Gotowe bibliografie tematyczne / SmpB

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Gotowa bibliografia na temat „SmpB”

Autor: Grafiati

Data publikacji: 4 czerwca 2021

Data aktualizacji: 10 lutego 2022

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Artykuły w czasopismach na temat "SmpB"

Okan, Nihal A., Patricio Mena, Jorge L. Benach, James B. Bliska i A. Wali Karzai. "The smpB-ssrA Mutant of Yersinia pestis Functions as a Live Attenuated Vaccine To Protect Mice against Pulmonary Plague Infection". Infection and Immunity 78, nr 3 (11.01.2010): 1284–93. http://dx.doi.org/10.1128/iai.00976-09.

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ABSTRACT The bacterial SmpB-SsrA system is a highly conserved translational quality control mechanism that helps maintain the translational machinery at full capacity. Here we present evidence to demonstrate that the smpB-ssrA genes are required for pathogenesis of Yersinia pestis, the causative agent of plague. We found that disruption of the smpB-ssrA genes leads to reduction in secretion of the type III secretion-related proteins YopB, YopD, and LcrV, which are essential for virulence. Consistent with these observations, the smpB-ssrA mutant of Y. pestis was severely attenuated in a mouse model of infection via both the intranasal and intravenous routes. Most significantly, intranasal vaccination of mice with the smpB-ssrA mutant strain of Y. pestis induced a strong antibody response. The vaccinated animals were well protected against subsequent lethal intranasal challenges with virulent Y. pestis. Taken together, our results indicate that the smpB-ssrA mutant of Y. pestis possesses the desired qualities for a live attenuated cell-based vaccine against pneumonic plague.

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Rae, Christopher D., Yuliya Gordiyenko i V. Ramakrishnan. "How a circularized tmRNA moves through the ribosome". Science 363, nr 6428 (14.02.2019): 740–44. http://dx.doi.org/10.1126/science.aav9370.

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During trans-translation, transfer-messenger RNA (tmRNA) and small protein B (SmpB) together rescue ribosomes stalled on a truncated mRNA and tag the nascent polypeptide for degradation. We used cryo–electron microscopy to determine the structures of three key states of the tmRNA-SmpB-ribosome complex during trans translation at resolutions of 3.7 to 4.4 angstroms. The results show how tmRNA and SmpB act specifically on stalled ribosomes and how the circularized complex moves through the ribosome, enabling translation to switch from the old defective message to the reading frame on tmRNA.

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Une, M., D. Kurita, A. Muto i H. Himeno. "Trans-translation by tmRNA and SmpB". Nucleic Acids Symposium Series 53, nr 1 (1.09.2009): 305–6. http://dx.doi.org/10.1093/nass/nrp153.

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Sundermeier, Thomas R., i A. Wali Karzai. "Functional SmpB-Ribosome Interactions Require tmRNA". Journal of Biological Chemistry 282, nr 48 (2.10.2007): 34779–86. http://dx.doi.org/10.1074/jbc.m707256200.

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Shin, Ji-Hyun, i Chester W. Price. "The SsrA-SmpB Ribosome Rescue System Is Important for Growth of Bacillus subtilis at Low and High Temperatures". Journal of Bacteriology 189, nr 10 (16.03.2007): 3729–37. http://dx.doi.org/10.1128/jb.00062-07.

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ABSTRACT Bacillus subtilis has multiple stress response systems whose integrated action promotes growth and survival under unfavorable conditions. Here we address the function and transcriptional organization of a five-gene cluster containing ssrA, previously known to be important for growth at high temperature because of the role of its tmRNA product in rescuing stalled ribosomes. Reverse transcription-PCR experiments detected a single message for the secG-yvaK-rnr-smpB-ssrA cluster, suggesting that it constitutes an operon. However, rapid amplification of cDNA ends-PCR and lacZ fusion experiments indicated that operon transcription is complex, with at least five promoters controlling different segments of the cluster. One σA-like promoter preceded secG (P1), and internal σA-like promoters were found in both the rnr-smpB (P2) and smpB-ssrA intervals (P3 and PHS). Another internal promoter lay in the secG-yvaK intercistronic region, and this activity (PB) was dependent on the general stress factor σB. Null mutations in the four genes downstream from PB were tested for their effects on growth. Loss of yvaK (carboxylesterase E) or rnr (RNase R) caused no obvious phenotype. By contrast, smpB was required for growth at high temperature (52°C), as anticipated if its product (a small ribosomal binding protein) is essential for tmRNA (ssrA) function. Notably, smpB and ssrA were also required for growth at low temperature (16°C), a phenotype not previously associated with tmRNA activity. These results extend the known high-temperature role of ssrA and indicate that the ribosome rescue system is important at both extremes of the B. subtilis temperature range.

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Shimizu, Yoshihiro, i Takuya Ueda. "The role of SmpB protein intrans-translation". FEBS Letters 514, nr 1 (1.02.2002): 74–77. http://dx.doi.org/10.1016/s0014-5793(02)02333-5.

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Kurita, Daisuke, Akira Muto i Hyouta Himeno. "tRNA/mRNA Mimicry by tmRNA and SmpB inTrans-Translation". Journal of Nucleic Acids 2011 (2011): 1–9. http://dx.doi.org/10.4061/2011/130581.

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Since accurate translation from mRNA to protein is critical to survival, cells have developed translational quality control systems. Bacterial ribosomes stalled on truncated mRNA are rescued by a system involving tmRNA and SmpB referred to astrans-translation. Here, we review current understanding of the mechanism oftrans-translation. Based on results obtained by using directed hydroxyl radical probing, we propose a new type of molecular mimicry duringtrans-translation. Besides such chemical approaches, biochemical and cryo-EM studies have revealed the structural and functional aspects of multiple stages oftrans-translation. These intensive works provide a basis for studying the dynamics of tmRNA/SmpB in the ribosome.

