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Overton, Julia Lynne. "Morphometric, genetic and reproductive characteristics of mud crabs (genus Scylla de Haan, 1833) from Southeast Asia". Thesis, University of Stirling, 2000. http://hdl.handle.net/1893/2599.
Pełny tekst źródłaTruong, Phuong Ha. "Nutrition and feeding behaviour in two species of mud crabs Scylla serrata and Scylla paramamosain". Thesis, Queensland University of Technology, 2008. https://eprints.qut.edu.au/20700/1/Phuong_Truong_Thesis.pdf.
Pełny tekst źródłaTruong, Phuong Ha. "Nutrition and feeding behaviour in two species of mud crabs Scylla serrata and Scylla paramamosain". Queensland University of Technology, 2008. http://eprints.qut.edu.au/20700/.
Pełny tekst źródłaDelathière, Stephen. "Biologie et exploitation du crabe de palétuviers Scylla serrata en Nouvelle Calédonie". Brest, 1990. http://www.theses.fr/1990BRES2015.
Pełny tekst źródłaPavasovic, Marko. "Digestive profile and capacity of the mud crab (Scylla serrata)". Thesis, Queensland University of Technology, 2004. https://eprints.qut.edu.au/15973/1/Marko_Pavasovic_Thesis.pdf.
Pełny tekst źródłaPavasovic, Marko. "Digestive profile and capacity of the mud crab (Scylla serrata)". Queensland University of Technology, 2004. http://eprints.qut.edu.au/15973/.
Pełny tekst źródłaChurchill, Giles John. "An investigation into the captive spawning, egg characteristics and egg quality of the mud crab (Scylla serrata) in South Africa". Thesis, Rhodes University, 2004. http://hdl.handle.net/10962/d1015963.
Pełny tekst źródłaGopurenko, David, i n/a. "Genetic Structure Within the Distribution of the Indo-West Pacific Mud Crab Scylla serrata (Forskal, 1775)". Griffith University. Australian School of Environmental Studies, 2003. http://www4.gu.edu.au:8080/adt-root/public/adt-QGU20030926.124631.
Pełny tekst źródłaGopurenko, David. "Genetic Structure Within the Distribution of the Indo-West Pacific Mud Crab Scylla serrata (Forskal, 1775)". Thesis, Griffith University, 2003. http://hdl.handle.net/10072/367817.
Pełny tekst źródłaThesis (PhD Doctorate)
Doctor of Philosophy (PhD)
Australian School of Environmental Studies
Full Text
Webley, James A. C. "The ecology of the mud crab (Scylla serrata): their colonisation of estuaries and role as scavengers in ecosystem processes". Thesis, Griffith University, 2008. http://hdl.handle.net/10072/367091.
Pełny tekst źródłaThesis (PhD Doctorate)
Doctor of Philosophy (PhD)
School of Environment
Science, Environment, Engineering and Technology
Full Text
Mirera, David Oersted. "Capture-based mud crab (Scylla serrata) aquaculture and artisanal fishery in East Africa- Practical and ecological perspectives : Mud crab ecology and aquaculture". Doctoral thesis, Linnéuniversitetet, Institutionen för biologi och miljö (BOM), 2014. http://urn.kb.se/resolve?urn=urn:nbn:se:lnu:diva-32399.
Pełny tekst źródłaNguyen, Thi Bich Ngoc. "Commercial mud crab Scylla Serrata : Study on growth, energy and protein requirement of juveniles in the view to develop peleted feed for crab farming in New Caledonia". Thesis, Nouvelle Calédonie, 2014. http://www.theses.fr/2014NCAL0057/document.
