Gotowa bibliografia na temat „Pinus radiata Soils”

Phosphorus (P) availability was investigated in rhizosphere soils under 4- to 5-year-old, second-rotation Pinus radiata D. Don and understorey grass (browntop, Agrostis capillaris L.) in two P-deficient Andosols (a Pumice Soil and an Allophanic Soil). Pinus radiata rhizosphere had more soil organic matter, greater mycorrhizal hyphal length density, higher acid phosphatase activity, and more concentrated dissolved organic carbon than bulk soil or the rhizosphere of grass species in one or both soils. Concentrations of resin P and organic P in the P. radiata rhizosphere were higher than those in the grass rhizosphere and bulk soils, suggesting that P. radiata rhizosphere processes have a greater potential to accumulate organic P and mobilize soil P than understorey grass rhizosphere processes. This effect was less marked in the Allophanic Soil than in the Pumice Soil, probably because of the higher P-fixing capacity and lower plant-available P concentrations in the Allophanic Soil.

The effects of radiata pine (Pinus radiata D. Don) seedlings and ryegrass (Lolium perenne L.) on the mineralisation of orthophosphate monoesters in 7 grassland soils were assessed in a 10-month pot trial using NaOH–EDTA extraction and solution 31P NMR spectroscopy. Extraction with NaOH–EDTA recovered 46–86% of the total soil P, and NaOH–EDTA-extractable organic P determined by molybdate colourimetry ranged between 194 and 715 mg/kg soil, representing 34–85% of the total soil organic P. Orthophosphate monoesters were the predominant species of the extracted organic P in all soils, with much smaller concentrations of orthophosphate diesters, and traces of phosphonates. Concentrations of orthophosphate monoesters were consistently lower in soils under pine (103–480 mg P/kg soil) compared with the initial soils (142–598 mg P/kg soil) and most soils under grass (122–679 mg/kg soil). Mineralisation of myo-inositol hexakisphosphate accounted for 18–100% of the total mineralisation of orthophosphate monoesters in most soils under radiata pine. This suggests that supposedly recalcitrant inositol phosphates are available for uptake by radiata pine, although the extent of this varies among soils.

Soil profile descriptions were made at a series of 11-year-old unfertilized Pinus radiata stands in the Lithgow district of New South Wales. Catenas within three soil parent materials were selected to compare variation in soil physical and morphological properties with growth of P. radiata. These parent materials were a Silurian siltstone, a Permian conglomerate and a Silurian-Devonian rhyolite. Basal area growth of the P. radiata stands increased down catenas on the Silurian siltstone as soil depth to a root impeding layer increased. Plateau soils on the Permian conglomerate had hardsetting surfaces and high gravel contents, and were associated with very poor pine growth. By way of contrast, lower slope, colluvial gradational earths were deep, fine-textured soils and supported more productive pine stands. The Silurian-Devonian rhyolite parent material produced highly leached soils, commonly with conspicuously bleached A2 horizons and poor sandy textures of surface soil. Both physical and chemical features of the rhyolite interacted with pedological processes to affect adversely soil physical conditions and trace element availability, in particular boron. The poorer P. radiata growth on lower or concave slope in comparison with upper slope position was a result of increased soil leaching and horizon differentiation. This pattern contrasted with improved pine growth on the deeper soils on lower slopes on the two sedimentary parent materials. These case studies emphasize the importance of geology and pedological processes when evaluating the applicability of specific soil physical factors to site classification for P. radiata plantations.

To understand the effects of agroforestry on soil biological processes we assessed the conditions in Pinus radiata plantations of 50, 100, 200, and 400 stems/ha after 25 years of growth, and in a grassland. Agroforestry resulted in a 15–25% decline in soil organic C and N compared with grassland, and had a significant negative influence on soil microbial biomass. There was less microbial C and N in soils under 50–400 stems/ha of P. radiata than in soils under grassland (0 stems/ha). Soil carbon decomposition and microbial activity were measured by trapping the carbon dioxide produced by incubating soils over a 60-week period. The results showed that soil C decomposition rates were ~1.5 times as much (c. 15 mg CO2-C/kg soil) in soil from grassland as in that from plots with 50 or100 stems/ha (c. 10 mg CO2-C/kg soil), and were further reduced to one half (c. 5.5 mg CO2-C/kg soil) in the plots with 200 or 400 stems/ha. The soils under P. radiata gave off less carbon dioxide per unit of biomass (the metabolic quotient) than soils under grassland. These shifts in microbial biomass and its metabolic quotients appear to be associated with differences in the quantity and ‘quality’ of inputs and soil organic matter decomposition rates, and to reflect the land use change from grassland to forest. Given the general ability of soil microbial biomass to recolonise depopulated areas after tree harvest, we see no problem in restoring populations of these soil organisms vital in controlling nutrient cycling after tree felling, provided adequate adjustments to soil pH are made.