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Hanawa-Suetsugu, K. "SmpB functions in various steps of trans-translation". Nucleic Acids Research 30, nr 7 (1.04.2002): 1620–29. http://dx.doi.org/10.1093/nar/30.7.1620.

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Kovács, L., Klára Megyeri, Anna Juhász, Anikó Zaja i A. Miczák. "Cloning, expression and purification of smpb fromMycobacterium tuberculosis". Acta Microbiologica et Immunologica Hungarica 51, nr 3 (listopad 2004): 297–302. http://dx.doi.org/10.1556/amicr.51.2004.3.7.

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Zhang, Yidong, Zebin Liu, Yanqiong Tang, Xiang Ma, Hongqian Tang, Hong Li i Zhu Liu. "Cbl upregulates cysH for hydrogen sulfide production in Aeromonas veronii". PeerJ 9 (9.09.2021): e12058. http://dx.doi.org/10.7717/peerj.12058.

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Endogenous hydrogen sulfide (H2S) is generated in many metabolism pathways, and has been recognized as a second messenger against antibiotics and reactive oxygen species (ROS). In Aeromonas veronii, Small Protein B (SmpB) plays an important role in resisting stress. The absence of smpB could trigger sulfate assimilation pathway to adapt the nutrient deficiency, of which was mediated by up-regulation of cbl and cys genes and followed with enhancing H2S production. To figure out the mutual regulations of cbl and cys genes, a series of experiments were performed. Compared with the wild type, cysH was down-regulated significantly in cbl deletion by qRT-PCR. The fluorescence analysis further manifested that Cbl had a positive regulatory effect on the promoter of cysJIH. Bacterial one-hybrid analysis and electrophoretic mobility shift assay (EMSA) verified that Cbl bound with the promoter of cysJIH. Collectively, the tolerance to adversity could be maintained by the production of H2S when SmpB was malfunctioned, of which the activity of cysJIH promoter was positively regulated by upstream Cbl protein. The outcomes also suggested the enormous potentials of Aeromonas veronii in environmental adaptability.

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Rozprawy doktorskie na temat "SmpB"

Holden, James Anthony, i jamesholden@netspace net au. "Vaccination Strategies for the Prevention of Swine Dysentery". RMIT University. Applied Sciences, 2006. http://adt.lib.rmit.edu.au/adt/public/adt-VIT20070112.122102.

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The SmpA outer membrane lipoprotein of B. hyodysenteriae has several characteristics that indicate the potential to protect against swine dysentery (SD). It localises to the outer membrane and antibodies directed against SmpA can prevent the growth of B. hyodysenteriae in vitro. There is some variation observed in the distribution and expression of the SmpA lipoprotein, suggesting that vaccination with SmpA may not provide protection against challenge with a heterologous B. hyodysenteriae strain. This study has characterised the variation at the smpA locus, and in the process has identified a novel gene, smpB. There is very low similarity between smpB and smpA, with the exception of an identical lipoprotein signal sequence. This suggests that SmpB may be translocated to the outer membrane of B. hyodysenteriae in a similar fashion to SmpA. The results described in this thesis indicate that strains of B. hyodysenteriae harbour either smpA or smpB, but not both, explaining the earlier results of Turner et al. (1991). The presumed outer membrane location of SmpB lead to further investigations into its potential to protect mice from infection with B. hyodysenteriae. Swine Dysentery is a inflammatory disease of the swine colon. Therefore it is believed that a mucosal immune response may provide increased protection against challenge. In this study, vaccination of mice with recombinant SmpB elicited high levels of serum antibodies, induced the production of Interleukin-4 producing T lymphocytes and decreased the observed histological effects after challenge with virulent B. hyodysenteriae. In efforts to increase the protected conferred by vaccination with SmpB, recombinant Salmonella typhimurium STM-1 vaccines were created to express SmpB or deliver DNA vaccines encoding SmpB. Vaccination with these recombinant Salmonella vectors did not induce a measurable SmpB specific immune response. Macrophage survival and plasmid stability studies indicated that this was due to instability of the expression plasmids in STM-1. Although SmpB will only ever protect against strains of B. hyodysenteriae harbouring smpB, these results indicate that with further research, SmpB (and SmpA) may contribute to protection from SD. Toxin production is an important aspect of the pathogenesis of many pathogenic bacteria. Vaccination with attenuated toxins is commonly used to prevent disease. In this study, the B. hyodysenteriae â-haemolysin HlyA was used to vaccinate mice to determine the protection induced after challenge. Vaccination of mice with recombinant HlyA induced significant levels of serum antibodies and lowered the observed pathological effects after challenge of vaccinated mice with virulent B. hyodysenteriae. In an attempt to increase the mucosal immune response and therefore the protection afforded after vaccination with HlyA, recombinant S. typhimurium STM-1 strains were created to express HlyA or deliver DNA vaccines encoding HlyA. Similar to the recombinant STM-1 vaccines expressing SmpB, a HlyA specific immune response was not observed by ELISA or ELISPOT analysis. Plasmid stability trials revealed that the inability to induce a detectable HlyA specific immune response by recombinant STM-1 vaccination may be due to ins tability of the plasmids. Outer membrane proteins are often important components of vaccines against bacterial and viral pathogens. Considering the variation observed in the smpA locus in this study resulting in the identification of smpB, further investigation into the distribution and conservation of outer membrane encoding genes in B. hyodysenteriae strains was undertaken. In particular, the blpAEFG, vspABCD and vspEFGH clusters were analysed for their distribution. It was demonstrated that genes that are B. hyodysenteriae specific (vspABCD and vspEFGH) displayed higher levels of polymorphism than those that are distributed amongst non-pathogenic species, such as B. innocens (which contains blpAEFG). This suggests that the variation in the vspABCD and vspEFGH clusters amongst B. hyodysenteriae strains may be a result of the exposure to the host immune system. Further investigation was undertaken by PFGE analysis and 2D-gel electrophoresis, to analyse genomic and proteomic variation at a global level. Although strains of B. hyodyse nteriae produced several different electrophoretic types (ET) upon PFGE analysis, only limited correlation between the PFGE ET, the polymorphisms in vspABCD and vspEFGH and the presence of smpA/smpB were observed. 2D-gel electrophoresis analysis of outer membrane preparations of two B. hyodysenteriae strain revealed several distinct differences in the outer membrane between B. hyodysenteriae strains. The observed differences in the proteins contained in the outer membrane of B. hyodysenteriae is important for vaccine design, as the induction of cross protection between strains of B. hyodysenteriae is essential for a effective vaccine.

