Gotowe bibliografie tematyczne / Pyocyanin

Gotowa bibliografia na temat „Pyocyanin”

Autor: Grafiati

Data publikacji: 4 czerwca 2021

Data aktualizacji: 21 lutego 2023

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Artykuły w czasopismach na temat "Pyocyanin"

O'Malley, Yunxia Q., Maher Y. Abdalla, Michael L. McCormick, Krzysztof J. Reszka, Gerene M. Denning i Bradley E. Britigan. "Subcellular localization ofPseudomonaspyocyanin cytotoxicity in human lung epithelial cells". American Journal of Physiology-Lung Cellular and Molecular Physiology 284, nr 2 (1.02.2003): L420—L430. http://dx.doi.org/10.1152/ajplung.00316.2002.

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The Pseudomonas aeruginosa secretory product pyocyanin damages lung epithelium, likely due to redox cycling of pyocyanin and resultant superoxide and H2O2generation. Subcellular site(s) of pyocyanin redox cycling and toxicity have not been well studied. Therefore, pyocyanin's effects on subcellular parameters in the A549 human type II alveolar epithelial cell line were examined. Confocal and electron microscopy studies suggested mitochondrial redox cycling of pyocyanin and extracellular H2O2release, respectively. Pyocyanin decreased mitochondrial and cytoplasmic aconitase activity, ATP levels, cellular reduction of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide, and mitochondrial membrane potential. These effects were transient at low pyocyanin concentrations and were linked to apparent cell-mediated metabolism of pyocyanin. Overexpression of MnSOD, but not CuZnSOD or catalase, protected cellular aconitase, but not ATP, from pyocyanin-mediated depletion. This suggests that loss of aconitase activity is not responsible for ATP depletion. How pyocyanin leads to ATP depletion, the mechanism of cellular metabolism of pyocyanin, and the impact of mitochondrial pyocyanin redox cycling on other cellular events are important areas for future study.

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O'Malley, Yunxia Q., Krzysztof J. Reszka, George T. Rasmussen, Maher Y. Abdalla, Gerene M. Denning i Bradley E. Britigan. "ThePseudomonassecretory product pyocyanin inhibits catalase activity in human lung epithelial cells". American Journal of Physiology-Lung Cellular and Molecular Physiology 285, nr 5 (listopad 2003): L1077—L1086. http://dx.doi.org/10.1152/ajplung.00198.2003.

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Pyocyanin, produced by Pseudomonas aeruginosa, has many deleterious effects on human cells that relate to its ability to generate reactive oxygen species (ROS), such as superoxide and hydrogen peroxide. Human cells possess several mechanisms to protect themselves from ROS, including manganese superoxide dismutase (MnSOD), copper zinc superoxide dismutase (CuZnSOD), and catalase. Given the link between pyocyanin-mediated epithelial cell injury and oxidative stress, we assessed pyocyanin's effect on MnSOD, CuZnSOD, and catalase levels in the A549 human alveolar epithelial cell line and in normal human bronchial epithelial cells. In both cell types, CuZnSOD and MnSOD were unaltered, but over 24 h pyocyanin significantly decreased cellular catalase activity and protein content. Pyocyanin also decreased catalase mRNA. Overexpression of MnSOD in A549 cells prevented pyocyanin-mediated loss of catalase protein, but catalase activity still declined. Furthermore, pyocyanin decreased catalase activity, but not protein, in A549 cells overexpressing human catalase. These data suggest a direct effect of pyocyanin on catalase activity. Addition of pyocyanin to catalase in a cell-free system also decreased catalase activity. Mammalian catalase binds four NADPH molecules, helping maintain enzyme activity. Spin-trapping data suggest that pyocyanin directly oxidizes this NADPH, producing superoxide. We conclude that pyocyanin may decrease cellular catalase activity via both transcriptional regulation and direct inactivation of the enzyme. Decreased cellular catalase activity and failure to augment MnSOD could contribute to pyocyanin-dependent cytotoxicity.

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O'Malley, Yunxia Q., Krzysztof J. Reszka, Douglas R. Spitz, Gerene M. Denning i Bradley E. Britigan. "Pseudomonas aeruginosapyocyanin directly oxidizes glutathione and decreases its levels in airway epithelial cells". American Journal of Physiology-Lung Cellular and Molecular Physiology 287, nr 1 (lipiec 2004): L94—L103. http://dx.doi.org/10.1152/ajplung.00025.2004.

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Production of pyocyanin enhances Pseudomonas aeruginosa virulence. Many of pyocyanin's in vitro and in vivo cytotoxic effects on human cells appear to result from its ability to redox cycle. Pyocyanin directly accepts electrons from NADH or NADPH with subsequent electron transfer to oxygen, generating reactive oxygen species. Reduced glutathione (GSH) is an important cellular antioxidant, and it contributes to the regulation of redox-sensitive signaling systems. Using the human bronchial epithelial (HBE) and the A549 human type II alveolar epithelial cell lines, we tested the hypothesis that pyocyanin can deplete airway epithelial cells of GSH. Incubation of both cell types with pyocyanin led to a concentration-dependent loss of cellular GSH (up to 50%) and an increase in oxidized GSH (GSSG) in the HBE, but not A549 cells, at 24 h. An increase in total GSH, mostly as GSSG, was detected in the culture media, suggesting export of GSH or GSSG from the pyocyanin-exposed cells. Loss of GSH could be due to pyocyanin-induced H2O2formation. However, overexpression of catalase only partially prevented the pyocyanin-mediated decline in cellular GSH. Cell-free electron paramagnetic resonance studies revealed that pyocyanin directly oxidizes GSH, forming pyocyanin free radical and O2−·. Pyocyanin oxidized other thiol-containing compounds, cysteine and N-acetyl-cysteine, but not methionine. Thus GSH may enhance pyocyanin-induced cytotoxicity by functioning as an alternative source of reducing equivalents for pyocyanin redox cycling. Pyocyanin-mediated alterations in cellular GSH may alter epithelial cell functions by modulating redox sensitive signaling events.

