Rozprawy doktorskie: „Phosphoproteins”

1

Marciniak, Stefan John. "Phosphoproteins of the zymogen granule membrane". Thesis, University of Cambridge, 1995. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.243154.

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2

Poulain, Fabienne. "Stathmin family phosphoproteins and neuronal morphogenesis". Paris 6, 2007. http://www.theses.fr/2007PA066492.

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Le développement des prolongements neuronaux requiert une réorganisation dynamique des microtubules. Les protéines de la famille de la stathmine (dont SCG10, SCLIP et RB3) participent au contrôle de la dynamique des microtubules en séquestrant la tubuline. Leur profil d’expression dans le système nerveux suggère qu’elles jouent des rôles distincts et complémentaires lors de la morphogenèse neuronale. Cette étude visait à mieux comprendre leur diversité fonctionnelle et a permis de caractériser les fonctions spécifiques de SCLIP dans l’axonogenèse et la dendritogenèse. Nous avons ainsi démontré que SCLIP régule la morphogenèse axonale des neurones d’hippocampe différemment d’SCG10, contrôlant le développement de nouvelles branches et non la surface du cône de croissance. Nous avons de plus révélé que SCLIP est fortement exprimée dans les cellules de Purkinje lors du développement du cervelet et joue un rôle primordial dans leur développement dendritique. Ces résultats ouvrent ainsi de nouvelles perspectives quant aux régulations fonctionnelles des protéines de la famille de la stathmine dans les neurones.

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3

Sheppard, Vonda Chantal. "Identification and characterization of diatom kinases catalyzing the phosphorylation of biomineral forming proteins". Diss., Georgia Institute of Technology, 2010. http://hdl.handle.net/1853/37227.

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Diatoms are unicellular photosynthetic algae that display intricately patterned cell walls made of amorphous silicon dioxide (silica). Long-chain polyamines and highly phosphorylated proteins, silaffins and silacidins, are believed to play an important role in biosilica formation. The phosphate moieties on silaffins and silacidins play a significant role in biomineral formation, yet no kinase has been identified that phosphorylates these biomineral forming proteins. This dissertation describes the characterization of a novel kinase from the diatom Thalassiosira pseudonana, tpSTK1, which is upregulated during silica formation. A recombinantly expressed histidine-tagged version of tpSTK1 was capable of phosphorylating recombinant silaffins but not recombinant silacidin in vitro. Through establishing methods for subcellular fraction of T. pseudonana membranes in combination with antibody inhibition assay, it was discovered that native tpSTK1 phosphorylates silaffins but not silacidins in vitro (i.e. it exhibits the same substrate specificity as recombinant tpSTK1). As tpSTK1 is an abundant protein in the ER lumen (~ 0.5 % of total ER protein) it seems highly likely to function as a silaffin kinase in vivo. TpSTK1 lacks clear sequence homologs in non-diatom organisms and is the first molecularly characterized kinase that appears to be involved in biomineralization. The predicted kinase domain (KD) of tpSTK2, the only T. pseudonana homolog of tpSTK1, was recombinantly expressed and tested for phosphorylation activity. Recombinant tpSTK2-KD and native tpSTK2 exhibited detectable activity with myelin basic protein, but did not phosphorylate silaffins or silacidins in vitro. Western blot analysis demonstrated that native tpSTK2 was not present in the ER, but associated with the cytosol and Golgi membrane containing subcellular fractions.

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4

Lei, Xia. "Identification and characterization of endosomal specific phosphoproteins". Thesis, McGill University, 1994. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=28483.

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Endosomal fractions isolated from rat liver homogenates harbor membrane-associated kinases that phosphorylate a 48-kDa extrinsic membrane protein and two integral membrane phosphoproteins of 35 and 31 kDa. These phosphoproteins were not detected in subcellular fractions enriched in plasma membranes, endoplasmic reticulum, lysosomes, coated vesicles and cytosol. Although the same phosphoproteins were also found in a mixed Golgi-endosome fraction (Gi), they were not found in endosome-free, highly purified Golgi fractions and were exclusively restricted to endosomes containing internalized horseradish peroxidase as determined by the diaminobenzidine shift protocol, therefore these phosphoproteins are unique to endosomes. Further characterization studies showed the 48-kDa protein was phosphotylated on threonine and the 35 and 31 kDa proteins were phosphorylated on serine residues. None of them were N-glycosylated. The endosomal protein kinase was independent of Ca$ sp{2+},$ cyclic nucleotide and phospholipids and was inhibited by N-ethylmaleimide (NEM). Both ATP and GTP were phosphate donors, and ATP and GTP phosphorylated identical endosomal phosphoproteins as determined by two-dimensional electrophoresis. Because the phosphorylation was not inhibited by heparin, the corresponding kinase may not be casein kinase II. We also observed that upon in vivo administration of saturating dose of EGF, the in vitro phosphorylation activity of the 48, 35, 31 kDa proteins decreased. The endosomal specific membrane phosphoproteins may mediate phosphorylation dependent processes in endosomes in addition to serving as novel markers for the organelle.

