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1
MacLaren, Robert E. "Optic nerve regeneration". Thesis, University of Oxford, 1995. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.318923.
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2
Brecknell, John Edward. "The rat nigrostriatal system : regeneration and reconstruction". Thesis, University of Cambridge, 1995. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.262821.
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3
Painter, Michio Wendell. "Regeneration in the aging peripheral nervous system". Thesis, Harvard University, 2014. http://dissertations.umi.com/gsas.harvard:11422.
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In the peripheral nervous system (PNS), aging is associated with a number of disorders, including a decline in regenerative capacity after injury. Although this decline has been observed in both rodents and humans for decades, the cellular and molecular underpinnings of this defect have remained elusive. As such, the goal of this thesis was to elucidate, at least in part, how aging impinges on axonal regeneration.
4
Hager, Elizabeth A. (Elizabeth Ann). "Composite gelatin delivery system for bone regeneration". Thesis, Massachusetts Institute of Technology, 2004. http://hdl.handle.net/1721.1/32844.
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Thesis (S.B.)--Massachusetts Institute of Technology, Dept. of Materials Science and Engineering, June 2005.Includes bibliographical references (p. 38-39).
In this thesis, the chemical/mechanical properties and biocompatibility of gelatin were investigated to produce a gelatin scaffold for the release of bone morphogenetic proteins (BMPs) from composite particles. This delivery system, designed to regenerate bone, holds much promise as an alternative to bone grafts. The chemical properties of gelatin were examined through zeta potential measurements, swelling studies, optical microscopy, environmental scanning electron microscopy (ESEM), and collagenase degradation. Compressive tests and mercury porosimetry were performed to study the mechanical and structural properties of the scaffold. The biocompatibility of the scaffold was determined through cell optical imaging and DNA quantification studies. Based on findings of this research, the material choices were made and the synthesis method for the gelatin scaffold was developed. Gelatin A, 300B, derived from bovine collagen, with an isoelectric point of [approx.] 9, was selected. Crosslinking was accomplished by reacting 10 w/v% glutaraldehyde with 10 w/v% gelatin solution. The most effective crosslinking condition was found to be 5 hours at room temperature. Glycine rinses were conducted to cap any non- reacted (toxic) aldehyde groups, and the necessary length of time was found to be at least 48 hours at 37⁰C. Finally, based on pore size distribution and mechanical stability, an optimal lyophilization method was developed with initial freezing at -20⁰C for 1 day, followed by lyophilization of the scaffold for 1-2 days. In terms of mechanical properties of the gelatin and amount of protein delivered, the most effective loading of poly(lactic-co-glycolic acid)/apatite/protein composite particles was found to be 10% of the mass of the gelatin.
by Elizabeth A. Hager.
S.B.
5
Stumpf, da Silva Taisa Regina. "Delivery Systems to Enhance Neural Regeneration in the Central Nervous System". Thesis, Université d'Ottawa / University of Ottawa, 2019. http://hdl.handle.net/10393/39391.
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6
Hu, Ying. "Optic nerve regeneration in adult rat /". Connect to this title, 2006. http://theses.library.uwa.edu.au/adt-WU2007.0080.
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7
Cheah, Menghon. "Integrin activation in axon regeneration". Thesis, University of Cambridge, 2015. https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.708902.
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8
Vidal, Iglesias Berta. "The fibrinolitys system in muscle regeneration and dystrophy". Doctoral thesis, Universitat Pompeu Fabra, 2008. http://hdl.handle.net/10803/7143.
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Duchenne muscular dystrophy (DMD) is a fatal degenerative disorder of locomotor and respiratory muscles, in which myofibers are progressively replaced by non-muscular fibrotic tissue. Here, we show that fibrin/ogen accumulates in dystrophic muscles of DMD patients and of the mdx mouse model of DMD. Genetic loss or pharmacological depletion of fibrin/ogen in mdx mice attenuated muscular dystrophy progression and improved locomotor capacity. More importantly, fibrin/ogen depletion reduced fibrosis in mdx mouse diaphragm. Our data indicate that fibrin/ogen, through induction of IL-1 Ò, drives the synthesis of TGF Ò by mdx macrophages, which in turn, induces collagen production in mdx fibroblasts. Fibrin/ogen-produced TGF Ò further amplifies collagen accumulation through recruitment and activation of pro-fibrotic alternatively activated macrophages. Fibrin/ogen also stimulated collagen synthesis directly in mdx fibroblasts, via Ñv Ò3 integrin engagement. In addition, when analyzing a group of 39 DMD patients, fibrin/ogen accumulation in locomotor muscles was found associated with fibrosis and disease severity. These data unveil a novel role of fibrin/ogen in muscular dystrophy and, importantly, in the replacement of muscle by fibrotic tissue.
9
Lapan, Sylvain William. "Regeneration and maintenance of the planarian nervous system". Thesis, Massachusetts Institute of Technology, 2012. http://hdl.handle.net/1721.1/87912.
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Thesis: Ph. D., Massachusetts Institute of Technology, Department of Biology, 2012.Cataloged from PDF version of thesis.
Includes bibliographical references
Planarians can regenerate all tissues, including the central nervous system and the eyes. This process depends on a population of cells in the adult, the neoblasts, that includes pluripotent stem cells. Whether the neoblast population also includes progenitors specialized for forming specific lineages has not been demonstrated. This thesis describes the identification of progenitors that contribute to eyes during regeneration. Expression and functional analyses identified the genes eyes absent, six- 1/2 and ovo as critical for the formation of all cells of the eye. otxA and soxB were specifically required for photoreceptor regeneration, and sp6-9 and dlx were required for regeneration of the optic pigment cup. Expression analysis of these transcription factors in situ revealed that eye progenitors were distributed in mesenchymal trails extending posteriorly from the regenerating eye. These progenitors originate in the neoblasts, and promixity to the eye primordium correlates with increased differentiation. The spatial and genetic identification of a progenitor population in planarians elucidates migratory and morphogenetic mechanisms underlying organ regeneration in these animals. RNA sequencing of eye tissue also identified hundreds of genes with highly enriched expression in the eye, including numerous orthologs of eye pathology-related gene as well as likely components of key visual processes such as phototransduction and optic pigment cell function. The planarian brain is composed of dozens of cell types with regionalized distribution. The function of the planarian hedgehog gene in the patterning of CNS regions was investigated. hedgehog was expressed in the medial planarian brain, flanked by nkx2 and nkx6, then pax6b, and finally dlx-1 and msx at the most distal positions. This organization is similar to the expression domains of orthologous transcription factors in the vertebrate neural tube. However, in contrast to vertebrates, the expression of nkx2, nkx6, and pax6b in planarians was not affected by loss of hedgehog expression. RNA sequencing analysis identified a strong effect of Hedgehog signaling genes on a medially positioned cell with glia-like features. Therefore, Hedgehog signaling affects formation of at least one cell type in the planarian brain, but does not broadly regulate transcription factor expression domains and cell type identity.
by Sylvain William Lapan.