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فاطمة حسين بريج مورد, رغد أكرم عزيز i محمد فرج شذر. "العلاقة التأزرية لصبغة البايوسيانين pyocyaninالمنتجة من بكتريا Pseudomonasaeruginosa و خميرة Saccharomyces cerevisiaeتجاه بعض انواع البكتريا المرضية و المعزولة من عينات سريرية متعددة". journal of the college of basic education 22, SI (24.08.2022): 237–57. http://dx.doi.org/10.35950/cbej.v22isi.5898.

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تضمنت الدراسة تأكيد تشخيص 29 عزلة من بكتريا Pseudomonasaeruginosaبالأعتماد على الفحوص الزرعية و المجهرية و الكيموحيوية , وعزلتين منخميرة Saccharomyces cerevisiae, فضلا عن جمع 12 عزلة مرضية من عينات سريرية متعددة من بعض مستشفيات مدينة الطب شملت كلا من Staphylococcusaureus , Escherichia coli, و تم تأكيد تشخيص جميع العزلات بنظام VITEK2 وحسب الكارت الخاص بالبكتريا السالبة و الموجبة لصبغة كرام بالإضافة للكارت الخاص بالخميرة ,ثم اجريت غربلة لبكتريا Pseudomonasaeruginosaالمعزولة من الجروح و الحروقو القشع لغرض اختيار العزلة الأكفأ في انتاج صبغة البايوسيانين pyocyanin اذ بينت النتائج ان عزلة الجروح التي تمتلك اعلى تركيز و سجلت اعلى درجة امتصاصية هي الافضل في انتاج صبغة البايوسيانين pyocyanin , اختبرت حساسية البكتريا المرضية تجاه 10 انواع من المضادات الحياتية , وبينت النتائج ان اغلب العزلات مقاومة لمضادات (Ciprofloxacin, Imipenem, Amikacin) و متباينة التأثير لبقية المضادات , هذا و استعملت خمائر الخبز الجافة للحصول على عزلات خميرة Saccharomyces cerevisiae التي شملت كل من العزلةBy 1 من الخميرة Super mayaالصينية المنشأ والعزلة By2 من خميرة Glorpan الصينية المنشأ .كما أجريت غربلة لعزلات خميرة S.cerevisiae من اجل انتخاب العزلة الاكفأ في انتاج البروتينات المثبطة بإستعمال طريقة الانتشار في الحفر لمعرفة تأثير هذه المواد تجاه البكتريا المرضية قيد الدراسة , و قد اظهرت النتائج ان العزلة By 1 أعطت اعلى معدل تثبيط تجاه العزلات S .aureus , E .coli من العزلة By 2. ايضا تمقياس التركيز المثبط الادنى MIC لكل من صبغة البايوسيانين Pyocyaninو راشح خميرة S.cerevisiae تجاه العزلات البكتيرية قيد الدراسة و بإستعمال طريقة العكارة في الانابيب , واثبتت النتائج ان قيمة MIC كانت متباينة حسب تركيز الراشح و فعاليته و حسب تدرج البكتريا المرضية . كما درست العلاقة التأزرية بين صبغة البايوسيانين Pyocyanin و راشح S.cerevisiae تجاه البكتريا المرضية التي تم اختيارها لهذا الاختبار حسب درجة مقاومتها للمضادات الحيوية ,اذ اظهرت النتائج ان العلاقة كانت تآزرية و لا وجود لأي علاقة اخرى بسبب فعالية الصبغة مع الراشح على تثبيط فعل البكتريا المرضية . لذلك توصي الدراسة على التركيز على صبغة البايوسيانين Pyocyaninمع خميرةSaccharomycescerevisiae (كخلية كاملة او المواد المثبطة التي تنتجها ( علاجاللإصابات التي تسببها بعض انواع البكتريا المرضية .

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Jain, Preeti, Suman Kumari i Ashish Malik. "INHIBITION OF PYOCYANIN PRODUCTION IN PSEUDOMONAS AERUGINOSA BY NATURAL ANTIMICROBIAL COMPOUNDS FROM HERBAL EXTRACTS". Asian Journal of Pharmaceutical and Clinical Research 10, nr 6 (1.06.2017): 389. http://dx.doi.org/10.22159/ajpcr.2017.v10i6.18056.

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Objective: P. aeruginosa produces a range of metabolites including pyocyanin that enhance its ability to resist antibiotics and becomes capable of surviving adverse conditions.Method: In this report, eight plants (extracted with five solvents) were screened for antibacterial activity against P. aeruginosa by microbroth dilution method. Afterward tested for inhibition of pyocyanin in presence and absence of plant extracts.Result: Among these D. muricata and S. quitoenes exhibited good antibacterial potential. Inhibition of pyocyanin is identified as a potential anti-virulence strategy. Therefore, acetone extract of six plants exhibited MIC ≤3.125mg/ml and methanol extract of three plants exhibited MIC ≤6.25mg/ml were used to check pyocyanin inhibition in P. aeruginosa. In acetone extracts, significant pyocyanin inhibition was found in I. pestigirdis and C. colocynthis. In methanol extracts, C. colocynthis and D. muricata showed considerable pyocyanin inhibition.Conclusion: Overall result indicates that the best antimicrobial compound (growth inhibitor) may not be best inhibitor of pyocyanin biosynthesis or vice-versa. Moreover, I. pestigirdis, C. colocynthis and D. muricata seems to contain compounds which inhibit the growth of bacteria as well as the biosynthesis of pyocyanin.

