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Zeeshan, Muhammad, Hafsa Zaneb, Saima Masood, Saima Ashraf, Imad Khan, Hafiz Faseeh Ur Rehman, Salahud Din i Khizar Hayat. "Morphological Modulation of Broiler Organs in Response to an Organic Acid–Phytogen Composite in Healthy Broilers". Agriculture 12, nr 6 (30.05.2022): 791. http://dx.doi.org/10.3390/agriculture12060791.
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In this study, we investigated broiler growth traits, intestinal health and immune aggregates in response to supplementation of Origanum vulgare and Cinnamomum verum, citric acid and malic acid in drinking water at varying levels and durations on broiler. We randomly allocated 140 day-old broilers into four (4) groups, with each group having seven replicates (n = 5). A basal diet and water were provided to all the groups ad libitum in accordance with standard farming practices. Keeping group I as the control, group II was supplemented with 1 mL/3.3 L of water for 1–4 days, 1 mL/4 L from 15–18 days and 1 mL/4 L water from 26–29 days. Group III and IV were supplemented with 1 mL/4 L of water and 1 mL/6 L of water for 35 days, respectively. After the completion of the trial (day 35), two broilers per replicate (14 birds per group) were slaughtered to sample the visceral organs. The results demonstrated that the feed conversion ratio (FCR) and body weight gain improved (p ≤ 0.05) during the fourth and fifth week of the trial in group III in comparison to the control group. Although the small intestine villus height and villus-height-to-crypt-depth ratio improved (p ≤ 0.05) in group III, the muscularis externa thickness of the ileum was highest (p ≤ 0.05) in the control group. Similarly, the lymphatic follicular and nodular area of the immune organs improved (p ≤ 0.05) in group III. In all segments of the small intestine (duodenal, jejunal and ileal), the number of acidic and total goblet cells was highest (p ≤ 0.05) in group II. In conclusion, dietary Origanum vulgare, Cinnamomum verum, citric acid and malic acid at the dose of 1 mL/4 L of drinking water (group III) was the most effective in improving the growth performance, gut development and associated immune components in broilers.
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Camargo, Karine Dalla Vecchia, Débora Siniscalchi, Natalia Vilas Boas Fonseca, Maria Luisa Curvelo Silva, Carlos Eduardo Vicente Junior, Déborah Marcia Felipe Rodrigues, Carlos Eduardo Lima i in. "PSXII-26 Effect of Different Supplements Plus Phytogenic Additives on Ruminal Bacterial Community of Grazing Beef Cattle". Journal of Animal Science 101, Supplement_3 (6.11.2023): 638–40. http://dx.doi.org/10.1093/jas/skad281.743.
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Abstract Changes in ruminal microbial community can affect animal productivity, nutrient utilization efficiency, and environmental impact. This study aimed to evaluate the effect of two levels of supplementation plus a blend of phytogenic additives (essential oils, saponins, and spices) on the ruminal bacterial community in beef cattle grazing tropical pastures. The experiment was conducted at the Beef Cattle Sector of UNESP, Jaboticabal-BR, and lasted 84 days. Nine castrated Nellore steers (body weight of 262 ± 31.2 kg) cannulated in the rumen were assigned to 3 simultaneous 3 × 3 Latin square designs. Animals were kept on pasture of Urochloa brizantha cv. Marandu during the rainy season. The treatments included ad libitum mineral supplementation (MS) as the control group, added mineral supplementation of 0.1% of body weight per day plus Cargill phytogenic blend (AMSP), and energetic-protein supplementation, 0.3% of body weight per day plus Cargill phytogenic blend (EPSP). Ruminal samples were collected on day 26 of each experimental period. A mix of liquid and solid rumen content (6 mL) was taken before supplementation and immediately frozen in liquid nitrogen. Total genomic DNA was extracted using the Quick-DNA Fecal/Soil Microbe kit. The V3-V4 hypervariable regions of the bacterial 16S rRNA gene were amplified using universal primers 341F and 806R. The PCR fragments were submitted to sequencing on an Illumina NovaSeq 6000 PE 250 platform. The sequences were processed using mothur, grouped into operational taxonomic units (OTUs) using a similarity cut-off value of 97%, and the bacterial OTUs were classified using the SILVA database. Bacterial community composition was compared between treatments using a Kruskal–Wallis test and Wilcoxon’s post-hoc test using Rstudio. A total of 27 phyla, 224 families, and 577 genera were assigned by the taxonomic analysis of the ruminal bacterial community (Figure 1). The phyla Bacteroidetes (44.45 ± 9.43) and Firmicutes (42.50 ± 7.18) represent over 86% of the total relative abundance in the rumen of Nellore steers and were not influenced by the supplementation (P ≥ 0.250). The relative abundances of the Spirochaetes phylum, Spirochaetaceae family, and Treponema genus decreased in the rumen of AMSP supplemented animals (P ≤ 0.037) and were similar between MS and EPSP (Figure 2). Additionally, the EPSP supplementation resulted in decreased relative abundances of TM7, TM7 family Incertae sedis, and TM7 genus Incertae sedis relative abundances compared with those in MS (P = 0.031) but not between AMSP and EPSP supplementations. The addition of phytotherapy blend in the supplements led to decreased SR1, SR1 family Incertae sedis, and SR1 genus Incertae sedis abundances in the rumen (P ≤ 0.012). This study indicates that the relative abundance of the dominant phyla was not affected by the supplementation levels, but the addition of the phytogenic blend in the supplement decreased the relative abundances of specific bacterial phyla, families, and genera,
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Olajumoke Temidayo Daramola i Patrick Acheneje. "Growth performance, serum metabolites, plasma protein fraction, meat lipid profile and antioxidant activities of breast meat of broiler chicken fed diets containing Cinnamomum ceylon powder, Zingiber officinale powder and Moringa oleifera leafmeal supplem". International Journal of Science and Research Archive 13, nr 1 (30.10.2024): 3141–52. http://dx.doi.org/10.30574/ijsra.2024.13.1.1716.
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An experiment was conducted to determine growth performance, serum metabolites, plasma protein fraction, meat lipid profile and antioxidant activities of meat of broilers as affected by Cinnamomium ceylon, Zingiber officinale and Moringa oleifera supplementation. A total of 112 day old chicks were allotted into four treatments replicated four times with seven birds per replicate in a completely randomized design (CRD). Basal diets were formulated for broiler starters (0-28days) and finisher phase (29-56) days. The basal diets were divided into 4 diets : diet 1- control diet without supplementation; diet 2- contained 0.2% Cinnamomum ceylon supplementation (CCS); diet 3- contained 0.2% Zingiber officinale supplementation (ZOS), diet 4-contained 0.2% Moringa oleifera leafmeal (MOLM) supplementation. The experiment lasted for 56 days. Results showed an improvement in final liveweight, weight gain, feed intake and feed conversion ratio of birds on 0.2% MOLM. Birds on phytogenic feed additives have lower values of urea, cholesterol, glucose, high density lipoprotein and low density lipoprotein. Birds administered phytogenic supplements exhibited much greater plasma albumin levels. Low levels of meat cholesterol, triglycerides and low density lipoprotein were found in birds fed phytogenic feed additives while high levels of high density lipoprotein were recorded for birds on phytogenic feed additives. The supplementation of broilers diets with phytogenic feed additives increased the glutathione peroxidase and superoxide dismutase and decreased lipid peroxidation. It was concluded that supplementation of broiler diets with Moringa oleifera leaf meal enhanced body weight gain, antioxidant activities also reduced cholesterol level in both serum metabolites and meat.
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May, Sabrina, Caroline Gonzalez Vega, Brooke Smith, Kory Moran i Ellyn Teddy. "190 Phytogenic feed additive improved growth performance and health of nursery pigs in diets with and without combination of several feed additives". Journal of Animal Science 102, Supplement_2 (1.05.2024): 138–39. http://dx.doi.org/10.1093/jas/skae102.154.
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Abstract An experiment was conducted to test the hypothesis that a phytogenic feed additive (Fresta Protect, Delacon, Austria) would improve growth performance and health indices of nursery pigs fed diets with or without other feed additives. Weaned pigs (n = 1,380; 19 d old with initial body weight (BW) = 5.5 ± 0.3 kg] were allotted to 10 replicate pens per treatment (23 pigs per pen) in 2 barns. Pens were blocked by location within barn and randomly allotted to one of 6 treatments: 1) Basal nursery feed without other feed additives (NC), 2) NC + 0.1% phytogenic (NC+P0.1), 3) Basal nursery feed with other feed additives such as organic acids, antioxidants, and blend of preservatives (PC), 4) PC + 0.05% phytogenic (PC+P0.05), 5) PC + 0.1% phytogenic (PC+P0.1), or 6) PC + 0.2% phytogenic (PC+P0.2). The experiment lasted for 39 d, with pigs fed experimental diets during phase 1 (d 0-10) and 2 (d 10-23) and a common diet without addition of PC or phytogenic during phase 3 (d 23-39). Pig and feed weights were recorded at the beginning and end of each phase. Performance data were analyzed using the lme4 package of R with treatment as fixed effect and barn and pen location within barn as random effects. Contrasts were used to test linear and quadratic effects of phytogenic in PC diets (diets 3 to 6), and diets 1, 2, 3, and 5 were used to compare main effects of phytogenic, diet type, and interaction between phytogenic and diet type. Results indicated that pigs fed diets with phytogenic, regardless of diet type, had increased (P < 0.05) average daily gain (ADG) and gain to feed ratio (G:F) in phase 1, final BW, ADG, and average daily feed intake (ADFI) in phase 2, as well as overall ADG and ADFI. The changes observed in final BW and ADFI in phase 2 had a quadratic response (P < 0.05) as dietary inclusion of phytogenic increased, whereas overall ADG linearly increased (P < 0.05) with greater levels of phytogenic supplementation (Table 1). In phase 2, the phytogenic supplementation increased G:F to a greater extent when diets did not contain other feed additives compared with PC diets (phytogenic x feed type interaction: P < 0.05). Phytogenic supplementation numerically reduced mortality. Pigs fed PC diets, regardless of inclusion of phytogenic, had increased (P < 0.05) final BW, ADG, and G:F in phase 1, final BW, ADG, and ADFI in phase 2, and overall ADG and ADFI compared with pigs fed NC diets. In conclusion, phytogenic supplementation with an optimal dietary inclusion of 0.1% improved growth performance, regardless of supplementation of other feed additives in the diet, and diets containing additional feed additives further improved performance of pigs.
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Daud, M., M. A. Yaman i Zulfan. "Effects of rations containing leubiem fish (Chanthidermis maculatus) waste and phytogenic suplementation on local ducks performance in the starter phase". IOP Conference Series: Earth and Environmental Science 922, nr 1 (1.11.2021): 012041. http://dx.doi.org/10.1088/1755-1315/922/1/012041.
