Artykuły w czasopismach: „SEM”

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CHIANG, YU-CHENG, LI-TUNG CHANG, CHIA-WEI LIN, CHI-YEA YANG i HAU-YANG TSEN. "PCR Primers for the Detection of Staphylococcal Enterotoxins K, L, and M and Survey of Staphylococcal Enterotoxin Types in Staphylococcus aureus Isolates from Food Poisoning Cases in Taiwan". Journal of Food Protection 69, nr 5 (1.05.2006): 1072–79. http://dx.doi.org/10.4315/0362-028x-69.5.1072.

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Staphylococcal enterotoxins (SEs) are important causative agents in gastroenteritidis and food poisoning cases. They are serologically grouped into five major classical types, i.e., SEA, SEB, SEC, SED, and SEE. In addition, new SEs, such as SEG through SEM, have recently been identified and characterized. In an attempt to survey the distribution of classical and new SEs in organisms responsible for staphylococcal infections in Taiwan, we developed PCR primers for the genes that define the SEK, SEL, and SEM types. Bacterial strains other than sek, sel, and sem Staphylococcus aureus, including strains of other Staphylococcus species, did not generate any false-positive results when examined with these primers. The expression potential for the sek, sel, and sem types were also determined by reverse transcription–PCR. Together with the PCR primers specific for the classical SEs and other new SEs, including SEG, SEH, SEI, and SEJ, we surveyed the SE genes in S. aureus strains isolated from food poisoning cases. For 147 S. aureus isolates originating from food poisoning cases, 109 (74.1%) were positive for one or more SE genes. Of them, the major classical enterotoxin type was sea (28.6%), followed by seb (20.4%), sec (8.2%), and sed (2.0%). For the new SE types, sei (30.6%) was detected the most often, followed by sek (18.4%), sem (12.9%), and sel (8.2%). Also, 64 (43.5%) of the total bacterial strains had more than one enterotoxin gene.

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Smyth, Davida S., Patrick J. Hartigan, William J. Meaney, J. Ross Fitzgerald, Claudia F. Deobald, Gregory A. Bohach i Cyril J. Smyth. "Superantigen genes encoded by the egc cluster and SaPIbov are predominant among Staphylococcus aureus isolates from cows, goats, sheep, rabbits and poultry". Journal of Medical Microbiology 54, nr 4 (1.04.2005): 401–11. http://dx.doi.org/10.1099/jmm.0.45863-0.

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In recent years several new staphylococcal enterotoxins (SEs) have been described, which currently have largely unknown frequencies of occurrence and roles in human or animal disease. One hundred and ninety-one Staphylococcus aureus isolates from cows (99), goats (39), sheep (23), rabbits (15), chickens (15) and a cat (1) were screened for SE genes sea–see, seg–seo and seq and for the tst gene encoding staphylococcal toxic shock syndrome toxin-1 using multiplex PCRs and individual PCRs for the seb and sek genes. One hundred and ten isolates tested positive for at least one of these 16 superantigen (SAg)-encoding genes. There were statistically significant differences in the frequencies of some of these SAg genes between isolates from different animals. No strain possessed either the sea or see gene. The sec gene was present in 51 isolates, the sed gene in eight and the seb gene in one. The seh gene was found in four strains and the sek and seq genes together in one isolate. The most common combinations of genes were the egc cluster, bearing the seg, sei, sem, sen and seo genes, in 47 isolates, the sec, sel and tst gene combination typical of the SaPIbov pathogenicity island in 44 isolates, the egc cluster lacking the seg gene in 11 isolates, the sed and sej genes in nine isolates, and the sec and tst genes without the sel gene in seven isolates. The higher frequencies of the sec and tst genes together and the lower frequencies of the egc gene cluster among the SAg gene-positive sheep or goat isolates compared to bovine isolates were statistically significant. Of 36 bovine isolates that were mitogenic for human T lymphocytes, four were negative for the 16 SAg genes tested for, while a further 14 gave borderline results in the mitogenicity assay, 12 of which were SAg gene-negative. Twenty-nine strains lacking all the SAg genes did not induce T-cell proliferation. This survey indicates that novel SE genes seg, sei, sel, sem, sen and seo along with the sec and tst genes predominate in S. aureus from animal hosts. The mitogenicity assays indicate that further uncharacterized SAgs may be present in bovine isolates.

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Sura I. A. Jabuk i Eman M. Jarallah. "Molecular screening of Staphylococcus aureus enterotoxins associated with samples of meat / Iraq". International Journal of Research in Pharmaceutical Sciences 11, nr 4 (17.11.2020): 6685–91. http://dx.doi.org/10.26452/ijrps.v11i4.3590.

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Staphylococcus aureus secreted many types of toxins accompanying Intestinal poisoning resulting from eating food contaminated with bacteria or their toxins. Five hundred meat samples were collected from local markets, including fresh, frozen, canned, sausage and hamburger to investigate their contamination with S.aureus and then determined their ability of these isolates to secrete enterotoxins by using polymerase chain reaction. The results showed that the ratio of isolated S.aureus is 30 (6%) and the percentage of encoding genes for toxins is 30(100%), 0(0%), 3(10), 0(0%),0(0%),3(10), 2(6.7), 1(3.3), 0(0%) and 3(10) to sea, seb, sec, sed, see, seg, see, sei, sej and sel respectively.The result shows the S.aureus isolated from contamination meat able to produce different type to enterotoxins sea, sec, seg, see, sei, and sel and present the sea toxin is the most prevalence type of staphylococcus enterotoxins.

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Ferreira, Daneelly H., Maria das Graças X. Carvalho, Maria J. Nardelli, Francisca G. C. Sousa i Celso J. B. Oliveira. "Occurrence of enterotoxin-encoding genes in Staphylococcus aureus causing mastitis in lactating goats". Pesquisa Veterinária Brasileira 34, nr 7 (lipiec 2014): 633–36. http://dx.doi.org/10.1590/s0100-736x2014000700004.

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Staphylococcal enterotoxins are the leading cause of human food poisoning worldwide. Staphylococcus spp. are the main mastitis-causing agents in goats and frequently found in high counts in goat milk. This study aimed to investigate the occurrence of enterotoxin-encoding genes in Staphylococcus aureus associated with mastitis in lactating goats in Paraiba State, Brazil. Milk samples (n=2024) were collected from 393 farms. Staphylococcus aureus was isolated in 55 milk samples. Classical (sea, seb, sec, sed, see) and novel (seg, seh, sei) enterotoxin-encoding genes were investigated by means of polymerase chain reaction (PCR). From thirty-six tested isolates, enterotoxin-encoding genes were detected in 7 (19.5%) S. aureus. The gene encoding enterotoxin C (seC) was identified in six isolates, while seiwas observed in only one isolate. The genes sea, seb, sed, see, seg and seh were not observed amongst the S. aureus investigated in this study. In summary, S. aureus causing mastitis in goats can harbor enterotoxin-encoding genes and seC was the most frequent gene observed amongst the investigated isolates. This finding is important for surveillance purposes, since enterotoxin C should be investigated in human staphylococcal food poisoning outbreaks caused by consumption of goat milk and dairy products.

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Carvalho, Flávio. "SEM FOCO". Nanocell News 2, nr 9 (16.03.2015): n/a. http://dx.doi.org/10.15729/nanocellnews.2015.03.16.007.

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Eger, Rita de Cássia Schipmann. "SEM TELA, SEM PINCEL, SEM TINTA". Revista Nupeart 7, nr 7 (30.11.2018): 63–72. http://dx.doi.org/10.5965/2358092507072009063.