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De Vleesschauwer, David, Pierre Cornelis i Monica Höfte. "Redox-Active Pyocyanin Secreted by Pseudomonas aeruginosa 7NSK2 Triggers Systemic Resistance to Magnaporthe grisea but Enhances Rhizoctonia solani Susceptibility in Rice". Molecular Plant-Microbe Interactions® 19, nr 12 (grudzień 2006): 1406–19. http://dx.doi.org/10.1094/mpmi-19-1406.

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Pseudomonas aeruginosa 7NSK2 induces resistance in dicots through a synergistic interaction of the phenazine pyocyanin and the salicylic acid-derivative pyochelin. Root inoculation of the monocot model rice with 7NSK2 partially protected leaves against blast disease (Magnaporthe grisea) but failed to consistently reduce sheath blight (Rhizoctonia solani). Only mutations interfering with pyocyanin production led to a significant decrease in induced systemic resistance (ISR) to M. grisea, and in trans complementation for pyocyanin production restored the ability to elicit ISR. Intriguingly, pyocyanin-deficient mutants, unlike the wild type, triggered ISR against R. solani. Hence, bacterial pyocyanin plays a differential role in 7NSK2-mediated ISR in rice. Application of purified pyocyanin to hydroponically grown rice seedlings increased H2O2 levels locally on the root surface as well as a biphasic H2O2 generation pattern in distal leaves. Co-application of pyocyanin and the antioxidant sodium ascorbate alleviated the opposite effects of pyocyanin on rice blast and sheath blight development, suggesting that the differential effectiveness of pyocyanin with respect to 7NSK2-triggered ISR is mediated by transiently elevated H2O2 levels in planta. The cumulative results suggest that reactive oxygen species act as a double-edged sword in the interaction of rice with the hemibiotroph M. grisea and the necrotroph R. solani.

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Pragana, Luiz Gustavo, Carlos Eduardo Tavares Norat, Diogo Simas Bernardes Dias, Elisângela Afonso de Moura Kretzschmar, Rafael de Almeida Travassos i Ulrich Vasconcelos. "Summary of the role of pyocyanin in the transformation and biodegradation of Polycyclic Aromatic Hydrocarbons". Conjecturas 22, nr 15 (3.11.2022): 405–29. http://dx.doi.org/10.53660/conj-1874-2p12.

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Pyocyanin is an active redox phenazine of intense blue color and specific to Pseudomonas aeruginosa. The synthesis of the molecule confers different benefits to the bacterium. Pyocyanin can control its growth and persistence in environments with high nutritional pressures, forming biofilms. As well, synthesis of pyocyanin enables P. aeruginosa tolerate and uptake highly toxic compounds such as polycyclic aromatic hydrocarbons (PAHs), considered the most dangerous compounds among all molecules found in crude oil and petroderivatives. Additionally, pyocyanin increases the bioavailability of PAHs and its metabolites are used to synthetize crucial molecules for the biodegradation of other PAHs. On the other hand, oil hydrocarbons can serve as oxygen vectors during the synthesis of pyocyanin, contributing to the sustainability of the biodegradation process. This review is a compilation of recent advances reported in the literature about the relationship between pyocyanin expression and the hydrocarbonoclastic activity of P. aeruginosa. This characteristic, for the pyocyanin-deficient strains, is important for the degradation of PAHs, a topic that has been unevenly studied.

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Price-Whelan, Alexa, Lars E. P. Dietrich i Dianne K. Newman. "Pyocyanin Alters Redox Homeostasis and Carbon Flux through Central Metabolic Pathways in Pseudomonas aeruginosa PA14". Journal of Bacteriology 189, nr 17 (25.05.2007): 6372–81. http://dx.doi.org/10.1128/jb.00505-07.

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ABSTRACT The opportunistic pathogen Pseudomonas aeruginosa produces colorful, redox-active antibiotics called phenazines. Excretion of pyocyanin, the best-studied natural phenazine, is responsible for the bluish tint of sputum and pus associated with P. aeruginosa infections in humans. Although the toxicity of pyocyanin for other bacteria, as well as its role in eukaryotic infection, has been studied extensively, the physiological relevance of pyocyanin metabolism for the producing organism is not well understood. Pyocyanin reduction by P. aeruginosa PA14 is readily observed in standing liquid cultures that have consumed all of the oxygen in the medium. We investigated the physiological consequences of pyocyanin reduction by assaying intracellular concentrations of NADH and NAD+ in the wild-type strain and a mutant defective in phenazine production. We found that the mutant accumulated more NADH in stationary phase than the wild type. This increased accumulation correlated with a decrease in oxygen availability and was relieved by the addition of nitrate. Pyocyanin addition to a phenazine-null mutant also decreased intracellular NADH levels, suggesting that pyocyanin reduction facilitates redox balancing in the absence of other electron acceptors. Analysis of extracellular organic acids revealed that pyocyanin stimulated stationary-phase pyruvate excretion in P. aeruginosa PA14, indicating that pyocyanin may also influence the intracellular redox state by decreasing carbon flux through central metabolic pathways.

