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Artykuły w czasopismach na temat "Real-time PCR"

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RAZA, ABIDA, i NAUREEN A KHATTAK. "REAL TIME PCR;". Professional Medical Journal 19, nr 06 (3.11.2012): 751–59. http://dx.doi.org/10.29309/tpmj/2012.19.06.2455.

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In recent years, real-time PCR has come forward as a robust and widely used molecular technique in clinical and biologicalsettings. Although it can detect very minute quantities of target nucleic acid, but quantification of specific nucleic acids is not an easy task.Accurate and precise quantification is hampered by a number of factors that may include assay development and validation, fluorophoresselection, handling during sample preparation, storage, reaction procedures, and batch analysis conditions. Even minor variations aresignificantly magnified by the exponential nature of this technique. Current review gives an insight of the advantages, limitations, assaychemistries, quantitation parameters, and quality control issues related to this technology. Moreover it will also highlight the utilization of Realtime PCR in clinical oncology, virology, microbiology, and gene expression studies.
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Lederman, Lynne. "Real-Time PCR". BioTechniques 44, nr 2 (luty 2008): 179–83. http://dx.doi.org/10.2144/000112741.

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Davidson, Eugene A. "REAL-TIME PCR". Shock 27, nr 6 (czerwiec 2007): 708. http://dx.doi.org/10.1097/01.shk.0000270193.65250.8e.

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Kusser, Wolfgang, Sandrine Javorschi i Martin A. Gleeson. "Real-Time PCR". Cold Spring Harbor Protocols 2006, nr 1 (1.01.2006): pdb.prot4112. http://dx.doi.org/10.1101/pdb.prot4112.

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Fraga, Dean, Tea Meulia i Steven Fenster. "Real-Time PCR". Current Protocols Essential Laboratory Techniques 00, nr 1 (styczeń 2008): 10.3.1–10.3.34. http://dx.doi.org/10.1002/9780470089941.et1003s00.

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Busch, Ulrich. "Real-Time PCR". Journal für Verbraucherschutz und Lebensmittelsicherheit 2, nr 2 (maj 2007): 111–12. http://dx.doi.org/10.1007/s00003-007-0178-7.

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Kang, Won, Sang-Bum Park, Youn-Hyoung Nam, Young-Chang An, Sang-Hyun Lee, Won-Cheoul Jang, Su-Min Park, Jong-Wan Kim i Song-Chun Chong. "Detection of Hepatitis B Virus Using Micro-PCR and Real-Time PCR Methods". Journal of the Korean Chemical Society 51, nr 1 (20.02.2007): 36–42. http://dx.doi.org/10.5012/jkcs.2007.51.1.036.

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Heid, C. A., J. Stevens, K. J. Livak i P. M. Williams. "Real time quantitative PCR." Genome Research 6, nr 10 (1.10.1996): 986–94. http://dx.doi.org/10.1101/gr.6.10.986.

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Schmittgen, Thomas D. "Real-Time Quantitative PCR". Methods 25, nr 4 (grudzień 2001): 383–85. http://dx.doi.org/10.1006/meth.2001.1260.

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Gospodinović, Hristina, Ljiljana Pavlović, Marija Obradović, Sanja Dimitrijević, Sofija Jovanović i Edita Grego. "Detection of high-risk HPV genotypes using Real-time PCR". Glasnik javnog zdravlja 96, nr 4 (2022): 416–26. http://dx.doi.org/10.5937/serbjph2204416g.