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Abstract This study aims to examine the potential use of leubiem fish (Chanthidermis maculatus) waste and phytogenic supplementation on ration, in order to increase the performance of local ducks in the starter phase. This study used 100 local ducks aged 1 week old, and maintained them until 8 weeks old. The ducks were placed in 5 treatments and 4 replications groups (5 ducks / repetition). The study was conducted experimentally, using a Completely Randomized Design (CRD), consisting of 5 ration treatments, including: R1 (control); R2 (basal ration containing leubiem fish skin flour 10% + phytogenic 0.5%); R3 (basal ration containing leubiem fish head flour 10% + phytogenic 0.5%); R4 (basal ration containing leubiem fish bone flour 10% + phytogenic 0.5%) and R5 (basal ration containing leubiem fish gill flour + phytogenic 0.5%). Observed variables were feed consumption, body weight gain, final weight, feed conversion, and local duck mortality in the starter phase. The data obtained were both analyzed using Analysis of Variance (ANOVA) and Duncan’s multiple range test. The results showed that the use of leubiem fish waste and phytogenic supplementation in the ration both increased the performance of local ducks in the starter phase and had a significant effect (p <0.05) on ration consumption, weight gain, and final weight. However, they had no significant effect on feed conversion and local duck mortality in the starter phase. It was concluded that the use of leubiem fish (10% bone meal) and phytogenic supplementation 0.5% in the ration was able to increase body weight gain, and the final weight of the starter phase local ducks (aged 1-8 weeks).
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Siniscalchi, Débora, Karine Dalla Vecchia Camargo, Natalia Vilas Boas Fonseca, Marcelo Augusto Oliveira Castro, Isadora Alves Dornellas, Willian Aparecido De Souza, Lucas Dos Santos Del Vecchio i in. "PSX-13 Effects of Different Supplementations During the Rainy Season on Nitrogen Metabolism of Beef Cattle Steers on Tropical Pasture". Journal of Animal Science 101, Supplement_3 (6.11.2023): 508–9. http://dx.doi.org/10.1093/jas/skad281.600.
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Abstract Phytogenic additives are used as food additives in ruminant nutrition due to their potential to alter the nitrogen (N) excretion route and minimize N emissions into the environment. This study aimed to evaluate the effect of two levels of supplementation plus a mixture of phytogenic additives (essential oils, saponins and spices) on the efficiency of N utilization by beef cattle in tropical pastures. The experiment was conducted at the Beef Cattle Sector of UNESP, Jaboticabal-BR, and lasted 84 days. Nine castrated Nelore steers (body weight 262 ± 31.2 kg) cannulated in the rumen were distributed in 3 simultaneous 3 × 3 Latin square designs. The animals were kept in pasture of Urochloa brizantha cv. Marandu during the rainy season. Treatments included ad libitum mineral supplementation (MS) as a control group, mineral supplementation added 0.1% of body weight per day plus Cargill phytogenic mixture (AMSP) and energy-protein supplementation, 0.3% of body weight per day plus Cargill phytogenic mixture (EPSP). Point urine and fecal samples were collected in the last three days of each experimental period. The concentration of total nitrogen in the urine was analyzed by the Kjeldahl procedure. The nitrogen composition of forage, supplements and feces was estimated by near-infrared spectroscopy (NIRS) using a modular FT-NIR spectrometer (NIRFlex N500, Büchi Labortechnik AG, Flawil, Switzerland). Nitrogen retention was calculated using the following equation: NR = N intake (g/d) – [N excretion in feces (g/d) + N excretion in urine (g/d)]. The nitrogen metabolism data were analyzed as a 3 × 3 Latin square with 3 replications, balanced for residual effects. Considering as fixed effect the treatment, and as random effect the Latin square, period, steer, steer × period interaction, and residues corresponding to the model. The data obtained were submitted to ANOVA and Tukey's test (P ≤ 0.05), using the statistical program R, version 3.4.4. The addition of phytogenic additives in the supplements led to decreased (P < 0.036) urinary excretion of N (g/day) from 41.16 g/day to 28.38 g/day and urinary excretion of N (% of N intake) from 30.66% to 23.21%, but did not differ between the different levels of phytogenic supplementation (Table 1). In addition, retained N (% of N intake) was greater (P < 0.01) in animals supplemented with AMSP and EPSP compared with MS, increasing N retention from 26.78% to 33.88%. This study indicates that the different levels of supplementation plus the addition of the phytogenic mixture improved the efficiency of N utilization by decreasing the excretion of N in the urine and increasing the retained N compared with MS supplementation.
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Althaber, Colton A., Ken Coffey, James D. Caldwell i Michael C. Pruden. "170 Effects of Dietary Phytogenic Supplementation to a Finishing Diet on Intake, Digestibility, Nitrogen Balance and Carcass Measures". Journal of Animal Science 98, Supplement_4 (3.11.2020): 125. http://dx.doi.org/10.1093/jas/skaa278.228.
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Abstract Dietary manipulations to include tannins can change the proportion and amount of nitrogen (N) excreted in the urine and feces as well as improve N-use efficiency in ruminants. Our objective was to determine the effects of different levels of phytogenic supplementation (Actifor®) on intake, digestibility, carcass measures, and N balance of lambs offered a corn-based finishing diet. Twenty-six lambs (31.5 ± 5.39 kg BW) were allocated randomly to 1 of 3 corn-based finishing diets (85% concentrate) that were offered for ad libitum consumption. Lambs were housed individually in 1 × 1.5-m pens with plastic-coated grate flooring (n = 18; 6/trt) or 1.5 × 4.3-m pens with rubber mat flooring (n = 8; 2 or 3/trt). Diets were not supplemented or supplemented with 4 or 8 g/d of Actifor® (n = 8, 9, and 9 lambs/trt, respectively). A 7-d total fecal and urine collection period (n = 6 lambs/trt) began on d 43. On d 50, rumen samples were taken at 0800, 1100, and 1400 h for measurement of pH and VFA concentrations. Data were analyzed using the MIXED procedure of SAS, and orthogonal linear and quadratic contrasts were used to assess phytogenic supplementation level effects. Intake and digestibility of DM and OM were not different across treatments (P ≥ 0.32). Average fecal N and lamb shrink before slaughter increased linearly (P ≤ 0.05) with increasing levels of phytogenic supplementation. Propionate and acetate:propionate decreased (P ≤ 0.05) and acetate, valerate, and pH tended to increase quadratically (P ≤ 0.08) with increasing phytogenic inclusion. Back-fat and yield grade tended (P = 0.06) to decrease linearly and dressing percentage tended (P = 0.06) to decrease quadratically with increasing phytogenic inclusion. Phytogenic supplementation increased average fecal N and improved some carcass measures, but did not affect diet digestibility.
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Morón-Ortiz, Ángeles, Antonis A. Karamalegkos, Paula Mapelli-Brahm, Marina Ezcurra i Antonio J. Meléndez-Martínez. "Phytoene and Phytoene-Rich Microalgae Extracts Extend Lifespan in C. elegans and Protect against Amyloid-β Toxicity in an Alzheimer’s Disease Model". Antioxidants 13, nr 8 (31.07.2024): 931. http://dx.doi.org/10.3390/antiox13080931.
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Phytoene is a colourless carotenoid widely available from dietary sources and a precursor for the synthesis of other carotenoids. Although present at high concentrations across different tissues, phytoene is largely viewed as not having physiological activity. Here, we utilize the model organism C. elegans to show that phytoene is bioactive and has anti-ageing properties. Supplementation with phytoene protects against oxidative damage and amyloid-β42 proteotoxicity (a major pathology of Alzheimer’s disease), and extends lifespan. We also examine extracts from two microalgae, Chlorella sorokiniana and Dunaliella bardawil. We show that the extracts contain high levels of phytoene, and find that these phytoene-rich extracts have protective effects similar to pure phytoene. Our findings show that phytoene is a bioactive molecule with positive effects on ageing and longevity. Our work also suggests that phytoene-rich microalgae extracts can utilized to produce foods or supplements that promote healthy ageing and prevent the development of chronic age-related diseases.
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Camargo, Karine Dalla Vecchia, Débora Siniscalchi, Natalia Vilas Boas Fonseca, Willian Aparecido De Souza, Marcelo Augusto Oliveira Castro, Barbara Carolina Afonso, Isadora Alves Dornellas i in. "PSXI-30 Ruminal Bacterial Diversity Indexes of Grazing Beef Cattle Receiving Different Supplements Plus Phytogenic Additives". Journal of Animal Science 101, Supplement_3 (6.11.2023): 634–36. http://dx.doi.org/10.1093/jas/skad281.739.
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Abstract The addition of phytogenic compounds that have antimicrobial activity in cattle supplements can promote changes in ruminal bacterial diversity. This study aimed to evaluate the effect of two levels of supplementation plus a blend of phytogenic additives (essential oils, saponins, and spices) on the measures of alpha and beta diversity in the rumen of beef cattle grazing tropical pasture. The experiment was carried out in the Beef Cattle Sector of UNESP, Jaboticabal-BR. The trial lasted 84 days and nine castrated Nellore steers (body weight of 262 ± 31.2 kg) cannulated in the rumen were assigned in 3 simultaneous 3 × 3 Latin square designs. Animals were kept on the pasture of Urochloa brizantha cv. Marandu in the rainy season. The treatments were: ad libitum mineral supplementation (MS) as control group; added mineral supplementation, 0.1% of body weight per day plus Cargill phytogenic blend (AMSP); and energetic-protein supplementation, 0.3% of body weight per day plus Cargill phytogenic blend (EPSP). Ruminal samples were collected on day 26 of each experimental period. A mix of liquid and solid rumen content (6 mL) was taken before supplementation, placed in cryogenic tubes, and immediately frozen in liquid nitrogen. All samples were stored at −80 °C until DNA extraction and analysis. Total genomic DNA was extracted using the Quick-DNA Fecal/Soil Microbe Miniprep kit. The V3-V4 hypervariable regions of the bacterial 16S rRNA gene were amplified primers 341F (CCTACGGGNGGCWGCAG) and 806R (GGACTACHVGGGTWTCTAAT). The PCR fragments were submitted to sequencing on an Illumina NovaSeq 6000 PE 250 platform. The sequences were processed using mothur, grouped into operational taxonomic units (OTUs) using a similarity cut-off value of 97%, and the bacterial OTUs were classified using the SILVA database. Alpha diversity indices including Shannon, Simpson, Fisher, Chao1, and ACE, were calculated using the phyloseq package, while beta diversity was determined using the vegan package, both in Rstudio. Alpha diversity indices were compared between treatments using a non-parametric Kruskal–Wallis test and Wilcoxon’s post-hoc test. Beta diversity was tested using permutational multivariate analysis of variance (PERMANOVA) based on the Bray-Curtis similarity index. The AMSP and EPSP did not affect measures of alpha diversity (Figure 1), such as Shannon and Fisher indices (P = 0.219) as well as the richness index measured using Chao1 and ACE (P ≥ 0.363). A negative effect of supplementation on alpha diversity (P = 0.004) was only observed with Simpson’s Index of Diversity. Additionally, the supplementation did not change the beta-diversity of the ruminal bacterial community based on Bray–Curtis similarity index (P = 0.510; Figure 2). In conclusion, the addition of phytogenic compounds in the two levels of supplementation showed only a minor impact on the alpha diversity in the rumen of grazing beef cattle.