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Hu, Dong-Liang, Katsuhiko Omoe, Yu Shimoda, Akio Nakane i Kunihiro Shinagawa. "Induction of Emetic Response to Staphylococcal Enterotoxins in the House Musk Shrew (Suncus murinus)". Infection and Immunity 71, nr 1 (styczeń 2003): 567–70. http://dx.doi.org/10.1128/iai.71.1.567-570.2003.

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ABSTRACT The emetic responses induced by staphylococcal enterotoxin A (SEA), SEB, SEC2, SED, SEE, SEG, SEH, and SEI in the house musk shrew (Suncus murinus) were investigated. SEA, SEE, and SEI showed higher emetic activity in the house musk shrew than the other SEs. SEB, SEC2, SED, SEG, and SEH also induced emetic responses in this animal model but relatively high doses were required. The house musk shrew appears to be a valuable model for studying the mechanisms of emetic reactions caused by SEs.

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Kahraman, Beren Basaran, Recep Geckinli i Seyyal Ak. "Detection of enterotoxigenic Staphylococcus aureus in raw and pasteurized milk". Veterinarski arhiv 94, nr 1 (9.02.2024): 33–42. http://dx.doi.org/10.24099/vet.arhiv.2038.

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The aim of this study was to investigate staphylococcal enterotoxins (SEs) by ELISA, and detect the five classical sea, seb, sec, sed, and see genes by real-time PCR in Staphylococcus aureus isolates from raw and pasteurized milk samples. Staphylococcus spp. were isolated from 98 out of 100 raw milk samples, and in 6 out of 100 pasteurized milk samples. On further biochemical tests, S. aureus was isolated in 48 samples (48%) of raw milk (n=100) and in one sample (1%) of pasteurized milk (n=100). Ten (10%) out of 100 raw milk samples were positive for at least one enterotoxin, and the most frequently observed SE was SEA (10%), followed by SEE (7%) and SEB (6%), but none of the isolates were positive for SEC and SED. At least one of the SEs gene types (sea, seb, sec, sed, see) was detected in 45 (93.8%; 45/48) S. aureus isolates from raw milk samples. sec, sea, seb, sed, and see genes were observed in 56.2%, 39.5%, 31.2%, 29.1% and 14.5% of strains respectively. The enterotoxin genes were the single type in 21 (46.7%) of the 45 isolates, there were two in 15 (33.3%), three in six (13.3%), four in two (4.4%), and one (2.2%) in all gene regions. The SE gene was not detected in the S. aureus (n=1) isolate from pasteurized milk. As a result of this study, the presence of enterotoxigenic S. aureus in raw milk was revealed, and it was pointed out that these SEs may contribute to cases of staphylococcal foodborne poisoning (SPF).

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Anselmo da Silva, Bruno, i Karla Regina Macena Pereira Patriota. "Sem glúten, sem lactose e sem vacas". Culturas Midiáticas 13, nr 1 (30.09.2020): 209–27. http://dx.doi.org/10.22478/ufpb.1983-5930.2020v13n1.51035.

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Partindo da análise da materialidade discursiva, constitutiva da campanha publicitária “Atualize o seu leite”, da marca carioca de bebidas naturais Do Bem e nas diversas reações de consumidores a respeito da mesma, sobretudo na rede social digital Instagram, o presente artigo busca demonstrar como determinados discursos em torno da alimentação configuram-se, eles mesmos, como manifestações da ética contemporânea que respalda e legitima o consumo alimentar no Brasil atual. A observação da polêmica em torno da campanha evidenciou as disputas de sentido e lutas por reconhecimento entre o discurso vegano e o do agronegócio, apontando caminhos para a compreensão dos critérios definidores de certo e errado na sociedade brasileira hoje. Palavras-chave: Ativismo. Comida. Consumo. Discurso. Veganismo.

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Holanda Oliveira, Sindy Gabrielly. "Sem vacina, sem impostos e sem mulçumanos". Desenvolvimento Socioeconômico em Debateㅤㅤㅤㅤㅤㅤㅤㅤㅤㅤㅤㅤㅤㅤㅤㅤㅤㅤㅤㅤㅤㅤㅤㅤㅤㅤㅤㅤㅤㅤㅤㅤㅤㅤㅤㅤㅤㅤㅤㅤㅤㅤㅤ 9, nr 1 (6.06.2023): 95–110. http://dx.doi.org/10.18616/rdsd.v9i1.7851.

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O artigo é resultado de uma investigação exploratória, constituído por uma análise acerca do caráter das recentes migrações alemãs para as Colônias Unidas no Paraguai. Centrando-se na discussão das levas migratórias iniciadas no ano de 2021 e que tem continuidade na atualidade, recorre-se a investigação histórica das formações de colônias alemães no país para maior compreensão do fenômeno, o artigo objetiva demonstrar o caráter particular dessas migrações. A particularidade das migrações é evidenciada a priori pelo seu caráter de contrafluxo, uma vez que a maioria das migrações atuais se dirigem aos países da União Europeia, a Alemanha em especial é o país que mais recebe imigrantes e refugiados, tais sujeitos saem de seu país em direção ao interior da região norte do Paraguai. Dentre os fatores mais notórios da decisão migratória desses sujeitos, relatados por eles e que é objeto de reflexão desse artigo, é exatamente um outro fluxo migratório: a chegada expressiva de mulçumanos no território germânico que é vista como um perigo para tais alemães. Além deste motivo, um outro fator destacado pelos migrantes é a suposta arbitrariedade do governo alemão, principalmente em relação a política de vacinação, tais sujeitos chegaram e chegam ao Paraguai sem estarem imunizados contra a COVID-19. Uma outra motivação, desta vez destacada pelos paraguaios, é que para os alemães o país se apresenta mais viável economicamente levando em consideração os valores dos impostos paraguaios em comparação aos alemães.

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Wu, Dejing, Xiangmei Li, Yonghong Yang, Yaojie Zheng, Chuanqing Wang, Li Deng, Lan Liu i in. "Superantigen gene profiles and presence of exfoliative toxin genes in community-acquired meticillin-resistant Staphylococcus aureus isolated from Chinese children". Journal of Medical Microbiology 60, nr 1 (1.01.2011): 35–45. http://dx.doi.org/10.1099/jmm.0.023465-0.

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This study aimed to evaluate the distribution of superantigen gene profiles and the presence of exfoliative toxin genes in community-acquired meticillin-resistant Staphylococcus aureus (CA-MRSA) isolated from Chinese children, and simultaneously to assess virulence gene profiles and genetic background. Of the CA-MRSA isolates, 88.9 % (88/99) harboured toxin genes, with sek as the most frequent toxin gene (62.6 %), followed by seq (61.6 %), seb (60.6 %) and sea (35.4 %). The eta gene was detected only in one ST398-IVa-spa t034 strain. The sed and etd genes were not found in any of the isolates tested. A total of 38 virulence genotypes were observed, of which the genotype seb-sek-seq (27.3 %, 24/88) comprised the majority, followed by sea-seb-sek-seq (18.2 %, 16/88). The enterotoxin gene cluster including seg-sei-sem-sen-seo-seu predominated at a rate of 15.1 %. The relationship among toxin genotypes, toxin genes encoding profiles of mobile genetic elements and genetic background was analysed. Among 66 clonal complex (CC) 59 isolates, 87.9 % (58/66) were positive for toxin genes, and 75.8 % (50/66) harboured the toxin gene combination seb-sek-seq. Among seb-sek-seq-positive CC59 strains, 42.0 % (21/50) also carried the sea gene. CC59 corresponded exclusively to accessory gene regulator 1 (agr-1). The data presented here enhance our current knowledge on the virulence determinants of CA-MRSA.

