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Purcell, Peter James, Tommy M. Boland, Martin O'Brien i Pádraig O'Kiely. "In vitro rumen methane output of forb species sampled in spring and summer". Agricultural and Food Science 21, nr 2 (5.06.2012): 83–90. http://dx.doi.org/10.23986/afsci.4811.
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The chemical composition, in vitro rumen fermentation variables and methane (CH4) output of a range of common forb species sampled in spring and summer, and grass silage (14 treatments in total), were determined in this study. Dried, milled herbage samples were incubated in an in vitro rumen batch culture with rumen microbial inoculum (rumen fluid) and buffered mineral solution (artificial saliva) at 39 °C for 24 hours. All herbage chemical composition and in vitro rumen fermentation variables were affected (p<0.001) by treatment. Rumex obtusifolius (in spring and summer), Urtica dioica (summer) and Senecio jacobaea (summer) had lower (p<0.05) CH4 outputs relative to feed dry matter incubated compared with grass silage, reflecting their lower extent of in vitro rumen fermentation.
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NAGARAJA, T. G., S. J. GALITZER, D. L. HARMON i S. M. DENNIS. "EFFECT OF LASALOCID, MONENSIN AND THIOPEPTIN ON LACTATE PRODUCTION FROM IN VITRO RUMEN FERMENTATION OF STARCH". Canadian Journal of Animal Science 66, nr 1 (1.03.1986): 129–39. http://dx.doi.org/10.4141/cjas86-014.
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Starch fermentations with strained rumen fluid and centrifuged rumen fluid devoid of protozoa were set up to test the effect of lasalocid, monensin, and thiopeptin on L(+) and D(−) lactate production. Protozoa-free rumen fluid was the supernatant from low-speed centrifugation of strained rumen fluid. Starch fermentation in the control (no antibiotic) with centrifuged rumen fluid resulted in higher lactate concentration than the fermentation with strained rumen fluid. Decreased lactate production with strained rumen fluid was attributed to sequestration of starch by protozoa and to enhanced lactate fermentation. Addition of lasalocid or monensin (1.5–48.0 μg mL−1) to the fermentation enhanced L(+) and D(−) lactate production in the presence of protozoa. In the absence of protozoa, lasalocid and monensin inhibited L(+) lactate production; however, D(−) lactate concentration was unaffected. Increased lactate production by lasalocid and monensin in the presence of protozoa was possibly due to inhibition of protozoal engulfment of starch. Thiopeptin had no effect on lactate production in the presence of protozoa but in the absence of protozoa lactate production was inhibited. Similar antibiotic responses were observed at different starch amounts (0.5, 1.5 and 3.0 g) and with starch types (soluble, corn and wheat) and with rumen fluid collected from defaunated cattle. Key words: Antibiotics, cattle, rumen, starch, fermentation, lactic acid
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Jalč, D., i M. Čertík. "Effect of microbial oil, monensin and fumarate on rumen fermentation in artificial rumen". Czech Journal of Animal Science 50, No. 10 (11.12.2011): 467–72. http://dx.doi.org/10.17221/4238-cjas.
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The objective of this study was to investigate the effect of microbial oil on rumen fermentation of a diet composed of 60% hay and 40% barley in an artificial rumen (Rusitec). Microbial oil (MO) was produced by the fungus Thamnidium elegans. This fungus grew on the wheat bran/spent malt grains (3:1) mixture. The fatty acid composition of microbial oil was as follows: 0.7% C<sub>14:0</sub>, 15.4% C<sub>16:0</sub>, 10.1% C<sub>18:0</sub>, 50.9% C<sub>18:1</sub>, 13.9% C<sub>18:2</sub> and 8.4% C<sub>18:3</sub> (GLA, γ-linolenic acid). The effect of monensin MON (66 ppm) and fumarate FUM (6.25 mmol) with and without MO supplementation was also studied. The experiment in Rusitec lasted 11 days. After a stabilization period (5 days), MO was added to fermentation vessel V<sub>2</sub> (6 days), MON to fermentation vessel V<sub>3</sub> (6 days) and FUM to fermentation vessel V<sub>4 </sub>(6 days). MO was also added to V<sub>3</sub> and V<sub>4</sub> on the last day together with MON (V<sub>3</sub>) and FUM (V<sub>4</sub>). The fermentation vessel V<sub>1 </sub>served as control (without additives). The results showed that MO reduced (P < 0.05) mol% acetate and increased (P < 0.05) mol% propionate and n-butyrate. Methane production (mmol/day) was reduced numerically (NS). The efficiency of microbial synthesis (EMS) was also reduced numerically and nitrogen incorporated by the microflora (N<sub>M</sub>) was reduced significantly in MO supplementation. There were no differences in the rumen fermentation when MO was applied together with MON and FUM compared to the vessel where only MO was applied. No additive effect was observed in the relationship MO-ionophore or MO-FUM. Monensin and fumarate applied separately showed their typical effects on rumen fermentation in vitro.
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Moningkey, Sony A. E., R. A. V. Tuturoong i I. D. R. Lumenta. "PEMANFAATAN ISI RUMEN TERFERMENTASI CELLULOMONAS Sp SEBAGAI CAMPURAN PAKAN KOMPLIT TERNAK KELINCI". ZOOTEC 40, nr 1 (31.01.2020): 352. http://dx.doi.org/10.35792/zot.40.1.2020.28245.
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UTILIZATION OF FERMENTED RUMENT CONTENT WITH CELLULOMONAS SP IN MIXED COMPLETE FEED FOR RABBIT. Research conducted to learn how to use cattle rumen content by using fermentation processing techniques to enable this rumen to be used as rabbit feed. The material used in this study consisted of cattle rumen contents, starter Cellulomonas sp, rabbits, complete feed. This research consisted of two phase. The first study used an experimental method with a completely randomized design 4 preparations and 6 replications. The fermentation time consists of 0 hours, 24 hours, 48 hours and 72 hours. For the second study using an experimental method with randomized block design based on the initial body weight of rabbits. The treatment given is the level of use of the best fermented rumen contents in a complete feed ration. Variable which is translated as feed consumption, body weight gain and feed conversion. Research results The first stage of the P4 study sample (72 hours) as the best guideline is seen from the parameters of crude protein and crude fiber. The results of this study indicate that the use of feed using rumen fermentation (IRF) can increase feed consumption and weight gain. The conclusion of this study is the provision of 30% mixture of fermented rumen contents of Cellulomonas sp in complete feed produced the best results seen from the parameters of consumption, weight gain and feed conversion of rabbit.Keywords: Rumen contents, fermentation, Cellulomonas sp, complete feed, rabbits
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Walker, Charles E., James S. Drouillard i Tiruvoor G. Nagaraja. "Optaflexx1 affects rumen fermentation". Kansas Agricultural Experiment Station Research Reports, nr 1 (1.01.2007): 88–90. http://dx.doi.org/10.4148/2378-5977.1536.
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Castillo-González, AR, ME Burrola-Barraza, J. Domínguez-Viveros i A. Chávez-Martínez. "Rumen microorganisms and fermentation". Archivos de medicina veterinaria 46, nr 3 (2014): 349–61. http://dx.doi.org/10.4067/s0301-732x2014000300003.
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Banik, B. K., Z. Durmic, W. Erskine, K. Ghamkhar i C. Revell. "In vitro ruminal fermentation characteristics and methane production differ in selected key pasture species in Australia". Crop and Pasture Science 64, nr 9 (2013): 935. http://dx.doi.org/10.1071/cp13149.
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Thirteen current and potential pasture species in southern Australia were examined for differences in their nutritive values and in vitro rumen fermentation profiles, including methane production by rumen microbes, to assist in selection of pasture species for mitigation of methane emission from ruminant livestock. Plants were grown in a glasshouse and harvested at 7 and 11 weeks after sowing for in vitro batch fermentation, with nutritive values assessed at 11 weeks of growth. The pasture species tested differed significantly (P < 0.001) in methane production during in vitro rumen fermentation, with the lowest methane-producing species, Biserrula pelecinus L., producing 90% less methane (4 mL CH4 g–1 dry matter incubated) than the highest methane-producing species, Trifolium spumosum L. (51 mL CH4 g–1 dry matter incubated). Proxy nutritive values of species were found not to be useful predictors of plant fermentation characteristics or methane production. In conclusion, there were significant differences in fermentative traits, including methane production, among selected pasture species in Australia, indicating that the choice of fodder species may offer a way to reduce the impact on the environment from enteric fermentation.
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Rarumangkay, Jeni. "PENGARUH FERMENTASI ISI RUMEN SAPI DENGAN Trichoderma viride TERHADAP ENERGI METABOLIS PADA AYAM BROILER". ZOOTEC 35, nr 2 (15.07.2015): 312. http://dx.doi.org/10.35792/zot.35.2.2015.8569.