Application of Mg fertilisers has been suggested as a means of reducing the incidence of Mg deficiency of forest trees in New Zealand and Europe. The objective of this study was to determine the rates of dissolution of a range of Mg fertilisers applied to a pumice soil (Typic Udivitrand). The rate of fertiliser dissolution was little influenced by whether the fertiliser was applied directly on to the soil surface (litter removed) or on to the litter layer in a Pinus radiata plantation. Twenty-seven months since fertiliser application the mean (with and without litter) percentage of Mg dissolved was in the sequence: Epsom salts > calcined magnesite 1–2 mm > granmag (a partially acidulated and granulated calmag product) > calcined magnesite 2–4 mm > forestry grade dolomite. The specific dissolution rate constants (mg/cm2 .day of fertiliser) for the slowly soluble Mg fertilisers calculated using an elemental sulfur oxidation cubic model were 587 for calcined magnesite 1–2 mm, 426 for calcined magnesite 2–4 mm, 385 for granmag, and 18 for forestry grade dolomite. In a laboratory incubation study the elemental sulfur oxidation cubic model described the rate of dissolution of Mg fertilisers within narrow fertiliser particle size ranges. The specific fertiliser dissolution rate constants, however, increased with decreases in particle size, suggesting that the rate of dissolution depends on factors other than surface area when particle sizes varied widely. Slowly soluble, alkaline Mg fertilisers had a significant liming effect on the soil. They were more effective in increasing soil exchangeable Mg than soluble Mg salts over a long-period and therefore, they are better fertilisers for P. radiata.

Pinus radiata stands are reported to accumulate increased N on addition of phosphatic fertilizers. Field and laboratory studies were initiated to determine if addition of superphoshate increases soil N mineralization in forest soils. In a field experiment, application of 200 kg P ha-1 as superphosphate to trenched plots in a Pinus radiata stand increased accumulated soil mineral-N contents by 122% and 82% above the control on two occasions. Application of 500 kg P ha-1 as superphosphate either alone or in combination with lime (10 Mg ha-1) increased in situ soil net N mineralization in a dry sclerophyll eucalypt forest from 20.7 (control) to 28.3 (+P) and 30.2 (+P+lime) kg N ha-1 yr-1 20 cm-1. Uptake by the vegetation accounted for all of the mineralized nitrogen. In a 180 day laboratory incubation using undisturbed soil columns (0-20 cm) from nine forest sites, seven soils showed a positive response to superphosphate (100 kg P ha-1) addition, with increases in N mineralization ranging from 14% to 117%. The response of N mineralization to superphosphate addition was not related to initial soil organic C, total N or P content in the 0-20 cm layer. However, soil pH and organic C combined to account for 76% of the variation in N mineralization response (P < 0.01). In the 0-5 cm layer of untreated soils, soil organic P content could explain 71% of the variation in net N mineralization. Addition of superphosphate appears to increase N mineralization in several Australian forest soils and the improved availability of N will enhance tree growth rates. The mechanisms underlying the response are still poorly understood and thus also our ability to predict its significance for tree nutrition on specific forest sites.

A technical classification system for soils of Pinus radiata plantations is presented. The soil attributes used were mainly from published data and classes were established on an a priori basis from information and experience of plantation forestry with this species. The system is designed for field use and does not require laboratory analyses; it uses factors that are not affected by the presence of the forest species; the classes are coded so as to produce data suitable for computerized information systems. The attributes used are parent rock, texture profile, depth to and nature of any impeding layer, texture and condition of the uppermost 10 cm of soil, character of surficial horizons according to paler subsurface or exposed subsoil, and the condition and colour of the subsoil. A parent rock classification has been devised according to soil-forming potential; this reduces rock identification to 12 main classes so as to facilitate field recognition. The classification is aimed at assisting soil-based silvicultural management of P. radiata forests, providing a basis for transferring technology between forests, and at accumulating specific resource information for inclusion in forest management information systems.

Chromium is used as Cr(III) in the tannery industry and as Cr(VI) in the timber treatment industry. In this experiment, the effect of 2 liming materials [fluidised bed boiler ash (FBA) and lime] and an organic amendment (Pinus radiata bark) on the retention of Cr by 2 soils (Egmont and Tokomaru) was examined using both ‘batch’ and ‘column’ experiments. The effect of these amendments on the uptake of Cr from the Egmont soil, treated with various levels of Cr (0–3200 mg Cr/kg soil), was examined using sunflower (Helianthus annuus) plants. The transformation of Cr was examined by fractionation of Cr in soils. Addition of FBA and lime increased the retention of Cr(III), but had the opposite effect on the retention of Cr(VI). Addition of bark did not affect the retention of Cr(III), but it increased the retention of Cr(VI). Increasing additions of Cr increased Cr concentration in plants, resulting in decreased plant growth. The liming materials were found to be effective in reducing the phytotoxicity of Cr(III) and the Pinus radiata bark was effective for Cr(VI). In both Cr(III)- and Cr(VI)-contaminated soils, the concentrations of Cr were higher in the organic-bound, oxide-bound, and residual fractions than in the soluble and the exchangeable fractions. The concentrations of Cr in the soluble and the exchangeable fractions were higher in the Cr(VI)-contaminated than the Cr(III)-contaminated soil. Addition of the liming materials decreased the concentration of the soluble Cr(III) and bark decreased soluble Cr(VI) in soil. There was evidence for the reduction of Cr(VI) to Cr(III) in the bark-treated soils.

