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1

Polák, J., M. Ravelonandro, J. Kumar-Kundu, J. Pívalová i R. Scorza. "Interactions of Plum pox virus strain Rec with Apple chlorotic leafspot virus and Prune dwarf viruses in field-grown transgenic plum Prunus domestica L., clone C5". Plant Protection Science 44, No. 1 (10.04.2008): 1–5. http://dx.doi.org/10.17221/535-pps.

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Transgenic plums, <I>Prunus domestica</I> L. clone C5, were inoculated by bud grafting with <I>Plum pox virus</I> (PPV-Rec, recombinant strain originated from plum), PPV-Rec + <I>Apple chlorotic leafspot virus</I> (ACLSV), PPV-Rec + <I>Prune dwarf virus</I> (PDV), and PPV-Rec + ACLSV + PDV. Non-inoculated transgenic plums served as controls. Plants were grown in an open field for 5 years. They were evaluated by visible symptoms, by DAS-ELISA and RT-PCR. Mild PPV symptoms, diffuse spots or rings appeared two years after inoculation in some leaves of plants artificially inoculated with PPV-Rec, PPV-Rec + ACLSV, PPV-Rec + PDV, and PPV-Rec + ACLSV + PDV. Severe PPV symptoms appeared in leaves of shoots growing from infected buds used for inoculation. During the following three years, further weakening of PPV symptoms was observed in transgenic plants. In 2007, very mild PPV symptoms were found in only a few leaves, and over 60%, resp. 70% of the C5 trees showed no PPV symptoms. The presence of PPV was confirmed by ELISA, ISEM and RT-PCR. No difference in PPV symptoms was observed between PPV-Rec and combinations PPV-Rec + ACLSV, PPV-Rec + PDV, PPV-Rec + ACLSV + PDV. No symptoms of ACLSV appeared in combinations of ACLSV with PPV-Rec and PPV-Rec + PDV during 2004–2007, but the presence of ACLSV in leaves of transgenic plants clone C5 was proved by ELISA and RT-PCR. Neither synergistic nor antagonistic effects of ACLSV on PPV-Rec were observed. No symptoms of PDV appeared in combinations of viruses with PDV during 2004–2007. PDV was not detected by ELISA, and the presence of PDV was uncertain by RT-PCR in most of inoculated trees in 2006 and 2007. The results of RT-PCR will be further confirmed by sequence analysis and discussed. These results suggest a possible antagonistic interaction between PPV-Rec and PDV in plum clone C5.
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2

Neyt, Mattias, Imgard Vinck, Marc Gewillig i Hans Van Brabandt. "Percutaneous pulmonary and aortic valve insertion in Belgium: Going for conditional reimbursement or waiting for further evidence?" International Journal of Technology Assessment in Health Care 25, nr 03 (lipiec 2009): 281–89. http://dx.doi.org/10.1017/s0266462309990080.

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Objectives:The aim of this study was to assess current evidence supporting the use of percutaneous heart valves (PHV) in degenerative aortic valve and congenital pulmonary outflow tract disease, as compared to conservative medical therapy or traditional surgical valve replacement.Methods:A systematic review of the literature on PHV was performed.Results:No randomized controlled trials (RCT) on PHV have been published so far. Only observational data from series and data presented at cardiology meetings are available. Both percutaneous aortic valve (PAV) and percutaneous pulmonary valve (PPV) seem feasible in the hands of an experienced team. Safety, however, seems to be a problem in PAV, as shown by the high 30-day and 6-month mortality rates.Conclusions:Due to safety concerns, PAV reimbursement is not recommended and patients should only be subjected to PAV insertion within the boundaries of an RCT. In contrast, PPV implantation seems to be a safe and promising technology for which reimbursement under strict conditions may be recommended.
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3

Krakowka, S., J. A. Ellis, B. Meehan, S. Kennedy, F. McNeilly i G. Allan. "Viral Wasting Syndrome of Swine: Experimental Reproduction of Postweaning Multisystemic Wasting Syndrome in Gnotobiotic Swine by Coinfection with Porcine Circovirus 2 and Porcine Parvovirus". Veterinary Pathology 37, nr 3 (maj 2000): 254–63. http://dx.doi.org/10.1354/vp.37-3-254.

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One-day-old gnotobiotic piglets were inoculated intranasally with in vitro passaged porcine circovirus 1 (PCV-1), PCV-2, and porcine parvovirus (PPV) alone or in combination (PCV-1/PCV-2, PCV-1/PPV, and PCV-2/PPV). Piglets were evaluated for 1) the development of porcine postweaning multisystemic wasting syndrome (PMWS), 2) distribution of viral antigens by immunochemistry, and 3) viremia and the presence of viral DNA in nasal and ocular secretions and feces. All single agent-infected piglets and piglets infected with PCV-1/PCV-2 or PCV-1/PPV were clinically asymptomatic. They were transiently viremic and seroconverted to homologous virus(es). At termination of the study on postinfection day (PID) 35, microscopic lesions were restricted to focal inflammatory cell infiltrates in livers and myocardia. One piglet given PCV-1/PPV was PPV viremic for 2 weeks after infection and had lymphangiectasia of the spiral and descending colon associated with granulomatous inflammation. All four PCV-2/PPV-inoculated piglets developed PMWS, characterized by sudden onset of depression and anorexia, icterus, and submucosal edema. One piglet became moribund on PID 27, and the remaining three piglets were euthanatized between PID 27 and PID 30 because of severe disease. Lymph nodes were small and the livers were mottled. Disseminated angiocentric granulomatous inflammation was present in all tissues examined except the brain. Multiple lightly basophilic intracytoplasmic inclusion bodies were identified in macrophages and histiocytes. PCV-2 antigen was widely distributed within macro-phages; PPV antigen was sparse. Hepatocellular necrosis and bile retention were prominent. PCV-2 DNA was identified in ocular, fecal, and nasal secretions. Terminal sera contained antibodies to PPV (4/4) and PCV-2 (3/4). Production of PMWS in gnotobiotic swine appears to require PCV-2 and additional infectious agents such as PPV for full disease expression in gnotobiotic piglets.
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4

Duarte, Geraldo, Petronella Muresan, Shawn Ward, Lauren Laimon, Stephen I. Pelton, Jennifer Canniff, Amanda Golner i in. "Immunogenicity of Conjugated and Polysaccharide Pneumococcal Vaccines Administered During Pregnancy or Postpartum to Women With HIV". Journal of Infectious Diseases 225, nr 6 (6.11.2021): 1021–31. http://dx.doi.org/10.1093/infdis/jiab567.

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Abstract Background Pneumococcal vaccination is recommended in people with HIV, prioritizing PCV. We compared the immunogenicity of PCV-10 and PPV-23 administered antepartum or postpartum. Methods This double-blind study randomized 346 pregnant women with HIV on antiretrovirals to PCV-10, PPV-23, or placebo at 14–34 weeks gestational age. Women who received placebo antepartum were randomized at 24 weeks postpartum to PCV-10 or PPV-23. Antibodies against 7 serotypes common to both vaccines and 1 serotype only in PPV-23 were measured by ELISA/chemiluminescence; B- and T-cell responses to serotype 1 by FLUOROSPOT; and plasma cytokines/chemokines by chemiluminescence. Results Antibody responses were higher after postpartum versus antepartum vaccination. PCV-10 generated lower antibody levels than PPV-23 against 4 and higher against 1 of 7 common serotypes. Additional factors associated with high postvaccination antibody concentrations were high prevaccination antibody concentrations and CD4+ cells; low CD8+ cells and plasma HIV RNA; and several plasma cytokines/chemokines. Serotype 1 B- and T-cell memory did not increase after vaccination. Conclusions Antepartum immunization generated suboptimal antibody responses, suggesting that postpartum booster doses may be beneficial and warrant further studies. Considering that PCV-10 and PPV-23 had similar immunogenicity, but PPV-23 covered more serotypes, use of PPV-23 may be prioritized in women with HIV on antiretroviral therapy. Clinical Trails Registration NCT02717494.
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5

Yakhanthip, Thanisorn, Nawee Kungwan, Jitrayut Jitonnom, Piched Anuragudom, Siriporn Jungsuttiwong i Supa Hannongbua. "Theoretical Investigation on the Electronic and Optical Properties of Poly(fluorenevinylene) Derivatives as Light-Emitting Materials". International Journal of Photoenergy 2011 (2011): 1–9. http://dx.doi.org/10.1155/2011/570103.

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Density functional theory (DFT) and time-dependent DFT (TDDFT) were employed to study ground-state properties, HOMO-LUMO gaps(ΔH-L), excitation energies(Eg), ionization potentials (IPs), and electron affinities (EA) for PFV-alt-PDONV and PFV-alt-PDIH-PPV having different alternating groups. Excited-state properties were investigated using configuration interaction singles (CISs) while fluorescence energies were calculated using TDDFT. The results show that PFV-alt-PDONV exhibits blue-shifted energies for both HOMO-LUMO gaps(ΔH-L)and excitation energies(Eg)compared with PFV-alt-PDIH-PPV. The predicted IP and EA clearly indicate that PFV-alt-PDIH-PPV has both easier hole creation and electron injection than that of PFV-alt-PDONV. The maximal absorption wavelengths of all polymers are strongly assigned toπ→π∗transition. The predicted radiative lifetimes of PFV-alt-PDONV and PFV-alt-PDIH-PPV for B3LYP/6-31G(d) are 0.36 and 0.61 ns, respectively, indicating that PFV-alt-PDIH-PPV should have a better performance for long-time emission than that of PFV-alt-PDONV.
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6

Polák, J. "Viruses of blackthorn and road-bordering trees of plum, myrobalan, sweet and sour cherries in the Czech Republic". Plant Protection Science 43, No. 1 (7.01.2008): 1–4. http://dx.doi.org/10.17221/2351-pps.