Pełny tekst źródłaIn New Caledonia, there is the strong political will to diversify aquaculture which is mainly based on shrimp farming. In this context, mud crabs have been considered as a potential species for aquaculture development. One of the main constraints to develop crab farming is the availability of formulated feed. Thus, the main purpose of this thesis is to get information on the crab nutritional requirements in order to formulate a balanced diet. However, we had to clarify first how many species of mud crab were present in New-Caledonia. The result of our morphological and genetic investigations carried out on 63 specimens from 9 areas of the west and northeast coast of New-Caledonia confirmed that only one species, Scylla serrata, is commercialized in this country. Consequently, S. serrata was used in our nutritional study based on two experiments to: i) evaluate the soy protein concentrate (SPC) compared with the fishmeal as the main protein source and ii) determine the optimum level of SPC in the diet for molting and tissue growth. We observed two tissue growth phases within one molt cycle (MC): a fast tissue growth (FTG) occurred after ecdysis until early intermolt stage (30% of MC) which is followed by a slow tissue growth (STG) period from intermolt to ecdysis (70% of MC). Protein and lipid deposition followed the same trend than tissue growth while ash level increased quickly during five days after molt and then remained stable until the next molt. The two growth phases were correlated with the voluntary feed intakes (VFI) which was maximum during 2 weeks after ecdysis and then decreased by 50% over the five following weeks to reach a baseline until the next molt. Intake energy was allocated mainly for growth during FTG period and for maintenance during STG period. During STG, 28% of the ingested energy was accumulated for the next ecdysis. Replacement of fishmeal by SPC as main protein source did not affect tissue growth, efficiency of feed utilization and energy budget of crabs whatever the tissue growth period considered. The dietary SPC inclusion of 42% in the diet promoted growth (molt frequency and tissue growth), feed efficiency and retention of energy, protein and lipid. Hypothesis related to ammonia toxicity from catabolism of proteins in excess or anti-nutritional factors from soybean could explain the negative effects of higher inclusion of SPC in the diet for juvenile crabs. In conclusion, our work brings novel information on tissue growth, energy budget during a molt cycle and the ability of juvenile crab to use SPC as a main source of protein. On this basis we suggest to formulate nutritionally balanced diet without fishmeal to farm juvenile mud crabs S. serrata
Alberts-Hubatsch, Hilke [Verfasser], Matthias [Akademischer Betreuer] Wolff i Shing Yip [Akademischer Betreuer] Lee. "Movement patterns and habitat use of the exploited swimming crab Scylla serrata (Forskål, 1775) / Hilke Alberts-Hubatsch. Gutachter: Matthias Wolff ; Shing Yip Lee. Betreuer: Matthias Wolff". Bremen : Staats- und Universitätsbibliothek Bremen, 2015. http://d-nb.info/1072303779/34.
Pełny tekst źródłaZhang, Zhong Zuo, i 張仲佐. "Studies on protein and energy for juvenile mud crab, scylla serrata". Thesis, 1994. http://ndltd.ncl.edu.tw/handle/13934960557113483504.
Pełny tekst źródła陳文彬. "Effect of Salinity Acclimation on Chemical Constituents of mud crab (Scylla serrata)". Thesis, 2002. http://ndltd.ncl.edu.tw/handle/85739719645374943956.
