Gotowe bibliografie tematyczne / Seegage analysis

Gotowa bibliografia na temat „Seegage analysis”

Autor: Grafiati

Data publikacji: 13 lipca 2024

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Artykuły w czasopismach na temat "Seegage analysis"

Strydom, K., F. Ismail, M. M. Z. Matabane, O. Onwuegbuna, S. V. Omar i N. Ismail. "Comparison of Three Commercial Molecular Assays for Detection of Rifampin and Isoniazid Resistance among Mycobacterium tuberculosis Isolates in a High-HIV-Prevalence Setting: TABLE 1". Journal of Clinical Microbiology 53, nr 9 (1.07.2015): 3032–34. http://dx.doi.org/10.1128/jcm.01691-15.

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In a head-to-head comparison of the MTBDRplusversion 2.0 (Hain Lifescience), the Xpert MTB/RIF (Cepheid), and the Anyplex MTB/NTM (Seegene) assays, we demonstrated equal sensitivity (59/61; 96.7%) and specificity (53/54; 98.1%) for detecting rifampin resistance with further analysis of discordances. The Xpert assay does not detect isoniazid resistance while the Anyplex assay showed high false positivity.

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Pavletić, Martina, Marija Mazor, Mate Lerga, Tatjana Mileta, Jelena Železnjak, Tina Ružić, Sanda Ravlić i in. "Fast, Reliable, and Simple Point-of-Care-like Adaptation of RT-qPCR for the Detection of SARS-CoV-2 for Use in Hospital Emergency Departments". Viruses 13, nr 12 (2.12.2021): 2413. http://dx.doi.org/10.3390/v13122413.

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During COVID-19 pandemics, the availability of testing has often been a limiting factor during patient admissions into the hospital. To circumvent this problem, we adapted an existing diagnostic assay, Seegene Allplex SARS-CoV-2, into a point-of-care-style direct qPCR (POC dqPCR) assay and implemented it in the Emergency Department of Clinical Hospital Center Rijeka, Croatia. In a 4-month analysis, we tested over 10,000 patients and demonstrated that POC-dqPCR is robust and reliable and can be successfully implemented in emergency departments and similar near-patient settings and can be performed by medical personnel with little prior experience in qPCR.

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Lim, Ho-Jae, Jung-Eun Park, Min-Young Park, Joo-Hwan Baek, Sunkyung Jung, Nackmoon Sung, Jae-Hyun Yang, Min-Woo Lee, Sun-Hwa Lee i Yong-Jin Yang. "Assay System for Simultaneous Detection of SARS-CoV-2 and Other Respiratory Viruses". Diagnostics 11, nr 6 (13.06.2021): 1084. http://dx.doi.org/10.3390/diagnostics11061084.

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Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) triggers disease with nonspecific symptoms that overlap those of infections caused by other seasonal respiratory viruses (RVs), such as the influenza virus (Flu) or respiratory syncytial virus (RSV). A molecular assay for accurate and rapid detection of RV and SARS-CoV-2 is crucial to manage these infections. Here, we compared the analytical performance and clinical reliability of Allplex™ SARS-CoV-2/FluA/FluB/RSV (SC2FabR; Seegene Inc., Seoul, South Korea) kit with those of four commercially available RV detection kits. Upon testing five target viral strains (SARS-CoV-2, FluA, FluB, RSV A, and RSV B), the analytical performance of SC2FabR was similar to that of the other kits, with no significant difference (p ≥ 0.78) in z-scores. The efficiency of SC2FabR (E-value, 81–104%) enabled reliable SARS-CoV-2 and seasonal RV detection in 888 nasopharyngeal swab specimens processed using a fully automated nucleic acid extraction platform. Bland–Altman analyses revealed an agreement value of 95.4% (SD ± 1.96) for the kits, indicating statistically similar results for all five. In conclusion, SC2FabR is a rapid and accurate diagnostic tool for both SARS-CoV-2 and seasonal RV detection, allowing for high-throughput RV analysis with efficiency comparable to that of commercially available kits. This can be used to help manage respiratory infections in patients during and after the coronavirus disease 2019 pandemic.