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5

Staubli, Justin Charles. "Development of a phosphoprotein enrichment method to identify and characterize phosphoproteins within leukemia following treatment with the PP2A activator, FTY720". The Ohio State University, 2012. http://rave.ohiolink.edu/etdc/view?acc_num=osu1338269265.

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6

Chen, Zhaoyuan. "Development of Methods for the Study of Phosphoproteins". Diss., CLICK HERE for online access, 2006. http://contentdm.lib.byu.edu/ETD/image/etd1629.pdf.

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7

Goswami, Suranjana. "IDENTIFICATION OF PHOSPHOPROTEINS INVOLVED IN SPERM MATURATION AND FERTILITY". Kent State University / OhioLINK, 2018. http://rave.ohiolink.edu/etdc/view?acc_num=kent1532952768427828.

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8

Sheehan, M. A. "Phosphoproteins in malignant and non-malignant human-human cell hybrids". Thesis, University of Oxford, 1985. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.355800.

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9

Rothman, Deborah Maria. "Caged phosphopeptides and phosphoproteins : agents in unraveling complex biological pathways". Thesis, Massachusetts Institute of Technology, 2005. http://hdl.handle.net/1721.1/32429.

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Thesis (Ph. D.)--Massachusetts Institute of Technology, Dept. of Chemistry, 2005.
Vita.
Includes bibliographical references.
Within cellular signaling, protein phosphorylation is the post-translational modification most frequently used to regulate protein activity. Protein kinases and phosphoprotein phosphatases generate and terminate these phosphoryl signals, respectively. Chemical approaches for studying protein phosphorylation and the roles of phosphoproteins include photolabile caged analogs of bioactive species. Caged compounds are ideal chemical probes for studying cellular signaling because they afford researchers spatial and temporal control over the release of targeted effector molecules. Ligands or proteins involved in signal transduction can be chemically caged and subsequently irradiated to release a concentration burst of a specific species, allowing the downstream effects to be monitored without disrupting other aspects of the system. The syntheses and applications of caged phosphopeptides and phosphoproteins have been developed and detailed within this thesis. Two methods to synthesize 1-(2-nitrophenyl)ethyl caged phosphopeptides were developed. These peptides demonstrated good quantum yields of uncaging as compared to literature values of other ortho-nitrobenzyl derived caged compounds. A study in which these caged phosphopeptide tools yielded seminal information about the 14-3-3 protein family's involvement in cell cycle control successfully demonstrated the unique utility of these probes. Furthermore, the synthesis that allowed the extension of the nonsense codon suppression methodology to include caged phosphoproteins was developed.
(cont.) The three most commonly phosphorylated amino acids (serine, threonine, and tyrosine) were each incorporated into a test protein in their caged phosphorylated form. Toward studying cell motility, caged phosphoserine was incorporated into position 153 of mVASP for use in live cell assays.
by Deborah Maria Rothman.
Ph.D.

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10

Neubauer, Gitte Jackie. "Microcharacterisation of multi-protein complexes and phosphoproteins by nanoelectrospray mass spectrometry". Thesis, University of London, 1997. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.299339.

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11

Zitzmann, Sabine. "In-situ-Analysen des exocytosesensitiven Phosphoproteins PP63/Parafusin in Paramecium-Zellen". [S.l.] : Universität Konstanz , Fakultät für Biologie, 1998. http://www.bsz-bw.de/cgi-bin/xvms.cgi?SWB8214570.

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12

Clifford, Gary. "The translocation of hormone-sensitive lipase and perilipin during lipolysis". Thesis, University of Newcastle Upon Tyne, 1999. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.285397.

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13

Zhang, Yun. "Activation of Erk1/2 and Akt in astrocytes under ischemia /". View Abstract or Full-Text, 2002. http://library.ust.hk/cgi/db/thesis.pl?BIOL%202002%20ZHANGY.

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Thesis (M. Phil.)--Hong Kong University of Science and Technology, 2002.
Includes bibliographical references (leaves 98-115). Also available in electronic version. Access restricted to campus users.

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14

Pekar, Tonya M. "Phosphoproteomic studies of smooth muscle contraction investigation of differential phosphorylation in relaxed/contracted rat aortic smooth muscle tissue using MALDI-TOF MS /". Huntington, WV : [Marshall University Libraries], 2003. http://www.marshall.edu/etd/descript.asp?ref=370.