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Miller, Mickey R. "The Role of SmpB in Licensing tmRNA Entry into Stalled Ribosomes". BYU ScholarsArchive, 2013. https://scholarsarchive.byu.edu/etd/4162.

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Ribosomes translate the genetic information contained in mRNAs into protein by linking together amino acids with the help of aminoacyl-tRNAs. In bacteria, protein synthesis stalls when the ribosome reaches the 3'-end of truncated mRNA transcripts lacking a stop codon. Trans-translation is a conserved bacterial quality control process that rescues stalled ribosomes. Transfer-messenger RNA (tmRNA) and its protein partner SmpB mimic a tRNA by entering the A site of the ribosome and accepting the growing peptide chain. The ribosome releases the truncated mRNA and resumes translation on the tmRNA template. The open reading frame found on tmRNA encodes a peptide tag that marks the defective nascent peptide for proteolysis. A stop codon at the end of the open reading frame allows the ribosome to be recycled and engage in future rounds of translation.The entry of tmRNA into stalled ribosomes presents a challenge to our understanding of ribosome function because during the canonical decoding process, the ribosome specifically recognizes the codon-anticodon duplex formed between tRNA and mRNA in the A site. Recognition of proper base-pairing leads to conformational changes that accelerate GTP hydrolysis by EF-Tu and rapid accommodation of the tRNA into the ribosome for peptidyl transfer. The puzzle is that tmRNA enters stalled ribosomes and reacts with the nascent peptide in the absence of a codon-anticodon interaction. Instead, SmpB binding in the decoding center begins the rescue process, but it has been unclear how SmpB licenses tmRNA entry into stalled ribosomes. We analyzed a series of SmpB and ribosomal RNA mutants using pre-steady-state kinetic assays for EF-Tu activation and peptidyl transfer. Although the conserved 16S nucleotides A1492 and A1493 play an essential role in canonical decoding, they play little or no role in EF-Tu activation or peptidyl transfer to tmRNA. In contrast, a third nucleotide, G530, stacks with the side chain of SmpB residue His136, inducing conformational changes that lead to GTP hydrolysis by EF-Tu. A portion of the C-terminal tail forms a helix within the mRNA channel, monitoring the length of mRNA bound in the ribosome to avoid aborting productive protein synthesis. Helix formation in the mRNA channel is essential for accommodation and peptidyl transfer, but not for GTP hydrolysis. We show that conserved residues in the tail are essential for EF-Tu activation, accommodation, or translocation to the P site. Our findings lead to a clearer model of how the tmRNA-SmpB complex enters stalled ribosomes.

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Cazier, DeAnna June. "The Role of SmpB in the Early Stages of Trans-Translation". BYU ScholarsArchive, 2009. https://scholarsarchive.byu.edu/etd/2126.

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Ribosomes stall on defective messenger RNA transcripts in eubacteria. Without a mechanism to release stalled ribosomes, these cells would die. Transfer-messenger RNA (tmRNA) and small protein B (SmpB) reactivate stalled ribosomes in a process known as trans-translation. Together, tmRNA and SmpB mimic alanyl-tRNA, entering the A site of stalled ribosomes and accepting transfer of the stalled polypeptide. A portion of tmRNA is then positioned as a template for the ribosome to resume translating. The tmRNA open reading frame encodes a proteolysis tag to mark the aberrant polypeptide for degradation and a stop codon to release the ribosome. How are tmRNA and SmpB allowed into stalled ribosomes? In normal translation, decoding mechanisms carefully monitor the anticodon of tRNAs entering the A site and select only those that are complementary to the mRNA codon. How do tmRNA and SmpB get around the decoding machinery? It appears that interactions between the SmpB C-terminal tail and the decoding center are responsible. Using an in vivo tagging assay and an in vitro peptidyl-transfer assay, we monitored the effect of mutations in the SmpB tail on trans-translation. We found that mutations in SmpB that prevent helix formation are unable to support peptidyl transfer. We also found that while mutation of key nucleotides in the ribosomal decoding center severely inhibit peptidyl transfer to normal tRNAs, these mutations do not inhibit peptidyl transfer to tmRNA. We conclude that the SmpB tail stimulates peptidyl transfer by forming a helix that interacts with the ribosome to signal decoding in a novel manner. How is the tmRNA open reading frame positioned for the ribosome to resume translating? Mutation of the tmRNA nucleotide A86 alters reading frame selection. Using a genetic selection, we identified SmpB mutants that restore normal frame selection to A86C tmRNA without altering frame selection on wild-type tmRNA. Through rational mutation of the SmpB tail we identified an SmpB mutant that supports peptidyl transfer but prevents translation of the tmRNA open reading frame. We conclude that SmpB plays a functional role in selecting the tmRNA open reading frame.

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Watts, Talina Christensen. "Genetic analysis of the role of SmpB in determining frame on tmRNA /". Diss., CLICK HERE for online access, 2008. http://contentdm.lib.byu.edu/ETD/image/etd2501.pdf.

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Watts, Talina Christensen. "Genetic Analysis of the Role of SmpB in Establishing the Reading Frame on tmRNA". BYU ScholarsArchive, 2008. https://scholarsarchive.byu.edu/etd/1728.

