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Moss, Amy F., Sonia Yun Liu i Peter H. Selle. "Progress in comprehending the phytate–phytase axis in chicken-meat production". Animal Production Science 58, nr 10 (2018): 1767. http://dx.doi.org/10.1071/an17594.
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After an extended delay, the level of acceptance of exogenous phytases by the global chicken-meat industry is now almost complete. Contemporary bacterial phytases degrade phytate primarily in the gizzard. The extent of phytate degradation determines the extent to which phytate-bound phosphorus (P) is liberated; however, studies designed to investigate phytate degradation along the digestive tract have generated some confusing outcomes. This may be related to the reactivity of the phytate moiety, coupled with problems with inert dietary markers and perhaps a lack of complete and uniform extractions of phytate from digesta due to variations in digesta pH and phytate solubility. Quite recently, phytase was shown to have profound impacts on sodium (Na) digestibility coefficients in four segments of the small intestine. This has obvious implications for intestinal uptakes of glucose and amino acids via their respective Na+-dependent transport systems and it is possible that phytate and phytase have reciprocal impacts on ‘sodium pump’ (Na+, K+-ATPase) activity. It has been recently demonstrated unequivocally that phytase has the capacity to increase amino acid digestibility coefficients to the extent that phytase may generate a ‘proximal shift’ in the sites of amino acid absorption. The impact of phytase on starch digestibility is more equivocal and phytase responses may stem more from enhanced glucose absorption rather than starch digestion. The acceptance of phytase is hardly surprising, given its capacity to increase P utilisation coupled with numerous other positive influences that are still being properly realised.
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Axambayeva, Altynay Seitkhanovna, i Alexander Vyacheslavovich Shustov. "RECOMBINANT THERMOTOLERANT PHYTASE PRODUCED IN E.COLI". CBU International Conference Proceedings 3 (19.09.2015): 412–18. http://dx.doi.org/10.12955/cbup.v3.631.
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Abstract: Phytic acid (myo-inositol hexakisphosphate) and its salts (phytates) are the major storage form of phosphorus in plants. Monogastric animals including hogs, poultry, and fish cannot utilize phytates as a source of phosphorus unless they are enzymatically destroyed with exogenous enzyme—phytase. Phytases are added to fodder in ever increasing dosage to improve utilization of plant-derived phosphorus because this reduces dependence of farms on inorganic fodder phosphates. Because of technological considerations, feed phytases have to withstand elevated temperatures (60-80°C), which are used during preparation of fodder. Enzymatic feed additives are becominutesg of high demand in Kazakhstan, and development of domestic technologies for production of agricultural enzymes is an ongoing challenge to the country’s biotechnology.Objectives: To develop a system for recombinant expression of industrially important thermotolerantphytase and confirm activity and thermal stability of the recombinantly expressed enzyme.Methods: De novo gene synthesis, expression of 6xHis-tagged protein in E.coli, immobilized metal affinity chouromatography, biochemical tests for activities of phosphatase and phytase.Results: Thermotolerantphytase was produced in E.coli using recombinant expression system. The obtained enzyme had phosphatise activity (hydrolyzed p-nitrophenyl phosphate) and phytase activity (hydrolyzed sodium phytate). The recombinant phytase tolerated increase of incubation temperature up to 70°C and demonstrated increase in activity towards phytate with increase in the reaction temperature in the range 30°C-70°C.Conclusion: Described gene and expression system have prospects of utilization in development of pilot industrial production of phytase in the country.
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Selle, Peter H., Shemil P. Macelline, Peter V. Chrystal i Sonia Yun Liu. "The Contribution of Phytate-Degrading Enzymes to Chicken-Meat Production". Animals 13, nr 4 (9.02.2023): 603. http://dx.doi.org/10.3390/ani13040603.
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The contribution that exogenous phytases have made towards sustainable chicken-meat production over the past two decades has been unequivocally immense. Initially, their acceptance by the global industry was negligible, but today, exogenous phytases are routine additions to broiler diets, very often at elevated inclusion levels. The genesis of this remarkable development is based on the capacity of phytases to enhance phosphorus (P) utilization, thereby reducing P excretion. This was amplified by an expanding appreciation of the powerful anti-nutritive properties of the substrate, phytate (myo-inositol hexaphosphate; IP6), which is invariably present in all plant-sourced feedstuffs and practical broiler diets. The surprisingly broad spectra of anti-nutritive properties harbored by dietary phytate are counteracted by exogenous phytases via the hydrolysis of phytate and the positive consequences of phytate degradation. Phytases enhance the utilization of minerals, including phosphorus, sodium, and calcium, the protein digestion, and the intestinal uptakes of amino acids and glucose to varying extents. The liberation of phytate-bound phosphorus (P) by phytase is fundamental; however, the impacts of phytase on protein digestion, the intestinal uptakes of amino acids, and the apparent amino acid digestibility coefficients are intriguing and important. Numerous factors are involved, but it appears that phytases have positive impacts on the initiation of protein digestion by pepsin. This extends to promoting the intestinal uptakes of amino acids stemming from the enhanced uptakes of monomeric amino acids via Na+-dependent transporters and, arguably more importantly, from the enhanced uptakes of oligopeptides via PepT-1, which is functionally dependent on the Na+/H+ exchanger, NHE. Our comprehension of the phytate–phytase axis in poultry nutrition has expanded over the past 30 years; this has promoted the extraordinary surge in acceptance of exogenous phytases, coupled with the development of more efficacious preparations in combination with the deflating inclusion costs for exogenous phytases. The purpose of this paper is to review the progress that has been made with phytate-degrading enzymes since their introduction in 1991 and the underlying mechanisms driving their positive contribution to chicken-meat production now and into the future.
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DULIŃSKI, Robert, Marek ZDANIEWICZ i Aneta PATER. "Effect of Phytase Addition to Buckwheat Wort on the Selected Fermentable Sugars, Polypeptide Profile and Nitrogen Content from Free Aminoacids". Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca. Food Science and Technology 78, nr 1 (16.05.2021): 33. http://dx.doi.org/10.15835/buasvmcn-fst:2020.0053.
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Relatively high levels of phytates in buckwheat malt and the low activity of endogenous phytases that limit the effective use of substrates for fermentation and yeast metabolism (starch, proteins, minerals) are an argument for using phytases in beer production technology. Two mash-in programs were applied: (1) the Congress program, typical for basic raw materials, (2) a program with temperature optimized for phytase activity. Commercial preparations of 3-phytase (Finase P) and 6-phytase (Ronozyme) were used in the study. Monitored levels of selected fermentable sugars indicates a statistically significant effect of phytase addition on the glucose content in both mash-in programs used. The SEC-HPLC chromatography allowed to select a key polypeptide with an estimated molecular weight of 40 kDa, whose relative peak area decreases as a result of the applied mash-increase treatment with phosphorolytic enzymes, although this relation was not statistically confirmed in the analysis of free amino acids content. The analyses carried out also indicate that apart from the target molecules, namely phytate and inositol, the use of phytases in the process of buckwheat wort preparation slightly changes the profile of fermentable sugars and causes significant changes in the polypeptide profile of the final mash.
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Reshetnichenko, O., V. Kryukov, P. Antonenko, L. Tarasenko, I. Glebova, S. Zinoviev, O. Piven, A. Antipov i R. Mylostyvyi. "Anti-nutritional effect of phytates – extraphosphoric effect of phytase". Tehnologìâ virobnictva ì pererobki produktìv tvarinnictva, nr 1(147) (29.05.2019): 6–23. http://dx.doi.org/10.33245/2310-9289-2019-147-1-06-23.
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The increase of animal and poultry production can be achieved by the effective use of fodder, including vegetable feed. However, it is known that the vegetable feed can contain anti-nutrients (phytic acid or its salts). They reduce the nutrient availability in the diet. That is why, the research aim is the description of the phytate influence on the body and the definition of the right feed phytases for fodder production. Phosphorus is responsible for the energy supply to the body for metabolic processes. It plays a significant role in the metabolism of proteins, fats and carbohydrates. It takes part in the synthesis of enzymes, hormones, vitamins. Phosphorus ensures the stability of the skeleton of animals along with calcium. However, the predominant part of phosphorus is not available for animals in the vegetable feed as it is presented by phytates, which do not split in the gastrointestinal tract (GIT) of animals. When phytates get into the acidic environment of the stomach they ionize and react with positively charged minerals, proteins, amino acids creating compounds that are inaccessible for further digestion. The availability of phosphorus from phytates is provided by the phytase adding to the fodder, which not only splits phytates, but also reduces their anti-nutritional effect by concentration decreasing. From the present-day data it is known that phytates contain difficult soluble phosphorus. They also make it difficult to absorb another biologically active nutrients from fodder. The enrichment of the animal diet with microbial phytase makes calcium, zinc and copper be more accessible. It improves digestibility of food and stimulates weight gain. Phytase activity determined by the laboratory method does not allow to make up a conclusion about its expected effectiveness for animals. At present it is almost impossible to conduct such complex research and to use the dynamic modeling of digestion processes in the laboratory. The decision about the appropriate use of proposed preparations with phytase in the fodder is made on the basis of the production test. Keywords: animal feed, enzymes, phytase, anti-nutritional effect of phytates, extra phosphoric effect of phytase, activity of the feed preparations of phytase.
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Suleimanova, Aliya, Daria Bulmakova i Margarita Sharipova. "Heterologous Expression of Histidine Acid Phytase From Pantoea sp. 3.5.1 in Methylotrophic Yeast Pichia Pastoris". Open Microbiology Journal 14, nr 1 (30.07.2020): 179–89. http://dx.doi.org/10.2174/1874285802014010179.
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Background and Objective: The major storage form of phosphorus in plant-derived feed is presented by phytates and not digested by animals. Phytases are able to hydrolyze phytates and successfully used as feed additives. Nevertheless, nowadays, there is a constant search of new phytases and expression systems for better production of these enzymes. In this study, we describe cloning and expression of gene encoding histidine acid phytase from Pantoea sp. 3.5.1 using methylotrophic yeast Pichia pastoris as the host. Methods: The phytase gene was placed under the control of the methanol-inducible AOX1 promoter and expressed in P. pastoris. Experiments of small-scale phytase expression and activity assays were used to test recombinant colonies. Four different signal peptides were screened for better secretion of phytase by P. pastoris. After 36 h of methanol induction in shake flasks, the maximum extracellular phytase activity (3.2 U/ml) was observed in P. pastoris strain with integrated construct based on pPINK-HC vector and Kluyveromyces maxianus inulinase gene signal sequence. This phytase was isolated and purified using affinity chromatography. Results: Recombinant phytase was a glycosylated protein, had a molecular weight of around 90 kDa and showed maximum activity at pH 4.0 and at 50°C. Recombinant phytase had excellent thermal stability – it retained high residual activity (100% ± 2%) after 1 hour of heat treatment at 70°C. Conclusion: The enhanced thermostability of the recombinant phytase, its expression provided by strong inducible promotor and the effectively designed expression cassette, the simple purification procedure of the secreted enzyme, and the possibility of large-scale expression make the foundation for further production of this bacterial phytase in P. pastoris at an industrial scale.
