Gotowe bibliografie tematyczne / Protein language models

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Gotowa bibliografia na temat „Protein language models”

Autor: Grafiati

Data publikacji: 26 października 2024

Data aktualizacji: 1 marca 2025

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Artykuły w czasopismach na temat "Protein language models"

Tang, Lin. "Protein language models using convolutions". Nature Methods 21, nr 4 (kwiecień 2024): 550. http://dx.doi.org/10.1038/s41592-024-02252-3.

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Ali, Sarwan, Prakash Chourasia i Murray Patterson. "When Protein Structure Embedding Meets Large Language Models". Genes 15, nr 1 (23.12.2023): 25. http://dx.doi.org/10.3390/genes15010025.

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Protein structure analysis is essential in various bioinformatics domains such as drug discovery, disease diagnosis, and evolutionary studies. Within structural biology, the classification of protein structures is pivotal, employing machine learning algorithms to categorize structures based on data from databases like the Protein Data Bank (PDB). To predict protein functions, embeddings based on protein sequences have been employed. Creating numerical embeddings that preserve vital information while considering protein structure and sequence presents several challenges. The existing literature lacks a comprehensive and effective approach that combines structural and sequence-based features to achieve efficient protein classification. While large language models (LLMs) have exhibited promising outcomes for protein function prediction, their focus primarily lies on protein sequences, disregarding the 3D structures of proteins. The quality of embeddings heavily relies on how well the geometry of the embedding space aligns with the underlying data structure, posing a critical research question. Traditionally, Euclidean space has served as a widely utilized framework for embeddings. In this study, we propose a novel method for designing numerical embeddings in Euclidean space for proteins by leveraging 3D structure information, specifically employing the concept of contact maps. These embeddings are synergistically combined with features extracted from LLMs and traditional feature engineering techniques to enhance the performance of embeddings in supervised protein analysis. Experimental results on benchmark datasets, including PDB Bind and STCRDAB, demonstrate the superior performance of the proposed method for protein function prediction.

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Ferruz, Noelia, i Birte Höcker. "Controllable protein design with language models". Nature Machine Intelligence 4, nr 6 (czerwiec 2022): 521–32. http://dx.doi.org/10.1038/s42256-022-00499-z.

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Li, Xiang, Zhuoyu Wei, Yueran Hu i Xiaolei Zhu. "GraphNABP: Identifying nucleic acid-binding proteins with protein graphs and protein language models". International Journal of Biological Macromolecules 280 (listopad 2024): 135599. http://dx.doi.org/10.1016/j.ijbiomac.2024.135599.

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Singh, Arunima. "Protein language models guide directed antibody evolution". Nature Methods 20, nr 6 (czerwiec 2023): 785. http://dx.doi.org/10.1038/s41592-023-01924-w.

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Tran, Chau, Siddharth Khadkikar i Aleksey Porollo. "Survey of Protein Sequence Embedding Models". International Journal of Molecular Sciences 24, nr 4 (14.02.2023): 3775. http://dx.doi.org/10.3390/ijms24043775.

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Derived from the natural language processing (NLP) algorithms, protein language models enable the encoding of protein sequences, which are widely diverse in length and amino acid composition, in fixed-size numerical vectors (embeddings). We surveyed representative embedding models such as Esm, Esm1b, ProtT5, and SeqVec, along with their derivatives (GoPredSim and PLAST), to conduct the following tasks in computational biology: embedding the Saccharomyces cerevisiae proteome, gene ontology (GO) annotation of the uncharacterized proteins of this organism, relating variants of human proteins to disease status, correlating mutants of beta-lactamase TEM-1 from Escherichia coli with experimentally measured antimicrobial resistance, and analyzing diverse fungal mating factors. We discuss the advances and shortcomings, differences, and concordance of the models. Of note, all of the models revealed that the uncharacterized proteins in yeast tend to be less than 200 amino acids long, contain fewer aspartates and glutamates, and are enriched for cysteine. Less than half of these proteins can be annotated with GO terms with high confidence. The distribution of the cosine similarity scores of benign and pathogenic mutations to the reference human proteins shows a statistically significant difference. The differences in embeddings of the reference TEM-1 and mutants have low to no correlation with minimal inhibitory concentrations (MIC).

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Pokharel, Suresh, Pawel Pratyush, Hamid D. Ismail, Junfeng Ma i Dukka B. KC. "Integrating Embeddings from Multiple Protein Language Models to Improve Protein O-GlcNAc Site Prediction". International Journal of Molecular Sciences 24, nr 21 (6.11.2023): 16000. http://dx.doi.org/10.3390/ijms242116000.

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O-linked β-N-acetylglucosamine (O-GlcNAc) is a distinct monosaccharide modification of serine (S) or threonine (T) residues of nucleocytoplasmic and mitochondrial proteins. O-GlcNAc modification (i.e., O-GlcNAcylation) is involved in the regulation of diverse cellular processes, including transcription, epigenetic modifications, and cell signaling. Despite the great progress in experimentally mapping O-GlcNAc sites, there is an unmet need to develop robust prediction tools that can effectively locate the presence of O-GlcNAc sites in protein sequences of interest. In this work, we performed a comprehensive evaluation of a framework for prediction of protein O-GlcNAc sites using embeddings from pre-trained protein language models. In particular, we compared the performance of three protein sequence-based large protein language models (pLMs), Ankh, ESM-2, and ProtT5, for prediction of O-GlcNAc sites and also evaluated various ensemble strategies to integrate embeddings from these protein language models. Upon investigation, the decision-level fusion approach that integrates the decisions of the three embedding models, which we call LM-OGlcNAc-Site, outperformed the models trained on these individual language models as well as other fusion approaches and other existing predictors in almost all of the parameters evaluated. The precise prediction of O-GlcNAc sites will facilitate the probing of O-GlcNAc site-specific functions of proteins in physiology and diseases. Moreover, these findings also indicate the effectiveness of combined uses of multiple protein language models in post-translational modification prediction and open exciting avenues for further research and exploration in other protein downstream tasks. LM-OGlcNAc-Site’s web server and source code are publicly available to the community.