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The distribution of <i>Plum pox virus</i> (PPV), <i>Prune dwarf virus</i> (PDV), <i>Prunus necrotic ringspot virus</i> (PNRSV), <i>Apple chlorotic ringspot virus</i> (ACLSV) and <i>Apple mosaic virus</i> (ApMV) in naturally growing shrubs of blackthorn and road-bordering trees of plum and myrobalan, and of PPV, PDV, PNRSV and <i>Cherry leafroll virus</i> (CLRV) in sweet and sour cherry trees were investigated. The most widely distributed viruses were PPV in plums (74% of the investigated trees were infected); PPV, PDV, and PNRSV in myrobalans (26%, 11% and 18%, respectively), PDV in blackthorns (27%), and PDV and PNRSV in cherries (25% and 22%). PPV was not detected in sweet and sour cherries. The incidence of ACLSV and ApMV was negligible in individually growing trees of the genus Prunus in the Czech Republic.
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7

Koyama, S., W. J. Lamm, J. Hildebrandt i R. K. Albert. "Flow characteristics of open vessels in zone 1 rabbit lungs". Journal of Applied Physiology 66, nr 4 (1.04.1989): 1817–23. http://dx.doi.org/10.1152/jappl.1989.66.4.1817.

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To describe the flow characteristics of vessels open in zone 1, we perfused isolated rabbit lungs with Tyrode's solution containing 1% albumin, 4% dextran, and papaverine (0.05 mg/ml). Lungs were expanded by negative pleural pressure (Ppl) of -10, -15, -20, and -25 cmH2O. Pulmonary arterial (Ppa) and venous (Ppv) pressures were varied relative to alveolar pressure (PA = 0) and measured 5–10 mm inside the pleura (i) and outside (o) of the lungs. With Ppa(o) at -2.5 cmH2O, we constructed pressure-flow (P-Q) curves at each Ppl by lowering Ppv(o) until Q reached a maximum, indicating fully developed zone 1 choke flow. Maximum flows were negligible until Ppl fell below -10 cmH2O, then increased rapidly at Ppl of -15 and -20 cmH2O, and at Ppl of -25 cmH2O reached about 15 ml.min-1.kg body wt-1. The Ppv(o) at which flow became nearly constant depended on degree of lung inflation and was 5–8 cmH2O more positive than Ppl. As Ppv(o) was lowered below Ppa(o), Ppv(i) remained equal to Ppv(o) until Ppv(i) became fixed at a pressure 2–3 cmH2O more positive than Ppl. At this point the choke flow was therefore located in veins near the pleural boundary. No evidence of choke flow (only ohmic resistance) was seen in the intrapulmonary segment of the vessels remaining open in zone 1. With Ppv(o) held roughly at Ppl, Q could be stopped by lowering Ppa(o), at which time Ppa(i) was several cmH2O above Ppv(i), showing that intrapulmonary vessel closure had occurred.(ABSTRACT TRUNCATED AT 250 WORDS)
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8

Gordan, Lucio N., Basit Iqbal Chaudhry, Maen A. Hussein, Nora Connor, Andrew Yue, Michael Diaz, T. R. Strickland, Lisa Tran i Nathan Walcker. "Practice transformation at scale through a microsystems quality improvement (QI) approach." Journal of Clinical Oncology 39, nr 15_suppl (20.05.2021): e18667-e18667. http://dx.doi.org/10.1200/jco.2021.39.15_suppl.e18667.

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e18667 Background: How oncology providers should implement practice transformation for value-based care is unclear, particularly at scale. Organizational size enables efficient “top down” approaches, but also presents challenges such as physician engagement. Dis-economies of scale can be acute in oncology due to physician autonomy and coordination costs. We hypothesized that organizational change based in sense-making models that enhance physician engagement and use a decentralized, iterative microsystems approach will enable practice transformation to scale. Methods: Florida Cancer Specialists & Research Institute (FCS) is a physician led 250-oncologist statewide practice, with regional variation in disease state/mix, patient cohort, etc., making a purely top-down approach to organizational change infeasible. FCS prototyped a transformation strategy starting in June 2017 based on sharing interpreted data with physician and executive leadership. Later implementation directly engaged physicians in a microsystems quality QI strategy focused on regional performance. Interventions targeted disease, health service utilization, location, and individual physicians. Performance was evaluated using data from Medicare’s Oncology Care Model (OCM) and assessed using the one-sided risk target (4% below benchmark). We analyzed 70,239 performance period (PP) episodes at FCS across 35,116 patients. Results: In the pre- intervention period (90% of PP1 episodes, completed by June 2017), FCS was 5.8% above target. Performance was 10.9% above target for the remainder of PP1 (10% of PP1 episodes), then improved to 0.3% above target in PP2 and PP3, and below target by 0.9%, 0.8%, and 0.75% in PP4, PP5, and PP6. Early QI efforts focused on performance in lung cancer, which was 2.5% over target in PP1; it improved to 2.1% under target in PP6. Later regional QI sessions targeted cancer, utilization and providers. Pre-intervention, all 18 regions were above target; by PP6, 11 out of 19 regions were below target. Relative to the pre-intervention period, per-episode inpatient costs increased by 12.1% for the remainder of PP1 and increased by 4.3% and 1.3% in PP2 and PP6; inpatient costs decreased in PP3, PP4, and PP5 by 3.8%, 2.4% and 4.8%. Conclusions: Practice transformation in oncology can achieve scale through models of organizational change that foster physician engagement. Data, when clinically contextualized, is a foundational tool in the sense-making process. Scale can develop through an additive microsystems approach in which QI units are de-centralized, accountability is defined, and iteration becomes part of organizational culture. [Table: see text]
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9

Rose, Markus A., Ralf Schubert, Nicola Strnad i Stefan Zielen. "Priming of Immunological Memory by Pneumococcal Conjugate Vaccine in Children Unresponsive to 23-Valent Polysaccharide Pneumococcal Vaccine". Clinical Diagnostic Laboratory Immunology 12, nr 10 (październik 2005): 1216–22. http://dx.doi.org/10.1128/cdli.12.10.1216-1222.2005.

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ABSTRACT Pneumococcal polysaccharide vaccine (PPV) is of limited immunogenicity in infants and immunocompromised patients. Our prospective randomized controlled trial investigated whether priming with pneumococcal conjugate vaccine (PCV) induced specific immunological memory in previously nonresponders to PPV. Of a total of 33 children (2 to 18 years) with polysaccharide-specific immunodeficiency (PSI), group A (n = 16) received two doses of 7-valent PCV in a 4- to 6-week interval, and a booster dose of 23-valent PPV after one year. Group B (n = 17) received two doses of PPV in a 1-year interval exclusively. Specific antibody concentrations for serotypes 4, 5, 6B, 9V, 14, 18C, 19F, and 23F were determined (enzyme-linked immunosorbent assay) before and at 7 and 28 days after administration of the PPV booster and compared to an opsonophagocytosis assay. Of group A, 64 to 100% had antibody concentrations of ≥1 μg/ml on day 28 after the booster versus 25 to 94% of group B. Group A had significantly higher antibody concentrations for all PCV-containing serotypes already on day 7, indicating early memory response. Antibody concentrations were in accordance with functional opsonic activity, although opsonic titers varied among individuals. Pneumococcal vaccination was well tolerated. The incidence of airway infections was reduced after priming with PCV (10/year for group A versus 15/year for group B). Following a PPV booster, even patients primarily not responding to PPV showed a rapid and more pronounced memory response after priming with PCV.
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10

Okić, Arnela, Thierry Wetzel, Shaheen Nourinejhad Zarghani, Sébastien Massart, Jasmin Grahić, Fuad Gaši, Almira Konjić i Darko Vončina. "Occurrence and Distribution Patterns of Plum Tree Viruses and Genetic Diversity of Sharka Isolates in Bosnia and Herzegovina". Horticulturae 8, nr 9 (28.08.2022): 783. http://dx.doi.org/10.3390/horticulturae8090783.

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In order to fill in a decade-long information gap regarding the biological, serological and molecular data for plum tree viruses in Bosnia and Herzegovina, a three-phase study combining symptom evaluation, and serological and molecular assays with high-throughput sequencing (HTS) technology was conducted. The most frequently observed symptoms were discolorations in the form of ring patterns, bands and irregular shapes, as well as vein banding. Sharka-associated symptoms in the form of ring patterns and semicircles were prevalent. A total of 468 plum tree samples were tested by ELISA for the presence of PPV, ApMV, PDV, PNRSV, PBNSPaV, ACLSV and MLRSV. An overall infection incidence of 51.9% was detected, with PPV being the most prevalent (48.7%), followed by PDV (2.99%), PNRSV (0.21%) and mixed infections of PPV+PDV (1.71%). RT-PCR-assisted strain typing in 45 samples revealed PPV-D as the most common strain (22.22%), followed by PPV-REC (6.66%). Mixed infections of PPV-D+PPV-REC were detected (6.66%). HTS enabled the recovery of a 9743 nts long sequence of PPV-D (PPV_O7/80, MW412433), which shared the highest nucleotide and amino acid identities with isolates S13 (LC375131) from Serbia, SVN1 (LC375132) from Slovenia and N9 (LC375129) from Bulgaria. The phylogenetic analysis of the whole genome placed the isolate of the D strain in a distinctive group with the Slovenian isolate SVN1 (LC375132). In addition, the (Cter)NIb/(Nter)CP fragment of a PPV-REC isolate (MW412434) obtained in this survey formed a separate group with previously known isolates from Bosnia and Herzegovina (BOS64Pl and BOS257Pl).
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11

Kang, H. S., K. H. Kim, M. S. Kim, K. T. Park, K. M. Kim, T. H. Lee, J. Joo, K. Kim, D. W. Lee i J. I. Jin. "Electrical and optical characteristics of various PPV derivatives (MEH-PPV, CzEH-PPV, OxdEH-PPV)". Current Applied Physics 1, nr 6 (grudzień 2001): 443–46. http://dx.doi.org/10.1016/s1567-1739(01)00055-4.