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Ribosomes translate the genetic information encoded by mRNA into proteins. Defective mRNAs can cause stalling of translating ribosomes. The molecule tmRNA (transfer-messenger RNA) rescues stalled ribosomes in eubacteria. Together with its protein partner SmpB, tmRNA mimics a tRNA by entering the ribosomal A site and linking an alanine residue to the growing polypeptide chain. The ribosome then abandons the defective mRNA template and resumes translation on tmRNA, adding ten more amino acids to the nascent polypeptide. As a result of tmRNA action, stalled ribosomes are released and recycled, the defective mRNA is destroyed, and the aborted protein product is tagged for destruction by proteases. It is unknown how the ribosome correctly chooses the position on tmRNA to resume translation. Previous studies implicate the sequence UAGUC found immediately upstream of the first codon in the tmRNA open reading frame. These nucleotides are highly conserved in natural tmRNA sequences. Mutations in this area cause loss of tmRNA function and improper frame choice. Using a genetic selection that ties the life of E. coli cells to the function of tmRNA, we have identified several SmpB mutants that rescue an inactive tmRNA in which this upstream sequence was altered. This links SmpB to the function of these key tmRNA nucleotides. We show that our SmpB mutants affect frame choice using an in vivo assay for tagging in the various frames. We conclude that SmpB plays a role in setting the reading frame on tmRNA.

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Guyomar, Charlotte. "Études structurales de la trans-traduction, cible privilégiée pour le développement de nouveaux antibiotiques". Thesis, Rennes 1, 2018. http://www.theses.fr/2018REN1B039.

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Le travail retranscrit dans cette thèse porte sur un processus biologique impliqué dans le contrôle qualité de la synthèse protéique bactérienne : la trans-traduction. Ce processus permet de libérer les ribosomes bloqués sur des ARNm défectueux tout en détruisant les peptides et ARNm problématiques impliqués dans le blocage. Il nécessite deux acteurs principaux qui interagissent avec le ribosome: l’ARN transfert-messager (ARNtm) et la protéine SmpB. Dans un premier chapitre, une étude en cryo-microscopie électronique à transmission (cryo-MET) a permis d’obtenir deux structures à l’échelle atomique impliquant le ribosome et différents acteurs de la trans-traduction. La première structure met en évidence l’interaction entre la RNase R, enzyme responsable de la destruction des ARNm défectueux, et le ribosome bactérien. La deuxième structure a mené à la caractérisation des deux premiers états de la trans-traduction à une résolution quasi-atomique. De nouvelles interactions sont notamment observées entre la protéine SmpB et l’hélice H5 de l’ARNtm. Dans un second chapitre, la trans-traduction est exploitée comme cible pour le développement de nouveaux antibiotiques. En effet, cette voie de sauvetage est souvent vitale ou alors indispensable à la virulence bactérienne. Dans l’objectif de découvrir de nouvelles molécules antibiotiques inhibant la trans-traduction, nous avons mis au point un système de détection de la trans-traduction in vitro. Ce système est simple et rapide, basé sur la mesure de la fluorescence d’une GFP tronquée, réassemblée par un ARNtm muté. La validation du système a conduit à la détection de nouveaux composés anti-trans-traduction
This work is focused on a biological process which controls bacterial protein synthesis, trans-translation. This all-in-one process allows the rescuing of ribosomes stalled on defective mRNA, the degradation of the problematic peptides and mRNA. It is driven by two principal actors that interact with the ribosome: transfer-messenger RNA (tmRNA) and Small protein B (SmpB). In a first chapter, by a cryo-electron microscopic (cryo-EM) study, two near-atomic resolution structures, involving the ribosome and various trans-translation actors, were obtained. The first one highlights the interactions between RNase R, an enzyme responsible for mRNA degradation during trans-translation, and the bacterial ribosome. The second one corresponds to the characterization of two early trans-translation states at a near-atomic resolution. New interactions have been observed between SmpB and tmRNA H5 helix. In a second chapter, trans-translation is used as a target for the development of new antibiotic molecules. Indeed, this pathway is often necessary for bacterial survival and pathogenicity. Towards this aim, we designed and set up a new in vitro assay for high-throughput screening assays. This efficient system is based on fluorescence measurements of a GFP reassembled through trans-translation by a mutated tmRNA. This system has been validated and will be used for the discovery of new anti-trans-translation compounds

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Ivanova, Natalia. "Finding the unknowns in trans-translation". Doctoral thesis, Uppsala : Acta Universitatis Upsaliensis : Univ-bib. [distributör], 2005. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-5756.

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Ranaei-Siadat, Seyed-Ehsan. "Structure, stabilité et interactions de l’ARNtm avant liaison au ribosome". Thesis, Paris 5, 2013. http://www.theses.fr/2013PA05P605.

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Crandall, Jacob N. "Ribosomal RNA Mutations that Inhibit the Activity of Transfer-Messenger RNA of Stalled Ribosomes". Diss., CLICK HERE for online access, 2010. http://contentdm.lib.byu.edu/ETD/image/etd3535.pdf.

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Macé, Kévin. "Le contrôle qualité de la synthèse protéique comme cible pour le développement de nouveaux antibiotiques". Thesis, Rennes 1, 2016. http://www.theses.fr/2016REN1B034/document.