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Norman, R. Sean, Peter Moeller, Thomas J. McDonald i Pamela J. Morris. "Effect of Pyocyanin on a Crude-Oil-Degrading Microbial Community". Applied and Environmental Microbiology 70, nr 7 (lipiec 2004): 4004–11. http://dx.doi.org/10.1128/aem.70.7.4004-4011.2004.

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ABSTRACT Pseudomonas aeruginosa is an n-alkane degrader that is frequently isolated from petroleum-contaminated sites and produces factors that enhance its competitiveness and survival in many environments. In this study, one such factor, pyocyanin, has been detected in an oil-degrading culture containing P. aeruginosa and is a redox-active compound capable of inhibiting microbial growth. To examine the effects of pyocyanin further, an oil-degrading culture was grown with and without 9.5 μM pyocyanin and microbial community structure and oil degradation were monitored for 50 days. Denaturing gradient gel electrophoresis (DGGE) analysis of cultures revealed a decrease in the microbial community diversity in the pyocyanin-amended cultures compared to that of the unamended cultures. Two members of the microbial community in pure culture exhibited intermediate and high sensitivities to pyocyanin corresponding to intermediate and low levels of activity for the antioxidant enzymes catalase and superoxide dismutase, respectively. Another member of the community that remained constant in the DGGE gels over the 50-day culture incubation period exhibited no sensitivity to pyocyanin, corresponding to a high level of catalase and superoxide dismutase when examined in pure culture. Pyocyanin also affected the overall degradation of the crude oil. At 50 days, the culture without pyocyanin had decreased polycyclic aromatic hydrocarbons compared to the pyocyanin-amended culture, with a specific reduction in the degradation of dibenzothiophenes, naphthalenes, and C29 and C30 hopanes. This study demonstrated that pyocyanin influenced the diversity of the microbial community and suggests the importance of understanding how interspecies interactions influence the degradation capability of a microbial community.

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Cheluvappa, Rajkumar, Ronald Shimmon, Michael Dawson, Sarah N. Hilmer i David G. Le Couteur. "Reactions of Pseudomonas aeruginosa pyocyanin with reduced glutathione." Acta Biochimica Polonica 55, nr 3 (17.09.2008): 571–80. http://dx.doi.org/10.18388/abp.2008_3063.

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Pseudomonas aeruginosa is the most common cause of chronic and recurrent lung infections in patients with cystic fibrosis (CF) whose sputa contain copious quantities of P. aeruginosa toxin, pyocyanin. Pyocyanin triggers tissue damage mainly by its redox cycling and induction of reactive oxygen species (ROS). The reactions between reduced glutathione (GSH) and pyocyanin were observed using absorption spectra from spectrophotometry and the reaction products analysed by nuclear magnetic resonance imaging. Pyocyanin reacted with GSH non-enzymatically at 37 degrees C resulting in the production of red-brown products, spectophotometrically visible as a 480 nm maximum absorption peak after 24 h of incubation. The reaction was concentration-dependent on reduced glutathione but not on pyocyanin. Minimizing the accessibility of oxygen to the reaction decreased its rate. The anti-oxidant enzyme catalase circumvented the reaction. Proton-NMR analysis demonstrated the persistence of the original aromatic ring and the methyl-group of pyocyanin in the red-brown products. Anti-oxidant agents having thiol groups produced similar spectophotometrically visible peaks. The presence of a previously unidentified non-enzymatic GSH-dependent metabolic pathway for pyocyanin has thus been identified. The reaction between pyocyanin and GSH is concentration-, time-, and O(2)-dependent. The formation of H(2)O(2) as an intermediate and the thiol group in GSH seem to be important in this reaction.

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Rozprawy doktorskie na temat "Pyocyanin"

Price-Whelan, Alexa Mari Leadbetter Jared R. Newman Dianne K. "Physiology and mechanisms of pyocyanin reduction in pseudomonas aeruginosa /". Diss., Pasadena, Calif. : Caltech, 2009. http://resolver.caltech.edu/CaltechETD:etd-02182009-100346.

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Larian, Nika. "PYOCYANIN, A VIRULENCE FACTOR PRODUCED BY SEPSIS-CAUSING PSEUDOMONAS AERUGINOSA, PROMOTES ADIPOSE WASTING AND CACHEXIA". UKnowledge, 2019. https://uknowledge.uky.edu/pharmacol_etds/31.

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Sepsis is a leading cause of death among critically ill patients that results in metabolic alterations including hypercatabolism, lipoatrophy, and muscle wasting, contributing to the development of cachexia. Septic cachexia is associated with loss of body weight, fat mass, and lean mass and dysregulated immune function. There are currently no efficacious treatment strategies for septic cachexia, and nutritional interventions have limited success in preventing hypercatabolic wasting. Pyocyanin is a virulence factor produced by sepsis-causing Pseudomonas aeruginosa that has been shown to activate the aryl hydrocarbon receptor (AhR), increase inflammation, and produce reactive oxygen species. Thus, pyocyanin represents a novel mechanistic target in the development of septic cachexia. In Aim 1, we hypothesized that pyocyanin reduces adipocyte differentiation and activates AhR in vitro and in vivo. In vitro, pyocyanin reduced differentiation of 3T3-L1 cells to adipocytes and promoted expression of proinflammatory cytokines. These effects were associated with activation of AhR. We established an in vivo model of pyocyanin-induced cachexia using repeat intraperitoneal exposure to pyocyanin in male and female C57BL/6J mice. Acutely, pyocyanin reduced differentiation of stem cells isolated from adipose stromal vascular tissue and augmented expression of proinflammatory cytokines. Chronically, pyocyanin reduced body weight and fat mass, which was associated with adipose-specific AhR activation. Pyocyanin had sexually dimorphic effects on lipolysis and adipocyte inflammation. These data suggest a role of pyocyanin in adipose cachexia associated with sepsis. In Aim 2, we hypothesized that pyocyanin activates adipocyte AhR to promote adipose tissue wasting and cachexia. To test this hypothesis, we used a mouse model of adipocyte-specific deficiency of AhR and chronically administered pyocyanin to male and female mice. In male mice with adipocyte AhR deficiency, effects of pyocyanin to promote adipose wasting and cachexia were attenuated. In contrast, female adipocyte AhR deficient mice had an augmented response to pyocyanin to decrease body weight. Results suggest divergent mechanisms of pyocyanin to regulate adiposity and body weight through adipocyte AhR between male and female mice. These data support a role for pyocyanin in the development of adipose cachexia associated with Pseudomonas aeruginosa sepsis that is partially regulated by adipocyte AhR. Targeting pyocyanin’s effects on adipocytes represents a potentially novel therapeutic approach for septic cachexia that could mitigate septic cachexia, a condition associated with increased risk of mortality in this population.