Ph. D.
10
Pellegrino, Cristina. "Decision support system for Brownfield Site Sustainable Regeneration". Thesis, Cardiff University, 2007. http://orca.cf.ac.uk/54600/.
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The work described in this thesis concerns the development of a Brownfield Site Sustainable Regeneration - Decision Support System (BSSR-DSS). The project aims to develop a pilot system that can provide information and alternatives for sustainable brownfield regeneration as a platform to make decisions in this context. One of the main characteristics of the BSSR-DSS is its ability to process the input data (related to site characteristics), run simulations and assess/evaluate different scenarios in order to obtain the most sustainable solution. The process input data for the BSSR-DSS relates to a wide range of sustainability indicators that have been developed in the European Project "RESCUE - Regeneration of European Sites in Cities and Urban Environments". The system involves the elaboration of methods and approaches using innovative mathematical techniques such as Artificial Neural Networks and Fuzzy Logic to analyze and evaluate the input data (site characteristics and sustainability indicators) to produce a significant output. The literature review undertaken confirmed that no current system integrating the two above mathematical techniques has been implemented to date. The system is also linked to a Geographic Information System (GIS) called MAPINFO. This allows extensive information searches to be undertaken that can be site specific, and the information displayed on a map.
11
Hawthorne, Alicia Lynn. "The Development and Regeneration of the Serotonergic System". Case Western Reserve University School of Graduate Studies / OhioLINK, 2010. http://rave.ohiolink.edu/etdc/view?acc_num=case1264027000.
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12
Pamphlett, Roger Stephen. "The role of the axon and of the nerve cell body in axonal regeneration". Doctoral thesis, University of Cape Town, 1989. http://hdl.handle.net/11427/27181.
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13
Hu, Ying. "Optic nerve regeneration in adult rat". University of Western Australia. School of Anatomy and Human Biology, 2007. http://theses.library.uwa.edu.au/adt-WU2007.0080.
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[Truncated abstract] There is limited intrinsic potential for repair in the adult human central nervous system (CNS). Dysfunction resulting from CNS injury is persistent and requires prolonged medical treatment and rehabilitation. The retina and optic nerve are CNSderived, and adult retinal ganglion cells (RGCs) and their axons are often used as a model in which to study the mechanisms associated with injury, neuroprotection and regeneration. In this study I investigated the effects of a variety of strategies on promoting RGC survival and axonal regeneration after optic nerve injury, including the use of reconstructed chimeric peripheral nerve (PN) grafts, gene therapy, and intraocular application of pharmacological agents and other factors . . . C3 transferase is an enzyme derived from Clostridium botulinum that inactivates Rho GTPase. Because SC myelin contains MAG and PN also contains CSPGs, I tested the effects of intraocular injection of a modified form of C3 (C3-11), provided by Dr Lisa McKerracher (CONFIDENTIAL data, under IP agreement with Bioaxone Therapeutic, Montreal) on RGC axonal regeneration into PN autografts. My results showed that there was significantly more RGC survival and axonal regeneration in PN autografts after repeated intraocular injection of C3. I also tested whether intraocular injections of CPT-cAMP and/or CNTF can act in concert with the C3 to further increase RGC survival and/or regeneration. Results showed that the effect of C3 and CPT-cAMP plus CNTF were synergistic and partially additive. The use of combination therapies therefore offers the best hope for robust and substantial regeneration. The overall results from my PhD project will help determine how best to reconstruct nerve pathways and use pharmacological interventions in the clinical treatment of CNS injury, hopefully leading to improved functional outcomes after neurotrauma.
14
Toma, Jeremy Steven. "Immunohistochemical analyses of nervous system structure, development and regeneration". Thesis, University of British Columbia, 2006. http://hdl.handle.net/2429/31284.
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Specific aspects of nervous system structure, development, and regeneration were
investigated in two separate studies. The first study was concerned with development of
sensory root entry zones. Sensory information enters the central nervous system (CNS)
via root entry zones where sensory axons span a glial environment consisting of Schwann
cells in the peripheral nervous system (PNS) and astrocytes and oligodendrocytes in the
CNS. Little is known about the postnatal development of the glial elements of many root
entry zones. I sought to establish a comparative developmental timecourse of the glial
elements in the postnatal (PO, P3, P7, P14) and adult rat of three root entry zones: the
spinal nerve dorsal root entry zone, the trigeminal root entry zone, and the vagal dorsal
root entry zone. I compared entry zone development based on the expression of antigens
in peripheral glia, central glia, and the PNS extracellular matrix. While all three root
entry zones had reached maturity by PI4, the glial elements comprising the PNS-CNS
interface of the trigeminal root entry zone and the vagal dorsal root entry zone matured
earlier than those of the spinal nerve dorsal root entry zone.
This study revealed unexpected expression patterns of certain glial antigens. For
example, the antibody used to label mature oligodendrocytes (RIP) labelled Schwann cell
cytoplasm. I sought to follow up on this observation and characterized RTP
immunoreactivity in peripheral glia in the second study. In uninjured rats, RIP
demarcated paranodal regions of myelinated axons and clearly defined Schmidt-
Lantermann incisures. Robust RIP immunoreactivity was present in Remak bundles.
Low levels of RIP immunoreactivity were detectable in satellite cells surrounding dorsal
root ganglion (DRG) neurons and in terminal Schwann cells at neuromuscular junctions.
These results suggested a correlation between RIP immunoreactivity and amount of
axoglial contact. Injury induced sympathetic sprouting and pericellular basket formation
in the DRG was conducted to further examine this correlation. All perineuronal
sympathetic sprouts infiltrated heavily RlP-immunoreactive satellite cell sheaths. RIP
immunoreactivity was absent from placodal-derived olfactory ensheathing cells,
suggesting that correlation between axoglial contact and RIP immunoreactivity is
confined to peripheral glia of neural crest origin.Science, Faculty of
Zoology, Department of
Graduate
15
Bampton, Edward Thomas William. "Factors secreted by Schwann cells in nervous system regeneration". Thesis, University of Oxford, 2001. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.249635.
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16
Hawkins, Sara Joy. "The timing of regeneration in the amphibian olfactory system". Master's thesis, Universidade de Aveiro, 2015. http://hdl.handle.net/10773/15444.