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Ren, Xipeng, Maria M. Whitton, Sung J. Yu, Tieneke Trotter, Yadav S. Bajagai i Dragana Stanley. "Application of Phytogenic Liquid Supplementation in Soil Microbiome Restoration in Queensland Pasture Dieback". Microorganisms 11, nr 3 (23.02.2023): 561. http://dx.doi.org/10.3390/microorganisms11030561.
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Pasture production is vital in cattle farming as it provides animals with food and nutrients. Australia, as a significant global beef producer, has been experiencing pasture dieback, a syndrome of deteriorating grassland that results in the loss of grass and the expansion of weeds. Despite two decades of research and many remediation attempts, there has yet to be a breakthrough in understanding the causes or mechanisms involved. Suggested causes of this phenomenon include soil and plant microbial pathogens, insect infestation, extreme heat stress, radiation, and others. Plants produce a range of phytomolecules with antifungal, antibacterial, antiviral, growth-promoting, and immunostimulant effects to protect themselves from a range of environmental stresses. These products are currently used more in human and veterinary health than in agronomy. In this study, we applied a phytogenic product containing citric acid, carvacrol, and cinnamaldehyde, to investigate its ability to alleviate pasture dieback. The phytogenic liquid-based solution was sprayed twice, one week apart, at 5.4 L per hectare. The soil microbial community was investigated longitudinally to determine long-term effects, and pasture productivity and plant morphometric improvements were explored. The phytogenic liquid significantly improved post-drought recovery of alpha diversity and altered temporal and spatial change in the community. The phytogenic liquid reduced biomarker genera associated with poor and polluted soils and significantly promoted plant and soil beneficial bacteria associated with plant rhizosphere and a range of soil benefits. Phytogenic liquid application produced plant morphology improvements and a consistent enhancement of pasture productivity extending beyond 18 months post-application. Our data show that phytogenic products used in the livestock market as an alternative to antibiotics may also have a beneficial role in agriculture, especially in the light of climate change-related soil maintenance and remediation.
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May, Sabrina, Caroline Gonzalez Vega i Molly L. McGhee. "234 Effects of Feeding a Phytogenic Blend with or without Supplementation of Specialty Fats on Growth Performance of Nursery Pigs". Journal of Animal Science 101, Supplement_2 (28.10.2023): 141–42. http://dx.doi.org/10.1093/jas/skad341.159.
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Abstract An experiment was conducted to test the hypothesis that inclusion of a phytogenic feed additive (Fresta Protect, Delacon, Linz, Austria), separately or in conjunction with specialty fats (modified soy oil blend and medium chain fatty acid blend), in diets for nursery pigs would improve growth performance. Weaned pigs [n = 4,400; 5.7 ± 0.1 kg body weight (BW)] were allotted to 50 replicate pens per treatment (20 to 23 pigs per pen) in 5 barns. Pens were blocked by location within barn and randomly allotted to 4 treatments in a 2 x 2 factorial arrangement. Treatment main effects were specialty fats inclusion in phase 1 (0 or 1.3%) and phytogenic inclusion in phase 1 and 2 (0 or 0.1%). Pigs were fed experimental diets during phase 1 and 2 (day 0 – 10 and 10 – 21, respectively), and pig and feed weights were recorded at the beginning and end of each phase. Data were analyzed using the lme4 package of R 4.1.2, and the statistical model included the fixed effects of specialty fats, phytogenic, and their interaction, as well as the random effects of barn and location within barn. In phase 1, an interaction was detected (P < 0.05) for final BW, average daily gain (ADG), average daily feed intake (ADFI), and gain:feed (G:F; Table 1). In general, phase 1 performance was improved by phytogenic inclusion when no specialty fats were present in the diet, but pigs did not benefit from inclusion of the phytogenic when fed in combination with specialty fats. Body weight at the end of phase 2 was reduced (P < 0.05) for pigs fed specialty fats, but inclusion of the phytogenic increased (P < 0.05) phase 2 final BW and ADG regardless of specialty fat inclusion. Supplementation of the phytogenic increased (P < 0.05) phase 2 G:F as well, and the improvement in G:F was more pronounced when fed in conjunction with specialty fats, though this observation may be partially confounded by compensatory gain for the specialty fats and phytogenic combination treatment. The phytogenic also increased (P < 0.05) overall ADG and G:F, whereas the main effect of specialty fats reduced (P < 0.05) overall ADG and G:F. In conclusion, dietary inclusion of a phytogenic feed additive improved growth performance of nursery pigs, whereas specialty fats decreased growth performance, and the best performance was achieved when the phytogenic was fed in the absence of specialty fats.
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Koseli, E., N. Seyidoglu, R. Gurbanli i C. Aydin. "The influence of phytogenic additive on the antioxidant capacity, immunity and liver functions in stress-induced male rats". Journal of the Hellenic Veterinary Medical Society 73, nr 3 (9.11.2022): 4449–58. http://dx.doi.org/10.12681/jhvms.27432.
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Global animal feeding strategies have been modified due to several ecosystem changes that cause stress and decline in health, growth, and yields. According to the literature, natural additives have been fundamental to animal health from day to day. The present study measured changes in antioxidant status, immunity, liver functions and organ weights tested under stress conditions to determine whether dietary supplementation with phytogenic additive could provide beneficial effects. Forty-eight adult, male Sprague-Dawley rats were randomly separated into four groups; Control(C), Stress(S), Treatment(Tr), Treatment and Stress(TrS). Rats in groups Tr and TrS received phytogenic additive by adding into water (2mL/L) 5days a week for 28days. All rats were exposed to prolonged light phase conditions (18h light: 6h dark) for 14 days. Also, two chronic stresses, isolation and crowded environments, were applied to animals in the Stress and TrS groups. There was a significant decline in the oxidant status in untreated stress group, while phytogenic additive fed rats maintained a significantly higher total antioxidant status. This study also showed a significant increase in IL-4 and decrease in IFN-γ in the untreated Stress group compared to the Control group. There were increases in liver enzymes in the Stress group in comparison to the Control group. After the phytogenic treatment, there was an increase in the weight of the liver, intestine, brain and testes. In conclusion, this study showed that supplementation of phytogenic additive containing milk thistle and artichoke with choline, carnitine, vitamin E and melatonin describes the protective effects on antioxidant status, immunological parameters and liver functions under mixed stress conditions.
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Olarotimi, Olumuyiwa Joseph. "Turmeric (Curcuma Longa): An Underutilized Phytogenic Additive in Poultry Nutrition". Turkish Journal of Agriculture - Food Science and Technology 6, nr 1 (4.02.2018): 102. http://dx.doi.org/10.24925/turjaf.v6i1.102-106.1572.
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The aim of this review paper is to explore the available information from research findings on the use of Turmeric (Curcuma longa) as a reliable phytogenic antibiotic for poultry production in the tropics especially in Nigeria and other countries. A wide range of phytogenic additives has found a growing interest in fortification of poultry diets. Supplementation of natural alternatives to antibiotic growth promoters in order to maintain both birds’ performance and health in the tropics will be a welcome development. Turmeric has reportedly been widely supplemented in poultry diets as natural alternative antibiotics in several studies with outstanding performances. The nutritional, medicinal, haematological and phyto-toxicological effects of turmeric were reviewed in this paper. Hence, turmeric supplementation at recommended inclusion rate in poultry feeds without posing any deleterious effects to the birds’ performances as well as the consumers of the products, and ultimately, leading to better profitability on the part of the farmers, will be a nutritional breakthrough for the farmers in the tropics.
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Song, Jun Ho, Md Raihanul Hoque, Md Mortuza Hossain i Inho Kim. "PSVI-11 Flavonoid Supplementation to Low Protein Diets Recovers Growth Performance in Broilers". Journal of Animal Science 100, Supplement_2 (12.04.2022): 163–64. http://dx.doi.org/10.1093/jas/skac064.279.
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Abstract Recently, phytogenic feed additives containing flavonoids, essential oils and phenols are getting more attention as they have antimicrobial, anti-inflammatory, and digestibility aiding abilities. Flavonoids have proved their abilities in reducing environmental stress along with activating immune responses. These positive impacts can be used to bring an improved growth performance in broilers, so we aimed to evaluate the effect of flavonoid supplementation to a low protein diet on growth performance, nutrient digestibility, excreta bacterial count and excreta gas emission in broilers. In total 800 one-day-old Ross 308 broilers (average body weight of 42.90±1.43 g) were divided randomly into 1 of 4 dietary treatments. Each treatment had 10 replicate pens with 20 chickens. Treatment diets were HCP (High protein diet), basal diet; LCP (Low protein diet), basal diet – 2.5% protein; TRT1, LCP + 0.025 flavonoid; TRT2, LCP + 0.050% flavonoid. Experimental diets were provided to broilers from d 8 to 35. HCP, LCP supplemented with flavonoid showed greater (P < 0.05) body weight gain (BWG) than the low protein diet (LCP) without flavonoid supplementation during d 8 to 21, and in the overall experiment. However, feed conversion ratio (FCR) of HCP group was reduced (P < 0.05) than the LCP group during d 8 to 21 and in overall period. Increasing level of flavonoid supplementations brought a linear increase in BWG during d 8 to 21 (P = 0.0141), and overall period (P = 0.0153). Increasing doses of flavonoid showed a tendency of gradual increase (P = 0.0702) in dry matter (DM) digestibility in broilers. Lactobacillus, E. coli and Salmonella counts in excreta samples of experimental groups showed no significant difference (P > 0.05). Flavonoid supplementation (0.050%) in low protein diet reduced (P < 0.05) drip loss in breast muscle than the low protein diet with 0% flavonoid supplementation. In short, flavonoid supplementation to a low protein diet recovered the body weight gain through better digestion broilers
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Jócsák, Ildikó, János Tossenberger, György Végvári, Gergő Sudár, Éva Varga-Visi i Tamás Tóth. "How Is the Effect of Phytogenic Feed Supplementation Tested in Heat Stressed Pigs? Methodological and Sampling Considerations". Agriculture 10, nr 7 (2.07.2020): 257. http://dx.doi.org/10.3390/agriculture10070257.
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Climate change will lead to increasingly hot summers where the temperature rises above the thermoneutral range of pigs; as a result, they get exposed to heat stress. One of the most damaging consequences of long-lasting heat stress is oxidative stress arising from the increasing level of reactive oxygen species. In order to eliminate oxidative stress, metabolites that are needed for maintaining life and growth may get depleted, which, in chronic cases in particular, negatively affects the economy of meat production. The effect of plant-originated phytogenic feed additives with high antioxidant content may be beneficial to pigs in reducing the effects of oxidative stress induced by heat stress. In this study, a range of methods that assess the effects of phytogenic feed additives on heat stress are reviewed. The main focus is presenting an overview of the investigational possibilities of the antioxidative system and feed uptake and utilization via traditional methods and molecular biological investigations. Furthermore, methodological aspects of sampling are taken into consideration in order to select the best methods for determining the effect of phytogenic feed supplementation on heat-stressed pigs.
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Abdelli, Nedra, David Solà-Oriol i José Francisco Pérez. "Phytogenic Feed Additives in Poultry: Achievements, Prospective and Challenges". Animals 11, nr 12 (6.12.2021): 3471. http://dx.doi.org/10.3390/ani11123471.