Pełny tekst źródła國立海洋大學
食品科學系
90
Abstract The purpose of this study was to investigate the changes of chemical constituents in the abdominal muscle of female mud crab in relation to short-term salinity acclimation and subsequent air-exposured storage at 15℃. The crab specimens were acclimated to 25 ppt of sea water for 24 hours (control) and then transferred to 10 and 40 ppt sea water for a period of 4~48 hours. In comparison with control, moisture in 40 ppt sample decreased, but that in 10 ppt sample increased. On contrary, the glycogen showed a inverse trend. Taurine (Tau), glutamine (Gln), proline (Pro), glycine (Gly), alanine (Ala) and arginine (Arg) were dominant free amino acids (FAA) and they together accounted for about 87﹪of the total FAA. Adenosine triphosphate (ATP) and glycinebetaine (GB) were respectively the major component among the ATP-related compounds (ARC) and quternary ammonium bases (QAB) detected. As a whole, the 40 ppt sample was relatively high in levels of the total FAA, total ARC, GB, and homarine (Hom). In addition, the difference in levels of moisture, glycogen, total FAA, total ARC, GB, and Hom between 10 and 40 ppt samples increased with the elongation of acclimation time. The crab specimens acclimated to 10 ppt, 25 ppt, and 40 ppt sea water and then stored at 15℃ for 12~72 hours. Differences in the moisture and glycogen levels declined after storage. The total FAA in all samples elevated gradually with the storage time and the sample acclimated to higher salinity was more pronounced. The major FAA except for Tau also increased. After storage, the total ARC content was higher in the 40 ppt sample than in other two samples. The ATP decreased with storage time, while its breakdown products accumulated. The GB and Hom contents were higher in the sample acclimated to higher salinity, but their difference between the samples lowered as the storage time increased. Of the samples acclimated to 10、25, and 40 ppt sea water for 48 hours, their hot-water extracts were prepared and compared by sensory test for taste. Taste intensity of the extracts was higher in the 25 and 40 ppt samples than in the 10 ppt sample. The salt, Na+, and total FAA contents in the hot-water extracts followed the order of 40 ppt > 25 ppt > 10 ppt sample. Levels of glycogen, GB, and Hom were higher in the 25 ppt sample than in the 40 ppt and 10 ppt samples. However, there was no significant difference in the total amounts of ARC between them.
(9777185), Leonie Andersen. "A study into the epidemiology of mud crab (Scylla serrata) shell disease". Thesis, 2003. https://figshare.com/articles/thesis/A_study_into_the_epidemiology_of_mud_crab_Scylla_serrata_shell_disease/13417376.
Pełny tekst źródła黃瑞蓬. "Studies on the chemical constituents and flavor of cultured mangrove crab(Scylla serrata)". Thesis, 2000. http://ndltd.ncl.edu.tw/handle/68642068636467257290.
Pełny tekst źródłaWu, Sheng Wei, i 吳聖偉. "Evaluation of dietary oil、fatty acid and cholesterol requirements of postlarval scylla serrata". Thesis, 1994. http://ndltd.ncl.edu.tw/handle/90681661241155547339.
Pełny tekst źródłaCHIANG, MING-TA, i 蔣明達. "The heart beats and behavioral response of Scylla Serrata to different frequency sounds". Thesis, 2019. http://ndltd.ncl.edu.tw/handle/9m277h.
Pełny tekst źródła國立宜蘭大學
生物機電工程學系碩士班
107
Anthropogenic noise has been recognized as a major source of environmental pollution, including frequent shipping, sonar detection, generator sets and river engineering, which have been shown to have a variety of negative impacts on organisms. This study uses sinusoidal waves to stimulate Scylla serrata, and records behavior and electrocardiogram to explore whether sinusoidal noise has a specific frequency band that can significantly affect the behavior and heart rate of Scylla serrata. The experiment was divided into two batches: the batch of experiments (O1~O6) which noise stimulations were shifted from low to high in the order of eight frequencies of 100 Hz, 400 Hz, 700 Hz, 1000 Hz, 1300 Hz, 1600 Hz, 1900 Hz, and 2200 Hz. The second batch of experiments (R1~R6) which noise stimulations were randomed from the 8 frequencies of the first batch of experiments. The experimental results showed that the behavior, under the 100 Hz, the proportion of crab moving after sound playback is greater than other higher frequencies. Although there is no statistically significant difference, in most of the stimulation frequencies, the behavior of the first stimulation is more intense. The heart rate after stimulation is decreased, which can be inferred. When the environmental sound changes, the internal physiology also changes synchronously. It was also found in the experiment that the rate of decline in heart rate was higher than that of the second and third stimulations during the first stimulation, which may be due to the adaptation of crab to sound.
Chung, Kuei-Fang, i 鍾桂芳. "Na+, K+-ATPase Activity and Protein Level in the Osmoregulatory Organs of Scylla serrata". Thesis, 2002. http://ndltd.ncl.edu.tw/handle/05548184945686890779.