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THE EFFECT OF DRIED COW RUMEN FERMENTATION WITH TRICHODERMA VIRIDE ON METABOLIZABLE ENERGY VALUE OF BROILER. The purpose of this experiment was to determine the metabolizable energy of dried cow rumen. The experiment use dried cow rumen and dried cow rumen fermented Trichoderma viride during 9 days with 0,3% inoculum dose. The experiment use 18 six weeks old male broiler metabolizable energy parameter were analyzed with Wilcoxon test. The result of this experiment showed fermentation with Trichoderma viride could increase the metabolizable energy of dried cow rumen. Key word : Fermentation of dried cow rumen, broiler, metabolizable energy
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Nagadi, S., M. Herrero i N. S. Jessop. "Effect of frequency of ovine ruminal sampling on microbial activity and substrate fermentation". Proceedings of the British Society of Animal Science 1999 (1999): 154. http://dx.doi.org/10.1017/s1752756200003094.
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Food eaten by a ruminant firstly undergoes microbial fermentation within the rumen. Nutritionally important characteristics of the food are the rate and extent of fermentation of its carbohydrate fraction, which can both be estimated using the in vitro gas production technique. The single greatest source of uncontrolled variation in any in vitro rumen fermentation system is the rumen fluid; curves produced from gas production data were influenced significantly by the variation in microbial activity between days (Menke and Steingass, 1988; Beuvink et al, 1992). A more reliable measure of rumen fluid activity is needed. The objective of this study was to determine whether the frequency of sampling of rumen fluid affected the microbial activity and subsequent fermentation.
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Bagheri, M., G. R. Ghorbani, H. R. Rahmani i M. Khorvash. "Effect of yeast and mannan-oligosaccharides on in vitro fermentation of different substrates". Proceedings of the British Society of Animal Science 2009 (kwiecień 2009): 91. http://dx.doi.org/10.1017/s1752756200029306.
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Results of yeast effects on in vitro rumen fermentation are inconsistent (Sullivan et al., 1999; Yang et al., 2004). In addition, there is no data on the effect of mannan-oligosacchrides (MOS) and their interaction with yeast on rumen fermentation. This trial was conducted to study the effects of yeast and MOS on rumen fermentation of different substrates.
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Wilk, Martyna, Ewa Pecka-Kiełb, Jerzy Pastuszak, Muhammad Umair Asghar i Laura Mól. "Effects of Copper Sulfate and Encapsulated Copper Addition on In Vitro Rumen Fermentation and Methane Production". Agriculture 12, nr 11 (18.11.2022): 1943. http://dx.doi.org/10.3390/agriculture12111943.
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Copper is a microelement crucial for the proper functioning of animals’ metabolic processes. The function of copper in rumen fermentation processes and methanogenesis is not well analyzed. The aim of the study was to evaluate the different types of copper supplement, their rumen decomposition and effect on in vitro ruminal fermentation as well as methanogenesis. Two different copper additives were used in the experiment: CS—copper sulfate (CuSO4 × 5 H2O)—and EC—encapsulated copper (tribasic copper chloride and copper sulfate enclosed within a polysaccharide polymer coating). A total mixed ration without copper additive was used as a control (C). In vitro rumen fermentation was conducted, and fermentation profile, gas production and methanogenesis were evaluated. After 24 h of fermentation, the amount of copper in the rumen fluid was significantly higher in the CS group. EC was protected against rumen degradation to a greater extent. The type of used copper supplement affects rumen fermentation. However, the effect on methanogenesis is ambiguous. CS supplement increases rumen gas production but does not affect methanogenesis. The obtained results suggest that the EC supplement may reduce the risk of low-fat milk and may improve the economic indicators of milk production. An in vivo experiment is necessary to compare the obtained in vitro results with animal productivity.
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Newbold, C. J., R. J. Wallace i I. M. Nevison. "Influence of ionophores on in vitro fermentation by rumen fluid from sheep receiving yeast culture (Yeasacc; YC)". Proceedings of the British Society of Animal Production (1972) 1991 (marzec 1991): 78. http://dx.doi.org/10.1017/s0308229600020286.
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A wide range of compounds has been described which have the potential to improve animal production by manipulating the rumen fermentation. Prominent among these rumen modifiers are the ionophores. Ionophores, such as monensin and tetronasin, improve feed efficiency, partly by increasing the flow of amino-N from the rumen and partly by stimulating the production of propionate in the rumen with an associated reduction in the production of methane (Russell and Strobel, 1988). Recently there has been increasing interest in the use of yeast culture (YC) and other fungal preparation to modify the rumen fermentation. These products have been shown to increase bacterial numbers within the rumen with an associated increase in the breakdown of fibre and supply of microbial protein (Williams and Newbold, 1990). YC has also been reported to increase the production of propionate in the rumen. Little appears to be known about the effect a combination YC and an ionophore would have on the rumen fermentation. This study describes the effects of the ionophores monensin and tetronasin on the fermentation of hay by rumen fluid from sheep fed a basal diet with or without YC.
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Li, Jinhui, Hui Yan, Jiaxin Chen, Chunhui Duan, Yunxia Guo, Yueqin Liu, Yingjie Zhang i Shoukun Ji. "Correlation of Ruminal Fermentation Parameters and Rumen Bacterial Community by Comparing Those of the Goat, Sheep, and Cow In Vitro". Fermentation 8, nr 9 (28.08.2022): 427. http://dx.doi.org/10.3390/fermentation8090427.
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In this study, we aimed to establish the correlation between ruminal fermentation parameters and the bacterial community by comparing those of the goat, sheep, and cow, thus illustrating the main bacteria causing the difference in rumen fermentation among goats, sheep, and cows and providing a new idea for improving the feed digestibility of ruminants. Rumen fluid from goats (Taihang White cashmere goat, n = 6), sheep (Hu sheep, n = 6), and cows (Holstein cow, n = 6) was collected using oral intubation and immediately brought back to the laboratory for a fermentation test with the same total mixed ration (TMR) feed in vitro. The rumen bacterial composition was measured by high-throughput sequencing of 16S rRNA genes in the MiSeq platform, the gas production (GP) was recorded after 2, 4, 6, 8, 10, 12, 24, 36, and 48 h of fermentation, and the feed nutrient digestibility and the rumen fluid parameters were determined after 48 h of fermentation. The results showed that the 48 h GP of the sheep group was higher than that of the cow group (p < 0.05), and the theoretical maximum GP was higher than that of the goat and cow groups (p < 0.05). The organic matter digestibility (OMD), dry matter digestibility (DMD), crude protein digestibility (CPD), and gross energy digestibility (GED) of the sheep group were higher than those of the goat and cow groups (p < 0.05). The ammonia nitrogen (NH3-N), microbial protein (MCP), and total volatile fatty acids (TVFA) concentrations of the sheep group were higher than those of the other groups (p < 0.05), and the pH of the sheep group was lower than those of the other groups (p < 0.05). The 16S rRNA gene sequencing revealed that bacterial composition also differed in the rumens of the sheep, goat, and cow groups (ANOSIM, p < 0.05). We then used a random forest machine learning algorithm to establish models to predict the fermentation parameters by rumen bacterial composition, and the results showed that rumen bacterial composition could explain most of the ruminal fermentation parameter variation (66.56%, 56.13%, 65.75%, 80.85%, 61.30%, 4.59%, 1.41%, −3.13%, 34.76%, −25.62%, 2.73%, 60.74%, 76.23%, 47.48%, −13.2%, 80.16%, 4.15%, 69.03%, 32.29%, and 89.96% for 48 h GP, a (GP of quickly degraded part), b (GP of slowly degraded part), c (GP rate), a + b (theoretical maximum GP), DMD, OMD, GED, CPD, NDFD, ANDF, pH, NH3-N, MCP, acetic acid, propionic acid, butyric acid, valeric acid, TVFA, and A:P (acetic acid–propionic acid ratio), respectively). A correlation analysis revealed that Lactobacillus, Prevotellaceae_UCG-003, Selenomonas, Peptostreptococcus, and Olsenella significantly correlated with most in vitro fermentation parameters (p < 0.05). A comprehensive analysis showed that rumen fermentation parameters and bacterial composition differed in goats, sheep, and cows. The ruminal fermentation parameters of GP, a, b, c, a + b, pH, NH3-N, propionic acid, valeric acid, and A:P could be accurately predicted by rumen bacteria (explanation > 55% of variation), and the Lactobacillus, Prevotellaceae_UCG-003, Olsenella, Selenomonas, and Peptostreptococcus were the main bacteria that affected the in vitro fermentation parameters of goats, sheep, and cows.
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Candyrine, S. C. L., M. F. Jahromi, M. Ebrahimi, J. B. Liang, Y. M. Goh i N. Abdullah. "In vitro rumen fermentation characteristics of goat and sheep supplemented with polyunsaturated fatty acids". Animal Production Science 57, nr 8 (2017): 1607. http://dx.doi.org/10.1071/an15684.
