Artykuły w czasopismach: „Pa4409”

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Vercammen, Ken, Qing Wei, Daniel Charlier, Andreas Dötsch, Susanne Haüssler, Sebastian Schulz, Francesca Salvi i in. "Pseudomonas aeruginosa LysR PA4203 Regulator NmoR Acts as a Repressor of the PA4202nmoAGene, Encoding a Nitronate Monooxygenase". Journal of Bacteriology 197, nr 6 (10.11.2014): 1026–39. http://dx.doi.org/10.1128/jb.01991-14.

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The PA4203 gene encodes a LysR regulator and lies between theppgLgene (PA4204), which encodes a periplasmic gluconolactonase, and, in the opposite orientation, the PA4202 (nmoA) gene, coding for a nitronate monooxygenase, andddlA(PA4201), encoding ad-alanine alanine ligase. The intergenic regions between PA4203 andppgLand between PA4203 andnmoAare very short (79 and 107 nucleotides, respectively). Here we show that PA4203 (nmoR) represses its own transcription and the expression ofnmoA. A chromatin immunoprecipitation analysis showed the presence of a single NmoR binding site betweennmoAandnmoR, which was confirmed by electrophoretic mobility shift assays (EMSAs) with the purified NmoR protein. Despite this observation, a transcriptome analysis revealed more genes to be affected in annmoRmutant, including genes known to be part of the MexT LysR activator regulon. The PA1225 gene, encoding a quinone oxidoreductase, was the most highly upregulated gene in thenmoRdeletion mutant, independently of MexT. Finally, deletion of thenmoAgene resulted in an increased sensitivity of the cells to 3-nitropropionic acid (3-NPA), confirming the role of the nitronate monooxygenase protein in the detoxification of nitronate.

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Shen, Lixin, Lang Gao, Mengjiao Yang, Jian Zhang, Yulu Wang, Yuqi Feng, Liping Wang i Shiwei Wang. "Deletion of the PA4427-PA4431 Operon of Pseudomonas aeruginosa PAO1 Increased Antibiotics Resistance and Reduced Virulence and Pathogenicity by Affecting Quorum Sensing and Iron Uptake". Microorganisms 9, nr 5 (14.05.2021): 1065. http://dx.doi.org/10.3390/microorganisms9051065.

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The respiratory chain is very important for bacterial survival and pathogenicity, yet the roles of the respiratory chain in P. aeruginosa remain to be fully elucidated. Here, we not only proved experimentally that the operon PA4427-PA4431 of Pseudomonas aeruginosa PAO1 encodes respiratory chain complex III (cytobc1), but also found that it played important roles in virulence and pathogenicity. PA4429–31 deletion reduced the production of the virulence factors, including pyocyanin, rhamnolipids, elastase, and extracellular polysaccharides, and it resulted in a remarkable decrease in pathogenicity, as demonstrated in the cabbage and Drosophila melanogaster infection models. Furthermore, RNA-seq analysis showed that PA4429–31 deletion affected the expression levels of the genes related to quorum-sensing systems and the transport of iron ions, and the iron content was also reduced in the mutant strain. Taken together, we comprehensively illustrated the function of the operon PA4427–31 and its application potential as a treatment target in P. aeruginosa infection.

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Wiegand, Irith, Alexandra K. Marr, Elena B. M. Breidenstein, Kristen N. Schurek, Patrick Taylor i Robert E. W. Hancock. "Mutator Genes Giving Rise to Decreased Antibiotic Susceptibility in Pseudomonas aeruginosa". Antimicrobial Agents and Chemotherapy 52, nr 10 (28.07.2008): 3810–13. http://dx.doi.org/10.1128/aac.00233-08.

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ABSTRACT Screening of the PA14 genomic transposon mutant library for resistance to ceftazidime, tobramycin, and ciprofloxacin led to the discovery of several mutants that appeared in more than one screen. Testing of the frequency of mutation to ciprofloxacin resistance revealed previously known mutator genes, including mutS and mutL, as well as mutators that have not yet been described for P. aeruginosa, including PA3958 and RadA (PA4609).

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Otaegi, Itziar, Nora Aramburu, Alejandro Müller i Gonzalo Guerrica-Echevarría. "Novel Biobased Polyamide 410/Polyamide 6/CNT Nanocomposites". Polymers 10, nr 9 (4.09.2018): 986. http://dx.doi.org/10.3390/polym10090986.