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Mestrado em Biologia Molecular e CelularComprehending the mechanisms that make lifelong neurogenesis possible has a clear interest for the better understanding of the basic principles that govern cellular and molecular interactions in the nervous system, as well as a relevant clinical interest. The limited ability of the central nervous system to generate new neurons in order to replace those that have been lost is a formidable obstacle to recovery from neuronal damage caused by injury or neurodegenerative disease. The olfactory system (OS) is an ideal system to study the process of neuronal recovery after injury, as it is known for its lifelong capacity to replenish cells lost during natural turnover, as well as its remarkable ability to regenerate after severe lesion. The olfactory epithelium (OE) shows neurogenesis throughout life. Newly differentiated olfactory receptor neurons (ORNs) are continuously reintegrated into an existing circuitry to maintain the sense of smell. The aim of this thesis is to describe the morphological and functional alterations that occur over time in the OS of larval Xenopus laevis, after transection of the olfactory nerve (ON). Results obtained using immunohistochemistry essays, as well as sensory neuron labeling and calcium imaging techniques, indicate that ORN cell death reaches its peak 48 hours after transection, and that proliferating stem cells found in the basal cell layer of the OE are quickly upregulated after lesion. Supporting cells seem to maintain both morphological and functional integrity after transection of the ON. The OE recovers its original morphological structure 1 week after transection, at which time the first axons reach the olfactory bulb (OB) and begin the process of reinnervation. Spontaneous activity of mitral/tufted cells occurs in the OB during the first weeks after transection but no odor-induced activity is observed. After 3-4 weeks glomerular responses were observed in some animals upon application of stimulus, but the response and glomerular morphology are clearly altered as compared to control. After 6-7 weeks responses seem to have fully recovered, indicating that the OS of larval X. laevis recovers morphologically and functionally 6-7 weeks after ON transection.
O estudo dos mecanismos responsáveis pela neuro-regeneração tem um marcado interesse para a compreensão dos princípios básicos que governam as interações celulares e moleculares no sistema nervoso, bem como um interesse clínico relevante. A limitada capacidade do sistema nervoso central para dar origem a novos neurónios é um obstáculo formidável para a recuperação do sistema após lesão neuronal ou doença neurodegenerativa. O sistema olfativo é um sistema ideal para o estudo do processo de recuperação após lesão neuronal, pois é conhecido no mundo científico pela sua capacidade contínua e vitalícia para repor células perdidas durante a renovação celular natural, bem como a sua notável capacidade para regenerar após uma lesão grave. O epitélio olfativo apresenta a capacidade para dar origem a novos neurónios ao longo de toda a vida. Neurónios sensoriais olfativos diferenciados são continuamente reintegrados num circuito já existente, mantendo assim o sentido do olfato. O objetivo desta tese é descrever as alterações morfológicas e funcionais que ocorrem ao longo do tempo no sistema olfativo de Xenopus laevis em estado larvar, após o corte do nervo olfativo. Os resultados obtidos através do uso de ensaios de imunohistoquímica, bem como técnicas de marcação neuronal sensorial e de imagiologia de cálcio, indicam que a morte celular na população de neurónios sensoriais olfativos atinge o seu máximo 48 horas após a lesão, e que células estaminais encontradas na camada basal do epitélio olfativo são positivamente reguladas após lesão e proliferam rapidamente. Células de suporte parecem manter tanto a integridade morfológica como funcional após o corte do nervo olfativo. O epitélio olfativo recupera a sua estrutura morfológica inicial 1 semana após a lesão, momento em que os primeiros axónios atingem o bolbo olfativo e começam o processo de reintegração. Ocorre atividade espontânea das células mitrais/tufados do bolbo olfativo durante as primeiras semanas após a lesão, mas nenhuma atividade induzida por estímulo com odor foi observada. Depois de 3-4 semanas, atividade glomerular foi observada em alguns animais após a aplicação de estímulos, mas a resposta e morfologia glomerular foram claramente alteradas em relação ao controlo. Depois de 6-7 semanas as respostas parecem ter recuperado totalmente, indicando que o sistema olfativo de X. laevis em estado larvar recupera morfológica e funcionalmente 6-7 semanas após o corte do nervo olfativo.
17
Bozza, Angela. "Alginate-based Hydrogels for Central Nervous System Tissue Regeneration". Doctoral thesis, Università degli studi di Trento, 2015. https://hdl.handle.net/11572/368725.
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As the central nervous system shows very little capability for self-repair following injury, regenerative medicine approaches are increasingly interested in the use of stem cells for cell replacement strategies. Biomaterials are an interesting tool to carry out this type of therapies. They allow three-dimensional cultures for stem cells differentiation and are helpful in order to obtain cells at the right developmental stage for transplantation. Moreover, they could help to enhance and control cell survival after transplantation, minimizing cell death. Stroke is a very severe form of brain injury and one of the leading causes of death worldwide, as no effective cures are available. Several studies show that neural stem cells (NSCs) are able to integrate and improve functional recovery once transplanted in stroke animal models. However, the majority of the grafted NSCs die within weeks after transplantation, limiting treatment efficacy. Tissue engineering approaches aim to restore tissue functions combining principles of cell biology and engineering, using designed and tailored three-dimensional biomaterial scaffolds. In this study we tested alginate as candidate biomaterial for neural tissue repair. We studied its ability to support mouse embryonic stem cells (mESCs) neural differentiation in vitro. We evaluated whether changes in its concentration or modifications with extracellular matrix components could influence cell differentiation, analysing the mechanical and physical properties of the generated scaffolds. In the first part, we evaluated the suitability of alginate as a scaffold for three-dimensional cultures able to enhance differentiation of mESCs towards neural lineages. We tested whether encapsulation of mESCs within alginate beads could support and/or enhance neural differentiation with respect to two-dimensional cultures. We encapsulated cells in beads of alginate at two different concentrations, with or without modification by fibronectin, RGD peptide or hyaluronic acid. Cells survive and differentiate inside our scaffolds, forming clusters. Gene expression analyses showed that cells grown in alginate scaffolds increase differentiation toward neural lineages with respect to the two dimensional controls. Immunocytochemistry analyses confirmed these results, further showing terminal differentiation of neurons by the expression of synaptic markers. Cells showed also the capability to form networks among themselves and with cells of other clusters. All the scaffolds we prepared resembled brain tissue characteristics, thus we decided to test alginate as potential support for tissue engineering approaches in the injured brain. In the second part of the work we tested alginate as support for NSCs injection in the brain. We evaluated in vivo crosslinking of alginate after injection, and verified inflammation levels due to its presence in mouse brain tissue. Our preliminary studies suggest that alginate polymerizes in vivo, forming a hydrogel, and that it does not elicit any inflammatory response following injection. Our data show that alginate, alone or modified, is a suitable biomaterial to promote in vitro differentiation of pluripotent cells toward neural fates. Moreover, it could be used as injectable hydrogel for brain tissue regeneration. We plan to co-inject alginate with NSCs in stroke mouse models in order to enhance viability and integration of the engrafted cells in the damaged tissue. We plan to study alginate permanence in the brain and NSCs viability, integration and capability to stimulate regeneration after ischemic injury.