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Phytogenic feed additives have been largely tested in poultry production with the aim to identify their effects on the gastrointestinal function and health, and their implications on the birds’ systemic health and welfare, the production efficiency of flocks, food safety, and environmental impact. These feed additives originating from plants, and consisting of herbs, spices, fruit, and other plant parts, include many different bioactive ingredients. Reviewing published documents about the supplementation of phytogenic feed additives reveals contradictory results regarding their effectiveness in poultry production. This indicates that more effort is still needed to determine the appropriate inclusion levels and fully elucidate their mode of actions. In this frame, this review aimed to sum up the current trends in the use of phytogenic feed additives in poultry with a special focus on their interaction with gut ecosystem, gut function, in vivo oxidative status and immune system as well as other feed additives, especially organic acids.
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El-Ashram, Saeed, i Gamal A. Abdelhafez. "Effects of phytogenic supplementation on productive performance of broiler chickens". Journal of Applied Poultry Research 29, nr 4 (grudzień 2020): 852–62. http://dx.doi.org/10.1016/j.japr.2020.07.005.
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Yu, Sung J., Yadav S. Bajagai, Friedrich Petranyi, Sara de las Heras-Saldana, Thi Thu Hao Van i Dragana Stanley. "Phytogenic Blend Improves Intestinal Health and Reduces Obesity, Diabetes, Cholesterol and Cancers: A Path toward Customised Supplementation". Antibiotics 11, nr 10 (18.10.2022): 1428. http://dx.doi.org/10.3390/antibiotics11101428.
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Poultry production is among the most challenging industries for pathogen control. High animal density and abundance of faecal material demand strict biosecurity measures and continual vigilance in monitoring animal health parameters. Despite this vigilance, dealing with disease outbreaks is a part of farmers’ routines. Phytogenic feed additives comprised of herbs, spices, essential oils, and oleoresins have potent antimicrobial and anti-inflammatory actions. Related studies are gaining substantial interest in human and animal health worldwide. In this study, a commercial blend phytogenic feed additive was supplemented to layers in an industrial free-range production system with 20,000 birds in both control and treatment groups. At the end of the trial, the ileum tissue was sampled for RNAseq transcriptomic analysis to study the host reaction to the supplement. Phytogenic supplement significantly inhibited four cholesterol-related pathways and reduced the Arteriosclerosis disease category towards improved cardiovascular health. The supplemented birds exhibited reduced disease susceptibility for 26 cancer categories with p-values in the range from 5.23 × 10−4 to 1.02 × 10−25. Major metabolic shifts in Lipid metabolism in combination with Carbohydrate metabolism have resulted in a decrease in the Obesity category, altering the ratio of fat and carbohydrate metabolism toward lower fat storage.
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Santos, Gleyson Araújo dos, Maria do Carmo de Oliveira, Amanda Medeiros Araújo de Oliveira, Victor Hugo Teixeira Batista, Priscila Oliveira Costa, Augusto Heck, Camilla Mendonça Silva, Adriano Henrique do Nascimento Rangel, Michelly Fernandes de Macedo i Rennan Herculano Rufino Moreira. "Phytogenic Bioactive Compounds in the Diet of Lactating Sows, Litter Performance, and Milk Characteristics". Animals 13, nr 17 (30.08.2023): 2764. http://dx.doi.org/10.3390/ani13172764.
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The objective was to evaluate the effect of phytogenic supplementation in the feed of lactating sows on the performance parameters of sows and suckling piglets. Ninety-three lactating sows of commercial lines (sows TN70) were distributed in a completely randomized design. The treatments adopted were a control diet without phytogenics (control) and a control diet with phytogenic supplementation in the feed. Performance parameters and the behaviors of sows and piglets were evaluated, in addition to milk composition and the biochemical parameters of the animals. The use of phytogenics did not affect the feed intake or tissue mobilization of the sows. However, it improved the production and quality of milk and reduced the possibility of light piglets at weaning by 22.16 percentage points. Regarding biochemical parameters, phytogenics improved animal recovery in the lactation period, as proven by an increase in the serum concentration of total protein and urea. Sows that received phytogenics exhibited increased behaviors of inactivity (3.16%), breastfeeding (1.15%), and water consumption (0.95%). In conclusion, using phytogenics in the feed of lactating sows improves the performance of the litters without affecting the body condition of the sows, with increased milk production and enhanced protein and lactose concentration.
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Ismoyowati, Ismoyowati, Diana Indrasanti, Sigit Mugiyono i Mulyoto Pangestu. "Phytogenic compounds do not interfere physiological parameters and growth performances on two Indonesian local breeds of ducks". November-2019 12, nr 11 (listopad 2019): 1689–97. http://dx.doi.org/10.14202/vetworld.2019.1689-1697.
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Aim: The present study was to investigate the interaction between duck's breed and phytogenic compounds as feed additives in the diet on blood lipid and hematological profile, welfare, and growth performance. Materials and Methods: A total of 200 male day-old local breed ducks (Tegal and Muscovy ducks) were used in this experiment. The first factor was duck breed and the second factor was different phytogenic compounds supplementation in the diet: Garlic, turmeric, ginger, and kencur, at 3% each. The observed variables were the blood lipid profiles comprise high-density lipoprotein (HDL), low-density lipoprotein, cholesterol total, triglyceride, blood parameters, welfare (heterophil/ lymphocyte [H/L] ratio), and growth performances (feed consumption, body weight gain, feed conversion ratio, and carcass percentage). Results: The interaction between breed of ducks and phytogenic compounds had a significant effect on blood triglyceride, but no significant effect on the blood lipid profile, hematological parameters, and growth performances. While, phytogenic compounds in the diet had significant effects on the blood lipid profile, heterophil (H), lymphocyte (L), and H/L ratio of ducks. The breed factors affected HDL and growth performances. Muscovy duck had a higher HDL and growth performance compare to Tegal duck. Among those, garlic most effectively reduced triglyceride level in Tegal duck. Conclusion: Phytogenic compounds 3% do not have a negative effect on the physiological parameters of ducks increase ducks welfare (H/L ratio), so it does not affect the growth performances of ducks. Muscovy duck had higher growth performances than Tegal ducks.
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Mahmmod, Zhian A., Hurea Saber Abdulrazaq i Nawal Kamal Shokri. "Influence of supplementation three type of Phytogenic plants in diet on growth Performance, intestinal microflora and immunity of Broiler Cobb-500." Journal of Zankoy Sulaimani - Part A 19, nr 2 (20.11.2016): 4352–42. http://dx.doi.org/10.17656/jzs.10610.
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Popov, Igor V., Naiana Einhardt Manzke, Mônica Maurer Sost, Jessica Verhoeven, Sanne Verbruggen, Iuliia P. Chebotareva, Alexey M. Ermakov i Koen Venema. "Modulation of Swine Gut Microbiota by Phytogenic Blends and High Concentrations of Casein in a Validated Swine Large Intestinal In Vitro Model". Veterinary Sciences 10, nr 12 (27.11.2023): 677. http://dx.doi.org/10.3390/vetsci10120677.
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Phytogenic feed additives are gaining popularity in livestock as a replacement for antibiotic growth promotors. Some phytogenic blends (PB) positively affect the production performance, inhibit pathogens within the gut microbiota, and improve the overall health of farm animals. In this study, a swine large intestine in vitro model was used to evaluate the effect of two PBs, alone or in combination with casein, on swine gut microbiota. As a result, the combination of casein with PB1 had the most beneficial effects on swine gut microbiota, as it increased the relative abundance of some commensal bacteria and two genera (Lactobacillus and Oscillospiraceae UCG-002), which are associated with greater production performance in pigs. At the same time, supplementation with PBs did not lead to an increase in opportunistic pathogens, indicating their safety for pigs. Both PBs showed fewer changes in swine gut microbiota compared to interventions with added casein. In contrast, casein supplementation significantly increased beta diversity and the relative abundance of commensal as well as potentially beneficial bacteria. In conclusion, the combination of casein with PBs, in particular PB1, had the most beneficial effects among the studied supplements in vitro, with respect to microbiota modulation and metabolite production, although this data should be proven in further in vivo studies.
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Weng, Huan-Xin, Hui-Ping Liu, De-Wang Li, Mingli Ye, Lehua Pan i Tian-Hong Xia. "An innovative approach for iodine supplementation using iodine-rich phytogenic food". Environmental Geochemistry and Health 36, nr 4 (7.02.2014): 815–28. http://dx.doi.org/10.1007/s10653-014-9597-4.
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Prachumchai, Rittikeard, Chaichana Suriyapha, Gamonmas Dagaew, Sukruthai Sommai, Maharach Matra, Srisan Phupaboon, Yupin Phasuk i Metha Wanapat. "Microencapsulation of lemongrass and mangosteen peel as phytogenic compounds to gas kinetics, fermentation, degradability, methane production, and microbial population using in vitro gas technique". PLOS ONE 19, nr 6 (5.06.2024): e0304282. http://dx.doi.org/10.1371/journal.pone.0304282.
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The purpose of the current study was to evaluate the impact of various doses of microencapsulated lemongrass and mangosteen peel (MELM) on gas dynamics, rumen fermentation, degradability, methane production, and microbial population in in vitro gas experiments. With five levels of microencapsulated-phytonutrient supplementation at 0, 1, 2, 3, and 4% of substrate, 0.5 g of roughage, and a concentrate ratio of 60:40, the trial was set up as a completely randomized design. Under investigation, the amount of final asymptotic gas volume was corresponding responded to completely digested substrate (b) increased cubically as a result of the addition of MELM (P < 0.01) and a cubic rise in cumulative gas output. The amount of MELM form did not change the pH and NH3-N concentration of the rumen after 12 and 24 h of incubation. However, methane production during 24 h of incubation, the levels were cubically decreased with further doses of MELM (P < 0.01) at 12 h of incubation. Increasing the dosage of MELM supplementation at 2% DM resulted in a significant increase in the digestibility of in vitro neutral detergent fiber (IVNDF) and in vitro true digestibility (IVTD) at various incubation times (P < 0.05), but decreased above 3% DM supplementations. Moreover, the concentration of propionic acid (C3) exhibited the variations across the different levels of MELM (P < 0.05), with the maximum concentration obtained at 2% DM. The populations of Fibrobacter succinogenes, Ruminococcus albus, Ruminococcus flavefaciens, and Megasphaera elsdenii revealed a significant increase (P < 0.05), while the quantity of Methanobacteriales decreased linearly with increasing doses of MELM. In conclusion, the inclusion of MELM at a concentration of 2% DM in the substrate which could enhance cumulative gas production, NDF and true digestibility, C3 production, and microbial population, while reducing methane concentration and Methanobacterial abundance.
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Castillo-Lopez, Ezequias, Raul Rivera-Chacon, Sara Ricci, Nicole Reisinger i Qendrim Zebeli. "PSX-B-20 Effect of shifting from forage to high grain diet on chewing, feed sorting and lying behavior of cows supplemented with or without a phytogenic additive". Journal of Animal Science 99, Supplement_3 (8.10.2021): 219–20. http://dx.doi.org/10.1093/jas/skab235.399.