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Duliński, Robert, Marek Zdaniewicz, Aneta Pater, Dagmara Poniewska i Krzysztof Żyła. "The Impact of Phytases on the Release of Bioactive Inositols, the Profile of Inositol Phosphates, and the Release of Selected Minerals in the Technology of Buckwheat Beer Production". Biomolecules 10, nr 2 (21.01.2020): 166. http://dx.doi.org/10.3390/biom10020166.
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A relatively high concentration of phytate in buckwheat malt, and the low activity of endogenous buckwheat phytases, both of which limit the effective use of substrates (starch, proteins, minerals) for fermentation and yeast metabolism, gives rise to the potential for application of phytases in beer production. This study aims at obtaining a 100% buckwheat wort with high bioactive cyclitols (myo-inositol and D-chiro-inositol) concentrations released by exogenous phytases and acid phosphatases. Two mashing programs were used in the study, i.e., (1) typical for basic raw materials, namely the well-established Congress method, and (2) optimized for phytase activity. The results indicated a nearly 50% increase in the level of bioactive myo-inositol and an 80% degradation of phytate in the wort as a result of simultaneous application of phytase and phosphatase enzymes in the mashing of buckwheat malt. In addition, high D-chiro-inositol concentrations were released from malt to the buckwheat wort. The concerted action of the two phytases significantly increased (19–44%) Zn2+ concentrations in wort. This may be of great importance during mash fermentation by Saccharomyces cerevisiae yeasts. There is a potential to develop technology for buckwheat beer production, which, in addition to being free from gluten, comprises high levels of bioactive myo- and D-chiro-inositols.
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Suliasih, S. Widawati, A. Z. N. Ikhwani, Suyadi i I. M. Sudiana. "Phytase activity of phytase-producing bacteria isolated from mangrove sediment". IOP Conference Series: Earth and Environmental Science 976, nr 1 (1.02.2022): 012041. http://dx.doi.org/10.1088/1755-1315/976/1/012041.
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Abstract Through the role of phytase, some soil bacteria can mineralize insoluble organic P such as phytate (myoinositol hexakisphosphate). Phytase is a secreted enzyme possessing the ability to hydrolyze phytate into phosphate esters and inorganic P for plants absorption. This study aimed to isolate phytase-producing bacteria from mangrove sediment and examine the influence of nutrients (source of carbon, nitrogen, phosphorus) and physical conditions (temperature, pH, NaCl tolerance) on maximum phytase production. The presence of phytase activity was determined by examining the individual colonies for the formation of a clear zone. Furthermore, the isolates were screened qualitatively and quantitatively using solid and liquid phytase screening medium (PSM) containing sodium Phytate as substrates. The result showed that a total of 48 isolates have the potential to produce phytase with a production range of 1.11 - 14.83U/mL. The isolate F15 as Bacillus altitudinis was found to produce the highest phytase after 72 hours of incubation, was selected for further analysis. This strain resulted in optimal phytase levels at 35°C and a pH of 6.5 in physical parameters, tolerated 5% NaCl in the presence of lactose and tryptone, which served as carbon and nitrogen sources, respectively.
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Chuiko, N. V., A. Yu Chobotarov i I. K. Kurdish. "Abiotic Factors Influence on Bacillus subtilis IMV B-7023 Phytase Activity". Mikrobiolohichnyi Zhurnal 84, nr 6 (28.02.2023): 3–9. http://dx.doi.org/10.15407/microbiolj84.06.003.
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Bacteria of the Bacillus genus can synthesize specific phytase enzymes. This property is especially important for soil bacteria. It helps to mineralize phytin and phytates and to provide these bacteria and plants (in the root zone of which they live) with the available phosphorus. Our previous studies have demonstrated that the Bacillus subtilis IMV B-7023 strain exhibits a phytase activity and can use phytate as a nutrition source. It is a component of the «Azogran» complex bacterial preparation for crop production. As known, abiotic environmental factors can influence the phytase activity of bacteria. In particular, the phytase activity changes significantly under different pH and temperatures. Solid soil particles, including nanosized minerals, can also influence bacteria’s enzymatic activity. The influence of abiotic factors on Bacillus subtilis IMV B-7023 phytase activity has not previously been studied, so this was the aim of our research. Methods. The phytase activity of bacteria was studied by measuring the amount of phosphate released from sodium phytate during the enzymatic reaction, and the nanomaterials’ influence on growth — by cultivation methods. Results. The highest B. subtilis IMV B-7023 phytase activity was observed at 28°C. Also, there was no B. subtilis IMV B-7023 phytase activity at pH 4—6. However, this activity increased at pH 7 and did not change significantly with increasing the buffer system pH to 12. Silicon dioxide influence on the B. subtilis IMV B-7023 growth activity during cultivation in a media with phytate as a phosphorus source depended on the nanomaterial concentration. Thus, at 0.05 and 0.5 g/L of silicon dioxide in the medium, this strain growth activity increased by 8—18%, and at 5.0 g/L of these nanoparticles, bacteria growth inhibition by 19% was observed. At the same time, clay mineral bentonite did not affect the B. subtilis IMV B-7023 growth under the studied cultivation conditions. In addition, silicon dioxide and bentonite stimulated B. subtilis IMV B-7023 phytase activity at all studied concentrations. So, phytase activity increased by 1.82—3.34 times upon adding silicon dioxide and by 2.54—5.83 times upon adding bentonite into the medium. Since the optimal values for phytase activity of most genus Bacillus bacteria are within neutral pH values and temperatures within 50—55°C, a property of B. subtilis IMV B-7023 to show maximum phytase activity at alkaline pH and lower temperatures (28°C) and also stimulation of this activity by soil minerals increases competitiveness of this strain as a component of a bacterial preparation for crop production. Conclusions. Abiotic environmental factors influence the B. subtilis IMV B-7023 supernagrowth and phytase activity. Optimal physicochemical factors for the phytase activity of these bacteria are temperature 28°C and pH 7—12. The concentrations 0.05, 0.5, and 5.0 g/L of silicon dioxide and bentonite increase B. subtilis IMV B-7023 phytase activity. The effect of these nanoscale minerals on the B. subtilis IMV B-7023 growth depends on their type and concentration during cultivation in the medium with phytate as a phosphorus source. The obtained results indicate the potential ability of the B. subtilis IMV B-7023 strain to effectively assimilate phytates in neutral and alkaline soils, especially due to the interaction of these bacteria with bentonite and silicon dioxide nanoparticles. This expands the possibility of using B. subtilis IMV B-7023 in agricultural technologies.
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Jatuwong, Kritsana, Jaturong Kumla, Nakarin Suwannarach, Kenji Matsui i Saisamorn Lumyong. "Bioprocessing of Agricultural Residues as Substrates and Optimal Conditions for Phytase Production of Chestnut Mushroom, Pholiota adiposa, in Solid State Fermentation". Journal of Fungi 6, nr 4 (21.12.2020): 384. http://dx.doi.org/10.3390/jof6040384.
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Phytase is an enzyme that breaks down phytates to release phosphorus in an available form. This enzyme plays an important role in animals, especially monogastric animals. It serves to improve phytate digestion along with phosphorus absorption, which are required for optimal growth performance and health. In this study, five mushroom species (Amauroderma rugosum SDBR-CMU-A83, Ganoderma mastoporum SDBR-CMU-NK0244, Marusmius sp.1 SDBR-CMU-NK0215, Pholiota adiposa SDBR-CMU-R32 and Piptoporellus triqueter SDBR-CMU-P234) out of 27 mushroom species displayed positive phytase production by agar plate assay. Consequently, these five mushroom species were selected for determination of their potential ability to produce phytase under solid-state fermentation using five agricultural residues (coffee parchment, oil palm empty fruit bunches, rice bran, sawdust, and water hyacinth) as substrates. The highest yield of phytase production (17.02 ± 0.92 units/gram dry substrate) was obtained after one week of fermentation. Optimization for phytase production was determined by statistical approaches using a Plackett–Burman design to screen ten parameters of relevant substrate components. Two significant parameters, the amount of water hyacinth and the moisture content, were found to affect the production process of phytase. Furthermore, the optimal temperature, pH value, and fermentation period were evaluated. The results indicated that the highest degree of phytase production at 53.66 ± 1.68 units/gram dry substrate (3.15-fold increase) was obtained in water hyacinth containing 85% moisture content by addition with a suitable basal liquid medium at a pH value of 6.5 after being incubated at 30 °C for seven days. The crude phytase of P. adiposa was precipitated and the precipitated extract was then used to determine partial characterizations. The precipitated extract displayed high activities after exposure to conditions of 42 °C and pH 5.0. Furthermore, Fe2+ enhanced phytase activity and precipitated extract displayed the best stability at a pH value of 8.0 and a temperature of 4 °C.
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Abd El-Hack, Mohamed E., Mahmoud Alagawany, Muhammad Arif, Mohamed Emam, Muhammad Saeed, Muhammad A. Arain, Farman A. Siyal, Amlan Patra, Shaaban Saad Elnesr i Rifat Ullah Khan. "The uses of microbial phytase as a feed additive in poultry nutrition – a review". Annals of Animal Science 18, nr 3 (1.07.2018): 639–58. http://dx.doi.org/10.2478/aoas-2018-0009.
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Abstract Most of the phosphorus (P) in feed ingredients is present as phytate, which is poorly available for absorption in the gastrointestinal tract of different poultry species due to the lack of endogenous phytase. The supplementation of phytase increases the utilization of P by hydrolyzing phytate, which consequently may reduce the excretion of P in the environment. In addition, it has been suggested that phytase may improve the feed utilization, weight gain, egg production and egg traits, nutrient digestibility, energy availability, retention of important minerals in blood and bones. Thus, the effectiveness of phytase on performance and Ca and P absorption in layer chickens fed corn-soybean based diets has been well recognized. The current review briefly discusses the supplementation of phytase in the diet of poultry on performance and egg production and characteristics as well as amino acids and minerals availability.
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Hidayat, Cecep. "Utilization of Phytase to Overcome Phytic Acid in Broiler Diet". Indonesian Bulletin of Animal and Veterinary Sciences 26, nr 2 (2.02.2017): 057. http://dx.doi.org/10.14334/wartazoa.v26i2.1326.