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Becker, J., K. Rademann i F. Hensel. "Electronic and Geometrical Structure of Se5, Se6, Se7, and Se8". Zeitschrift für Naturforschung A 46, nr 5 (1.05.1991): 453–61. http://dx.doi.org/10.1515/zna-1991-0513.

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AbstractThe vacuum-UV-photoelectron spectra of Se2, Se5, Se6, Se7, and Se8 have been recorded at a photon energy of h ν= 10.0 eV. The isolated molecules are examined in a supersonic molecular beam employing a new photoelectron-photoion coincidence technique. The structure of the photoelectron spectra of selenium molecules with even and odd numbers of atoms differs in a characteristic manner. While the spectra of Se6 and Se8 show one single broad band, three separated bands with different intensities are observed for Se5 and two for Se7. The spectra are compared to molecular orbital energy calculations based on theoretically supposed geometries. The comparison indicates that Se6 and Se8 have Dnd-symmetrical ring structures, whereas Se5 and Se7 are C1h-symmetrical rings

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Gao, Fei, Le Wang, Rong Zhao, Yixiong Wang, Yankun Ma, Rulan Yang, Qi Zhang i Chuangyun Wang. "Rational Combination of Selenium Application Rate and Planting Density to Improve Selenium Uptake, Agronomic Traits, and Yield of Dryland Maize". Plants 13, nr 10 (11.05.2024): 1327. http://dx.doi.org/10.3390/plants13101327.

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Exogenous selenium application could effectively improve the selenium absorption of crops affected by different climatic conditions due to changes in the planting environment and planting conditions. We planted maize at planting densities of 67,500 plants ha−1 (D1) and 75,000 plants ha−1 (D2). Five selenium fertilizer gradients of 0 mg m−2 (Se0), 7.5 mg m−2 (Se1), 15.0 mg m−2 (Se2), 22.5 mg m−2 (Se3), and 30.0 mg m−2 (Se4) were applied to investigate the response of the plants to selenium fertilizer application in terms of the gradient selenium absorption and substance accumulation. With the increase in the amount of selenium fertilizer applied, more of the selenium fertilizer will be absorbed and transported from the leaves to the grains, and the selenium content of the grains will gradually increase and exceed the selenium content of leaves. Under the D2 density in 2022, the selenium content of the grains under Se1, Se2, Se3, and Se4 treatments increased by 65.67%, 72.71%, and 250.53%, respectively, compared with that of Se0. A total of 260.55% of the plants showed a gradient of grain > leaf > cob > stalk from the Se2 treatment, and the overall selenium content of the plants increased first and then decreased. Under the D1 density, compared with the Se0, the dry matter mass of the Se1, Se2, Se3, and Se4 treatments significantly improved by 5.84%, 1.49%, and 14.26% in 2021, and significantly improved by 4.84%, 3.50%, and 2.85% in 2022. The 1000-grain weight under Se2, Se3, and Se4 treatments improved by 8.57%, 9.06%, and 15.56% compared to that under the Se0 treatment, and the yield per ha under the Se2, Se3, and Se4 treatments was 18.58%, 9.09%, and 21.42% higher than that under Se0 treatment, respectively. In addition, a reasonable combination of selenium application rate and density could improve the chlorophyll content and stem growth of dryland maize. This lays a foundation for the efficient application of selenium fertilizer and provides an important reference.

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Couto, Gustavo Belisário d'Araújo. "Sem pai, sem mãe". Cadernos de Campo (São Paulo, 1991) 24, nr 24 (17.06.2016): 140. http://dx.doi.org/10.11606/issn.2316-9133.v24i24p140-157.

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Este artigo busca fazer uma análise de um capítulo do romance de JorgeAmado Capitães da Areia à luz das teorias do parentesco. A situação criada naobra entre as personagens Sem-Pernas e Dona Ester suscita uma discussão sobreo papel da criança nas relações familiares e, mais especificamente, nas relaçõesde filiação. Pensando a família a partir da ótica da personagem criança, a filiaçãopode ser entendida pela chave bourdiesiana da estratégia ou pela chave maussianada reciprocidade. Seja pela reciprocidade ou pela estratégia, a inegável agência dacriança na constituição de relações gera reflexões interessantes sobre o que significa ser pai ou mãe. A escrita de Jorge Amado convida a pensar as categorias mãe e pai menos como um ser e mais como um tornar-se.

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Naffa, Randa G., Salwa M. Bdour, Hussein M. Migdadi i Asem A. Shehabi. "Enterotoxicity and genetic variation among clinical Staphylococcus aureus isolates in Jordan". Journal of Medical Microbiology 55, nr 2 (1.02.2006): 183–87. http://dx.doi.org/10.1099/jmm.0.46183-0.

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A total of 100 Jordanian clinical Staphylococcus aureus isolates was analysed for the presence of the enterotoxin genes sea, seb, sec, sed and see using multiplex PCR. Twenty-three isolates (23 %) were potentially enterotoxigenic. The prevalence of sea, sec and sea plus sec among the total clinical isolates was 15, 4 and 4 %, respectively. None of the isolates harboured sed, seb or see genes. S. aureus isolates were subjected to DNA fingerprinting by randomly amplified polymorphic DNA (RAPD) analysis to test whether isolates harbouring the toxin genes were genetically clustered. A total of 13 genotypes was identified at a 47 % similarity level. Genotypes I and V accounted for the largest number of enterotoxigenic isolates (19 %). This study has demonstrated the genetic diversity of Jordanian clinical S. aureus isolates and shown that the presence of the toxin genes is not genotype specific.

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Otterloo, B. F., i J. L. G. Suijker. "Edge effects in SEM and SAM". Ultramicroscopy 27, nr 2 (marzec 1989): 212–13. http://dx.doi.org/10.1016/0304-3991(89)90138-1.

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ADESIYUN, ABIODUN A., IFEDAPO RAJI i VIVIAN YOBE. "Enterotoxigenicity of Staphylococcus aureus from Anterior Nares of Dining Hall Workers". Journal of Food Protection 49, nr 12 (1.12.1986): 955–57. http://dx.doi.org/10.4315/0362-028x-49.12.955.

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The frequency of isolation of enterotoxigenic Staphylococcus aureus in dining hall workers of a Nigerian University was determined. Of a total of 186 workers sampled, 47 (25.3%) were carriers of enterotoxigenic S. aureus in their anterior nares, including 19 (22.4%) of 85 cooks and 11 (23.9%) of 46 stewards. Fifty-five (26.6%) of 207 strains of S. aureus tested produced staphylococcal enterotoxins A (SEA), B (SEB), C (SEC), D (SED) or E (SEE). SEA predominated, with 18 (8.7%) strains elaborating it and representing 32.7% of all enterotoxigenic strains. SEC and SED were produced by 14 (6.8%) and 13 (6.3%) strains, respectively, and 9 (4.3%) strains produced SEB and SEE. It appears that SEA poses the greatest risk to students consuming foods contaminated by S. aureus of nasal origin from these workers.

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Henrique de Souza Ferraz, Adriano. "DE UM BERGSONISMO SEM DURAÇÃO, SEM MEMÓRIA, SEM ÉLAN". Revista Dissertatio de Filosofia 58 (13.03.2024): 198–217. http://dx.doi.org/10.15210/dissertatio.v58i.26545.