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Weissenow, Konstantin, i Burkhard Rost. "Are protein language models the new universal key?" Current Opinion in Structural Biology 91 (kwiecień 2025): 102997. https://doi.org/10.1016/j.sbi.2025.102997.

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Wang, Wenkai, Zhenling Peng i Jianyi Yang. "Single-sequence protein structure prediction using supervised transformer protein language models". Nature Computational Science 2, nr 12 (19.12.2022): 804–14. http://dx.doi.org/10.1038/s43588-022-00373-3.

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Kenlay, Henry, Frédéric A. Dreyer, Aleksandr Kovaltsuk, Dom Miketa, Douglas Pires i Charlotte M. Deane. "Large scale paired antibody language models". PLOS Computational Biology 20, nr 12 (6.12.2024): e1012646. https://doi.org/10.1371/journal.pcbi.1012646.

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Antibodies are proteins produced by the immune system that can identify and neutralise a wide variety of antigens with high specificity and affinity, and constitute the most successful class of biotherapeutics. With the advent of next-generation sequencing, billions of antibody sequences have been collected in recent years, though their application in the design of better therapeutics has been constrained by the sheer volume and complexity of the data. To address this challenge, we present IgBert and IgT5, the best performing antibody-specific language models developed to date which can consistently handle both paired and unpaired variable region sequences as input. These models are trained comprehensively using the more than two billion unpaired sequences and two million paired sequences of light and heavy chains present in the Observed Antibody Space dataset. We show that our models outperform existing antibody and protein language models on a diverse range of design and regression tasks relevant to antibody engineering. This advancement marks a significant leap forward in leveraging machine learning, large scale data sets and high-performance computing for enhancing antibody design for therapeutic development.

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Rozprawy doktorskie na temat "Protein language models"

Meynard, Barthélémy. "Language Models towards Conditional Generative Modelsof Proteins Sequences". Electronic Thesis or Diss., Sorbonne université, 2024. http://www.theses.fr/2024SORUS195.

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Nous commençons par examiner ce qui rend un modèle génératif efficace pour les séquences de protéines. Dans notre première étude, "Interpretable Pairwise Distillations for Generative Protein Sequence Models" nous comparons les modèles de réseaux de neurones complexes à des modèles de distributions pair à pair plus simples. Cette comparaison révèle que les modèles plus simples peuvent égaler de près la performance des modèles plus complexes dans la prédiction de l'effet des mutations sur les protéines. Cette découverte remet en question l'hypothèse selon laquelle les modèles plus complexes sont toujours meilleurs, préparant le terrain pour de plus amples explorations.Dans une seconde partie, nous nous penchons sur le conditionnement de séquence avec "Generating Interacting Protein Sequences using Domain-to-Domain Translation" Cette étude introduit une approche novatrice pour générer des séquences de protéines qui peuvent interagir avec d'autres protéines spécifiques. En traitant cela comme un problème de traduction, similaire aux méthodes utilisées dans le traitement du langage naturel, nous créons des séquences avec des fonctionnalités intentionnelles. De plus, nous abordons le défi crucial de la prédiction de l'interaction entre le récepteur des cellules T (TCR) et l'épitope dans "TULIP—a Transformer based Unsupervised Language model for Interacting Peptides and T-cell receptors" Cette étude introduit une approche d'apprentissage non supervisée pour prédire avec précision les liaisons TCR-épitope, surmontant les limitations de qualité des données et les biais de formation inhérents aux modèles précédents. Ces avancées soulignent le potentiel du conditionnement de séquence dans la création de designs de protéines fonctionnellement spécifiques et conscients de l'interaction. Enfin, nous explorons le conditionnement de structure dans "Uncovering Sequence Diversity from a Known Protein Structure". Ici, nous présentons InvMSAFold, une méthode qui produit des séquences de protéines diverses conçues pour se plier dans une structure spécifique. Cette approche met en lumière l'importance de considérer la structure finale de la protéine dans le processus de conception, permettant la génération de séquences qui sont non seulement diverses mais maintiennent également leur intégrité structurelle prévue
This thesis explores the intersection of artificial intelligence (AI) and biology, focusing on how generative models can innovate in protein sequence design. Our research unfolds in three distinct yet interconnected stages, each building upon the insights of the previous to enhance the model's applicability and performance in protein engineering.We begin by examining what makes a generative model effective for protein sequences. In our first study, "Interpretable Pairwise Distillations for Generative Protein Sequence Models," we compare complex neural network models to simpler, pairwise distribution models. This comparison highlights that deep learning strategy mainly model second order interaction, highlighting their fundamental role in modeling proteins family.In a second part, we try to expand this principle of using second order interaction to inverse folding. We explore structure conditioning in "Uncovering Sequence Diversity from a Known Protein Structure" Here, we present InvMSAFold, a method that produces diverse protein sequences designed to fold into a specific structure. This approach tries to combines two different tradition of proteins modeling: the MSA based models that try to capture the entire fitness landscape and the inverse folding types of model that focus on recovering one specific sequence. This is a first step towards the possibility of conditioning the fitness landscape by considering the protein's final structure in the design process, enabling the generation of sequences that are not only diverse but also maintain their intended structural integrity. Finally, we delve into sequence conditioning with "Generating Interacting Protein Sequences using Domain-to-Domain Translation." This study introduces a novel approach to generate protein sequences that can interact with specific other proteins. By treating this as a translation problem, similar to methods used in language processing, we create sequences with intended functionalities. Furthermore, we address the critical challenge of T-cell receptor (TCR) and epitope interaction prediction in "TULIP—a Transformer based Unsupervised Language model for Interacting Peptides and T-cell receptors." This study introduces an unsupervised learning approach to accurately predict TCR-epitope bindings, overcoming limitations in data quality and training bias inherent in previous models. These advancements underline the potential of sequence conditioning in creating functionally specific and interaction-aware protein designs

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Vander, Meersche Yann. "Étude de la flexibilité des protéines : analyse à grande échelle de simulations de dynamique moléculaire et prédiction par apprentissage profond". Electronic Thesis or Diss., Université Paris Cité, 2024. http://www.theses.fr/2024UNIP5147.