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12

Bastians, H., i H. Ponstingl. "The novel human protein serine/threonine phosphatase 6 is a functional homologue of budding yeast Sit4p and fission yeast ppe1, which are involved in cell cycle regulation". Journal of Cell Science 109, nr 12 (1.12.1996): 2865–74. http://dx.doi.org/10.1242/jcs.109.12.2865.

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We identified a novel human protein serine/threonine phosphatase cDNA, designated protein phosphatase 6 (PP6) by using a homology-based polymerase chain reaction. The predicted amino acid sequence indicates a 35 kDa protein showing high homology to other protein phosphatases including human PP2A (57%), human PP4 (59%), rat PPV (98%), Drosophila PPV (74%), Schizosaccharomyces pombe ppe1 (68%) and Saccharomyces cerevisiae Sit4p (61%). In human cells, three forms of PP6 mRNA were found with highest levels of expression in testis, heart and skeletal muscle. The PP6 protein was detected in lysates of human heart muscle and in bull testis. Complementation studies using a temperature sensitive mutant strain of S. cerevisiae SIT4, which is required for the G1 to S transition of the cell cycle, showed that PP6 can rescue the mutant growth arrest. In addition, a loss of function mutant of S. pombe ppe1, described as a gene interacting with the pim1/spi1 mitotic checkpoint and involved in cell shape control, can be complemented by expression of human PP6. These data indicate that human PP6 is a functional homologue of budding yeast Sit4p and fission yeast ppe1, implying a function of PP6 in cell cycle regulation.
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13

Chen, Jinbin, Kangqiang Peng, Dandan Hu, Jingxian Shen, Zhongguo Zhou, Li Xu, Jiancong Chen i in. "Tumor Location Influences Oncologic Outcomes of Hepatocellular Carcinoma Patients Undergoing Radiofrequency Ablation". Cancers 10, nr 10 (10.10.2018): 378. http://dx.doi.org/10.3390/cancers10100378.

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Radiofrequency ablation (RFA) is recommended as a first-line therapy for small hepatocellular carcinoma (HCC). Tumor location is a potential factor influencing the procedure of RFA. To compare oncologic outcomes of RFA for different tumor locations, this retrospective study enrolled 194 patients with small HCC who had undertaken RFA. The HCC nodules were classified as peri-hepatic-vein (pHV) or non-pHV, peri-portal-vein (pPV) or non-pPV, and subcapsular or non-subcapsular HCC. The regional recurrence-free survival (rRFS), overall survival (OS), recurrence-free survival (recurrence in any location, RFS) and distant recurrence-free survival (dRFS) were compared. Operation failures were recorded in five pPV HCC patients, which was more frequent than in non-pPV HCC patients (p = 0.041). The 1-, 3-, and 5-year rRFS was 68.7%, 53.7%, and 53.7% for pHV patients and 85.1%, 76.1%, and 71.9% for non-pHV patients, respectively (p = 0.012). After propensity score matching, the 1-, 3-, and 5-year rRFS was still worse than that of non-pHV patients (p = 0.013). The OS, RFS, and dRFS were not significantly different between groups. Conclusions: A pHV location was a risk factor for the regional recurrence after RFA in small HCC patients. The tumor location may not influence OS, RFS, and dRFS. Additionally, a pPV location was a potential high-risk factor for incomplete ablation.
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Lamm, W. J., i R. K. Albert. "Continuity of arterial and venous extra-alveolar interstitium in excised rabbit lungs". Journal of Applied Physiology 63, nr 2 (1.08.1987): 634–38. http://dx.doi.org/10.1152/jappl.1987.63.2.634.

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We studied the interdependence of arterial and venous extra-alveolar vessel (EAV) leakage on the rate of pulmonary vascular fluid filtration (measured as the change in lung weight over time). Edema was produced in continually weighed, excised rabbit lungs kept in zone 1 (alveolar pressure = 25 cmH2O) by increasing pulmonary arterial (Ppa) and/or venous (Ppv) pressure from 5 to 20 cmH2O (relative to the lung base) and continuing this hydrostatic stress for 3–5 h. Raising Ppa and Ppv simultaneously produced a lower filtration rate than the sum of the filtration rates obtained when Ppa and Ppv were raised separately, while the lung gained from 20 to 95% of its initial weight. When vascular pressure was elevated in either EAV segment, fluid filtration always decreased rapidly as the lung gained up to 30–45% of its initial weight. Filtration then decreased more slowly. The lungs became isogravimetric at 60 and 85% weight gain when the Ppa or Ppv was elevated, respectively; when Ppa and Ppv were raised simultaneously substantial fluid filtration continued even after 140% weight gain. We conclude that the arterial and venous EAV's share a common interstitium in the zone 1 condition, this interstitium cannot be represented as a single compartment with a fixed resistance and compliance, and arterial and venous EAV leakage influences leakage from the other segment.
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Muravyev, Alexander A., Aida N. Chagaryan, Nataly V. Ivanchik, Anastasia A. Kurkova, I. A. Tsvetkova, Roman S. Kozlov, O. A. Morozova i in. "The prevalence of circulating S. pneumoniae serotypes in people older than 18 years: healthy carriers, patients with acute otitis media, community-acquired pneumonia, and invasive pneumococcal infections (epidemiological study «SPECTRUM»)". Clinical Microbiology and Antimicrobial Chemotherapy 21, nr 4 (2019): 275–81. http://dx.doi.org/10.36488/cmac.2019.4.275-281.

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Objective. To estimate the prevalence of S. pneumoniae serotypes in adults aged of 18 years and older, including healthy carriers and patients with acute otitis media (AOM), community-acquired pneumonia (CAP) and invasive pneumococcal infection (IPI). Materials and Methods. A total of 230 isolates of S. pneumoniae from 10 centers were included in the study from 01.06.2019 to 01.10.2019. Re-identification and typing using real-time PCR with 22 primer pairs were performed in the central laboratory (Institute of Antimicrobial Chemotherapy, Russia). Results. In healthy nasopharyngeal carriers (n = 31), the following serotypes of S. pneumoniae were detected: 19F (29.0%), 6А/В (22.6%), 3 (16.1%), 11A/D and 23F (9.7% each), 23A (6.4%), 14 and 15A/F (3.2% each); PCV-13 and PPV-23 covered 80.6% and 90.3% of those serotypes, respectively. In patients with AOM (n = 18), serotypes were: 3 (16.7%), 11A/D and 6A/B (11.1%, each), 16, 19А, 19F, 23F, 7A/F (5.55%, each); PCV-13 and PPV-23 covered 50% and 61.1% of those serotypes, respectively. Among 166 isolates from patients with CAP, the following serotypes were detected: 3 (12.0%), 19F and 6A/B (10.2%, each), 14 and 11A/D (5.4% each), 15A/F and 23A (4.8% each), 9N/L (4.2%), 18 and 22A/F (2.4% each), 19A and 23F (1.8% each), 16, 9V/A (1.2% each), 4 and 33A/33F/37 (0.6% each), non-vaccine serotypes (30.3%); PCV-13 and PPV-23 covered 45.6% and 57.0% of those serotypes, respectively. Isolates from patients with IPI (n=15) belonged to the following serotypes: 3 (26.7%), 12F, 23F and 9N/L (13.3% each), serotypes 4 and 15A/F (6.7%), non-vaccine serotype – 1 isolate (6.7%); PCV-13 and PPV-23 covered 46.7% and 73.3% of those serotypes, respectively. Conclusions. The majority of S. pneumoniae serotypes in adult population in Russia are included in PPV-23, but not in PCV-13 (primarily due to serotype 11A/D). There was a high PCV-13 and PPV-23 coverage of serotypes from healthy nasopharyngeal carriers. PPV-23 covered more than 60% of clinical isolates, whereas PCV-13 covered less than 60% (AOM – 50.0%, CAP – 45.6%, IPI – 46.7%), thus indicating a potentially lower efficacy of PCV-13 in adult population.
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Beck, K. C., i S. J. Lai-Fook. "Pulmonary blood flow vs. gas volume at various perfusion pressures in rabbit lung". Journal of Applied Physiology 58, nr 6 (1.06.1985): 2004–10. http://dx.doi.org/10.1152/jappl.1985.58.6.2004.

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To obtain a detailed description of the dependence of pulmonary blood flow on changes in lung volume, we perfused isolated rabbit lungs with homologous blood at 37 degrees C while controlling vascular pressures during lung deflation. We set pulmonary arterial pressure (Ppa) and pulmonary venous pressure (Ppv) to constant values relative to alveolar pressure (Palv) to keep the effective driving pressure for flow constant during lung deflation from total lung capacity (TLC) to 25% TLC. The shapes of the flow vs. lung volume curves were dependent on the levels of Ppa-Palv and Ppv-Palv at which they were obtained. When Ppv greater than Palv throughout the lung (zone 3 conditions), flow increased as the lungs were deflated from TLC, independent of the level of Ppa-Palv. When Ppv less than Palv (zone 2 conditions) and Ppa-Palv was moderately high, flow increased as the lungs were deflated from 100 to approximately 50% TLC, then decreased at lower lung volumes. When Ppa - Palv was less than 10 cmH2O in zone 2 conditions, flow decreased monotonically during deflation from TLC. We concluded that the dependence of blood flow on lung volume is complex, which may be a reflection of the nonlinear pressure-diameter properties of pulmonary vessels.
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ZAGRAI, Luminița A., Ioan ZAGRAI, Georgeta M. GUZU, Smaranda D. ROȘU-MAREȘ i Claudiu MOLDOVAN. "Assessment of the virus infections occurrence in new established plum and sweet cherry orchards in Transylvania, Romania". Notulae Botanicae Horti Agrobotanici Cluj-Napoca 50, nr 2 (30.06.2022): 12734. http://dx.doi.org/10.15835/nbha50212734.