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Thesis (M.S.)--Marshall University, 2003.
Title from document title page. Document formatted into pages; contains xv, 148 p. including illustrations. Includes bibliographical references (p. 131-148).

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15

Ali, S. M. "The role of phosphoproteins during development and memory formation in the chick". Thesis, Open University, 1988. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.383569.

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16

Laverdure, Guy R. J. "Growth factor regulation of a 69kDa phosphoprotein secreted by NRK- -49F cells". Thesis, McGill University, 1989. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=59535.

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Our study shows that the secretion of a major glycosylated, phosphoprotein with a molecular weight of 69kDa (pp69) is a specific marker for non-transformed NRK-49F cells. Antibody raised against pp69 recognizes, in addition to pp69, another major phosphoprotein with a molecular weight of 62kDa (pp62) secreted by RR1022 and spontaneously transformed NRK-49F cells (spt-NRK-49F). Immunoprecipitation of total cell lysates from both NRK-49F and RR1022 cells with anti-pp69 antibody detected only pp69. Treatments with: epidermal growth factor (EGF), transforming growth factor-$ beta$ (TGF-$ beta$) retinoic acid (RA), and TPA modulate the levels of pp69 present in the conditioned media. Furthermore, TPA and EGF induce the synthesis of 3 internal peptides with molecular weights of 58, 54, and 44 kDa which appear to be pre-processed forms of pp69.
Treatment of NRK-49F cells with insulin, EGF, TGF-$ beta$, PPA, levamisole and spermine clearly demonstrate alterations in the phosphorylation of pp69, concomitant with changes in extracellular phosphatase activity.

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17

Kalita, Ann Marie. "Comparison of the activities of two allelic variants of the human wildtype p53 protein". Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1997. http://www.collectionscanada.ca/obj/s4/f2/dsk2/ftp04/mq29729.pdf.

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18

Nemir, Mohamed. "Inhibition of osteopontin expression in mammary epithelial cells alters mammary gland morphogenesis". Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1998. http://www.collectionscanada.ca/obj/s4/f2/dsk1/tape10/PQDD_0020/NQ44529.pdf.

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19

Shanmugam, Vijayalakshmi. "Characterization of osteopontin in RSV transformed rat-1 cells and its role in cell transformation". Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1997. http://www.collectionscanada.ca/obj/s4/f2/dsk2/tape16/PQDD_0005/NQ37025.pdf.

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20

Naor, Naftaly. "The immune response against p53 protein in cancer patients /". Thesis, McGill University, 1993. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=69652.

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Mutations in the p53 gene are known to be associated with a wide range of human tumors. In some primary tumors and established cell lines, stable mutant p53 protein is expressed at high levels, whereas, in normal cells unstable wild-type p53 protein is expressed at very low levels. Sera from some patients with breast and colon tumors contain anti-p53 antibodies. It is unclear whether changes in p53 structure, or its increased level in tumors, causes p53 to become antigenic. In our study we tested sera from patients with various types of cancer for anti-p53 antibodies. Examination of the sera was made by Western blot, and the results were confirmed by rescreening sera with immunoprecipitation. Both techniques revealed the presence of anti-p53 antibodies in some sera from lung and ovary cancer patients, as well as in the sera from patients with breast or colon cancers. Clearly, patients with various cancer tumors are able to produce anti-p53 antibodies. It was unclear whether this humoral immune response is against mutant or wild type p53. To provide a better definition of this immune response, we have examined the anti-p53 response from cancer patients against mutant and wild type p53 in the native and denaturated state. Western blot and Immunoprecipitation analysis showed that the anti-p53 sera recognise both wild type and mutant p53 conformational and denaturation resistant epitopes. Taken together, these data demonstrate that the mutant p53 is not more antigenic than the wild type p53. This provides strong evidence that the antibody response is not directed solely against the altered conformation in mutant p53.

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21

Lee, Wai-him, i 李偉謙. "Proteomic analysis of protein phosphorylation in PC12 cells induced bypituitary adenylate cyclase activating polypeptide 38". Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2002. http://hub.hku.hk/bib/B30149885.

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22

Walker, Valerie Glynis. "Pl3-kinase mediates cSrc activation and podosome formation through the adaptor protein, AFAP-110, in response to PKC[alpha] activation". Morgantown, W. Va. : [West Virginia University Libraries], 2007. https://eidr.wvu.edu/etd/documentdata.eTD?documentid=5191.

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Thesis (Ph. D.)--West Virginia University, 2007.
Title from document title page. Document formatted into pages; contains viii, 306 p. : ill. (some col.). Vita. Includes abstract. Includes bibliographical references.