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Les protéines sont essentielles aux processus biologiques. Comprendre leur dynamique est crucial pour élucider leurs fonctions biologiques et leurs interactions. Cependant, mesurer expérimentalement cette flexibilité reste un défi en raison des limitations techniques et des coûts associés. Cette thèse vise à approfondir la compréhension des propriétés dynamiques des protéines et à proposer des méthodes bioinformatique permettant de prédire leur flexibilité directement à partir de leur séquence. Ces travaux s'organisent en quatre axes principaux : 1) Prédiction de la flexibilité des protéines en termes de B-facteurs. Nous avons développé MEDUSA, une méthode de prédiction de la flexibilité basée sur l'apprentissage profond, qui exploite les informations physico-chimiques et évolutives des acides aminés pour prédire des classes de flexibilité expérimentale à partir des séquences protéiques. MEDUSA s'est révélé plus performant que les outils précédemment disponibles, mais présente des limitations en raison de la variabilité des données expérimentales. 2) Analyse à grande échelle de la dynamique protéique in silico. Nous avons mis à disposition ATLAS, une base de données de simulations de dynamique moléculaire tout-atome standardisées, fournissant des informations détaillées sur la flexibilité des protéines pour plus de 1500 structures représentatives. ATLAS permet une analyse interactive de la dynamique des protéines à différents niveaux et offre des informations précieuses sur les protéines présentant des comportements dynamiques atypiques, tels que les fragments à double personnalité. 3) Analyse approfondie du score pLDDT d'AlphaFold 2 et sa relation avec la flexibilité des protéines. Nous avons évalué la corrélation du pLDDT avec différents descripteurs de flexibilité dérivés de simulations de dynamique moléculaire et d'ensembles RMN, et démontré que la confiance dans la prédiction de la structure 3D ne reflète pas nécessairement la flexibilité attendue de la région protéique, en particulier pour les fragments protéiques impliqués dans des interactions moléculaires. 4) Prédiction des descripteurs de flexibilité dérivés de la dynamique moléculaire à partir des embeddings de langages protéiques. Nous présentons PEGASUS, un nouvel outil de prédiction de la flexibilité développé à partir de la base de données ATLAS. En utilisant l'encodage des séquences protéiques par des modèles de langage protéique et un modèle d'apprentissage profond simple, PEGASUS fournit des prédictions précises des métriques de flexibilité et capture efficacement l'impact des mutations sur la dynamique des protéines. Les perspectives de ce travail incluent l'enrichissement des simulations avec des environnements variés et l'intégration des protéines membranaires pour améliorer PEGASUS et réaliser de nouvelles analyses. Nous évoquons également l'émergence des méthodes capables de prédire des ensembles conformationnels, offrant des avancées prometteuses pour mieux capturer la dynamique des protéines. Cette thèse ouvre de nouvelles perspectives pour la prédiction et l'analyse de la flexibilité des protéines, ouvrant la voie à des avancées dans des domaines tels que la recherche biomédicale, l'étude des mutations et la conception de médicaments
Proteins are essential to biological processes. Understanding their dynamics is crucial for elucidating their biological functions and interactions. However, experimentally measuring protein flexibility remains challenging due to technical limitations and associated costs. This thesis aims to deepen the understanding of protein dynamic properties and to propose computational methods for predicting their flexibility directly from their sequence. This work is organised in four main contributions: 1) Protein flexibility prediction in terms of B-factors. We have developed MEDUSA, a flexibility prediction method based on deep learning, which leverages the physicochemical and evolutionary information of amino acids to predict experimental flexibility classes from protein sequences. MEDUSA has outperformed previously available tools but shows limitations due to the variability of experimental data. 2) Large-scale analysis of in silico protein dynamics. We have released ATLAS, a database of standardised all-atom molecular dynamics simulations providing detailed information on protein flexibility for over 1.5k representative protein structures. ATLAS enables interactive analysis of protein dynamics at different levels and offers valuable insights into proteins exhibiting atypical dynamical behaviour, such as dual personality fragments. 3) An in-depth analysis of AlphaFold 2's pLDDT score and its relation to protein flexibility. We have assessed pLDDT correlation with different flexibility descriptors derived from molecular dynamics simulations and from NMR ensembles and demonstrated that confidence in 3D structure prediction does not necessarily reflect expected flexibility of the protein region, in particular, for protein fragments involved in molecular interaction. 4) Prediction of MD-derived flexibility descriptors using protein language embeddings. We introduce PEGASUS, a novel flexibility prediction tool developed using ATLAS database. Using protein sequence encoding by protein language models and a simple deep learning model, PEGASUS provides precise predictions of flexibility metrics and effectively captures the impact of mutations on protein dynamics. The perspectives of this work include enriching simulations with varied environments and integrating membrane proteins to enhance PEGASUS and enable new analyses. We also highlight the emergence of methods capable of predicting conformational ensembles, offering promising advances for better capturing protein dynamics. This thesis offers new perspectives for the prediction and analysis of protein flexibility, paving the way for advances in areas such as biomedical research, mutation studies, and drug design

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Hladiš, Matej. "Réseaux de neurones en graphes et modèle de langage des protéines pour révéler le code combinatoire de l'olfaction". Electronic Thesis or Diss., Université Côte d'Azur, 2024. http://www.theses.fr/2024COAZ5024.