Boron deficiency in Pinus radiata (D. Don) plantations in N.S.W. has been particularly evident on soils derived from acid igneous parent materials. A slope sequence (catena) of soils on a rhyolitic parent material was selected to study the amount of boron retention and its relationship to soil development. The soils at three positions, a hillcrest, mid-slope and lower slope, were described and various soil chemical and physical properties were determined for each horizon. Tree height, deformity, survival and foliar chemistry of 6 year old P. radiata were measured in plots adjacent to the three soil profiles studied. Boron adsorption isotherms varied within individual soil profiles by horizon and also between the different soil profiles in the catena. Boron adsorption was highest in the hillcrest soil B horizons and lowest in the bleached A2 horizons of the lower slope soil. The Freundlich isotherm constant k was found to be significantly correlated with clay content (r = 0.88), exchangeable aluminium (r = 0.79, exchangeable potassium (r = 0.68), and dithionite-citrate extractable iron (r = 0.66). Leaching and illuviation of iron oxides and clay has been accentuated in the two soil profiles on the hillslope sites of the catena. As clay, aluminium and iron oxides have been removed from the surface horizons of the lower slope soil, the ability of these horizons to adsorb and therefore retain boron has been greatly diminished. Position on the catena also affected P. radiata growth survival and foliar chemistry. Tree height decreased while the incidence of deformity increased down the slope. It is suggested that the progressive deterioration in tree growth down the slope is mainly caused by the trees being increasingly affected by boron stress as a result of decreasing capacity of the soils to retain boron by adsorption against leaching towards the lower slope.

The aboveground and belowground productivity of forest systems are interlinked through complex feedback loops involving tree, soil and environmental factors. With a predicted significant change in environmental conditions through the enhanced greenhouse effect, it is important to understand the response of forest systems to these new conditions. An increase in atmospheric CO2 is predicted to increase photosynthesis, and therefore whole plant productivity at the individual tree level. However this increase in photosynthesis may result in greater requirements for nutrients, particularly nitrogen (N). In order to acquire any additional available N, trees may respond by increasing their proportional allocation of C belowground to the root system. This study aimed to quantify the belowground C allocation in a mature forest system consisting of a single species on a single site, but with different levels of water and nutrient stress. The belowground carbon dynamics of a range of irrigated and fertilized Pinus radiata stands in Australia were investigated during 1992 and 1993. Belowground carbon allocation was estimated using the model proposed by Raich and Nadelhoffer (1989) where belowground C allocation is the difference between soil respiration and carbon input through litterfall, plus coarse root production and an adjustment for any change in soil and litter layer carbon pools. This model is best described by the equation: Belowground C = Csoilresp ?? Clitterfall + Ccoarseroot+ ???Cforest floor+ ???Csoil Soil respiration, measured using a modified soda lime absorption method either every 2 weeks or every 4 weeks for 2 years, showed a range in daily soil C flux from 137 ?? 785 mgCO2.m-2.h-1. Soil respiration showed seasonal trends with summer highs and winter lows. Limited fine root biomass data could not indicate a strong relationship between measured soil respiration and fine root (>2mm diameter) biomass. Fifty three percent of the variation in soil respiration measurements in irrigated treatments was explained by a linear relationship between soil respiration, and soil temperature at 0.10 m depth and litter moisture content. In non-irrigated treatments, 61% of the variation in soil xix respiration measurements was explained by a linear relationship between soil temperature at 1 cm depth and soil moisture content. Inter-year variation was considerable with annual soil respiration approximately 20% lower in 1993 compared with 1992. Annual soil C flux was calculated by linear interpolation and ranged from 3.4 ?? 11.2 tC ha-1 across the treatments. Soil C pools remained unchanged over 10 years between 1983 and 1993 for all combinations of irrigated and fertilized stands, despite significant aboveground productivity differences over the decade. Measurements of standing litter showed a change between 1991 and 1993 for only 2 out of the 10 treatments. These two treatments had belowground C allocation estimated both with and without an adjustment for a change in standing litter. Annual litterfall C ranged almost four fold from 0.6 ?? 2.2 tC ha-1 between the treatments in 1992 and 1993, and fell within the ranges of measured litterfall over 10 years at the field site. Again inter-year variation was large, with the 1993 litterfall values being approximately 97% greater across all treatments compared with 1992 values. Belowground carbon allocation was calculated using C fluxes measured at the field site, and ranged 3 fold from 4.4 ?????? 12.9 tC ha-1 between the treatments during 1992 and 1993. In 1993 the belowground C allocation was approximately 30% lower across all treatments compared with 1992 calculations. This was due to an approximate 23% reduction in annual soil C flux, a 97% increase in litterfall C and an 18% reduction in coarse root production between 1992 and 1993. The field site was N limited, and differences in belowground C allocation could be shown across irrigated treatments with different N limitations. As N availability increased belowground C allocation was decreased in the irrigated treatments. It was difficult to determine differences in belowground C allocation caused by water stress as the effects of water and N limitation were confounded. An increase in N availability generally indicated an increase in coarse root and litterfall C production, which were reflected in increased aboveground productivity. In high N treatments the coarse root fraction of belowground C allocation comprised approximately 50% of the total belowground C allocation, whereas in the N stressed treatments coarse roots only comprised 20% of the total belowground allocation The mechanistic model BIOMASS was used to estimate annual gross primary productivity (GPP) for the different treatments at the field site. BIOMASS estimated GPPs of between 30-38 tC ha-1 for the different treatments during 1992 and 1993. The measured belowground carbon allocation ranged from 16 ?? 40 % of simulated GPP, with the lower proportion allocated belowground in the irrigated and high fertility stands. Aboveground competition through the absence of thinning also appeared to reduce allocation belowground in non- irrigated stands. A direct trade off between bole and belowground C could not be demonstrated, unless data were separated by year and by the presence or absence of irrigation. Where data were separated in this manner, only three data points defined the reasonably strong, negative relationship between bole and belowground C. The value of this relationship is highly questionable and should be interpreted with caution. Thus a decrease in belowground C allocation may not necessarily indicate a concomitant increase in bole C allocation. Inter-year variation in a number of C pools and fluxes measured at the field site was at least as great as the variation between stands having different water and N limitation. Extrapolation of belowground productivity estimates from a single years data should be undertaken cautiously. The work undertaken in this study indicated that for a given forest stand in a given soil type, an increase in N availability reduced the absolute and relative C allocated belowground. However this decrease in C belowground may not directly translate as an increase in stem growth or increased timber production. Forest productivity in an enhanced greenhouse environment is likely to result in an increased allocation of C belowground due to increased N limitation, unless adequate N is present to support a more active canopy. Further work is required to more fully understand the dynamics of the belowground system in a changing environment. However further research should focus on mature forest systems in order to isolate the impacts of natural ageing changes from perturbation effects on the forest system. This would be best undertaken in long term monitoring sites where a C history of the stand may be available.