Pełny tekst źródła東海大學
生物學系
90
Scylla serrata is a euryhaline marine crab, which can tolerate a wide range of salinities between 1 ppt and 42 ppt. And the salinity fluctuation may cause serious changes in hemolymph osmolality and influence the physiological functions. The osmoregulatory organs of crab primarily include gills, antennal glands and guts. Most studies on crustacean osmoregulation had their focus on the gills only, but very rarely were these organs investigated simultaneously. Since Na+, K+-ATPase has been known as the driving force of ions transportations, it can be taken as a valid indicator of osmoregulatory ability. From previous literatures, posterior gills, antennal gland and gut respectively showed a higher Na+, K+-ATPase activity when the crabs were kept in diluted seawater. This implies that the relative importance in osmoregulation among these organs is not identical. And whether this increase resulted from the modulation of pre-existing Na+, K+-ATPase or new protein synthesis is still unclear. In this study, after transferring S. serrata from 25 ppt to 5, 25 and 45 ppt respectively, hemolymph osmolality, Na+, K+-ATPase activity and the level of Na+, K+-ATPase α-subunit in western blotting were examined at different time intervals. The results indicated that S. serrata maintained their hemolymph osmolality significantly higher than their respective environments of 5 and 25 ppt, therefore, it should be categorized as a hyper-osmoregulator. The posterior gill (gill 6) and antennal gland were significantly higher than the anterior gill (gill 2) and gut in both the Na+, K+-ATPase activities and their α-subunit levels. Upon 14 days after transfer, the activity of the posterior gill was significantly higher in 5 ppt than in the other two salinity treatments. Such differences among salinity levels were not observed in other organs. In the results of western blotting, salinity did not cause a significant change in the protein levels of Na+, K+-ATPase α-subunit among these osmoregulatory organs, except the gut in the first day after transferring. The protein level of Na+, K+-ATPase α-subunit did not change significantly in the posterior gill while the enzyme activity fluctuated with environmental salinity. In conclusion, the posterior gills played the most important role in osmoregulation when the crabs were in diluted SW and the activity of the enzyme might be modulated by the pre-existing Na+, K+-ATPase.
Liessmann, Laurence. "Investigation into the mortalities of larval mud crabs, Scylla serrata and methods of control". Thesis, 2005. https://researchonline.jcu.edu.au/17553/1/01front.pdf.
Pełny tekst źródłaTsou, Li-Tse, i 鄒禮澤. "The classification and non-specific immune functions of the haemocytes of the mud crab (Scylla serrata)". Thesis, 2007. http://ndltd.ncl.edu.tw/handle/70550779573927340900.
Pełny tekst źródła國立中興大學
獸醫學系暨研究所
95
The aim of this study was to investigate the morphology and haemocytic immune functions of haemocytes in mud crab (Scylla serrata). The effects of immunostimulants on mud crab haemocytes were also studied in vitro. Our results revealed that flow cytometry could successfully classify the crab’s haemocytes into three populations: hyalinocytes, semi-granulocytes and granulocytes. For young female crabs, the percentages of hyalinocytes, semi-granulocytes and granulocytes were 33.70%, 43.47%, and 5.06%, respectively. Because the percoll gradient centrifugation method could not clearly distinguish each subpopulation, we applied flow cytometry to differentiate among the different groups of haemocytes. Our data showed that our technique was capable of separating the haemocytes. The purities of hyalinocytes, semi-granulocytes and granulocytes were 83.1%, 86.8% and 76.9%, respectively. The morphology of the three haemocytes were observed by optical microscopy, fluorescence microscopy, and TEM, respectively. In this study, eight kinds of immunostimulants, including five types of glucan (laminarin, levan, scleroglucan, yeast glucan, and zymosan) and three different sources of lipopolysaccharide (LPS) from Escherichia coli, Pseudomonas aeruginosa and Salmonella enterica were used. Our data suggested that the working dosage of yeast glucan, zymosan, LPS E and LPS P required to induce the crab’s innate immune responses was 20 μg/mL. Interestingly, we found that immunostimulants could change the proportions of the crab’s haemocytic subpopulations, especially for hyalinocytes which increased markedly. The percentages of semi-granulocytes