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Les mammifères identifient et interprètent une myriade de stimuli olfactifs par un mécanisme de codage complexe reposant sur la reconnaissance des molécules odorantes par des centaines de récepteurs olfactifs (RO). Ces interactions génèrent des combinaisons uniques de récepteurs activés, appelées code combinatoire, que le cerveau humain interprète comme la sensation que nous appelons l'odeur. Jusqu'à présent, le grand nombre de combinaisons possibles entre les récepteurs et les molécules a empêché une étude expérimentale à grande échelle de ce code et de son lien avec la perception des odeurs. La révélation de ce code est donc cruciale pour répondre à la question à long terme de savoir comment nous percevons notre environnement chimique complexe. Les RO appartiennent à la classe A des récepteurs couplés aux protéines G (RCPG) et constituent la plus grande famille multigénique connue. Pour étudier de façon systématique le codage olfactif, nous avons développé M2OR, une base de données exhaustive compilant les 25 dernières années d'essais biologiques sur les RO. À l'aide de cet ensemble de données, un modèle d'apprentissage profond sur mesure a été conçu et entraîné. Il combine l'intégration de jetons [CLS] d'un modèle de langage des protéines avec des réseaux de neurones en graphes et un mécanisme d'attention multi-têtes. Ce modèle prédit l'activation des RO par les odorants et révèle le code combinatoire résultant pour toute molécule odorante. Cette approche est affinée en développant un nouveau modèle capable de prédire l'activité d'un odorant à une concentration spécifique, permettant alors d'estimer la valeur d'EC50 de n'importe quelle paire OR-odorant. Enfin, les codes combinatoires dérivés des deux modèles sont utilisés pour prédire la perception olfactive des molécules. En incorporant des biais inductifs inspirés par la théorie du codage olfactif, un modèle d'apprentissage automatique basé sur ces codes est plus performant que l'état de l'art actuel en matière de prédiction d'odeurs. À notre connaissance, il s'agit de l'application la plus aboutie liant le code combinatoire à la prédiction de l'odeur d'une molécule. Dans l'ensemble, ce travail établit un lien entre les interactions complexes molécule odorante-récepteur et la perception humaine
Mammals identify and interpret a myriad of olfactory stimuli using a complex coding mechanism involving interactions between odorant molecules and hundreds of olfactory receptors (ORs). These interactions generate unique combinations of activated receptors, called the combinatorial code, which the human brain interprets as the sensation we call smell. Until now, the vast number of possible receptor-molecule combinations have prevented a large-scale experimental study of this code and its link to odor perception. Therefore, revealing this code is crucial to answering the long-term question of how we perceive our intricate chemical environment. ORs belong to the class A of G protein-coupled receptors (GPCRs) and constitute the largest known multigene family. To systematically study olfactory coding, we develop M2OR, a comprehensive database compiling the last 25 years of OR bioassays. Using this dataset, a tailored deep learning model is designed and trained. It combines the [CLS] token embedding from a protein language model with graph neural networks and multi-head attention. This model predicts the activation of ORs by odorants and reveals the resulting combinatorial code for any odorous molecule. This approach is refined by developing a novel model capable of predicting the activity of an odorant at a specific concentration, subsequently allowing the estimation of the EC50 value for any OR-odorant pair. Finally, the combinatorial codes derived from both models are used to predict the odor perception of molecules. By incorporating inductive biases inspired by olfactory coding theory, a machine learning model based on these codes outperforms the current state-of-the-art in smell prediction. To the best of our knowledge, this is the most comprehensive and successful application of combinatorial coding to odor quality prediction. Overall, this work provides a link between the complex molecule-receptor interactions and human perception

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Książki na temat "Protein language models"

Bovington, Sue. Tigris/Thames. [United Kingdom]: [Sue Bovington], 2011.

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Beatriz, Solís Leree, red. La Ley televisa y la lucha por el poder en México. México, D.F: Universidad Autónoma Metropolitana, Unidad Xochimilco, 2009.

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Yoshikawa, Saeko. William Wordsworth and Modern Travel. Liverpool University Press, 2020. http://dx.doi.org/10.3828/liverpool/9781789621181.001.0001.

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This book explores William Wordsworth’s pervasive influence on the tourist landscapes of the Lake District throughout the age of transport revolutions, popular tourism, and the Great 1914-18 War. It reveals how Wordsworth’s response to railways was not a straightforward matter of opposition and protest; his ideas were taken up by advocates and opponents of railways, and through their controversies had a surprising impact on the earliest motorists as they sought a language to describe the liberty and independence of their new mode of travel. Once the age of motoring was underway, the outbreak of the First World War encouraged British people to connect Wordsworth’s patriotic passion with his wish to protect the Lake District as a national heritage—a transition that would have momentous effects in the interwar period when the popularisation of motoring paradoxically brought a vogue for open-air activities and a renewal of Romantic pedestrianism. With the arrival of global tourism, preservation of the cultural landscape of the Lake District became an urgent national and international concern. By revealing how Romantic ideas of nature, travel, liberty and self-reliance were re-interpreted and utilized in discourses on landscape, transport, accessibility, preservation, war and cultural heritage, this book portrays multiple Wordsworthian legacies in modern ways of perceiving and valuing the nature and culture of the Lake District.

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Hardiman, David. The Nonviolent Struggle for Indian Freedom, 1905-19. Oxford University Press, 2018. http://dx.doi.org/10.1093/oso/9780190920678.001.0001.

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Much of the recent surge in writing about the practice of nonviolent forms of resistance has focused on movements that occurred after the end of the Second World War, many of which have been extremely successful. Although the fact that such a method of civil resistance was developed in its modern form by Indians is acknowledged in this writing, there has not until now been an authoritative history of the role of Indians in the evolution of the phenomenon.The book argues that while nonviolence is associated above all with the towering figure of Mahatma Gandhi, 'passive resistance' was already being practiced as a form of civil protest by nationalists in British-ruled India, though there was no principled commitment to nonviolence as such. The emphasis was on efficacy, rather than the ethics of such protest. It was Gandhi, first in South Africa and then in India, who evolved a technique that he called 'satyagraha'. He envisaged this as primarily a moral stance, though it had a highly practical impact. From 1915 onwards, he sought to root his practice in terms of the concept of ahimsa, a Sanskrit term that he translated as ‘nonviolence’. His endeavors saw 'nonviolence' forged as both a new word in the English language, and as a new political concept. This book conveys in vivid detail exactly what such nonviolence entailed, and the formidable difficulties that the pioneers of such resistance encountered in the years 1905-19.

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McNally, Michael D. Defend the Sacred. Princeton University Press, 2020. http://dx.doi.org/10.23943/princeton/9780691190907.001.0001.

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From North Dakota's Standing Rock encampments to Arizona's San Francisco Peaks, Native Americans have repeatedly asserted legal rights to religious freedom to protect their sacred places, practices, objects, knowledge, and ancestral remains. But these claims have met with little success in court because Native American communal traditions don't fit easily into modern Western definitions of religion. This book explores how, in response to this situation, Native peoples have creatively turned to other legal means to safeguard what matters to them. To articulate their claims, Native peoples have resourcefully used the languages of cultural resources under environmental and historic preservation law; of sovereignty under treaty-based federal Indian law; and, increasingly, of Indigenous rights under international human rights law. Along the way, Native nations still draw on the rhetorical power of religious freedom to gain legislative and regulatory successes beyond the First Amendment. This book casts new light on discussions of religious freedom, cultural resource management, and the vitality of Indigenous religions today.