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Sixteen plum and eleven sweet cherry newly established commercial orchards in Transylvania, Romania, were the subject of the survey during the vegetative periods of 2020-2021 in order to assess the occurrence of different virus infections. Two blocks of 200 trees from each orchard were monitored by visual observation for virus-like symptoms, especially for Plum pox virus (PPV) in plum orchards. Twenty trees of each plum orchard were then sampled and tested for serological detection of PPV, Prune dwarf virus (PDV), Prunus necrotic ringspot virus (PNRSV), Apple chlorotic leaf spot virus (ACLSV), Apple mosaic virus (ApMV) and Myrobalan latent ringspot virus (MLRSV). Similarly, ten trees of each sweet cherry orchard were sampled and tested for the presence of PDV, PNRSV, ApMV, ACLSV, PPV, Arabis mosaic virus (ArMV), Cherry leaf roll virus (CLRV), Raspberry ringspot virus (RpRSV), Strawberry latent ringspot virus (SLRSV) and Tomato black ring virus (TBRV) by serologic assays. Additionally, a few sweet cherry trees suspected to be infected by Little cherry virus-1 (LChV-1) were tested by molecular assay. Unexpectedly, no plum orchards were found to be free of PPV. The average level of PPV infection was 32%. PNRSV occurred in 8.1% and PDV in 1.2% of sampled plum trees. Four out of ten viruses were detected in sweet cherry sampled: PDV (3.6%), ACLSV (0.9%), RpRSV (0.9%) and TBRV (0.9%). No infection with ACLSV, ApMV and MLRSV in plum and PPV, PNRSV, ApMV, ArMV, CLRV, SLRSV and LChV-1 in sweet cherry was detected. Mixed infections occurred at 4.8% in plum, and at 0.9% in sweet cherry trees sampled. The average occurrence of viruses in plum and sweet cherry orchards surveyed in Transylvania was determined at 41.3%, and 6.3% respectively. Overall results revealed a critical situation especially in regards to PPV infections making the success of fruit production in the most surveyed plum orchards quite problematic. Virus infections level in the younger sweet cherry orchards were significantly lower.
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ZAGRAI, Ioan, Luminița ZAGRAI i Georgeta GUZU. "Assessment of Phytoviral Status of some New Plum Orchards Established in Iaşi County, Romania". Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca. Horticulture 77, nr 2 (18.11.2020): 155. http://dx.doi.org/10.15835/buasvmcn-hort:2020.0037.

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Four young plum orchards from Iaşi county, two of these established by using propagated material in Romania, and the other two with material from Austria, Czech Republic and Netherlands, were surveyed by visual observation of viral symptoms. Then, trees were sampled and tested for the presence of six viruses (PPV, PDV, PNRSV, ACLSV, ApMV and MLRSV) by serologic assays. A high rate of PPV infection (78% and 79%, respectively) was determinated in the two orchards established with planting material propagated in Romania, and a rate of 5% PDV infection was found in one of them. A rate of 2.5% PPV infection was determined in the orchard established with propagated material in Austria and Czech Republic, and no PPV infected tree was found in the orchard set up with material propagated in Netherlands. The infections with PNRSV, ACLSV, ApMV and MLRSV were not detected in the four orchards surveyed.
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Martin, S. J., Helen Mellor, Donal D. C. Bradley i P. L. Burn. "Electroabsorption studies of PPV and MEH-PPV". Optical Materials 9, nr 1-4 (styczeń 1998): 88–93. http://dx.doi.org/10.1016/s0925-3467(97)00153-5.

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Damsteegt, V. D., H. E. Waterworth, G. I. Mink, W. E. Howell i L. Levy. "Prunus tomentosa as a Diagnostic Host for Detection of Plum Pox Virus and Other Prunus Viruses". Plant Disease 81, nr 4 (kwiecień 1997): 329–32. http://dx.doi.org/10.1094/pdis.1997.81.4.329.

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The efficacy of seedlings of Prunus persica cv. GF 305, P. persica cv. Siberian C, and P. tomentosa (Nanking Cherry) as diagnostic indicators of plum pox infection, and of P. tomentosa for other Prunus viruses was evaluated by graft-inoculation with eight different strains or isolates of plum pox virus (PPV) representative of the Marcus (M) and Dideron (D) serogroups; and one isolate each of Prunus necrotic ringspot virus (PNRSV), prune dwarf virus (PDV), and sour cherry green ring mottle virus (GRMV). The initial PPV symptoms that developed in P. tomentosa within 30 days after inoculation were chlorotic banding along the midrib spreading to lateral veins from the leaf base upward, giving the appearance of a chlorotic oak-leaf pattern. Symptoms caused by PPV-M could be distinguished from those caused by PPV-D. Virus titers in infected P. tomentosa and GF 305 were higher than those in Siberian C when measured by triple antibody sandwich enzyme-linked immunosorbent assay. Infections by PNRSV, PDV, and GRMV were evident with the first flush of vegetative growth.
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&NA;. "PPV revaccination". Inpharma Weekly &NA;, nr 1172 (styczeń 1999): 19. http://dx.doi.org/10.2165/00128413-199911720-00044.

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Polák, J., J. Kumar, B. Krška i M. Ravelonandro. "Biotech/GM crops in horticulture: plum cv. HoneySweet resistant to Plum pox virus". Plant Protection Science 48, Special Issue (12.12.2012): S43—S48. http://dx.doi.org/10.17221/37/2012-pps.

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Commercialisation of Biotech/GM (Biotech) crops started in 1995. Not only field crops, but also horticultural transgenic crops are under development and are beginning to be commercialised. Genetic engineering has the potential to revolutionise fruit tree breeding. The development of transgenic fruit cultivars is in progress. Over the past 20 years an international public sector research team has collaborated in the development of HoneySweet plum which is highly resistant to Plum pox virus (PPV) the most devastating disease of plums and other stone fruits. HoneySweet was deregulated in the USA in 2010. HoneySweet (aka C5) has been evaluated for eleven years (2002&ndash;2012) in a regulated field trial in the CzechRepublic for the resistance to PPV, Prune dwarf virus (PDV), and Apple chlorotic leaf spot virus (ACLSV), all of them being serious diseases of plum. Even under the high and permanent infection pressure produced through grafting, PPV&nbsp;has only been detected in HoneySweet trees in several leaves and fruits situated close to the point of inoculum grafting. The lack of infection spread in HoneySweet demonstrates its high level of PPV resistance. Co-infections of PPV with PDV and/or ACLSV had practically no influence on the quantity and quality of HoneySweet fruit which are large, sweet, and of a high eating quality. In many respects, they are superior to the fruits of the well-known cultivar Stanley. Many fruit growers and fruit tree nurseries in the CzechRepublic are supportive of the deregulation of HoneySweet plum to help improve the plum production and control the spread of PPV.
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Olivati, Clarissa De Almeida, Rodrigo Fernando Bianchi, Flavia Maria Marconi, Debora Terezia Balogh i Roberto Mendonca Faria. "Photoconduction Effect on PPV and MH-PPV Structures". Molecular Crystals and Liquid Crystals 374 (2002): 451–56. http://dx.doi.org/10.1080/713738268.

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OLIVATI, CLARISSA DE ALMEIDA, RODRIGO FERNANDO BIANCHI, FLAVIA MARIA MARCONI, DEBORA TEREZIA BALOGH i ROBERTO MENDONçA FARIA. "Photoconduction Effect on PPV and MH-PPV Structures". Molecular Crystals and Liquid Crystals 374, nr 1 (1.01.2002): 451–56. http://dx.doi.org/10.1080/10587250210484.

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Kozlov, Roman S., Alexander A. Muravyev, Aida N. Chagaryan, Nataly V. Ivanchik, Anastasia A. Kurkova, Alexey Yu Kuzmenkov, Ivan V. Trushin i Marina V. Sukhorukova. "The prevalence and antimicrobial susceptibility of circulating S. pneumoniae serotypes in adult population in Russia (epidemiological study «SPECTRUM»)". Clinical Microbiology and Antimicrobial Chemotherapy 23, nr 2 (2021): 127–37. http://dx.doi.org/10.36488/cmac.2021.2.127-137.