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Le travail retranscrit dans cette thèse regroupe l'étude de différents processus biologiques impliqués dans la synthèse protéique bactérienne. Dans un premier chapitre, les origines de la synthèse protéique au temps du monde ARN sont traitées en guise d'introduction. Ce travail théorique se poursuit par la présentation d'une structure à haute résolution du facteur d'élongation G (EF-G) en complexe avec le ribosome par cryo-microscopie électronique à transmission (cryo-MET). Grâce aux avancées techniques de la cryo-MET, nous avons observé pour la première fois EF-G lié au ribosome en l'absence de tout inhibiteur. Cet état particulièr d'EF-G permet de visualiser une flexibilité de son doamine III. Cette étude permet aussi de rationaliser le fonctionnement de l'antibiotique acide fusidique. Nous nous sommes ensuite intéressés aux voies de sauvetage de la synthèse protéique et plus particulièrement de la trans-traduction. Ce mécanisme fascinant permet le recyclage des ribosomes bloqués sur un ARN messager défectueux. Cette voie de sauvetage est généralement vitale ou alors indispensable pour la virulence bactérienne. Nous avons réalisé une étude structurale préliminaire de la dégradation de l'ARNm défectueux durant ce processus. Après une revue traitant du sujet, nous présentons une étude de la trans-traduction comme cible pour le développement de nouveaux antibiotiques. Pour cela, nous avons mis au point un système rapporteur avec contrôle interne de l'activité trans-traductionnelle bactérienne. Après avoir mis au point ce système et validé son utilisation, nous l'avons exploité en testant des molécules ciblant la trans-traduction
The current PhD work brings together various studies linked to bacterial protein synthesis. The first chapter is about the origins of protein synthesis at the time of the RNA world. This theoretical work continues with the presentation of a high-resolution structure of the elongation factor G (EF-G) in complex with the ribosome by cryo-electron transmission microscopy (cryo-TEM). We describe for the first time EF-G bound to the ribosome in the absence of any inhibitor. This particular structure of EF-G displays a yet unseen positioning of its third domain, which becomes very flexible. This study helps to understand the way the antibiotic fusidic acid blocks translation. The work then switches to a study of trans-translation, the main rescuing system of stalled ribosomes in bacteria. Trans-translation is generally vital or at least necessary for bacterial virulence. We conducted a preliminary structural study on the way faulty mRNAs are degraded during this process. This is why we present a study of trans-translation as a target for the development of new antibiotics. For this we developed and validated a reporter system for trans-translation, which is used to screen molecules targeting trans-translation

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Książki na temat "SmpB"

Jaya, BP Cipta. Petunjuk teknis keuangan Bantuan Operasional Sekolah: Dilengkapi petunjuk pelaksanaan Bantuan Operasional Sekolah (BOS) SD, SDLB, MI, salafiyah/sekolah agama non Islam setara SD, SMP, SMPLB, MTs., salafiyah/sekolah agama non Islam setara SMP, petunjuk teknis monitoring dan evaluasi, petunjuk pelaksanaan bantuan khusus murid (BKM) untuk SMA, SMK, MA & SMLB. Jakarta: Cipta Jaya, 2005.

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Project, School Mathematics. SMP 7-13. Cambridge: Cambridge University Press, 1986.

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Godfrey, Donald G. Founding the Society of Motion Picture and Television Engineers. University of Illinois Press, 2017. http://dx.doi.org/10.5406/illinois/9780252038280.003.0006.

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This chapter focuses on the Society of Motion Picture and Television Engineers (SMPTE), an organization founded by C. Francis Jenkins in 1916. The formation of the SMPTE (formerly Society of Motion Picture Engineers, SMPE) was one of Jenkins' most significant and lasting contributions in the film and television industries. In less than four decades, the SMPTE has evolved into an international association with industry, technological, and creative influence around the world. This chapter provides an overview of the atmosphere that led Jenkins to establish the SMPTE, whose sole purpose would be a discussion of technology and its standards. It also considers the first SMPE meeting and how its influence grew since then, along with its final meeting under Jenkins as president, held in Cleveland in November 1918. Finally, it cites the accolades and foundations established for the SMPE and their impact on the industries of motion pictures and later television engineering.

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SMP. Cambridge: CUP, 1992.

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Inc, AST Research, red. AST Manhattan SMP planning guide: High-performance, extended industry standard architecture (EISA), symmetrical multiprocessor computer. Irvine, CA (16215 Alton Pkwy., Irvine 92713): AST Computer, 1992.

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AST Manhattan SMP technical reference: Advanced, Extended Industry, Standard Architecture (EISA), symmetrical multiprocessor computer. Irvine, CA (P.O. Box 19l658, Irvine 92713-9658): AST Research Inc, 1992.

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SMB Consulting Best Practices (Smb Series). Hara Publishing Group, 2003.

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Project, School Mathematics, red. SMP interact. Cambridge: Cambridge University Press, 2000.

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Project, School Mathematics, red. SMP interact. Cambridge: Camb.U.P., 2000.

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SMB Consulting Best Practices (Harry Brelsford's SMB). Hara Publishing Group, 2003.

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Części książek na temat "SmpB"

Someya, Tatsuhiko, Nobukazu Nameki i Gota Kawai. "SmpB". W Encyclopedia of Systems Biology, 1959. New York, NY: Springer New York, 2013. http://dx.doi.org/10.1007/978-1-4419-9863-7_1322.

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Smith, Roderick W. "Understanding SMB/CIFS". W The Definitive Guide to Samba 3, 3–34. Berkeley, CA: Apress, 2004. http://dx.doi.org/10.1007/978-1-4302-0683-5_1.

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Smet, Carl de, i Witold M. Sokolowski. "SMPU Vane for Femto Satellites". W Cold Hibernated Elastic Memory Structure, 249–58. First edition. | Boca Raton, FL : CRC Press/Taylor & Francis Group, 2018.: CRC Press, 2018. http://dx.doi.org/10.1201/9780429425950-28.

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Thacker, Robert, Hugh Couchman i Frazer Pearce. "Simulating Galaxy Formation on SMPS". W High Performance Computing Systems and Applications, 71–82. Boston, MA: Springer US, 1998. http://dx.doi.org/10.1007/978-1-4615-5611-4_10.

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Smith, Roderick W. "Using SMB/CIFS Clients". W The Definitive Guide to Samba 3, 511–48. Berkeley, CA: Apress, 2004. http://dx.doi.org/10.1007/978-1-4302-0683-5_18.

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Smith, Roderick W. "Samba and SMB/CIFS". W The Definitive Guide to Samba 3, 35–64. Berkeley, CA: Apress, 2004. http://dx.doi.org/10.1007/978-1-4302-0683-5_2.