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An in vitro gas-production study was conducted to compare differences in rumen fermentation characteristics and the effect of supplementation of 4% linseed oil as a source of polyunsaturated fatty acids on the rumen fermentation profile in rumen fluid collected from goats and sheep. Rumen fluid for each species was obtained from two male goats of ~18 months old and two sheep of similar sex and age fed the similar diet containing 30% alfalfa hay and 70% concentrates. The substrate used for the fermentation was alfalfa hay and concentrate mixture (30:70) without (control) and with addition of linseed oil. The experiment was a two (inoculums) × two (oil levels) factorial experiment, with five replicates per treatment, and was repeated once. Rumen fermentation characteristics, including pH, fermentation kinetics, in vitro organic matter digestibility (IVOMD), volatile fatty acid (VFA) production and microbial population were examined. Results of the study showed that gas-production rate (c), IVOMD, VFA production and population of total bacteria and two cellulolytic bacteria (Ruminococus albus and Butyrivibrio fibrisolvens) from rumen fluid of goat were significantly (P < 0.05) higher than those of samples from sheep. Irrespective of sources of inoculums, addition of oil did not affect fermentation capacity, IVOMD and total VFA production. The higher B. fibrisolvens population (associated with bio-hydrogenation) in rumen fluid of goat seems to suggest that polyunsaturated fatty acids are more prone to bio-hydrogention in the rumen of goat than in sheep. This assumption deserves further investigation.
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Yu, Jiangkun, Liyuan Cai, Jiacai Zhang, Ao Yang, Yanan Wang, Lei Zhang, Le Luo Guan i Desheng Qi. "Effects of Thymol Supplementation on Goat Rumen Fermentation and Rumen Microbiota In Vitro". Microorganisms 8, nr 8 (30.07.2020): 1160. http://dx.doi.org/10.3390/microorganisms8081160.
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This study was performed to explore the predominant responses of rumen microbiota with thymol supplementation as well as effective dose of thymol on rumen fermentation. Thymol at different concentrations, i.e., 0, 100 mg/L, 200 mg/L, and 400 mg/L (four groups × five replications) was applied for 24 h of fermentation in a rumen fluid incubation system. Illumina MiSeq sequencing was applied to investigate the ruminal microbes in addition to the examination of rumen fermentation. Thymol doses reached 200 mg/L and significantly decreased (p < 0.05) total gas production (TGP) and methane production; the production of total volatile fatty acids (VFA), propionate, and ammonia nitrogen, and the digestibility of dry matter and organic matter were apparently decreased (p < 0.05) when the thymol dose reached 400 mg/L. A thymol dose of 200 mg/L significantly affected (p < 0.05) the relative abundance of 14 genera of bacteria, three species of archaea, and two genera of protozoa. Network analysis showed that bacteria, archaea, and protozoa significantly correlated with methane production and VFA production. This study indicates an optimal dose of thymol at 200 mg/L to facilitate rumen fermentation, the critical roles of bacteria in rumen fermentation, and their interactions with the archaea and protozoa.
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Ungerfeld, Emilio M., M. Fernanda Aedo, Emilio D. Martínez i Marcelo Saldivia. "Inhibiting Methanogenesis in Rumen Batch Cultures Did Not Increase the Recovery of Metabolic Hydrogen in Microbial Amino Acids". Microorganisms 7, nr 5 (27.04.2019): 115. http://dx.doi.org/10.3390/microorganisms7050115.
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There is an interest in controlling rumen methanogenesis as an opportunity to both decrease the emissions of greenhouse gases and improve the energy efficiency of rumen fermentation. However, the effects of inhibiting rumen methanogenesis on fermentation are incompletely understood even in in vitro rumen cultures, as the recovery of metabolic hydrogen ([H]) in the main fermentation products consistently decreases with methanogenesis inhibition, evidencing the existence of unaccounted [H] sinks. We hypothesized that inhibiting methanogenesis in rumen batch cultures would redirect [H] towards microbial amino acids (AA) biosynthesis as an alternative [H] sink to methane (CH4). The objective of this experiment was to evaluate the effects of eight inhibitors of methanogenesis on digestion, fermentation and the production of microbial biomass and AA in rumen batch cultures growing on cellulose. Changes in the microbial community composition were also studied using denaturing gradient gel electrophoresis (DGGE). Inhibiting methanogenesis did not cause consistent changes in fermentation or the profile of AA, although the effects caused by the different inhibitors generally associated with the changes in the microbial community that they induced. Under the conditions of this experiment, inhibiting methanogenesis did not increase the importance of microbial AA synthesis as a [H] sink.
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YANG, H. J., H. ZHUANG, X. K. MENG, D. F. ZHANG i B. H. CAO. "Effect of melamine onin vitrorumen microbial growth, methane production and fermentation of Chinese wild rye hay and maize meal in binary mixtures". Journal of Agricultural Science 152, nr 4 (15.10.2013): 686–96. http://dx.doi.org/10.1017/s0021859613000725.
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SUMMARYThe effects of melamine on gas production (GP) kinetics, methane (CH4) production and fermentation of diets differing in forage content (low-forage (LF) diet: 200 g/kg and high-forage (HF) diet: 800 g/kg) by rumen micro-organismsin vitrowere studied using batch cultures. Rumen contents were collected from three Simmental×Luxi crossbred beef cattle. Melamine was added to the incubation bottles to achieve final concentration of 0 (control), 2, 6, 18, 54, 162 and 484 mg/kg of each diet. Cumulative GP was continuously measured in an automated gas recording instrument during 72 h of incubation, while fermentation gas end-products were collected to determine molar proportions of carbon dioxide (CO2), CH4and hydrogen gas (H2) in manually operated batch cultures. Differences in GP kinetics and fermentation gases were observed in response to the nature of the diets incubated. Although melamine addition did not affect GP kinetics and fermentation gas pattern compared to the control, the increase of melamine addition stimulated the yield of CH4by decreasing CO2, especially during the fermentation of the HF diet. The concentrations of ammonia nitrogen (N), amino acid N and microbial N in culture fluids were greater in the fermentation of LF- than HF diets, and these concentrations were increased by the increase of melamine addition after 72-h fermentation. The concentrations of total volatile fatty acids (VFA) were greater in HF than LF diets. The addition of melamine decreased total VFA concentrations and this response was greater in HF than LF diet fermentations. Melamine addition did not affect molar proportions of acetate, butyrate, propionate and valerate compared with the control; however, branched-chain VFA production, which was lower in the HF than the LF diet, was increased by the melamine addition, especially in the HF diet fermentation. The ratio of non-glucogenic to glucogenic acids was lower in the HF than the LF diet, but it was not affected by melamine addition. In brief, the greater reduction in the rate and extent of rumen fermentation found for the HF diet in comparison with the LF diet suggested that rumen fermentation rate and extentin vitrodepended mainly on the nature of the incubated substrate, and that they could be further inhibited by the increase of melamine addition.
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Hesni, V., A. Taghizadeh, H. Paya, H. Janmohamadi, G. A. Moghadam i N. Pirani. "Effect of monensin and lasalocid on rumen fermentation in sheep". Proceedings of the British Society of Animal Science 2007 (kwiecień 2007): 221. http://dx.doi.org/10.1017/s1752756200021244.
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Ionophores, consisting of monensin and lasalocid were reported to improve rumen fermentation and metabolism of ruminants when delivered in their respective proper dosage (Mass et al., 2001). However, the efficacy of ionophores in the manipulation of rumen fermentation has been shown to vary with diet. Ionophores may also inhibit ruminal amino acid deamination (Yang et al., 2003). The objective of this study was to determine of the effect of monensin and lasalocid on rumen fermentation characteristics.
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Qiu, Xinjun, Xiaoli Qin, Liming Chen, Zhiming Chen, Rikang Hao, Siyu Zhang, Shunran Yang i in. "Serum Biochemical Parameters, Rumen Fermentation, and Rumen Bacterial Communities Are Partly Driven by the Breed and Sex of Cattle When Fed High-Grain Diet". Microorganisms 10, nr 2 (30.01.2022): 323. http://dx.doi.org/10.3390/microorganisms10020323.
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Hybridization in bovines is practiced with the main aim of improving production performance, which may imply the microbial variations in the rumen from the parental breed cross to their progeny. Besides, the interactions of offspring breed with sex in terms of rumen bacteria are not clear. This study aims to evaluate the variations in rumen bacterial communities in different breeds and sexes, and the correlations among fattening performance, serum biochemical parameters, and rumen fermentation. Forty-two 19.2 ± 0.67-month-old beef cattle (390 ± 95 kg of initial body weight) comprising two genetic lines (Yiling and Angus × Yiling) and two sexes (heifers and steers) were raised under the same high-grain diet for 120 d. On the last two days, blood samples were collected from each animal via the jugular vein before morning feeding for analyzing serum biochemical parameters; rumen fluid samples were obtained via esophageal intubation 2 h after morning feeding for analyzing rumen fermentation parameters and bacterial communities. The results show that both breed and sex had a certain impact on fattening performance, serum biochemical parameters, and rumen fermentation. No differences in the diversity and structure of rumen bacterial communities were observed. Significant interactions (p < 0.05) of breed and sex were observed for Succinivibrionaceae UCG-002 and Prevotellaceae UCG-001. The relative abundances of the Rikenellaceae RC9 gut group, Prevotellaceae UCG-003, and Succinivibrio were different (p < 0.05) between breeds. Heifers had a higher (p = 0.008) relative abundance of the Rikenellaceae RC9 gut group than steers. Correlation analysis showed a significant relationship (p < 0.05) of rumen bacteria with serum biochemical parameters, rumen pH, and rumen fermentation patterns. Additionally, only two genera, Prevotellaceae UCG-003 and Prevotellaceae UCG-001, had positive correlations with feed efficiency. In conclusion, serum biochemical parameters, rumen fermentation, and rumen bacterial communities are partly driven by the breed and sex of cattle fed a high-grain diet.