and granulocytes, however, decreased. In order to investigate these changes, a cell proliferation assay was conducted. There were no apparent increases in haemocytic subpopulations when cultured with glucan or LPS. Our data suggested that the cause of changes in haemocytic proportion was subpopulation shift by degranulation, rather than by cell proliferation. Additionally, we confirmed that prophenoloxidase (proPO) was located in haemocytes but not in serum. Moreover, proPO activity could be induced by SDS, trypsin, zymosan, and LPS E. However, it was inhibited by soybean trypsin inhibiter. According to respiratory burst reaction and phagocytosis assay, our results indicated that granulocytes had the strongest phagocytosis and respiratory burst abilities among the three haemocytes. Hyalinocytes had very low phagocytosis and respiratory burst abilities. The abilities of semi-granulocytes were lower than those of granulocytes but higher than those of hyalinocytes. Our results showed that the innate immune response of haemocytes was capable of being enhanced by exposure to high concentrations of bacteria. We found their survival rate as well as respiratory burst and phagocytosis abilities were significantly increased after the cells were treated with immunosimulants (P<0.05).
Hung, Chia-Wei, i 洪嘉偉. "Studies on the Pathogenesis and Pathology of Scylla serrata infected by Vibrio alginolyticus and Vibrio parahaemolyticus". Thesis, 2010. http://ndltd.ncl.edu.tw/handle/49671102551515495944.
Pełny tekst źródła國立臺灣海洋大學
水產養殖學系
98
This thesis investigated the pathogenicity of Vibrio on mud crab (Scylla serrata) cultured in Taiwan together with and pathological of seuration. Scylla serrata was susceptible to Vibriosis leading to death with the symptoms including yellowing of chelating feet, whitish and erosion of muscle, abnormal in the colour (often yellowish brown) and coagulation hemolymph, erosion in hepatopancreas and so on. Bacteria were isolated from diseased Scylla serrata, with Vibrio parahaemolyticus and V. alginolyticus as the two dominant pathogenic species. In this study, virulent Vibrio parahaemolyticus (HP1) and V. alginolyticus (YH5) were selected for virulence challenge tests. The LD50 values of V. parahaemolyticus (HP1) bacteria and its extracellular products (ECP) were 2.24 × 105 CFU/g and 0.15 μg protein/g crab, respectively; and for V. alginolyticus (YH5), the LD50 values of values of bacteria and its ECP were 3.16 × 104 CFU/g and 0.42 μg protein/g crab, respectively. In the virulence tests, both of the two Vibrio species caused rapid death in the mud crab challenged with bacteria and ECP. The average time of each individual mud crab died caused by of V. alginolyticus (YH5) and its ECP were 180 minutes and 60 mins, respectively, while V. parahaemolyticus (HP1) were 140 mins and 50 mins, respectively. Similar symptoms on the mud crabs could be observed with eyestalk retraction in the beginning, followed by no response to external stimuli and individual paralysis, and some individuals with self amputation (autotomy). Erosion of the hepatopancreas was observed in most of the moribund and freshly died mud crab with no other obvious symptom. Tissues of moribund crab after challenge with bacteria and ECP were fixed with Davidson’s solution for further histopathological V observation. Mud crab challenged with V. alginolyticus(YH5) and V. parahaemolyticus(HP1) exhibited muscle fibres necrosis and atrophy, the presence of bacteria cluster in some tissues, and decrease of blood lymphocytes in necrotic tissues. The pathological difference between the two of bacterial strains was that abnormal material deposition visualized as bright pink could be observed only in muscle challenged with strain HP1. In the hepatopancreas, cell degeneration and necrosis of microtubules were observed, together with disappearance on blur of cell contours and the deposition of red light bacterial cells with different sizes. Necrosis of muscle fibres could be observed in crab treated with ECP of both two Vibrio strains. Visible necrosis and vacuolization of microtubules and together with loss of epithelial cells of luman, and bright pink abnormal material deposition could only be visualized in samples treated with strain HP1. This study showed that V. parahaemolyticus and V. alginolyticus were important pathogens of Scylla serrata, which could cause infection during the culture of Scylla serrata. Further studies are needed to understand the pathogenesis of these two Vibrio species in mud crab.