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Pan, Ninyuan, i 潘寧遠. "The effects of pseudomonas aeruginosa pyocyanin on interleukin-8 expression in bronchial epithelium and therapeutic implications inbronchiectasis". Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2006. http://hub.hku.hk/bib/B45012866.

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Wally, Hassan. "Identification and Characterization of MvaT and MvaU Global Regulators in Arginine Catabolism and Quorum Sensing of Pseudomonas aeruginosa". Digital Archive @ GSU, 2007. http://digitalarchive.gsu.edu/biology_diss/16.

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Arginine utilization in P. aeruginosa as the source of carbon, nitrogen, and energy is controlled by a complicated regulatory mechanism. While ArgR in the presence of exogenous arginine is required for auto-induction of the aotJQMOP-argR operon for arginine uptake and regulation, this operon is subjected to carbon catabolite repression via an unknown mechanism. This study demonstrated that succinate exerted a stronger repression effect than glucose or pyruvate on arginine induction of an aotJ::lacZ fusion in wild type PAO1. Expression of the aotJ::lacZ fusion was analyzed against three different backgrounds, cbrAB, crc, and vfr, that have been suggested to play a role in carbon catabolite repression. These mutations exerted a negative effect on the arginine-responsive induction to different extents, with the order of cbrAB > vfr > crc. A series of aotJ::lacZ fusions with deletions in the aotJ regulatory region were constructed and the effect of exogenous arginine examined in the argR mutant and its parent strain. The results indicated that a 250-bp sequence upstream of the ArgR operator is required for the optimal induction of the operon by exogenous arginine, and revealed the presence of a cryptic promoter (P0) in this region. Electromobility shift assays with crude cell-free extracts of PAO1 revealed that a DNA-binding protein other than ArgR binds to the aforementioned 250-bp region. Through reverse genetics, two regulatory proteins MvaT and MvaU were identified and specifically interacted with the aotJ-argR regulatory region. The MvaT/MvaU are involved in the regulation of the P0 promoter. The importance of MvaT and MvaU for bacterial growth was supported by the notion that no true mvaT mvaU double knockout mutant can be constructed. This is the first case to characterize the growth phenotypes of quasi-mvaT mvaU double mutants complemented with fusions for arginine-inducible expression of mvaT or mvaU. Further analysis of the physiological significance of MvaT and MvaU revealed their involvement in swarming response and pyocyanin synthesis. The defect in pyocyanin synthesis was correlated to the diminished level of PQS, an important chemical signal compound in the quorum sensing network of P. aeruginosa.

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Pan, Ninyuan. "The effects of pseudomonas aeruginosa pyocyanin on interleukin-8 expression in bronchial epithelium and therapeutic implications in bronchiectasis /". View the Table of Contents & Abstract, 2006. http://sunzi.lib.hku.hk/hkuto/record/B36432660.

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SILVA, Tacilene Luzia da. "Atividade sinérgica do timol e agentes antimicrobianos frente à Pseudomonas aeruginosa multirresistente e seus efeitos sobre a biossíntese de biofilme e piocianina". Universidade Federal de Pernambuco, 2015. https://repositorio.ufpe.br/handle/123456789/16574.