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Greif, J. M. "The SMP pattern matcher". W EUROCAL '85, 303–14. Berlin, Heidelberg: Springer Berlin Heidelberg, 1985. http://dx.doi.org/10.1007/3-540-15984-3_281.

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Zeng, Guohua, i Wei Zhu. "Super-Mini-PCNL (SMP)". W Percutaneous Nephrolithotomy, 131–36. Singapore: Springer Singapore, 2020. http://dx.doi.org/10.1007/978-981-15-0575-1_15.

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Nahler, Gerhard. "summary of product characteristics (SPC, SmPC)". W Dictionary of Pharmaceutical Medicine, 178. Vienna: Springer Vienna, 2009. http://dx.doi.org/10.1007/978-3-211-89836-9_1362.

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Čejka, Rudolf, Jiří Staroba i Václav Dvořák. "Predicting Performance of SMP Clusters". W Distributed and Parallel Systems, 38–45. Boston, MA: Springer US, 2002. http://dx.doi.org/10.1007/978-1-4615-1167-0_5.

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Streszczenia konferencji na temat "SmpB"

Chat, Sophie. "Deciphering trans-translation by cryo-electron microscopy: motions and interactions of tmRNA and SmpB inside the ribosome". W European Microscopy Congress 2020. Royal Microscopical Society, 2021. http://dx.doi.org/10.22443/rms.emc2020.649.

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Park, Jungkyu, Leon M. Headings, Marcelo J. Dapino, Jeffery W. Baur i Gyaneshwar P. Tandon. "Analysis of Shape Memory Polymer-Alloy Composites: Modeling and Parametric Studies". W ASME 2012 Conference on Smart Materials, Adaptive Structures and Intelligent Systems. American Society of Mechanical Engineers, 2012. http://dx.doi.org/10.1115/smasis2012-8257.

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Shape memory composites (SMCs) based on shape memory alloys (SMAs) and shape memory polymers (SMPs) are interesting due to their controllable temperature-dependent mechanical properties. The complementary characteristics of SMAs and SMPs can be used to create materials or systems with shape recovery created by the SMA and shape fixity provided by the SMP. In this research, three SMC operating regimes are identified and the behavior of SMC structures is analyzed by focusing on composite fixity and interfacial stresses. Analytical models show that certain SMPs can achieve sufficient shape fixing. COMSOL Multi-Physics simulations are in agreement with analytical expressions for shape fixity and interfacial stresses. Analytical models are developed for an end-coupled linear SMP-SMA two-way actuation system.

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Shen, He, Yunjun Xu, Fei Liang, Jihua Gou i Bob Mabbott. "Modeling of Conductive Shape Memory Polymer Nanocomposites Based Structure From a Control Perspective". W ASME 2013 Conference on Smart Materials, Adaptive Structures and Intelligent Systems. American Society of Mechanical Engineers, 2013. http://dx.doi.org/10.1115/smasis2013-3129.

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Shape memory polymers (SMPs) can recover their original shape under external stimulus such as light, heat, pH, humidity, and electric power. However, the applications of SMPs are limited by the number of shapes they can memorize and whether or not these shapes can be precisely and repeatedly controlled. Although a vision based PID controller has been shown by the authors to be capable of controlling the deflection angle of a SMP structure, the repeatability and precision are still low. In order to enhance the robustness and repeatability of the SMP shape control system, in this paper, the macro-scale behavior model of the SMP structure from the control perspective is proposed and the unknown parameters are identified using real-time vision, temperature, and resistance signals.

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Korba, Ahmed G., Mohammad M. Megahed, Hany F. Abdalla i Mohamed M. Nassar. "Prediction of Ratchet Boundary for 90-Degree Smooth and Mitred Pipe Bends". W ASME 2012 Pressure Vessels and Piping Conference. American Society of Mechanical Engineers, 2012. http://dx.doi.org/10.1115/pvp2012-78673.

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The present paper attempts to predict ratchet boundary for 90-degree mitred and smooth pipe bends subjected to sustained pressure and cyclic in-plane bending. The methodology utilizes a recently published technique known as the “Uniform Modified Yielding” (UMY) technique, which relies on generation of a virtual structure with inhomogeneous reduced yield strength, whose magnitude and distribution depend on the elastic stress field due to the cyclic load. The collapse load of this virtual structure determines the threshold steady load necessary for commencement of “incremental collapse”. The technique is applied first to predict ratchet boundaries for two benchmark problems possessing analytical descriptions of ratchet boundary and uni-axial states of stress; the two-bar structure problem and the Bree cylinder. Predicted ratchet boundaries exactly coincided with the corresponding published analytical descriptions, and reasons for this correlation were discussed in this paper. The technique was then applied to three 90-degree pipe bends with similar geometries as follows: smooth pipe bend (SPB), single mitred pipe bend (SMPB), and three weld mitred pipe bend (3WMPB). Certain assumptions are adopted to enable treatment of the problem as a quasi-uniaxial one. Conservative estimates are obtained for ratchet boundaries in pipe bends that correlates well with elastic shakedown/ratchet boundary of the same problems as predicted by a recently developed non-cyclic direct technique.

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Konh, Bardia, Harold H. Lee, Vincent P. Martin, Vincent Zhao, Daehoon Han, Howon Lee i Parsaoran Hutapea. "Design, Development and Evaluation of a Two Way Actuated Steerable Needle". W ASME 2015 Conference on Smart Materials, Adaptive Structures and Intelligent Systems. American Society of Mechanical Engineers, 2015. http://dx.doi.org/10.1115/smasis2015-9084.

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Over the last two decades, researchers have been interested to provide actuation and control for surgical needles. It has been recently suggested to utilize shape memory alloy (SMA) wires to provide bending forces to activate the conventional straight needles. In this paper a design of an active needling system has been proposed where actuation forces of SMAs as well as shape memory polymers (SMPs) were incorporated. SMP elements provide two major additional advantages to the design: (i) recovery of the SMP’s plastic deformation by heating the element above its glass transition temperature, and (ii) achieving a higher needle deflection by having a softer stage of SMP at higher temperatures with less amount of actuation force. The feasibility of providing actuation forces using both SMAs and SMPs for the surgical needle was demonstrated in this study.