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Calsamiglia, S., P. Cardozo, A. Ferret i A. Bach. "Changes in rumen microbial fermentation during acidosis are due to a combined effects of fermentation substrate and pH". Proceedings of the British Society of Animal Science 2007 (kwiecień 2007): 21. http://dx.doi.org/10.1017/s1752756200019244.
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The supply of high-concentrate diets results in the reduction in ruminal pH and the development of acidosis. Because the reduction of pH occurs at the same time as the diet is changed, the effects are confounded. For example, high-concentrate diets ferment towards propionate, and low rumen pH also results in greater propionate production. Which factor is responsible for the increase in rumen propionate? The objective of this study was to determine the effects of rumen pH and the type of diet (D) on rumen microbial fermentation, with the aim of developing simple mathematical model to describe these effects.
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Cone, J. W., i M. A. M. Rodrigues. "Protein fermentation characteristics in rumen fluid determined with the gas production technique". Proceedings of the British Society of Animal Science 2009 (kwiecień 2009): 192. http://dx.doi.org/10.1017/s1752756200030313.
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The gas production technique was developed to determine the fermentation kinetics of organic matter in rumen fluid. However, the gas production technique can be adapted for the determination of protein fermentation characteristics. To do that the buffer must be N-free. All the N coming with the rumen fluid must be incorporated into microbial mass. This can be done by supplying the buffered rumen fluid with an excess of fast fermentable carbohydrates. To prevent a too high input of N from the rumen fluid the rumen fluid can be diluted further compared to the standard 3 times (Cone et al., 1996). This makes N the limiting factor for fermentation and the obtained gas production profiles reflect the availability of N from the feed samples. The aim of the present study was to investigate if the adapted gas production technique is suitable to determine differences in protein availability in rumen fluid. The fermentation characteristics of N of 19 feed samples were determined using the adapted gas production technique. The amount of sample incubated, was that sufficient to provide 15 mg N. The results were compared with data of N degradation obtained with the nylon bag technique.
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Rinttilä, Teemu, Colm A. Moran i Juha Apajalahti. "DHA-Rich Aurantiochytrium Biomass, a Novel Dietary Supplement, Resists Degradation by Rumen Microbiota without Disrupting Microbial Activity". Applied Microbiology 2, nr 1 (7.01.2022): 53–72. http://dx.doi.org/10.3390/applmicrobiol2010004.
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We first sought to evaluate the effect of dietary supplementation with the docosahexaenoic acid (DHA)-rich microalgae, Aurantiochytrium limacinum (AURA), on rumen fermentation and the resistance of DHA to degradation and biohydrogenation by rumen microbes through ex vivo fermentation experiments. Subsequently, we sought to quantify the diet-derived DHA content of milk and the impact of AURA on microbial composition and metabolism in a pilot feeding trial with rumen-cannulated dairy cows. To achieve our aims, rumen fluid from cannulated cows was used as inoculum, and the effect of AURA inclusion on fermentation ex vivo was examined. At doses corresponding to the amount of AURA recommended for commercial production animals, only ~10% of DHA was degraded or biohydrogenated by rumen microorganisms. The results show that feeding with AURA had no effect on either total bacterial density or short-chain fatty acid production. Real-time quantitative PCR analysis of the rumen fluid samples collected during a seven-week in vivo trial revealed that microbes related to lactic acid metabolism and methanogenesis were significantly suppressed by the AURA-supplemented diet. The DHA concentration in milk increased over 25-fold with the AURA-supplemented diet and dropped by 30–40% within one week of washout. The addition of A. limacinum biomass to dairy cow diets resulted in positive effects on rumen microbial composition with no adverse effect on fermentation activity. AURA-derived DHA was stable, with only modest degradation in the rumen, and was successfully deposited in milk. This is the first study to investigate the effect of supplementing the diet of dairy cows with a protist-based biomass, namely, on important rumen fermentation parameters and on DHA deposition in milk, using a combination of ex vivo and in vivo approaches.
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Munyiva, Brenda, i Wahu Oyaya. "Effect of Rumen Fluid Dosage and Fermentation Time on Dissolved Protein Levels of Vegetable Waste Silage for Vannamei Shrimp Feed". International Journal Papier Advance and Scientific Review 2, nr 2 (7.10.2021): 20–24. http://dx.doi.org/10.47667/ijpasr.v2i2.110.
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Specifically, the goal of this research was to evaluate the dissolved protein content of vegetable waste generated during the incubation of rumen fluid for use in (Whiteleg) vannamei shrimp diet. The results of the analysis of the degree of protein hydrolysis of vegetable waste treated with the addition of rumen fluid enzymes and different fermentation times of rumen fluid revealed that the addition of rumen fluid enzymes and different fermentation times of rumen fluid had a statistically significant effect (p0.05) on the degree of protein hydrolysis of vegetable waste. But there was no significant difference in the length of fermentation time or the interaction between the dosage of rumen fluid and the length of time (p>0.05) between the two groups. Duncan's test of rumen fluid dosage revealed that the degree of hydrolysis at a 1 percent dose was considerably greater (p0.05) than at 2 percent and 3 percent doses, and that the degree of hydrolysis at a 3 percent dose was significantly lower than at 2 percent
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Durix, Andrée, C. Jean-Blain, H. P. Sallmann i J. P. Jouany. "Use of a semicontinuous culture system (RUSITEC) to study the metabolism of ethanol in the rumen and its effects on ruminal digestion". Canadian Journal of Animal Science 71, nr 1 (1.03.1991): 115–23. http://dx.doi.org/10.4141/cjas91-013.
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The metabolism of ethanol by rumen micro-organisms and its effects on rumen fermentations have been studied in vitro in a semicontinuous fermentor (RUSITEC). Ethanol introduced in the fermentor at 1, 4 or 8 g d−1 L−1 of rumen juice induced important qualitative and quantitative modifications in the fermentation outputs. Total VFA production was increased from 5 to 40% according to the diet and ethanol concentration. Relative proportions of VFA were modified: caproate concentration increased three times, propionate and isovalerate concentrations decreased significantly in most cases. Methane production was increased. Whatever the ethanol concentration and the type of diet, in vitro digestibility parameters and end-products from solid feedstuffs stayed practically unchanged by ethanol addition. Except with the lowest supply (1 g d−1 L−1), ethanol induced an uncoupling effect on the metabolism of rumen bacteria and decreased biomass production. Radioactivity of 2-14C ethanol was recovered mainly in acetate (77–80%). Microbial transformation of ethanol in RUSITEC was limited regardless of ethanol concentration. Ethanol had a negligible effect on the digestibility of solid feedstuffs but induced consistent qualitative changes in rumen fermentations. Key word: Ethanol, metabolism, rumen, semicontinuous culture
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Guo, Wei, Jolet K. van Niekerk, Mi Zhou i Michael A. Steele. "PSIX-32 Assessment of Mucosa-associated Microbiota in the Colon and Rumen of Dairy Calves Fed High Plane of Milk and during Weaning Transition". Journal of Animal Science 98, Supplement_4 (3.11.2020): 311. http://dx.doi.org/10.1093/jas/skaa278.554.
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Abstract The nature of weaning is considered as the most stressful period experienced by dairy calves and has been known to negatively affect calf growth and intake. The objectives of this study were to investigate the shift of rumen and colon mucosa-associated microbiota in dairy calves fed with a high plane of milk pre- and post-weaning, and whether such shifts are associated with rumen fermentation and growth performance. Six Holstein dairy bull calves were ruminally cannulated at the second week of life followed by weaning at the end of week 6. Ruminal and colon tissue samples were biopsied at the end of week 5, 7 and 12 to determine the bacterial community changes, and rumen content was collected at the same time to measure rumen fermentation parameters. QIIME2 was used to analyze microbial profiles and R studio software was used to determine the microbial changes and its relationship with phenotypic measures. Age-dependent profiles were observed for the colon mucosa associated microbiota but not for rumen mucosa associated microbiota, indicating that varied responses to weaning transition exists between colon and rumen mucosa associated microbiota. As calves aged, higher abundance of taxa such as Delftia (associated with hexadecanoic acid production) and lower abundance of Veillonella (associated with short-chain organic acids utilization) were detected in the colon mucosa. The rumen also displayed taxonomic changes with decreases in Pseudobutyrivibrio in older calves. In addition, we identified significantly positive correlations between Prevotella associated with rumen mucosa and average daily gain, Isovalerate and Isobutyrate, suggesting that this genus may promote rumen fermentation during weaning transition. Our findings suggest that weaning transition affects rumen and colon mucosa-associated bacteria, and some of the rumen mucosa-associated microbiota promote rumen fermentation during this period, which may have long-term effects on animal health and productivity.