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Na defesa que Maurice Blanchot faz da consistência ontológica da experiência poéticoliterária, surge uma relação filosófica ambígua com bergsonismo. Compreender como o pensamento deHenri Bergson incide em sua teoria é imprescindível para podermos estabelecer a importância deMallarmé na constituição de uma teoria do espeço literário e encontrarmos o devido lugar do devir e daduração nesta teoria da literatura. Em Blanchot podemos verificar algumas linhas de ruptura econtinuidade com o bergsonismo, procedendo sobretudo uma reversão do fluxo temporal no espaçodiferencial da escrita e da própria experiência subjetiva. Não deixamos de notar que Blanchot, ao seguiruma tendência interna de divergência do bergsonismo com ele próprio, recupera um outro lado daexperiência das “ilusões idealistas das representações simbólicas”. Tais ilusões, combatidas porBergson em sua crítica ao conceito e apelo empírico à intuição, formam para Blanchot um substratopositivo-ontológico da linguagem e sobretudo da literatura

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Hait, Jennifer M., Angela T. Nguyen i Sandra M. Tallent. "Analysis of the VIDAS® Staph Enterotoxin III (SET3) for Detection of Staphylococcal Enterotoxins G, H, and I in Foods". Journal of AOAC INTERNATIONAL 101, nr 5 (1.09.2018): 1482–89. http://dx.doi.org/10.5740/jaoacint.17-0501.

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Abstract Background: Staphylococcal food poisoning (SFP) frequently causes illnesses worldwide. SFP occurs from the ingestion of staphylococcal enterotoxins (SEs) preformed in foods by enterotoxigenic strains of Staphylococcus species, primarily S. aureus. SEG, SEH, and SEI induce emesis and have been implicated in outbreaks. Immunological-based methods are deemed the most practical methods for the routine analysis of SEs in foods given their ease of use, sensitivity, specificity, and commercial availability. These kits are routinely used to test for SEA-SEE. However, only recently has a kit been developed to detect SEG, SEH, and SEI. Objective: Our research examined the performance of the novel VIDAS® Staph Enterotoxin III (SET3) for the detection of staphylococcal enterotoxins SEG, SEH, and SEI in foods. Methods: Here we assess the sensitivity and specificity of SET3 using duplicate test portions of six foods at varying concentrations of inclusivity and exclusivity inocula: pure SEG, SEH, SEI, S. aureus strain extracts positive for seg, seh, and sei, as well as SEA, SEB, SEC, SED, and SEE. Results: The overall detection limit was less than 2.09 ng/mL for foods inoculated with SEG, SEH, and SEI, with no cross reactivity observed. Highlights: Integrating concurrent testing to detect the presence of SEA–SEE and SEG–SEI utilizing the SET3 along with the VIDAS SET2, Ridascreen® SET total, or other comparable kits will be instrumental for the future food assessments in our laboratory and may become the new standard for SE analysis of foods.

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Hong, Hyeri, i Alfonso J. Martinez. "Applying SEM, Exploratory SEM, and Bayesian SEM to Personality Assessments". Psych 6, nr 1 (25.01.2024): 111–34. http://dx.doi.org/10.3390/psych6010007.

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Despite the importance of demonstrating and evaluating how structural equation modeling (SEM), exploratory structural equation modeling (ESEM), and Bayesian structural equation modeling (BSEM) work simultaneously, research comparing these analytic techniques is limited with few studies conducted to systematically compare them to each other using correlated-factor, hierarchical, and bifactor models of personality. In this study, we evaluate the performance of SEM, ESEM, and BSEM across correlated-factor, hierarchical, and bifactor structures and multiple estimation techniques (maximum likelihood, robust weighted least squares, and Bayesian estimation) to test the internal structure of personality. Results across correlated-factor, hierarchical, and bifactor models highlighted the importance of controlling for scale coarseness and allowing small off-target loadings when using maximum likelihood (ML) and robust weighted least squares estimation (WLSMV) and including informative priors (IP) when using Bayesian estimation. In general, Bayesian-IP and WLSMV ESEM models provided noticeably best model fits. This study is expected to serve as a guide for professionals and applied researchers, identify the most appropriate ways to represent the structure of personality, and provide templates for future research into personality and other multidimensional representations of psychological constructs. We provide Mplus code for conducting the demonstrated analyses in the online supplement.

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Cebeci, Tugba, Barış Otlu i Elif Seren Tanrıverdi. "Staphylococcus aureus in animal-derived food products: the prevalence, virulence, enterotoxin-encoding genes, antibiotic resistance and PFGE profiles in northern Turkey". Veterinarski arhiv 94, nr 2 (21.03.2024): 141–54. http://dx.doi.org/10.24099/vet.arhiv.2174.

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The aim of this research was to investigate the prevalence of Staphylococcus aureus (S. aureus) in raw milk, Tulum cheese, and ground beef samples, and to determine their virulence, enterotoxins, antibiotic resistance, and genetic relatedness. A total of 300 food samples were purchased from public markets within different districts of Giresun, Turkey. Fifty-two (17.3%) of these food samples tested positive for S. aureus isolation. Fifty-two S. aureus isolates were further analyzed for the presence of virulence genes. The virulence genes detected were icaA (9.6%) and icaD (84.6%). Enterotoxin-encoding genes of the sea, seb, sec, sed, see, seg, seh, sei, sej, sek, sel, sem, sen, seo, seq, ser, and seu groups were detected individually or in combination. Of the 52 S. aureus isolates, 1 methicillin-resistant S. aureus strain (1.9%) was isolated as having the mecA. The antibiotic susceptibility test of positive isolates showed resistance to cefoxitin (1.92%), tetracycline (11.5%), erythromycin (3.84%), ciprofloxacin (1.92%), gentamicin (1.92%), and fusidic acid (5.76%). Pulsed-field gel electrophoresis (PFGE) of the 52 isolates revealed 46 PFGE types, with 21 (40.3%) isolates grouped into 7 clusters. Some of the isolates from different districts showed clonal relatedness. The high occurrence of S. aureus strains in these products indicated a potential risk to humans. The results of this study indicated that dairy and meat products could be reservoirs of S. aureus strains that harbor several virulence factors and enterotoxin genes and the presence of these bacteria in foods may be a cause of concern for human health from food poisoning; therefore, hygienic measures and periodic bacteriological controls are necessary in all areas that provide these foods to the public, such as bazaars and butchers, to reduce contamination with foodborne pathogens.

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Ayeni, Funmilola A., Werner Ruppitsch i Franz Allerberger. "Molecular characterization of clonal lineage and staphylococcal toxin genes fromS. aureusin Southern Nigeria". PeerJ 6 (9.07.2018): e5204. http://dx.doi.org/10.7717/peerj.5204.