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23

Wenzel, Jens [Verfasser], i Thomas [Gutachter] Rudel. "Regulation of TLR-induced macrophage responses by cytoskeleton-associated phosphoproteins / Jens Wenzel. Gutachter: Thomas Rudel". Würzburg : Universität Würzburg, 2014. http://d-nb.info/1108780806/34.

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24

Broncel, Malgorzata [Verfasser]. "Synthetic phosphopeptides and phosphoproteins as tools for studying peptide and protein self-assembly / Malgorzata Broncel". Berlin : Freie Universität Berlin, 2011. http://d-nb.info/1025552814/34.

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25

Al-Mokhtar, Ruby. "Identification of the FERM domain containing proteins moesin, radixin and ezrin as insulin-regulated phosphoproteins". Thesis, University of Bristol, 2010. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.528102.

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26

Vogel, Elizabeth Maura. "Caged phosphopeptides and phosphoproteins : probes to dissect the role of phosphorylation in complex signaling pathways". Thesis, Massachusetts Institute of Technology, 2007. http://hdl.handle.net/1721.1/38621.

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Thesis (Ph. D.)--Massachusetts Institute of Technology, Dept. of Chemistry, 2007.
Vita.
Includes bibliographical references.
Protein phosphorylation is a central regulatory mechanism in signal transduction pathways and cellular migration. Current genetic strategies for the study of phosphorylation, including gene knockout and point mutation, are limited in providing temporal information. As a complement to these techniques, the synthesis and semisynthesis of probes that enable researchers to observe the downstream effects of kinase-mediated phosphorylation in "real time" are presented in this thesis. The release of a physiologically-relevant concentration of a phosphopeptide with temporal and spatial control is accomplished by the photolysis of a photolabile precursor, a caged phosphopeptide. The synthesis and application of NI-Fmoc-protected 1-(2-nitrophenyl) ethyl (NPE) caged phosphothreonine, serine, and tyrosine building blocks facilitate the straightforward assembly of any caged phosphopeptide through Fmoc-based solid phase peptide synthesis. Removal of the NPE caging group by irradiation with long-wavelength UV light generates a concentration burst of the corresponding phosphopeptide. In addition, the installation of a caged phosphoamino acid into a full-length, multi-domain protein, the cellular migration protein paxillin, is described. A strategy, which is applicable to any expressible protein target, is detailed for the semisynthesis of a paxillin variant with a caged phosphorylated tyrosine at residue 31 of the 557-residue protein using native chemical ligation.
(cont.) Paxillin is a 61-kDa protein known to orchestrate the interaction of signaling proteins involved in cell migration by acting as a molecular adaptor, with the creation of specific binding sites dependent on paxillin phosphorylation. Therefore, the semisynthetic probe comprises the entire paxillin macromolecule, including all other binding and localization domains, which are essential for creating a native-like system to probe the effect of phosphorylation. The comprehensive biochemical characterization of the paxillin probe and quantification of uncaging following irradiation with long-wavelength UV light are also described. Additionally, the strategy developed for the paxillin semisynthesis was applied to incorporate a different unnatural amino acid, the fluorescent chemosensing residue Sox, into a protein-domain sensor for ERK2 kinase activity. The protein domain sensor demonstrated significantly improved sensitivity for ERK2 phosphorylation over the corresponding peptide probe.
by Elizabeth Maura Vogel.
Ph.D.

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27

Brännström, Kristoffer. "Molecular dissection of established and proposed members of the Op18/Stathmin family of tubulin binding proteins /". Umeå : Univ, 2009. http://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-1949.

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28

Barker, Sharon. "The intracellular localization of mammalian DNA ligase I". Thesis, McGill University, 1996. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=23986.

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DNA replication is cruciaI for the transmission of genetic information. Understanding the enzymology involved in this complex process will allow further insight into its mechanism. Experimental evidence indicates a role for DNA ligase I in DNA replication. Techniques of molecular and cellular biology and immunology were utilized in this study to further investigate DNA ligase I and clarify its involvement and interaction with other proteins in DNA replication. Immunofluorescence studies were performed to examine the intracellular distribution of DNA ligase I. Confocal analysis of indirect immunofluorescence detection of DNA ligase I using affinity purified anti-human DNA ligase I antibodies showed nuclear localization of DNA ligase I in distinct foci resembling those structures seen in detection of centres of DNA replication and other DNA replication proteins. Immunoprecipitation experiments were performed on extracts of MDBK cells to examine possible interactions of DNA ligase I with the DNA replication cofactor, PCNA; and no interactions were detected.