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Abstract The aim was to evaluate the effect of changing from forage to a high grain diet on chewing, feed sorting and lying behavior of cows supplemented with or without a phytogenic feed additive. Nine cows were blocked in two groups and used in a change-over design. Each experimental run consisted of one week of forage feeding, one week of diet transition, and four weeks of high grain feeding (65% concentrate). During the washout period of 10 weeks between the two runs, cows grazed on pasture. Cows were fed either a control diet (CON) or a diet with a phytogenic additive (PHY) based on menthol, thymol and eugenol. Data on chewing, feed sorting and lying behavior were analyzed using SAS with week of feeding and supplementation as fixed effects, and cow as random effect. Data across weeks from the same cow were processed using a first order variance-covariance structure matrix taking into account that the covariance decays with time. We found a reduction (P < 0.01) in rumination time by 53% with diet change. Within the high grain feeding, rumination reached lowest value in the second week (160 min/d). The high grain diet increased (P < 0.01) selection for long size particles by 20%. Although lying time was not affected immediately after diet change, it increased (P < 0.01) by 8% from the second week of high grain consumption. In the fourth week of grain feeding, there was a tendency for PHY to increase rumination (P = 0.07) and total chewing time (P = 0.10), but with no effect on sorting for fiber or lying time. The high grain diet decreased chewing and standing time, and increased sorting for fibrous ingredients especially after one week of feeding. The phytogenic feed additive may contribute to stimulate chewing in long term supplementation.
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Olgun, Osman. "The Effect of Dietary Essential Oil Mixture Supplementation on Performance, Egg Quality and Bone Characteristics in Laying Hens". Annals of Animal Science 16, nr 4 (1.10.2016): 1115–25. http://dx.doi.org/10.1515/aoas-2016-0038.
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Abstract The aim of this study was to investigate the effect of seven different levels (0, 25, 50, 100, 200, 400, and 600 mg/kg) of a phytogenic feed additive containing a mixture of essential oils from thyme, black cumin, fennel, anise and rosemary on performance, eggshell quality, bone biomechanical properties and bone mineralization in laying hens. This study consisted of a total of 112, 21-week-old Super Nick laying hens, which were randomly distributed into seven experimental groups. During the 12-week experimental period, each experimental group of four replicates of four birds each was fed with seven treatment diets. Egg weight and egg mass were positively linearly affected by essential oil mixture supplementation. Also, eggshell thickness was increased quadratically by essential oil mixture supplementation. The biomechanical properties and tibia mineral content were adversely affected by essential oil mixture supplementation at the level of 600 and 400 mg/ kg, respectively. These results demonstrated that dietary supplementation with a low or medium concentration of essential oils improved bone parameters, while at high levels were adversely affected in laying hens.
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Rivera-Chacon, Raul, Ezequias Castillo-Lopez, Sara Ricci, Renee M. Petri, Nicole Reisinger i Qendrim Zebeli. "Supplementing a Phytogenic Feed Additive Modulates the Risk of Subacute Rumen Acidosis, Rumen Fermentation and Systemic Inflammation in Cattle Fed Acidogenic Diets". Animals 12, nr 9 (6.05.2022): 1201. http://dx.doi.org/10.3390/ani12091201.
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Feeding with high-concentrate diets increases the risk of subacute ruminal acidosis (SARA). This experiment was conducted to evaluate whether supplementing a phytogenic feed additive based on L-menthol, thymol, eugenol, mint oil (Mentha arvensis) and cloves powder (Syzygium aromaticum) (PHY) can amend the ruminal fermentation profile, modulate the risk of SARA and reduce inflammation in cattle. The experiment was designed as a crossover design with nine non-lactating Holstein cows, and was conducted in two experimental runs. In each run, cows were fed a 100% forage diet one week (wk 0), and were then transitioned stepwise over one week (0 to 65% concentrate, wk adapt.) to a high concentrate diet that was fed for 4 weeks. Animals were fed diets either with PHY or without (CON). The PHY group had an increased ruminal pH compared to CON, reduced time to pH < 5.8 in wk 3, which tended to decrease further in wk 4, reduced the ruminal concentration of D-lactate, and tended to decrease total lactate (wk 3). In wk 2, PHY increased acetate, butyrate, isobutyrate, isovalerate, and the acetate to propionate ratio compared to CON. Phytogenic supplementation reduced inflammation compared to CON in wk 3. Overall, PHY had beneficial effects on ruminal fermentation, reduced inflammation, and modulated the risk of SARA starting from wk 3 of supplementation.
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Shehata, Awad A., Sakine Yalçın, Juan D. Latorre, Shereen Basiouni, Youssef A. Attia, Amr Abd El-Wahab, Christian Visscher i in. "Probiotics, Prebiotics, and Phytogenic Substances for Optimizing Gut Health in Poultry". Microorganisms 10, nr 2 (8.02.2022): 395. http://dx.doi.org/10.3390/microorganisms10020395.
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The gut microbiota has been designated as a hidden metabolic ‘organ’ because of its enormous impact on host metabolism, physiology, nutrition, and immune function. The connection between the intestinal microbiota and their respective host animals is dynamic and, in general, mutually beneficial. This complicated interaction is seen as a determinant of health and disease; thus, intestinal dysbiosis is linked with several metabolic diseases. Therefore, tractable strategies targeting the regulation of intestinal microbiota can control several diseases that are closely related to inflammatory and metabolic disorders. As a result, animal health and performance are improved. One of these strategies is related to dietary supplementation with prebiotics, probiotics, and phytogenic substances. These supplements exert their effects indirectly through manipulation of gut microbiota quality and improvement in intestinal epithelial barrier. Several phytogenic substances, such as berberine, resveratrol, curcumin, carvacrol, thymol, isoflavones and hydrolyzed fibers, have been identified as potential supplements that may also act as welcome means to reduce the usage of antibiotics in feedstock, including poultry farming, through manipulation of the gut microbiome. In addition, these compounds may improve the integrity of tight junctions by controlling tight junction-related proteins and inflammatory signaling pathways in the host animals. In this review, we discuss the role of probiotics, prebiotics, and phytogenic substances in optimizing gut function in poultry.
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Mesquita, Abner Alves, Poliana Carneiro Martins, Patrick Bezerra Fernandes, Leonardo Amorim de Oliveira, Paulo Victor Toledo Leão, João Antônio Gonçalves e. Silva, João Vitor Teixeira da Cunha i in. "Milk and Fresh Cheese Quality of Crossbred Cows Supplemented with Phytogenic Additives and Managed under Thermal Stress". Animals 13, nr 21 (2.11.2023): 3402. http://dx.doi.org/10.3390/ani13213402.
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This investigation aimed to assess the physiological parameters and quality of milk and fresh cheeses produced by cows that were housed in paddocks, either with or without shade, and supplemented with a phytogenic additive. Sixteen crossbred cows were allocated in a 4 × 4 Latin square design, dividing them into paddocks with or without shade, and providing or not providing a phytogenic additive in their feed. This resulted in a total of four treatment groups and sixteen experimental plots, each containing four animals, over four periods of 21 days. Various parameters were examined, including haematology, rectal and skin temperature, respiratory rate, milk yield and composition, serum parameters, and cheese yield and quality. It is worth noting that the temperature and humidity, as measured by a black globe thermometer, did not display significant variations between the different environments and exhibited minimal fluctuations throughout the day. Additionally, the supplementation of the phytogenic additive led to a reduction in haematocrit levels (p = 0.011). Furthermore, the analysis showed that whey obtained from cheese production had a higher fat content when cows were without access to shade (p = 0.005). Notably, there was an interaction between factors in relation to the total dry extract content, which was lower when cows had access to shade and received the additive (p = 0.010). In summary, the provision of a phytogenic additive and the presence or absence of shade did not bring about significant changes in milk production and quality or in the yield and quality of fresh cheese.
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Vega, Caroline Gonzalez, Sabrina May, Molly L. McGhee, Neil Paton i Kari Saddoris-Clemons. "140 Effects of Dietary Inclusion of a Medium Chain Fatty Acid Blend and a Phytogenic Feed Additive on Growth Performance, Gut Health, and Mortality of Escherichia Coli-Challenged Pigs". Journal of Animal Science 101, Supplement_2 (28.10.2023): 92–93. http://dx.doi.org/10.1093/jas/skad341.103.
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Abstract An experiment was conducted to test the hypothesis that supplementing diets with a blend of medium chain fatty acids (MCFA) and a phytogenic feed additive (Fresta Protect, Delacon, Linz, Austria), together or separately, would improve growth performance and stool quality of nursery pigs challenged with Escherichia coli (ETEC). A total of 200 pigs [5.52 ± 0.17 kg body weight (BW)] were weaned at 17 to 19 days of age, allotted to 40 pens (5 pigs/pen), and allowed to acclimate for 2 days before initiation of dietary treatments. Pens were blocked by BW and randomly assigned to 1 of 5 treatments: negative control (NC), NC + 55 mg/kg carbadox (Positive control; PC), NC + 1.0% MCFA, NC + 0.1% phytogenic, or NC + 1.0% MCFA and 0.1% phytogenic. All pigs were administered an oral gavage of 5 mL ETEC (blend of K88+ and F18+) with a final titer of 1010 CFU/pig on day 5 and 6. Body weights were recorded on days 0, 4, 11, and 18, whereas feed intake was recorded for the overall trial (day 0 - 18). Fecal scores were assessed daily on an ordered likert scale to determine the joint probability of more normal stool quality. Data were analyzed using the lme4 package of R 4.1.2, and the statistical models included the fixed effect of treatment and random effect of BW block. Growth performance data were assumed to be normally distributed, whereas mortality and stool quality were analyzed using generalized linear mixed models specifying binomial and multinomial distributions, respectively. Contrasts were used to test for effects of dietary additive (carbadox, MCFA, phytogenic, or MCFA and phytogenic) compared with NC (Table 1). Results indicated post-challenge BW, post-challenge and overall average daily gain (ADG), and overall gain:feed (G:F) were greater (P < 0.05) for PC than for NC. In the pre-challenge phase, dietary inclusion of MCFA reduced (P < 0.05) final BW and ADG compared with NC, but overall G:F was greater (P < 0.05) than NC when the MCFA blend was fed. Inclusion of the phytogenic increased (P < 0.05) ADG in the post-challenge period and G:F in the overall trial compared with NC. The combination of MCFA blend and phytogenic resulted in increased (P < 0.05) post-challenge final BW, post-challenge ADG, and overall ADG compared with NC. All treatments, except MCFA blend, tended (P < 0.10) to reduce mortality compared with NC. The PC, MCFA blend, and combination treatments resulted in a greater (P < 0.05) probability of normal stool quality than NC. In conclusion, supplementation of the phytogenic and the combination of MCFA with phytogenic improved growth performance and tended to reduce mortality of nursery pigs under ETEC challenge. Furthermore, the combination improved stool quality.
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Cervantes, Miguel, Panagiotis Sakkas, Moisés Soto, Alejandra Jaquelin Gómez, Reyna L. Camacho, Néstor Arce, Nicolas Quilichini i Adriana Morales. "A Capsaicin-Based Phytogenic Solution Improves Performance and Thermal Tolerance of Heat-Stressed Growing Pigs". Animals 14, nr 6 (21.03.2024): 973. http://dx.doi.org/10.3390/ani14060973.