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Objective. To estimate prevalence and antimicrobial susceptibility of circulated S. pneumoniae serotypes in adult population in different regions of the Russian Federation. Materials and Methods. A total of 500 isolates of S. pneumoniae obtained from patients with invasive pneumococcal disease (IPD), community-acquired pneumonia (CAP), sinusitis/acute otitis media (AOM) and healthy carriers from 29 centers were included in the study from 01 June 2019 to 15 January 2020. Re-identification, typing using real-time PCR with 27 primer pairs and antimicrobial susceptibility testing were performed in the central laboratory by standardized methods. Results. Among 50 isolates from patients with IPD, the following serotypes were detected: 3 (20.0%), 23F (10.0%), 6ABCD (8.0%), 19F (6.0%), 12ABF, 4, 7AF, 8, 9NL, 9VA, 15B (4.0% each), 1, 10A, 11AD, 14, 15AF, 18ABCF, 22AF, 33F/33A/37 (2.0% each). PCV-13 and PPV-23 covered 62.0% and 86.0% of those serotypes, respectively. Among 357 isolates from patients with CAP, the following serotypes were detected: 19F (12.3%), 6ABCD (11.5%), 3 (10.1%), 23F (5.9%), 14 (5.3%), 22AF (5.0%), 11AD (4.8%), 9NL (3.4%), 15AF (2.8%), 9VA (2.2%), 18ABCF (2.0%), 19A (1.7%), 12ABF (1.4%), 17F (0.8%), 16, 33F/33A/37, 7AF and 8 (0.6% each), 10A and 4 (0,3% each); serotypes were not specified due to the PCR typing protocol limitations for 106 (29.8%) isolates. PCV-13 and PPV-23 covered 51.9% and 68.7% of those serotypes, respectively. In patients with sinusitis/AOM (n = 48), serotypes were: 19F (18.8%), 6ABCD (16.7%), 23F (12.5%), 3 (12.5%), 18ABCF (6.3%), 11AD (4.2%), 14 (4.2%), 22AF (4.2%), 15AF, 4, 9VA (2.1% each). PCV-13 and PPV-23 covered 75.0% and 83.3% of those serotypes, respectively. In healthy nasopharyngeal carriers (n = 45), the following serotypes were detected: 19F (24.4%), 3 (17.8%), 6ABCD (17.8%), 11AD (13.3%), 23F (8.9%), 22AF (6.7%), 14 and 15AF (2.2% each). PCV-13 and PPV-23 covered 71.1% and 91.1% of those serotypes, respectively. Serotypes 14, 19F, and 19A were frequently resistant to three antibiotics – penicillin, erythromycin, and tetracycline. Resistance to respiratory quinolones was very low (0.7%) and detected among serotypes 14 and 9NL only. The majority of non-vaccine serotypes did not have substantial antimicrobial resistance problems. Conclusions. Despite the relatively low number of isolates in all but CAP group and limitations of PCR typing protocol, results of our study support the use of pneumococcal vaccines (PPV-23 and PCV-13) in Russian adult population, with some advantages of PPV-23 over PCV-13.
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Aikawa, Yuri, Takayuki Baba, Tomohiro Nizawa, Hirotaka Yokouchi i Shuichi Yamamoto. "Worsening of Retinal Detachment after Cataract Surgery in the Eye with Persistent Fetal Vasculature". Case Reports in Medicine 2021 (22.02.2021): 1–4. http://dx.doi.org/10.1155/2021/6641161.

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Purpose. To report a case of persistent fetal vasculature (PFV) with a retinal detachment that worsened after cataract surgery. Pars plana vitrectomy (PPV) was performed which reduced the vitreous traction and reattached the retina. Observations. A 20-year-old Myanmarese woman presented with a mature cataract, and her vision was light perception. She underwent uneventful cataract surgery with implantation of an intraocular lens. Her visual acuity improved to 20/200 immediately after the surgery. However, fibrotic tissue was observed between the optic nerve head and the posterior capsule. She was diagnosed with PFV, and she was followed without any intervention. One and a half years after the cataract surgery, she had an advanced retinal detachment which extended over the inferior two quadrants. Her vision deteriorated to 20/400. She underwent PPV, and the PFV tissue was removed which resulted in the reattachment of the retina. The visual acuity improved to 20/60. Conclusions. Surgeons should be aware that it is possible to worsen a retinal detachment after cataract surgery in the eyes with PFV. A simple technique to release the anterior-posterior traction by the PPV was sufficient to achieve the reattachment of the retina.
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Koyama, S., i J. Hildebrandt. "Air interface and elastic recoil affect vascular resistance in three zones of rabbit lungs". Journal of Applied Physiology 70, nr 6 (1.06.1991): 2422–31. http://dx.doi.org/10.1152/jappl.1991.70.6.2422.

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We examined the effect of the air interface on pulmonary vascular resistance (PVR) in zones 1, 2, and 3 by comparing pressure-flow data of air- and liquid-filled isolated rabbit lungs. Lungs were perfused with Tyrode's solution osmotically balanced with 1% albumin and 4% dextran and containing the vasodilator papaverine (0.05 mg/ml). Lung volume was varied by negative pleural pressure form 0 to -25 cmH2O. Pulmonary artery (Ppa) and venous (Ppv) pressures were fixed at various levels relative to the lung base. Alveolar pressure (PA) was always zero, and perfusate flow was measured continuously. In zone 1 Ppa was -2.5 cmH2O and Ppv was -15 cmH2O. In zone 2 Ppa was 10 cmH2O and Ppv was -5 cmH2O. In zone 3 Ppa was 15 cmH2O and Ppv was 8 cmH2O. We found that in zone 1 the interface was essential for perfusion, but in zones 2 and 3 it had much lesser effects. In general, PVR depended almost uniquely (i.e., with small hysteresis) on transpulmonary pressure, whereas a large hysteresis existed between PVR and lung volume. PVR was high in collapsed and especially in atelectatic lungs, fell sharply with moderate inflation, and within the ranges of vascular pressure studied did not rise again toward total lung capacity. These results suggest that in zone 1 the interface maintains the patency of some alveolar vessels, probably in corners. The majority of alveolar septal vessels appears to be exposed directly to PA in zones 2 and 3, because at equal transpulmonary pressure the PVR is similar in the presence or absence of an interface.
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Bigham, Mark, David M. Patrick, Elizabeth Bryce, Sylvie Champagne, Carol Shaw, Wrency Wu, Helen Ng, Diane Roscoe, Jacques Roy i Judy Isaac-Renton. "Epidemiology, Antibiotic Susceptibility and Serotype Distribution ofStreptococcus pneumoniaeAssociated with Invasive Pneumococcal Disease in British Columbia - A Call to Strengthen Public Health Pneumococcal Immunization Programs". Canadian Journal of Infectious Diseases 14, nr 5 (2003): 261–66. http://dx.doi.org/10.1155/2003/924325.

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BACKGROUND: This study examined the epidemiology, antibiotic susceptibility and serotype distribution ofStreptococcuspneumoniaeassociated with invasive pneumococcal disease (IPD) in British Columbia.METHODS: Six hospitals and one private laboratory network participated in a prospective, sentinel laboratory based surveillance study of IPD, between October 1999 and October 2000. At each site,S pneumoniaeisolates were collected and epidemiological data were gathered using a structured questionnaire, for all cases of IPD meeting the study case definition. Isolates were serotyped and tested for antimicrobial susceptibility. Bivariate associations were analyzed and multivariate logistic regression was used to identify independent risk factors associated with hospitalization or death.RESULTS: One hundred three reports and isolates were collected. Seventy-nine per cent of cases were community-acquired, 64% required hospitalization and 5% died. Cases with one or more assessed risk factor for IPD and of female sex were independent variables associated with hospitalization or death. One-third of isolates had reduced penicillin susceptibility and 96% of these represented serotypes contained in the 23-valent pneumococcal polysaccharide vaccine (PPV-23). Overall, 89% of serotypes identified are included in the PPV-23 vaccine and 88% of isolates from children under five years of age are found in the 7-valent pneumococcal conjugate vaccine (PCV-7). Forty-one per cent of cases qualified for publicly funded pneumococcal vaccine and 34% of eligible persons were vaccinated.CONCLUSIONS: Overall, pneumococcal serotypes associated with IPD in this study closely matched serotypes included in PPV-23 products currently licensed in Canada. Most serotypes associated with IPD in children under five years of age are included in a recently licenced PCV-7. One third of isolates demonstrated reduced penicillin susceptibility, most involving serotypes included in PPV-23. Effective delivery of current public health immunization programs using PPV-23 and extending protection to infants and young children using the PCV-7 will prevent many cases of IPD.
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Mulazzi, E., R. Perego, C. Botta, A. Bolognesi, J. Wéry, H. Aarab, E. Faulques i S. Lefrant. "Radiative recombination studies on PPV and PPV SWNT composites". Synthetic Metals 135-136 (kwiecień 2003): 279–80. http://dx.doi.org/10.1016/s0379-6779(02)00907-4.

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Barbosa, Hélder M. C., i Marta M. D. Ramos. "Mesoscopic modelling of 2-CN-PPV/PPV polymer LED". Journal of Materials Science: Materials in Electronics 18, S1 (28.03.2007): 257–61. http://dx.doi.org/10.1007/s10854-007-9191-y.

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31

Bohlen, H. G., R. Maass-Moreno i C. F. Rothe. "Hepatic venular pressures of rats, dogs, and rabbits". American Journal of Physiology-Gastrointestinal and Liver Physiology 261, nr 3 (1.09.1991): G539—G547. http://dx.doi.org/10.1152/ajpgi.1991.261.3.g539.

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We tested the hypotheses that the hepatic venule pressures (Phv), just downstream from the hepatic sinusoids, are closely similar (less than 2 mmHg) either to the portal venous pressure (Ppv), indicating a high hepatic venous resistance, or to the inferior vena cava (Pivc) pressure, indicating a high portal-sinusoidal venous resistance, as reported by previous investigators. A micropipette servo-null pressure measurement technique was used with rats, dogs, and rabbits. Phv, referred to the anatomic level of the vena cava, averaged 5.1 +/- 1.0, 6.4 +/- 1.1, and 5.4 +/- 1.0 (SD) mmHg in the rats, puppies, and rabbits, respectively. Ppv averaged 8.0 +/- 1.4, 10.8 +/- 2.2, and 7.4 +/- 1.5 mmHg, respectively. Norepinephrine infusion into the portal vein (1-5 micrograms.min-1.kg-1) caused Ppv to increase and the portal venous flow to decrease but did not significantly affect Phv. The hepatic venous circuit contributed 44 +/- 17% (rats) and 31 +/- 26% (dogs) of the total liver venous vascular resistance under control conditions. We conclude that the portal and sinusoidal vasculatures are the dominant, but not exclusive, resistance sites of the liver venous vasculature both at rest and during norepinephrine-induced vasoconstriction.
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Gabler, Th, R. Waldhäusl, A. Bräuer, U. Bartuch, R. Stockmann i H. H. Hörhold. "Nonresonant n2 and two-photon-absorption dispersion measurements of DPOP-PPV and DP-PPV/DP-PFV polymer strip waveguides". Optics Communications 137, nr 1-3 (kwiecień 1997): 31–36. http://dx.doi.org/10.1016/s0030-4018(96)00757-2.

