classifiers for optical character recognition. Localization and OCR methods are tested on real data and evaluated in accordance with the calculated evaluation parameters. The work also contains sensitivity analysis of the proposed system.
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Barbara, Jean Carlos Alves. "Avaliação do perfil sanitário de urubu-de-cabeça-preta (Coragyps atratus) em ambiente urbano". Universidade de São Paulo, 2015. http://www.teses.usp.br/teses/disponiveis/10/10133/tde-18082015-131331/.
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O urubu-de-cabeça-preta (Coragyps atratus) é uma ave de vida livre com ampla distribuição no Brasil. Esta espécie é comumente encontrada em áreas urbanas concentrando-se em locais de deposição de lixo. O fato de se alimentarem de carcaças em decomposição facilita o contato de urubus-de-cabeça-preta com muitos patógenos. No entanto, ainda não está clara qual a real implicação de muitos desses microrganismos para a saúde dos mesmos. Assim, o objetivo desse estudo foi investigar a ocorrência de alguns patógenos selecionados, avaliar o perfil hematológico e a microbiota cloacal de C. atratus em ambiente urbano. Para isso, amostras de sangue, soro e swab cloacal foram obtidos de 120 urubus de vida-livre capturados na Fundação Parque Zoológico de São Paulo, SP. A prova de soroaglutinação rápida (SAR) foi utilizada na detecção de anticorpos contra Salmonella Pullorum/Gallinarum, Mycoplasma synoviae e M. gallisepticum. O teste de aglutinação em látex (AL) foi utilizado para a pesquisa de antígeno de Cryptococcus neoformans. Foram utilizadas técnicas convencionais de hematologia, microbiologia e testes de sensibilidade microbiana. Das amostras de soro analisadas pela SAR, 15% foram reagentes para M. gallisepticum. Anticorpos contra S. Pullorum/Gallinarum e M. synoviae não foram detectados. Nenhuma amostra foi positiva para C.neoformans ou para hemoparasitas. A média e o desvio padrão dos seguintes valores hematológicos foram obtidos para 61 aves: eritrócitos (1.8x10¹²/L); leucócitos (13,11x10/L); hemoglobina (10,4 g/dL); hematócrito (48,44%); VCM (275,1 fL); HCM (42 pg); CHCM (15,8 g/dL); proteína sérica total (3,76 g/dL); heterófilos (78%); linfócitos (13,5%); eosinófilos (5,4%); monócitos (2,8%); basófilos (0,1%); trombócitos (14,14x10/L). De 75 colônias bacterianas isoladas de 20 swabs cloacais, 78,7% foram Gram-positivas e 21,3% Gram-negativas, sendo Enterococcus sp. o gênero mais frequente. Aproximadamente 86,7% das cepas isoladas foram resistentes a pelo menos um dos antibióticos testados. Cepas de Bacillus sp. e Enterococcus casseliflavus apresentaram resistência a sete dos oito antibióticos testados. Leveduras não foram isoladas em nenhumas das culturas. As informações obtidas nessa pesquisa são de suma importância, uma vez que poucos estudos avaliam o estado de saúde de urubus no mundo.Black vulture (Coragyps atratus) is a free-living bird widely distributed across Brazil. These birds feed on rotting carcasses and large groups are commonly found in urban areas, including rubbish dumps. By feeding on decomposing carcasses, they are often exposed to innumerous pathogens. However, the role of infectious microorganisms on vultures health still need to be clarify. Thus, the aim of this study was to investigate the occurrence of selected infectious agents, the hematological profile and cloacal microbiota of black vulture in urban areas. Therefore, blood, serum and cloacal swabs were obtained from 120 free-living vultures trapped in Fundação Parque Zoológico de São Paulo, SP. The rapid seroagglutination test (RST) was performed for detection of antibodies against Salmonella Pullorum/Gallinarum, M. synoviae and M. gallisepticum. Furthermore, latex agglutination test was used to detect Cryptococcus neoformans \' antigen. Conventional techniques for hematology, microbiology and antimicrobial susceptibility testing were performed. From the serum samples analyzed by RST, 15% were positive for M. gallisepticum, antibodies against S. Pullorum/Gallinarum and M. synoviae were not detected. None sample was positive to Cryptococcus neoformans or hemoparasites. Mean and standard deviation from the following hematological values were obtained for 61 birds: erythrocytes (1.8x10¹²/L); leukocytes (13.11x10/L); hemoglobin (10.4 g/dL); hematocrit (48.44%); MCV (275,1 fL); MCH (42 pg); MCHC (15,8 g/dL); total serum protein (3.76 g/dL); heterophils (78%); lymphocytes (13.5%); eosinophils (5.4%); monocytes (2.8%); basophils (0,1%); thrombocyte (14.14x10/L). From 75 bacterial colonies isolated from 20 cloacal swabs, 78.7% were Gram-positive and 21.3% were Gram-negative. Enterococcus sp. was the most frequent genus. Approximately 86.7% of the isolated strains were resistant to at least one of the antibiotic tested. Bacillus sp. and Enterococcus casseliflavus strains shown resistance to seven in eight antibiotics tested. Yeasts were not isolated. The information obtained in this research is of paramount important since few studies have been carried out on the vultures health condition in the world.