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Exposure to heat stress (HS) detrimentally affects pig performance. This study explored whether a dietary phytogenic solution based on Capsicum spp. (PHY) could enhance the thermal tolerance of heat-stressed growing pigs. Forty-two individually housed pigs were randomly assigned to three treatments: thermoneutral pigs on a control diet (TN-C) and pigs subjected to HS fed the control diet either without (HS-C) or with supplemental PHY (HS-PHY). The TN-C group exhibited increased average daily gain (ADG) and feed intake (FI) compared to both HS-C (p < 0.01) and HS-PHY pigs (p < 0.05) and better feed efficiency compared to HS-C pigs only (p < 0.01). However, the HS-PHY pigs showed significantly higher FI (p < 0.01) and ADG (p < 0.05) compared to HS-C pigs. HS pigs displayed higher body temperatures (BTs) than TN pigs (p < 0.01), yet HS-PHY pigs experienced a lesser increase in BT compared to HS-C pigs (p < 0.05). Supplementation with PHY mitigated some effects of HS, increasing serum superoxide dismutase and catalase activity, reducing HSP90 expression in longissimus dorsi muscle, and elevating jejunal villus height compared to HS-C pigs (p < 0.05), reaching levels akin to TN-C pigs. Additionally, PHY supplementation resulted in lower serum urea levels than HS-C pigs (p < 0.01) and similar myosin gene expression to TN-C pigs (p > 0.1), suggesting enhanced amino acid post-absorptive utilization for lean tissue growth. In conclusion, dietary PHY supplementation partially offset the adverse effects of HS on pig performance by improving thermal tolerance.
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Cardoso, Verônica da Silva, Cristina Amorim Ribeiro de Lima, Marco Edílson Freire de Lima, Luis Eduardo Gomes Dorneles i Maria das Graças Miranda Danelli. "Piperine as a phytogenic additive in broiler diets". Pesquisa Agropecuária Brasileira 47, nr 4 (kwiecień 2012): 489–96. http://dx.doi.org/10.1590/s0100-204x2012000400003.
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The objective of this work was to determine the effect of piperine as a phytogenic additive in chicken broiler diet. Seven‑day‑old male chicks were randomly allocated in four experimental treatments (n = 24), with four replicates (n = 6). The piperine was added to diets at concentrations of 0, 60, 120, and 180 mg kg‑1 for 35 consecutive days. The following were evaluated: biochemical, hematological and histopathological parameters; performance and carcass yield. Histomorphometric analyses were also carried out. The addition of 120 and 180 mg kg‑1 of piperine did not alter broiler body weight and feed conversion, whereas 60 mg kg‑1 of piperine interfered positively in both parameters from 36 to 42 days of age and significantly increased the absorption surface of the duodenum and the ileum. No macroscopic alteration in organ size and color was observed in the broilers fed diets with the evaluated concentrations of piperine. The supplementation of 120 and 180 mg kg‑1 of piperine is toxic to liver tissue and reduces the absorption surface of the jejune. The diet supplemented with 60 mg kg‑1 of piperine is safe.
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Flees, Joshua J., Nima K. Emami, Elizabeth Greene, Bhaskar Ganguly i Sami Dridi. "Phytogenic Water Additives Improve Broiler Growth Performance via Modulation of Intermediary Metabolism-Related Signaling Pathways". Animals 11, nr 3 (9.03.2021): 750. http://dx.doi.org/10.3390/ani11030750.
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A ban on the use of antibiotic growth promoters (AGPs) has fueled and promoted scientific research towards the identification of reliable and effective alternatives. The supplementation of phytogenics AV/SSL12 (AVSSL) and Superliv Gold (SG) in water has been shown to improve broiler feed efficiency (FE) via modulation of hypothalamic neuropeptides. However, their effects on peripheral metabolic pathways are still unknown. The present study was undertaken to determine the effects of AVSSL and SG on lipid and protein metabolism-associated pathways in various tissues. Day-old male Cobb 500 chicks (n = 288) were randomly assigned to 3 treatment groups, with 8 replicates of 12 birds each. The treatment groups were fed a basal diet and supplemented with AVSSL or SG in the drinking water at a rate of 2, 4, and 7 mL/100 birds/d during the starter, grower, and finisher phases, respectively. The control group were fed a basal diet with no additive supplementation. On d 35, liver, adipose, and muscle tissue were collected from one bird per pen (8 birds/group). Data were analyzed using Student’s T-test to compare one treatment group to the control using Graph Pad Prism version 6.0 for Windows. In the liver, the levels of phosphorylated acetyl-CoA carboxylase alpha (ACCα) were significantly increased in both the AVSSL and SG groups compared to the control. The hepatic expression of sterol regulatory element-binding protein cleavage-activating protein (SCAP) was significantly downregulated in both treated groups compared to the control. AVSSL supplementation downregulated the hepatic expression of SREBP-2 and adiponectin (AdipoQ), while SG administration upregulated hepatic AdipoR1/R2 mRNA abundances compared to the untreated group. Both AVSSL and SG treatments upregulated hepatic stearoyl-CoA desaturase-1 (SCD-1) gene expression compared to their untreated counterparts. In the adipose tissue, the levels of phosphorylated hormone-sensitive lipase (HSL) at Ser855/554 site were increased in both the AVSSL and SG groups compared to the control. However, ATGL protein expression was decreased in SG compared to the untreated group. In the muscle, the levels of phosphorylated mechanistic target of rapamycin (mTOR) were increased in the AVSSL, but decreased in the SG group compared to the control. Collectively, these data indicate that supplementation of the phytogenics AVSSL and SG in water reduced hepatic lipogenesis-related proteins and increased adipose tissue lipolysis- and muscle protein synthesis-associated targets, which might explain, at least partially, the improvement in FE observed in previous research.
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Mihaela, Saracila, Panaite Tatiana Dumitra, Papuc Camelia Puia, Predescu Corina Nicoleta i Untea Arabela. "Dietary phytogenic mixture for broilers reared under thermoneutral and heat stress conditions". Archiva Zootechnica 23, nr 2 (1.12.2020): 101–16. http://dx.doi.org/10.2478/azibna-2020-0016.
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Abstract During two feeding trials, the effect of dietary phytogenic mixture on the performance and oxidative stress biomarkers in the liver of broilers reared under thermoneutral conditions (TN) and heat stress (HS) was studied. A number of 60 Cobb 500 chicks/trial were sheltered in environmentally-controlled digestibility cages. On the 14 days of age, the chicks were weighted and assigned to four groups (2 groups/trial with 30 chicks/group). In the first trial, two groups (C-TN and PM-TN) were kept in thermoneutral conditions. In the second trial, other two groups were kept (C-HS and PM-HS) in heat stress (32 ±1 °C). The structure of diets was the same in both experiments. Compared with the control diet (C), the experimental diet (PM) contained the addition of 1% phytogenic mixture (bilberry leaves, peppermint leaves, fennel leaves and sea buckthorn meal). Irrespective of temperature conditions, dietary PM did not affect broiler’s performance. The dietary supplementation of PM delayed protein and lipid oxidation in the liver tissue of broilers in both trials by increasing the hepatic catalase, glutathione and superoxide dismutase activity.
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Bajagai, Yadav S., Friedrich Petranyi, Darwin Horyanto, Romeo Batacan, Edina Lobo, Xipeng Ren, Maria M. Whitton, Sung J. Yu, Advait Kayal i Dragana Stanley. "Ileum transcriptional response to prolonged supplementation with phytogenic product containing menthol, carvacrol and carvone". Heliyon 8, nr 3 (marzec 2022): e09131. http://dx.doi.org/10.1016/j.heliyon.2022.e09131.
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Khader, Nasser, tahia ahmed i Aya Ahmed. "Effect of dietary supplementation of phytogenic feed additives on broiler performance and economic efficiency". Benha Veterinary Medical Journal 40, nr 2 (1.07.2021): 137–43. http://dx.doi.org/10.21608/bvmj.2021.73282.1400.
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Nieman, David C., Courtney L. Capps, Christopher R. Capps, Zack L. Shue i Jennifer E. McBride. "Effect of 4-Week Ingestion of Tomato-Based Carotenoids on Exercise-Induced Inflammation, Muscle Damage, and Oxidative Stress in Endurance Runners". International Journal of Sport Nutrition and Exercise Metabolism 28, nr 3 (1.05.2018): 266–73. http://dx.doi.org/10.1123/ijsnem.2017-0272.
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This double-blind, randomized, placebo-controlled crossover trial determined if ingestion of a supplement containing a tomato complex with lycopene, phytoene, and phytofluene (T-LPP) and other compounds for 4 weeks would attenuate inflammation, muscle damage, and oxidative stress postexercise and during recovery from a 2-hr running bout that included 30 min of −10% downhill running. Study participants ingested the T-LPP supplement or placebo with the evening meal for 4 weeks prior to running 2 hr at high intensity. Blood samples and delayed onset muscle soreness ratings were taken pre- and post-4-week supplementation, and immediately following the 2-hr run, and then 1-hr, 24-hr, and 48-hr postrun. After a 2-week washout period, participants crossed over to the opposite treatment and repeated all procedures. Plasma lycopene, phytoene, and phytofluene increased significantly in T-LPP compared with placebo (p < .001 for each). Significant time effects were shown for serum creatine kinase, delayed onset muscle soreness, C-reactive protein, myoglobin, 9- and 13-hydroxyoctadecadienoic acids, ferric reducing ability of plasma, and six plasma cytokines (p < .001 for each). The pattern of increase for serum myoglobin differed between T-LPP and placebo (interaction effect, p = .016, with lower levels in T-LPP), but not for creatine kinase, delayed onset muscle soreness, C-reactive protein, the six cytokines, 9- and 13-hydroxyoctadecadienoic acids, and ferric reducing ability of plasma. No significant time or interaction effects were measured for plasma-oxidized low-density lipoprotein or serum 8-hydroxy-2′-deoxyguanosine. In summary, supplementation with T-LPP over a 4-week period increased plasma carotenoid levels 73% and attenuated postexercise increases in the muscle damage biomarker myoglobin, but not inflammation and oxidative stress.
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Moreira, Janaina Da Silva, José Henrique Stringhini, Edemilson Cardoso da Conceição, Emmanuel Arhnold, Raiana Almeida Noleto i Fabyola Barros de Carvalho. "Supplementation of extract of Lafoensia pacari in the diet of semi heavy laying hens". Acta Scientiarum. Animal Sciences 39, nr 3 (14.07.2017): 281. http://dx.doi.org/10.4025/actascianimsci.v39i3.32209.
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It was intended to evaluate the supplementation of Lafoensia pacari standardized in tannins extract in the diet of laying hens on the performance, internal and external quality of eggs and metabolism of the feed nutrients. A total of 168 Isa Brown laying hens, aged 24 weeks, with the mean weight of 2.6 kg and the mean posture rate of 87% were used during 4 periods of 28 days each. The treatments consisted of Halquinol performance-enhancing antibiotic, Mannanoligosaccharide (MOS) prebiotic and three levels of pacari extract – 1,000, 2,000 and 3,000 mg kg-1of feed. The experimental design was completely randomized, with six treatments and seven replicates of four hens each. The pacari standardized in tannin extract presented a percentage of albumen and an egg weight similar to the antibiotic (p < 0.04). The supplementation with the extract improved the shell quality, verified by the specific gravity (p < 0.03) and promoted the metabolizability of ether extract similar to antibiotic and MOS (p < 0.04), allowing its indication as a phytogenic additive.