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Polák, J., i P. Komínek. "Distribution of Plum pox virus strains in natural sources in the Czech Republic". Plant Protection Science 45, No. 4 (27.12.2009): 144–47. http://dx.doi.org/10.17221/31/2009-pps.

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The distribution of <i>Plum pox virus</i> (PPV) strains, PPV-D, PPV-M and PPV-Rec, was investigated in the Czech Republic in 2005–2008. Fifty-two to ninety-four samples of flowers or leaves of plum, myrobalan and blackthorn trees from different regions were tested in individual years. The presence of PPV was detected by DAS-ELISA with serotype-specific polyclonal antibodies. PPV-M was proved by DASI-ELISA with serotype-specific monoclonal antibodies; PPV-D, PPV-M and PPV-Rec were detected by RT-PCR in leaf samples from PPV infected trees. The presence of PPV-D ranged from 94.6% to 100%, the presence of PPV-M from 0.0% to 2.3% and the presence of PPV-Rec from 0.0% to 3.1% during 2005–2008. More than 95% of analysed samples of PPV were infected with PPV-D and less than 2.5% of analysed samples of PPV were infected with PPV-M or PPV-Rec. The presence of PPV-C was not proved in sweet cherry and sour cherry trees. The presence of PPV-EA was not proved in apricot trees.
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PROTASOV, A. D. "COMPARATIVE EVALUATION OF THE EFFECTIVENESS OF PNEUMOCOCCAL VACCINATION WITH 13-VALENT CONJUGATE AND 23-VALENT POLYSACCHARIDE VACCINE IN PATIENTS WITH COPD". Russian Journal of Allergy 11, nr 4 (15.12.2014): 12–17. http://dx.doi.org/10.36691/rja583.

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Comparative data on the clinical efficacy of vaccination in patients with chronic obstructive pulmonary disease (COPD) against pneumococcal infection using 13-valent conjugate vaccine (PCV-13) or 23-valent polysaccharide vaccine (PPV-23) is cited. Background. To evaluate a clinical efficacy (the number of exacerbations, courses of antimicrobial chemother- apy, hospitalizations) of PCV-13 and PPV-23 in patients with chronic obstructive pulmonary disease (COPD). Methods. The study involved 55 patients with COPD. Patients who met inclusion/exclusion criteria were divided in 2 groups: I group of patients received one dose of the vaccine «Prevenar-13» intramuscularly in the left shoul- der (n=32), II group received one dose of the vaccine «Pneumo-23» intramuscularly in the left shoulder (n=23). We estimated the number of exacerbations per year prior to vaccination and during the year after vaccination, the number of courses of antibiotic therapy for a year prior to vaccination and for a year after immunization, the number of hospitalizations for COPD exacerbation during the year prior to vaccination and during the year after immunization. Results. Vaccination of patients with COPD with PCV-13 or PPV-23 was characterized by the same high clinical efficacy, without the expressed benefits in favor of any drug. Conclusion. Vaccination of patients with COPD against pneumococcal infection with PCV-13 or PPV-23 is char- acterized by a high clinical efficacy. High cost of the 13-valent conjugate pneumococcal vaccine vs a polysac- charide vaccine additionally requires a proof of it’s benefit use in patients with COPD. Until such evidences and their wide representation in the scientific literature should be done vaccination of COPD patients with 13-valent conjugate vaccine is still discussable.
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Chou, Hsuan-Liang, King-Fu Lin i Ding-Chang Wang. "Miscibility and Luminescence Properties of MEH-PPV/DPO-PPV Polyblends". Journal of Polymer Research 13, nr 1 (18.10.2005): 79–84. http://dx.doi.org/10.1007/s10965-005-9017-7.

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Magalhães, Carlos E. T., Marcio M. da Silva, Ranylson M. L. Savedra i Melissa F. Siqueira. "Anisotropic electron mobility in fluorene-PPV and fluorene-MEH-PPV". Molecular Physics 115, nr 3 (21.12.2016): 357–63. http://dx.doi.org/10.1080/00268976.2016.1265679.

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Dietrich, Christof, Qusai Al Abdallah, Lara Lintl, Agnes Pietruszka i Edgar Maiss. "A chimeric plum pox virus shows reduced spread and cannot compete with its parental wild-type viruses in a mixed infection". Journal of General Virology 88, nr 10 (1.10.2007): 2846–51. http://dx.doi.org/10.1099/vir.0.82989-0.

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The effect of a recombination event in the genomic 3′ end on the biological properties and competitiveness of plum pox virus (PPV) was investigated. Therefore, a fragment spanning the coat protein (CP) coding region and a part of the 3′ non-translated region of a non-aphid-transmissible strain of PPV (PPV-NAT) was replaced by the corresponding region of a PPV sour cherry isolate (PPV-SoC). The resulting chimera (PPV-NAT/SoC) caused severe symptoms in Nicotiana benthamiana, resembling those of PPV-NAT. In mixed infections with either of the parental viruses, the chimera PPV-NAT/SoC was less competitive. Labelling experiments with DsRed showed that PPV-NAT/SoC (PPV-NAT/SoC-red) moved more slowly from cell to cell than PPV-NAT (PPV-NAT-red). In mixed infections of PPV-NAT/SoC-red with a green fluorescent protein-expressing PPV-NAT (PPV-NAT-AgfpS), spatial separation of the viruses was observed. These data suggest that, in PPV infections, symptom severity and competitiveness are independent aspects and that spatial separation may contribute to the displacement of a recombinant virus.
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Jevremovic, Darko, i Svetlana Paunovic. "Plum pox virus strains: Diversity and geographical distribution in Serbia". Pesticidi i fitomedicina 29, nr 2 (2014): 97–107. http://dx.doi.org/10.2298/pif1402097j.

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Plum pox virus (PPV) is the causal agent of Sharka disease. Since its discovery, Sharka has been considered as a calamity in plum orchards. PPV is present worldwide in many Prunus species, causing great economic losses. In highly susceptible plum varieties, such as Pozegaca, PPV causes a premature fruit drop and reduces fruit quality, which leads to total yield loss. Eight PPV strains (PPV-M, PPV-D, PPV-EA, PPV-C, PPV-Rec, PPV-W, PPV-T and PPVCR) have been recognized so far. Three major strains (PPV-M, PPV-D and PPV-Rec) are the most widely dispersed and occur frequently in many European countries. Other strains are of minor importance due to their limited host preferences or geographic distribution. So far, all three major strains have been identified in Serbia. In this paper, we provide a comprehensive overview of the research into Plum pox virus variability in Serbia.
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Capote, Nieves, M. Teresa Gorris, M. Carmen Martínez, Margarita Asensio, Antonio Olmos i Mariano Cambra. "Interference Between D and M Types of Plum pox virus in Japanese Plum Assessed by Specific Monoclonal Antibodies and Quantitative Real-Time Reverse Transcription-Polymerase Chain Reaction". Phytopathology® 96, nr 3 (marzec 2006): 320–25. http://dx.doi.org/10.1094/phyto-96-0320.

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The dynamics of virus interference between two isolates of Plum pox virus (PPV) belonging to the main PPV types, D and M, were analyzed in Japanese plum (Prunus salicina) by challenge inoculations. To assess the consequences of a PPV-M infection on plum already infected with PPV-D, and vice versa (predominance of one of the strains, recombination, synergism, symptoms aggravation, and so on), 30 Japanese plum trees were graft inoculated with PPV-D or PPV-M isolates in quarantine conditions. One year postinoculation, in the event that the inoculated isolates were detected in the whole plant, a second challenge inoculation (PPV-M or PPV-D, respectively) was performed by grafting. The presence of PPV-D, PPV-M, or both was monitored for 7 years by double-antibody sandwich indirect enzyme-linked immunosorbent assay using specific monoclonal antibodies. Reverse transcription-polymerase chain reaction (RT-PCR) with D- and M-specific primers confirmed the serological typing. Real-time RT-PCR assays were performed using D- and M-specific fluorescent 3′ minor groove binder-DNA probes, which were able to detect and quantify PPV populations in the inoculated plants with greater precision. The presence of PPV-D in Japanese plum did not cross-protect the trees against PPV-M infection. In PPV-D-infected plants, the PPV-M strain used as challenge inoculum behaved differently depending on the plum cultivar assayed. In cv. Black Diamond, PPV-M invaded the plant progressively, displacing the previous PPV-D population; whereas, in cv. Sun Gold, both PPV isolates coexisted in the plant. In contrast, the PPV-D isolate used was unable to infect plants of both cultivars in which a PPV-M population already was established. After 7 years, no synergism was observed and no recombination event between PPV-D and PPV-M genomes was detected.
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40

Gougherty, A. V., i F. W. Nutter. "Impact of Eradication Programs on the Temporal and Spatial Dynamics of Plum pox virus on Prunus spp. in Pennsylvania and Ontario, Canada". Plant Disease 99, nr 5 (maj 2015): 593–603. http://dx.doi.org/10.1094/pdis-03-14-0224-re.