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<p class="awabstrak2">Phytic acid has been considered as an antinutrient in broiler diet due to its strong chelator of divalent minerals. Phytic acid has ability for binding positively charged proteins, amino acids, and/or multivalent cations or minerals. The resulting complexes are insoluble, difficult to be hydrolyzed during digestion in poultry, and thus, nutritionally less available for absorption. The reduction of phytate activity can be carried out by phytase supplementation. The application of phytase with respect to animal feed supplement is reviewed in this paper. Application of phytase in broiler diet may liberate cations and other nutrients bound by phytate-P complexes resulting in improved production parameters in broilers. This is because phytase supplement increased nutrient (protein, minerals, amino acid, energy, and carbohydrate) digestibilities and availabilities. Overall, phytase could increase nutrient utilization in broiler, hence, increase the economic efficiency of broiler production and reduce the phosphor pollution to the environment.</p><p class="awabstrak3"> </p>
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Ries, Edi Franciele, i Gabriela Alves Macedo. "Improvement of Phytase Activity by a New Saccharomyces cerevisiae Strain Using Statistical Optimization". Enzyme Research 2011 (9.08.2011): 1–6. http://dx.doi.org/10.4061/2011/796394.
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Using statistical optimization, we enhanced the activity of phytase by a new Saccharomyces cerevisiae strain cultured in mineral medium. Concentrations of carbon source and inducer of phytase production were optimized using a 22 full factorial CCD and response surface methodology (RSM). Urea was fixed as nitrogen source in culture medium (0.15%, w/v). The culture medium consisting of 2.5% sucrose and 0.5% sodium phytate optimally supported the maximum phytase activity. In addition, we found that culture of the yeast at 35°C with shaking at 150 rpm supports maximum phytase production. The validity of this model was verified by culturing the organisms in flasks on a shaker. Using the optimized media and growth conditions, we obtained a 10-fold improvement in the production of phytase by S. cerevisiae.
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Mkumbe, Baraka Stewart, Sajidan Sajidan, Artini Pangastuti, Ari Susilowati i Ralf Greiner. "Production, Characterization, and Molecular Phylogenetic Analysis of Phytase from Aspergillus niger Isolates of an Indonesia Origin". HAYATI Journal of Biosciences 29, nr 4 (6.04.2022): 468–78. http://dx.doi.org/10.4308/hjb.29.4.468-478.
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This research aimed at analyzing the phytase from fungal isolates SB1, SB2, BS, and WF produced in cornstarch with glucose medium (CS+Glu) as carbon sources and Potatoes dextrose broth (PDB). The activity of phytase from isolates SB1, SB2, BS, and WF produced in CS+Glu medium was 2.97 UmL-1, 2.87 UmL-1, 3.18 UmL-1, and 4.37 UmL-1, respectively, while the activity of phytases was 2.07 UmL-1, 2.17 UmL-1, 2.22 UmL-1, and 2.78 UmL-1 respectively in PDB medium. The optimal temperature of SB1 and WF phytase was 40°C, while SB2 and BS were 50°C and 60°C, respectively. The optimal pH of SB1 and WF phytase was 5.0, while SB2 and BS phytase were 6.0, and 4.0 respectively. 18S rRNA gene analysis revealed that SB1 was 99% identical to Aspergillus niger ANTS (KY825168.1), SB2, BS, and WF were 99% identical to A. niger Moriga leaf (MG889596.1). Multiple sequences and phylogenetic analysis of phytase gene showed that phyA_SB1 and phyA_SB2 were 98% homology with A. ficuum (AAB26466), 97% with A. niger (ADP05107) while phyA_WF was 99% with A. ochraceoroseus (PLB29348), 98% with A. niger (ADP05105). The deduced proteins contain conserved motifs RHGARYPTD at N-terminal while lacking HD motif at C-terminal. These phytases were in the same cluster with Aspergillus sp. phytase A indicating that they belong to Histidine Acid Phosphatases (HAP) family.
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Menezes-Blackburn, Daniel, Nahad Al-Mahrouqi, Buthaina Al-Siyabi, Adhari Al-Kalbani, Ralf Greiner i Sergey Dobretsov. "Bacterial Communities Associated with the Cycling of Non-Starch Polysaccharides and Phytate in Aquaponics Systems". Diversity 13, nr 12 (30.11.2021): 631. http://dx.doi.org/10.3390/d13120631.
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Aquaponics are efficient systems that associate aquatic organisms’ production and plants by recirculating water and nutrients between aquaculture and hydroponic tanks. In this study, we characterised the bacterial communities in the freshwater aquaponics system that can mineralise polysaccharides and phytate by producing carbohydrate-degrading enzymes and phytases, by 16S rRNA gene sequencing and in vitro culture techniques. Around 20% of the operational taxonomic units (zOTUs) identified were previously reported to carry fibre-degrading enzyme putative genes, namely β-glucanase (1%), xylanase (5%), or cellulases (17%). Ten % of the zOTUs were previously reported to carry putative genes of phytases with different catalytic mechanisms, namely β-propeller (6%), histidine acid phytases (3%), and protein tyrosine phytase (<1%). Thirty-eight morphologically different bacteria were isolated from biofilms accumulated in fish and plant compartments, and identified to belong to the Bacilli class. Among these, 7 could produce xylanase, 8 produced β-glucanase, 14 produced cellulase, and 11 isolates could secrete amylases. In addition, Staphylococcus sp. and Rossellomorea sp. could produce consistent extracellular phytate-degrading activity. The PCR amplification of β-propeller genes both in environmental samples and in the isolates obtained showed that this is the most ecologically relevant phytase type in the aquaponics systems used. In summary, the aquaponics system is abundant with bacteria carrying enzymes responsible for plant-nutrient mineralisation.
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Odeniyi, Olubusola, i Leonard Itaba. "PRODUCTION AND CHARACTERIZATION OF AN Aspergillus niger GS1S6 PHYTASE CULTIVATED IN PHYTIC ACID-RICH AGRICULTURAL SUBSTRATES". Fungal Territory 3, nr 3 (13.07.2020): 9–15. http://dx.doi.org/10.36547/ft.2020.3.3.9-15.
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In animal farming, improvement of growth performance through successful nutrient uptake and digestibility is critical. Phytases hydrolyze the anti-nutritive phytic acid present in grains to lesser derivatives and release the phosphorus trapped therein. Twenty-eight fungi obtained from poultry droppings, cereal-rich soils and garden soils were screened for phosphate solubilization and phytase production using Pikovskaya agar and phytase screening medium. Out of the fungi, 61% were positive for phytase producing abilities and the most proficient, isolate GS1S6 with a solubilization index of 121%, was identified as Aspergillus niger GS1S6. Optimum phytase production was achieved at a fermentation period of 120 h, pH 5.5, glucose, (NH4)2SO4 using 72 hour old fungal inoculum. Mineral supplementation of natural agricultural substrates enhanced phytase production (1000U/L) the most in milled sesame seed medium. The ~31-38 KDa partially purified and characterized enzyme demonstrated optimum activities at 55oC and pH 6.0, while cationic inclusions reduced phytase activities. The Km and Vmax were low (1.308 mM and 0.077 mM/mL/min) from Linear-weaver plot with increasing substrate concentration implying that A. niger GS1S6 phytase may efficiently mineralize phytic acid and therefore hold great prospect for its commercialization.
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Sahara, Eli, Feni Despedia i Raden Ayu Aminah. "The Influence of Phytase Enzyme to Laying Performance and Quality of Egg Shell of Golden Arabian Chicken". E3S Web of Conferences 68 (2018): 01022. http://dx.doi.org/10.1051/e3sconf/20186801022.
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Poultry has low ability to digest dietary fibre and to process phytate. Rice bran is intensively used in poultry ration although it contains little bit higher in fibre and phytate. The purpose of this study was to find out the optimal level of phytase the enzyme that could break down phytate in the ration of Golden Arabian chicken to increase performance and physical quality of the eggs. This study used 90 golden arabian chickens (Golden Brakel Kriel), approximately five months of age. Individual cage with a wire floor was equipped with a feed and drinking troughs. Experimental ration was with a mixture of corn, rice bran, commercial concentrate, and mineral mixture. Phytase was added at the level of: zerro, without phytase = P1; 100 FTU/ kg of ration; 200 FTU/kgof ration = P2; 300 FTU/kg of ration =P3; 400 FTU/ kg of ration = P4; and 500 FTU/kg of ration = P5. Experimental design that used in this study was Complete Randomized Design (CRD) with six treatments and five replications and three golden arabian chickens per experimental unit. Results showed that the addition of phytase in golden arabian chickens ration improved the hen day production (P <0.05) up to 71.43% and increased the physical quality, especially weight of eggshell significantly (P <0.05) up to 4.516 gram (phytase enzyme 300 FTU/kg) . In conclusion, that the level of the phytase of 300 FTU/kg of ration was the most optimal dose to increase the daily egg production and improve the physical quality. However, the addition of phytase did not affect to feed consumption, feed efficiency, eggshell thickness and eggshell thickness index.
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Wyss, Markus, Luis Pasamontes, Arno Friedlein, Roland Rémy, Michel Tessier, Alexandra Kronenberger, Anke Middendorf i in. "Biophysical Characterization of Fungal Phytases (myo-Inositol Hexakisphosphate Phosphohydrolases): Molecular Size, Glycosylation Pattern, and Engineering of Proteolytic Resistance". Applied and Environmental Microbiology 65, nr 2 (1.02.1999): 359–66. http://dx.doi.org/10.1128/aem.65.2.359-366.1999.
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ABSTRACT Phytases (myo-inositol hexakisphosphate phosphohydrolases) are found naturally in plants and microorganisms, particularly fungi. Interest in these enzymes has been stimulated by the fact that phytase supplements increase the availability of phosphorus in pig and poultry feed and thereby reduce environmental pollution due to excess phosphate excretion in areas where there is intensive livestock production. The wild-type phytases from six different fungi, Aspergillus niger, Aspergillus terreus, Aspergillus fumigatus, Emericella nidulans, Myceliophthora thermophila, andTalaromyces thermophilus, were overexpressed in either filamentous fungi or yeasts and purified, and their biophysical properties were compared with those of a phytase from Escherichia coli. All of the phytases examined are monomeric proteins. WhileE. coli phytase is a nonglycosylated enzyme, the glycosylation patterns of the fungal phytases proved to be highly variable, differing for individual phytases, for a given phytase produced in different expression systems, and for individual batches of a given phytase produced in a particular expression system. Whereas the extents of glycosylation were moderate when the fungal phytases were expressed in filamentous fungi, they were excessive when the phytases were expressed in yeasts. However, the different extents of glycosylation had no effect on the specific activity, the thermostability, or the refolding properties of individual phytases. When expressed in A. niger, several fungal phytases were susceptible to limited proteolysis by proteases present in the culture supernatant. N-terminal sequencing of the fragments revealed that cleavage invariably occurred at exposed loops on the surface of the molecule. Site-directed mutagenesis of A. fumigatus andE. nidulans phytases at the cleavage sites yielded mutants that were considerably more resistant to proteolytic attack. Therefore, engineering of exposed surface loops may be a strategy for improving phytase stability during feed processing and in the digestive tract.