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Guo, Yanxia, Faiz-ul Hassan, Mengwei Li, Huade Xie, Lijuan Peng, Zhenhua Tang i Chengjian Yang. "Effect of Sodium Nitrate and Cysteamine on In Vitro Ruminal Fermentation, Amino Acid Metabolism and Microbiota in Buffalo". Microorganisms 10, nr 10 (14.10.2022): 2038. http://dx.doi.org/10.3390/microorganisms10102038.
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Nitrate is used as a methane inhibitor while cysteamine is considered as a growth promoter in ruminants. The present study evaluated the effect of sodium nitrate and cysteamine on methane (CH4) production, rumen fermentation, amino acid (AA) metabolism, and rumen microbiota in a low protein diet. Four treatments containing a 0.5 g of substrate were supplemented with 1 mg/mL sodium nitrate (SN), 100 ppm cysteamine hydrochloride (CS), and a combination of SN 1 mg/mL and CS 100 ppm (CS+SN), and a control (no additive) were applied in a completely randomized design. Each treatment group had five replicates. Two experimental runs using in vitro batch culture technique were performed for two consecutive weeks. Total gas and CH4 production were measured in each fermentation bottle at 3, 6, 9, 12, 24, 48, and 72 h of incubation. The results showed that SN and CS+SN reduced the production of total gas and CH4, increased the rumen pH, acetate, acetate to propionate ratio (A/P), and microbial protein (MCP) contents (p < 0.05), but decreased other volatile fatty acids (VFA) and total VFA (p = 0.001). The CS had no effect on CH4 production and rumen fermentation parameters except for increasing A/P. The CSN increased the populations of total bacteria, fungi, and methanogens but decreased the diversity and richness of rumen microorganisms. In conclusion, CS+SN exhibited a positive effect on rumen fermentation by increasing the number of fiber degrading and hydrogen-utilizing bacteria, with a desirable impact on rumen fermentation while reducing total gas and CH4 production.
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Wei, Xiao, Kehui Ouyang, Tanghui Long, Zuogui Liu, Yanjiao Li i Qinghua Qiu. "Dynamic Variations in Rumen Fermentation Characteristics and Bacterial Community Composition during In Vitro Fermentation". Fermentation 8, nr 6 (14.06.2022): 276. http://dx.doi.org/10.3390/fermentation8060276.
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This study aimed to explore the dynamic variations of rumen fermentation characteristics and bacterial community composition during a 24 h in vitro fermentation. A total of twenty-three samples were collected from original rumen fluid (ORF, n = 3), fermentation at 12 h (R12, n = 10), and fermentation at 24 h (R24, n = 10). Results showed that gas production, concentrations of microbial crude protein, ammonia nitrogen, and individual volatile fatty acids (VFA), as well as total VFA and branched-chain VFA concentrations, were higher in R24 when compared with R12 (p < 0.05). However, no significant differences were observed in acetate to propionate ratio and fermentation efficiency between R12 and R24 (p > 0.05). Bacterial diversity analysis found that Shannon index and Simpson index were higher in R24 (p < 0.05), and obvious clusters were observed in rumen bacterial community between R12 and R24. Taxonomic analysis at the phylum level showed that the abundances of Proteobacteria and Fibrobacteres were higher in R12 than that in R24, and inverse results were observed in Bacteroidetes, Firmicutes, Cyanobacteria, Verrucomicrobia, Lentisphaerae, and Synergistetes abundances. Taxonomic analysis at the genus level revealed that the abundances of Rikenellaceae RC9 gut group, Succiniclasticum, Prevotellaceae UCG-003, Christensenellaceae R-7 group, Ruminococcaceae UCG-002, Veillonellaceae UCG-001, and Ruminococcaceae NK4A214 group were higher in R24, whereas higher abundances of Succinivibrionaceae UCG-002, Ruminobacter, and Fibrobacter, were found in R12. Correlation analysis revealed the negative associations between gas production and abundances of Proteobacteria, Succinivibrionaceae UCG-002, and Ruminobacter. Moreover, the abundances of Firmicutes, Rikenellaceae RC9 gut group, Christensenellaceae R-7 group, and Ruminococcaceae UCG-002 positively correlated with VFA production. These results indicate that both rumen fermentation characteristics and bacterial community composition were dynamic during in vitro fermentation, whereas the fermentation pattern, efficiency, and bacterial richness remained similar. This study provide insight into the dynamics of rumen fermentation characteristics and bacterial composition during in vitro fermentation. This study may also provide a reference for decision-making for the sampling time point when conducting an in vitro fermentation for bacterial community investigation.
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Qian, Wenxi, ZhiPeng Li, Weiping Ao, Guangyong Zhao, Guangyu Li i JianPing Wu. "Bacterial community composition and fermentation in the rumen of Xinjiang brown cattle (Bos taurus), Tarim red deer (Cervus elaphus yarkandensis), and Karakul sheep (Ovis aries)". Canadian Journal of Microbiology 63, nr 5 (maj 2017): 375–83. http://dx.doi.org/10.1139/cjm-2016-0596.
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The rumen microbiota plays a major role in the metabolism and absorption of indigestible food sources. Xinjiang brown cattle (Bos taurus), Tarim red deer (Cervus elaphus yarkandensis), and Karakul sheep (Ovis aries) are important ruminant species for animal husbandry in the Tarim Basin. However, the microbiota and rumen fermentation of these animals are poorly understood. Here, we apply high-throughput sequencing to examine the bacterial community in the rumen of cattle, red deer, and sheep and measured rumen fermentation products. Overall, 548 218 high-quality sequences were obtained and then classified into 6034 operational taxonomic units. Prevotella spp., Succiniclasticum spp., and unclassified bacteria within the families Succinivibrionaceae, Lachnospiraceae, and Veillonellaceae were the dominant bacteria in the rumen across the 3 hosts. Principal coordinate analysis identified significant differences in the bacterial communities across the 3 hosts. Pseudobutyrivibrio spp., Oscillospira spp., and Prevotella spp. were more prevalent in the rumen of the cattle, red deer, and sheep, respectively. Among the 3 hosts, the red deer rumen had the greatest amounts of acetate and butyrate and the lowest pH value. These results showed that Prevotella spp. are the dominant bacteria in the rumen of the cattle, red deer, and sheep, providing new insight into the rumen fermentation of ruminants distributed in the Tarim Basin.
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Ouda, J. O., C. J. Newbold, S. Lopez, N. Nelson, A. R. Moss, R. J. Wallace i H. Omed. "The effect of acrylate and fumarate on fermentation and methane production in the rumen simulating fermentor (Rusitec)". Proceedings of the British Society of Animal Science 1999 (1999): 37. http://dx.doi.org/10.1017/s1752756200001927.
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Methane formation in the rumen represents a substantial loss of energy to the animal and is also a major source of greenhouse gas emissions from agriculture. Methanogenesis is the main means of disposal of hydrogen during rumen fermentation. The formation of propionate represents an alternative route of hydrogen disposal, providing sufficient propionate precursors are available. Theoretically, adding propionate precursors should stimulate propionate production and decrease methane production. In the present experiment, the effects of two potential precursors of propionate, fumarate and acrylate, on rumen fermentation and methane production were investigated in a rumen simulating fermentor.
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TKALCIC, SUZANA, CATHY A. BROWN, BARRY G. HARMON, ANANT V. JAIN, ERIC P. O. MUELLER, ANDREW PARKS, KAREN L. JACOBSEN, SCOTT A. MARTIN, TONG ZHAO i MICHAEL P. DOYLE. "Effects of Diet on Rumen Proliferation and Fecal Shedding of Escherichia coli O157:H7 in Calves". Journal of Food Protection 63, nr 12 (1.12.2000): 1630–36. http://dx.doi.org/10.4315/0362-028x-63.12.1630.
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Calves inoculated with Escherichia coli O157:H7 and fed either a high-roughage or high-concentrate diet were evaluated for rumen proliferation and fecal shedding of E. coli O157:H7. Calves fed the high-roughage diet had lower mean rumen volatile fatty acid concentrations and higher rumen pH values than did calves fed the high-concentrate diet. Despite these differences in rumen conditions, the calves fed the high-roughage diet did not have greater rumen populations of E. coli O157: H7 and did not exhibit increased or longer fecal shedding compared with the calves fed the high-concentrate diet. Two calves shedding the highest mean concentrations of E. coli O157:H7 were both fed the high-concentrate diet. There was a significant (P < 0.05) positive correlation between fecal shedding and rumen volatile fatty acid concentration in calves fed a high-concentrate diet. The effects of diet on E. coli O157:H7 proliferation and acid resistance were investigated using an in vitro rumen fermentation system. Rumen fluid collected from steers fed a high-roughage diet, but not from steers fed a high-concentrate diet, supported the proliferation of E. coli O157:H7. Rumen fluid from steers fed a high-concentrate diet rapidly induced acid resistance in E. coli O157:H7. The impact of diet on fecal shedding of E. coli O157:H7 is still unclear and may depend on dietary effects on fermentation in the colon and on diet-induced changes in the resident microflora. However, rapid development of acid tolerance by E. coli O157:H7 in the rumens of calves fed high-concentrate diets, allowing larger populations to survive passage through the acidic abomasum to proliferate in the colon, may be one factor that influences fecal shedding in cattle on feed.