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Meddings, Jennifer, Vineet Chopra i Sanjay Saint. Preventing Hospital Infections. Wyd. 2. Oxford University Press, 2021. http://dx.doi.org/10.1093/med/9780197509159.001.0001.

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This book provides a detailed, step-by-step description of a model quality improvement intervention for hospitals, pinpointing the obstacles and showing how to surmount them. This second edition has been carefully updated, with new material describing some technical aspects of infection prevention, new tools for use by front-line providers, and results of recent large collaborative infection prevention studies. In easy-to-read, user-friendly language, it explains why clinicians neglect or actively oppose quality changes—from physicians who distrust change, to nurses who want to protect their turf, to infection preventionists who avoid the wards. The book also sheds light on how and why hospitals embark on quality improvements, the role of the hospital’s leadership cadre, the selection and training of the project team, and how to sustain quality gains long term. The intervention framework described in the book focuses on the prevention of hospital-associated infections—in particular, catheter-associated urinary tract infection (CAUTI)—but it is directly applicable to a variety of other hospital issues, such as falls, pressure sores, and Clostridioides difficile infection (CDI). In fact, the book includes a chapter applying this framework to a CDI prevention initiative. In addition, for hospitals having trouble with staff adherence to a quality initiative, we provide three infection-specific questionnaires (for CAUTI, CLABSI, and CDI) to help pinpoint individual problems, and provide a link to a website offering advice tailored to their specific circumstances.

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Halvorsen, Tar, i Peter Vale. One World, Many Knowledges: Regional experiences and cross-regional links in higher education. African Minds, 2016. http://dx.doi.org/10.47622/978-0-620-55789-4.

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Various forms of academic co-operation criss-cross the modern university system in a bewildering number of ways, from the open exchange of ideas and knowledge, to the sharing of research results, and frank discussions about research challenges. Embedded in these scholarly networks is the question of whether a global template for the management of both higher education and national research organisations is necessary, and if so, must institutions slavishly follow the high-flown language of the global knowledge society or risk falling behind in the ubiquitous university ranking system? Or are there alternatives that can achieve a better, more ethically inclined, world? Basing their observations on their own experiences, an interesting mix of seasoned scholars and new voices from southern Africa and the Nordic region offer critical perspectives on issues of inter- and cross-regional academic co-operation. Several of the chapters also touch on the evolution of the higher education sector in the two regions. An absorbing and intelligent study, this book will be invaluable for anyone interested in the strategies scholars are using to adapt to the interconnectedness of the modern world. It offers fresh insights into how academics are attempting to protect the spaces in which they can freely and openly debate the challenges they face, while aiming to transform higher education, and foster scholarly collaboration. The Southern African-Nordic Centre (SANORD) is a partnership of higher education institutions from Denmark, Finland, Iceland, Norway, Sweden, Botswana, Namibia, Malawi, South Africa, Zambia and Zimbabwe. SANORDs primary aim is to promote multilateral research co-operation on matters of importance to the development of both regions. Our activities are based on the values of democracy, equity, and mutually beneficial academic engagement.

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Części książek na temat "Protein language models"

Xu, Yaoyao, Xinjian Zhao, Xiaozhuang Song, Benyou Wang i Tianshu Yu. "Boosting Protein Language Models with Negative Sample Mining". W Lecture Notes in Computer Science, 199–214. Cham: Springer Nature Switzerland, 2024. http://dx.doi.org/10.1007/978-3-031-70381-2_13.

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Zhao, Junming, Chao Zhang i Yunan Luo. "Contrastive Fitness Learning: Reprogramming Protein Language Models for Low-N Learning of Protein Fitness Landscape". W Lecture Notes in Computer Science, 470–74. Cham: Springer Nature Switzerland, 2024. http://dx.doi.org/10.1007/978-1-0716-3989-4_55.

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Pratyush, Pawel, Suresh Pokharel, Hamid D. Ismail, Soufia Bahmani i Dukka B. KC. "LMPTMSite: A Platform for PTM Site Prediction in Proteins Leveraging Transformer-Based Protein Language Models". W Methods in Molecular Biology, 261–97. New York, NY: Springer US, 2024. http://dx.doi.org/10.1007/978-1-0716-4196-5_16.

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Ghazikhani, Hamed, i Gregory Butler. "A Study on the Application of Protein Language Models in the Analysis of Membrane Proteins". W Distributed Computing and Artificial Intelligence, Special Sessions, 19th International Conference, 147–52. Cham: Springer International Publishing, 2023. http://dx.doi.org/10.1007/978-3-031-23210-7_14.

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Wu, Tianqi, Weihang Cheng i Jianlin Cheng. "Improving Protein Secondary Structure Prediction by Deep Language Models and Transformer Networks". W Methods in Molecular Biology, 43–53. New York, NY: Springer US, 2024. http://dx.doi.org/10.1007/978-1-0716-4196-5_3.

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Zeng, Shuai, Duolin Wang, Lei Jiang i Dong Xu. "Prompt-Based Learning on Large Protein Language Models Improves Signal Peptide Prediction". W Lecture Notes in Computer Science, 400–405. Cham: Springer Nature Switzerland, 2024. http://dx.doi.org/10.1007/978-1-0716-3989-4_40.

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Fernández, Diego, Álvaro Olivera-Nappa, Roberto Uribe-Paredes i David Medina-Ortiz. "Exploring Machine Learning Algorithms and Protein Language Models Strategies to Develop Enzyme Classification Systems". W Bioinformatics and Biomedical Engineering, 307–19. Cham: Springer Nature Switzerland, 2023. http://dx.doi.org/10.1007/978-3-031-34953-9_24.

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Paaß, Gerhard, i Sven Giesselbach. "Foundation Models for Speech, Images, Videos, and Control". W Artificial Intelligence: Foundations, Theory, and Algorithms, 313–82. Cham: Springer International Publishing, 2023. http://dx.doi.org/10.1007/978-3-031-23190-2_7.