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29

Phippen, Taryn Marie. "Mechanisms of Hairy-mediated transcriptional repression during Drosophila development /". Thesis, Connect to this title online; UW restricted, 2001. http://hdl.handle.net/1773/5059.

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30

Sneesby, Kyra, i n/a. "Gene Expression in Embryonic Chick Heart Development". Griffith University. School of Biomolecular and Biomedical Science, 2003. http://www4.gu.edu.au:8080/adt-root/public/adt-QGU20030924.153514.

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Establishment of the biochemical and molecular nature of cardiac development is essential for us to understand the relationship between genetic and morphological aspects of heart formation. The molecular mechanisms that underly heart development are still not clearly defined. To address this issue we have used two approaches to identify genes involved in early chick cardiac development. Differential display previously conducted in our laboratory led to the identification of two gene fragments differentially expressed in the heart that are further described in this thesis. The full-length cDNA sequence of both eukaryotic translation initiation factor-2b (eIF-2b) and NADH cytochrome b5 reductase (b5R) were isolated using library screening. The upreglation of these genes during heart development is expected given the heart is the first functional organ to form in vertebrates and protein synthesis and cell metabolism at this stage of development is maximal. Limitations in the differential display approach led to the development and optimisation of a subtractive hybridisation approach for use with small amounts of cells or tissue. To focus on cardiac gene expression during the initial phases of heart development, subtractive hybridization was performed between the cardiogenic lateral plate mesoderm of Hamburger and Hamilton stage 4 embryos and the heart primordia of stage 9 embryos. Of the 87 independent clones identified by this procedure, 59 matched known sequences with high homology, 25 matched unknown expressed sequence tag (EST) sequences with high homology, and 3 did not match any known sequence on the database. Known genes isolated included those involved in transcription, translation, cell signalling, RNA processing, and energy production. Two of these genes, high mobility group phosphoprotein A2 (HMGA2) and C1-20C, an unknown gene, were chosen for further characterisation. The role of each gene in early chick heart development and indeed development in general, was addressed using techniques such as in situ hybridisation, transfection analysis, in ovo electroporation and RNAi. HMGA2 is a nuclear phosphoprotein commonly referred to as an architectural transcription factor due to its ability to modulate DNA conformation. In keeping with this function, HMGA2/GFP fusion protein was shown to localise to the nucleus and in particular, the nucleolus. In situ hybridisation analysis suggested a role for HMGA2 in heart and somite development. HMGA2 expression was first detected at HH stage 5 in the lateral plate mesoderm, a region synonymous with cells specified to the cardiac fate. HMGA2 was also strongly expressed in the presomitic segmental plate mesoderm and as somites developed from the segmental plate mesoderm, the expression of HMGA2 showed an increasingly more restricted domain corresponding to the level of maturation of the somite. Restriction of HMGA2 expression was first detected in the dorsal region of the epithelial somite, then the dorsomedial lip of the dermomyotome, and finally the migrating epaxial myotome cells. The novel intronless gene, C1-20C, predicts a protein of 148 amino acids containing a putative zinc finger binding domain and prenyl binding motif. Zinc binding assays showed that the zinc finger domain of C1-20C/MBP fusion protein bound over six times the quantity of zinc compared to MBP alone, although not in a 1:1 stoichiometric molar ratio. C1-20C/GFP fusion protein was shown to localise to as yet unidentified intracellular cytoplasmic vesicular compartments. These compartments did not colocalise with the endosome/lysosome pathway, aparently ruling out a role for C1-20C in protein trafficking, recycling or degradation. Expression of C1-20C in the chick embryo suggests a possible role in heart and notochord development and preliminary results using siRNA suggest that C1-20C is involved in normal heart looping.

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31

Sneesby, Kyra. "Gene Expression in Embryonic Chick Heart Development". Thesis, Griffith University, 2003. http://hdl.handle.net/10072/367647.