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Discovery of the causal relationship between the human papilloma virus and cervical cancer formation increased the significance of the real-time PCR in HPV diagnostics. Based on evidence showing that they caused cervical cancer, 14 HPV types have been classified as carcinogenic (HPV16, 18, 31, 33, 35, 39, 45, 51, 52, 56, 58, 59, 66 and 68). This study analysed cervical smears taken from female patients, aged 19 to 25 years, using the Viasure diagnostic test for the detection of high-risk HPV genotypes and individual identification of HPV genotypes 16 and 18. A total of 110 cervical smears were analysed and 44 positive samples were detected (40%). DNA analysis of the positive samples found the following distribution of the HPV types: 27% HPV (31, 39, 56); 22% HPV (52, 59, 68); 18% HPV16; 13% HPV (33, 45, 51); 12% HPV (35, 58, 66); 8% HPV18. This study and the high positivity rate it found indicate that there is a lack of awareness among the youth on the measures of prevention, as well as a lack of understanding of HPV infection.
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Rozprawy doktorskie na temat "Real-time PCR"

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Crane, Bryan Lee 1976. "Real time PCR measurement by fluorescence anisotropy". Thesis, Massachusetts Institute of Technology, 2004. http://hdl.handle.net/1721.1/30347.

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Thesis (Ph. D.)--Massachusetts Institute of Technology, Dept. of Mechanical Engineering, February 2005.
Page 230 blank.
Includes bibliographical references (p. 181-190).
Real-time polymerase chain reaction (PCR) is the gold-standard for quantitation in both mutation and gene expression analyses. Already this technique has found valuable clinical application in disease diagnosis and progression evaluation. As the number of known gene-disease correlations continues to rise, there will be increased demand for higher throughput and decreased cost for these analyses. Present real-time PCR measurement is based upon the fluorescent intensity of either intercalating dyes or oligonucleotide probes. Intercalating dye methods suffer from a lack of binding specificity, while probe methods are expensive and require increased assay optimization. In this thesis, a new method is presented for monitoring real-time PCR that utilizes the fluorescent anisotropy (FA) of labeled primers. FA, when measured at constant temperature, is indicative of the molecular mass to which the fluorophore is attached. Specificity is improved with the FA method over the use of intercalating dyes since the selective binding of primers is required for signal change. Assay complexity and cost are reduced compared to fluorogenic probe methods since the probes are eliminated. The design of a prototype instrument, which successfully implements this new method, is presented. Instrument and assay performance are compared to intercalating dye assays run in commercially available instrumentation. Theoretical limits on performance are also presented and compared to experimental results. Excellent repeatability and linearity are observed with respect to these benchmarks. This new method, having both high specificity and low optimization complexity, is expected to be particularly applicable to the demanding robustness requirements of nano-scale PCR.
by Bryan Lee Crane.
Ph.D.
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Rozales, Franciéli Pedrotti. "Real time-pcr e nested-pcr no diagnóstico da tuberculose pulmonar". reponame:Biblioteca Digital de Teses e Dissertações da UFRGS, 2013. http://hdl.handle.net/10183/72990.