A comparison of above ground forest metrics with below ground soil CO₂ respiration was carried out in an attempt to reveal if any correlations exist. Above ground measurements of 2720 clonally propagated trees were taken assessing the silvicultural treatments of stocking, herbicide and fertiliser. These were compared to 480 below ground soil CO₂ respiration measurements. Using measurements of mean height, mean dbh and basal area the data was analysed and returned significant results for mean dbh and the interactions of herbicide and clones, and stocking and herbicide. Mean height returned a significant result for the interaction of stocking and herbicide. Below ground measurements showed an interaction between ripping and stocking; however these results were not ratified by the above ground results. Overall the results were encouraging and should aid in future experiments that seek to understand what effect above ground treatments have on below ground CO₂ activity.

Thesis (MSc (Forest and Wood Science))--University of Stellenbosch, 2011.
ENGLISH ABSTRACT: This study investigated the use of carbon isotopes as a potential measure for water availability and drought stress in Pinus radiata in the Western and Southern Cape, South Africa. An understanding of water availability and its variation in space is fundamental to the implementation of increasingly site-specific management regimes that have the potential to greatly improve productivity across sites in the region. Fifteen plantation compartments situated on water shedding sites were identified where good weather data existed and a water balance model could be run. In addition, late wood samples were analysed from four co-dominant trees in the same stand to determine the δ13C values of five tree rings, each representing a specific growth year before first thinning. Detailed water balances were constructed for each trial site and drought stress indicators (a) relative canopy conductance (after Granier et al., 2000) and (b) the ratio of actual to potential evapotranspiration (supply / demand ratio), were related to δ13C values in latewood. Maximum available soil water ranged from 52 to 313 mm across trial sites. The water balance model used adequately described soil water availability throughout each growing season and indicated that stand stress due to the lack of available soil water mainly occurred during the summer months of the study period (November to April). The supply / demand ratio for this period as well as the relative canopy conductance proved to be good measures of drought stress. The six-month supply demand ratio (calculated for the period November to April) ranged from 0.04 to nearly 1 (winter rainfall zone) and 0.35 to 1 (all-year rainfall zone) and were strongly related to δ13C values (p < 0.001; r2 = 0.7822). It appears that using δ13C values, it may be possible to classify sites into three water availability classes. This classification may assist in the implementation of intensive silvicultural operations on an increasingly site-specific basis. Where sites are enriched with water from lateral flow or upslope positions, δ13C may be the only reliable technique to quantify soil water availability.
AFRIKAANSE OPSOMMING: Hierdie studie ondersoek die gebruik van koolstof isotope as 'n moontlike maatstaf vir die beskikbaarheid van water en droogtestremming in Pinus radiata in die Wes-en Suid-Kaap, Suid-Afrika. 'n Begrip van die beskikbaarheid van water en die ruimtelike variasie daarvan is fundamenteel vir die implementering van groeiplek-spesifieke bestuur sisteem wat die potensiaal het om baie verbeterde produktiwiteit oor persele in die streek teweeg te bring. Vyftien plantasievakke, geleë op waterskeidingsterreine is geïdentifiseer waar goeie weer data bestaan en 'n water balans model uitgevoer kon word. Daarmee saam is laathout monsters vanuit vier ko-dominante bome in dieselfde kompartement geanaliseer en die δ13C waardes van laathout in vyf jaarringe bepaal wat elk 'n spesifieke jaar van groei voor die eerste dunning verteenwoordig. Gedetailleerde water balanse is vir elke proef perseel bereken en aanwysers van droogtestremming, nl.: (a) relatiewe kroon geleiding (na Granier et al., 2000) en (b) die verhouding van die werklike teenoor potensiële evapotranspirasie (vraag / aanbod verhouding) is gekorreleer met 13C waardes in laat hout. Die maksimum hoeveelheid water beskikbaar op die verskeie proefpersele wissel van 52 tot 313 mm. Die water balans model wat gebruik is beskryf die beskikbare grondwater met genoegsame akkuraatheid. vir die hele groeiseisoen. Die model dui ook aan dat die kompartemente droogtestremming as gevolg van die gebrek aan beskikbare grond water ervaar gedurende die somer maande van die studie tydperk (November tot April). Die vraag / aanbod verhouding vir hierdie tydperk, asook die relatiewe kroon geleiding is geskik om as maatstawwe van droogtestremming gebruik te word. Die vraag / aanbod verhouding (bereken vir die tydperk November tot April) het gewissel van 0,04 tot byna 1 (Winter reënval gebied) en 0,35 tot 1 (die heel jaar reënval sone) en is sterk verwant aan 13C waardes (p <0,001; r2 = 0,7822). Dit blyk dat met die gebruik van δ13C waardes, dit moontlik kan wees om kompartemente te klassifiseer in drie klasse van water beskikbaarheid. Hierdie klassifikasie kan help met die implementering van intensiewe boskultuur bedrywighede op 'n meer vak-spesifieke basis. Waar vakkeverryk is met water vanuit laterale vloei of hoër liggende posisies, mag δ13C dalk die enigste betroubare tegniek wees om die beskikbaarheid van water te kwantifiseer.