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Miao, Yu-Zhi, Hui Xu, Bao-Jin Fei, Dai-Rong Qiao i Yi Cao. "PCR–RFLP analysis of the diversity of phytate-degrading bacteria in the Tibetan Plateau". Canadian Journal of Microbiology 59, nr 4 (kwiecień 2013): 245–51. http://dx.doi.org/10.1139/cjm-2012-0752.
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Phytases play a very important role in increasing phytate digestion and reducing phosphorus pollution in the environment, and phytate-degrading bacteria have a ubiquitous distribution in the environment. Due to its extremely harsh environment, the Tibetan Plateau breeds possibly abundant, extreme microorganisms. In this research, 67 phytate-degrading bacteria were isolated from different habitats in the Tibetan Plateau. Among all isolates, 40.3% were screened from farmland, 25.3% from wetland, 4.5% from saline–alkaline soil, 7.5% from hot springs, and 22.4% from lawns, which showed that the distribution of the phytate-degrading bacteria varied with habitats. By the PCR–RFLP method, 16 different species were identified and named, 4 of which are reported for the first time as phytate-degrading bacteria, that is, Uncultured Enterococcus sp. GYPB01, Bacillaceae bacterium strain GYPB05, Endophytic bacterium strain GYPB16, and Shigella dysenteria strain GYPB22. Through the assay of phytase activity of 16 strains, Klebsiella sp. strain GYPB15 displayed the highest capability of phytase production. Through analysis of the optimum pH, the optimum temperature, and the thermal stability of enzyme from 16 strains, some especial phytate-degrading bacteria were obtained. Our findings clearly indicate a good relation between the composition of the soils from the different environments in the Tibetan Plateau and populations of cultivable phytate-degrading bacteria. Moreover, extreme harsh soils are logically the best soils in which to find some strains of phytate-degrading bacteria for exploiting in the fields of biotechnology and industry.
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Costa, Leonardo Emanuel de Oliveira, Thamy Lívia Ribeiro Corrêa, Janaina Aparecida Teixeira, Elza Fernandes de Araújo i Marisa Vieira de Queiroz. "Endophytic bacteria isolated from Phaseolus vulgaris produce phytases with potential for biotechnology application". Brazilian Journal of Biological Sciences 5, nr 11 (2018): 657–71. http://dx.doi.org/10.21472/bjbs.051105.
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Currently, endophytic microorganisms have become a good source of different enzymes and others metabolites of industrial interest. Among a huge spectral of molecules, enzymes as phytases have been emphasized by the ability to hydrolyze the phytic acid that represents the largest storage form of inorganic phosphorus in cereals, which are the staple diet of monogastric animals such as swine and poultry. Moreover, phytic acid acts as an antinutrient by chelating divalent metal ions, and it is interesting provide phytase as an animal feed supplement for those monogastric animals. In the current study, 158 endophytic bacteria isolated from the leaves of three cultivars of Phaseolus vulgaris were assessed for the ability to produce phytase. Among them, four isolates belonging to the Pseudomonas, Stenotrophomonas, Microbacterium and Rhodococcus genera were highlighted, due their phytase production. The phytase produced by Microbacterium foliorum BAC1157 exhibited activity at 70 oC and stability in the presence of divalent cations, indicating that this phytase has a promising use in the animal feed industry. To the authors' knowledge, this is the first report on phytase production by bacteria of the Microbacterium genera.
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Zinoviev, S., V. Kryukov, H. Mutieva, I. Glebova i N. Yarovan. "The anti-nutritional effect of phytates - the extraphosphorus effect of phytase (review)". Genetics and breeding of animals, nr 4 (3.01.2022): 74–84. http://dx.doi.org/10.31043/2410-2733-2021-4-74-84.
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An analytical review was conducted on the availability of phosphorus from phytates, which increase the inclusion of phytase in the feed, while not only phytic acid is broken down, but also as a result of a decrease in concentration reduces its anti-nutritional effect.Phosphorus from plant feeds is not fully available to animals, as it is part of phytates, the cleavage of which in the gastrointestinal tract (gastrointestinal tract) of animals is limited. Phytates, getting into the acidic environment of the stomach, ionize and react with positively charged minerals, proteins, amino acids, creating compounds inaccessible for further digestion. The inclusion of phytase in compound feed is accompanied by an extra phosphoric effect, which is expressed in an increase in the availability of amino acids and energy. The decision on the feasibility of including phytase in feed is made on the basis of production tests of the proposed drugs.The studies carried out by the authors of invitro confirm the assumption that phytates enter into chemical interactions with peptides, as well as amino acids formed in the processes of protein digestion, turning them into compounds inaccessible to absorption. The proposed explanation does not affect the results of the balance experiment, but allows us to develop the study of the mechanism in terms of the interaction of digested amino acids with phytates. Based on this, another conclusion can be seen: the destruction of phytates should occur in the body before the digestion and dissolution of protein. In birds, this place is the goiter and stomachs, in pigs - the stomach. The hydrolysis of newly formed phytates (FC-amino acid) requires phytases that are active in the environment of the small intestine at pH 6 -7. As a result, the bound amino acids will be re-released and available for absorption. The development of new phytase preparations, taking into account the proposed explanation, will make it possible to create more effective feed preparations.
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Vijayaraghavan, Ponnuswamy, R. Raja Primiya i Samuel Gnana Prakash Vincent. "Thermostable Alkaline Phytase from Alcaligenes sp. in Improving Bioavailability of Phosphorus in Animal Feed: In Vitro Analysis". ISRN Biotechnology 2013 (13.02.2013): 1–6. http://dx.doi.org/10.5402/2013/394305.
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A bacterial isolate, Alcaligenes sp. secreting phytase (EC 3.1.3.8), was isolated and characterized. The optimum conditions for the production of phytase included a fermentation period of 96 h, pH 8.0, and the addition of 1% (w/v) maltose and 1% (w/v) beef extract to the culture medium. This enzyme was purified to homogeneity and had an apparent molecular mass of 41 kDa. The optimum pH range and temperature for the activity of phytase were found to be 7.0-8.0 and 60°C, respectively. This enzyme was strongly inhibited by 0.005 M of Mn2+, Mg2+, and Zn2+. In vitro studies revealed that the phytase from Alcaligenes sp. released inorganic phosphate from plant phytates. Phytase released 1930 ± 28, 1740 ± 13, 1050 ± 31, 845 ± 7, 1935 ± 32, and 1655 ± 21 mg inorganic phosphate/kg plant phytates, namely, chick pea, corn, green pea, groundnut, pearl pea, and chick feed, respectively.
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Jing, Mingyan, Shusheng Zhao, Anna Rogiewicz, Bogdan A. Slominski i James D. House. "Effects of phytase supplementation on growth performance, plasma biochemistry, bone mineralisation and phosphorus utilisation in pre-lay pullets fed various levels of phosphorus". Animal Production Science 61, nr 6 (2021): 568. http://dx.doi.org/10.1071/an20265.
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Context Reducing the environmental impact of animal production is becoming a really hot topic, especially with raised concerns over excessive flows of nitrogen and phosphorus (P) to the environment. Aims The present study was conducted to determine the effects of phytase supplementation on growth, plasma biochemistry, bone mineralisation and P utilisation of pre-lay pullets fed varying levels of non-phytate P. Methods A total of 240 Lohmann pullet chicks were randomly allocated to one of six dietary treatments with eight replicate cages (5 birds per cage) per treatment. Six treatments included three phytase-free diets and three diets supplemented with 1000 U/kg phytase; the non-phytate P levels were 2.75–2.50–2.25, 3.75–3.50–3.25 and 4.75–4.50–4.25 g/kg in the former, and 1.75–1.50–1.25, 2.75–2.50–2.25 and 3.75–3.50–3.25 g/kg in the latter, for the age of 0–4, 4–8 and 8–16 weeks respectively. Key results No significant differences were found for growth performance, plasma biochemistry (calcium, P, alkaline phosphatase and albumin) and bone mineralisation among dietary treatments, but P retention (%) was different (P < 0.001). Analysis of planned contrasts showed that phytase supplementation increased phytate P retention (P < 0.001), and improving the utilisation of phytate P tended most efficiently under low P conditions. Total P retention rate was reduced slightly by phytase supplementation (P < 0.05). Conclusions The results indicated that dietary non-phytate P level could possibly be reduced to 1.75, 1.50 and 1.25 g/kg for 0–4, 4–8 and 8–16 weeks of age respectively after phytase supplementation, without compromising pullet growth and performance during the pre-laying period. Implications The results of this study will contribute to decreasing P excretion by poultry and reducing the potential environmental impact with land application of manure.
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G. A., Gowthami, i Gunashree B. S. "Isolation, characterization and optimization of Cystobasidium minutum for phytase production". Biomedicine 43, nr 01 (28.03.2023): 329–34. http://dx.doi.org/10.51248/.v43i01.2654.
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Introduction and Aim: Phytic acid (myo-inositol 1, 2, 3, 4, 5, 6- hexakis dihydrogen phosphate), a storage form of phosphorus is present in legumes, cereals and oilseeds. By forming insoluble complexes with proteins and divalent cations, it serves as an anti-nutrient in animal feed. Phytase (EC 3.1.3.8), a special class of phosphomonoesterases, catalyse the conversion of inorganic phosphate into mono-, di-, tri-, tetra-, and penta-phosphates of derivatives of myo-inositol. It is an important enzyme in food and feed industries. The present study aimed at isolation, molecular identification and optimization of physico-chemical parameters for phytase production by a red pigmented yeast Cystobasidium minutum.
Materials and Methods: The phytase activity was estimated by using sodium phytate as substrate and the production of phytase under varied temperature, pH, agitation, incubation time, carbon and nitrogen sources.
Results: The results showed maximum activity of 91.86 and 27.13 U/ml at 35°C and pH 5.5. At an agitation speed of 150 rpm and 120h of incubation time the enzyme activity was 18.9 and 21.4 U/ml respectively. Among the carbon sources tested sucrose served for highest enzyme activity of 26.33 U/ml and ammonium sulphate served the sole source of nitrogen and showed an activity of 50.12 U/ml.
Conclusion: Cystobasidium minutum (Rhodotorula minutum) produced maximum phytase enzyme in an optimized physical and chemical condition. Hence, from the present investigation, it is found that optimization of physic-chemical conditions may be a promising tool for the growth of organisms and also maximum yield of any metabolite.