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Eibinger, Gerald M., Harald H. Kessler, Evelyn Stelzl, Klaus Vander, Anita Weber-Lassacher, Wilfried Renner i Markus Herrmann. "SARS-CoV-2 RNA Testing Using Different Assays—Impact on Testing Strategies in a Clinical Setting". International Journal of Molecular Sciences 23, nr 21 (25.10.2022): 12845. http://dx.doi.org/10.3390/ijms232112845.

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In order to assess SARS-CoV-2 real time quantitative polymerase chain reaction (RT-qPCR) results in a real-life setting, three independent laboratories in Graz (Austria) set up a continuous cross comparison schedule. The following test systems were used: The QIAGEN NeuMoDx SARS-CoV-2 Assay, the Allplex™ 2019-nCoV Assay (Seegene) on a MicroLab Nimbus (Hamilton) platform combined with RealStar SARS-CoV-2 RT-PCR Assay (Altona Diagnostics GmbH), and the cobas SARS-CoV-2 test on a fully automated cobas 6800 system (Roche). A total of 200 samples were analysed, 184 (92%) were found to be concordant with all testing platforms, 14 (7%) discordant. Two (1%) samples tested invalid on a single platform and were excluded from further analysis. Discordant results were distributed randomly across the assays. The Ct values from all assays correlated closely with each other. All discordant samples showed Ct values ≥ 26. SARS-CoV-2 RT-qPCR assays may show considerable variability, especially in samples with low viral RNA concentrations. Decision makers should thus balance the advantages and disadvantages of RT-qPCR for mass screening and adopt suitable strategies that ensure a rational management of positive samples with high Ct values.

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Strojan Fležar, Margareta, Neža Nedelko, Mario Poljak, Anja Oštrbenk Valenčak i Helena Gutnik. "Stratified Mucin-Producing Intraepithelial Lesion (SMILE) of the Uterine Cervix: High-Risk HPV Genotype Predominance and p40 Immunophenotype". Cells 10, nr 8 (10.08.2021): 2039. http://dx.doi.org/10.3390/cells10082039.

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Stratified mucin-producing intraepithelial lesion (SMILE) is a rare high-grade cervical precancerous lesion designated a variant of adenocarcinoma in situ (AIS) in the WHO classification. We aimed to determine HPV genotypes, immunohistochemical phenotype and mucin presence in SMILE. Between 2010 and 2018, SMILE was diagnosed in 34 out of 6958 (0.5%) cervical biopsies, in 23 patients. Twenty-six tissue samples from twenty-one patients were available for further analysis, including 13 with SMILE alone, 12 with SIL and/or AIS and one with HSIL, AIS and endocervical adenocarcinoma. HPV genotyping was performed using the Seegene Anyplex II HPV 28 assay. Of the 26 samples, a single HPV genotype was identified in the majority of cases (n = 22), including 12/13 SMILEs associated with SIL/AIS. All but one were high-risk HPV genotypes (23/24; 96.8%). We identified seven different HPV genotypes, the most common being HPV16 (n = 10; 43.5%), HPV18 (n = 8, 34.8%) and HPV 31 (n = 5, 21.7%). All SMILEs showed a strong positive reaction to p16, CK7, CK19 and high Ki67 expression comparable to adjacent HSIL and/or AIS if present. SMILE showed variable mucin presence and p40-positive squamous differentiation suggesting phenotypic diversity in cervical precancerous lesions infected by single HPV.