18
Bozza, Angela. "Alginate-based Hydrogels for Central Nervous System Tissue Regeneration". Doctoral thesis, University of Trento, 2015. http://eprints-phd.biblio.unitn.it/1546/1/BozzaAngela_PhDThesis_FINAL.pdf.
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As the central nervous system shows very little capability for self-repair following injury, regenerative medicine approaches are increasingly interested in the use of stem cells for cell replacement strategies. Biomaterials are an interesting tool to carry out this type of therapies. They allow three-dimensional cultures for stem cells differentiation and are helpful in order to obtain cells at the right developmental stage for transplantation. Moreover, they could help to enhance and control cell survival after transplantation, minimizing cell death. Stroke is a very severe form of brain injury and one of the leading causes of death worldwide, as no effective cures are available. Several studies show that neural stem cells (NSCs) are able to integrate and improve functional recovery once transplanted in stroke animal models. However, the majority of the grafted NSCs die within weeks after transplantation, limiting treatment efficacy. Tissue engineering approaches aim to restore tissue functions combining principles of cell biology and engineering, using designed and tailored three-dimensional biomaterial scaffolds. In this study we tested alginate as candidate biomaterial for neural tissue repair. We studied its ability to support mouse embryonic stem cells (mESCs) neural differentiation in vitro. We evaluated whether changes in its concentration or modifications with extracellular matrix components could influence cell differentiation, analysing the mechanical and physical properties of the generated scaffolds. In the first part, we evaluated the suitability of alginate as a scaffold for three-dimensional cultures able to enhance differentiation of mESCs towards neural lineages. We tested whether encapsulation of mESCs within alginate beads could support and/or enhance neural differentiation with respect to two-dimensional cultures. We encapsulated cells in beads of alginate at two different concentrations, with or without modification by fibronectin, RGD peptide or hyaluronic acid. Cells survive and differentiate inside our scaffolds, forming clusters. Gene expression analyses showed that cells grown in alginate scaffolds increase differentiation toward neural lineages with respect to the two dimensional controls. Immunocytochemistry analyses confirmed these results, further showing terminal differentiation of neurons by the expression of synaptic markers. Cells showed also the capability to form networks among themselves and with cells of other clusters. All the scaffolds we prepared resembled brain tissue characteristics, thus we decided to test alginate as potential support for tissue engineering approaches in the injured brain. In the second part of the work we tested alginate as support for NSCs injection in the brain. We evaluated in vivo crosslinking of alginate after injection, and verified inflammation levels due to its presence in mouse brain tissue. Our preliminary studies suggest that alginate polymerizes in vivo, forming a hydrogel, and that it does not elicit any inflammatory response following injection. Our data show that alginate, alone or modified, is a suitable biomaterial to promote in vitro differentiation of pluripotent cells toward neural fates. Moreover, it could be used as injectable hydrogel for brain tissue regeneration. We plan to co-inject alginate with NSCs in stroke mouse models in order to enhance viability and integration of the engrafted cells in the damaged tissue. We plan to study alginate permanence in the brain and NSCs viability, integration and capability to stimulate regeneration after ischemic injury.
19
Uhrig, Brent A. "Tissue regeneration in composite injury models of limb trauma". Diss., Georgia Institute of Technology, 2013. http://hdl.handle.net/1853/49080.
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Severe extremity trauma often involves significant damage to multiple tissue types, including bones, skeletal muscles, peripheral nerves, and blood vessels. Such injuries present unique challenges for reconstruction, and improving structural and functional outcomes of intervention remains a pressing, unmet clinical need. While tissue engineering/regenerative medicine (TE/RM) therapeutics offer promising potential to overcome the status quo limitations of surgical reconstruction, very few products have transitioned to clinical practice. Improving treatment options will likely require advancing our understanding of the biological interactions occurring in the repair of damaged tissues.
Bone tissue is known to be innervated and highly vascularized, and both tissue types are involved in normal bone physiology. However, the degree to which these tissue relationships influence the repair of large, multi-tissue defects remains unknown. Accordingly, the goal of this thesis was to investigate tissue regeneration in two novel composite injury models. First, we characterized interactions in a composite bone and nerve injury model where a segmental bone defect was combined with a peripheral nerve gap. Our results indicated that although tissue regeneration was not impaired, the composite injury group experienced a marked functional deficit in the operated limb compared to single-tissue injury. Second, we developed a model of composite bone and vascular extremity trauma by combining a critically-sized segmental bone defect with surgically-induced hind limb ischemia to evaluate the effects on BMP-2-mediated bone repair. Interestingly, our results demonstrated a stimulatory effect of the recovery response to ischemia on bone regeneration. Finally, we investigated early vascular growth and gene expression as potential mechanisms coupling the response to ischemia with bone defect repair. Although the response to ischemia promoted robust vascular growth in the thigh, it did not directly augment vascularization at the site of bone regeneration. In addition, the stimulatory effects of ischemia on bone regeneration could not be explained by gene expression alone based on the genes and time points investigated.
Taken together, this thesis presents pioneering work on a new thrust of TE/RM research – tissue regeneration in models of composite injury. This work has provided new insights on the complexity of composite tissue repair, specifically in regard to the relationship between vascular tissue growth and bone healing. Going forward, successful leverage of models of composite tissue injuries will provide valuable test beds for screening new technologies, advance the understanding of tissue repair biology, and ultimately, may produce new therapeutic interventions for limb salvage and reconstruction that improve outcomes for extremity trauma patients.
20
Vujic, Nikola. "Power Regeneration in Actively Controlled Structures". Thesis, Virginia Tech, 2002. http://hdl.handle.net/10919/33425.
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The power requirements imposed on an active vibration isolation system are quite important to the overall system design. In order to improve the efficiency of an active isolation system we analyze different feedback control strategies which will provide electrical energy regeneration. The active isolation system is modeled in a state-space form for two different types of actuators: a piezoelectric stack actuator and a linear electromagnetic (EM) actuator. During regenerative operation, the power is flowing from the mechanical disturbance through the electromechanical actuator and its switching drive into the electrical storage device (batteries or capacitors). We demonstrate that regeneration occurs when controlling one or both of the flow states (velocity and/or current). This regenerative control strategy affects the closed loop dynamics of the isolator which sees its damping reduced.Master of Science
21
Sousa, Filipe Pinto Teixeira. "Development and functional regeneration of the zebrafish lateral line system". Doctoral thesis, Universitat Pompeu Fabra, 2012. http://hdl.handle.net/10803/78873.