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AbstractFoundation Models are able to model not only tokens of natural language but also token elements of arbitrary sequences. For images, square image patches can be represented as tokens; for videos, we can define tubelets that span an image patch across multiple frames. Subsequently, the proven self-attention algorithms can be applied to these tokens. Most importantly, several modalities like text and images can be processed in the same sequence allowing, for instance, the generation of images from text and text descriptions from video. In addition, the models are scalable to very large networks and huge datasets. The following multimedia types are covered in the subsequent sections. Speech recognition and text-to-speech models describe the translation of spoken language into text and vice versa. Image processing has the task to interpret images, describe them by captions, and generate new images according to textual descriptions. Video interpretation aims at recognizing action in videos and describing them through text. Furthermore, new videos can be created according to a textual description. Dynamical system trajectories characterize sequential decision problems, which can be simulated and controlled. DNA and protein sequences can be analyzed with Foundation Models to predict the structure and properties of the corresponding molecules.

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Shan, Kaixuan, Xiankun Zhang i Chen Song. "Prediction of Protein-DNA Binding Sites Based on Protein Language Model and Deep Learning". W Advanced Intelligent Computing in Bioinformatics, 314–25. Singapore: Springer Nature Singapore, 2024. http://dx.doi.org/10.1007/978-981-97-5692-6_28.

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Matsiunova, Antonina. "Semantic opposition of US versus THEM in late 2020 Russian-language Belarusian discourse". W Protest in Late Modern Societies, 42–55. London: Routledge, 2023. http://dx.doi.org/10.4324/9781003270065-4.

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Streszczenia konferencji na temat "Protein language models"

Amjad, Maheera, Ayesha Munir, Usman Zia i Rehan Zafar Paracha. "Pre-trained Language Models for Decoding Protein Language: a Survey". W 2024 4th International Conference on Digital Futures and Transformative Technologies (ICoDT2), 1–12. IEEE, 2024. http://dx.doi.org/10.1109/icodt262145.2024.10740205.

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Liu, Xu, Yiming Li, Fuhao Zhang, Ruiqing Zheng, Fei Guo, Min Li i Min Zeng. "ComLMEss: Combining multiple protein language models enables accurate essential protein prediction". W 2024 IEEE International Conference on Bioinformatics and Biomedicine (BIBM), 67–72. IEEE, 2024. https://doi.org/10.1109/bibm62325.2024.10822205.

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Sun, Xin, i Yuhao Wu. "Combinative Bio-feature Proteins Generation via Pre-trained Protein Large Language Models". W 2024 IEEE International Conference on Bioinformatics and Biomedicine (BIBM), 99–104. IEEE, 2024. https://doi.org/10.1109/bibm62325.2024.10821839.

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Liang, Po-Yu, Xueting Huang, Tibo Duran, Andrew J. Wiemer i Jun Bai. "Exploring Latent Space for Generating Peptide Analogs Using Protein Language Models". W 2024 IEEE International Conference on Bioinformatics and Biomedicine (BIBM), 842–47. IEEE, 2024. https://doi.org/10.1109/bibm62325.2024.10821777.

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Jiang, Yanfeng, Ning Sun, Zhengxian Lu, Shuang Peng, Yi Zhang, Fei Yang i Tao Li. "MEFold: Memory-Efficient Optimization for Protein Language Models via Chunk and Quantization". W 2024 International Joint Conference on Neural Networks (IJCNN), 1–8. IEEE, 2024. http://dx.doi.org/10.1109/ijcnn60899.2024.10651470.

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Kim, Yunsoo. "Foundation Model for Biomedical Graphs: Integrating Knowledge Graphs and Protein Structures to Large Language Models". W Proceedings of the 62nd Annual Meeting of the Association for Computational Linguistics (Volume 4: Student Research Workshop), 346–55. Stroudsburg, PA, USA: Association for Computational Linguistics, 2024. http://dx.doi.org/10.18653/v1/2024.acl-srw.30.

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Shen, Yiqing, Zan Chen, Michail Mamalakis, Luhan He, Haiyang Xia, Tianbin Li, Yanzhou Su, Junjun He i Yu Guang Wang. "A Fine-tuning Dataset and Benchmark for Large Language Models for Protein Understanding". W 2024 IEEE International Conference on Bioinformatics and Biomedicine (BIBM), 2390–95. IEEE, 2024. https://doi.org/10.1109/bibm62325.2024.10821894.

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Zhang, Jun, Zhiqiang Yan, Hao Zeng i Zexuan Zhu. "PAIR: protein-aptamer interaction prediction based on language models and contrastive learning framework". W 2024 IEEE International Conference on Bioinformatics and Biomedicine (BIBM), 5426–32. IEEE, 2024. https://doi.org/10.1109/bibm62325.2024.10822859.

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Engel, Ryan, i Gilchan Park. "Evaluating Large Language Models for Predicting Protein Behavior under Radiation Exposure and Disease Conditions". W Proceedings of the 23rd Workshop on Biomedical Natural Language Processing, 427–39. Stroudsburg, PA, USA: Association for Computational Linguistics, 2024. http://dx.doi.org/10.18653/v1/2024.bionlp-1.34.

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Tsutaoka, Takuya, Noriji Kato, Toru Nishino, Yuanzhong Li i Masahito Ohue. "Predicting Antibody Stability pH Values from Amino Acid Sequences: Leveraging Protein Language Models for Formulation Optimization". W 2024 IEEE International Conference on Bioinformatics and Biomedicine (BIBM), 240–43. IEEE, 2024. https://doi.org/10.1109/bibm62325.2024.10822009.

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Raporty organizacyjne na temat "Protein language models"

Wu, Jyun-Jie. Improving Predictive Efficiency and Literature Quality Assessment for Lung Cancer Complications Post-Proton Therapy Through Large Language Models and Meta-Analysis. INPLASY - International Platform of Registered Systematic Review and Meta-analysis Protocols, sierpień 2024. http://dx.doi.org/10.37766/inplasy2024.8.0103.