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A tuberculose (TB) é um importante problema de saúde pública, portanto, é necessário o desenvolvimento de novas ferramentas para a detecção rápida e confiável do Mycobacterium tuberculosis, prevenindo a transmissão da TB. O objetivo deste estudo foi avaliar dois testes moleculares para detecção de bactérias do Complexo Mycobacterium tuberculosis (MTBC) diretamente de amostras clínicas. Foi realizado um estudo transversal, no qual foram selecionadas 124 amostras respiratórias. As amostras foram avaliadas por dois testes moleculares “in house” para detecção do MTBC: NESTED-PCR (NPCR) e Real Time PCR (RT-PCR) ambos com primers para o IS6110. As amostras também foram avaliadas por um teste direto (pesquisa de BAAR). Os resultados foram comparados com a cultura para M.tuberculosis e também com os resultados da cultura juntamente com dados clínicos. Uma amostra comercial com quantificação de DNA conhecida foi utilizada para determinação do Limite de Detecção (LOD) dos testes moleculares. O LOD foi de 1 cópia/μL para o RT-PCR e de 25 cópias/μL para o NPCR. O BAAR apresentou baixa sensibilidade - SE - (40%) e alta especificidade - SP - (94%). Ambos os ensaios moleculares, apresentaram elevada SE e SP (RT-PCR 98% e 91%, NPCR 86% e 93%, respectivamente) em relação à cultura. Quando comparamos os resultados frente a cultura juntamente com os dados clínicos, a SE e a SP foram de 90% e 97% para a RT-PCR e de 80% e 99% para a NPCR, respectivamente. Houve um pequeno decréscimo da SE dos métodos moleculares, quando comparados com a cultura mais os dados clínicos em relação à cultura isoladamente, no entanto, a SP foi consideravelmente elevada para os três métodos avaliados. Avaliamos o custo dos insumos para os ensaios moleculares: o custo do NPCR foi de $ 17.77/teste enquanto que o custo do RT-PCR foi de $ 15.76/teste. Em relação ao tempo de execução dos testes o RT-PCR foi mais rápido (2 horas) do que o NPCR (4 horas). Este estudo confirma que técnicas de PCRs podem ser muito úteis para o diagnóstico rápido da TB respiratória, com altas taxas de SP. Ele também pode ser muito importante para a exclusão de diagnóstico, considerando o alto VPN encontrados no nosso estudo. Os resultados demonstraram que os ensaios moleculares visando IS6110 do M. tuberculosis podem ajudar a melhorar o diagnóstico da TB pulmonar, com muitos potenciais efeitos positivos para a gestão clínica e de controle da doença.
Tuberculosis (TB) remains as an important public health problem worldwide. Therefore, the rapid detection of M. tuberculosis is of primary importance to effectively reduce transmission among patients. The aims of this study were to evaluate two molecular tests to detect M. tuberculosis complex (MTBC) directly from clinical samples. The study included 124 respiratory samples which were evaluated by two in house molecular assays for MTBC detection: Nested PCR (NPCR) and Real Time PCR (RT-PCR). The respiratory samples were also evaluated by the direct test (AFB assay). The results were compared with the results of culture and also compared with the culture results plus clinical data of patients. We used a commercial DNA sample with known quantification to establish the Limit of Detection (LOD). The LOD was 1 copy/μL for RT-PCR and 25 copies/μL for NPCR. The AFB assay presented low sensitivity – SE - (40%) and a high specificity - SP – (94%). Both molecular assays, RT-PCR and NPCR presented high SE and SP (RT-PCR 98% and 91%, NPCR 86% and 93%, respectively) compared to culture. When the results of the molecular tests were compared to the culture plus clinical data the SE and SP were 90,20% and 97,26% for RT-PCR and 80,39% and 98,63% for the NPCR, respectively. It was possible to observe a slight decrease of SE of the molecular methods in comparison to culture plus clinical data in relation to culture; however, the SP was increased, since many cases of TB could not be confirmed by culture. Furthermore we evaluated the cost of molecular assays: the NPCR cost was $17.77/test while the RT-PCR cost was $15.76/test. The RT-PCR test was faster (2 hours) than the NPCR (4 hours) to be performed. Our study confirms that PCRs may be useful for rapid diagnosis of respiratory TB, with high SP rates. It may also be very important to exclude such diagnosis, considering the high NPV found in our study. In summary, PCRs targeting IS6110 of MTB improve the accuracy of the diagnosis of pulmonary TB, with many potential positive effects for clinical management and control of the disease.
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Pires, Elisabete Sofia Videira. "Real-Time PCR, High Resolution Melting - aplicações forenses". Master's thesis, Universidade de Aveiro, 2012. http://hdl.handle.net/10773/10747.