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Per a aquesta tesi, utilitzo la línia lateral del peix zebra com a sistema model
per adreçar dues qüestions fonamentals:
En una primera línia d’investigació exploro la relació entre la funció d’un
òrgan i la seva arquitectura. La regeneració de cèl.lules ciliades a la línia
lateral del peix zebra ocorre mitjançant la divisió dels seus progenitors en
determinades posicions dins les zones ventral i dorsal del neuromast. Durant
la regeneració de les cèl.lules ciliades, es forma una línia de simetria vertical,
que bisecciona l’epiteli del neuromast en dues meitats de polaritats planars
oposades. La qüestió de com es controla l’anisotropia de la regeneració de les
cèl.lules ciliades i com integrar aquest procés en l’establiment de la simetria
bilateral d’aquest organ, roman encara per esclarir. En aquest estudi mostro
que la simetria bilateral del neuromast es sosté degut a l’activitat
compartimentalitzada de Notch qui, permetent l’estabilització dels
progenitors de cèl.lules ciliades en compartiments polars específics, organitza
l’anisotropia de la regeneració.
En una segona línia d’investigació, descric el rol del complex de remodelació
de cromatina ATPasa brg1 durant la formació d’organs mecanosensorials al
peix zebra. Així mostro que els mutants de brg1 desenvolupen un sistema de
linia lateral truncat, donat que brg1 es necessari per a la regulació de múltiples
events cel.lulars al primordi de la línia lateral.In this thesis I use the zebrafish lateral line as a model system to address two fundamental questions. In a first line of investigation I explore the relation between an organ function and its architecture. The regeneration of hair cells in the zebrafish lateral line occurs trough the division of hair-cell progenitors at specific locations in the dorsal and ventral aspects of the neuromasts. As hair cells regenerate a vertical midline that bisects the neuromast epithelium into perfect mirror-symmetric plane-polarized halves is formed. Each half contains hair cells of identical planar orientation but opposite to that of the confronting half. How hair cell regeneration anisotropy is controlled and how this process is integrated in the establishment of this organ bilateral symmetry is poorly understood. Here I show that the neuromast bilateral symmetry is sustained by compartmentalized Notch activity, which governs regeneration anisotropy by permitting the stabilization of hair cell progenitors in specific polar compartments. In a second line of research I report the role of the chromatin remodeling complex ATPase brg1 during mechanosensory organ formation in the zebrafish. I show that brg1 mutants develop a truncated lateral line system as brg1 is needed in the regulation of multiple cellular events in the lateral line primordium.
22
Verrall, Jason. "Studies on the regeneration of the leech central nervous system". Thesis, University of Oxford, 2002. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.270723.
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Sandvig, Axel. "Investigation of axon regeneration in the higher vertebrate nervous system". Thesis, University of Cambridge, 2000. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.621808.
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Naidu, Murali D. Kuppusamy. "Environmental influences and axonal mechanisms in peripheral nervous system regeneration". Thesis, University of Cambridge, 2003. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.620007.
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Lunn, Elizabeth Ruth. "Studies on the degeneration and regeneration of neurons to skeletal muscle". Thesis, University of Oxford, 1989. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.292675.
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Markert, Chad D. "Ultrasound and exercise in skeletal muscle regeneration". Connect to this title online, 2004. http://rave.ohiolink.edu/etdc/view?acc%5Fnum=osu1091304498.
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Thesis (Ph. D.)--Ohio State University, 2004.Document formatted into pages. Includes bibliographical references. Abstract available online via OhioLINK's ETD Center; full text release delayed at author's request until 2005 Aug. 2.
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Vázquez-Figueroa, Eduardo. "Development of a novel dehydrogenase and a stable cofactor regeneration system". Atlanta, Ga. : Georgia Institute of Technology, 2008. http://hdl.handle.net/1853/31685.
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Thesis (Ph.D)--Chemical Engineering, Georgia Institute of Technology, 2009.Committee Chair: Bommarius, Andreas S.; Committee Member: Doyle, Donald F.; Committee Member: Koros, William J.; Committee Member: Moore, Jeffre C.; Committee Member: Prausnitz, Mark R. Part of the SMARTech Electronic Thesis and Dissertation Collection.
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Vázquez-Figueroa, Eduardo. "Development of a novel dehydrogenase and a stable cofactor regeneration system". Diss., Georgia Institute of Technology, 2008. http://hdl.handle.net/1853/31685.
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The first goal of this work focused on the development of an amine dehydrogenase (AmDH) from a leucine dehydrogenase using site-directed mutagenesis. We aimed at reductively aminating a prochiral ketone to a chiral amine by using leucine dehydrogenase (LeuDH) as a starting template. This initial work was divided into two stages. The first focused mutagenesis to a specific residue (K68) that we know is key to developing the target functionality. Subsequently, mutagenesis focused on residues known to be in close proximity to a key region of the substrate (M65 and K68). This approach allowed for reduced library size while at the same time increased chances of generating alternate substrate specificity. An NAD+-dependent high throughput assay was optimized and will be discussed. The best variants showed specific activity in mU/mg range towards deaminating the target substrate.
The second goal of this work was the development of a thermostable glucose dehydrogenase (GDH) starting with the wild-type gene from Bacillus subtilis. GDH is able to carry out the regeneration of both NADH and NADPH cofactors using glucose as a substrate. We applied the structure-guided consensus method to identify 24 mutations that were introduced using overlap extension. 11 of the tested variants had increased thermal stability, and when combined a GDH variant with a half-life ~3.5 days at 65℃ was generated--a ~10⁶increase in stability when compared to the wild-type.
The final goal of this work was the characterization of GDH in homogeneous organic-aqueous solvent systems and salt solutions. Engineered GDH variants showed increased stability in all salts and organic solvents tested. Thermal stability had a positive correlation with organic solvent and salt stability. This allowed the demonstration that consensus-based methods can be used towards engineering enzyme stability in uncommon media. This is of significant value since protein deactivation in salts and organic solvents is not well understood, making a priori design of protein stability in these environments difficult.
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Yusuf, Syed Adnan. "An evolutionary AI-based decision support system for urban regeneration planning". Thesis, University of Wolverhampton, 2010. http://hdl.handle.net/2436/114896.