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The goals of this research were to quantify the impacts of the Pennsylvania and Ontario Plum pox virus (PPV) eradication programs on the spatial and temporal dynamics of PPV epidemics on Prunus spp. The incidence of PPV-positive trees detected over time decreased in both Pennsylvania (−ln 0.85 units per year) and Ontario (−ln 0.51 units per year); however, PPV incidence per year decreased only 60% as fast in Ontario as in Pennsylvania. Marked point pattern analysis revealed that PPV-positive Prunus blocks in Pennsylvania were clustered for distances of 0.7 to 4.3 km, whereas PPV-positive blocks in Ontario were clustered for distances of 1.0 to 25.0 km. Multiyear spatiotemporal analyses revealed that the locations of PPV-positive blocks detected were spatially dependent upon the locations of PPV-positive blocks detected during the previous year. In Pennsylvania, between 2002 and 2006, distances to 95% of newly detected PPV-positive blocks occurred within 10 and 20 km from the previous year’s PPV-positive blocks. In Ontario, 95% of new PPV-positive blocks occurred within 500 to 900 m from PPV-positive blocks detected the previous year. Until 2011, the PPV eradication policy for Prunus blocks in Ontario was triggered by a threshold incidence or PPV-positive tests for three consecutive years. Although the Ontario program was highly successful in reducing PPV incidence from 2001 to 2008, PPV was not eradicated. This study provides important quantitative information concerning the impact of PPV eradication programs on the spatial and temporal dynamics of PPV epidemics in Pennsylvania and Ontario. The analyses employed in this study may help to improve the effectiveness of present and future PPV eradication programs.
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41

Hajizadeh, Mohammad, Adrian J. Gibbs, Fahimeh Amirnia i Miroslav Glasa. "The global phylogeny of Plum pox virus is emerging". Journal of General Virology 100, nr 10 (1.10.2019): 1457–68. http://dx.doi.org/10.1099/jgv.0.001308.

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The 206 complete genomic sequences of Plum pox virus in GenBank (January 2019) were downloaded. Their main open reading frames (ORF)s were compared by phylogenetic and population genetic methods. All fell into the nine previously recognized strain clusters; the PPV-Rec and PPV-T strain ORFs were all recombinants, whereas most of those in the PPV-C, PPV-CR, PPV-CV, PPV-D, PPV-EA, PPV-M and PPV-W strain clusters were not. The strain clusters ranged in size from 2 (PPV-CV and PPV-EA) to 74 (PPV-D). The isolates of eight of the nine strains came solely from Europe and the Levant (with an exception resulting from a quarantine breach), but many PPV-D strain isolates also came from east and south Asia and the Americas. The estimated time to the most recent common ancestor (TMRCA) of all 134 non-recombinant ORFs was 820 (865–775) BCE. Most strain populations were only a few decades old, and had small intra-strain, but large inter-strain, differences; strain PPV-W was the oldest. Eurasia is clearly the ‘centre of emergence’ of PPV and the several PPV-D strain populations found elsewhere only show evidence of gene flow with Europe, so have come from separate introductions from Europe. All ORFs and their individual genes show evidence of strong negative selection, except the positively selected pipo gene of the recently migrant populations. The possible ancient origins of PPV are discussed.
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42

Prikhodko, Yu N., T. S. Zhivaeva i Yu A. Shneyder. "Screening methods to identify the complex of strains of Plum pox virus (PPV)". Horticulture and viticulture, nr 1 (24.03.2019): 36–42. http://dx.doi.org/10.31676/0235-2591-2019-1-36-42.

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From the 10 known strains of thePlumpox virus (PPV) in the Russian Federation 7 strains are spread – D, M. Rec, W, C, CV, and CR with the prevalence of D and W strains. A characteristic feature of the Plum pox virus population in the Russian Federation is the wide spread of W, C and CR strains, extremely rare or not found at all in other regions of the world. Screening tests for detection of the complex of PPV strains by enzyme-linked immunosorbent assay (ELISA) and polymerase chain reaction (PCR) were carried out. It has been established that effective detection of PPV-D, PPV-M, PPV-Rec, PPV-C, PPV-CR, PPV-EA, PPV-W and PPV-T strains is achieved using PPV-ELISA test systems with polyclonal antibodies from ACD, Adgen, Agdia, Bioreba, DSMZ and Loewe companies. Monoclonal antibodies 5B-IVIA do not allow to diagnose isolates related to the strain PPV-CR. For universal detection of isolates of all PPV strains, it is necessary to use a real-time PCR kit for PPV (Syntol), a FLASH-PCR kit for PPV (Agrodiagnostica) or conventional PCR with one of the following primer pairs: P1/P2, s1/as2 or 3´NCP sense/3´NCP antisense.
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43

Gospodaryk, Alina, Inga Moročko-Bičevska, Neda Pūpola i Anna Kāle. "Occurrence of Stone Fruit Viruses in Plum Orchards in Latvia". Proceedings of the Latvian Academy of Sciences. Section B. Natural, Exact, and Applied Sciences 67, nr 2 (1.08.2013): 116–23. http://dx.doi.org/10.2478/prolas-2013-0018.

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To evaluate the occurrence of nine viruses infecting Prunus a large-scale survey and sampling in Latvian plum orchards was carried out. Occurrence of Apple mosaic virus (ApMV), Prune dwarf virus (PDV), Prunus necrotic ringspot virus (PNRSV), Apple chlorotic leaf spot virus (ACLSV), and Plum pox virus (PPV) was investigated by RT-PCR and DAS ELISA detection methods. The detection rates of both methods were compared. Screening of occurrence of Strawberry latent ringspot virus (SLRSV), Arabis mosaic virus (ArMV), Tomato ringspot virus (ToRSV) and Petunia asteroid mosaic virus (PeAMV) was performed by DAS-ELISA. In total, 38% of the tested trees by RT-PCR were infected at least with one of the analysed viruses. Among those 30.7% were infected with PNRSV and 16.4% with PDV, while ApMV, ACLSV and PPV were detected in few samples. The most widespread mixed infection was the combination of PDV+PNRSV. Observed symptoms characteristic for PPV were confirmed with RT-PCR and D strain was detected. Comparative analyses showed that detection rates by RT-PCR and DAS ELISA in plums depended on the particular virus tested. The results obtained in this study revealed that commonly grown plum cultivars in Latvia are infected with economically important stone fruit viruses and highlight the need to implement a programme to produce and propagate virus-free planting material.
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44

Nowak-Wegrzyn, Anna, Jerry A. Winkelstein, Andrea J. Swift i Howard M. Lederman. "Serum Opsonic Activity in Infants with Sickle-Cell Disease Immunized with Pneumococcal Polysaccharide Protein Conjugate Vaccine". Clinical Diagnostic Laboratory Immunology 7, nr 5 (1.09.2000): 788–93. http://dx.doi.org/10.1128/cdli.7.5.788-793.2000.

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ABSTRACT Pneumococcal infections are an important cause of morbidity and mortality in children with sickle-cell disease (SCD). Pneumococcal conjugate vaccines (PCVs) are immunogenic in healthy infants <2 years of age but have not been evaluated in young children with SCD. Infants with SCD were immunized with a 7-valent PCV (Wyeth-Lederle Vaccines & Pediatrics) at 2, 4, and 6 months of age. A booster dose of 23-valent pneumococcal polysaccharide vaccine (PPV; Pnu-Immune) was administered at 24 months of age. Antipneumococcal type 6B and 14 serum opsonic activity was measured to assess the biologic function of the antibody. Following the administration of three doses of PCV, opsonic activity against serotype 6B increased from 4.8% at 2 months to 33.5% at 7 months, with a subsequent decline to 8.1% at 12 months and 7.5% at 24 months and with an increase to 30.7% at 25 months after administration of a booster dose of PPV. Similar trends were seen with serotype 14 (opsonic activities were 9.4% at 2 months, 24.9% at 7 months, 16.5% at 12 months, and 12.6% at 24 months, and the opsonic activity was 27.3% 1 month after the administration of PPV). Serum opsonic activity correlated with antibody levels for both serotypes. PCV induces serum opsonic activity in infants with SCD. Antipneumococcal serum opsonic activity correlates with antibody levels.
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45

Scorza, Ralph, Laurene Levy, Vern Damsteegt, Ann Callahan, Kevin Webb i Michel Ravelonandro. "Transfer of Plum Pox Virus Coat Protein Genes from a Plum Pox-resistant Transgenic Clone of Prunus domestica Plum to Its Progeny through Hybridization". HortScience 33, nr 3 (czerwiec 1998): 532a—532. http://dx.doi.org/10.21273/hortsci.33.3.532a.

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Sharka or plum pox virus (PPV) is a major disease of stone fruit and causes severe economic losses in Europe. There is little resistance to PPV in most Prunus species, thus genetic engineering represents a potentially useful approach to obtain resistant germplasm. Transgenic plums containing the PPV coat protein (CP) or the related papaya ringspot virus (PRV)-CP gene were produced through Agrobacterium tumefaciens-mediated transformation. These transgenic plum clones were then evaluated for resistance to PPV infection in the greenhouse by graft or aphid inoculation with PPV. While symptoms of PPV appeared in most transgenic clones, all plants of PPV-CP transgenic clone C5 were symptomless and ELISA and immunocapture-reverse transcriptase PCR negative for over three years following inoculation with two strains of PPV (Ravelonandro et al., Plant Dis. 81:1231-1235, 1997). Clone C5, which contains multiple copies of the PPV-CP gene, was hybridized with PRV-CP transgenic plants or untransformed plum cultivars. Progeny were obtained containing no transgenes, only the PPV-CP, only the PRV-CP, or both the PRV-CP and PPV-CP transgenes. Seedlings were inoculated with PPV. At 5 and 11 months post-inoculation, seedlings containing the PPV-CP genes from C5 were symptomless and ELISA negative. Seedlings containing only PRV-CP transgenes or non-transformed controls showed symptoms of PPV infection and were ELISA positive. These results indicate that the PPV-CP transgenes can be transferred to progeny through hybridization and that these genes can impart resistance to PPV in transgenic seedlings. The inheritance of the multicopy inserts of the PPV-CP and PRV-CP transgenes is being analyzed. The combined effects of both transgenes on resistance to PPV and the stability of PPV resistance in the progeny of the resistant C5 transgenic line are currently under evaluation.
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46

Kamenova, I., S. Dallot, V. Bozkova i S. Milusheva. "First Report of the Plum Pox Virus Recombinant Strain on Peach in Bulgaria". Plant Disease 95, nr 10 (październik 2011): 1320. http://dx.doi.org/10.1094/pdis-05-11-0405.