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Mestrado em Biologia Molecular e Celular
Apontada como a maior revolução científica na área forense desde a descoberta das impressões digitais, a identificação humana por meio da análise do DNA tornou-se uma poderosa ferramenta de investigação, auxiliando na elucidação de casos forenses, baseando-se cientificamente na existência de polimorfismos genéticos ao longo do genoma em indivíduos diferentes, que faz com que cada pessoa possua um código genético único. Com a introdução da real-time PCR nas investigações forenses, tornou-se possível uma análise sensível e específica de regiões polimórficas tanto no genoma nuclear como no mitocondrial, a partir de quantidades ínfimas de DNA obtidas de amostras altamente degradadas ou com baixo número de cópias. A quantificação do DNA é um procedimento importante na análise forense e deve ser efetuado, previamente, a qualquer análise de DNA. A união entre a bioinformática e a genética forense propiciou a criação de métodos de análise específicos, como a HRM, muito útil na genotipagem de SNPs, de extrema importância na investigação forense. Foi elaborada uma revisão bibliográfica com o objetivo de conhecer as aplicações forenses da real-time PCR e os respetivos métodos, tendo se confirmado então a aplicabilidade deste método na área forense.
Listed as the greatest revolution in forensic science since the discovery of fingerprints, identification by analyzing human DNA has become a powerful research tool, helping to elucidate forensic cases, scientifically based on the existence of genetic polymorphisms throughout the genome at different individuals, which causes that each person has a unique genetic code. With the introduction of real-time PCR in forensic investigations, it became possible a sensitive and specific analysis of polymorphic regions both in the mitochondrial and nuclear genome, from minute quantities of DNA obtained from samples highly degraded or low copy number. The quantification of DNA is an important procedure in forensic analysis and must be made in advance to any DNA analysis. The union between forensic genetics and bioinformatics led to the creation of specific analysis methods, such as HRM, very useful in scanning and genotyping of SNPs, of utmost importance in forensic investigation. A literature review has been prepared in order to meet the forensic applications of real-time PCR and related methods, and so been confirmed the applicability of this method in the forensic field.
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Dunkley, Kingsley Delroy. "Modulation of cell yields and genetic responses of Salmonella fermentation and colonization in the gastrointestinal ecology of avian species". [College Station, Tex. : Texas A&M University, 2006. http://hdl.handle.net/1969.1/ETD-TAMU-1187.

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Andalo, Alice. "Analisi quantitativa dell'espressione genica mediante real-time rt-pcr". Bachelor's thesis, Alma Mater Studiorum - Università di Bologna, 2015. http://amslaurea.unibo.it/8450/.

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Il primo capitolo di questo lavoro di tesi introduce i concetti di biologia necessari per comprendere il fenomeno dell’espressione genica. Il secondo capitolo descrive i metodi e le tecniche di laboratorio utilizzate per ottenere il cDNA, il materiale genetico che verrà amplificato nella real-time PCR. Nel terzo capitolo si descrive la tecnica di real-time PCR, partendo da una descrizione della PCR convenzionale fino a delineare le caratteristiche della sua evoluzione in real-time PCR. Si prosegue con la spiegazione del principio fisico alla base della tecnica e delle molecole necessarie (fluorofori e sonde) per realizzarla; infine si descrive l’hardware e il software dello strumento. Il quarto capitolo presenta le tecniche di analisi del segnale che utilizzano metodi di quantificazione assoluta o relativa. Infine nel quinto capitolo è presentato un caso di studio, cioè un’analisi di espressione genica con real-time PCR condotta durante l’esperienza di tirocinio presso il laboratorio ICM. e delle molecole necessarie (fluorofori e sonde) per realizzarla; infine si descrive l’hardware e il software dello strumento. Il quarto capitolo presenta le tecniche di analisi del segnale che utilizzano metodi di quantificazione assoluta o relativa. Infine nel quinto capitolo è presentato un caso di studio, cioè un’analisi di espressione genica con real-time PCR condotta durante l’esperienza di tirocinio presso il laboratorio ICM.
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Dörries, Hans-Henno. "Entwicklung von Real-Time-PCR-Nachweissystemen für getränkerelevante Hefen". [S.l.] : [s.n.], 2006. http://deposit.ddb.de/cgi-bin/dokserv?idn=979663938.

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Hartmann, Britta. "Entwicklung einer Real-time-PCR-Nachweismethode für Yersinia enterocolitica". [S.l.] : [s.n.], 2007. http://edoc.ub.uni-muenchen.de/archive/00006660.