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The renewal of derelict inner-city urban districts suffering from high levels of socio-economic deprivation and sustainability problems is one of the key research areas in urban planning and regeneration. Subject to a wide range of social, economical and environmental factors, decision support for an optimal allocation of residential and service lots within such districts is regarded as a complex task. Pre-assessment of various neighbourhood factors before the commencement of actual location allocation of various public services is considered paramount to the sutainable outcome of regeneration projects. Spatial assessment in such derelict built-up areas requires planning of lot assignment for residential buildings in a way to maximize accessibility to public services while minimizing the deprivation of built neighbourhood areas. However, the prediction of socio-economic deprivation impact on the regeneration districts in order to optimize the location-allocation of public service infrastructure is a complex task. This is generally due to the highly conflicting nature of various service structures with various socio-economic and environmental factors. In regards to the problem given above, this thesis presents the development of an evolutionary AI-based decision support systemto assist planners with the assessment and optimization of regeneration districts. The work develops an Adaptive Network Based Fuzzy Inference System (ANFIS) based module to assess neighbourhood districts for various deprivation factors. Additionally an evolutionary genetic algorithms based solution is implemented to optimize various urban regeneration layouts based upon the prior deprivation assessment model. The two-tiered framework initially assesses socio-cultural deprivation levels of employment, health, crime and transport accessibility in neighbourhood areas and produces a deprivation impact matrix overthe regeneration layout lots based upon a trained, network-based fuzzy inference system. Based upon this impact matrix a genetic algorithm is developed to optimize the placement of various public services (shopping malls, primary schools, GPs and post offices) in a way that maximize the accessibility of all services to regenerated residential units as well as contribute to minimize the measure of deprivation of surrounding neighbourhood areas. The outcome of this research is evaluated over two real-world case studies presenting highly coherent results. The work ultimately produces a smart urban regeneration toolkit which provides designer and planner decision support in the form of a simulation toolkit.
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Proctor, John William. "Investigation and development of the diesel particulate filter autoselective regeneration system". Thesis, Loughborough University, 2006. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.547385.
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Sengottuvel, Vetrivel [Verfasser]. "Microtubule stabilization facilitates axon regeneration in central nervous system / Vetrivel Sengottuvel". Ulm : Universität Ulm. Medizinische Fakultät, 2012. http://d-nb.info/1024126919/34.
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Lin, Rachel. "The role of proteoglycans in regeneration in the central nervous system". Thesis, University of Cambridge, 2007. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.613245.
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Gaspar, Diana Patrícia Rodrigues. "Novel strategy to produce a drug delivery system for skin regeneration". Master's thesis, Universidade da Beira Interior, 2012. http://hdl.handle.net/10400.6/1118.
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Skin lesions are traumatic events that lead to the increase of fluid loss, infections, scarring and locally immunocompromised regions. These injuries can be caused by genetic disorders, acute trauma or even surgical interventions. In these situations, a substantial area of skin can be damaged, often without the possibility of being regenerated. Scientists have put a lot of effort in the development of suitable drug delivery systems suitable to release therapeutic molecules that are required for the initials phases of the wound healing process. Cell microencapsulation arises as an alternative approach for sustained in situ cell delivery. This technology is based on the immobilization of cells within a polymeric matrix, surrounded by a semi-permeable membrane, that isolate the encapsulated cells from the host immune system. Nonstanding, the microparticulate matrix still allows the exchange of nutrients, gases, waste and releasing of bioactive molecules, such as extracellular matrix components and growth factors secreted by cells. Nevertheless, the optimization of cell-based therapy demands the development of alternative strategies to improve cell administration. Alginate has been used for cell microencapsulation, due to its simple gelling process, excellent biocompatibility, biodegradability properties and its stability under in vivo conditions. On the other hand, nanoparticulate systems have been widely used in the biomedical field, as drug delivery devices that can improve the efficiency and widening the applications of the microencapsulation systems. Therefore, the present study aimed to develop biodegradable alginate microparticles that were used for human fibroblasts cells and chitosan nanoparticles encapsulation, in order to improve the wound healing process. To do so, two types of microparticles were firstly produced with alginate and a mixture of alginate and collagen. Subsequently, these carriers were characterized according to their size and geometry by scanning electron microscopy. Confocal images were also acquired to confirm cell encapsulation in microparticles. The cytotoxic profile of the carriers was assessed. Cell release from microparticles was observed over time after encapsulation through optical microscopic analysis. In second part of the work, chitosan nanoparticles loaded with a model protein (bovine serum albumin) were produced and were incorporated in microparticles. The encapsulation efficiency of this protein in nanoparticles was determined. Then, both the morphology and size of these nanoparticles were characterized. The results herein obtained showed that the developed microparticles and nanoparticles can be used as systems tailored for sustainable cells and drug release.As lesões na pele são acontecimentos traumáticos que levam ao aumento da perda de fluidos, a infecções, à formação de cicatrizes e ao aparecimento de regiões imunocomprometidas. Estas feridas podem ser causadas por desordens de origem genética, traumas ou mesmo devido a cirurgias. Deste modo, uma área substancial da pele pode ser danificada, muitas vezes sem a possibilidade de regeneração. Os investigadores têm procurado desenvolver novos sistemas de entrega de drogas, de forma a acelerar o processo de cicatrização. O microencapsulamento celular surgiu recentemente como uma nova abordagem, para entrega controlada e de longa duração de agentes terapêuticos produzidos e secretados pelas próprias células, tais como componentes da matriz extracelular e factores de crescimento, os quais são essenciais para a regeneração. Esta tecnologia tem por base a imobilização de células, dentro de uma matriz polimérica rodeada por uma membrana semi-permeável. Assim, as células não são reconhecidas pelo sistema imunitário do hospedeiro e a membrana permite a difusão de nutrientes e gases para o interior da matriz e a saída das moléculas bioactivas secretadas pelas células e dos resíduos resultantes do metabolismo celular. No entanto, a terapia celular necessita ainda de ser optimizada. O alginato é um polímero que tem sido usado para o encapsulamento celular, devido ao seu fácil processo de gelificação, excelente biocompatibilidade, biodegradabilidade e estabilidade in vivo. Por outro lado, os sistemas nanoparticulados têm sido amplamente utilizados em aplicações biomédicas, por exemplo na produção de dispositivos de entrega direcionada de moléculas bioactivas, uma vez que permitem obter um perfil de libertação controlado. O presente trabalho teve como objectivo o desenvolvimento de micropartículas de alginato para encapsular fibroblastos humanos e nanopartículas de quitosano, com o intuito de futuramente serem usadas como agentes promotores da cicatrização de feridas. Inicialmente, foram produzidos dois tipos de micropartículas, um à base de alginato e outro de alginato com colagénio. As micropartículas produzidas foram caracterizadas quanto ao seu tamanho e geometria por microscopia electrónica de varrimento. Posteriormente, foram também adquiridas imagens de confocal para confirmar o encapsulamento de células nas micropartículas. O perfil citotóxico dos transportadores foi caracterizado através de testes de viabilidade celular, os quais confirmaram a biocompatibilidade dos transportadores. O perfil de libertação das células foi observado por análise microscópica ao longo dos dias. Numa segunda parte do trabalho foram produzidas nanopartículas de quitosano com o objetivo de serem incorporadas nas micropartículas como transportadores de factores de crescimento e, assim, favorecer a cicatrização das feridas. A eficiência de encapsulação das nanopartículas foi avaliada através da incorporação de uma proteína modelo, albumina de soro bovino. Posteriormente fez-se a caracterização da morfologia e do tamanho destas nanopartículas. Os estudos efectuados demonstraram que o sistema desenvolvido é adequado para a libertação de células e moléculas bioativas de forma controlada, prolongada e em concentrações fisiológicas.