This thesis investigates the abiotic control of carbon (C) and water vapour fluxes (FCO₂ and E, respectively) in a New Zealand Pinus radiata D. Don plantation and a nearby clearcut. It concentrates on the limitation of these fluxes imposed by growing season soil water deficit. This results from low precipitation (658 mm a⁻¹) in combination with a limited root zone water storage capacity of the very stony soil (> 30% by volume). The thesis analyses results from seven eddy covariance flux measurement campaigns between November 1994 and March 1996. The study site was located in Balmoral Forest, 100 km north-west of Christchurch (42° 52' S, 172° 45' E), in a (in November 1994) 8-year-old stand. One set of measurements was conducted in an adjacent clearcut. Ecosystem flux measurements were accompanied by separate measurements of ground fluxes and of the associated environmental variables. Flux analysis focussed on the underlying processes of assimilation (Ac), canopy stomatal conductance (Gc) and respiration (Reco), using biophysical models coupled to soil water balance and temperature subroutines. Aiming to link time inegrated net ecosystem C (NEP) to tree growth, sequestration in tree biomass (NPP) was quantified by regular measurements of stem diameter using allometric relationships. Average rates of FCO₂ and E were highest in spring (324 mmol m⁻² d⁻¹ and 207 mol m⁻² d⁻¹, respectively) when the abiotic environment was most favourable for Gc and Ac. During summer, fluxes were impeded by the depletion of available soil water (θ) and the co-occurrence of high air saturation deficit (D) and temperature (T) and were equal or smaller than during winter (FCO₂ = 46 mmol m⁻² d⁻¹ in summer and 115 mmol m⁻² d⁻¹ in winter; E = 57 and 47 mol m⁻² d⁻¹, respectively). With increasingly dry soil, fluxes and their associated ratios became predominantly regulated by D rather than quantum irradiance, and on particularly hot days the ecosystem was a net C source. Interannually, forest C and water fluxes increased strongly with rainfall, and the simultaneously reduced D and T. For two succeeding years, the second having 3 % more rain, modelled NEP was 515 and 716 g C m⁻² a⁻¹, Ac 1690 and 1841 g C m⁻² a⁻¹ and Reco 1175 and 1125 g C m⁻² a⁻¹. NEP / E increased in wetter (and cooler) years (1.3 and 1.5 g kg⁻¹), reflecting a relatively larger gain in NEP. Responding mainly to increased rainfall during commonly dry parts of the year (ie summer), and reflecting the otherwise benign maritime climate of New Zealand, NEP during the winter months could exceed NEP during the middle of the notional tree growing season. Annual Ac, NEP, and NPP were strongly linearly related. This relation did not hold during bi-weekly periods when the processes of intermediate C storage were influential. Separate knowledge of tree growth and C fluxes allowed quantification of autotrophic, and heterotrophic respiration (Rhet≈ 0.4 NEP), as well as fine-root turnover (≈0.2 NEP). The ratio of NEP and stem volume growth was conservative (0.24 t C m⁻³) and allows a direct connection to be made between ecosystem carbon fluxes and forest yield tables. In the absence of living roots, the clearcut flux measurements demonstrated the expected limitation of Rhet by soil temperature (Ts) and θ. However, an additional 'pumping effect' was discovered at the open site whereby turbulence increased CO₂ efflux considerably when the soil surface was wet. Accounting for the combined effects of Ts, θ and turbulence, annual Rhet at the clear-cut site (loss to the atmosphere) was »50 % of NEP (C sequestered from the atmosphere) in the nearby forest. Clearly, there is an important contribution of C fluxes during early stages of ecosystem development to the total C sequestered over the lifetime of a plantation.