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Gheisar, Mohsen Mohammadi, Jin Young Cheong, Pinyao Zhao i In Ho Kim. "Evaluating the influence of dietary phytogenic blends on gestating and lactating sows and suckling piglets". Animal Production Science 58, nr 11 (2018): 2071. http://dx.doi.org/10.1071/an15447.
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This experiment was conducted to determine the effect of dietary inclusion of phytogenic blends on the performance of sows during the gestating and lactating periods and suckling piglets. Fifty-one multiparous sows (Landrace × Yorkshire) were randomly distributed into three treatments (17 sows per treatment): T1 (basal diet); T2 (T1 + 0.05% AC blend (Astragalus membranaceus and Chinese Liquorice); T3 (T1 + 0.05% SL blend (Scutellaria baicalensis and Lonicera japonica). Feeding the sows with diets supplemented with AC and SL blends decreased (P < 0.05) bodyweight loss after farrowing to weanling. The number of piglets, average daily feed intake, backfat thickness, and oestrus interval was not influenced (P > 0.05) by diets. Suckling piglets of sows fed with AC and SL blends showed higher (P < 0.05) weanling bodyweight and average daily gain weight. Piglet survival rate and birthweight were not affected by treatments. Feeding sows with AC and/or SL blends did not significantly influence the composition of colostrum or milk. There was not any significant impact on faecal score of suckling piglets. These results indicated that supplementation of phytogenic blends to the diet of sows during gestating and lactating period led to a decline in bodyweight loss after farrowing and could improve litter performance.
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Choubey, Mahipal, Ashok Kumar Pattanaik, Shalini Baliyan, Narayan Dutta, Sunil E. Jadhav i Kusumakar Sharma. "Dietary supplementation of a novel phytogenic feed additive: effects on nutrient metabolism, antioxidant status and immune response of goats". Animal Production Science 56, nr 10 (2016): 1612. http://dx.doi.org/10.1071/an14770.
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Fifteen adult Jamunapari goats (12–14 months; 18.83 ± 0.83 kg) were used to ascertain the effect of a phytogenic feed additive (PFA) on nutrient utilisation, metabolic profile, antioxidant status and immune response. The goats, randomly assigned to three groups, were fed a standard diet supplemented with the PFA at 0 (CON), 2 (PFA-2) and 3 (PFA-3) % of feed intake for a period of 60 days. Bodyweight and feed intake were recorded. At the end of the experiment, a metabolism trial was conducted. Blood was collected at fortnightly intervals and used to assess blood metabolites, select hormones and erythrocytic antioxidant status. Humoral and cell-mediated immune responses were also assessed. Although dietary use of PFA improved (P < 0.05) the digestibility of all the nutrients, supplementation of PFA did not impart any significant effect (P < 0.05) on dry matter intake or bodyweight changes. Nitrogen balance was higher (P < 0.05) in both the PFA groups compared with the CON. Most of the blood metabolites showed comparable values except for a lower (P < 0.05) aspartate aminotransferase activity in PFA-3. Serum cortisol reduced (P < 0.05) with PFA supplementation in a dose-dependent manner. The supplementation of PFA increased (P < 0.05) the activity of antioxidant enzymes viz. glutathione peroxidase, catalase and glutathione-S-transferase at higher doses (PFA-3), but did not alter the non-enzymatic antioxidant indices. There was no influence of PFA on the cell-mediated or humoral immune responses. Overall, the results indicated the potential of the PFA for improvement in nutrient utilisation and antioxidant status in adult goats.
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Younis, Mona, Yasmin Heikal i Mahmoud Ghanima. "Growth Performance, Cellular Immunity and Blood Chemical Analysis of Two Broilers Strains after Phytogenic Supplementation". Alexandria Journal of Veterinary Sciences 71, nr 1 (2021): 53. http://dx.doi.org/10.5455/ajvs.71135.
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Fascina, Vitor Barbosa, Guilherme Aguiar Mateus Pasquali, Daniella Aparecida Berto, Amanda da Lapa Silva, Edivaldo Antônio Garcia, Antonio Celso Pezzato, Elisabeth Gonzales i José Roberto Sartori. "Effects of arginine and phytogenic additive supplementation on performance and health of brown-egg layers". Revista Brasileira de Zootecnia 46, nr 6 (czerwiec 2017): 502–14. http://dx.doi.org/10.1590/s1806-92902017000600005.
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Talebi, Alireza, Masoud Maham, Siamak Asri-Rezaei, Pouya Pournaghi, Mohammad-Sadegh Khorrami i Amir Derakhshan. "Effects of Nigella sativa on Performance, Blood Profiles, and Antibody Titer against Newcastle Disease in Broilers". Evidence-Based Complementary and Alternative Medicine 2021 (14.06.2021): 1–15. http://dx.doi.org/10.1155/2021/2070375.
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Recent anxiety about resistance to chemical drugs has elevated the position of phytogenic feed additives including Nigella sativa in preventive strategy in the poultry industry. During this study, a completely randomized experiment was designed to investigate the efficacy of different levels (0 to 16%) of N. sativa seeds supplemented in the diet of broilers on performance, immune responses, and hematological and biochemical parameters. The results indicated the following: (a) Supplementation of 1% N. sativa seeds in diet had the highest positive effects and 16% N. sativa had the highest significant ( p = 0.03 ) adverse effects on weight gain, while up to 2% N. sativa seeds in the diet reduced feed conversion ratio (FCR) whereas 4% and over that increased the FCR. (b) Chickens fed with a diet containing 1% N. sativa seeds had the highest antibody titers, but those fed with 16% N. sativa seeds had the lowest antibody titers at end of the experiment. (c) Dietary inclusion of N. sativa seeds increased hemogram parameters and the group fed with 16% N. sativa seeds had the highest values on day 21 until the end of the experiment. (d) Supplementation of N. sativa seeds decreased in WBC and lymphocytes but increased heterophils, H/L, monocytes, eosinophils, and basophils percentages. Supplementation of up to 2% of N. sativa seeds in broiler’s diets elaborated serum level of those parameters, while supplementation of ≥ 4% N. sativa seeds decreased their serum levels. In conclusion, supplementation of N. sativa seed (1-2%) in broiler diets, as a multipurpose natural growth promoter, improves performance, elevates humoral immune responses, affects serum biochemical profiles of broiler chickens, and induces changes in their hemogram and leukogram, while there are no side, residual, and hazardous effects.
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Williams, Gabriel Adedotun, AYOTUNDE MAFIMIDIWO, WASIU OLAYEMI, Obafemi Foluso Akinjute, OLUWASEYI WILLIAMS i JOSHUA OGUNROMBI. "Effect of dietary supplementation with Ethiopian pepper (Xylopia aethiopica), cloves (Syzygium aromaticum), and their composite on growth performance, serum parameters, and haematological indices of broiler chickens". ANIMAL SCIENCE AND GENETICS 19, nr 1 (31.03.2023): 1–19. http://dx.doi.org/10.5604/01.3001.0016.2754.
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This study investigated the effect of dietary Ethiopian pepper (Xylopia aethiopica) and clove (Syzygium aromaticum) supplementation on growth performance, serum parameters and haematological indices of broiler chickens. Three hundred and sixty unsexed Ross broilers were used for the study. Four experimental diets were formulated for starter (0-28days) and finisher phases (29-56days); Diet 1 was the control without Phytogenic supplementation, Diet 2 (supplemented with 1% Ethiopian pepper (EP)), Diet 3 (supplemented with 1% Clove (CL)) and Diet 4 (supplemented with 1% mix of equal quantity (0.5 % each) of Ethiopian pepper and clove (EPCL)). Each treatment was replicated 6 times with 15 birds per replicate. Growth response was measured weekly for starter and finisher phase, blood was collected for serum and haematological parameters at the end of 4th and 8th week. Data obtained were subjected to one analysis of variance using SAS 2000 and significant means were separated using Tukeys test in same software. At day 28, dietary supplementation of EPCL increased (P<0.05) live weight (LW) (660.34 g), weight gain (WG) (599.66 g) with improved feed conversion ratio (FCR) (0.98). Total protein (TP) (48.00 g/L) and albumin (28.30 g/L) was higher (P<0.05) for broilers fed diet supplemented EPCL compared to other treatments. Packed cell volume (PCV) (30.00 %) was higher (P<0.05) in broilers fed diet with EPCL. Supplementation of CL and EPCL in the diet of broilers increased (P<0.05) red blood cell (RBC) count of broilers. At day 56, LW and WG was also increased (P<0.05) with EPCL supplementation. Reduced (P<0.05) cholesterol was observed for broilers fed diet supplemented EPCL. Haemoglobin (Hb) and RBC was higher (P<0.05) for broilers fed diet supplemented EPCL. It was concluded that EPCL can be supplemented in the diet of broilers for increased performance and improved blood parameters
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Blue, Candice E. C., Mallory B. White i Rami A. Dalloul. "Assessing the Effects of Phytogenic Feed Additives on Broilers during a Necrotic Enteritis Challenge". Poultry 3, nr 4 (28.09.2024): 346–53. http://dx.doi.org/10.3390/poultry3040026.
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Subclinical necrotic enteritis (NE) is an enteric disease that inflicts significant economic losses in the poultry industry, primarily by reducing performance in commercial flocks but without significant mortality. This study evaluated the effects of a variety of phytogenic blends on broilers’ performance and carcass composition during an induced NE challenge. In this study, 1120 day (d)-old male broilers were allocated to four treatments groups (14 replicate floor pens, 20 birds/pen): the control (CONT) group, fed a basal corn-soybean diet, and three phytogenic blend dietary additives (PHYTO1, PHYTO2, and PHYTO3) added to the basal diet at 150, 250, and 500 mg/kg feed, respectively. Subclinical NE was induced by spraying a concentrated coccidiosis vaccine onto the feed and litter 24 h post-placement. On day 8, two birds/pen were necropsied for NE lesions. On days 8, 14, 28, and 42, the average daily gain (ADG), feed intake (ADFI), and feed conversion ratio (FCR) were calculated. On day 42, two birds/pen were euthanized to assess carcass composition using dual-energy X-ray absorptiometry (DXA). Statistical analyses for all data were performed using the ANOVA procedure (JMP, Pro 16) and significance (p ≤ 0.05) between treatments was determined by the LSD test. There was no effect of treatment on NE lesions. PHYTO1, PHYTO2, and PHYTO3 significantly improved FCR from days 9 to 14, 0 to 14, and 0 to 42 and resulted in greater ADG from days 9 to 14, 29 to 42 and cumulatively on days 0 to 42. Carcass composition data revealed a numerically higher lean-to-fat ratio in the PHYTO groups compared to the CONT group. These results indicate that the dietary supplementation of phytogenic blends could alleviate the adverse effects of NE challenge on broilers’ performance and carcass composition.