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Askelson, Tyler E., Ashley Campasino, Jason T. Lee i Tri Duong. "Evaluation of Phytate-Degrading Lactobacillus Culture Administration to Broiler Chickens". Applied and Environmental Microbiology 80, nr 3 (22.11.2013): 943–50. http://dx.doi.org/10.1128/aem.03155-13.
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ABSTRACTProbiotics have been demonstrated to promote growth, stimulate immune responses, and improve food safety of poultry. While widely used, their effectiveness is mixed, and the mechanisms through which they contribute to poultry production are not well understood. Microbial phytases are increasingly supplemented in feed to improve digestibility and reduce antinutritive effects of phytate. The microbial origin of these exogenous enzymes suggests a potentially important mechanism of probiotic functionality. We investigated phytate degradation as a novel probiotic mechanism using recombinantLactobacilluscultures expressingBacillus subtilisphytase.B. subtilis phyAwas codon optimized for expression inLactobacillusand cloned into the expression vector pTRK882. The resulting plasmid, pTD003, was transformed intoLactobacillus acidophilus,Lactobacillus gallinarum, andLactobacillus gasseri. SDS-PAGE revealed a protein in the culture supernatants ofLactobacilluspTD003 transformants with a molecular weight similar to that of theB. subtilisphytase. Expression ofB. subtilisphytase increased phytate degradation ofL. acidophilus,L. gasseri, andL. gallinarumapproximately 4-, 10-, and 18-fold over the background activity of empty-vector transformants, respectively. Phytase-expressingL. gallinarumandL. gasseriwere administered to broiler chicks fed a phosphorus-deficient diet. Phytase-expressingL. gasseriimproved weight gain of broiler chickens to a level comparable to that for chickens fed a control diet adequate in phosphorus, demonstrating proof of principle that administration of phytate-degrading probiotic cultures can improve performance of livestock animals. This will inform future studies investigating whether probiotic cultures are able to provide both the performance benefits of feed enzymes and the animal health and food safety benefits traditionally associated with probiotics.
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Zhang, M., S. Chen, X. Chen, Y. Huang, L. Wei, B. Chen, Y. Wu i in. "238 PRODUCTION OF CLEAVAGE-RESISTANT PHYTASE TRANSGENIC PIGS BY HANDMADE CLONING". Reproduction, Fertility and Development 28, nr 2 (2016): 251. http://dx.doi.org/10.1071/rdv28n2ab238.
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Rapidly developing and intensive animal husbandry of livestock is a major contributor to global environmental pollutions. Large quantities and high concentrations of manure waste that contains phytate phosphorus are generated. The use of phytase can effectively solve the problem of high phosphorus pollution in the fecal material of monogastric animals. Enviropigs, producing phytase in the salivary glands and secreting the enzyme in the saliva, were first generated at the University of Guelph (Guelph, ON, Canada) in 1999. However, phytase is easily inactivated in digestive processing. To address this problem, we improved the transgene construct and successfully generated phytase transgenic pigs by handmade cloning. The Escherichia coli periplasmic phosphoanhydride phosphohydrolase (appA) gene was subcloned. Using fragment substitution method, we designed a phytase gene that was insensitive to cleavage by pepsin and trypsin and had a higher affinity for the substrate. After codon optimization, the designed phytase gene was named Cafp and subcloned downstream of the pig parotid secretory protein (PSP) gene promoter. The tissue-specific vector p-PSP-Intron-Cafp was constructed and transferred into Landrace fetal fibroblasts by electroporation. The cell lines carrying Cafp were used as nuclear donors in handmade cloning. Cloned embryos were cultured in vitro to blastocysts and transferred to recipient sows. The presence of Cafp was tested by PCR and sequencing of cloned pigs. Phytase activity in saliva, feed, and feces was detected by the ammonium molybdate method with a slight modification. Immunohistochemistry (IHC) was used to determine tissue-specific expression. Three cell lines carrying Cafp were obtained. We generated 1027 blastocysts; 712 were of good quality and transferred to 6 recipients. Fourteen piglets were born, of which 6 survived. The PCR and sequencing results showed that 7 (3 live and 4 dead) of the 14 piglets carried Cafp. Phytase activity in the saliva of the 6 live cloned pigs was tested at 4 months of age and only 1 pig had 0.155 FTU mL–1 enzyme activity. The enzyme in the other 2 pigs may be inactivated in the transgenic parotid gland. Among all the transgenic pigs, the highest phosphorus digestion rate was 59.2% of intake, which represents a 25.4% decrease in fecal emissions compared with the average of controls. The IHC results on the 3 later dead, Cafp-positive pigs showed that the transgene was expressed only in parotids, confirming tissue-specific gene expression. In summary, cleavage-resistant phytase transgenic pigs were successfully produced through handmade cloning. The cloned pigs offer a unique biological approach to manage phosphorus nutrition and environmental pollution in animal husbandry.
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Gauchan, Dhurva Prasad, Shishir Pandey, Bikash Pokhrel, Nabin Bogati, Puja Thapa, Ashesh Acharya, Bishnu Maya KC i Janardan Lamichhane. "Growth Promoting Role of Phytase Producing Bacteria Isolated from Bambusa tulda Roxb. Rhizosphere in Maize Seedlings Under Pot Conditions". Journal of Nepal Biotechnology Association 4, nr 1 (22.03.2023): 17–26. http://dx.doi.org/10.3126/jnba.v4i1.53442.
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Phytase enzymes have focused on their role in agriculture to generate bioavailable phosphorus (P) requires for plant nutrition. Herein, the feasibility of phytase-producing microbes as biofertilizers was studied. Bacteria with greater potential for hydrolysing calcium phytate based on the halo-to-colony ratio from Bambusa tulda Roxb. rhizosphere was isolated. Phytase activity by incorporating wheat bran, phytase screening and Luria Bertani (LB) medium after acetone precipitation was measured. Bacterial genomes were screened for the presence of β-propeller phytase gene corresponding to the Bacillus spp. using polymerase chain reaction (PCR). Finally, the effect of the isolates on the growth of maize seedlings under pot conditions in P-deficient loamy soil was evaluated. Ten distinct bacterial isolates collected from B. tulda rhizosphere were capable of mineralizing phytate and the maximum effect was observed for designated SRBR-04. Most isolates solubilized Ca3(PO4)2 as a sole P source in Pikovskaya’s agar. Five isolates selected for the study synthesized auxin in the LB broth supplemented with 1 mg mL-1 L-Tryptophan (1.63 to 4.5 μg mL-1). Phytase production was highest in wheat bran with isolate SRBR-04 producing a maximum of 0.34 U mg-1. Two isolates (SRBR-01, SRBR-04) screened positive for the presence of Bacillus phytase gene. Pot assay in P-deficient soil showed significant (p < 0.05) biomass promotion for the isolate SRBR-07 in shoot height (57%), dry shoot weight (178%), dry root weight (104%) and leaf area (113%) over the untreated control. Amendment of P-deficient agricultural soils with phytase-producing bacteria would provide a sustainable approach for P nutrition management in Zea mays.
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Tang, Hooi Chia, Chin Chin Sieo, Norhani Abdullah, Chun Wie Chong i Yin Wan Ho. "Preservation of phytase enzyme produced by anaerobic rumen bacteria, Mitsuokella jalaludinii". Journal of Biochemistry, Microbiology and Biotechnology 5, nr 1 (31.07.2017): 13–17. http://dx.doi.org/10.54987/jobimb.v5i1.334.
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Poultry feed consists of feed ingredients like soybean meal and corn, which contain high levels of phytate that is poorly utilised especially by the monogastric animals that lack of phytase. Hence, phytase has been extensively applied as a feed supplement in poultry production due to the efficiency of this enzyme in improving phosphorous (P) availability, thus reducing P excretion to the environment as well as reducing the feed cost by reducing inorganic P supplementation. Mitsuokella jalaludinii, an obligate anaerobe, Gram-negative rumen bacterium, produces high phytase activity. Birds supplemented with bacterial preparation of M. jalaludinii showed comparable performance to that of commercial phytase. However, the anaerobic nature of this bacterium renders difficulty in the use of live cells as feed supplement in commercial poultry production. Therefore, this study was conducted to determine a suitable method to preserve phytase activity of M. jalaludinii regardless of cells viability. Mitsuokella jalaludinii was grown in MF medium under anaerobic condition and the cells were subjected to various treatments to preserve the enzyme, including bead beating, compressed air, moist heat, dry heat and freeze-drying under aerobic condition. The results showed that the total number of viable cells were significantly (p<0.05) reduced when the cells were subjected to bead beating, whereas no viable cells were detected for compressed air, moist heat, dry heat and freeze-drying. Bead beating, compressed air, moist heat and dry heat treatments resulted in the reduction of phytase activity. However, only freeze-drying method was able to preserve high level of phytase activity significantly (p<0.05).
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Khan, Naheeda, i D. J. A. Cole. "Phosphorus equivalence of aspergillus niger phytase for growing pigs". Proceedings of the British Society of Animal Science 1995 (marzec 1995): 40. http://dx.doi.org/10.1017/s1752756200590383.
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Inconsistencies in the estimated phosphorus requirements of growing pigs have come to light in recent years due to environmental constraints being imposed on the livestock production sector. Values of a net requirement estimated by the ARC (1981) extend from 4.6 g/day for 25kg liveweight to 5.2 g/day for 45kg pigs, but current literature suggesting that the actual requirement may be lower (eg Jongbloed and Everts, 1991) calls for a revision of previous recommendations.Around two thirds of the total phosphorus in cereals exists in the form of insoluble phytates which must be degraded before the phosphorus can be absorbed in the intestine. Under experimental conditions, the use of phytase in pig diets has demonstrated consistent improvements in phosphorus digestibility. The enzyme hydrolyses phytate by stepwise removal of orthophosphates, which become available for absorption in the gut. Although quantification of phytase/phosphorus substitution is as yet undetermined, digestibility results so far indicate that if used correctly the enzyme could largely replace inorganic phosphates, particularly in regions where soil phosphate levels are of concern.
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Khan, Naheeda, i D. J. A. Cole. "Phosphorus equivalence ofaspergillus nigerphytase for growing pigs". Proceedings of the British Society of Animal Science 1995 (marzec 1995): 40. http://dx.doi.org/10.1017/s0308229600028099.