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Shani, Uri, Lynn Dudley, Alon Ben-Gal, Menachem Moshelion i Yajun Wu. Root Conductance, Root-soil Interface Water Potential, Water and Ion Channel Function, and Tissue Expression Profile as Affected by Environmental Conditions. United States Department of Agriculture, październik 2007. http://dx.doi.org/10.32747/2007.7592119.bard.

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Constraints on water resources and the environment necessitate more efficient use of water. The key to efficient management is an understanding of the physical and physiological processes occurring in the soil-root hydraulic continuum.While both soil and plant leaf water potentials are well understood, modeled and measured, the root-soil interface where actual uptake processes occur has not been sufficiently studied. The water potential at the root-soil interface (yᵣₒₒₜ), determined by environmental conditions and by soil and plant hydraulic properties, serves as a boundary value in soil and plant uptake equations. In this work, we propose to 1) refine and implement a method for measuring yᵣₒₒₜ; 2) measure yᵣₒₒₜ, water uptake and root hydraulic conductivity for wild type tomato and Arabidopsis under varied q, K⁺, Na⁺ and Cl⁻ levels in the root zone; 3) verify the role of MIPs and ion channels response to q, K⁺ and Na⁺ levels in Arabidopsis and tomato; 4) study the relationships between yᵣₒₒₜ and root hydraulic conductivity for various crops representing important botanical and agricultural species, under conditions of varying soil types, water contents and salinity; and 5) integrate the above to water uptake term(s) to be implemented in models. We have made significant progress toward establishing the efficacy of the emittensiometer and on the molecular biology studies. We have added an additional method for measuring ψᵣₒₒₜ. High-frequency water application through the water source while the plant emerges and becomes established encourages roots to develop towards and into the water source itself. The yᵣₒₒₜ and yₛₒᵢₗ values reflected wetting and drying processes in the rhizosphere and in the bulk soil. Thus, yᵣₒₒₜ can be manipulated by changing irrigation level and frequency. An important and surprising finding resulting from the current research is the obtained yᵣₒₒₜ value. The yᵣₒₒₜ measured using the three different methods: emittensiometer, micro-tensiometer and MRI imaging in both sunflower, tomato and corn plants fell in the same range and were higher by one to three orders of magnitude from the values of -600 to -15,000 cm suggested in the literature. We have added additional information on the regulation of aquaporins and transporters at the transcript and protein levels, particularly under stress. Our preliminary results show that overexpression of one aquaporin gene in tomato dramatically increases its transpiration level (unpublished results). Based on this information, we started screening mutants for other aquaporin genes. During the feasibility testing year, we identified homozygous mutants for eight aquaporin genes, including six mutants for five of the PIP2 genes. Including the homozygous mutants directly available at the ABRC seed stock center, we now have mutants for 11 of the 19 aquaporin genes of interest. Currently, we are screening mutants for other aquaporin genes and ion transporter genes. Understanding plant water uptake under stress is essential for the further advancement of molecular plant stress tolerance work as well as for efficient use of water in agriculture. Virtually all of Israel’s agriculture and about 40% of US agriculture is made possible by irrigation. Both countries face increasing risk of water shortages as urban requirements grow. Both countries will have to find methods of protecting the soil resource while conserving water resources—goals that appear to be in direct conflict. The climate-plant-soil-water system is nonlinear with many feedback mechanisms. Conceptual plant uptake and growth models and mechanism-based computer-simulation models will be valuable tools in developing irrigation regimes and methods that maximize the efficiency of agricultural water. This proposal will contribute to the development of these models by providing critical information on water extraction by the plant that will result in improved predictions of both water requirements and crop yields. Plant water use and plant response to environmental conditions cannot possibly be understood by using the tools and language of a single scientific discipline. This proposal links the disciplines of soil physics and soil physical chemistry with plant physiology and molecular biology in order to correctly treat and understand the soil-plant interface in terms of integrated comprehension. Results from the project will contribute to a mechanistic understanding of the SPAC and will inspire continued multidisciplinary research.

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Melnyk, Iurii. JUSTIFICATION OF OCCUPATION IN GERMAN (1938) AND RUSSIAN (2014) MEDIA: SUBSTITUTION OF AGGRESSOR AND VICTIM. Ivan Franko National University of Lviv, marzec 2021. http://dx.doi.org/10.30970/vjo.2021.50.11101.

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The article is dedicated to the examination and comparison of the justification of occupation of a neighboring country in the German (1938) and Russian (2014) media. The objective of the study is to reveal the mechanics of the application of the classical manipulative method of substituting of aggressor and victim on the material of German and Russian propaganda in 1938 and in 2014 respectively. According to the results of the study, clear parallels between the two information strategies can be traced at the level of the condemnation of internal aggression against a national minority loyal to Berlin / Moscow and its political representative (the Sudeten Germans – the pro-Russian Ukrainians, as well as the security forces of the Yanukovych regime); the reflections on dangers that Czechoslovakia / Ukraine poses to itself and to its neighbors; condemnation of the violation of the cultural rights of the minority that the occupier intends to protect (German language and culture – Russian language and culture); the historical parallels designed to deepen the modern conflict, to show it as a long-standing and a natural one (“Hussites” – “Banderites”). In the manipulative strategy of both media, the main focus is not on factual fabrication, but on the bias selection of facts, due to which the reader should have an unambiguous understanding of who is the permanent aggressor in the conflict (Czechoslovakia, Czechs – Ukraine, Ukrainians), and who is the permanent victim (Germans – Russians, Russian speakers). The substitution of victim and aggressor in the media in both cases became one of the most important manipulative strategies designed to justify the German occupation of part of Czechoslovakia and the Russian occupation of part of Ukraine.

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Yatsymirska, Mariya. KEY IMPRESSIONS OF 2020 IN JOURNALISTIC TEXTS. Ivan Franko National University of Lviv, marzec 2021. http://dx.doi.org/10.30970/vjo.2021.50.11107.