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Orzuna-Orzuna, José Felipe, Alejandro Lara-Bueno, Adrián Gloria-Trujillo, Germán David Mendoza-Martínez, Luis Alberto Miranda-Romero i Pedro Abel Hernández-García. "Growth Performance, Dietary Energetics, Blood Metabolites, Carcass Traits, Meat Quality, and Gene Expression of Lambs Supplemented with a Polyherbal Phytogenic Additive". Veterinary Sciences 11, nr 11 (25.10.2024): 520. http://dx.doi.org/10.3390/vetsci11110520.
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This study aimed to evaluate the effects of supplementation with a polyherbal phytogenic additive (PPA) on the productive performance, dietary energetics, blood metabolites, carcass traits, meat quality, and gene expression of finishing lambs. Thirty-six male Pelibuey lambs (23.61 ± 0.57 kg body weight (BW)) were housed in individual pens and assigned to four treatments (n = 9) with different doses of PPA: 0 (CON), 2.5 (PPAL), 5 (PPAM), and 7.5 (PPAH) g of PPA/kg of DM for 56 days. Average daily gain, dry matter intake, and observed dietary net energy for maintenance and weight gain increased linearly (p < 0.05) in lambs supplemented with PPAH. A linear reduction (p = 0.02) in FCR was detected in lambs fed PPAM and PPAH. The PPAH supplementation linearly increased (p < 0.001) Longissimus dorsi muscle area, but other carcass traits were not affected (p > 0.05) by PPA doses. The physicochemical characteristics of the meat and the hematological parameters of the lambs were not affected (p > 0.05) by the PPA doses. The glucose, uric acid, creatinine, and bilirubin serum concentrations decreased linearly (p < 0.05) in lambs supplemented with PPAM and PPAH. Gene ontology analyses showed that nine biological processes were modified (p < 0.05), including DNA replication, drug metabolism–cytochrome P450, oxidative phosphorylation, and chemical carcinogenesis–reactive oxygen species. In conclusion, high doses (7.5 g/kg DM) of PPA can improve growth performance and dietary energy utilization efficiency in finishing lambs. Likewise, gene expression analysis indicates that supplementation with high doses of PPA could improve energy production and antioxidant status in finishing lambs.
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Wang, Jinquan, Shengchen Su, Chasity Pender, Raj Murugesan, Basharat Syed i Woo Kyun Kim. "Effect of a Phytogenic Feed Additive on Growth Performance, Nutrient Digestion, and Immune Response in Broiler-Fed Diets with Two Different Levels of Crude Protein". Animals 11, nr 3 (11.03.2021): 775. http://dx.doi.org/10.3390/ani11030775.
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The aim of this experiment was to evaluate the effect of a phytogenic feed additive (PFA) on growth performance and nutrient digestibility of broilers fed corn and soybean meal-based diets containing two different levels of crude protein. A 2 × 2 completely randomized factorial arrangement (eight replicates/treatment, 30 birds/replicate) was conducted with a positive control (PC) and negative control (NC) containing crude protein at standard or reduced by 1.5% (equivalent to a reduction of 15 g/kg), respectively, and supplementation of PFA at 0 or 125 ppm of diet. There were no significant interactions found between PFA and CP levels in the current study. Main effect analysis showed that during 0–42 d of age NC diets decreased body weight gain (p < 0.05), but increased feed intake (p < 0.05) and feed conversion ratio (FCR, p < 0.01), whereas supplementation of PFA resulted in a lower FCR (p < 0.01). The ileal nutrient digestibility was reduced (p < 0.05) in the broilers fed a reduced protein diet at 21 d compared to the standard protein level group, but there were no effects for PFA levels. Similarly, supplementing PFAs showed no effects on digestive enzyme (Alkaline phosphatase, amylase, and lipase) activity in jejunal digesta and jejunal brush border enzyme (maltase, sucrase, and aminopeptidase) activity. Supplementation of PFA downregulated (p < 0.05) the mRNA expressions of cytochrome P450 1A and interleukin 6 in the ileum but had no effects on nutrient transporter genes in the jejunum. In conclusion, supplementation of PFA reduced broiler FCR during the whole grow-out period and positively regulated the immune responses in the ileum.
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Shanks, Paul A., Eric Vanzant i James D. Caldwell. "PSXII-2 Potential interactions between prior endophyte exposure and phytogenic supplementation for finishing steers with varying chute exit velocities". Journal of Animal Science 98, Supplement_4 (3.11.2020): 446. http://dx.doi.org/10.1093/jas/skaa278.777.
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Abstract All-natural phytogenic supplementation has gained momentum in the feeding industry. Because mechanisms likely differ, these supplements could add benefit when combined with antibiotics in finishing cattle diets. Our objective was to determine influence of Actifor®Energy (ActEN) when fed with monensin and tylosin on performance and carcass measurements of finishing cattle with varying chute exit velocities (EV) and differing prior endophyte exposure (E). Crossbred steers (n = 118; 345 ± 33 kg BW) were utilized in a split plot design (whole plot: prior grazing toxic [EI] or nontoxic [EN] fescue × 6g·hd-1·d-1 dietary ActEN or control). Prior EV (PEV, subplot factor) from receiving, backgrounding, and grazing periods was included as a covariate. Weights and EV were recorded on d0, d28, d56, d112, and at slaughter when cattle were shipped to a commercial abattoir, harvested, and carcass measurements recorded. Starting weights were 22 kg lower (P = 0.10) for cattle previously grazing EI and 10 kg lower (P < 0.01) per 1m·s-1 increase in PEV. Intake (%BW) was unaffected (P > 0.10) by E or ActEN. Gain:feed (E×ActEN P = 0.09) was greater for EN/ActEN compared with EI/ActEN, most influenced by the first 28d. ADG was depressed by EI and increasing PEV (E×PEV P = 0.09) with greater PEV impact for EN compared with EI. Endophyte effects on ADG were most prominent in the first 56d. ActEN tended (P = 0.12) to interact with E status for ADG where gains were numerically greater for ActEN with EN compared with EI, especially during the first 28d. Carcass measurements were largely unaffected (P > 0.10) by E or ActEN. Decreased PEV (P ≤ 0.02) was associated with heavier HCW, increased backfat, and higher YG. Prior exit velocity and grazing regimen were the primary factors influencing performance and carcass traits. Indications of smaller effects of phytogenic supplementation on top of antibiotics, especially during the first 28 d of feeding, warrant further investigation.
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Aust, Olivier, Wilhelm Stahl, Helmut Sies, Hagen Tronnier i Ulrike Heinrich. "Supplementation with Tomato-Based Products Increases Lycopene, Phytofluene, and Phytoene Levels in Human Serum and Protects Against UV-light-induced Erythema". International Journal for Vitamin and Nutrition Research 75, nr 1 (1.01.2005): 54–60. http://dx.doi.org/10.1024/0300-9831.75.1.54.
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Carotenoids are suitable photoprotectants, and β-carotene supplements are used for protection against ultraviolet (UV) light-induced erythema. Protective effects are also observed when carotenoids are provided with the diet. Here, we investigated the photoprotective effects of synthetic lycopene in comparison with a tomato extract (Lyc-o-Mato®) and a drink containing solubilized Lyc-o-Mato® (Lyc-o-Guard-Drink). With these different sources, the volunteers ingested similar amounts of lycopene (about 10 mg/day). After 12 weeks of supplementation, significant increases in lycopene serum levels and total skin carotenoids were observed in all groups. Significant increases in the serum levels of phytofluene and phytoene occurred in the Lyc-o-Mato and the Lyc-o-Guard-Drink group. At weeks 0, 4, and 12 an erythema was induced with a solar light simulator. Dorsal skin of each subject was irradiated with 1.25 minimal erythemal dose (MED). Reddening of the skin was evaluated before and 24 hours after irradiation by chromametry and expressed as positive a-values (red/green-axis). Δ a-values (difference of a-value before irradiation and after 24 hours) were used as an index of erythema intensity. A decrease in the Δ a-value from week 0 to week 12, indicating prevention of erythema formation, was observed in all groups. Compared to week 0, the Δ a-value at week 12 was 25% lower in the synthetic lycopene group. The protective effect was more pronounced in the Lyc-o-Mato (38%) and Lyc-o-Guard-Drink (48%) groups. In the two latter groups, phytofluene and phytoene may have contributed to protection. Both of these carotenoids exhibit absorption maxima at wavelengths of UV light. Absorption of UV light protects skin from photodamage and might explain the differences observed between groups.
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Morsy, Tarek A., Ahmed E. Kholif, Moyòsore J. Adegbeye, Olurotimi A. Olafadehan, Gouda A. Gouda, Mahmoud Fahmy i Mireille Chahine. "Lupin Seed Supplementation as a Functional Feed Additive: In Vitro Ruminal Gas, Methane and Carbon Dioxide Production, Fermentation Kinetics, and Nutrient Degradability". Animals 14, nr 14 (20.07.2024): 2119. http://dx.doi.org/10.3390/ani14142119.
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The inevitable enteric gas emission from ruminants is considered a modern-day problem from an environmental perspective. Addressing this problem requires nutritional approaches such as the use of phytogenic additives in ruminant diets. In this regard, lupin seed (LS) can be a useful additive due to its phytochemical constituents. Therefore, this study investigated the effects of lupin (Lupinus angustifolius) seed supplementation as a functional and sustainable feed additive in sheep diet (50:50 concentrate-to-forage ratio) on in vitro gas production (GP; mL/g DM), methane (CH4; mL/g DM) and carbon dioxide (CO2; mL/g DM) emissions, fermentation parameters, and nutrient degradability (g/kg DM incubated). Gas production and CH4 were measured per gram of incubated dry matter (DM), degradable DM (dDM), degradable neutral detergent fiber (dNDF), and degradable acid detergent fiber (dADF). Lupin seeds were included at 0 (control), 0.5, 1.0, 1.5, and 2% of the diet. The seeds contained 3.27% essential oils (DM basis), with eucalyptol as the main phytochemical. The highest GP per gram of DM and dDM was observed (p < 0.01) with 2.0% LS supplementation level. While 1.0% LS had the highest GP per gram of dNDF, 0.5% lupin diet had the highest GP per gram of dADF. Asymptotic GP and CH4 emissions linearly and quadratically increased (p < 0.01) with increasing LS level, while lag time decreased. Despite increased CH4 production, the proportion of CH4 in total biogas was lower (p = 0.008) for LS treatments than the control, with the 0.5% LS showing the lowest CH4 proportion. Production of CO2 increased with lupin seed treatments, with 0.5% LS producing the highest proportion (p = 0.027). Degradability of DM, NDF, and ADF was greater (p < 0.01) for the high LS supplementation level, while 0.5% supplementation level decreased ADF degradability. Total short-chain fatty acids, acetic acid, and propionic acid increased (p < 0.05) with LS supplementation level, leading to a reduced acetate:propionate ratio. Rumen pH decreased (p = 0.036) with LS supplementation, while ammonia-N decreased (p = 0.045) and estimated metabolizable energy increased (p < 0.001) linearly. Calculated microbial protein synthesis (p = 0.005) and gas yield (p = 0.047) increased with LS supplementation level. LS supplementation at 2.0% of diet (DM basis) increased GP and CH4 emission (mL/g DM) and enhanced nutrient degradability, suggesting its potential use as a functional feed additive for ruminants when supplemented at a 2.0% level into diet.