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Establishment of the biochemical and molecular nature of cardiac development is essential for us to understand the relationship between genetic and morphological aspects of heart formation. The molecular mechanisms that underly heart development are still not clearly defined. To address this issue we have used two approaches to identify genes involved in early chick cardiac development. Differential display previously conducted in our laboratory led to the identification of two gene fragments differentially expressed in the heart that are further described in this thesis. The full-length cDNA sequence of both eukaryotic translation initiation factor-2b (eIF-2b) and NADH cytochrome b5 reductase (b5R) were isolated using library screening. The upreglation of these genes during heart development is expected given the heart is the first functional organ to form in vertebrates and protein synthesis and cell metabolism at this stage of development is maximal. Limitations in the differential display approach led to the development and optimisation of a subtractive hybridisation approach for use with small amounts of cells or tissue. To focus on cardiac gene expression during the initial phases of heart development, subtractive hybridization was performed between the cardiogenic lateral plate mesoderm of Hamburger and Hamilton stage 4 embryos and the heart primordia of stage 9 embryos. Of the 87 independent clones identified by this procedure, 59 matched known sequences with high homology, 25 matched unknown expressed sequence tag (EST) sequences with high homology, and 3 did not match any known sequence on the database. Known genes isolated included those involved in transcription, translation, cell signalling, RNA processing, and energy production. Two of these genes, high mobility group phosphoprotein A2 (HMGA2) and C1-20C, an unknown gene, were chosen for further characterisation. The role of each gene in early chick heart development and indeed development in general, was addressed using techniques such as in situ hybridisation, transfection analysis, in ovo electroporation and RNAi. HMGA2 is a nuclear phosphoprotein commonly referred to as an architectural transcription factor due to its ability to modulate DNA conformation. In keeping with this function, HMGA2/GFP fusion protein was shown to localise to the nucleus and in particular, the nucleolus. In situ hybridisation analysis suggested a role for HMGA2 in heart and somite development. HMGA2 expression was first detected at HH stage 5 in the lateral plate mesoderm, a region synonymous with cells specified to the cardiac fate. HMGA2 was also strongly expressed in the presomitic segmental plate mesoderm and as somites developed from the segmental plate mesoderm, the expression of HMGA2 showed an increasingly more restricted domain corresponding to the level of maturation of the somite. Restriction of HMGA2 expression was first detected in the dorsal region of the epithelial somite, then the dorsomedial lip of the dermomyotome, and finally the migrating epaxial myotome cells. The novel intronless gene, C1-20C, predicts a protein of 148 amino acids containing a putative zinc finger binding domain and prenyl binding motif. Zinc binding assays showed that the zinc finger domain of C1-20C/MBP fusion protein bound over six times the quantity of zinc compared to MBP alone, although not in a 1:1 stoichiometric molar ratio. C1-20C/GFP fusion protein was shown to localise to as yet unidentified intracellular cytoplasmic vesicular compartments. These compartments did not colocalise with the endosome/lysosome pathway, aparently ruling out a role for C1-20C in protein trafficking, recycling or degradation. Expression of C1-20C in the chick embryo suggests a possible role in heart and notochord development and preliminary results using siRNA suggest that C1-20C is involved in normal heart looping.
Thesis (PhD Doctorate)
Doctor of Philosophy (PhD)
School of Biomolecular and Biomedical Sciences
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32

Dädelow, Judith. "Elektronenmikroskopische Studien zur Immunlokalisation des listeriellen Oberflächenproteins ActA sowie seines zellulären Bindungspartners, des Vasodilator-stimulated Phosphoproteins (VASP) /". [S.l. : s.n.], 1999. http://www.gbv.de/dms/bs/toc/302594450.pdf.

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33

Frost, Laura Stephanie. "Characterisation of the Mitotic Golgi Phosphoproteins, TATA Modulatory Factor(TMF) and Zinc Finger Protein-Like 1 (ZFPL1)". Thesis, University of Manchester, 2009. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.503762.

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34

Labbé, Etienne. "Temperature-modulation of protein phosphorylation in cell-free extracts of alfalfa". Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1996. http://www.collectionscanada.ca/obj/s4/f2/dsk3/ftp04/MQ44093.pdf.

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35

Karlsson, Margareta. "Caveolae in insulin signalling in human and rat adipocytes /". Linköping : Univ, 2003. http://www.bibl.liu.se/liupubl/disp/disp2003/med782s.pdf.

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36

Elzi, David John. "Transcriptional properties of the Kaiso class of transcription factors /". Thesis, Connect to this title online; UW restricted, 2007. http://hdl.handle.net/1773/5027.

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37

Renström, Frida. "Fat cell insulin resistance : an experimental study focusing on molecular mechanisms in type 2 diabetes /". Umeå : Department of Public Health and Clinical Medicine, Umeå University, 2007. http://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-1078.

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38

Johnstone, Mary O. "Characterization of soluble matrix from molluscan shell with an emphasis on two major phosphoproteins from the Eastern oyster, Crassostrea virginica". Connect to this title online, 2008. http://etd.lib.clemson.edu/documents/1211398553/.

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Garaguso, Ignazio [Verfasser]. "Development of MALDI-TOF mass spectrometry based methods for the identification and molecular characterization of proteins, phosphoproteins and DNA adducts / Ignazio Garaguso". Hannover : Technische Informationsbibliothek und Universitätsbibliothek Hannover (TIB), 2011. http://d-nb.info/1019723939/34.