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Vickers, Lance Alan. "Predicting Regeneration in Appalachian Hardwood Stands Using the REGEN Expert System". Thesis, Virginia Tech, 2009. http://hdl.handle.net/10919/34785.
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A study was initiated to adapt the REGEN regeneration prediction model to the Appalachians of Virginia and West Virginia. REGEN generates predictions via expert created REGEN knowledge bases (RKBs) that contain competitive rankings and stochastic parameters for selected species and size classes of advance reproduction. We developed RKBs for four site productivity classes (xeric, subxeric, submesic, mesic), and tested two (subxeric and submesic) using field collected inventory data in this study. To test the model we collected data from 48 paired sites which contained a mature stand and an adjacent regenerating stand (clearcut) of similar site productivity harvested within the past 20 years. Across all 48 sites, model predictions were within 5% of measured values on average, and explained 32% (R2 = 0.32) of the variation in species composition in regenerating stands.
The species compositions of 41 of the paired stands on the Appalachian Plateau in West Virginia were further analyzed to compare species composition. Species composition was compared between the mature and regenerating stands in the subxeric and submesic site classes. A comparison of the upper canopy (dominant and codominant) species composition in regenerating stands to that of all stems â ¥ 1.5 in dbh in the mature stands was conducted as well. Our results suggest that the future species composition of stands regenerating following clearcut harvests will likely differ from previous rotations with mesophytic, shade intolerant species being more numerous. Oaks will likely assume a smaller role as the clearcuts mature, particularly on the submesic sites.Master of Science
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Gaihre, Bipin. "Cellulose-chitosan based Scaffolds as Robust Injectable System for Bone Regeneration". University of Toledo / OhioLINK, 2019. http://rave.ohiolink.edu/etdc/view?acc_num=toledo155653074590645.
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Gangatharan, Girisaran. "Role of Tall and the immune system during zebrafish heart regeneration". Thesis, Montpellier, 2016. http://www.theses.fr/2016MONTT076.
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Contrairement aux mammifères, le poisson zèbre a la capacité de régénérer son coeur après une blessure importante. Une meilleure compréhension de ce phénomène pourrait contribuer à la conception de thérapies cliniques pour améliorer la régénération cardiaque des mammifères. Dans cette étude, nous avons identifié des voies de signalisation de la régénération cardiaque du poisson zèbre utilisant un criblage génétique chimique. De plus, nous avons identifier la présence d'un facteur de transcription bHLH, TAL1 et nous avons montrer son importance au cours de la régénération cardiaque du poisson zèbre. Enfin, nous avons examiner le rôle du système immunitaire lors de la régénération cardiaque du poisson zèbre et nous avons montrer que la sécrétion des metalloprotéinases (MMP14) par les macrophages permettait la dissolution du caillot sanguin, processus nécessaire pour la réussite de la régénération cardiaqueUnlike mammals, zebrafish have the ability to regenerate their heart after substantial injury. A deeper understanding of this phenomenon could aid in the design of clinical therapies to enhance mammalian cardiac regeneration. In this study, we have identified signaling pathways in zebrafish heart regeneration using a chemical genetic screen. Furthermore, we identify the presence of a bHLH transcription factor, Tal1 and show its requirement during zebrafish cardiac regeneration. Finally, we examine the role of the immune system during zebrafish heart regeneration and demonstrate a model of scar removal by MMP14 positive macrophages and show that this process is required for successful heart regeneration to occur
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Baiget, Orts María Amparo. "HYALURONAN BASED BIOMATERIALS FOR CENTRAL NERVOUS TISSUE REGENERATION". Doctoral thesis, Universitat Politècnica de València, 2012. http://hdl.handle.net/10251/14576.
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The aim of this Thesis is to investigate the use of hyaluronic acid as a material for the design of scaffolds aimed at CNS regeneration. The motivation comes from the need of searching for new strategies that allow regeneration in the central nervous system. In degenerative diseases, such as Parkinson's disease, where the progressive loss of neuronal subpopulations occurs, a permissive environment able to support regeneration and connectivity of neurons from the host tissue may be a promising therapy to recover lost functionalities. In this Thesis we have focused on the development of structures able to integrate within the brain, supporting neural cells attachment and survival.
We hypothesized that hyaluronic acid provides an enabling environment and appropriate for regeneration due to its biocompatibility and diverses physiological applications. Biocompatible hydrogels based on modified hyaluronic acid were synthesized. Covalently crosslinked hyaluronic acid hydrogels, alone or in combination with acrylic polymers, were synthesized and permitted to develop different porous structures which may serve in different applications as cell supply, cell repopulation or tissue regeneration. Highly porous with interconnected spherical pores, hollow tubes or multichanneled scaffolds were developed. The processes allow for a wide range of shapes for different applications within the scope of central nervous system regeneration.
Furthermore, in vitro culture of human cell lines together with biomaterials was performed. A human microvascular endothelial cell line (hCMEC/D3) and a human glioma cell line (U373) were chosen for the studies. Experiments were focused on the interaction between hyaluronan based scaffolds and those cell lines composing the blood-brain-barrier (BBB) in the central nervous system. Biocompatibility, viability and phenotype characteristics were assessed.Baiget Orts, MA. (2012). HYALURONAN BASED BIOMATERIALS FOR CENTRAL NERVOUS TISSUE REGENERATION [Tesis doctoral no publicada]. Universitat Politècnica de València. https://doi.org/10.4995/Thesis/10251/14576
Palancia
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Makridakis, Jennifer Lynn. "Braided Collagen Microthreads as a Cell Delivery System in Bioengineered Muscle Regeneration". Digital WPI, 2010. https://digitalcommons.wpi.edu/etd-theses/1112.