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Vargas, Sarah Regina. "Produção de hidrogênio por Chlamydomonas spp. e Anabaena spp". Universidade de São Paulo, 2016. http://www.teses.usp.br/teses/disponiveis/18/18138/tde-21032017-100636/.
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O uso intensificado de combustíveis fósseis como fonte de energia, vê-se a necessidade do desenvolvimento de novas tecnologias, principalmente as renováveis, como o hidrogênio, que possui vantagens por ser elemento abundante no universo, ser renovável e não poluente. A utilização de microalgas e cianobactérias é uma alternativa para a produção de biohidrogênio a partir da quebra da água e de compostos orgânicos. De acordo com isso, nesta pesquisa foram testados diversos fatores físico-químicos e nutricionais nas condições de cultivo de cepas de Chlamydomonas spp. e Anabaena spp. Para tanto, cepas selecionadas foram cultivadas em duas fases experimentais, a primeira aeróbia e a segunda anaeróbia, para proporcionar produção de hidrogênio por biofotólise direta anaeróbia, via hidrogenase, sob privação de enxofre para a clorofícea, e de nitrogênio para a cianobactéria, estimulando para esta também a produção por biofotólise indireta, via nitrogenase. A cepa com melhor produtividade de hidrogênio, de cada gênero, foi selecionada para a etapa de otimização das fases experimentais de cultivo. Durante os ensaios foram realizadas análises de produção máxima, velocidade de produção, volume e produtividade de hidrogênio, além de análises de concentração de biomassa, físico-químicas, bioquímicas e geração de subprodutos. O método utilizado foi eficiente para produção de hidrogênio e ficou comprovada a diferença de produção de hidrogênio entre diferentes cepas. Anabaena sp. obteve produtividade média de hidrogênio quatro vezes maior, aproximadamente de 76,8 µmol.L-1.h-1, comparada a C. reinhardtii, com média de 18,6 µmol.L-1.h-1.The intensifying use of fossil fuels as energy source, one sees the need to develop new technologies, especially renewable, such as hydrogen. This has advantages because hydrogen is an abundant element in the universe, be renewable and non-polluting. The use of microalgae and cyanobacteria is an alternative for the production of bio-hydrogen of breaking water and organic compounds. Accordingly, in this study were tested several physic-chemical factors and nutrition in growing conditions of Chlamydomonas spp. and Anabaena spp. strains. For this purpose, strains selected were cultured in two experimental phases, first aerobic and second anaerobic, to hydrogen production by direct biofotolise anaerobic, via hydrogenase, under sulfur deprived to chlorofycea, and nitrogen to cyanobacterium, for this also to production by indirect biofotolise, via nitrogenase. The strain with highest productivity of hydrogen, of each gender, was selected for the optimization of the experimental stages of cultivation. During the tests were analyzes of maximum production, velocity, volume and productivity of hydrogen, and analysis of biomass concentration, physic-chemical, biochemical and generation of by-products. The method used was efficient for the production of hydrogen and was different between strains. Anabaena sp. obtained average yield four times highest, approximately 76.8 µmol. L-1.h-1compared to C. reinhardtii, averaging 18.6 µmol. L-1.h-1.
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Moffet, Matthew Durwin. "Identifying regions of conserved synteny between pea (Pisum spp.), lentil (Lens spp.), and bean (Phaseolus spp.)". Thesis, Montana State University, 2006. http://etd.lib.montana.edu/etd/2006/moffet/MoffetM1206.pdf.