Holme, May-Helen. "Towards development of a formulated diet for mud crab (Scylla serrata) larvae, with emphasis on lipid nutrition". Thesis, 2008. https://researchonline.jcu.edu.au/2150/1/01front.pdf.
Pełny tekst źródłaLin, Yong-Chin, i 林永慶. "Cloning, characterization and expression of α2-macroglobulin and its evolution in Penaeus monodon、Litopenaeus vannamei and Scylla serrata". Thesis, 2008. http://ndltd.ncl.edu.tw/handle/04059972653606259012.
Pełny tekst źródła何惠玟. "The Qualotative and Quantitative Effect of Dietary Phosphatidylholine on Growth and Tissue Fatty Acids of Juvenile Scylla Serrata". Thesis, 1998. http://ndltd.ncl.edu.tw/handle/42216199219016989082.
Pełny tekst źródła國立海洋大學
水產養殖學系
86
This study investigated the qualitative and quantitative effects of dietary phosphatidylcholine on the growth and tissue fatty acids of juvenile Scylla serrata. In experiment 1,4% cod liver oil was used as lipid source. Diets containing 0, 1, 2, 3, and 4% chicken phosphatidylcholine, and 2.4% choline chloride were evaluated. Crabs fed diets containing 1, 2, 3, and 4% phosphatidylcholine had significantly higher weight gain than those fed diet without supplemented phosphatidylcholine. Crabs fed diet supplemented with 2.4% choline chloride had lower weight gain than those fed diets containing 2, 3, 4% phosphatidylcholine. The survival rate ranged from 80% to 100%. Crabs mean molting frequency were not increased when they were fed diets containing phosphatidylcholine. Crabs fed diets containing phosphatidylcholine had higher polar lipid fraction of n-3 HUFA retention. The effect of different phosphatidylcholine sources on the growth rate of S. serrata were investigated in experiment II. The treatment diets containing 3% cod liver oil were included with 1% of SPC (soybean phosphatidylcholine, 20% purity), 1% of SPC(soybean phosphatidylcholine, 40% purity), 1% of FPC(fresh egg phosphatidylcholine, 60% purity), 1% of FSL(fluid soybean lecithin, 15% purity), 1% of DSL (deoil soybean lecithin, 23% purity). The diet including 4% cod liver oil was used as control diet. Crabs fed diet containing FSL had the highest weight gain than those fed other treatments. Crabs fed diets supplemented with different kinds of phosphatidylcholine had higher lever of cholesterol and phosphatidylcholine than that those fed diet supplemented without phosphatidylcholine. The survival rate ranged from 80% to 100%.
Xie, Ping Yu, i 謝平玉. "Effects of different salinity and temperature levels on nitrogen metabolism and ions regulation of mud crab scylla serrata". Thesis, 1994. http://ndltd.ncl.edu.tw/handle/22767177718392243400.
Pełny tekst źródłaGenodepa, Jerome G. "Digestive enzyme dynamics during early life stages of the mud crab, Scylla serrata and the spiny lobster, Panulirus ornatus". Thesis, 2015. https://researchonline.jcu.edu.au/46242/1/46242-genodepa-2015-thesis.pdf.