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Kawczyński, Wojciech. "Effects of low temperature on nuclear proteins of alfalfa". Thesis, McGill University, 1995. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=23278.

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During the present studies we attempted to answer the following questions: (i) Does low temperature alter the phosphorylation level of proteins in isolated nuclei? (ii) Does the nuclear phosphoprotein population change during a prolonged exposure of seedlings to cold? (iii) Do heat-stable proteins accumulate in the nucleus during a prolonged exposure of seedlings to cold? (iv) Are the answers to the above three questions related to freezing tolerance? A possible relationship between the observed cold-induced changes in phosphoproteins and the level of freezing tolerance was explored by comparing the results of experiments conducted on two cultivars (Apica and Trek) of alfalfa (Medicago sativa L.) which markedly differ in their capacity for cold acclimation.
We show that the phosphorylation level of several nuclear proteins is subject to rapid and reversible enhancement by low temperature. Several phosphoproteins were found to be constitutively present in the nucleus of both cultivars. The cold-induced stimulation of the phosphorylation of many of these proteins was much greater in the relatively freezing tolerant cultivar Apica than in the relatively freezing sensitive cultivar Trek. Population of nuclear phosphoproteins was found to be considerably more complex in Apica than in Trek. During a prolonged exposure of the seedlings to 4$ sp circ$C, additional phosphoproteins were imported into the nucleus of Apica seedlings but not those Trek.
Some heat-stable proteins were constitutively present in the nucleus of both cultivars. However during the 4-day cold treatment, a large accumulation of several additional heat-stable proteins was observed in the tolerant, but not the sensitive, cultivar. (Abstract shortened by UMI.)

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41

Chitta, Karnakar Reddy. "Selenium mediated arsenic toxicity modifies cytotoxicity, reactive oxygenspecies and phosphorylated proteins". University of Cincinnati / OhioLINK, 2013. http://rave.ohiolink.edu/etdc/view?acc_num=ucin1367942581.

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Russell, Tanya D. "PAT protein regulation of cytoplasmic lipid droplet formation and secretion : role of adipophilin in mammary epithelial cells /". Connect to full text via ProQuest. Limited to UCD Anschutz Medical Campus, 2008.

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Thesis (Ph.D. in Molecular Biology) -- University of Colorado Denver, 2008.
Typescript. Includes bibliographical references (leaves 134-149). Free to UCD affiliates. Online version available via ProQuest Digital Dissertations;

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43

Ghafouri, Bijar. "Proteomics of the upper airways : studies on a new lipopolysaccharide-binding protein, PLUNC /". Linköping : Dept. of Molecular and Clinical Medicine, Linköping University, 2005. http://www.bibl.liu.se/liupubl/disp/disp2005/med927s.pdf.

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Jenkins, Mark 1979. "A role for the Drosophila eIF4E binding protein during stress response /". Thesis, McGill University, 2004. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=82256.

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The Drosophila melanogaster eIF4E binding protein (d4E-BP) inhibits translation initiation and is implicated in cell growth as a downstream effector of the Drosophila insulin signaling pathway. Since d4E-BP null flies show similar growth and development to control flies, the possibility of a conditional phenotype was explored through stress treatments. Adult d4E-BP null flies show sensitivity to oxidative stress, and d4E-BP null larvae die faster than controls under starvation and protein starvation. Expressing a mutant d4E-BP that doesn't bind to eIF4E in the d4E-BP null background does not rescue this stress sensitivity, which suggests that wild-type stress resistance requires binding of d4E-BP to eIF4E.
The Drosophila forkhead transcription factor dFOXO is a transcriptional activator of d4E-BP. There is a strong reduction of d4E-BP peptide in a dFOXO null background. dFOXO null flies are also sensitive to oxidative stress, and rescue of this sensitivity through ectopic expression of UAS-d4E-BP(wt) in a dFOXO null background suggests d4E-BP is a downstream mediator of dFOXO oxidative stress resistance.

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Stenkula, Karin. "A molecular approach to insulin signalling and caveolae in primary adipocytes /". Linköping : Univ, 2006. http://www.bibl.liu.se/liupubl/disp/disp2007/med977s.pdf.

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Sparks, Cynthia A. "Cloning and Cell Cycle Analysis of NuMA, a Phosphoprotein That Oscillates Between the Nucleus and the Mitotic Spindle". eScholarship@UMMS, 1995. https://escholarship.umassmed.edu/gsbs_diss/35.