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Submitted by Haroudo Xavier Filho (haroudo.xavierfo@ufpe.br) on 2016-04-14T18:46:13Z No. of bitstreams: 2 license_rdf: 1232 bytes, checksum: 66e71c371cc565284e70f40736c94386 (MD5) Dissertação Tacilene Luzia Silva.pdf: 1235794 bytes, checksum: f6e18d8a1d865ebf8536ae8b8a1666f3 (MD5)
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Pseudomonas aeruginosa é uma bactéria Gram negativa, oportunista e ubíqua, frequentemente associada a infecções graves em pacientes imunocomprometidos. Em razão do aumento de resistência dessa bactéria aos múltiplos antimicrobianos, surgem à preocupação e a procura por novas alternativas terapêuticas, com as substâncias bioativas de origem natural representando uma importante fonte para obtenção desses medicamentos. O objetivo do presente estudo foi determinar a atividade sinérgica do timol e agentes antimicrobianos frente a cepas de Pseudomonas aeruginosa multirresistentes e avaliar os efeitos dessa interação sobre a biossíntese de biofilme e de piocianina. Para isso, numa primeira etapa foi determinada a concentração inibitória e bactericida mínima do timol e de antimicrobianos (Polimixina B, ceftazidima, piperacilina/tazobactam, cefepima, ciprofloxacino e meropenem) frente a dez cepas de Pseudomonas aeruginosa. O estudo da interação entre o timol e os agentes antimicrobianos foi realizado pelo método do tabuleiro de xadrez. Os critérios utilizados para avaliar a atividade sinérgica foram definidos pelo Índice da Concentração Inibitória Fracionada (FIC índex). A partir dos melhores valores do FIC índex das associações timol/antimicrobiano foram avaliadas a atividade sobre a produção de biofilme e piocianina. Três cepas (LFBM 01, LFBM 02, LFBM 16) apresentaram um perfil de resistência ao meropenem e cefepima e um efeito sinérgico foi observado entre o timol e meropenem ou cefepima sobre essas cepas. A associação timol/cefepima inibiu a biossíntese do biofilme em até 99,76%, e a associação timol/meropenem mostrou ser mais eficaz na inibição da piocianina cujos valores foram de até 84,33%. O timol associado ao meropenem ou cefepima, age sinergicamente, inibindo cepas de Pseudomonas aeruginosa multirresistentes e interferindo na biossíntese de biofilme e piocianina.
Pseudomonas aeruginosa is a Gram negative bacteria, opportunistic and ubiquitous, often associated with severe infections in immunocompromised patients. Due to the increased resistance of the bacteria to multiple antibiotics, there are the concerns and the search for new therapeutic alternatives, with the bioactive substances of natural origin represents an important source for obtaining these drugs. The aim of this study was to determine the synergistic activity of thymol and antimicrobials agents multiresistant Pseudomonas aeruginosa strains and evaluate the effects of this interaction on the biofilm biosynthesis and pyocyanin. For this, a first step was determined and the minimum inhibitory concentration of thymol and bactericidal antibiotics (polymyxin B, ceftazidime, piperacillin / tazobactam, cefepime, ciprofloxacin and meropenem) compared to ten strains of Pseudomonas aeruginosa. The study of the interaction between the thymol and antimicrobial agents was carried out by the checkerboard method. The criteria used to evaluate the synergistic activity were defined by the Index of Fractional Inhibitory Concentration (FIC index). From the best FIC index values of associations thymol / antimicrobial activity were evaluated on the production of biofilm and pyocyanin. Three strains (LFBM 01, LFBM 02, LFBM 16) showed an meropenem resistance profile and cefepime and a synergistic effect was observed between the thymol and meropenem or cefepime on these strains. The thymol / cefepime combination inhibited biofilm biosynthesis up to 99.76%, and thymol association / meropenem was more effective in inhibiting pyocyanin with values of up to 84.33%. The thymol associated with meropenem or cefepime, acts synergistically by inhibiting multidrug-resistant Pseudomonas aeruginosa strains and interfering in the biosynthesis of biofilm and pyocyanin.

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Barakat, Rana. "Etude des propriétés biologiques et antimicrobiennes de la pyocyanine, pigment redox-actif produit par Pseudomonas aeruginosa". Phd thesis, Université de La Rochelle, 2012. http://tel.archives-ouvertes.fr/tel-00825873.

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La pyocyanine (PYO) est une phénazine de couleur bleu-vert, produite spécifiquement par la bactérie pathogène opportuniste Pseudomonas aeruginosa (Pa). La toxicité aérobie de la PYO envers les cellules de mammifères, les levures et les bactéries a été décrite de longue date, mais la compréhension des mécanismes d'action est encore lacunaire, en particulier en conditions de limitation en O2 (conditions rencontrées dans le contexte infectieux). De plus, il a récemment été montré que la PYO peut apporter des effets bénéfiques pour la souche productrice en hypoxie. Au cours de ce travail, nous avons réexaminé les effets de la PYO sur un large panel de bactéries dont son propre producteur (Pa) ainsi que sur un modèle cellulaire eucaryote Saccharomyces cerevisiae exposées à différentes tensions en O2. Nos données suggèrent que la toxicité aérobie de la PYO envers S. cerevisiae est multifactorielle, impliquant à la fois une interaction avec le complexe III de la chaîne respiratoire et l'induction d'un stress oxydatif. Pour la première fois, nous avons mis en évidence une toxicité de la PYO exacerbée en anaérobiose chez un eucaryote (S. cerevisiae). Le mécanisme d'action impliquerait le PYO radical. Nous avons également montré que la PYO peut inhiber la croissance aérobie et anaérobie des microorganismes concurrents, plus particulièrement S. aureus en bloquant le complexe III de la chaîne respiratoire. A l'inverse, la PYO peut stimuler la respiration de Pa surtout dans les conditions mimant le contexte infectieux (hypoxie, vie ralentie). Le complexe III et/ou les oxydases terminales cbb3 serait impliqué favorablement. En conclusion, la PYO jouerait à la fois un rôle de poison hypoxique mais aussi un rôle de navette redox bénéfique pour la survie et la virulence de Pa en hypoxie.

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Cheluvappa, Rajkumar. "Pathophysiology of Liver Sinusoidal Endothelial Cells". Thesis, The University of Sydney, 2008. http://hdl.handle.net/2123/2802.