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"Engineered muscle tissue offers a promising solution for the treatment of large muscle defects. Three-dimensional tissue engineered matrices, such as microthreads, can be used to grow new myofibers that will reduce scar formation and integrate easily into native myofibers. We hypothesize that adsorbing growth factors to the surface of braided collagen scaffolds using crosslinking strategies will promote muscle derived fibroblastic cell (MDFC) attachment and growth, which will serve as a platform for delivering cells to large muscle defects for muscle regeneration. To test this hypothesis, self-assembled type I collagen threads were braided and crosslinked using 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC) with and without heparin and 5 ng/mL, 10 ng/mL, or 50 ng/mL fibroblast growth factor (FGF-2) bound to the surface. Using immunhistochemistry, braided collagen scaffolds showed the presence of FGF-2 on the surface, and braiding the microthreads increased the mechanical properties compared to single threads. To determine the effect of FGF-2 on MDFC attachment, growth, and alignment, scaffolds were seeded with a MDFC cell suspension for 4 hours using a PDMS mold with a sealed 1 mm by 12 mm channel and cultured for 1, 5, or 7 days. After 1 day of culture, the results show a significant increase in cell attachment on braids crosslinked with EDC/NHS with heparin and no significant difference in attachment between the different concentrations of FGF-2 and EDC/NHS crosslinked scaffolds. After 7 days in culture, the MDFCs responded to FGF-2 with a positive linear correlation between growth rate and concentration of FGF-2 on the surface. Additionally, all control scaffolds showed cellular alignment after 7 days, while MDFCs on FGF-2 modified scaffolds showed limited alignment. These results show braided collagen scaffolds crosslinked with EDC/NHS with heparin delivering a controlled quantity of FGF-2 can support MDFC attachment and growth, which may serve as an exciting new approach to facilitate the growth and ultimately the delivery of cells to large defects in muscle regeneration."
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Avakian, A. "3D-bioprinting system for fabrication of alginate microfibers". Thesis, АНПРЭ, ХНУРЭ, Издательство «Точка», 2017. http://openarchive.nure.ua/handle/document/4074.
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The given work is devoted to the theme of 3D-printing of alginate microfibers with encapsulated stem cells which are used for the nerve regeneration. The motorized printing device is developed and tested. The appropriate software controls the device and allows to design the printing structures.
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徐思慧 i Sze-wai Chui. "The influences of intrinsic and extrinsic factors on the axonal regeneration of embryonic and adult dorsal root ganglion neurons: a cryoculture study". Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 1998. http://hub.hku.hk/bib/B31215208.
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Lau, Chi-yan Jane, i 劉至欣. "Brain derived neurotrophic factors (BDNF) and seprafilm® adhesion barrier on sciatic nerve regeneration in rats". Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2009. http://hub.hku.hk/bib/B42924789.
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Fung, Chun-kit, i 馮俊傑. "In vitro and in vivo studies of skin-derived Schwann cells in nerve regeneration". Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2010. http://hub.hku.hk/bib/B43936027.
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Fung, Chun-kit. "In vitro and in vivo studies of skin-derived Schwann cells in nerve regeneration". Click to view the E-thesis via HKUTO, 2010. http://sunzi.lib.hku.hk/hkuto/record/B43936027.
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游思維 i Siwei You. "Neuronal survival and axonal regeneration of retinal ganglion cells inadult hamsters". Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 1998. http://hub.hku.hk/bib/B3123799X.
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Lowenger, Elizabeth. "Studies of early neural regeneration in the visual system of the goldfish". Thesis, McGill University, 1986. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=66031.
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El-Samadony, Yasser Abdel Fattah. "Simulation of liquid desiccant regeneration for an energy efficient air conditioning system". Thesis, University of Leeds, 2007. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.441236.
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Vargas, Mauricio Enrique. "Control of axon regeneration and wallerian degeneration by the humoral immune system /". May be available electronically:, 2008. http://proquest.umi.com/login?COPT=REJTPTU1MTUmSU5UPTAmVkVSPTI=&clientId=12498.
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Wilkin, Linda Diane. "Rehabilitative influence of therapeutic ultrasound treatment on cellular markers of skeletal muscle regeneration following blunt contusion injury /". The Ohio State University, 2002. http://rave.ohiolink.edu/etdc/view?acc_num=osu1486463321624146.
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You, Siwei. "Neuronal survival and axonal regeneration of retinal ganglion cells in adult hamsters /". Hong Kong : University of Hong Kong, 1998. http://sunzi.lib.hku.hk/hkuto/record.jsp?B19859946.
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Jha, Balendu Shekhar. "Utilization of structural and biochemical cues to enhance peripheral nerve regeneration". VCU Scholars Compass, 2011. http://scholarscompass.vcu.edu/etd/2650.
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This study examines the prospects of using the electrospinning process to fabricate tissue engineering scaffolds targeting a variety of regenerative applications, with a primary focus on the production of nerve guides for the treatment of long-defect nerve injuries in the peripheral nervous system. A basic overview of the conventional electrospinning process is provided, and the utility of this fabrication scheme in the production of collagen-based tissue engineering scaffolds is demonstrated. Next, a novel modification of the basic electrospinning process is presented. This process, called two pole air gap electrospinning, was developed to produce nerve guides that exhibit an anisotropic structure that mimics the extracellular matrix of native peripheral nerve tissue. This electrospinning process makes it possible to produce macroscopic nerve guides that are cylindrical in shape and composed of dense arrays of nano- to micron-scale diameter fibers. Unlike, conventional hollow core nerve guides, these electrospun constructs lack a central lumen, hence the designation 3D (for three-dimensional) nerve guide. The fibers are nearly exclusively arrayed in parallel with the long axis of the construct. This architectural feature provides thousands of individual channels, and aligned fibers that provide guidance cues that are designed to drive regenerating axons to grow in a highly directed fashion down the longitudinal axis of the guide. To supplement the structural cues provided by the fibrillar arrays of the electrospun 3D nerve guides, an alginate-based platform designed to deliver therapeutic reagents was developed and characterized. This platform makes it possible to fabricate gradients of therapeutic reagents within the fibrillar arrays of an electrospun nerve guide. Functional and structural analyses of these constructs supplemented with or without a gradient of NGF, in a long-defect nerve injury in the rodent sciatic nerve indicate that the 3D design is superior to the gold standard treatment, the autologous nerve graft. Animals treated with the 3D grafts recovered motor and sensory function faster and exhibited far higher nerve-to-nerve and nerve-to-muscle signal amplitudes in electrophysiological studies than animals treated with autologous grafts or conventional hollow core cylindrical grafts.