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Biobased polyamide 410 (PA410)/multiwall carbon nanotube (CNT) nanocomposites (NCs) were obtained by melt-mixing in a twin screw extruder a Polyamide 6 (PA6)-based masterbatch (with 15 wt % CNT content) with neat PA410. Directly mixed PA410/CNT NCs were also obtained for comparison purposes. Transmision Electronic Microscopy (TEM) observation and conductivity measurements demonstrated that a good dispersion of CNTs was obtained, which was probably induced by the full miscibility between PA410 and PA6 (in the concentration range employed here), as ascertained by Differential Scanning Calorimetry (DSC) tests. As a result, the PA410/PA6/CNT NCs showed superior mechanical behaviour (≈10% Young’s modulus increase with a 4 wt % CNT content) than the binary PA410/CNT NCs (≈5% Young’s modulus increase with a 6 wt % CNT content), as well as superior electrical behaviour, with maximum conductivity values of approximately three orders of magnitude higher than in the binary PA410/CNT system, and lower percolation threshold values (0.65 wt % CNT content vs. 3.98 wt % CNT). The good dispersion and enhanced mechanical and electrical properties of these novel biobased nanocomposites, broadens their potential applications, such as electrical and electronics (E&E) or automotive industries.

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Southey-Pillig, Christopher J., David G. Davies i Karin Sauer. "Characterization of Temporal Protein Production in Pseudomonas aeruginosa Biofilms". Journal of Bacteriology 187, nr 23 (1.12.2005): 8114–26. http://dx.doi.org/10.1128/jb.187.23.8114-8126.2005.

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ABSTRACT Phenotypic and genetic evidence supporting the notion of biofilm formation as a developmental process is growing. In the present work, we provide additional support for this hypothesis by identifying the onset of accumulation of biofilm-stage specific proteins during Pseudomonas aeruginosa biofilm maturation and by tracking the abundance of these proteins in planktonic and three biofilm developmental stages. The onset of protein production was found to correlate with the progression of biofilms in developmental stages. Protein identification revealed that proteins with similar function grouped within similar protein abundance patterns. Metabolic and housekeeping proteins were found to group within a pattern separate from virulence, antibiotic resistance, and quorum-sensing-related proteins. The latter were produced in a progressive manner, indicating that attendant features that are characteristic of biofilms such as antibiotic resistance and virulence may be part of the biofilm developmental process. Mutations in genes for selected proteins from several protein production patterns were made, and the impact of these mutations on biofilm development was evaluated. The proteins cytochrome c oxidase, a probable chemotaxis transducer, a two-component response regulator, and MexH were produced only in mature and late-stage biofilms. Mutations in the genes encoding these proteins did not confer defects in growth, initial attachment, early biofilm formation, or twitching motility but were observed to arrest biofilm development at the stage of cell cluster formation we call the maturation-1 stage. The results indicated that expression of theses genes was required for the progression of biofilms into three-dimensional structures on abiotic surfaces and the completion of the biofilm developmental cycle. Reverse transcription-PCR analysis confirmed the detectable change in expression of the respective genes ccoO, PA4101, and PA4208. We propose a possible mechanism for the role of these biofilm-specific proteins in biofilm formation.

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Otaegi, Itziar, Nora Aranburu i Gonzalo Guerrica-Echevarría. "Attaining Toughness and Reduced Electrical Percolation Thresholds in Bio-Based PA410 by Combined Addition of Bio-Based Thermoplastic Elastomers and CNTs". Polymers 13, nr 19 (5.10.2021): 3420. http://dx.doi.org/10.3390/polym13193420.

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Multi-walled carbon nanotubes (CNTs) were added to provide electrical conductivity to bio-based polymer blends with improved toughness (based on commercially available Pebax thermoplastic elastomers and bio-based polyamide 4,10). A preliminary study including three different Pebax grades was carried out to select the grade and the composition that would best improve the impact properties of PA410. Thus, tough multiphasic PA/Pebax/CNT nanocomposites (NCs) with enhanced electrical conductivity were obtained. The CNTs were added either: (1) in the form of pristine nanotubes or (2) in the form of a PA6-based masterbatch. Hence, PA410/Pebax/CNT ternary NCs and PA410/PA6/Pebax/CNT quaternary NCs were obtained, respectively, up to a CNT content of 1 wt%. The ternary and quaternary NCs both showed similar mechanical and electrical properties. The electrical percolation threshold decreased with respect to previously studied corresponding NCs without Pebax, i.e., PA410/CNT and PA410/PA6/CNT, due to the partial volume exclusion effect of Pebax over the CNTs that were dispersed mainly in the PA matrix; materials with percolation concentrations as low as 0.38 wt% were obtained. With respect to mechanical properties, contrary to the NCs without Pebax, all the PA/Pebax/CNT NCs showed a ductile behavior and impact strength values that were from three to five-fold higher than that of the pure PA410.