Thesis (MscFor)--Stellenbosch University, 2011.
ENGLISH ABSTRACT: The purpose of the study was to investigate the effect of mid rotation fertilizer application on leaf area index (LAI), basal area and volume increment in thinned Pinus radiata stands on the most common soils of the Boland region in the Western Cape. The study was conducted on a range of sites in the Boland region of MTO Forestry Company, chosen to reflect the two most common soil types and a water availability gradient in each soil type. A factorial combination of fertilizer treatments with three levels each for nitrogen (N) at 0, 100 and 200 kg ha-1 and phosphorus (P) at 0, 50 and 100 kg ha- 1 was used. This design was replicated four times across a gradient of water availability for each of the two common soil groups, forming a complete trial series. All replications were laid out in P. radiata stands that had received their mid-rotation thinning prior to treatment implementation. LAI, diameter at breast height and height measurements as well as foliar analysis were determined before the implementation of the study in 2008 and then subsequently at predetermined intervals in 2009 and 2010. Leaf area index and stem volume increment were measured in order to evaluate the influence on growth efficiency. LAI was estimated using the gap fraction method with the use of a ceptometer. Volume increment was calculated using diameter and height measurements and basal area was calculated by means of diameter measurements. The abovementioned growth responses were then used to determine the effect of increased nutrient availability on stand growth. There were no significant interactions detected between any of the factors, N, P and water availability class in their effect on LAI, basal area, volume increment and growth efficiency. LAI increment responded significantly to N and P in the first year but only to P in the second year after treatment. Significant basal area responses to N and P were recorded in the second but not the first year. This might have been due to the fact that trees had to re-build their canopies after thinning before a basal area response could be obtained. For the variables where an analysis of total growth response over the two year period was done, basal area increment and volume increment significantly responded to the application of nitrogen but not to phosphorus. Growth efficiency was not significantly influenced by either nitrogen or phosphorus over the full two year monitoring period. Water availability class consistently and significantly influenced basal area increment, volume increment and growth efficiency over the two year period as well as during year one and year two. The best responses generally occurred as a result of the additive effects of N and P. The growth response did not remain the same across the water availability classes. The wetter sites tended to have greater responses than the drier sites. Although these are still early results, the growth responses could be attributed to an increase in LAI. Nutrient analysis through vector analysis indicated that the additional N and P from fertilizer application were taken up by the trees thereby resulting in greater LAI and increased stem wood production.
AFRIKAANSE OPSOMMING: Die studie het ten doel gestel om die effek van mid-rotasie bemesting op blaar oppervlak indeks (BOI), basale oppervlakte- en volume aanwas te ondersoek in gedunde opstande van Pinus radiata op die mees algemene grondtipes van die Bolandstreek, Wes-Kaapland. Eksperimente is uitgelê oor 'n reeks van groeiplekke in die Bolandstreek wat gekies is om 'n water beskibaarheidsgradient te verteenwoordig oor elk van die twee mees algemene grondtipes. 'n Faktoriaal-kombinasie van kunsmisbehandelings met drie vlakke elk van stikstof [(N) teen 0, 100 en 200 kg ha-1] en fosfor [(P) teen 0, 50 en 100 kg ha-1] is toegedien. Hierdie ontwerp is vier maal herhaal oor 'n gradient van grondwater beskikbaarheid, oor elk van die twee mees algemene grondtipes, om sodoende 'n volledige eksperimentele reeks te vorm. Elke herhaling is uitgelê in 'n P. radiata opstand wat reeds 'n mid-rotasie dunning ondergaan het voor implementering van die kunsmis behandelings. Metings van BOI, deursnee op borshoogte, boomhoogte asook blaarmonsters is geneem voor implementering in 2008 en daarna met vooraf bepaalde tussenposes in 2009 en 2010. Die BOI en stam volume aanwas is bepaal om die effek van behandelings op groeieffektiwiteit te evalueer. Die gaping fraksie tegniek is gebruik om BOI te skat met behulp van 'n sonvlek septometer. Volume aanwas is bereken vanaf deursnee en hoogtemetings en basale oppervlak aanwas vanaf deursnee-metings. Metings van al bogenoemde groeireaksies is gebruik om die effek van verhoogde voedingstof beskikbaarheid op opstandsgroei te evalueer. Daar was geen betekenisvolle interaksies tussen enige van die faktore N, P of water beskikbaarheidsklas met betrekking tot reaksies op BOI, basale oppervlak- en volume aanwas of groei-effektiwiteit nie. Die BOI het betekenisvol gereageer op N en P in die eerste jaar, maar slegs op P in die tweede jaar na behandeling. Basale oppervlakte aanwas is betekenisvol verbeter deur N en P in die tweede jaar maar nie in die eerste jaar nie. Dit is waarskynlik as gevolg van die feit dat opstande eers hul kroondak moes herstel (na dunnings) voordat 'n reaksie in basale oppervlak verkry kon word. Vir die veranderlikes waar 'n analise van die groeireaksie oor die volle twee jaar moniteringsperiode gedoen is, het basale oppervlak- en volume aanwas betekenisvol gereageer op stikstof maar nie op fosfor nie. Groei-effektiwiteit is nie betekenisvol geaffekteer deur N of P oor die volle twee jaar moniteringsperiode nie. Water beskikbaarheidsklas het basale oppervlak en volume aanwas asook groei-effektiwiteit betekenisvol en voortdurend beïnvloed in die eerste en tweede jaar, asook gedurende die volle twee jaar moniteringsperiode. Die beste groeireaksie is oor die algemeen verkry waar N en P gesamentlik toegedien is en waar dus aanvullende reaksies verkry is. Groeireaksies het betekenisvol verskil na gelang van water beskikbaarheidsklas, met die grootste reaksie op die natste groeiplekke. Hoewel hierdie vroeë resultate is, kan ons die meganisme van die reaksie primêr toeskryf aan 'n toename in BOI. Vektor analise van blaar voedingstof vlakke het aangedui dat addisionele N en P na kunsmis toediening opgeneem is, wat die weg gebaan het vir 'n toename in BOI en verhoogde volume aanwas.