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Hartmann, Britta. "Entwicklung einer Real-Time PCR-Nachweismethode für Yersinia enterocolitica". Diss., lmu, 2007. http://nbn-resolving.de/urn:nbn:de:bvb:19-66603.

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Malatji, Dikeledi Petunia. "Detection of Babesia rossi genotypes using real-time PCR". Diss., University of Pretoria, 2011. http://hdl.handle.net/2263/31138.

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Babesia rossi is the most virulent Babesia species in dogs occurring in South Africa and is associated with severe clinical manifestation and mortalities. Babesia rossi is highly pathogenic and reacting dogs requires treatment to prevent mortalities. Mild, uncomplicated forms of the disease are effectively treated with antibabesial drugs. Complicated forms of the disease are difficult to treat with high mortality rate.This results in the disease being of economic importance in South Africa. There is a lack of information regarding the relationship between parasite genotype and disease phenotype. The broad objective of this study is to detect B. rossi genotypes using real-time PCR. This test is a method that can be used to monitor amplicon formation throughout the PCR reaction and to estimate the initial concentration of target DNA in samples. Polymerase chain reaction, sequencing and phylogenetic analysis were used to determine the genotype detection of Babesia rossi isolated from infected dogs in South Africa. The correlation between the parasite genotype and disease phenotype was also investigated. Correlation between B. rossi Erythrocyte Membrane Antigen (BrEMA1) genotypes and age of dogs was studied in 44 cases. A total of 101 blood samples were tested using the reverse line blot (RLB) assay. Ninety six percent hybridized to the Babesia rossi species-specific probe. Our findings demonstrate that the most encountered (BrEMA1) genotype is genotype29, followed by genotype28 and genotype19, with genotype29 associated with most of the severe clinical signs diagnosed compared to genotype19. The number of cases caused by genotype19 was low, constituting 22% of the cases. This is comparable with the 2009 report where genotype19 appeared highly prevalent and virulent, whereas the prevalence of genotype28/29 appeared moderate. In this dissertation, we present the first report on the detection of an amplification product of BrEMA1 genotypes using real-time PCR. Samples which were below the detectable limit of conventional PCR and could not be sequenced probably due to low parasitaemia were also used and real-time PCR provided the ability to detect B. rossi positive animals. This was able to detect 10 BrEMA1 genotypes. However, it was not reliable enough in differentiating between various BrEMA1 genotypes. When evaluating the relationship between BrEMA1 genotypes, clinical manifestation and age of the dogs, collapse was found to be a poor prognostic sign in dogs with babesiosis. Genotype29 was associated with most of the collapsed cases and with high number of the dogs that died. Although B. rossi can infect dogs of all ages, young dogs showed to be more susceptible to canine babesiosis than older dogs. This is in agreement with the survey carried out from the Onderstepoort Veterinary Academic Hospital (OVAH) in 1994. Since B. rossi is the most pathogenic species of the large babesias of dogs, the ability to manage the disease is dependent on rapid detection of the organism. Real-time PCR test is indeed a quicker method to confirm diagnoses of B. rossi infected dogs. It can detect B. rossi infection at a low DNA concentration (0.185 ng/μl) which provides a major advantage in detecting B. rossi infection in field blood samples.
Dissertation (MSc)--University of Pretoria, 2011.
Veterinary Tropical Diseases
MSc
Unrestricted
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Zhang, Yan. "Frequent RASSF1A gene promoter hypermethylation in breast cancer". [S.l. : s.n.], 2008. http://nbn-resolving.de/urn:nbn:de:bsz:289-vts-63611.

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Książki na temat "Real-time PCR"

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Tevfik, Dorak M., red. Real-time PCR. New York: Taylor & Francis, 2006.

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Tevfik, Dorak M., red. Real-time PCR. New York: Taylor & Francis, 2006.