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McAllister, T. A., i C. J. Newbold. "Redirecting rumen fermentation to reduce methanogenesis". Australian Journal of Experimental Agriculture 48, nr 2 (2008): 7. http://dx.doi.org/10.1071/ea07218.
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Methane production in ruminants has received global attention in relation to its contribution to the greenhouse gas effect and global warming. In the last two decades, research programs in Europe, Oceania and North America have explored a variety of approaches to redirecting reducing equivalents towards other reductive substrates as a means of decreasing methane production in ruminants. Some approaches such as vaccination, biocontrols (bacteriophage, bacteriocins) and chemical inhibitors directly target methanogens. Other approaches, such as defaunation, diet manipulations including various plant extracts or organic acids, and promotion of acetogenic populations, seek to lower the supply of metabolic hydrogen to methanogens. The microbial ecology of the rumen ecosystem is exceedingly complex and the ability of this system to efficiently convert complex carbohydrates to fermentable sugars is in part due to the effective disposal of H2 through reduction of CO2 to methane by methanogens. Although methane production can be inhibited for short periods, the ecology of the system is such that it frequently reverts back to initial levels of methane production though a variety of adaptive mechanisms. Hydrogen flow in the rumen can be modelled stoichiometrically, but accounting for H2 by direct measurement of reduced substrates often does not concur with the predictions of stoichiometric models. Clearly, substantial gaps remain in our knowledge of the intricacies of hydrogen flow within the ruminal ecosystem. Further characterisation of the fundamental microbial biochemistry of hydrogen generation and methane production in the rumen may provide insight for development of effective strategies for reducing methane emissions from ruminants.
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McCann, Joshua C., Ahmed A. Elolimy i Juan J. Loor. "Rumen Microbiome, Probiotics, and Fermentation Additives". Veterinary Clinics of North America: Food Animal Practice 33, nr 3 (listopad 2017): 539–53. http://dx.doi.org/10.1016/j.cvfa.2017.06.009.
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Hart, K. J., D. R. Yáñez-Ruiz, S. M. Duval, N. R. McEwan i C. J. Newbold. "Plant extracts to manipulate rumen fermentation". Animal Feed Science and Technology 147, nr 1-3 (listopad 2008): 8–35. http://dx.doi.org/10.1016/j.anifeedsci.2007.09.007.
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Fakhri, S., A. R. Moss, D. I. Givens i E. Owen. "Comparison of four in vitro gas production methods to study rumen fermentation kinetics of starch rich feeds". Proceedings of the British Society of Animal Science 1997 (1997): 196. http://dx.doi.org/10.1017/s1752756200596379.
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Recently, the automatic in vitro gas production techniques (e.g. Cone. 1994; Theodorou et al., 1994) have been developed to study rumen fermentation kinetics. Many approaches have been taken. This work investigates the suitability of different methods for estimating the rumen fermentation of two starch rich feedstuffs.
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Wang, Mengzhi. "In Vitro Fermentation". Fermentation 9, nr 2 (19.01.2023): 86. http://dx.doi.org/10.3390/fermentation9020086.
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The rumen of ruminants, as well as the colon of monogastric animals, are inhabited by over one trillion bacteria, fungi, and protozoa, and these are emerging as critical regulators in dietary micronutrients and animal health [...]
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Astuti, W. D., Y. Widyastuti, E. Wina, S. Suharti, R. Ridwan i K. G. Wiryawan. "Survival of Lactobacillus plantarumU40 on the in vitro rumen fermentation quantified with real-time PCR". Journal of the Indonesian Tropical Animal Agriculture 43, nr 2 (24.05.2018): 184. http://dx.doi.org/10.14710/jitaa.43.2.184-192.
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The objective of this study was to evaluate the survival of L. plantarumU40 quantified with real-time PCR during in vitro rumen fermentation. The experiment was arranged in a randomized block design with 3 treatments and 4 replications. Treatments were control, rumen fermentation inoculated with L. plantarumU40and L. plantarumU40 + glucose solution. Population of L. plantarum U40 was higher at inoculation treatment. After 8 hours incubation, glucose addition tended to decrease L. plantarum U40 population. Control treatment showed lowest population of L. plantarum U40 along in vitro fermentation compared with other treatment. Inoculation of L. plantarumU40 significantly (p<0.05) increased population of LAB until 12 hours incubation compared with control. Control treatment had highest pH at all incubation time. Glucose addition significantly (P<0.05) decreased final rumen pH (24 hours) (6.30), compared with control treatment (6.85). Inoculation of L. plantarum U40 with glucose addition significantly (P<0.05)increased propionic acid, decreased acetic acid and A/P ratio compared with other treatments. Lactobacillus plantarum U40 without glucose addition did not affect propionic acid production significantly. As conclusion, Lactobacillus plantarum U40 can survive in rumen fluid and changes rumen fermentation when glucose is added as carbon source.
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Hussain, A., i E. L. Miller. "Effect of supplementation of sucrose and lactose with sodium bicarbonate on rumen metabolism and microbial protein synthesis in sheep". Proceedings of the British Society of Animal Science 1999 (1999): 28. http://dx.doi.org/10.1017/s1752756200001836.
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Sucrose and lactose are used as energy supplements in ruminant diets. In our previous study (Hussain and Miller, 1998) lactose maintained a higher rumen pH, increased organic matter and neutral detergent fibre digestion in the rumen, reduced the number of rumen protozoa and increased microbial (bacterial) protein flow to the duodenum compared with sucrose. However, it was not clear whether the effect of lactose on pH or on reduction of protozoa was the main factor increasing rumen fermentation and microbial protein flow. The objective of the present study was to examine the effect of isoenergetic amounts of sucrose and lactose as supplements (equivalent to 50 g glucose) on rumen fermentation and metabolism in the presence of sodium bicarbonate to maintain rumen pH.
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CIESLAK, A., P. ZMORA, A. STOCHMAL, L. PECIO, W. OLESZEK, E. PERS-KAMCZYC, J. SZCZECHOWIAK, A. NOWAK i M. SZUMACHER-STRABEL. "Rumen antimethanogenic effect ofSaponaria officinalisL. phytochemicalsin vitro". Journal of Agricultural Science 152, nr 6 (8.04.2014): 981–93. http://dx.doi.org/10.1017/s0021859614000239.
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SUMMARYAlthough the effect of saponins or saponin-containing plants on rumen microorganisms and rumen fermentation has been intensively investigated, this issue still requires special attention. Many of the phenomena occurring in the rumen related to dietary saponin supplementation are still not fully understood.Saponaria officinalisis a triterpenoid saponin-containing plant; thus, the aim of the present study was to evaluate the effect ofS. officinalisL. powdered root, methanolic extract of theS. officinalisroot (SOR) and the effect of the separated fractions (polysaccharides, saponins and phenolics) ofS. officinalison rumen methanogenesis, microbial population and rumen fermentation characteristics in anin vitrobatch culture fermentation system. The powdered root (raw plant material) andS. officinalisextract (SOE) decreasedin vitromethane production and consequently reduced the microbial population in a dose-dependent manner. The inhibition of methanogenesis was accompanied by changes in the volatile fatty acids profile.In vitrodry matter digestibility was not affected by any of the secondary compounds applied. The highest applied doses of SOE caused a higher reduction in methanogenesis (33·5v. 14·4%) than the highest doses of powdered root form. Such results suggest that the basic components of the SOR could interact with phytochemicals or that the phytochemicals became physically less available for microbiota, resulting in a decreased antimethanogenic activity of the powdered rootv. the extract. Among all the fractions selected, the saponin fraction exerted the greatest impact on ruminal fermentation. In conclusion, saponins decreased methane production by 29% in comparison with the control. This decrease was related to the reduction in protozoa and methanogen counts. It is proposed thatS. officinalishas the potential to inhibit rumen methanogenesis without affecting rumen fermentation adversely.
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Kingston-Smith, Alison H., Joan E. Edwards, Sharon A. Huws, Eun J. Kim i Michael Abberton. "Plant-based strategies towards minimising ‘livestock's long shadow’". Proceedings of the Nutrition Society 69, nr 4 (4.08.2010): 613–20. http://dx.doi.org/10.1017/s0029665110001953.
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Ruminant farming is an important component of the human food chain. Ruminants can use offtake from land unsuitable for cereal crop cultivation via interaction with the diverse microbial population in their rumens. The rumen is a continuous flow fermenter for the digestion of ligno-cellulose, with microbial protein and fermentation end-products incorporated by the animal directly or during post-ruminal digestion. However, ruminal fermentation is inefficient in capturing the nutrient resource presented, resulting in environmental pollution and generation of greenhouse gases. Methane is generated as a consequence of ruminal fermentation and poor retention of ingested forage nitrogen causes nitrogenous pollution of water and land and contributes to the generation of nitrous oxide. One possible cause is the imbalanced provision of dietary substrates to the rumen micro-organisms. Deamination of amino acids by ammonia-producing bacteria liberates ammonia which can be assimilated by the rumen bacteria and used for microbial protein synthesis. However, when carbohydrate is limiting, microbial growth is slow, meaning low demand for ammonia for microbial protein synthesis and excretion of the excess. Protein utilisation can therefore be improved by increasing the availability of readily fermentable sugars in forage or by making protein unavailable for proteolysis through complexing with plant secondary products. Alternatively, realisation that grazing cattle ingest living cells has led to the discovery that plant cells undergo endogenous, stress-mediated protein degradation due to the exposure to rumen conditions. This presents the opportunity to decrease the environmental impact of livestock farming by using decreased proteolysis as a selection tool for the development of improved pasture grass varieties.