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The overall objective of this study was to identify novel proteins of the nuclear matrix in order to contribute to a better understanding of nuclear structure and organization. To accomplish this, a monoclonal antibody specific for the nuclear matrix was used to screen a human λgt11 expression library. Several cDNAs were isolated, cloned, sequenced, and shown to represent NuMA, the nuclear mitotic spindle apparatus protein. Further characterization of the gene and RNA was undertaken in an effort to obtain information about NuMA. The NuMA gene was present at a single site on human chromosome 11q13. Northern and PCR analysis of NuMA mRNA showed a major 7.2 kb transcript and minor forms of 8.0 and 3.0 kb. The minor forms were shown to be alternatively spliced although their functional significance is not yet understood. Immunofluorescence microscopy demonstrated that NuMA oscillates between the nucleus and the microtubule spindle apparatus during the mitotic cell cycle. NuMA appeared as a 200-275 kDa protein detectable in all mammalian cells except human neutrophils. To determine whether NuMA's changes in intracellular distribution correlated with post-translational modifications, the protein's phosphorylation state was examined through the cell cycle using highly synchronized cells. NuMA was a phosphoprotein in interphase and underwent additional phosphorylation events in mitosis. The mitotic phosphorylation events occurred with similar timing to lamin B (G2/M transition) and were concomitant with NuMA's release from the nucleus and its association with the mitotic spindle. However, the mitotic phosphorylation occurred in the absence of spindle formation. Dephosphorylation of NuMA did not correlate with reassociation with the nuclear matrix but occurred in two distinct steps after nuclear reformation. Based on the timing of these events, phosphorylation may playa role in nuclear processes. In conclusion, the work in this dissertation identified NuMA, a nuclear matrix protein and showed that it is phosphorylated during the cell cycle and may be important for nuclear events such as nuclear organization, transcription, or initiation of DNA replication at G1/S.

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Thurfjell, Niklas. "p63 - from expression to function : studies of normal oral mucosa and squamous cell carcinoma of the head and neck /". Doctoral thesis, Umeå : Univ, 2007. http://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-998.

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Семенина, Іванна Петрівна. "Отримання кальцієвмісних природних харчових функціональних інгредієнтів". Thesis, Тернопільський національний технічний університет імені Івана Пулюя, 2017. http://elartu.tntu.edu.ua/handle/123456789/19306.

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В дипломній роботі для виділення фосфопептидів з продуктів протеолізу загального казеїну запропоновано використовувати селективне осадження їх іонами Са2+ і спиртами. За результатами гель-фільтрації встановлено, що природні кальцієвмісні фосфопептиди, які можуть бути використані як функціональні харчові інгерієнти, утворюються при використанні етанолу.
In thisgraduation degree for extracting phosphopeptidesfrom the products of proteolysisof total casein,their selective precipitation with Са2+ ions and alcohols is suggested. By the result of gel filtration it was established thatnatural calcium-containingphosphopeptides that may be used as functional food ingredients are formed with using ethanol.

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Bennett, Clare Suzanne. "Characterisation of secreted phosphoprotein 24". Thesis, University of Leicester, 2001. http://hdl.handle.net/2381/30334.

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Secreted phosphoprotein 24 (spp24) is a novel 24-kDa non-collagenous protein that was originally isolated from the acid demineralised extract of bovine cortical bone (Hu et al. 1995). The presence of spp24 in bone immediately suggested a potential role for the protein in the processes that occurred there. The N-terminal segment of the protein is related in sequence to the cystatin family of thiol protease inhibitors. It was therefore suggested that spp24 might inhibit thiol protease activity during bone turnover (Hu et al. 1995). Three million people in the UK suffer from osteoporosis (National Osteoporosis Society estimated figure) and their care and treatment costs the NHS and the taxpayer Â£942 million every year (Dolan and Torgerson 1998). Therefore, it is essential that we begin to understand the genetic basis and the factors that can predispose people, to osteoporosis and many other bone diseases. If spp24 has a functional role in the process of bone remodelling it is likely that it may influence the development or severity of osteoporosis. This study determines the human SPP2 gene, encoding the spp24 protein, to comprise 8 exons with apparently TATA-less promoter. The gene is shown to have multiple transcription initiation sites, which demonstrate some tissue specificity. An extensive expression study was carried out on the human and mouse gene encoding spp24, indicating that the gene has an expression pattern of a tissue-specific nature, being expressed predominantly in liver. Theoretical studies and computational methods were used to analyse spp24 from several species and proteins showing homology to spp24. These studies gave a good indication of the areas of the protein and specific residues that are likely to be critical to the function of spp24. The results supported the speculation that spp24 does not act as a typical cystatin, but instead is likely to have a fetuin-like function or an antimicrobial function.

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Sweet, Steve M. M. "Phosphoprotein analysis by mass spectrometry". Thesis, University of Manchester, 2005. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.538385.

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