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BackgroundStaphylococcus aureusis a human colonizer with high potential for virulence, and the spread of the virulent strains from the colonized hosts to non-carriers in the community is on the increase. However, there are few reports on comprehensive analysis of staphylococcal enterotoxin (SE) genes with clonal lineage inS. aureusin Africa. This is essential because of diversity of cultures and habits of the people. This study analyzed spa types and enterotoxin genes inS. aureusstrains previously isolated from the human nostrils, poultry and clinical samples in Southern Nigeria.MethodsForty-sevenS. aureusisolates were obtained from humans nostrils (n = 13), clinical strains (n = 21) and poultry (n = 13) from previous studies in Southern Nigeria. The strains were analyzed formecAgene, selected toxins genes (sea, seb, sec, sed, see, seg, seh, sei, sej, sek, sel, sem, sen, seo, sep, seq, ser, seu)and Panton-Valentine leukocidin (PVL) gene(lukS-PV/lukF-PV)by PCR. Population structures of the strains were detected by Staphylococcal protein A (spa) typing.ResultsTwenty different spa types were obtained with the highest percentages, 17% observed inspatype t091 from clinical, nasal and poultry samples while t069 was the most prevalent spa type in poultry. Two MRSA were only detected in human strains. The poultry strains had the highest occurrence of SE genes (18%) followed by nasal strains (15%) and clinical strains (10%). Eighty-nine percent of all tested isolates harbored at least one SE gene;seowas the most prevalent (34%) followed byseg(30%) andsea(21%), whilesec, seeandsejwere absent in all strains. Spa type t355 was associated withlukS-PV/lukF-PVgene and complete absence of all studied SE.Sea, seq, seb, sekwere associated with spa type t069;seawas associated with t127 whilesepwas associated with spa type t091. There were coexistences ofseo/segandsei/seg.ConclusionsThe higher carriage of staphylococci enterotoxin genes by the nasal and poultryS. aureusstrains suggests a high potential of spread of staphylococcal food poisoning through poultry and healthy carriers in the community. This is the first report of high occurrence of staphylococcal enterotoxins genes in poultry from Nigeria.

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LI, Y. L., Z. R. HUANG, Q. D. ZHONG, C. CHEN i Y. X. XU. "SECM APPLIED FOR RESEARCHING THE MICROSCOPIC MORPHOLOGY AND CORROSION BEHAVIOR OF ENAMEL COATING ON MILD STEEL IN AQUEOUS CHLORIDE SOLUTIONS". Surface Review and Letters 25, nr 05 (lipiec 2018): 1850094. http://dx.doi.org/10.1142/s0218625x18500944.

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The microstructures, morphology features and the corrosion resistance of the different particle diameters of enamel coating were investigated in this paper. All enamel coating samples were immersed in 5[Formula: see text]wt.% NaCl solution. The effects of the diameter of micron-dimension enamel particles on the mild steel were discussed and the corrosion resistance of the different coatings were characterized by potentiodynamic polarization curves, scanning electron microscopy (SEM) and scanning electrochemical microscopy (SECM), the SECM was employed to identify the local reactivity and then estimate where pittings happen. In this paper, six kinds of diameter of enamel particles were sintered at 780[Formula: see text]C on mild steel. The results indicated that the corrosion resistance of the enamel coating immersed in 5[Formula: see text]wt.% NaCl solution improved first and then declined with the decrease of particle size. Used by SEM and SECM, the result showed that as enamel particles become more small, the binding force becomes stronger. However, as the diameter of enamel particles decrease, the uniformity of the coating first improves and then decreases.

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McLAUCHLIN, J., G. L. NARAYANAN, V. MITHANI i G. O'NEILL. "The Detection of Enterotoxins and Toxic Shock Syndrome Toxin Genes in Staphylococcus aureus by Polymerase Chain Reaction". Journal of Food Protection 63, nr 4 (1.04.2000): 479–88. http://dx.doi.org/10.4315/0362-028x-63.4.479.

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A simple polymerase chain reaction (PCR)-based procedure was developed for the detection of fragments of staphylococcal enterotoxins (SEs) SEA, SEB, SEC, SED, SEE, SEG, SEH, and SEI together with the toxic shock syndrome toxin (TSST-1) genes of Staphylococcus aureus. One hundred and twenty-nine cultures of S. aureus were selected, 39 of which were recovered from 38 suspected staphylococcal food-poisoning incidents. The method was reproducible, and 32 different toxin genotypes were recognized. The presence of SE genes was associated with S. aureus strains reacting with phages in group III, and the TSST-1 gene with phages in group I. There was a 96% agreement between the PCR results for detection of SEA–D and TSST-1 as compared with a commercial reverse passive latex agglutination assay for the detection of SEs from cultures grown in vitro. Enterotoxin gene fragments were detected in S. aureus cultures recovered from 32 of the 38 suspected staphylococcal food poisoning incidents, and of these, 17 were associated with SEE, SEG, SEH, and SEI in the absence of SEA–D. Simple PCR procedures were also developed for the detection of SE directly in spiked food samples, and this was most successfully achieved in mushroom soup and ham. Detection was less successful in three types of cheese and in cream. SEA or SEB were detected by enzyme-linked immunosorbent assay in three food samples (two of which were associated with food poisoning incidents) naturally heavily contaminated with S. aureus: the appropriate SEA or SEB gene fragments were detected directly in these three foods by PCR.

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Kot, Barbara, Piotr Szweda, Aneta Frankowska-Maciejewska, Małgorzata Piechota i Katarzyna Wolska. "Virulence gene profiles inStaphylococcus aureusisolated from cows with subclinical mastitis in eastern Poland". Journal of Dairy Research 83, nr 2 (1.04.2016): 228–35. http://dx.doi.org/10.1017/s002202991600008x.

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Staphylococcus aureusis arguably the most important pathogen involved in bovine mastitis. The aim of this study was to determine the virulence gene profiles of 124Staph. aureusisolates from subclinical mastitis in cows in eastern Poland. The presence of 30 virulence genes encoding adhesins, proteases and superantigenic toxins was investigated by PCR. The 17 different combinations of adhesin genes were identified. Occurrence ofeno(91·1%) andfib(82·3%) genes was found to be common. The frequency of other adhesion genesfnbA, fnbB, ebpswere 14·5, 50, 25%, respectively, and forcnaandbbpwere 1·6%. TheetAandetDgenes, encoding exfoliative toxins, were present in genomes of 5·6 and 8·9% isolates, respectively. ThesplAandsspA, encoding serine protease, were detected in above 90% isolates. The most frequent enterotoxin genes weresei(21%),sem(19·4%),sen(19·4%),seg(18·5%) andseo(13·7%). Thetstgene was harboured by 2·4% isolates. The 19 combinations of the superantigenic toxin genes were obtained and found in 35·5% of isolates. Three of them (seg, sei, sem, sen, seo; sec, seg, sei, sem, sen, seoandseg, sei, sem, sen) were the most frequent and found in 16·1% of the isolates. The most common virulotype, present in 17·7% of the isolates, wasfib, eno, fnbB, splA, splE, sspA. The results indicate the variation in the presence of virulence genes inStaph. aureusisolates and considerable diversity of isolates that are able to cause mastitis in cows.

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Batalha, Douglas. "SEM". Revista Debates Insubmissos 6, nr 20 (9.05.2023): 358. http://dx.doi.org/10.32359/debin2023.v6.n20.p358-359.

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Carvalho, Eros Moreira de. "Internismo sem intelectualismo e sem reflexividade". Kriterion: Revista de Filosofia 55, nr 129 (czerwiec 2014): 153–72. http://dx.doi.org/10.1590/s0100-512x2014000100009.

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Em seu livro, "Perception as a Capacity for Knowledge" (2011), John McDowell defende que a garantia fornecida pela percepção é infalível. Para tanto, é preciso entender o papel que a razão tem na constituição de estados perceptivos genuínos. Por meio dela, posicionamos estes estados no espaço lógico das razões. Assim, não só fazemos do estado perceptivo um episódio de conhecimento, mas também obtemos conhecimento de como chegamos a este conhecimento. McDowell sustenta que esta condição para o conhecimento, a posse da capacidade de posicionar um estado perceptivo no espaço lógico das razões, não o compromete com o intelectualismo. Neste artigo, defendo que o internismo de McDowell não está totalmente livre do intelectualismo e que o internismo é mais plausível não só sem intelectualismo, mas também sem reflexividade.