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Owing to its strategic position in the liver sinusoid, pathologic and morphologic alterations of the Liver Sinusoidal Endothelial Cell (LSEC) have far-reaching repercussions for the whole liver and systemic metabolism. LSECs are perforated with fenestrations, which are pores that facilitate the transfer of lipoproteins and macromolecules between blood and hepatocytes. Loss of LSEC porosity is termed defenestration, which can result from loss of fenestrations and/ or decreases in fenestration diameter. Gram negative bacterial endotoxin (Lipopolysaccharide, LPS) has marked effects on LSEC morphology, including induction LSEC defenestration. Sepsis is associated with hyperlipidemia, and proposed mechanisms include inhibition of tissue lipoprotein lipase and increased triglyceride production by the liver. The LSEC has an increasingly recognized role in hyperlipidemia. Conditions associated with reduced numbers of fenestrations such as ageing and bacterial infections are associated with impaired lipoprotein and chylomicron remnant uptake by the liver and consequent hyperlipidemia. Given the role of the LSEC in liver allograft rejection and hyperlipidemia, changes in the LSEC induced by LPS may have significant clinical implications. In this thesis, the following major hypotheses are explored: 1. The Pseudomonas aeruginosa toxin pyocyanin induces defenestration of the LSEC both in vitro and in vivo 2. The effects of pyocyanin on the LSEC are mediated by oxidative stress 3. Defenestration induced by old age and poloxamer 407 causes intrahepatocytic hypoxia and upregulation of hypoxia-related responses 4. Defenestration of the LSEC seen in old age can be exacerbated by diabetes mellitus and prevented or ameliorated by caloric restriction commencing early in life

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Cheluvappa, Rajkumar. "Pathophysiology of Liver Sinusoidal Endothelial Cells". University of Sydney, 2008. http://hdl.handle.net/2123/2802.

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Doctor of Philosophy(PhD)
Owing to its strategic position in the liver sinusoid, pathologic and morphologic alterations of the Liver Sinusoidal Endothelial Cell (LSEC) have far-reaching repercussions for the whole liver and systemic metabolism. LSECs are perforated with fenestrations, which are pores that facilitate the transfer of lipoproteins and macromolecules between blood and hepatocytes. Loss of LSEC porosity is termed defenestration, which can result from loss of fenestrations and/ or decreases in fenestration diameter. Gram negative bacterial endotoxin (Lipopolysaccharide, LPS) has marked effects on LSEC morphology, including induction LSEC defenestration. Sepsis is associated with hyperlipidemia, and proposed mechanisms include inhibition of tissue lipoprotein lipase and increased triglyceride production by the liver. The LSEC has an increasingly recognized role in hyperlipidemia. Conditions associated with reduced numbers of fenestrations such as ageing and bacterial infections are associated with impaired lipoprotein and chylomicron remnant uptake by the liver and consequent hyperlipidemia. Given the role of the LSEC in liver allograft rejection and hyperlipidemia, changes in the LSEC induced by LPS may have significant clinical implications. In this thesis, the following major hypotheses are explored: 1. The Pseudomonas aeruginosa toxin pyocyanin induces defenestration of the LSEC both in vitro and in vivo 2. The effects of pyocyanin on the LSEC are mediated by oxidative stress 3. Defenestration induced by old age and poloxamer 407 causes intrahepatocytic hypoxia and upregulation of hypoxia-related responses 4. Defenestration of the LSEC seen in old age can be exacerbated by diabetes mellitus and prevented or ameliorated by caloric restriction commencing early in life

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McPhillips, Kathleen Ann. "The role of oxidants in the clearance of apoptotic cells /". Connect to full text via ProQuest. IP filtered, 2006.

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Thesis (Ph.D. in Cancer Biology) -- University of Colorado at Denver and Health Sciences Center, 2006.
Typescript. Includes bibliographical references (leaves 112-124). Free to UCDHSC affiliates. Online version available via ProQuest Digital Dissertations;

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Części książek na temat "Pyocyanin"

Sengupta, Sucharita, i Jayati Bhowal. "Study on the Antioxidant and Cytotoxic Properties of Pyocyanin Extracted from Pseudomonas aeruginosa". W Lecture Notes in Bioengineering, 133–41. Singapore: Springer Singapore, 2020. http://dx.doi.org/10.1007/978-981-15-7409-2_13.

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Jalalimanesh, Ammar, Christina Kuttler, Rodolfo García-Contreras i Judith Pérez-Velázquez. "An Agent-Based Model to Study Selection of Pseudomonas aeruginosa Quorum Sensing by Pyocyanin: A Multidisciplinary Perspective on Bacterial Communication". W Quantitative Models for Microscopic to Macroscopic Biological Macromolecules and Tissues, 133–47. Cham: Springer International Publishing, 2018. http://dx.doi.org/10.1007/978-3-319-73975-5_7.

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Müller, P. K., i P. F. Mühlradt. "Effects of Pyocyanine, Liberated by Pseudomonas aeruginosa, on the Oxidative Burst of Phagocytes". W Immune Consequences of Trauma, Shock, and Sepsis, 195–99. Berlin, Heidelberg: Springer Berlin Heidelberg, 1989. http://dx.doi.org/10.1007/978-3-642-73468-7_24.

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Das, Theerthankar. "Pseudomonas aeruginosa Secreted Biomolecules and Their Diverse Functions in Biofilm Formation and Virulence". W Pseudomonas aeruginosa - Biofilm Formation, Infections and Treatments. IntechOpen, 2021. http://dx.doi.org/10.5772/intechopen.96866.

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Pseudomonas aeruginosa is an opportunistic Gram-negative bacterium accountable for causing life-threatening infections in humans. According to the World Health Organization, P. aeruginosa classified as a critical pathogen. Specifically, P. aeruginosa in its colonized or biofilm state presents a major infection threat to immunocompromised (HIV) patients, Cystic fibrosis, burns, wounds and surgery associated infection. It is also a common pathogen responsible for causing hospital acquired/nosocomial infection and Urinary tract infections. P. aeruginosa biofilm is made up of bacterial self-synthesized biomolecules includes extracellular DNA, polysaccharides, proteins, RNA, siderophores and metabolites such as pyocyanin. This chapter will elaborate the manifold functions of P. aeruginosa secreted biomolecules in establishing and stabilizing biofilms, triggering virulence and pathogenicity in host, and resisting antibiotics and antibacterial agents.