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Dossou, Nefert Candace, Isidore Gaubert, Elodie Maille, Remy Morello, Renaud Cassier, Cécile Schanen, Jean-Jacques Dutheil, Louis-Marie Rocque, Astrid Vabret i Meriadeg Ar Gouilh. "Use of LoopDeelab during the COVID-19 Pandemic: An Innovative Device for Field Diagnosis". Viruses 14, nr 9 (17.09.2022): 2062. http://dx.doi.org/10.3390/v14092062.

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Rapid and accurate diagnosis of SARS-CoV-2 infection is essential for the management of the COVID-19 outbreak. RT-LAMP LoopDeetect COVID-19 (LoopDeescience, France) is a rapid molecular diagnostic tool which operates with the LoopDeelab (LoopDeescience, France) device. RAPID COVID is a prospective double-blind research protocol which was conducted to evaluate the concordance between Loopdeetect COVID-19 and RT-PCR Allplex 2019 n-Cov (Seegene, Korea). Between 11 May 2020 and 14 June 2021, a total of 1122 nasopharyngeal swab specimens were collected, of which 741 were finally analysed. There were 32 “positive” and “indeterminate” RT-PCR results. The intrinsic performances of Loopdeetect COVID-19 are equivalent to other commercial RT-LAMP PCR COVID-19 kits, with a sensitivity and specificity of 69.23% [CI 95%: 48.21–85.67] and 100% [CI 95%: 99.58–100.00], respectively. To the best of our knowledge, LoopDeelab is the only LAMP PCR diagnostic device allowing such a fast and reliable analysis with low-cost equipment; this makes it a new and innovative technology, designed for field use. This device being portable, the development of other detection kits will be useful for the management of epidemics with a high attack rate and would facilitate the rapid application of health measures.

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Fattouh, Ramzi, Karel Boissinot, Esther Jeong, Andrew B. Mendlowitz, Calvin P. Sjaarda, Henry Wong, Robert Kozak, Prameet M. Sheth i Larissa M. Matukas. "Evaluation of 5 Polymerase Chain Reaction Assays for the Detection of Mpox Virus". Journal of Infectious Diseases 229, Supplement_2 (26.03.2024): S156—S162. http://dx.doi.org/10.1093/infdis/jiad464.

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Abstract Background In 2022, the global dissemination of mpox virus (MPXV) outside endemic regions prompted the expansion of diagnostic testing worldwide. This study assesses the performance characteristics of 5 real-time polymerase chain reaction (PCR) assays in detecting MPXV during the 2022 outbreak. Methods Clinical specimens collected from patients across Ontario, Canada, were tested on the following assays: RealStar Orthopoxyvirus PCR and FlexStar Monkeypox virus PCR (Altona Diagnostics), Novaplex MPXV (Seegene), VIASURE Monkeypox virus Real Time PCR Reagents (CerTest Biotec), and a laboratory-developed test. Positive percent agreement (PPA), negative percent agreement (NPA), relative limit of detection (LOD), and precision were evaluated and MPXV lineages were determined using an amplicon-based whole-genome sequencing (WGS) assay. Results Swabs were collected from various anatomic sites (65 positive and 30 negative). All assays demonstrated 100% NPA (95% confidence interval, 88.4%/88.1%–100.0%), with PPA ranging from 92.2% (82.7%–97.4%) to 96.9% (89.3%–99.6%). LOD and precision were comparable across assays, with coefficient of variations <3%. WGS analysis identified 6 lineages, all belonging to subclade IIb. Conclusions The assays exhibited excellent PPA, NPA, LOD, and precision. Ongoing performance monitoring is essential to detect assay escape mutants and ensure universal detection of evolving MPXV strains.

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Specchiarello, Eliana, Giulia Matusali, Fabrizio Carletti, Cesare Ernesto Maria Gruber, Lavinia Fabeni, Claudia Minosse, Emanuela Giombini i in. "Detection of SARS-CoV-2 Variants via Different Diagnostics Assays Based on Single-Nucleotide Polymorphism Analysis". Diagnostics 13, nr 9 (27.04.2023): 1573. http://dx.doi.org/10.3390/diagnostics13091573.