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Assis, Ana Elisa Spaolonzi Queiroz. "Escola sem partido: projeto sem sustância". Revista Exitus 8, nr 2 (1.05.2018): 15. http://dx.doi.org/10.24065/2237-9460.2018v8n2id527.

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No ano de 2017 tive a oportunidade de discutir o Projeto Escola Sem Partido em cinco ocasiões. A primeira foi durante a Semana da Ação Mundial (SAM), organizada pela “Campanha Nacional pelo Direito à Educação” com apoio da Faculdade de Educação da Universidade Estadual de Campinas (FE/UNICAMP) e do Grupo de Estudos e Pesquisas em Política Educacional (GREPPE) coordenado pela Profª. Drª. Theresa Adrião. Reunidos os Profs. Drs. Daniel Cara (Coordenador da Campanha Nacional pelo Direito à Educação), Wencesláo Machado de Oliveira Júnior (professor da FE/UNICAMP e membro, à época, do Conselho Municipal de Educação de Campinas) e eu em mesa intitulada “Direito Humano à Educação e Respeito à Diversidade: Sobre a Mordaça na e da Escola”, parti do pressuposto de que, no Brasil, a SAM não pode discutir rumos ao desenvolvimento sustentável, vinculado à efetivação plena do direito a educação, sem priorizar a democracia como valor imprescindível destes espaços de atuação e formação.

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Griffin, B. J., i A. van Riessen. "Virtual SEM 1.2 - Training in SEM". Proceedings, annual meeting, Electron Microscopy Society of America 54 (11.08.1996): 406–7. http://dx.doi.org/10.1017/s0424820100164490.

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The greatest barrier to effective training in electron microscopy is the initial apprehension within students based on the complexity and cost of the instrumentation. A second, and today more real barrier, is the actual cost of hands-on training in courses, in terms of both instrument and staff resources. The software described below was developed to eliminate these barriers, to include a ‘fun’ component to relax students, to improve the effectiveness of short training courses and to act as a stand-alone resource for new SEM operators.A software package utilising multimedia components, “Virtual SEM 1.2”, has been developed to simulate the use and operation of a basic scanning electron microscope (SEM). Background SEM theory is provided through introductory tutorials and an automatic assessment section allows independent evaluation of student progress. The package is appropriate for entry level operator training and also as a reference for general operation.

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Dias, N. L., D. C. B. Silva, D. C. B. S. Oliveira, A. A. Fonseca Junior, M. L. Sales i N. Silva. "Detecção dos genes de Staphylococcus aureus, enterotoxinas e de resistência à meticilina em leite". Arquivo Brasileiro de Medicina Veterinária e Zootecnia 63, nr 6 (grudzień 2011): 1547–52. http://dx.doi.org/10.1590/s0102-09352011000600036.

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Realizou-se a detecção do gene de Staphylococcus aureus, de enterotoxinas e de resistência à meticilina com extração de DNA feita diretamente de amostras de leite. Das 200 amostras estudadas, 145 (72,5%) amplificaram o gene femA, e estas foram analisadas quanto à presença dos genes sea, seb, sec e mecA. Os genes das enterotoxinas mais prevalentes foram: sea (60%), seb (37,9%) e sec (6,9%). Foram encontradas 18 amostras de leite (11,0 %) com S. aureus portadores do gene mecA. A detecção de S. aureus diretamente do leite, sem a necessidade de isolamento bacteriano e a caracterização do potencial enterotoxigênico, demonstra que a técnica de PCR é muito útil para estudos epidemiológicos das infecções estafilocócicas da glândula mamária. O alto percentual (72,5%) de amostras de leite positivas para a presença do gene femA sugere que S. aureus constitui um dos principais agentes causadores de infecções intramamárias na microrregião de Sete Lagoas-MG e que seu potencial enterotoxigênico e presença do gene mecA, que identifica o S. aureus resistente à meticlina, representa um risco potencial à saúde pública.

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Radoslava, Savić Radovanović, Zdravković Nemanja i Velebit Branko. "Occurrence and Characterization of Enterotoxigenic Staphylococci Isolated from Soft Cheeses in Serbia". Acta Veterinaria 70, nr 2 (1.06.2020): 238–54. http://dx.doi.org/10.2478/acve-2020-0017.

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AbstractA total of 415 cheese samples produced with raw or cooked milk collected from local markets were analysed for the presence of coagulase-positive staphylococci. In 85 (20.48%) samples the presence of coagulase positive staphylococci was detected. The ELFA technique VIDAS SET2 (BioMerieux, France) was used for testing coagulase-positive staphylococci strains to produce classical enterotoxins (SEA, SEB, SEC, SED, SEE), and to determine the enterotoxins in cheese samples. The number of coagulase-positive staphylococci in cheese samples ranged from 1-5.79 log CFU g-1. Out of 85 coagulase-positive strains 26 (30.59%) produced enterotoxins. The presence of genes for the synthesis of staphylococcal enterotoxins (SE) in the obtained extracts of DNA from 26 enterotoxigenic strains was detected by conventional multiplex PCR technique (for genes sea and seb) i.e. the Real-Time PCR technique for genes sec, sed and see. In all 26 strains of coagulase-positive staphylococci (originating from cheeses produced from raw or cooked milk, which were enterotoxin producers) sea was present, and in 24 strains in addition to sea gene, seb was detected. None of the isolates possessed genes for the synthesis of enterotoxin C (SEC), D (SED) and E (SEE). Out of 26 tested cheese samples positive for enterotoxigenic coagulase-positive staphylococci, enterotoxin was detected in 2 (7.69%) samples of sweet-coagulating cheese, in which the number of enterotoxigenic coagulase-positive staphylococci exceeded 5 log CFU g-1. In sweet-coagulating cheeses in which the number of coagulase-positive staphylococci exceeds 5 log CFU g-1 and the pH value was higher than 5.0, enterotoxins may be present in amounts sufficient to cause intoxication.

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Wang, Le, Fei Gao, Liguang Zhang, Li Zhao, Yan Deng, Hongxia Guo, Lixia Qin i Chuangyun Wang. "Effects of Basal Selenium Fertilizer Application on Agronomic Traits, Yield, Quality, and Se Content of Dryland Maize". Plants 11, nr 22 (15.11.2022): 3099. http://dx.doi.org/10.3390/plants11223099.

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To explore the efficiency of selenium (Se) fertilizer application in dryland maize, we tested five Se fertilizer application treatments: 0 g ha−1 (Se0), 75 g ha−1 (Se1), 150 g ha−1 (Se2), 225 g ha−1 (Se3), and 300 g ha−1 (Se4). Compared with Se0, Se2 increased the leaf area, chlorophyll content, internode length, plant height, and ear height by 7.95%, 3.20%, 13.19%, 1.89%, and 7.98%, respectively. Se2 and Se3 significantly affected the stem internode diameter, cortex thickness, and cellulose content, which were positively correlated with lodging resistance. Compared with Se0, Se3 promoted the contents of soluble sugar, crude protein, crude fat, and starch in grains, which increased by 9.48%, 6.59%, 1.56%, and 4.82%, respectively. It implies that maize grain application of Se significantly improves their Se content. Se1 did not observably influence the growth of maize, and the promoting effect of Se4 on maize decreased. The lodging resistance of maize as analyzed by Pearson correlation analysis correlated with the application of Se fertilizer. It proved that higher yield, grain quality, grain Se content, and lodging resistance of stems were concerned with Se fertilizer application in the range of 150–225 g ha−1. The results provide useful information for Se fertilizer treatment in dryland maize.