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Biassoni, Roberto, i Alessandro Raso, red. Quantitative Real-Time PCR. New York, NY: Springer New York, 2014. http://dx.doi.org/10.1007/978-1-4939-0733-5.

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Biassoni, Roberto, i Alessandro Raso, red. Quantitative Real-Time PCR. New York, NY: Springer New York, 2020. http://dx.doi.org/10.1007/978-1-4939-9833-3.

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Meuer, Stefan, Carl Wittwer i Kan-Ichi Nakagawara, red. Rapid Cycle Real-Time PCR. Berlin, Heidelberg: Springer Berlin Heidelberg, 2001. http://dx.doi.org/10.1007/978-3-642-59524-0.

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Quantitative real-time PCR in applied microbiology. Norfolk, UK: Caister Academic Press, 2012.

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Julie, Logan, Edwards Kirstin i Saunders Nick, red. Real-time PCR: Current technology and applications. Norfolk, UK: Caister Academic Press, 2009.

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Quantitative real-time PCR: Methods and protocols. New York: Humana Press, 2014.

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Dietmaier, Wolfgang, Carl Wittwer i Natarajan Sivasubramanian, red. Rapid Cycle Real-Time PCR — Methods and Applications. Berlin, Heidelberg: Springer Berlin Heidelberg, 2002. http://dx.doi.org/10.1007/978-3-642-59397-0.

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Wittwer, Carl, Meinhard Hahn i Karen Kaul, red. Rapid Cycle Real-Time PCR — Methods and Applications. Berlin, Heidelberg: Springer Berlin Heidelberg, 2004. http://dx.doi.org/10.1007/978-3-642-18840-4.

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Części książek na temat "Real-time PCR"

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Müller, Hans-Joachim, i Daniel Ruben Prange. "Real-Time-PCR". W PCR - Polymerase-Kettenreaktion, 65–76. Berlin, Heidelberg: Springer Berlin Heidelberg, 2015. http://dx.doi.org/10.1007/978-3-662-48236-0_14.

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Mathew, Alan G. "Real-Time PCR". W Handbook of Food Safety Engineering, 217–57. Oxford, UK: Wiley-Blackwell, 2012. http://dx.doi.org/10.1002/9781444355321.ch10.

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Saunders, Nicholas A. "Real-Time PCR". W Genomics, Proteomics, and Clinical Bacteriology, 191–211. Totowa, NJ: Humana Press, 2004. http://dx.doi.org/10.1385/1-59259-763-7:191.

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Evrard, A., N. Boulle i G. s. Lutfalla. "Real-Time PCR". W Nanoscience, 841–69. Berlin, Heidelberg: Springer Berlin Heidelberg, 2009. http://dx.doi.org/10.1007/978-3-540-88633-4_15.

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Konrad, Regina, i Ulrich Busch. "PCR und Real-Time PCR". W Molekularbiologische Methoden in der Lebensmittelanalytik, 35–47. Berlin, Heidelberg: Springer Berlin Heidelberg, 2010. http://dx.doi.org/10.1007/978-3-642-10716-0_4.

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Sluijter, J. P. G., G. Pasterkamp i D. P. V. de Kleijn. "Quantitative Real-Time PCR". W Cardiovascular Research, 75–83. Boston, MA: Springer US, 2006. http://dx.doi.org/10.1007/0-387-23329-6_4.

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Broll, Hermann. "Quantitative Real-Time PCR". W Molecular Biological and Immunological Techniques and Applications for Food Chemists, 59–83. Hoboken, NJ, USA: John Wiley & Sons, Inc., 2010. http://dx.doi.org/10.1002/9780470637685.ch3.

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Bartholomew, Rachel A., Janine R. Hutchison, Timothy M. Straub i Douglas R. Call. "PCR, Real-Time PCR, Digital PCR, and Isothermal Amplification". W Manual of Environmental Microbiology, 2.3.2–1–2.3.2–13. Washington, DC, USA: ASM Press, 2015. http://dx.doi.org/10.1128/9781555818821.ch2.3.2.