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Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is characterized by fast evolution with the appearance of several variants. Next-Generation Sequencing (NGS) technology is considered the gold standard for monitoring known and new SARS-CoV-2 variants. However, the complexity of this technology renders this approach impracticable in laboratories located in areas with limited resources. We analyzed the capability of the ThermoFisher TaqPath COVID-19 RT-PCR (TaqPath) and the Seegene Novaplex SARS-CoV-2 Variant assay (Novaplex) to detect Omicron variants; the Allplex VariantII (Allplex) was also evaluated for Delta variants. Sanger sequencing (SaS) was the reference method. The results obtained with n = 355 nasopharyngeal samples were: negative with TaqPath, although positive with other qualitative molecular assays (n = 35); undetermined (n = 40) with both the assays; negative for the ∆69/70 mutation and confirmed as the Delta variant via SaS (n = 100); positive for ∆69/70 and confirmed as Omicron BA.1 via SaS (n = 80); negative for ∆69/70 and typed as Omicron BA.2 via SaS (n = 80). Novaplex typed 27.5% of samples as undetermined with TaqPath, 11.4% of samples as negative with TaqPath, and confirmed 100% of samples were Omicron subtypes. In total, 99/100 samples were confirmed as the Delta variant with Allplex with a positive per cent agreement (PPA) of 98% compared to SaS. As undermined samples with Novaplex showed RdRp median Ct values (Ct = 35.4) statistically higher than those of typed samples (median Ct value = 22.0; p < 0.0001, Mann–Whitney test), the inability to establish SARS-CoV-2 variants was probably linked to the low viral load. No amplification was obtained with SaS among all 35 negative TaqPath samples. Overall, 20% of samples which were typed as negative or undetermined with TaqPath, and among them, twelve were not typed even by SaS, but they were instead correctly identified with Novaplex. Although full-genome sequencing remains the elected method to characterize new strains, our data show the high ability of a SNP-based assay to identify VOCs, also resolving samples typed as undetermined with TaqPath.

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Hint, Helen, Piia Taremaa, Maria Reile i Renate Pajusalu. "Demonstratiivpronoomenid ja -adverbid määratlejatena. Miks me oleme siin ilmas, selles olukorras?" Eesti ja soome-ugri keeleteaduse ajakiri. Journal of Estonian and Finno-Ugric Linguistics 12, nr 1 (6.09.2021): 79–111. http://dx.doi.org/10.12697/jeful.2021.12.1.03.