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Liang, Fei, Jihua Gou, He Shen, Yunjun Xu i Bob Mabbott. "Carbon Fiber Reinforced Shape Memory Nanocomposites Incorporated With Highly Conductive Carbon Nanopaper for Electro Actuation". W ASME 2013 Conference on Smart Materials, Adaptive Structures and Intelligent Systems. American Society of Mechanical Engineers, 2013. http://dx.doi.org/10.1115/smasis2013-3188.

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Shape memory polymers (SMPs) are one of the most popular smart materials due to light weight and high elastic deformation capability. In this study, highly conductive carbon nanofibers paper (CNFP) was coated on the surface of SMP as a conductive layer for electro actuation of SMP. To overcome the drawback of low modulus and low strength of shape memory polymer (SMP), continuous carbon fiber reinforcement was also incorporated with SMP by autoclave processing. The dynamic mechanical analysis (DMA) result showed over 600% increase of storage modulus of SMP by introducing carbon fiber reinforcement. Also, the shape recovery time of SMP has been reduced over 150%, while the recovery ratio of SMP has been improved to 99% by incorporating with carbon fiber reinforcement. Additionally, the mechanical property degradation of SMP composites has been investigated after different electro actuation cycles. After 50 actuation cycles, the decrease of flexural modulus of SMP composites is negligible (< 2%), and the ultimate flexural strength of SMP composites only decreased 25%. The SMP composite shows high strength and modulus, and good durability.

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McClung, A. J. W., G. P. Tandon, K. E. Goecke i J. W. Baur. "Non-Contact Technique for Characterizing Full-Field Surface Deformation of Shape Memory Polymers". W ASME 2010 Conference on Smart Materials, Adaptive Structures and Intelligent Systems. ASMEDC, 2010. http://dx.doi.org/10.1115/smasis2010-3679.

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Thermally-actuated shape memory polymers (SMPs) typically display two phases separated by the glass transition temperature (Tg). At temperatures well below the Tg, the polymer exhibits a relatively high elastic modulus. Well above the Tg the elastic modulus drops by several orders of magnitude. In this high temperature region, SMP materials can achieve strain levels well above 100 %. The complex behavior of SMPs (stiffnesses dropping to the order of 1 GPa and extremely high strain levels) precludes the use of traditional strain gages and low-contact force extensometers. The present study presents a detailed expansion of state-of-the-art thermomechanical testing techniques used to characterize the material behavior of SMPs. An MTS environmental chamber with an observation window allows for non-contact optical measurements during testing. A laser extensometer is used for measurement and active control of axial strain. The upper limit on the strain rate capability of the laser extensometer is established. In addition, the photographic strain measurement method known as digital image correlation (DIC) is incorporated, allowing for full field measurement of axial and transverse strains of SMPs over a range of temperatures and strain rates. The strain measurements of the DIC and laser extensometer are compared to each other as well as to clip-on extensometers and strain gages. The comparisons provide insight into the limitations of the traditional strain measurement systems. A series of tensile tests are performed on a commercial SMP from 25 °C up to temperatures of 130 °C and strain levels above 100 %. The laser extensometer provides a robust method for controlling the strain in the gage section of the samples. In addition, results show that the full field measurements of both the axial and the transverse strain are essential for characterizing the constitutive response of SMPs at room and elevated temperatures.

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Balogun, Olaniyi A., i Changki Mo. "Shape Memory Polymers: Energy Method Superposition Constitutive Modeling". W ASME 2014 Conference on Smart Materials, Adaptive Structures and Intelligent Systems. American Society of Mechanical Engineers, 2014. http://dx.doi.org/10.1115/smasis2014-7430.

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Shape memory polymers (SMPs) have the capacity to stored strain energy under appropriate stimulus and pre-deformation conditions. Temperature is a good stimulus and predominantly used to activate the shape memory effects of SMPs. Complex engineering application use of SMPs are being developed or proposed and it becomes imperative to develop a simple but yet practical constitutive model that will capture the deformation and recovery of SMPs appropriately under different constraints and loading conditions. In this study, a thermo-mechanical constitutive model is developed for SMPs. A four step shape memorization and recovery is considered and a thermo-mechanical energy balance (first principles of thermodynamics) is done on the individual steps. For this study, the four steps considered are a) Pre-loading of the SMP at high temperature b) Constant strain at negative rate of change of temperature c) constraint release and shape fixity at low temperate and d) unconstrained free strain recovery. A free energy function is developed for the individual steps and superposition principle is used to define the storage free energy in the third step. Applying the second law of thermodynamics in Clausius-Duhem form, the stress-strain relation was developed. Also, in order to account for the polymer’s molecular architecture and morphology resulting in shape memory effect, a binding factor was approximately defined. The binding factor is primarily a function of temperature and secondarily a function of the viscosity of the material at high and low temperatures. The storage strain was assumed to be an internal variable that is generated from the mechanical loading of the SMP. The general model is reduced down to a specific viscoelastic model based on the assumption of the free energy function. The developed model is validated by comparing the predictions to experimental results in literature.

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Bhargava, Aarushi, Kaiyuan Peng, Jerry Stieg, Reza Mirzaeifar i Shima Shahab. "Ultrasound Actuation of Shape-Memory Polymer Filaments: Acoustic-Thermoelastic Modeling and Testing". W ASME 2017 Conference on Smart Materials, Adaptive Structures and Intelligent Systems. American Society of Mechanical Engineers, 2017. http://dx.doi.org/10.1115/smasis2017-3832.