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Schmittgen, Thomas D., Eun Joo Lee i Jinmai Jiang. "High-Throughput Real-Time PCR". W Methods in Molecular Biology, 89–98. Totowa, NJ: Humana Press, 2008. http://dx.doi.org/10.1007/978-1-60327-040-3_7.

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Dotti, Isabella, Ermanno Nardon, Danae Pracella i Serena Bonin. "Quantitative Real-Time RT-PCR". W Guidelines for Molecular Analysis in Archive Tissues, 121–32. Berlin, Heidelberg: Springer Berlin Heidelberg, 2011. http://dx.doi.org/10.1007/978-3-642-17890-0_25.

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Streszczenia konferencji na temat "Real-time PCR"

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Becker, Holger, Nadine Hlawatsch, Richard Klemm, Christian Moche, Thomas Hansen-Hagge i Claudia Gärtner. "Real-time PCR in microfluidic devices". W SPIE MOEMS-MEMS, redaktorzy Bonnie L. Gray i Holger Becker. SPIE, 2014. http://dx.doi.org/10.1117/12.2037241.

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Woudenberg, Timothy M., i J. Stevens. "Quantitative PCR by real-time detection". W Photonics West '96, redaktorzy Gerald E. Cohn, Steven A. Soper i C. H. Winston Chen. SPIE, 1996. http://dx.doi.org/10.1117/12.237619.

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BARLOCCHI, G., U. MASTROMATTEO, S. SASSOLINI, M. SCURATI i F. VILLA. "MICROFLUIDIC DEVICE FOR REAL TIME PCR DETECTION". W Proceedings of the 9th Italian Conference. WORLD SCIENTIFIC, 2005. http://dx.doi.org/10.1142/9789812701770_0058.

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GANDELMAN, O. A., V. L. CHURCH, C. A. MOORE, C. CARNE, H. JALAL, J. A. H. MURRAY i L. C. TISI. "BART – BIOLUMINESCENT ALTERNATIVE TO REAL-TIME PCR". W Chemistry, Biology and Applications. WORLD SCIENTIFIC, 2007. http://dx.doi.org/10.1142/9789812770196_0023.

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Chen, Ping-Hei, Da-Sheng Lee, Jui-Hung Chien i Meng-Hsun Wu. "Development of a Novel Real-Time PCR Machine". W European Conference on Biomedical Optics. Washington, D.C.: OSA, 2005. http://dx.doi.org/10.1364/ecbo.2005.wg7.

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Kubista, Mikael, Anders Stalberg i Tzachi Bar. "Light-up-probe-based real-time Q-PCR". W BiOS 2001 The International Symposium on Biomedical Optics, redaktorzy Ramesh Raghavachari i Weihong Tan. SPIE, 2001. http://dx.doi.org/10.1117/12.424589.

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Hertwig, Aline Morgan von, Maristela da Silva do Nascimento, Maria Helena Pelegrinelli Fungaro i Marta Hiromi Taniwaki. "Real-Time Pcr for Identification of Aspergillus Niger". W XII Latin American Congress on Food Microbiology and Hygiene. São Paulo: Editora Edgard Blücher, 2014. http://dx.doi.org/10.5151/foodsci-microal-077.

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Lee, D. S., J. H. Chien, M. H. Wu i P. H. Chen. "Development of a novel real-time PCR machine". W European Conference on Biomedical Optics 2005, redaktor Christian D. Depeursinge. SPIE, 2005. http://dx.doi.org/10.1117/12.633056.

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"RqPCRAnalysis: Analysis of Quantitative Real-time PCR Data". W International Conference on Bioinformatics Models, Methods and Algorithms. SciTePress - Science and and Technology Publications, 2013. http://dx.doi.org/10.5220/0004312002020211.