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The article explores the key vocabulary of 2020 in the network space of Ukraine. Texts of journalistic, official-business style, analytical publications of well-known journalists on current topics are analyzed. Extralinguistic factors of new word formation, their adaptation to the sphere of special and socio-political vocabulary of the Ukrainian language are determined. Examples show modern impressions in the media, their stylistic use and impact on public opinion in a pandemic. New meanings of foreign expressions, media terminology, peculiarities of translation of neologisms from English into Ukrainian have been clarified. According to the materials of the online media, a «dictionary of the coronavirus era» is provided. The journalistic text functions in the media on the basis of logical judgments, credible arguments, impressive language. Its purpose is to show the socio-political problem, to sharpen its significance for society and to propose solutions through convincing considerations. Most researchers emphasize the influential role of journalistic style, which through the media shapes public opinion on issues of politics, economics, education, health care, war, the future of the country. To cover such a wide range of topics, socio-political vocabulary is used first of all – neutral and emotionally-evaluative, rhetorical questions and imperatives, special terminology, foreign words. There is an ongoing discussion in online publications about the use of the new foreign token «lockdown» instead of the word «quarantine», which has long been learned in the Ukrainian language. Research on this topic has shown that at the initial stage of the pandemic, the word «lockdown» prevailed in the colloquial language of politicians, media personalities and part of society did not quite understand its meaning. Lockdown, in its current interpretation, is a restrictive measure to protect people from a dangerous virus that has spread to many countries; isolation of the population («stay in place») in case of risk of spreading Covid-19. In English, US citizens are told what a lockdown is: «A lockdown is a restriction policy for people or communities to stay where they are, usually due to specific risks to themselves or to others if they can move and interact freely. The term «stay-at-home» or «shelter-in-place» is often used for lockdowns that affect an area, rather than specific locations». Content analysis of online texts leads to the conclusion that in 2020 a special vocabulary was actively functioning, with the appropriate definitions, which the media described as a «dictionary of coronavirus vocabulary». Media broadcasting is the deepest and pulsating source of creative texts with new meanings, phrases, expressiveness. The influential power of the word finds its unconditional embodiment in the media. Journalists, bloggers, experts, politicians, analyzing current events, produce concepts of a new reality. The world is changing and the language of the media is responding to these changes. It manifests itself most vividly and emotionally in the network sphere, in various genres and styles.

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Or, Etti, David Galbraith i Anne Fennell. Exploring mechanisms involved in grape bud dormancy: Large-scale analysis of expression reprogramming following controlled dormancy induction and dormancy release. United States Department of Agriculture, grudzień 2002. http://dx.doi.org/10.32747/2002.7587232.bard.

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The timing of dormancy induction and release is very important to the economic production of table grape. Advances in manipulation of dormancy induction and dormancy release are dependent on the establishment of a comprehensive understanding of biological mechanisms involved in bud dormancy. To gain insight into these mechanisms we initiated the research that had two main objectives: A. Analyzing the expression profiles of large subsets of genes, following controlled dormancy induction and dormancy release, and assessing the role of known metabolic pathways, known regulatory genes and novel sequences involved in these processes B. Comparing expression profiles following the perception of various artificial as well as natural signals known to induce dormancy release, and searching for gene showing similar expression patterns, as candidates for further study of pathways having potential to play a central role in dormancy release. We first created targeted EST collections from V. vinifera and V. riparia mature buds. Clones were randomly selected from cDNA libraries prepared following controlled dormancy release and controlled dormancy induction and from respective controls. The entire collection (7920 vinifera and 1194 riparia clones) was sequenced and subjected to bioinformatics analysis, including clustering, annotations and GO classifications. PCR products from the entire collection were used for printing of cDNA microarrays. Bud tissue in general, and the dormant bud in particular, are under-represented within the grape EST database. Accordingly, 59% of the our vinifera EST collection, composed of 5516 unigenes, are not included within the current Vitis TIGR collection and about 22% of these transcripts bear no resemblance to any known plant transcript, corroborating the current need for our targeted EST collection and the bud specific cDNA array. Analysis of the V. riparia sequences yielded 814 unigenes, of which 140 are unique (keilin et al., manuscript, Appendix B). Results from computational expression profiling of the vinifera collection suggest that oxidative stress, calcium signaling, intracellular vesicle trafficking and anaerobic mode of carbohydrate metabolism play a role in the regulation and execution of grape-bud dormancy release. A comprehensive analysis confirmed the induction of transcription from several calcium–signaling related genes following HC treatment, and detected an inhibiting effect of calcium channel blocker and calcium chelator on HC-induced and chilling-induced bud break. It also detected the existence of HC-induced and calcium dependent protein phosphorylation activity. These data suggest, for the first time, that calcium signaling is involved in the mechanism of dormancy release (Pang et al., in preparation). We compared the effects of heat shock (HS) to those detected in buds following HC application and found that HS lead to earlier and higher bud break. We also demonstrated similar temporary reduction in catalase expression and temporary induction of ascorbate peroxidase, glutathione reductase, thioredoxin and glutathione S transferase expression following both treatments. These findings further support the assumption that temporary oxidative stress is part of the mechanism leading to bud break. The temporary induction of sucrose syntase, pyruvate decarboxylase and alcohol dehydrogenase indicate that temporary respiratory stress is developed and suggest that mitochondrial function may be of central importance for that mechanism. These finding, suggesting triggering of identical mechanisms by HS and HC, justified the comparison of expression profiles of HC and HS treated buds, as a tool for the identification of pathways with a central role in dormancy release (Halaly et al., in preparation). RNA samples from buds treated with HS, HC and water were hybridized with the cDNA arrays in an interconnected loop design. Differentially expressed genes from the were selected using R-language package from Bioconductor project called LIMMA and clones showing a significant change following both HS and HC treatments, compared to control, were selected for further analysis. A total of 1541 clones show significant induction, of which 37% have no hit or unknown function and the rest represent 661 genes with identified function. Similarly, out of 1452 clones showing significant reduction, only 53% of the clones have identified function and they represent 573 genes. The 661 induced genes are involved in 445 different molecular functions. About 90% of those functions were classified to 20 categories based on careful survey of the literature. Among other things, it appears that carbohydrate metabolism and mitochondrial function may be of central importance in the mechanism of dormancy release and studies in this direction are ongoing. Analysis of the reduced function is ongoing (Appendix A). A second set of hybridizations was carried out with RNA samples from buds exposed to short photoperiod, leading to induction of bud dormancy, and long photoperiod treatment, as control. Analysis indicated that 42 genes were significant difference between LD and SD and 11 of these were unique.

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