Interactions between plant roots and soil microorganisms in the rhizosphere are critical for plant growth. However, understanding of precisely how root exudates influence the diversity and activity of rhizosphere microorganisms is limited. The main objective of this study was to investigate the effect of radiata pine (Pinus radiata) root exudates on rhizosphere soil microbial communities, with an emphasis on the role of low molecular weight organic anions. The study involved the development and validation of new methods for investigating rhizosphere processes in a purpose-built facility. This included development of an in situ sampling technique using an anion exchange membrane strip to collect a range of organic anions exuded from radiata pine roots grown in large-scale rhizotrons. These included tartarate, quinate, formate, malate, malonate, shikimate, lactate, acetate, maleate, citrate, succinate and fumarate. Soil microbial activity and diversity were determined using dehydrogenase activity and denaturing gradient gel electrophoresis. Links between organic anions in root exudates and rhizosphere soil microbial community structures were investigated by comparing wild type and genetically modified radiata pine trees which were grown in rhizotrons for 10 months. As expected, there was considerable temporal and spatial variability in the amounts and composition of organic anions collected, and there were no consistent or significant differences determined between the two tree lines. Significant differences in rhizosphere microbial communities were detected between wild type and genetically modified pine trees; however, they were inconsistent throughout the experiment. The shifts in microbial communities could have been related to changes in exudate production and composition. Based on results from the main rhizotron experiment, a microcosm study was carried out to investigate the influence of selected pine root exudate sugars (glucose, sucrose and fructose) and organic anions (quinate, lactate and maleate) on soil microbial activity and diversity. Soil microbial activity increased up to 3-fold in all of the sugar and organic anion treatments compared to the control, except for a mixture of sugars and maleate where it decreased. The corresponding impacts on soil microbial diversity were assessed using denaturing gradient gel electrophoresis and 16S rRNA phylochips. Addition of the exudate compounds had a dramatic impact on the composition and diversity of the soil microbial community. A large number of bacterial taxa (88 to 1043) responded positively to the presence of exudate compounds, although some taxa (12 to 24) responded negatively. Organic anions had a greater impact on microbial communities than sugars, which indicated that they may have important roles in rhizosphere ecology of radiata pine. In addition, a diverse range of potentially beneficial bacterial taxa were detected in soil amended with organic anions, indicating specific regulation of rhizosphere microbial communities by root exudates. This project highlighted the considerable challenges and difficulties involved in detailed investigation of in situ rhizosphere processes. Nonetheless, the findings of this study represent a significant contribution to advancing understanding of relationships between root exudates and soil microbial diversity, which will be further enhanced by refinement and application of the specific methodologies and techniques developed.

Recent declines in returns from primary forest products in New Zealand and projected increases in world food prices have led to the land-use conversion from plantation forest to pastoral farming in many lowland areas. After decades of forest cover the soils are in many cases less than adequate for pastoral farming, as they are acidic, with toxic levels of exchangeable aluminum, and contain low levels of available nitrogen (N), very high carbon (C):N ratio, and are devoid of earthworms and structural integrity. Overcoming the major site limitations of low soil pH and available N was a major priority and a field experiment was established in April 2005 to determine the impact of various rates of lime and N in relation to pasture establishment and production. Concerns about the short and long-term effects of these inputs on biological soil quality gave rise to the present study. The effects of land-use change and establishment inputs were assessed by comparison of selected treatment plots with two adjacent reference sites (long-term pasture and a 60–year Pinus radiata forest) on the same soil type. The effects of lime and N on soil biological quality were investigated under field and controlled environment conditions by determination of: microbial community structure (phospholipid fatty acids - PLFA), microbial biomass (total PLFA), and microbial activity (dehydrogenase activity). Soil physical (percentage water-stable aggregates) and chemical (pH, and total C and N) properties were also determined. Similarly, the effects of earthworm addition on soil biological properties were explored in a short-term glasshouse pot experiment. The role of earthworms as indicators of soil biological quality in the field was assumed by nematodes and these were assessed in field trial plots and the reference sites mentioned above. Land-use change and applications of lime and N contributed to changing the microbial community structure determined by principal component analysis of transformed PLFA data. However, the effect of lime was more pronounced in the field, while N contributed most to changing microbial community structure in the glasshouse. Mean microbial activity in the field increased from 4 µg dwt/hr without lime to 16 and 21 µg dwt/hr where lime was applied at 5 and 10 tons/hectare (t/ha), respectively. Mean microbial activity in the field was markedly higher (7-fold) than in the glasshouse at similar rates of lime. Lime application also increased soil moisture retention in the field, mean gravimetric soil moisture increased from 0.33 in control plots to 0.38 and 0.39 in plots treated with 5 and 10 t/ha lime, respectively. Lime application was associated with greater soil aggregate stability. Soils from test plots treated with 5 and 10 tons/ha lime had 45-50% water-stable aggregates compared to 34% in treatments without lime. After 16 weeks in pots, earthworm treatments increased mean plant dry matter (DM)/pot by at least 19% above the control. The increase was attributed primarily to greater N mineralization in the presence of earthworms. For the duration of the trial the earthworm species tested (Apporectodea caliginosa and Lumbricus rubellus, individually or combined) did not affect any of the measured soil microbial properties. However, the survival rate of A. caliginosa was 83% compared to 25% for L. rubellus. The control not receiving any lime or N and plots treated with 10t/ha lime and 200 kgN/ha had similar nematodes species composition, comprising 40% each of bacterial and fungal feeding nematodes. They differed markedly from the reference sites as the forest soil was dominated by plant associated species (38%) and the long-term pasture had 44% plant parasitic nematodes. Accordingly, the soil food web condition inferred from nematode faunal analysis characterized all test plots as basal, stressed and depleted, while the forest soil was categorized as highly structured and fungal dominated. The findings of this thesis demonstrated that land-use change from forest to pasture can have significant impacts on soil biological properties, earthworms can contribute to pasture productivity even in the short term, and nematode faunal analysis is a robust and reliable indicator of soil biological quality.