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Controlled drug delivery (CDD) technology has received extensive attention in the past three decades due to numerous advantages of this technology when compared to the conventional methods. Despite recent efforts and substantial achievements, controlled drug releasing systems still face major challenges in practice, including chemical issues with synthesizing biocompatible drug containers and releasing the pharmaceutical compounds at the targeted location with a controlled time rate. In this work, we present experimentally-validated acoustic-thermoelastic mathematical modeling to show the feasibility of using shape memory polymers (SMPs) and focused ultrasound (FU) technology for designing a novel drug-delivery system. SMPs represent a new class of materials that have the ability of storing a temporary shape and returning to their permanent or original shape when subjected to external stimuli such as heat. FU is used as a trigger for noninvasively stimulating SMP-based drug capsules. FU has a superior capability to localize the heating effect, thus initiating the shape recovery process only in selected parts of the polymer. A multiphysics model is developed, which optimizes the design of a SMP-based CDD system using acoustic-thermoelastic analysis of a filament as the constituting base structure and quantifies its activation through FU. The analytical and numerical models are divided into three parts. The first part studies the acoustic behavior of SMPs using Khokhlov-Zabolotskaya-Kuznetsov (KZK) model. The equation solves for acoustic pressure field in a hybrid time-frequency domain using operator-splitting method and examines the effects of absorption, diffraction and nonlinear distortion on the propagating wave in the medium. The second part provides a numerical model based on Penne’s Bioheat equation to estimate the thermal field developed in SMPs as a result of focused acoustic pressure field. The third part provides a numerical framework to predict the mechanical stresses developed in SMPs under FU and consequent shape recovery. The mechanical model is formulated by a compressible neo-Hookean constitutive equation, which assumes the SMPs behave as a thermoelastic material and predicts the shape memory effect under FU. Experimental validation is performed using a FU transducer in a water tank. The recovery of thermally responsive SMPs under FU predicted by our model shows a good accordance with the experiments. The modeling results are used to optimize parameters such as nonlinear properties, input frequency, source power and dimensional effects to achieve maximum shape recovery.

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Herath, Madhubhashitha, Mainul Islam, Jayantha Epaarachchi, Fenghua Zhang i Jinsong Leng. "4D Printed Shape Memory Polymer Composite Structures for Deployable Small Spacecrafts". W ASME 2019 Conference on Smart Materials, Adaptive Structures and Intelligent Systems. American Society of Mechanical Engineers, 2019. http://dx.doi.org/10.1115/smasis2019-5583.

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Abstract Four dimensional (4D) printing is the convergence of three dimensional (3D) printing, which is an emerging additive manufacturing technology for smart materials. 4D printing is referred to the capability of changing the shape, property, or functionality of a 3D printed structure under a particular external stimulus. This paper presents the structural performance, shape memory behavior and photothermal effect of 4D printed pristine shape memory polymer (SMP) and it’s composite (SMPC) with multi-walled carbon nanotubes (MWCNTs). Both materials have demonstrated the ability to retain a temporary shape and then recover their original. It is revealed that the incorporation of MWCNTs into the SMP matrix has enhanced the light stimulus shape recovery capabilities. Light stimulus shape transformation of 4D printed SMPC is advantageous for space engineering applications as light can be focused onto a particular area at a long distance. Subsequently, a model 4D printed deployable boom, which is applicable for small spacecrafts is presented. The shape fixity and recovery behaviors of the proposed boom have been investigated. Notably, the model boom structure has demonstrated ∼86 % shape recovery ratio. The proposed innovative approach of additive manufacturing based deployable composite structures will shape up the future space technologies.

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Raporty organizacyjne na temat "SmpB"

Foley, William Joseph. Operating Envelope Workshop (Beyond SMPs). Office of Scientific and Technical Information (OSTI), lipiec 2019. http://dx.doi.org/10.2172/1532681.

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PARKINSONS INST SUNNYVALE CA. 2008 Strategic Manangement Plan (SMP). Fort Belvoir, VA: Defense Technical Information Center, lipiec 2008. http://dx.doi.org/10.21236/ada485340.

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Pressel, D. M. The Design Constraints for the Memory Systems of Useful SMPs. Fort Belvoir, VA: Defense Technical Information Center, styczeń 2000. http://dx.doi.org/10.21236/ada373394.

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Small, IV, W. LLNL SMP Light Diffuser Fabrication and Preliminary Data. Office of Scientific and Technical Information (OSTI), czerwiec 2006. http://dx.doi.org/10.2172/896295.

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Ustemirov, Nurzhan. Efficient Execution of Electronic Structure Calculations on SMP Clusters. Office of Scientific and Technical Information (OSTI), styczeń 2006. http://dx.doi.org/10.2172/888952.

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Wu, Meng-Shiou. ATCOM: Automatically Tuned Collective Communication System for SMP Clusters. Office of Scientific and Technical Information (OSTI), styczeń 2005. http://dx.doi.org/10.2172/861637.

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Aggarwal, Anshu, i Dirk Grumwald. A Performance Evaluation of the Hemingway DSM System on a Network of SMPs. Fort Belvoir, VA: Defense Technical Information Center, styczeń 1997. http://dx.doi.org/10.21236/ada461990.

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Pressel, D. M., Walter B. Sturek, J. Sahu i K. R. Heavey. How Moderate-Sized RIS C-Based SMPs Can Outperform Much Larger Distributed Memory MPPs. Fort Belvoir, VA: Defense Technical Information Center, październik 1999. http://dx.doi.org/10.21236/ada369805.

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Cheng, Mengdawn, Erik Kabela i Paula Cable-Dunlap. Intercomparison of Aerosol Instrumental Responses Using AEROTRAK, SMPS, and APS in a Simulated Building. Office of Scientific and Technical Information (OSTI), styczeń 2021. http://dx.doi.org/10.2172/1761613.

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Bennett, Nichelle, Marlon D. Crain, Darryl W. Droemer, Raymond Edward Gignac, Gregory A. Lare, Isidro Molina, Rafael Obregon i in. Investigations of shot reproducibility for the SMP diode at 4.5 MV. Office of Scientific and Technical Information (OSTI), listopad 2013. http://dx.doi.org/10.2172/1121975.

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