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Inconsistencies in the estimated phosphorus requirements of growing pigs have come to light in recent years due to environmental constraints being imposed on the livestock production sector. Values of a net requirement estimated by the ARC (1981) extend from 4.6 g/day for 25kg liveweight to 5.2 g/day for 45kg pigs, but current literature suggesting that the actual requirement may be lower (eg Jongbloed and Everts, 1991) calls for a revision of previous recommendations.Around two thirds of the total phosphorus in cereals exists in the form of insoluble phytates which must be degraded before the phosphorus can be absorbed in the intestine. Under experimental conditions, the use of phytase in pig diets has demonstrated consistent improvements in phosphorus digestibility. The enzyme hydrolyses phytate by stepwise removal of orthophosphates, which become available for absorption in the gut. Although quantification of phytase/phosphorus substitution is as yet undetermined, digestibility results so far indicate that if used correctly the enzyme could largely replace inorganic phosphates, particularly in regions where soil phosphate levels are of concern.
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Cangussu, Alex Sander Rodrigues, Deborah Aires Almeida, Raimundo Wagner de Souza Aguiar, Sidnei Emilio Bordignon-Junior, Kelvinson Fernandes Viana, Luiz Carlos Bertucci Barbosa, Edson Wagner da Silva Cangussu i in. "Characterization of the Catalytic Structure of Plant Phytase, Protein Tyrosine Phosphatase-Like Phytase, and Histidine Acid Phytases and Their Biotechnological Applications". Enzyme Research 2018 (11.03.2018): 1–12. http://dx.doi.org/10.1155/2018/8240698.
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Phytase plays a prominent role in monogastric animal nutrition due to its ability to improve phytic acid digestion in the gastrointestinal tract, releasing phosphorus and other micronutrients that are important for animal development. Moreover, phytase decreases the amounts of phytic acid and phosphate excreted in feces. Bioinformatics approaches can contribute to the understanding of the catalytic structure of phytase. Analysis of the catalytic structure can reveal enzymatic stability and the polarization and hydrophobicity of amino acids. One important aspect of this type of analysis is the estimation of the number of β-sheets and α-helices in the enzymatic structure. Fermentative processes or genetic engineering methods are employed for phytase production in transgenic plants or microorganisms. To this end, phytase genes are inserted in transgenic crops to improve the bioavailability of phosphorus. This promising technology aims to improve agricultural efficiency and productivity. Thus, the aim of this review is to present the characterization of the catalytic structure of plant and microbial phytases, phytase genes used in transgenic plants and microorganisms, and their biotechnological applications in animal nutrition, which do not impact negatively on environmental degradation.
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Duraid Thonnon Younis, Khalid Hadi Mustafa Al- Sofee, Duraid Thonnon Younis, Khalid Hadi Mustafa Al Sofee. "Effect replacement of soybean meal with the sesame meal as a protein source and adding of phytase enzyme to the diet on production performance and some physiological blood indicators of quail bird in the growth stage: تأثير استبدال كسبة فول الصويا بكسبة السمسم كمصدر بروتيني وإضافة انزيم الفايتيز إلى العليقة في الأداء الإنتاجي وبعض مؤشرات الدم الفسلجية لطائر السمان في مرحلة النمو". Journal of agricultural, environmental and veterinary sciences 5, nr 3 (30.09.2021): 57–43. http://dx.doi.org/10.26389/ajsrp.k240521.
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The study was conducted in the field of poultry in the Animal Production Department/ College of Agriculture and Forestry/ University of Mosul, aimed to study the effect of replacing soybean meal(SBM) with sesame meal(SSM) as a protein source and adding the phytase enzyme to the diet on productive performance and some physiological blood indicators of quail birds in the growth stage. five hundred quail birds were used at the age of one week unsexed and distributed randomly into ten treatments, each treatment was five replicates, ten birds for each replicate, birds reared until 42 days old. The experimental treatments were replacement of SBM with SSM in (0, 25, 50, 75 and 100%) without or with the addition of phytase (300 unit phytase/ kg diet). The results showed that there was no significant effect of replacement the sesame meal at rates that reached (75%) without or with the addition of phytase in the final live weight and weight gain as compared to the negative control (0% SSM without phytase). Significant improvement (P≤0.05) in feed conversion ratio for the two replacement treatments (50 and 75% SSM without phytase) compared to the negative control and a significant improvement for the replacement treatments (25, 50 and 75% SSM with addition of phytase) compared to the negative and positive controls. And there was a significant improvement in the production index in favor of the two replacement treatments (50 and 75% SSM with addition of phytase) as compared to the negative control only. The results showed a significant deterioration when replacing the sesame meal instead of soybean meal by (100%) without or with the addition of phytases in live body weight, weight gain, relative growth rate and production Index as well as a decrease in the percentage abdominal fat compared to the negative and positive controls and a decrease in %PCV as compared to only negative control. The replacement treatment (100% SSM without phytase) recorded a significant increase in dressing percentage as compared to the two controls. The results of this study showed the possibility of replacing soybean meal with sesame meal by up to 75% without or with the addition of phytase enzyme to the diets of quail in the growth stage without any negative effects on production performance.
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Sadaf, Naila, Muhammad Zulqurnain Haider, Naeem Iqbal, Muyassar H. Abualreesh i Aishah Alatawi. "Harnessing the Phytase Production Potential of Soil-Borne Fungi from Wastewater Irrigated Fields Based on Eco-Cultural Optimization under Shake Flask Method". Agriculture 12, nr 1 (12.01.2022): 103. http://dx.doi.org/10.3390/agriculture12010103.
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Indigenous fungi present in agricultural soils could have synchronized their inherent potentials to the local climatic conditions. Therefore, the fungi resident in the untreated wastewater irrigated agricultural field might develop their potential for producing various enzymes to handle the induced full organic load from domestic wastewater and toxic chemicals from the textile industry. Around 53 various fungal isolates were grown and separated from the soil samples from these sites through soil dilution, soil-culture plate, and soil-culture plate methods. All the purified fungi were subjected to a phosphatase production test, and only 13 fungal strains were selected as phosphatase producers. Among them, only five fungi identified as Aspergillus niger, Aspergillus flavus, Aspergillus fumigatus, Penicillium purourogenum, and Mucor rouxii based on morphological similarities, showing higher phosphate solubilizing indices, were utilized for eco-cultural fine-tuning to harness their full production potential under shake flask (SF) method. Among various media, orchestral tuning, 200 µM sodium phytate as substrate with 1.5 mL of inoculum size of the fungi, pH 7, temperature 30 °C, glucose, and ammonium nitrate as carbon and nitrogen additive with seven days of incubation were found to be the most appropriate cultural conditions to harness the phytase production potential of the selected fungi. Aspergillus niger and Aspergillus flavus showed initial phytase activity (5.2 Units/mL, 4.8 Units/mL) and phytase specific activity (2.85, 2.65 Units/mL per mg protein) during screening to be enhanced up to 17 ± 0.033 (Units/mL), 16 ± 0.033 (Units/mL) and (13 ± 0.012), 10 ± 0.066 (Units/mL per mg protein), respectively, with the above-mentioned conditions. The phytase enzyme produced from these fungi were found to be almost stable for a wide range of pH (4–8); temperature (20–60 °C); insensitive to Ca2+ and Mg2+ ions, and EDTA, Ni2+, and Ba2+ inhibitors but highly sensitive to Mn2+, Cu2+, and Zn2+ ions, and Co2+, Cr3+, Al3+, Fe2+ and Ag1+ inhibitors. It was suggested that both phytase-producing strains of A. niger and A. flavus or their crude phytase enzymes might be good candidates for application in soils to release phosphates from phytate and a possible valuable substitute of phosphate fertilizers.
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Kolokolnikov, N., E. Amiranashvili, A. Yatsishin, E. Chaunina, I. Mezentsev i M. Mezentsev. "Super dose of phytase in compound feed for turkey poults". Kormlenie sel'skohozjajstvennyh zhivotnyh i kormoproizvodstvo (Feeding of agricultural animals and feed production), nr 9 (1.09.2020): 12–19. http://dx.doi.org/10.33920/sel-05-2009-02.
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In the practice of feeding poultry enzyme drugs are widely used that contribute to the effective transformation of feed components into poultry products. Reducing the cost of compound feed for poultry due to the introduction of enzyme drugs is of great interest, both from a scientific and practical point of view. More than 2/3 of phosphorus in plant feeds is represented in the form of phytate and in this form the element is only partially absorbed in the poultry body. Accordingly to cover the need for phosphorus in plant diets include inorganic phosphates, animal feed and the enzyme phytase (breaks down phytate). This makes phosphorus the third most expensive feed component after energy and protein. The purpose of the researches was to study the effectiveness of using super dose commercial phytase in feeding of turkey poults. The results of research on the use of high doses of phytase in the diet of turkey poults of cross Hybrid Converter have been presented. It has been found that the use of compound feeds containing the super dose of phytase in the rearing of broiler turkey poults does not have a negative influence on the zootechnical indicators of poultry rearing, meat productivity, and increases the economic indicators of meat production. The results on base the experiment, economic indicators have been calculated. It has been found that the cost of 1 ton of compound feed consumed in the experimental group was less than in the control group by 166,28 rubles or 0,7 %. The use of high doses of phytase in the diet of turkey poults of the experimental group allowed to reduce the cost of growth of 1 kg of live weight by 4,7 % and increase the profitability of meat production.
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Zailan, Nur Dinie, Nurul Asma Hasliza Zulkifly, Afnani Alwi, Siti Noor Syuhada Muhammad Amin i Nadiawati Alias. "Effects of Nitrogen Sources in Phytase Production on Bacterial Strains Isolated from Malaysia’s Hot Spring". Journal Of Agrobiotechnology 12, nr 1S (29.09.2021): 31–39. http://dx.doi.org/10.37231/jab.2021.12.1s.268.
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Efficient strategies for phytase production gained increasing importance as more applications require high amounts of phytase for the market. Four phytase-producing bacterial strains isolated from Malaysia’s hot springs were used in this study to determine the effect of nitrogen sources on phytase production. All of the strains were screened out by applying halozone method which shows all of the strains were definitely positive phytase producer. Phytase Screening Medium (PSM) with soybean extract as substrate was used as a cultivation medium. Optimised condition with 1.0 % (w/v) of glucose (as carbon source), pH 5.5 and 37°C temperature was applied. Yeast extract and peptone were used to identify optimum nitrogen source in maximum phytase production. Quantitative analysis observed were optical density, colony forming unit, pH values and phytase activity to identify the effect of nitrogen source in phytase production. The finding was bacterial strain L3 as the best producer in producing maximum phytase (0.2162 U/mL) with optimised condition using yeast extract as nitrogen source. Findings in this study proved that yeast extract act as the optimum nitrogen source which contribute to maximum phytase production as supported by previous studies. This study can provide an efficient strategy to produce maximum phytase as few studies stated that phytase is an application tool in functional food production that consists of myo-inositol phosphates that is believed to have important pharmacological effects.