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Pitcher, Tammy, i Rochelle Buffenstein. "Passive uptake in the small intestine and active uptake in the hindgut contribute to the highly efficient mineral metabolism of the common mole-rat, Cryptomys hottentotus". British Journal of Nutrition 71, nr 4 (kwiecień 1994): 573–82. http://dx.doi.org/10.1079/bjn19940164.

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Cryptomys hottentotus has no access to dietary or endogenous sources of cholecalciferol (D3). Serum concentrations of calcifediol (25(OH)D3) were undetectable (< 5 ng/ml) and calcitriol (1,25(OH)2D3), although detectable in plasma, was at a low concentration (31.40) (SEM 3.06 pg/ml). Despite their naturally impoverished vitamin D status, these animals exhibited highly efficient mineral absorption processes (Ca apparent fractional absorption efficiency, AFA (%) 95.33 (SEM 1.36); inorganic P (Pi) AFA (%) 93.49 (SEM 0.81)). Furthermore, plasma mineral content was tightly regulated (Ca 2.57 (SEM 0.08) mmol/l; Mg 1.23 (SEM 0.05) mmol/l; Pi 2.12 (SEM 0.15) mmol/1). Mode of uptake in C. hottentotus was unlike that in other D3-replete mammals. First, passive (rather than active) uptake occurred in the traditional site of active Ca absorption (with serosal: mucosal (S:M) ratios in the duodenum of 1.32 (SEM 0.13)), and the only site of active Ca uptake was the hindgut (caecum S:M 3.35 (SEM 0.46); proximal colon S:M 2.13 (SEM 0.30)). Despite the presence of active uptake in these hindgut regions, their overall contribution to the daily rate of mineral absorption was small (9.53 (SEM 1.27)%). These underground inhabitants rely upon highly efficient, passive mineral uptake. This is adequate to meet their mineral requirements and maintain mineral homeostasis in the absence of vitamin D.

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Angarita-Merchán, Maritza, i Nuri Andrea Merchán Castellanos. "Genes codificantes para enterotoxinas de aislados estafilocócicos coagulasa negativos y coagulasa-positivos a partir muestras con mastitis bovina". Revista Investigación en Salud Universidad de Boyacá 5, nr 2 (3.09.2018): 205–18. http://dx.doi.org/10.24267/23897325.267.

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Introducción: La mastitis bovina es la inflamación de glándulas mamarias y tejidos secretores. El género Staphylococcus es el agente causal más importante, por su capacidad de producir diferentes factores de virulencia. Las enterotoxinas estafilocócicas son un grupo importante de toxinas que permiten al microorganismo invadir células y tejido huésped, siendo diseminadas a través de productos alimenticios y responsables de graves intoxicaciones alimentarias en el mundo. Objetivo: Determinar la presencia de genes codificantes para enterotoxinas estafilocócicas (SE); SEA, SEB, SEC, SED y SEE, en cepas de Staphylococcus spp. asociados a mastitis bovina. Materiales y Métodos: Estudio cuantitativo, descriptivo y transversal. Se realizó identificación de especie a través de la amplificación de la región r16S. La detección de genes sea, see, sec, sed, y see se realizó mediante la amplificación por PCR convencional, usando iniciadores específicos para cada gen y se evidenciaron los amplicones a través de electroforesis. Resultados: Se evidenció el predominio del grupo Staphylococcus coagulasa positivo (65.2%), siendo Staphylococcus aureus la cepa con mayor presencia (88.5%), mientras que Staphylococcus coagulasa negativo fue del 37.5%. El gen sea fue detectado en Staphylococcus sciuri (1.7%); seb en Staphylococcus pasteuri y Staphylococcus warneri (3.6%); sec fue identificado en Staphylococcus sciuri y Staphylococcus saprophyticus (3.6%); no se detectaron los genes sed y see en ninguna de las cepas evaluadas. Conclusiones: Los resultados apoyan la evidencia que el desarrollo de mastitis bovina también es causado por Staphylococcus Coagulasa Negativa, indicando la posibilidad que este grupo adquiera atributos genéticos como enterotoxinas y factores de virulencia por transferencia horizontal.

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Mattos, Amana Rocha, Ana Maria Bandeira de Mello Magaldi, Carina Martins Costa, Conceição Firmina Seixas Silva, Fernando De Araujo Penna, Luciana Velloso, Paula Leonardi i Verena Alberti. "“Escola sem Partido” ou educação sem liberdade?" Cadernos de Educação Básica 1, nr 2 (20.10.2016): 155. http://dx.doi.org/10.33025/ceb.v1i2.803.

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Albuquerque Junior, Durval Muniz de. "Narrar vidas, sem pudor e sem pecado". albuquerque: revista de história 12, nr 24 (26.12.2020): 12–23. http://dx.doi.org/10.46401/ardh.2020.v12.12157.

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A partir de diálogos estabelecidos com obra Roland Barthes por Roland Barthes (2017), o presente artigo aborda a potência carnal que corporifica a escrita biográfica. Trata-se, então, de refletir sobre a aproximação entre carne e letra, apontando os espaçamentos entre elas, sobretudo no momento de escrever a biografia histórica de uma trajetória que habitou fora do texto. Assim, o debate aqui apresentado se interessa em trazer à tona, para a escrita biográfica, a dimensão espaço temporal e carnal da existência.

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Makita, Takashi, Kazumi Hirabara i Haruko Hirose. "Combination of cryo-SEM and WET-SEM". Proceedings, annual meeting, Electron Microscopy Society of America 45 (sierpień 1987): 568–69. http://dx.doi.org/10.1017/s0424820100127360.

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WET-SEM is a version of commercially available SEM equipped with Robinson's type of wide-angle backscattered electron detector to observe wet samples under low vacuum(0.3-0.5 torr) and it has been used to variable biological samples with or without chemical fixation. Its versatility to observe hydrated specimens without any metalic coating is obviously advantageous to application of cryo-SEM to biological samples.Recent improvement of nitrogen gas cooled cold stage, and vacuum transfer device(Hexland, England) made the WET-SEM(ISI, Akashi, Japan) as a tool for quick survey of unfixed, hydrated, uncoated, and frozen fractured tissue blocks of animals. For examples, tissue from the rat liver or the mice kidney was quickly frozen in nitrogen slush for several minutes and then transfered to prechamber with a type of vacuum transfer system. Within the prechamber the surface of frozen sample is sublimated or fractured under vacuum and then the sample is ready to be seen on the cryo stage which is cooled by nitrogen gas.

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Silva e Sousa, Fernanda. "Sem nomes e sem histórias, mas amados". História da Historiografia: International Journal of Theory and History of Historiography 16, nr 41 (28.06.2023): 1–31. http://dx.doi.org/10.15848/hh.v16i41.1997.