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Li, Qin, Yan Tu, Tao Ma, Kai Cui, Jianxin Zhang, Qiyu Diao i Yanliang Bi. "Effects of Two Feeding Patterns on Growth Performance, Rumen Fermentation Parameters, and Bacterial Community Composition in Yak Calves". Microorganisms 11, nr 3 (24.02.2023): 576. http://dx.doi.org/10.3390/microorganisms11030576.
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The health of young ruminants is highly dependent on early rumen microbial colonization. In this study, the effects of milk replacer on growth performance, rumen fermentation, and the rumen microflora in yak calves were evaluated. Sixty yak calves (body weight = 22.5 ± 0.95 kg, age = 30 ± 1 d) were assigned to the CON group (breastfed) or TRT group (milk replacer fed) and evaluated over 120 d. At 120 d, ruminal fluid samples were collected from 14 calves and then conducted for rumen fermentation and microbiota analyses. There was no difference in growth performance; however, calf survival was higher in the TRT group than in the CON group. The concentration of total volatile fatty acids and the molar proportion of butyric acid and lactic acid were increased with milk replacer feed in the TRT group (p < 0.05), but iso-valeric acid concentration was highest in the CON group (p < 0.05). Firmicutes and Bacteroidetes were the most dominant phyla in the CON and TRT groups, respectively. In the TRT group, Bacteroidetes, Prevotellaceae, Bacteroidia, Bacteroidetes, and Prevotella_1 were the dominant flora in the rumen of calves. The relative abundances of various taxa were correlated with rumen fermentation parameters; the relative abundance of Quinella and iso-butyrate levels were positively correlated (r = 0.57). The relative abundances of the Christensenellaceae_R-7_group and A/P were positively correlated (r = 0.57). In summary, milk replacer is conducive to the development of the rumen microflora, the establishment of rumen fermentation function, and the implementation of early weaning in yaks.
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McKain, N., C. J. Newbold i R. J. Wallace. "Combined effects of aspergillus oryzae fermentation extract (amaferm; ao) and monensin on fermentation in the rumen simulation technique (rusitec)". Proceedings of the British Society of Animal Production (1972) 1991 (marzec 1991): 112. http://dx.doi.org/10.1017/s0308229600020626.
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Monensin, an ionophore, and AO, a fungal feed additive, are growth promoters which modify rumen fermentation. Previous studies have shown that monensin increased propionate, the efficiency of bacterial growth and the flow of protein from the rumen and decreased methane production and fibre digestion (Schelling, 1984), whereas AO increased bacterial numbers and fibre digestion and lowered methane production (Frumholtzet al., 1989; Fondevilaet al., 1990). The aim of the present study was to determine, using Rusitec, the effects on rumen fermentation of combining these additives.
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Xue, Ligang, Shuyi Zhou, Dan Wang, Fangyu Zhang, Junfeng Li i Liyuan Cai. "The Low Dose of Saccharomyces cerevisiae Is Beneficial for Rumen Fermentation (Both In Vivo and In Vitro) and the Growth Performance of Heat-Stressed Goats". Microorganisms 10, nr 10 (20.09.2022): 1877. http://dx.doi.org/10.3390/microorganisms10101877.
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This study aimed to investigate the effects of Saccharomyces cerevisiae on rumen fermentation and the growth performance of heat-stressed goats. The fermentation experiment was conducted using Saccharomyces cerevisiae added at 0‰ (HS1), 0.30‰ (SC1), 0.60‰ (SC2), and 1.20‰ (SC3) of the dry matter (DM) weight of the basal diet. The results showed that supplementing with 0.60‰ (SC2) could increase the pH, acetic acid to propionic acid ratio, the concentrations of ammonia nitrogen, total volatile fatty acids, acetic acid, propionic acid, butyric acid, and the degradability of DM, neutral detergent fiber, and acid detergent fiber in rumen fluids of heat-stressed goats. In the feeding experiment, twelve heat-stressed goats were assigned to a 4 × 4 Latin square experimental design, and the Saccharomyces cerevisiae supplement levels are similar to the fermentation experiment above. Similar effects on rumen fermentation and digestibility parameters were obtained with a supplement with 0.60‰ of Saccharomyces cerevisiae (SC2A) compared to the fermentation trial. Moreover, in the SC2A group, the DM intake and average daily gain also increased significantly compared with other groups. These results suggested that a low dose of Saccharomyces cerevisiae can still effectively improve the rumen fermentation and growth performance of heat-stressed goats.
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Newbold, C. J., i R. J. Wallace. "The effect of yeast and distillery by-products on the fermentation in the rumen simulation technique (rusitec)". Proceedings of the British Society of Animal Production (1972) 1992 (marzec 1992): 210. http://dx.doi.org/10.1017/s0308229600023199.
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The practice of adding low levels of non-commensal yeast and fungi to ruminant diets is increasingly gaining acceptance as a means of manipulating rumen fermentation to benefit production. Reported benefits include an increased degradability of forages in the rumen and an improved flow of microbial protein from the rumen (Williams and Newbold, 1990).Distillery by-products, such as pot ale syrup, are commonly included in ruminant diets as an energy source. However, pot ale syrup contains a substantial number of yeast cells. The aim of the present study was to establish if different yeasts and yeast-containing by-products had similar effects on rumen fermentation to those found with a commercial yeast culture feed additive.Two commercial Saccharomyces cerevisiae preparations (Alkosel, Alko Biotechnology, Finland and Yea-sacc, Alltech, UK), active dried baker's yeast (United Distillers, UK) and two pot ale syrups from the Inchgower and Dailuaine distilleries were compared for their effects on the fermentation in the rumen simulation technique (Rusitec).
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Rabee, Alaa Emara, Khalid Z. Kewan, Hassan M. El Shaer, Mebarek Lamara i Ebrahim A. Sabra. "Effect of olive and date palm by-products on rumen methanogenic community in Barki sheep". AIMS Microbiology 8, nr 1 (2022): 26–41. http://dx.doi.org/10.3934/microbiol.2022003.
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<abstract> <p>Rumen methanogens prevent the accumulation of fermentation gases in the rumen and generate methane that increases global warming and represents a loss in animals' gross energy. Non-traditional feed resources such as the by-products of date palm (<italic>Phoenix dactylifera</italic>) and olive (<italic>Olea europaea</italic>) trees have received attention to be used in animal feeding. This study evaluated the impact of non-traditional feed resources including olive cake (OC), discarded dates (DD), and date palm frond (DPF) in sheep diet on rumen fermentation, diversity and relative abundance of rumen methanogens. Nine adult rams were assigned to three equal groups and fed three diets: traditional concentrates mixture (S1); non-traditional concentrate mixture (S2) based on DD and OC; and (S3) composed of the same S2 concentrate supplemented with DPF as a roughage part. The results showed that rumen pH was higher with S3 diet than the other two diets. However, the S1 diet showed the highest values of total volatile fatty acids (TVFA) and rumen ammonia. In addition, the proportions of acetic and butyric acids were increased, whereas propionic acid declined in S2 and S3 compared to the S1 diet. Rumen methanogens were dominated by <italic>Methanobrevibacter</italic> that showed a numeric decline by including DD, OC, and DPF in the animal diets. Principal component analysis (PCA) based on rumen fermentation parameters and relative abundances of methanogens genera showed three distinct clusters. Also, positive and negative correlations were revealed between methanogens genera and rumen metabolites. This study expands the knowledge regarding the effect of agricultural byproducts on rumen fermentation and the methanogenic community.</p> </abstract>
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Qiu, Qinghua, Chaoyu Gao, Muhammad Aziz ur Rahman, Binghai Cao i Huawei Su. "Digestive Ability, Physiological Characteristics, and Rumen Bacterial Community of Holstein Finishing Steers in Response to Three Nutrient Density Diets as Fattening Phases Advanced". Microorganisms 8, nr 3 (27.02.2020): 335. http://dx.doi.org/10.3390/microorganisms8030335.