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Ariff, Rafidah Mohd, Anwar Fitrianto, Mohd Yazid Abd Manap, Aini Ideris, Azhar Kassim, Afinah Suhairin i Anis Shobirin Meor Hussin. "Cultivation Conditions for Phytase Production from Recombinant Escherichia coli DH5α". Microbiology Insights 6 (styczeń 2013): MBI.S10402. http://dx.doi.org/10.4137/mbi.s10402.
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Response surface methodology (RSM) was used to optimize the cultivation conditions for the production of phytase by recombinant Escherichia coli DH5α. The optimum predicted cultivation conditions for phytase production were at 3 hours seed age, a 2.5% inoculum level, an L-arabinose concentration of 0.20%, a cell concentration of 0.3 (as measured at 600 nm) and 17 hours post-induction time with a predicted phytase activity of 4194.45 U/mL. The model was validated and the results showed no significant difference between the experimental and the predicted phytase activity ( P = 0.305). Under optimum cultivation conditions, the phytase activity of the recombinant E. coli DH5α was 364 times higher compared to the phytase activity of the wild-type producer, Enterobacter sakazakii ASUIA279. Hence, optimization of the cultivation conditions using RSM positively increased phytase production from recombinant E. coli DH5α.
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Pirgozliev, V., i M. R. Bedford. "Energy utilisation and growth performance of chicken fed diets containing graded levels of supplementary bacterial phytase". British Journal of Nutrition 109, nr 2 (13.04.2012): 248–53. http://dx.doi.org/10.1017/s0007114512000943.
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A total of 364 female Ross 308 chicks (1 d old) were used in the present study conducted in floor pens to investigate the effects of graded levels of supplementary bacterial phytase on dietary energy utilisation and growth performance. For this purpose, four maize–soyabean-based diets were offered to the birds from 0 to 21 d of age. These included a suboptimal P negative control (NC, 3·0 g/kg non-phytate P), NC+250 phytase units (FTU)/kg feed, NC+500 FTU and NC+2500 FTU. The effect of phytase activity on bird growth performance was best described as a linear relationship between increasing dose and increased feed intake (P< 0·001), but was quadratic for body-weight gain (P= 0·002) and feed efficiency (P= 0·023). There was no significant response (P>0·05) of dietary apparent metabolisable energy (AME) to supplementary phytase. The birds fed phytase increased their retention of total carcass energy in a linear fashion (P= 0·009) with increased phytase dose. The efficiency of dietary AME used for overall carcass energy retention also improved (P= 0·007) in a linear manner with increased dietary phytase activity. Dietary net energy for production (NEp) increased (P= 0·047) with an increase in phytase dose following a linear pattern, as an increase of 100 FTU increased dietary net energy by 15·4 J (estimated within the range of doses used in the present experiment). Dietary NEp was more highly correlated with performance criteria than dietary AME, and it seems to be a more sensitive way to evaluate broiler response to phytase supplementation.
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Itkina, Daria L., Aliya D. Suleymanova i Margarita R. Sharipova. "Expression of Bacillus ginsengihumi M2.11 bacterial phytase by recombinant Pichia pastoris strains". BIO Web of Conferences 36 (2021): 07013. http://dx.doi.org/10.1051/bioconf/20213607013.
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Phytic acid is the main storage form of organic phosphorus. Due to its structural features, phosphorus in phytate is inaccessible for assimilation by animals. Moreover, remaining inaccessible reservoir of phosphorus for animal nutrition, phytic acid is capable of forming insoluble complex salts, which lead to soil and water pollution. Мicrobial enzymes - phytases, capable of decomposing phytic acid to organic phosphorus are being used as feed additives in animal nutrition to solve this problem. Thus, search and development of technologies for the production of enzymes on an industrial scale are the most urgent. Methylotrophic yeast P. pastoris are widely used in biotechnology, as an efficient system for the recombinant proteins expression. They have many advantages, including rapid growth on inexpensive media, a wide range of molecular tools for genetic manipulation in optimizing production processes, they are safe for humans and animals, carry-out many post-translational modifications and produce recombinant proteins intracellularly or extracellularly within a short period of time. It was found that the recombinant P. pastoris strains pPINK-LC-α-MF -phyC, pPINK-HC-α-amyl -phyC, pPINK-LC-α-amyl -phyC, pPINK-HC-α-MF -phyC are able to produce and to secrete B. ginsengihumi bacterial phytase M 2.11 phyC. The maximum activity was observed in the pPINK-LC-α-MF strain – 2.6 (U / mg). Recombinant B. ginsengihumi M 2.11 phytases exhibited high activity in a wide pH range from 2.5 to 9.0. The MF-phyC-HC construction is pH stable. The temperature optimum of all recombinant phytases corresponds to 37 ° C; recombinant phytases retain their activity in the range from -80 to 90C.
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Lucky, NJ, MAR Howlider, MA Alam i MF Ahmed. "Effect of dietary exogenous phytase on laying performance of chicken at older ages". Bangladesh Journal of Animal Science 43, nr 1 (30.06.2014): 52–55. http://dx.doi.org/10.3329/bjas.v43i1.19385.
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A total of 48 Shaver-579 chicken layers aged between 85 to 94 weeks were reared in individual cages and given a basal diet amounting to 115g feed/bird/day. The basal diet fortified with 0.05, 0.10 or 0.15% Rena-Phytase-400 constituted of 3 experimental diets to see the effects of exogenous phytase on egg production and egg quality. Results indicated that increasing level of exogenous phytase in diet almost linearly (p<0.05) increased egg production and feed conversion but did not affect egg quality. Providing phytase in the diet at 0.05, 0.10 and 0.15% increased egg production by 11.86, 22.2 and 24.58%, respectively. It was shown that highest egg production was found at 0.15% phytase levels in diet. It can be concluded that egg production of aged hen could be increased by adding said amount of exogenous phytase in the diet.DOI: http://dx.doi.org/10.3329/bjas.v43i1.19385 Bang. J. Anim. Sci. 2014. 43 (1): 52-55
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McCann, M. E. E., K. J. McCracken, V. E. Beattie, W. Henry, S. Smyth i R. Bradford. "The use of phytase in finishing pig diets". Proceedings of the British Society of Animal Science 2003 (2003): 66. http://dx.doi.org/10.1017/s1752756200012254.
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Phosphorus (P) is an essential mineral for pigs and deficiency can cause rickets, osteomalacia or osteoporosis and has been associated with poor fertility and production performance. Traditionally P has been supplied in the inorganic form as the organic form found in cereal grain (phytic acid) is unavailable to pigs as they lack endogenous phytase. Dietary phytases have been shown to play a major role in pig diets, releasing P from phytic acid thus decreasing the need for supplementary P and reducing P excretion (Harper et al 1997). Phytase supplementation has also been reported to improve digestibility of nitrogen, calcium, magnesium, zinc and copper (Jongbloed et al 1993). Some researchers (e.g. Jongbloed and Kemme 1990) suggest that diet processing may reduce enzyme activity and therefore liquid enzyme products have been developed which can be applied after processing. The aims of this study were to examine the effectiveness of several phytase products (including dry vs. liquid) in improving overall digestibility and reducing excretion of P and other nutrients.
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Ekren, Gamze Sevrı, i Kubilay Metin. "Production, purification and characterization of phytase from phytase producing fungus". Current Opinion in Biotechnology 24 (lipiec 2013): S81. http://dx.doi.org/10.1016/j.copbio.2013.05.234.
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Parvin, E., MA Khatun, T. Yeasmin i MM Sharmin. "Efficacy of exogenous phytase supplementation on production performance of commercial broiler". Bangladesh Journal of Animal Science 45, nr 3 (9.01.2017): 31–39. http://dx.doi.org/10.3329/bjas.v45i3.31037.
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An experiment was conducted to investigate the effect of supplementing of exogenous phytase enzyme on productivity of commercial broilers. Four strains of commercial broiler such as Fast feather, Arbor acres, Cobb-500 and Hubbard classic each having 78 numbers were reared for a period of 5 weeks. A total number of 312 day old broiler chicks were distributed into two dietary treatments i.e. basal diet (Control-T0) and basal diet supplemented with phytase enzyme 1g/kg feed (supplemented-T1). The results indicated that broilers fed on diets supplemented with phytase have gained more body weight when compared with control group (P<0.05). The first and second highest body weights were recorded for the strains Cobb-500 and First feather broilers, respectively, which feed on diet containing phytase. Feed consumption varied significantly between the broilers on different dietary regimes. Significant differences (P<0.01) were found in feed conversion ratios among the birds fed on diet with phytase enzyme. The lowest feed conversion ratio was in Cobb-500 fed on phytase diet where as the highest was in Fast Feather provided with only basal diet during the 5th week of age. Average feed conversion ratios were 1.73 and 1.86 for the supplemented and control group respectively. Use of phytase as feed additive in the diet resulted in high profitability/ broiler. Net profit per live broiler was the also higher in treatment group compared with control groups of birds. The highest net profit per live broiler was observed in Arbor Across strains fed on phytase diet. The result of the present study suggests that the addition of dietary phytase enzyme in the diet of broiler may increase the production performance and reduce the cost of production.Bang. J. Anim. Sci. 2016. 45 (3): 31-39
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Zaworska-Zakrzewska, Anita, Małgorzata Kasprowicz-Potocka, Bartosz Kierończyk i Damian Józefiak. "The Effect of Solid-State Fermentation on the Nutritive Value of Rapeseed Cakes and Performance of Broiler Chickens". Fermentation 9, nr 5 (30.04.2023): 435. http://dx.doi.org/10.3390/fermentation9050435.
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This study aimed to assess the applicability of eleven different products of solid-state fermentation of rapeseed cakes with commercial enzyme additives. Ground rapeseed cakes were mixed with water (1:2) and the enzymatic preparation (0.1%) according to the different variants and fermented at 25 °C for 24 h under anaerobic conditions. For fermentation, different enzymes were used: (1) α-amylase, (2) endo 1,4-β-xylanase, (3) endo-1,3(4)β-glucanase, (4) endo 1,4-β-xylanase, endo-1,3(4)β-glucanase, (5) α-amylase, endo-1,3(4)β-glucanase, (6) 6-phytase, (7) RONOZYME HiPhos 6-phytase, (8) liquid exogenous-6-phytase; and mixed combinations: (9) endo 1,4-β-xylanase, endo-1,3(4)β-glucanase, liquid exogenous-6-phytase, (10) α-amylase, endo-1,3(4)β-glucanase, liquid exogenous-6-phytase, and (11) α-amylase, endo 1,4-β-xylanase, endo-1,3(4)β-glucanase, liquid exogenous-6-phytase. After fermentation, the crude protein content in the products was similar, but the true protein content was significantly higher than in nonfermented rapeseed cakes (p < 0.05). Fermentation significantly reduced (p < 0.05) the levels of phytate phosphorous, raffinose family oligosaccharides, and glucosinolates in the products. In the next step, the most advantageous fermentation product obtained using liquid exogenous-6-phytase was selected in terms of nutritional value, produced on the technical scale, and incorporated into the diets for broilers. Six diets containing 12, 17, or 22% raw or fermented rapeseed cakes, respectively, were prepared. Up to 22% fermented rapeseed cakes did not adversely affect broiler production rates. Replacing raw rapeseed cakes with fermented products improved body weight gain in the grower phase, lowered feed intake in the finisher phase, and lowered the feed conversion ratio in all periods. A level of 22% of naturally fermented rapeseed cakes with liquid exogenous-6-phytase in the diet of broilers could be recommended.