Production of Pinus radiata is a major contributor to New Zealand's economy and new plantings are a valuable carbon sink. Phosphorus (P) deficiency and high P fixing capacity of some volcanic ash soils (e.g. Allophanic Soil) may constrain radiata productivity. This thesis investigates the role of ectomycorrhizal (ECM) root processes in the acquisition of P by P. radiata fiom native soil and soil fertilised with two reactive phosphate rock (RPR) fertilisers. The application of finely-divided RPRs to a P deficient Allophanic Soil significantly increased P. radiata seedling growth and P uptake in 10 month pot trials. RPR dissolution was high in this soil, and it was further enhanced by the radiata rhizosphere processes. The development and formation of ECM in radiata seedlings was stimulated by low rates of RPR application but was hindered in unfertilised soils and high rates of RPR application. The P. radiata ECM roots induced acidification and increased oxalate concentration and phosphatase activities in the rhizosphere soil. These changes in rhizosphere biochemical properties were associated with enhanced solubilisation of fertiliser and soil inorganic P and increased mineralisation of organic P, leading to increased P bioavailability in the rhizosphere. ECM inoculation of P. radiata roots with Rhizopogen rubescens and Suillus luteus stimulated production of phosphatase enzymes and oxalate and induced acidification in the rhizosphere. The extent of root-induced changes in the rhizosphere soils was associated with ECM hyphae length density. A technique using pulse labelling of radiata shoots with 14CO2 showed promise in estimating the active ECM hyphae density. The 14C activity was highly correlated with ECM hyphae density measured by an agar film technique. Overall, observations made in this thesis indicate that sparingly soluble forms of organic and inorganic P in soils low in plant-available P are readily solubilised and utilised for P. radiata growth through ECM rhizosphere processes.

The current silvicultural regimes of Pinus radiata plantations in New Zealand with wider initial tree spacings have created the potential for increased growth of understorey vegetation. A consequence of this is that the response of P. radiata to P fertiliser is expected to be more influenced by the interaction between the P fertiliser, the tree and the understorey vegetation than was the case in the past. The objectives of this study were to investigate the influence of different rates of a soluble and a sparingly-soluble P fertiliser (Triple superphosphate and Ben-Geurier phosphate rock) and weed control, and their interactions, on soil P chemistry and the growth and P uptake of 4-5-year-old second-rotation P. radiata on an Allophanic Soil (Kaweka forest) and a Pumice Soil (Kinleith forest). The results showed that the application of P fertilisers had no effect on P. radiata growth at both field trial sites two years after this treatment, although it increased radiata needle P concentration. However, at both sites, the understorey vegetation removal treatment increased tree diameter at breast height and basal area. At the highly P-deficient (Bray-2 P 4 µg g-1) Kaweka forest, the presence of understorey (bracken fern and some manuka) reduced resin-Pi and Olsen P concentrations, but at the moderate P fertility (Bray-2 P 13 µg g-1) Kinleith forest, the understorey (Himalayan honeysuckle, buddleia and some toetoe) increased Bray-2 P, resin-Pi, and Olsen P concentrations. A glasshouse study on P. radiata seedlings was conducted to test the hypothesis that when ryegrass (Lolium multiflorum) is grown with P. radiata, it increases radiata needle P concentration, while when broom (Cytisus scoparius L.) is grown with P. radiata, it has no effect. The acid phosphatase activity in the rhizosphere of P. radiata was higher when radiata was grown with broom than that when it was grown with ryegrass. This is consistent with the higher P concentration in needles of radiata grown with broom than that of radiata grown with ryegrass, in the absence of P fertiliser addition. However, when P fertiliser was added (50 and 100 µg P g-1 soil) the needle P concentration of radiata grown with broom was lower than that when radiata was grown with ryegrass.