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Kokkuvõte. Artiklis analüüsime eesti keele demonstratiivide referentsiaalseid omadusi sellistes konstruktsioonides, kus demonstratiivid kuuluvad definiitse määratlejana nimisõnafraasi koosseisu. Otsime vastust küsimusele, mille poolest erinevad demonstratiivadverb (nt siin, seal) ning demonstratiivpronoomen (see, too), kui need esinevad määratlejana koos kohakäändes nimisõnafraasiga (vrd siin koolis ja selles koolis). Oleme püstitanud hüpoteesi, et demonstratiivadverbid seostuvad ruumitähendust väljendavate substantiividega, demonstratiivpronoomenid esinevad aga nende substantiividega, mille referent on mitteruumiline. Uurimuse andmestik pärineb 2017. aasta eesti keele ühendkorpusest, kust oleme võtnud 100 lauset iga demonstratiivi kohta igas kohakäändes, seega kokku 2400 lauset. Materjali analüüsime kvantitatiivselt (tingimuslike otsustuspuude ja juhumetsadega) ning kvalitatiivselt. Uurimuse tulemused kinnitavad, et substantiivi semantilised omadused, täpsemalt substantiivi semantiline klass ning konkreetsus, on seotud määratleja valikuga. Kohatähenduses substantiividega esineb määratlejana sagedamini demonstratiivadverb, mittekoha tähenduses substantiivide määratlejana kasutatakse aga demonstratiivpronoomenit. Mittekohta tähistavate substantiivide korral mõjutab määratleja valikut omakorda sõna konkreetsus. Seega on võimalik demonstratiivseid määratlejaid eesti keeles kasutada referenti looval viisil. Abstract. Helen Hint, Piia Taremaa, Maria Reile, Renate Pajusalu: Demonstrative pronouns and demonstrative adverbs as determiners in Estonian: why are we in “here world” in “this situation”? We investigate the variation of definite determiner constructions in Estonian: noun phrases with a demonstrative pronoun (see ‘this’, too ‘that’) or demonstrative adverb (siin ‘here’, seal ‘there’) as a determiner are contrasted. The question is what differentiates the use of a demonstrative pronoun and a demonstrative adverb if used in a determiner position in an NP. The data from Estonian National Corpus 2017 were tagged for semantic class of a noun, noun concreteness, and verb type. We collected 100 clauses for each sub-construction (six spatial cases crossed with four determiner forms), 2400 clauses in total. For statistical analysis, we used conditional random forests and inference trees. We show that nouns expressing spatial meaning prefer demonstrative adverbs as determiners, while non-spatial nouns combine with demonstrative pronouns. Spatiality-wise polysemous nouns exhibit more varied preferences. Adverbial determiners are more probably used with concrete nouns, and abstract nouns co-occur with pronominals. Overall, the frequency of demonstrative adverbs as NP attributes confirms that demonstrative adverbs are productive determiners in Estonian.

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Alkhatib, Mohammad, Maria Concetta Bellocchi, Greta Marchegiani, Sandro Grelli, Valeria Micheli, Daniele Stella, Bartolomeo Zerillo i in. "First Case of a COVID-19 Patient Infected by Delta AY.4 with a Rare Deletion Leading to a N Gene Target Failure by a Specific Real Time PCR Assay: Novel Omicron VOC Might Be Doing Similar Scenario?" Microorganisms 10, nr 2 (25.01.2022): 268. http://dx.doi.org/10.3390/microorganisms10020268.

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Herein, we report a case of an Italian male infected by Delta sublineage AY.4 harboring an atypical deletion, leading to a N gene target failure (NGTF) by a commercial molecular assay for SARS-CoV-2 diagnosis (AllplexTM SARS-CoV-2 Assay, Seegene). A 59-year-old unvaccinated patient was hospitalized for pulmonary embolism, with first negative results obtained by both molecular and antigen tests. After several days of viral negativity, he presented positive results for E and RdRP/S genes, but negative in N gene. Negativity in N gene was repeatedly confirmed in the following days. Suspecting an infection by the Omicron variant, SARS-CoV-2 genome sequencing was rapidly performed from nasopharyngeal swab by MiSeq and revealed the presence of the Delta sublineage AY.4 variant with an atypical deletion of six nucleotides, leading to G214-G215 deletion in the Nucleocapsid, thus responsible for NGTF. The analysis of GISAID sequences (N = 2,618,373 12 January 2022) showed that G214-G215 deletion is rarely occurring in most circulating Delta lineages and sublineages in the globe and Europe, with an overall prevalence never exceeding 0.2%. Hence, this study highlights the importance to perform SARS-CoV-2 sequencing and to characterize novel mutations/deletions that could jeopardize the proper interpretation of molecular diagnostic tests. Based on these assumptions, the role of deletions in the recently identified Omicron variant deserves further investigation.

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Rozprawy doktorskie na temat "Seegage analysis"

Hennig, Janou. "Generation and analysis of harsh wave environments". [S.l.] : [s.n.], 2005. http://deposit.ddb.de/cgi-bin/dokserv?idn=975328727.

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Gaslikova, Lidia. "High-resolution wave climate analysis in the Helgoland area /". Geesthacht : GKSS-Forschungszentrum, Library, 2006. http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&doc_number=015585234&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA.

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