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The identification of conserved synteny in legumes can facilitate many different types of gene discovery. Techniques like marker assisted selection and the candidate gene approach can benefit greatly by identifying conserved synteny and genes located within those regions. Both Pisum and Phaseolus have detailed linkage maps, but a limited number of markers have been located in both species. In the present study I mapped 21 genes in Phaseolus vulgaris, 16 of which had already been located on the Lens and Pisum sativum linkage maps, the markers were used to look for conserved synteny between Pisum, Lens and Phaseolus. In particular, I was able to target marker/gene-rich regions of pea linkage groups V and VII, as well as pea linkage group III, with Pisum STS markers and universally designed gene-specific markers already located on the pea linkage map. About 50% of the tested genes amplified an appropriate sized fragment in Phaseolus, but less than 40 % of the gene-specific markers showed polymorphism by cleaved amplified polymorphic sequence (CAPS) analysis in bean. The data reveals little evidence for extensive gene order conservation, and even some closely linked (<5cM) loci in Pisum are not linked in Phaseolus vulgaris. The only example of conserved synteny was approximately 15cM on pea and lentil linkage group V and bean linkage group 1. Paal, Enolase, and TufM were first identified in the syntenic area and allowed identification of fin/det, one of several TFL genes already mapped in bean, as another orthologous loci between pea and bean. Finding conservation of synteny with Paal, identified Paal3 and TFL1 genes as linked loci in Arabidopsis thaliana on linkage group 5. The pea locus Paal1,2 is then speculated to be a tandem duplication of a Paal3 homolog in a ancient common ancestor and probably occurring after the speciation of Pisum.
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Berseneff, Boris. "Réglage de la tension dans les réseaux de distribution du futur". Phd thesis, Université de Grenoble, 2010. http://tel.archives-ouvertes.fr/tel-00575940.
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Les réseaux de distribution sont aujourd'hui confrontés à un accroissement significatif de la Génération d'Énergie Dispersée. Cet accroissement pose, notamment, des problèmes des tension que les moyens de réglage de la tension utilisés aujourd'hui ne parviennent plus à éviter. En conséquence, la capacité d'accueil des réseaux de distribution est aujourd'hui très limitée. Cette thèse propose une méthode de réglage de tension novatrice appelée OMD pour Optimisation Mixte Découplée. Cette méthode se base sur une gestion optimale en temps réel de la puissance réactive des GED et des variables discrètes du système. Les simulations entreprises montrent que l'algorithme développé permet d'augmenter significativement le nombre de GED connectées au réseau et d'optimiser les pertes Joule. De plus, l'OMD apporte une plus value intéressante de par sa modularité et sa flexibilité en comparaison avec d'autres méthodes proposées dans la littérature. Une étude complémentaire montre que le concept de l'OMD peut être repris et appliqué à d'autres systèmes. Ainsi, un réglage de tension optimal, basé sur l'OMD a été développé pour les parcs éoliens. Il permet un contrôle optimal de la tension à l'intérieur des parcs tout en assurant le maintien d'une consigne de puissance réactive ou de tension au point de connexion avec le réseau. Le réglage de tension proposé dans cette thèse est donc une des solutions qui pourra permettre un meilleur fonctionnement des réseaux de distribution du futur : les Smart Grids
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Åklint, Peter. "Övergripande kvalitets och kapabilitets granskning av produktionsprocessen – en implementering av SPS. : Overall Quality and Capability Study of the Production Process – an Implement of SPC". Thesis, Linköping University, Department of Management and Engineering, 2007. http://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-10432.
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Quality was earlier only an issue for the company’s quality departments but today is the entire organization involved in quality- and improvements work. With the customers in focus, both external and internal, quality and improvement work should be integrated in the daily activities. The management shall state goals and visions together with a policy. Through participation the correct requirements for a successful work are created.
With SPC, statistics process control, there is an opportunity to perceive variations in the process and with the correct tools make it stable. Examples of tools that can be used are the seven QC – tools and the PDCA – cycles, which are presented more detailed in the report.
By using SPC and the information given by it, a measure of the process capability within fixed tolerances on the produced parts can be achieved.
The analysis in this report has been made in order to verify the capability and variations in the measuring system used within a selected production sector.
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Wijmeersch, Gaëlle. "Etude de la régulation transcriptionnelle du virus de la leucémie bovine: rôle des facteurs de transcription Sp1/Sp3 et de la méthylation de l'ADN". Doctoral thesis, Universite Libre de Bruxelles, 2008. http://hdl.handle.net/2013/ULB-DIPOT:oai:dipot.ulb.ac.be:2013/210055.