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The aim of this study is to track the dynamic alterations in nutrient intake and digestion, rumen fermentation and plasma metabolic characteristics, and rumen bacterial community of Holstein finishing steers in response to three nutrient density diets as fattening phases advanced. A total of eighteen Holstein steers were randomly allocated into three nutrient density groups and steers in each group were fed under a three-phase fattening strategy, with nutrient density increased in each group when fattening phase advanced. Results showed that both fattening phase and dietary nutrient density significantly influenced the nutrient digestion, most of the rumen fermentation parameters, and part of bacteria at phylum and genus levels. Individually, dietary nutrient density affected the concentrations of plasma alanine aminotransferase and urea N, bacterial richness and evenness. All determined nutrient intake and plasma biochemical parameters, except for alanine aminotransferase and triglyceride, differed among fattening phases. Spearman correlation analysis revealed strong correlations between fiber intake and bacterial richness and evenness, rumen fermentation characteristics and certain bacteria. Moreover, Patescibacteria abundance was positively correlated with ambient temperature and plasma total protein. These results indicate that rumen fermentation and nutrient digestion were influenced by both dietary nutrient density and fattening phase, and these influences were regulated by certain rumen bacterial community and ruminal bacteria may be affected simultaneously by ambient temperature. This study may provide insights into diet optimization and potentially adaptive mechanism of rumen bacterial community in response to fattening phases and gradually climatic change.
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Xiao, Jianxin, Tianyu Chen, Gibson Maswayi Alugongo, Muhammad Zahoor Khan, Tingting Li, Jing Ma, Shuai Liu i in. "Effect of the Length of Oat Hay on Growth Performance, Health Status, Behavior Parameters and Rumen Fermentation of Holstein Female Calves". Metabolites 11, nr 12 (20.12.2021): 890. http://dx.doi.org/10.3390/metabo11120890.
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The aim of this study was to evaluate the effect of the length of oat hay on the performance, health, behavior, and rumen fermentation of dairy calves. For this purpose, two hundred and ten healthy two-day-old Holstein dairy calves were randomly allocated into three groups: basic diet (calf starter) without hay (CON), or a basic diet with oat hay at either long (OL: 10–12 cm) or short (OS: 3–5 cm) length cut. The basic diet was fed from day 4, while the hay was offered from day 14. All calves were weaned at day 56 and remained in their individual hutches till the end of the trial (day 70). Calf starter intake and fecal scores were recorded daily. Bodyweight, body size, and rumen fluid samples were collected biweekly before weaning and weekly after weaning. Overall, providing oat hay (OS and OL) in the diet increased the body weight, starter intake, and average daily gain compared to the CON group. Similarly, feeding oat hay improved rumen fermentation. More specifically, hay enhanced the rumen pH and changed the rumen fermentation type. Hay fed calves spent more time on rumination but less time performing abnormal behaviors compared to control. As it can be concluded, feeding oat hay to calves enhances the growth performance, rumen fermentation, and normal calf behaviors, implying improved animal welfare irrespective of the hay length.
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Ermalia, Ayu Afria ulita. "Evaluation Nutritients Of Rice Bran Second Quality Fermented Using Rumen Fluid". Buletin Peternakan 40, nr 2 (30.06.2016): 113. http://dx.doi.org/10.21059/buletinpeternak.v40i2.8700.
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Rice bran is agriculture waste that easy to find. Means to increase biological value of rice bran can do with decrease of highly crude fiber. Treatment that to do with fermentation use rumen fluid from cows. Rumen fluid potential is easy to find in slaughterhouse, this rumen fluid much to never utilization. This purpose of the research for evaluation of rice bran nutrition value that fermentating used rumen fluid, with different levels and long time incubations for get lower crude fiber and higher crude protein content. Methods of this research used Laboratory experiments with Factorial-Random Complete Design 4x4 and 6 repetitions. First factor is rumen fluid levels (0, 30, 40, 50 %/w) and second factor is long incubations (0, 24, 48, 72 hours). This result of ANOVA analysis to show that increment rumen fluid levels and long incubations on rice bran fermentating process can decrease Dry Matter, Organic Matter, Crude Fiber, NDF, ADF and NDR content; as well as increase Crude Protein content but not increase Crude Fat content. The best interaction between rumen fluid levels and long time incubations is 30 %/w with 72 hours long time incubations, with 10.28±0.1 % of Crude Protein, 6.46±0.07 of Crude Fat and 22.31±0.4 of Crude Fiber content.
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Chang, Meinan, Fengtao Ma, Jingya Wei, Junhao Liu, Xuemei Nan i Peng Sun. "Live Bacillus subtilis natto Promotes Rumen Fermentation by Modulating Rumen Microbiota In Vitro". Animals 11, nr 6 (24.05.2021): 1519. http://dx.doi.org/10.3390/ani11061519.
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Previous studies have shown that Bacillus subtilis natto affects rumen fermentation and rumen microbial community structure, which are limited to detect a few microbial abundances using traditional methods. However, the regulation of B. subtilis natto on rumen microorganisms and the mechanisms of microbiota that affect rumen fermentation is still unclear. This study explored the effects of live and autoclaved B. subtilis natto on ruminal microbial composition and diversity in vitro using 16S rRNA gene sequencing and the underlying mechanisms. Rumen fluid was collected, allocated to thirty-six bottles, and divided into three treatments: CTR, blank control group without B. subtilis natto; LBS, CTR with 109 cfu of live B. subtilis natto; and ABS, CTR with 109 cfu of autoclaved B. subtilis natto. The rumen fluid was collected after 0, 6, 12, and 24 h of fermentation, and pH, ammonia nitrogen (NH3-N), microbial protein (MCP), and volatile fatty acids (VFAs) were determined. The diversity and composition of rumen microbiota were assessed by 16S rRNA gene sequencing. The results revealed LBS affected the concentrations of NH3-N, MCP, and VFAs (p < 0.05), especially after 12 h, which might be attributed to changes in 18 genera. Whereas ABS only enhanced pH and NH3-N concentration compared with the CTR group (p < 0.05), which might be associated with changes in six genera. Supplementation with live B. subtilis natto improved ruminal NH3-N and propionate concentrations, indicating that live bacteria were better than autoclaved ones. This study advances our understanding of B. subtilis natto in promoting ruminal fermentation, providing a new perspective for the precise utilization of B. subtilis natto in dairy rations.
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Wang, Weikang, Qichao Wu, Wenjuan Li, Yanlu Wang, Fan Zhang, Liangkang Lv, Shengli Li i Hongjian Yang. "High-Gossypol Whole Cottonseed Exhibited Mediocre Rumen Degradability and Less Microbial Fermentation Efficiency than Cottonseed Hull and Cottonseed Meal with an In Vitro Gas Production Technique". Fermentation 8, nr 3 (28.02.2022): 103. http://dx.doi.org/10.3390/fermentation8030103.
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To explore whether or not the gossypol varied in cottonseed by-products affect rumen degradability and fermentation efficiency, an in vitro cumulative gas production experiment was applied with mixed rumen microorganism to compare rumen fermentation characteristics of whole cottonseed (WCS, n = 3 samples), cottonseed meal (CSM, n = 3 samples), and cottonseed hull (CSH, n = 2 samples). The five-replicate fermentation per sample per incubation time continuously lasted for 0.5, 1.5, 3, 6, 12, 24, 36, and 48 h with an automated gas production recording system. Regardless of distinct nutrient differences, the free gossypol level in these cottonseed by-products ranked: WCS > CSH > CSM. After 48 h of incubation, the in vitro dry matter degradability and ammonia N concentration ranked as: CSM > WCS > CSH. The cumulative gas production and total volatile fatty acid (VFA) levels in the culture fluids ranked: CSM > CSH > WCS, in which the average production rate ranked: CSM > WCS > CSH. Regarding the molar VFA pattern, WCS in comparison with CSH and CSM presented the lowest production of non-glucogenic acids (e.g., acetate) and exhibited the highest fermentation efficiency of energy from carbohydrates to VFAs. There was a significant negative correlation between the gossypol content and cumulative gas and total VFA production, suggesting that the greater gossypol in cottonseed by-products, the more detrimental effect occurred for rumen fermentation. In a brief, WCS exhibited mediocre rumen degradability and less microbial fermentation efficiency than CSH and CSM, depending on their gossypol levels.
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Wang, Haibo, Fei Wu, Tianci Guan, Yangxiang Zhu, Zhantao Yu, Depeng Zhang, Siyu Zhang, Huawei Su i Binghai Cao. "Chopping Roughage Length Improved Rumen Development of Weaned Calves as Revealed by Rumen Fermentation and Bacterial Community". Animals 10, nr 11 (19.11.2020): 2149. http://dx.doi.org/10.3390/ani10112149.
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Roughage particle size can influence rumen development, which is also determined by rumen microorganisms and their metabolic end-products. Therefore, the aim of this study was to evaluate the comprehensive effects of roughage length and rumen bacterial community on the rumen development of weaned calves. A total of thirty-six weaned Angus female calves (125 ± 3 d; 161.2 ± 13.0 kg) were randomly assigned to three diets differing in roughage particle size: 4 cm (short length); 24 cm (medium length); and 44 cm (long length). Results showed that chopping roughage increased dry matter intake and organic matter apparent digestibility; altered rumen fermentation indicated by the increased rumen butyrate and valerate concentrations; and increased plasma glucose, cholesterol, and total protein. Chopping roughage affected rumen bacterial community, as indicated by altering the diversity indices; by increasing ruminal bacteria Papillibacter and Eubacterium_hallii_group, which are involved in butyrate production; and by increasing Synergistetes and Mogibacterium, which are involved in bacterial colonization. In conclusion, chopping roughage at 4 cm was shown to improve the rumen bacterial community, alter rumen fermentation, eventually promote the development of rumen.