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Plum pox virus (PPV) causes sharka, the most damaging viral disease of stone fruit species. Seven distinct PPV strains are known; PPV-M, PPV-D, and PPV-Rec are the most common (3). PPV-Rec is a unique recombinant (3) between PPV-M and PPV-D and has been reported from plum, apricot, Japanese plum, myrobalan, and blackthorn in eastern and central Europe, but has never been found in peach as a single natural infection (2). A survey was conducted during spring 2009 in eight peach orchards located in the southwest, southeast, and south central regions of Bulgaria to assess the incidence of PPV infection. A total of 98 leaf samples from individual trees showing PPV-like symptoms were collected and analyzed by triple-antibody sandwich (TAS)-ELISA with the universal monoclonal antibody (MAb) 5B (Agritest, Valenzano, Italy). Sixty one samples reacted positive for PPV (optical density 0.161 to 1.267) and these samples were further analyzed with PPV-M (AL) and PPV-D (4DG5) specific MAbs (1). All 61 samples reacted positively with PPV-M specific MAbs. To distinguish PPV-M and PPV-Rec strains, which are serologically identical, immunocapture (IC)-reverse transcription (RT)-PCR was carried out with PPV-M (CIP-M: 5′-GTC GCA GCA TTT GTA GCC CTT GTT-3′, CIP-MR: 5′-CCA ACA CGT TAA CGC CAT GCT TCA-3′) and PPV-D (CIP-D: 5′-ATG ATG CTG TTT GAC TCG GAG CGA-3′, CIP-DR: 5′-TCG CAA CTG CTT GCA CAC ATT CTC-3′) specific primers targeting the 6K1-CI genomic region. A PCR fragment of ~880 bp amplified with PPV-M specific primers obtained from 59 samples confirmed that these were PPV-M isolates. However, the remaining two samples (both coming from infected tress located in two different orchards in the southwest region) yielded a 468-bp PCR fragment with PPV-D specific primers, suggesting that these two samples belonged to PPV-Rec strain. These samples together with controls of PPV-M, PPV-D, and PPV-Rec strains were further analyzed by RT-PCR using mD5/mM3 primers spanning the recombination breakpoint (4). Both peach samples and the PPV-Rec strain control produced a single 605-bp PCR product. The two peach amplicons were purified and sequenced directly with the same primers. The nucleotide (nt) sequences obtained were 100% identical to each other. BLAST analysis of the two samples with PPV-Rec (No. AF421118.1) showed maximum nt identity of 98%. Percent maximum nt identity with PPV-M (No. AY324837.1) and PPV-D (No. AB576062.1) were 93 and 87%, respectively. The deduced amino acid sequences of the two isolates were 98% identical to PPV-Rec (No. No. AF421118.1), 93% identical to PPV-M (No. M92280.1), and 84% identical to PPV-D (No. AB576062.1). Analyzed samples were further transmitted from the diseased trees to peach seedlings (GF 305) by chip-budding in a greenhouse during the fall of 2009. Six months later, faint vein clearing on the leaves of inoculated seedlings was observed. The presence of PPV was confirmed by TAS-ELISA and PPV-Rec presence was shown by IC-RT-PCR (mD5/mM3 primers). One of the generated 605-bp products was sequenced and showed 100% nt identity with the isolate used for inoculation. To our knowledge, this is the first identification of PPV-Rec strain in naturally infected peach trees, a finding that calls for further large-scale investigations of PPV-Rec incidence in peach in Bulgaria. References: (1) M. Cambra et al. OEPP/EPPO Bull. 24:569, 1994. (2) S. Dallot et al. Acta Hortic. 781:227, 2008. (3). M. Glasa et al. J. Gen. Virol. 85:2671, 2004. (4) Z. Šubr et al. Acta Virol. 48:173, 2004.
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47

Dagadu, Nii Amah, Shahram Ajori, Yaw Delali Bensah, Kwabena Kan-Dapaah, Stephen Kofi Armah, Boateng Onwona-Agyeman i Abu Yaya. "Stacking Interactions of Poly Para-Phenylene Vinylene Oligomers with Graphene and Single-Walled Carbon Nanotubes: A Molecular Dynamics Approach". Molecules 25, nr 20 (20.10.2020): 4812. http://dx.doi.org/10.3390/molecules25204812.

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This study is meant to address the understanding of the interactions between poly para-phenylene vinylene (PPV) oligomers, graphene and single-walled carbon nanotubes (SWCNT). To this end, the binding energies of the PPV oligomers with graphene and SWCNTs were investigated. Calculations are performed and the parameters related to van der Waal vdW interactions are discussed to achieve and confirm the crystallization of oligomers of PPV into herringbone (HB) structure arrangement, which is known to be the most stable conformation at 300 K. Finally, the interfacial interactions between crystal PPV, graphene and SWCNT are carried out. According to the results, the intramolecular potential energies of PPV chains are found to increase linearly with each extending PPV monomer unit by approximately 50 kcal/mol. Moreover, the interfacial interaction properties analysis using radial distribution functions (RDFs) for PPV-graphene and PPV-SWCNT show significant disordering of the arrangement of molecules, which is more pronounced for PPV-SWCNT than that in PPV-graphene. The radius of gyration (Rg) profiles show a net decrease of ∼−0.8, for PPV-graphene with different surface coverage, and, a net increase of ∼+0.6, for PPV-SWCNT; meaning that, the binding between PPV-graphene is much stronger than with PPV-SWCNT.
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48

Polák, J., J. Pívalová i J. Svoboda. "Prune cv. Jojo resistance to different strains of Plum pox virus". Plant Protection Science 41, No. 2 (23.02.2010): 47–51. http://dx.doi.org/10.17221/2742-pps.

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Trees of prune (<i>Prunus domestica</i> L.), cv. Jojo, were inoculated by chip budding with three different strains of PPV isolated from European plum in the Czech Republic. These isolates included Plum pox virus M strain (PPV-M), <i>Plum pox virus</i> D strain (PPV-D) and a PPV-recombinant both strains (PPV-Rec). The results of the evaluation of the inoculated trees over 2 years are presented. Trees of plum cv. Jojo behaven differently to infection with the three PPV strains. A strong hypersensitive reaction appeared a year after inoculation with PPV-M and PPV-Rec strains, although not all inoculated tree died. PPV must have been present in the tissue of cv. Jojo because the virus was transferred to the rootstock St. Julien. Plants of the rootstock became systemically infected with the PPV-M and PPV-Rec strains, showing severe PPV symptoms. The presence of PPV was proved by ELISA in leaves of rootstock St. Julien, but not in leaves of cv. Jojo. Inoculation with strain PPV-D resulted in partial hypersensitive reaction of plants of cv. Jojo, but after initial stunting and partial death of shoots recovering of plants was observed.
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49

Moinuddin, Omar, Rebhi O. Abuzaitoun, Min W. Hwang, Sanjana K. Sathrasala, Xing D. Chen, Joshua D. Stein, Mark W. Johnson, David N. Zacks, Thomas J. Wubben i Cagri G. Besirli. "Surgical repair of primary non-complex rhegmatogenous retinal detachment in the modern era of small-gauge vitrectomy". BMJ Open Ophthalmology 6, nr 1 (luty 2021): e000651. http://dx.doi.org/10.1136/bmjophth-2020-000651.

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ObjectiveTo report anatomic and visual outcomes of pars plana vitrectomy (PPV), as well as scleral buckling (SB) and PPV/SB as surgical treatments for the management of primary, non-complex rhegmatogenous retinal detachment (RRD).Methods and analysisData from 751 eyes that underwent PPV, SB or combined PPV/SB as a surgical treatment for primary non-complex RRD with at least 3 months of follow-up were analysed to determine rates of single surgery anatomic success (SSAS) and final anatomic success (FAS). Patients or the public were not involved in the design, conduct or reporting of this research.ResultsPPV accounted for 89.0% (n=668), PPV/SB for 6.8% (n=51) and SB for 4.2% (n=32) cases. Overall SSAS (91.2% PPV, 84.3% PPV/SB, 93.8% SB; p=0.267) and FAS (96.7% PPV, 94.1% PPV/SB and 100.0% SB; p=0.221) were reported for the three surgical groups. SSAS and FAS were similar for lens status, macular detachment status and the presence or absence of inferior retinal breaks for each of the PPV, PPV/SB and SB groups.ConclusionsIn this large, single institution, retrospective case series, we report surgical outcomes for patients with primary non-complex RRD managed with PPV, SB or PPV/SB in the modern era of small-gauge vitrectomy. We demonstrate that primary PPV without adjunct SB provides excellent anatomic and visual outcomes irrespective of lens status, macular involvement or pathology location.
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50

Zaquen, N., K. Verstraete, L. Lutsen, D. Vanderzande i T. Junkers. "Modifiable poly(p-phenylene vinylene) copolymers towards functional conjugated materials". Polymer Chemistry 7, nr 29 (2016): 4771–81. http://dx.doi.org/10.1039/c6py00688d.

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The copolymerization of poly[2-methoxy-5-(3,7-dimethyloctyloxy)-p-phenylene vinylene] (MDMO-PPV) with poly[2-methoxy-5-(carboxypentyloxy)-PPV] (CPM-PPV) and poly[2,5-dicyano-PPV] (CN-PPV) is studied and postpolymerization modification with water soluble substituents is demonstrated.
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