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Batistela, Aline Maria Pereira, Marisangela Isabel Wietzikoski Halabura, Katielle Vieira Avelino, Kariny Aparecida Jardim Rubio, Daniela Dib Gonçalves, Giani Andrea Linde Colauto, Nelson Barros Colauto, Maria Graciela Iecher Faria i Juliana Silveira Valle. "Phytase production by white-rot fungi cultivated in liquid medium with particulate agro-industrial byproducts". Concilium 23, nr 5 (31.03.2023): 250–64. http://dx.doi.org/10.53660/clm-1075-23c74.
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This study aimed to screen phytase-producing basidiomycetes by submerged cultivation (SmC) with particulate agro-industrial byproducts and evaluate the enzyme's thermal and pH stability. Five among eight fungal species produced high phytase activity (> 300 U mL-1) after seven days in SmC. Ganoderma sp., the best phytase producer (1130 U mL-1), was grown in a liquid medium with particulate agro-industrial byproducts (coffee husks, citric pulp, sugarcane bagasse, and soybean hulls). Soybean hulls increased phytase activity (16%) and productivity (2.3-fold). The residual phytase activity was determined after 120 min incubation in different pH (pH 3, 5, 7, and 9) and temperatures (30, 50, and 70 oC). Phytase activity remained stable at pH ranging from 3 to 9, with 100% activity retention after 120 min at pH 7. Furthermore, Phytase retained more than 60% activity when incubated for 120 min from 30 oC to 70 oC. Our findings support future studies using Ganoderma sp. and particulate soybean hulls for phytase production for potential use in the animal feed industry.
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Hussin, Anis Shobirin Meor, Abd El Aziem Farouk i Ralf Greiner. "Optimization of cultivation conditions for the production of phytate-degrading enzymes by Enterobacter sakazakii ASUIA279 isolated from Malaysian maize root". Journal of Biotechnology and Biodiversity 3, nr 2 (16.05.2012): 1–10. http://dx.doi.org/10.20873/jbb.uft.cemaf.v3n2.hussin.
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The production of extracellular phytase by Enterobacter sakazakii ASUIA279 was optimized using response surface methodology with full-factorial faced centred central composite design. Two sets of experiments were carried out to optimize the five most profound factors of the cultivation conditions in order to maximize phytase production. Incubation temperature, initial pH of the media and percentage of rice bran supplemented into the media were optimized in Erlenmeyer flasks while agitation and aeration effect were controlled in a bioreactor. This design reduced the number of actual experiment performed to optimize phytase production and allowed the study of possible interactions among the factors. In the first set of experiments linear and quadratic effect of initial pH was determined to be the most significant factor affecting phytase production. In the bioreactor both linear effects of agitation and aeration, were identified to be highly significant (> 99 %) in respect to phytase yields. Optimal phytase production was observed at a incubation temperature of 39.7 ºC, an initial pH of 7.1, supplementation with 13.6 % rice bran , 320 rpm of agitation and 0 vvm of aeration.
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Musilova, A., M. Lichovnikova, L. Kupcikova i V. Anderle. "Effect of suboptimal levels of non-phytate phosphorus and exogenous phytase on precaecal digestibility of phosphorus and calcium in laying hens". Czech Journal of Animal Science 62, No. 11 (9.10.2017): 473–81. http://dx.doi.org/10.17221/16/2017-cjas.
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The precaecal digestibility of phosphorus and calcium in laying hens was evaluated at two dietary levels of non-phytate phosphorus (NPP; 1.58 and 2.03 g/kg) and two levels of exogenous 3-phytase (F; 0 and 300 phytase units (FTU)/kg). A total of 192 ISA Brown hens were used for the study, and were housed in enriched cages (8 per cage). At the end of the experiment, which was the peak of the egg production, the content of the posterior half of the ileum from each hen was collected. The lower level of NPP significantly (P < 0.05) improved the precaecal phosphorus digestibility coefficient. There was no significant difference in the amount of absorbed phosphorus between the lower and the higher level of NPP. The level of NPP with supplementation of exo-genous phytase had a significant effect (P < 0.001) on the concentration of phosphorus in ileum, while the higher level of NPP increased phosphorus concentration in the ileum and phytase decreased phosphorus content in the ileal digesta. The phytase had no significant effect on phosphorus precaecal digestibility. There was no significant effect of the both factors, level of NPP, and exogenous phytase on the daily calcium intake, the amount of absorbed calcium, the content of calcium in ileal digesta, and on the precaecal digestibility of calcium. However a significant effect (P < 0.05) of the interaction between NPP and phytase was observed on the amount of absorbed calcium and on the precaecal digestibility coefficient. It can be concluded that the estimation of phosphorus ileal digestibility depends on the level of dietary phosphorus.
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Jafari-Tapeh, H., Z. Hamidi-Esfahani i M. H. Azizi. "Culture Condition Improvement for Phytase Production in Solid State Fermentation by Aspergillus ficuum Using Statistical Method". ISRN Chemical Engineering 2012 (27.11.2012): 1–5. http://dx.doi.org/10.5402/2012/479167.
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The effective factors on phytase production by Aspergillus ficuum PTCC 5288 were studied using solid-state fermentation method in 250 ml shake flask. The effective process parameters on phytase production were identified using Plackett-Burman design. Four factors were identified of different variables, including glucose, moisture, MgSO4, and fermentation time, which were the most significant. The optimum levels of these significant parameters were determined through response surface methodological approach as follows: 10.14% glucose, 62.69% moisture, 0.46% MgSO4 and 119.23 h. The maximum predicted amount of phytase was 24.33 U/gds and the produced amount of phytase under these conditions was 25.6 U/gds, which indicates the efficacy of the model for prediction of phytase production content under different medium conditions.
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Nascimento, Júlio Cézar dos Santos, Apolônio Gomes Ribeiro, Ricardo Alexandre Silva Pessoa, Carlos Bôa Viagem Rabello, Armando Venâncio, Tatiana Souza Porto, José António Couto Teixeira i Ana Lúcia Figueiredo Porto. "Effect of pH and temperature on phytase and biomass production by submerged fermentation with Aspergillus niger var. phoenicis URM 4924". Research, Society and Development 11, nr 6 (13.05.2022): e41311628994. http://dx.doi.org/10.33448/rsd-v11i6.28994.
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The production of phytase and biomass (estimated by the ergosterol content) by submerged fermentation with Aspergillus niger var. phoenicis URM 4924 was studied. Experimental assays were carried under different conditions of pH (4.0 to 8.0) and temperature (25 to 35 ºC), and the influence of these variables on the responses was studied through a 22 central composite design and response surface methodology. Phytase and biomass production were strongly affected by the pH and temperature used during fermentation. Phytase activity was increased in up to 7.8-fold (from 1.04 to 8.09 U/mL) and the ergosterol content was increased in up to 38-fold (from 9.3 to 354.09 µg/mL). The maximum values of both responses were achieved when using pH 4.0 and 30 ºC. Good correlation (second-order fit, R2 = 0.9875) was found between the data obtained for phytase activity and ergosterol content, suggesting that the phytase production depends on the biomass formation. These results are of interest since they contribute for the development of an industrial process for phytase production with elevated yields by submerged fermentation.
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Faria, Henrique Gonsales de, Maria Cristina Thomaz, Urbano Dos Santos Ruiz, Rizal Alcides Robles-Huaynate, Pedro Henrique Watanabe, Gabriel Maurício Peruca de Melo i Susana Zaneti da Silva. "Effects of phytase on pig diets digestibilities, bone mineral deposition, performance and manure production". Semina: Ciências Agrárias 36, nr 6Supl2 (16.12.2015): 4519. http://dx.doi.org/10.5433/1679-0359.2015v36n6sup2p4519.
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Two trials were conducted to evaluate phytase addition to diets with corn, rice and soybean meals for swine. In the first trial, the digestibilities of gross energy and crude protein and the availabilities of calcium (Ca) and phosphorous (P) of diets with or without phytase were evaluated in growing barrows (BW 20.42 ± 2.45 kg), using the total feces and feed collection methods. In the second trial, the parameters evaluated were pig performance, mineral contents in the 3rd and 4th metacarpals, feces production and composition of growing and finishing gilts fed one of the following diets: Positive control (PC), formulated to achieve nutrient and energy requirements of growing and finishing gilts; Negative control (NC), diet PC meeting 84% and 65% of Ca and P levels, respectively; Negative control + phytase (PHY), diet NC + 0.01% phytase (500 FTU/kg). Phytase addition improved P and Ca availabilities in 14.34% and 4.08%, respectively, compared with the diet with no phytase, in trial one. In trial two, the deposition of zinc in the metacarpals was improved by the enzyme and Ca, P and copper fecal excretions of the animals from the PC diet were greater than that observed for the PHY animals. Phytase addition improved the use of phytic phosphorous and calcium by the pigs and is recommended as a way to reduce the potential environmental impact of swine production.
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Farouk, Abd-El Aziem, Anis Shobirin Meor Hussin, Ralf Greiner, Shareef Mohideen Ismail i Hamadah Mohd Nur Lubis. "Statistical Optimization of the Induction of Phytase Production by Arabinose in a recombinant E. coli using Response Surface Methodology". ASEAN Journal on Science and Technology for Development 26, nr 1 (26.11.2017): 57–64. http://dx.doi.org/10.29037/ajstd.304.
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The production of phytase in a recombinant E.coli using the pBAD expression system was optimized using response surface methodology with full-factorial faced centered central composite design. The ampicilin and arabinose concentration in the cultivation media and the incubation temperature were optimized in order to maximize phytase production using 2 3 central composite experimental design. With this design the number of actual experiment performed could be reduced while allowing eludidation of possible interactions among these factors. The most significant parameter was shown to be the linear and quadratic effect of the incubation temperature. Optimal conditions for phytase production were determined to be 100 µg/ml ampicilin, 0.2 % arabinose and an incubation temperature of 37ºC. The production of phytase in the recombinant E. coli was scaled up to 100 ml and 1000 ml.