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O artigo se propõe a pensar Perder a mãe: uma jornada pela rota atlântica da escravidão, de Saidiya Hartman, em diálogo com os seus ensaios “Tempo da escravidão” e “Vênus em dois atos”, como uma escrita da história da escravidão que concebe os escravizados como seus ancestrais à medida que a autora se afirma como uma descendente de escravizados que vive a sobrevida da escravidão. Situando-a na nova história social da escravidão e no debate em torno do caráternarrativo e ficcional da história, argumentamos como Hartman, apoiada em uma tradição radical negra, elabora uma escrita da história que tenta dar conta dos milhões de mortos sem nomes e sem histórias ao longo do tráfico de escravos. Nesse processo, não apenas o esquecimento, mas também a impossibilidade de lembrar diante da escassez de fontes documentais ou de contornar a violência do arquivo se impõem como desafios ao trabalho de luto dessas vidas perdidas, levando-a à criação do método da fabulação crítica, que dialoga com o pensamento de Toni Morrison ao escrever o romance Amada. Ao fim, conclui-se que a escrita da história da escravidão deve ser acompanhada por um gesto ético de cuidado com os mortos, buscando não a redenção, mas a narração dessas vidas perdidas.

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Stettler, Stefany. "Mundo-sem-nós e Nós-sem-mundo". Revista Enunciação 9, nr 1 (14.06.2024): 104–21. http://dx.doi.org/10.61378/yeqm7v88.

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The concepts of "world-without-us" and "us-without-world," coined by Eduardo Viveiros de Castro and Déborah Danowski in the work "Há mundo por vir?" (2017), when related to cinematic zombies – creatures challenging Cartesian differentiation between res cogitans and res extensa due to their lack of consciousness, i.e., the inability to recognize the world – prompt the question: what would it be like, borrowing Giorgio Agamben's formulation (2006), if there were neither a world nor humans, without ecosystem and species ceasing to exist? Is it possible to have a world-without-us or us-without-world without the differentiation between Nature and Culture? The hypothesis I intend to explore throughout this text is that, upon investigating the proposed concepts, it may be concluded that contemporary cinematic figures of zombies exist because they mobilize the same dualistic concepts used to differentiate and hierarchize beings that influenced their genesis in the post-colonial folklore of Haiti. To provide an answer to these questions, I propose an investigation into the concepts of "us," "world," and "recognition."

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Suh, Jeong, Hee-Shin Kang i Jeong-O. Kim. "Precision Joining Technology in the SEM". Journal of the Korean Welding and Joining Society 26, nr 4 (31.08.2008): 21–26. http://dx.doi.org/10.5781/kwjs.2008.26.4.021.

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Sauer, P., J. Síla, T. Štosová, R. Večeřová, P. Hejnar, I. Vágnerová, M. Kolář i in. "Prevalence of genes encoding extracellular virulence factors among meticillin-resistant Staphylococcus aureus isolates from the University Hospital, Olomouc, Czech Republic". Journal of Medical Microbiology 57, nr 4 (1.04.2008): 403–10. http://dx.doi.org/10.1099/jmm.0.47413-0.

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A rather fast and complicated progression of an infection caused by some strains of Staphylococcus aureus could be associated with the expression and co-action of virulence factor complexes in these strains. This study screened the antibiotic susceptibility and prevalence of virulence markers in isolates of meticillin-resistant S. aureus (MRSA) obtained from patients hospitalized at the University Hospital in Olomouc, Czech Republic. A total of 100 isolates was screened for 13 genes encoding extracellular virulence determinants (tst, pvl, eta, etb, sea, seb, sec, sed, see, seg, seh, sei and sej) and for their distribution in sample types. Eighty-nine isolates were positive for at least one of the genes. Genes for etb, pvl, see and seh were not detected in any of the MRSA isolates. No statistically significant differences in the occurrence of the determinants studied among sample types were found.

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Cowley, J. M., i J. Liu. "Contrast and resolution in REM, SEM and SAM". Surface Science 298, nr 2-3 (grudzień 1993): 456–67. http://dx.doi.org/10.1016/0039-6028(93)90061-n.

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Homsombat, Theeyathart, Sukolrat Boonyayatra, Nattakarn Awaiwanont i Duangporn Pichpol. "Effect of Temperature on the Expression of Classical Enterotoxin Genes among Staphylococci Associated with Bovine Mastitis". Pathogens 10, nr 8 (2.08.2021): 975. http://dx.doi.org/10.3390/pathogens10080975.

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Staphylococcal food poisoning (SFP), caused by the contamination of staphylococcal enterotoxins, is a common foodborne disease worldwide. The aims of this study were: (1) to investigate classical staphylococcal enterotoxin genes, sea, seb, sec, sed, and see, among Staphylococcus aureus and coagulase-negative staphylococci (CNS) associated with bovine mastitis; (2) to determine the effect of temperature on the expression of classical staphylococcal enterotoxin genes in staphylococci in milk. The detection of classical staphylococcal enterotoxin genes was performed using S. aureus (n = 51) and CNS (n = 47). The expression of classical enterotoxin genes, including sea, seb, sec, and see, was determined during the growth of staphylococci in milk subjected to ultra-high-temperature processing at two different temperatures: 8 °C and room temperature. Classical staphylococcal enterotoxin genes were expressed more frequently in S. aureus (35.30%) than in CNS (12.77%). The sec gene was most frequently detected in S. aureus (29.41%) and CNS (6.38%). Moreover, the expression of sea and sec was significantly higher at room temperature than at 8 °C after 16 h of incubation (p < 0.05). These results emphasize the importance of maintaining the storage temperature of milk below 8 °C to reduce the risk of SFP.

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Kim, Jae Chul, i Jongmin Ra. "A Comparison of the Bayesian SEM and Traditional SEM for the Stability of the Parameter Calibration and the Goodness of Fit Index in the Youth Research". Korean Journal of Youth Studies 25, nr 2 (28.02.2018): 419–45. http://dx.doi.org/10.21509/kjys.2018.02.25.2.419.

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Oliveira, Lorena D`Arc Menezes de. "Sem título". POIÉSIS 18, nr 30 (31.12.2017): 97. http://dx.doi.org/10.22409/poiesis.1830.97-106.

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Nuernberger, Renan. "Sem título". Revista Ensaios 2, nr 4 (10.09.2010): 17. http://dx.doi.org/10.22409/re.v2i4.298.

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Bersani, Humberto, i Letícia Ferreira da Silva. "Sem maquiagem". Cadernos Cemarx, nr 10 (17.01.2018): 269–72. http://dx.doi.org/10.20396/cemarx.v0i10.10929.

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Sem Maquiagem resulta da tese de doutorado de Ludmila Costhek Abílio defendida no Instituto de Filosofi a e Ciências Humanas da Universidade Estadual de Campinas. A autora analisa dados e informações sobre as revendedoras da Natura, não se restringindo à pesquisa de campo realizada com essas trabalhadoras, mas estabelece um diálogo profundo entre sociologia do trabalho e economia política, apresentando os atuais fenômenos no mundo do trabalho sob a perspectiva da condição dessas mulheres.

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De Carvalho, Age. "Sem Clarice". MOARA – Revista Eletrônica do Programa de Pós-Graduação em Letras ISSN: 0104-0944, nr 49 (29.11.2018): 03. http://dx.doi.org/10.18542/moara.v0i49.6262.

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Enap, RSP. "SEM PROTOCOLO". Revista do Serviço Público 43, nr 4 (23.06.2017): 55. http://dx.doi.org/10.21874/rsp.v43i4.2042.

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Enap, RSP. "SEM PROTOCOLO". Revista do Serviço Público 43, nr 7 (12.06.2017): 54–55. http://dx.doi.org/10.21874/rsp.v43i7.1911.

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