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Étude de la régulation transcriptionnelle du virus de la leucémie bovine :rôle des facteurs de transcription Sp1/Sp3 et de la méthylation de l’ADNDoctorat en sciences, Spécialisation biologie moléculaire
info:eu-repo/semantics/nonPublished
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Mao, Lujia [Verfasser], Todd B. [Gutachter] Marder i Kálmán J. [Gutachter] Szabó. "Transition Metal-Catalyzed Construction of Benzyl/Allyl sp3 and Vinyl/Allenyl sp2 C-B Bonds / Lujia Mao ; Gutachter: Todd B. Marder, Kálmán J. Szabó". Würzburg : Universität Würzburg, 2018. http://d-nb.info/116957288X/34.
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Souza, Marinaldo Fernando de. "Das formas instituídas às contradições reveladas : análise da práxis educacional das políticas públicas de assistência social /". Araraquara : [s.n.], 2010. http://hdl.handle.net/11449/88560.
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Orientador: Dulce Consuelo Andreatta WhitakerBanca: Rosemeire Aparecida Scopinho
Banca: Ângela Viana Machado Fernandes
Resumo: Com a implantação do Sistema Único de Assistência Social (SUAS), as práticas socioeducativas vêm se destacando como modelo de atuação no âmbito da Política Nacional de Assistência Social, cujo objetivo é o processo de conscientização e superação da condição de pobreza de pessoas, famílias e comunidades beneficiadas pelos serviços. Pretende-se com isso, romper com as velhas práticas assistencialistas, que visam tão somente a provisão de recursos materiais através de ações de caridade e, valorizar a educação como prática da liberdade. Sendo assim, este trabalho teve como objetivo central, compreender o modelo de educação social praticado em instituições incumbidas de oferecer serviços de assistência social - tanto de forma direta através das unidades de Centros de Referência de Assistência Social (CRAS), como indiretamente nas entidades e organizações de Assistência Social que dão apoio aos serviços. A premissa inicial que fora submetida ao objetivo central, é a de que as ações educativas desenvolvidas nesses espaços são obstaculizadas por olhares preconceituosos sobre as pessoas atendidas. Trabalhou-se com a hipótese de que as micro-relações estabelecidas no trabalho socioassistencial são instrumentos de circulação e manutenção de poder e dominação. Para a coleta de dados, foram utilizados procedimentos metodológicos de inspiração etnográfica - descrições em Diário Campo, técnicas de grupo e observações participantes - que permitiram captar e analisar conhecimentos acumulados, hábitos, valores e estratégias de atuação dos trabalhadores ligados aos serviços. A partir da análise de conteúdo das falas e da observação dos métodos de trabalho de profissionais diretamente ligados às ações socioeducativas, percebeu-se que os vínculos estabelecidos entre trabalhadores e usuários dos serviços, acabam fortalecendo... (Resumo completo, clicar acesso eletrônico abaixo)
Resumen: Con la implantación del Sistema Único de Asistencia Social (SUAS), las prácticas socioeducativas se vienen destacando como modelo de acción en el ámbito de la Política Nacional de Asistencia Social, cuyo objetivo es el proceso de concienciación y superación de la condición de pobreza de personas, familias y comunidades beneficiadas por los servicios sociales. Se pretende, por una parte romper con las viejas prácticas asistenciales, que se preocupan solamente de la provisión de recursos materiales a través de acciones de caridad y, por otra, valorizar la educación como práctica de la libertad. Coherente a lo anteriormente expuesto, esta investigación tuvo como objetivo central, comprender el modelo de educación social practicado en instituciones que ofrecen servicios de asistencia social - tanto de forma directa a través de las unidades de Centros de Referencia de Asistencia Social (CRAS), como indirectamente en las entidades y organizaciones de Asistencia Social que dan apoyo a los servicios. La premisa inicial afiliada al objetivo central, es la de que las acciones educativas desarrolladas en esos espacios son obstaculizadas por prejuicios sobre las personas atendidas. Se trabajó con la hipótesis de que las micro-relaciones establecidas en el trabajo socioasistencial son instrumentos de circulación y manutención de poder y dominación. Para la recolección de datos se utilizaron procedimientos metodológicos de corte etnográfico - descriptivo en Diario Campo, técnicas de grupo y observaciones participantes - que permitieron captar y analizar conocimientos acumulados, hábitos, valores y estrategias de actuación de los trabajadores que prestan estos servicios. A partir del análisis de contenido de los discursos y de la observación de los métodos de trabajo de profesionales directamente relacionados a las acciones socioeducativas, se percibió que los vínculos... (Resumen completo clicar acceso eletronico abajo)
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