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Gaertner, Claudia, Holger Becker, Nadine Hlawatsch, Richard Klemm, Christian Moche, René Sewart, Rainer Frank i Andreas Willems. "Lab-on-a-chip PCR: real time PCR in miniaturized format for HLA diagnostics". W SPIE Sensing Technology + Applications, redaktorzy Brian M. Cullum i Eric S. McLamore. SPIE, 2014. http://dx.doi.org/10.1117/12.2050233.

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Raporty organizacyjne na temat "Real-time PCR"

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Dilcheva, Valeria, Ivelin Vladov i Svetlozara Petkova. A Comparative Study of Six Trichinella Species by Real-time PCR Assay. "Prof. Marin Drinov" Publishing House of Bulgarian Academy of Sciences, styczeń 2018. http://dx.doi.org/10.7546/crabs.2018.01.08.

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Dilcheva, Valeria, Ivelin Vladov i Svetlozara Petkova. A Comparative Study of Six Trichinella Species by Real-time PCR Assay. "Prof. Marin Drinov" Publishing House of Bulgarian Academy of Sciences, styczeń 2018. http://dx.doi.org/10.7546/grabs2018.1.08.

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Harris, D. L. Hank, Isabel Turney Harris, James S. Dickson, Stephen Gaul, Brad T. Bosworth i Lori Feldmann. Quantitative Real-time PCR (qPCR) for the Determination of Salmonella Levels in Lairage. Ames (Iowa): Iowa State University, styczeń 2009. http://dx.doi.org/10.31274/ans_air-180814-1009.

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McAvin, James C., i Carl J. Mason. Pre-Clinical Testing of a Real-Time PCR Assay for Diahhreal Disease Agent Cryptosporidium. Fort Belvoir, VA: Defense Technical Information Center, maj 2014. http://dx.doi.org/10.21236/ada600722.

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Bonab, Zahra Hojjati, Parisa Mohammadi, Ezzat Asgarani i Nassim Ghorbanmehr. The Evaluation of Nitrogen Fixation Activity of Soil Cyanobacteria via Reduction Assay and Real-time PCR. "Prof. Marin Drinov" Publishing House of Bulgarian Academy of Sciences, czerwiec 2019. http://dx.doi.org/10.7546/crabs.2019.06.07.

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Faris, Gregory W. Development of Laser-Mediated Nanodroplet Real-Time PCR on Circulating Tumor Cells (CTC) by Microfilter Platform. Fort Belvoir, VA: Defense Technical Information Center, czerwiec 2015. http://dx.doi.org/10.21236/ada621341.

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McAvin, James C., i Carl J. Mason. Norovirus Real Time RT-PCR Detection Technology Transition to the Joint Biological Identification and Diagnosis System (JBAIDS). Fort Belvoir, VA: Defense Technical Information Center, wrzesień 2012. http://dx.doi.org/10.21236/ada568257.

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McAvin, James C., i Carl J. Mason. Pre-Clinical Testing of Real-Time PCR Assays for Diarrheal Disease Agents of Genera Escherichia and Shigella. Fort Belvoir, VA: Defense Technical Information Center, maj 2014. http://dx.doi.org/10.21236/ada600976.

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Hutchison, Janine R., Gregory F. Piepel, Brett G. Amidan, Michael A. Sydor i Brooke L. Deatherage Kaiser. False Negative Rates of a Macrofoam-Swab Sampling Method with Low Surface Concentrations of Two Bacillus anthracis Surrogates via Real-Time PCR. Office of Scientific and Technical Information (OSTI), maj 2015. http://dx.doi.org/10.2172/1186982.

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Hutchison, Janine R., Gregory F. Piepel, Brett G. Amidan, Michael A. Sydor i Brooke L. D. Kaiser. False Negative Rates of a Macrofoam-Swab Sampling Method with Low Surface Concentrations of Two Bacillus anthracis Surrogates via Real-Time PCR. Office of Scientific and Technical Information (OSTI), czerwiec 2016. http://dx.doi.org/10.2172/1260869.

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