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Das, Theerthankar, Amaye I. Ibugo, William Klare i Mike Manefield. "Role of Pyocyanin and Extracellular DNA in Facilitating Pseudomonas aeruginosa Biofilm Formation". W Microbial Biofilms - Importance and Applications. InTech, 2016. http://dx.doi.org/10.5772/63497.

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Streszczenia konferencji na temat "Pyocyanin"

Mahgoub, Yasmine, Rida Arif i Susu Zughaier. "Pyocyanin pigment from Pseudomonas aeruginosa modulates innate immune defenses in macrophages". W Qatar University Annual Research Forum & Exhibition. Qatar University Press, 2021. http://dx.doi.org/10.29117/quarfe.2021.0137.

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Background: Pseudomonas aeruginosa is a well-known opportunistic pathogen. The gram-negative bacillus, commonly associated with hospital-acquired infections, utilizes the host’s impaired immune responses to establish infection. Of its many virulence factors, pyocyanin is essential for P. aeruginosa to establish its full infectivity. Macrophages act as sentinels of the innate immune system, as well as play other roles in homeostasis, tissue remodeling, and bridging between the innate and adaptive immune systems. Aim: This study aimed to investigate the effects of pyocyanin on macrophage innate immune defenses by assessing the function of macrophages treated with pyocyanin and TLR ligands. Phagocytosis of opsonized zymosan, LPS-induced nitric oxide release and cytokine release were used as measures of functional responses. Results: This study found that pyocyanin inhibited phagocytosis-induced ROS release in a dose-dependent manner and reduced nitric oxide release from macrophages induced with P. aeruginosa LPS. In addition, pyocyanin modulated cytokines and chemokines release from macrophages exposed to P. aeruginosa LPS in a dose-dependent manner. Pyocyanin significantly enhanced IL-1β release as well as several chemokines. Therefore, pyocyanin facilitates Pseudomonas aeruginosa to persevere in the immunocompromised host through modulating macrophage’s innate immune defenses. Conclusion: Pyocyanin inhibits macrophage functional defense responses to facilitate Pseudomonas aeruginosa infection.

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Sudhakar, T., i S. Karpagam. "Antifungal efficacy of pyocyanin produced from bioindicators of nosocomial hazards". W 2011 International Conference on Green Technology and Environmental Conservation (GTEC 2011). IEEE, 2011. http://dx.doi.org/10.1109/gtec.2011.6167673.

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Miyashita, Lisa, Naseem Mushtaq, Michele Padovan, Norrice Liu i Jonathan Grigg. "Exposure to particulate matter increases pyocyanin production in Pseudomonas aeruginosa". W ERS International Congress 2017 abstracts. European Respiratory Society, 2017. http://dx.doi.org/10.1183/1393003.congress-2017.pa3321.

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Wadday, Ali Kadhim, Zeyad A. Saleh i Mohammed Fraj Al-Marjani. "Spectroscopic characteristics and energy transfer of bacterial pigment: (pyocyanin/curcumin)". W SECOND INTERNATIONAL CONFERENCE ON MATERIAL SCIENCE, SMART STRUCTURES AND APPLICATIONS: ICMSS-2019. AIP Publishing, 2019. http://dx.doi.org/10.1063/1.5141438.

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Thrift, William, Arunima Bhattacharjee, Allon I. Hochbaum, Regina Ragan, Cuong Nguyen i Katrine Whiteson. "Robust SERS spectral analysis for quantitative detection of pyocyanin in biological fluids". W Biosensing and Nanomedicine X, redaktorzy Hooman Mohseni, Massoud H. Agahi i Manijeh Razeghi. SPIE, 2017. http://dx.doi.org/10.1117/12.2267958.

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Rashid, Muhammad Ibrahim, i Saadia Andleeb. "Pyocyanin yield improvement for enhancement of Pseudomonas aeruginosa inoculated Microbial Fuel Cell efficiency". W 2018 International Conference on Power Generation Systems and Renewable Energy Technologies (PGSRET). IEEE, 2018. http://dx.doi.org/10.1109/pgsret.2018.8685940.

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Lau, Gee W., Yonghua Hao, Zhizhou Kuang i Brent Walling. "Pseudomonas Aeruginosa Exotoxin Pyocyanin Induces Goblet Cell Hyperplasia And Mucus Hypersecretion By Inactivating Transcription Factor FoxA2". W American Thoracic Society 2011 International Conference, May 13-18, 2011 • Denver Colorado. American Thoracic Society, 2011. http://dx.doi.org/10.1164/ajrccm-conference.2011.183.1_meetingabstracts.a4861.

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Saeed, Ahmed Yas, Sabah Ahmed Naif Aljubori, Mahmood Khlaf Saleh, Mostafa Qahtan Mostafa i Nedaa Wasmi Shehab. "The phenotypic and quantitative detection of the pyocyanin stain in multiple antibiotic resistance Pseudomonas aeruginosa isolated from different clinical infections". W 3RD INTERNATIONAL SCIENTIFIC CONFERENCE OF ALKAFEEL UNIVERSITY (ISCKU 2021). AIP Publishing, 2022. http://dx.doi.org/10.1063/5.0067387.

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