Pełny tekst źródłaLin, Jong Huzy, i 林仲惠. "Studies on the taxonomic status and population genetics of the mud crab Scylla serrata (Crustacea:Decapoda:Brachyura) by using morphometric,biochemical and behavioral methods". Thesis, 1993. http://ndltd.ncl.edu.tw/handle/78828930849703898201.
Pełny tekst źródłaLin, Yea-Jen, i 林雅真. "Responses of juvenile mud crab (Scylla serrata) to different oil sources and different levels of n-3 or n-6 polyunsaturated fatty acids". Thesis, 1996. http://ndltd.ncl.edu.tw/handle/34277339521722223425.
Pełny tekst źródła國立海洋大學
水產養殖學系
84
This study was conducted to investigate the effects of diets with different sources of lipid and different quantity of polyunsaturated fatty acids (PUFA) or highly unsaturated fatty acids (HUFA) on the growth and survival of juvenile crabs. In Experiment 1, juvenile crabs were fed with diets supplemented with 4 % cod liver oil, corn oil, olive oil, soybean oil and sesame oil for 10 weeks. The results showed that juvenile crabs fed with 4 % cod liver oil had the best growth. Juvenile crabs fed with diets containing corn oil, olive oil, soybean oil and sesame oil showed no significant differences. Juvenile crabs fed diets containing cod liver oil had high level of EPA and DHA of body tissue of juvenile crabs. These results showed that the fatty acid composition of tissue of crabs was obviously reflected from fatty acid composition of diet. Weight gain and survival responses and analyses of fatty acid composition of tissue were used to investigate the nutritional value of C18 polyunsaturated fatty acids (PUFA) provided to juvenile crabs in a purified diet. There were seven isonitrogenous and isoenergetic diets containing pure triglycerides of 0.2, 0.4 and 0.6﹪18:3n-3 (trilinolenin) and 18:2n-6 (trilinolein) and 0.2 ﹪22:6n-3 (tridocosahexaenoin) as control diet for experiment 2 . All diets were formulated to contain a total of 4﹪lipid and were fed to juvenile crabs for 12 weeks. The results showed that juvenile crabs fed diets containing 0.2﹪tridocosahexaenoin showed better growth than those fed diets containing trilinolenin and trilinolein. Diets containing high proportion of trilinolein or trilinolenin could not improve the growth of juvenile crabs. The high level of 18:2n-6 and 18:3n-3 in the polar and neutral lipids of tissue of juvenile crabs was found that crabs were fed diets containing trilinolein or trilinolenin. Crab fed diet containing 0.2 % tridocosahexaenoin had high level of EPA and DHA in the polar lipid of crab tissue. Weight gain and survival responses and analyses of fatty acid composition of tissue were used to investigate the nutritional value of C≧20 highly unsaturated fatty acids (HUFA) provided to juvenile crabs in a purified diet. There were isonitrogenous and isoenergetic diets containing pure triglycerides of 0.2, 0.4 and 0.6﹪20:4n-6 (triarachidonin) and 22:6n-3 (tridocosahexaenoin). Reference diet was included cod liver oil as the lipid source. All diets were formulated to contain a total of 4﹪lipid. A diets containing only saturated (14:0, 16:0, and 18:0) and monounsaturated (16:1n-7 and 18:1n-9) fatty acids as lipid sources, in proportions similar to that found in a 2:1 cod liver oil/corn oil mixture, served as a conditioning and control diets for experiment {= 3 \* ROMAN|III}. At the termination of the feeding trials, that juvenile crabs fed diets containing 0.4 % tridocosahexaenoin and 4 % cod liver oil had the best growth. The control group (diet included without PUFA and HUFA) had the lowest growth and survival. Crab fed diets containing 0.6 % triarachidonin and control group had high mortality. Crab fed diets containing triarachidonin (20:4n-6) and tridocosahexaenoin (22:6n-3) had high level of 20:4n-6 and 22:6n-3 in the tissue. However, crab fed diets containing tridocosahexaenoin (22:6n-3) had high level of EPA and DHA in the polar lipid of crab tissue.