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Weaver, Valerie B., i Roberto Kolter. "Burkholderia spp. Alter Pseudomonas aeruginosa Physiology through Iron Sequestration". Journal of Bacteriology 186, nr 8 (15.04.2004): 2376–84. http://dx.doi.org/10.1128/jb.186.8.2376-2384.2004.

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ABSTRACT Pseudomonas aeruginosa and members of the Burkholderia cepacia complex often coexist in both the soil and the lungs of cystic fibrosis patients. To gain an understanding of how these different species affect each other's physiology when coexisting, we performed a screen to identify P. aeruginosa genes that are induced in the presence of Burkholderia. A random gene fusion library was constructed in P. aeruginosa PA14 by using a transposon containing a promoterless lacZ gene. Fusion strains were screened for their ability to be induced in the presence of Burkholderia strains in a cross-streak assay. Three fusion strains were induced specifically by Burkholderia species; all three had transposon insertions in genes known to be iron regulated. One of these fusion strains, containing a transposon insertion in gene PA4467, was used to characterize the inducing activity from Burkholderia. Biochemical and genetic evidence demonstrate that ornibactin, a siderophore produced by nearly all B. cepacia strains, can induce P. aeruginosa PA4467. Significantly, PA4467 is induced early in coculture with an ornibactin-producing but not an ornibactin-deficient B. cepacia strain, indicating that ornibactin can be produced by B. cepacia and detected by P. aeruginosa when the two species coexist.

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Chen, Lin, i Kangmin Duan. "A PhoPQ-Regulated ABC Transporter System Exports Tetracycline in Pseudomonas aeruginosa". Antimicrobial Agents and Chemotherapy 60, nr 5 (7.03.2016): 3016–24. http://dx.doi.org/10.1128/aac.02986-15.

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ABSTRACTPseudomonas aeruginosais an important human pathogen whose infections are difficult to treat due to its high intrinsic resistance to many antibiotics. Here, we show that the disruption of PA4456, encoding the ATP binding component of a putative ATP-binding cassette (ABC) transporter, increased the bacterium's susceptible to tetracycline and other antibiotics or toxic chemicals. Fluorescence spectroscopy and antibiotic accumulation tests showed that the interruption of the ABC transporter caused increased intracellular accumulation of tetracycline, demonstrating a role of the ABC transporter in tetracycline expulsion. Site-directed mutagenesis proved that the conserved residues of E170 in the Walker B motif and H203 in the H-loop, which are important for ATP hydrolysis, were essential for the function of PA4456. Through a genome-wide search, the PhoPQ two-component system was identified as a regulator of the computationally predicted PA4456-4452 operon that encodes the ABC transporter system. A >5-fold increase of the expression of this operon was observed in thephoQmutant. The results obtained also show that the expression of thephzA1B1C1D1E1operon and the production of pyocyanin were significantly higher in the ABC transporter mutant, signifying a connection between the ABC transporter and pyocyanin production. These results indicated that the PhoPQ-regulated ABC transporter is associated with intrinsic resistance to antibiotics and other adverse compounds inP. aeruginosa, probably by extruding them out of the cell.

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Otaegi, Itziar, Nora Aranburu, Maider Iturrondobeitia, Julen Ibarretxe i Gonzalo Guerrica-Echevarría. "The Effect of the Preparation Method and the Dispersion and Aspect Ratio of CNTs on the Mechanical and Electrical Properties of Bio-Based Polyamide-4,10/CNT Nanocomposites". Polymers 11, nr 12 (11.12.2019): 2059. http://dx.doi.org/10.3390/polym11122059.

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Bio-based polymeric nanocomposites (NCs) with enhanced electrical conductivity and rigidity were obtained by adding multi-walled carbon nanotubes (CNTs) to a commercial bio-based polyamide 4,10 (PA410). Two different types of commercial CNTs (Cheap Tubes and Nanocyl NC7000TM) and two different preparation methods (using CNTs in powder form and a PA6-based masterbatch, respectively) were used to obtain melt-mixed PA410/CNT NCs. The effect of the preparation method as well as the degree of dispersion and aspect ratio of the CNTs on the electrical and mechanical properties of the processed NCs was studied. Superior electrical and mechanical behavior was observed in the Nanocyl CNTs-based NCs due to the enhanced dispersion and higher aspect ratio of the nanotubes. A much more significant reduction in aspect ratio was observed in the Cheap Tubes CNTs than in the Nanocyl CNTs. This was attributed to the fact that the shear stress applied during melt processing reduced the length of the CNTs to similar lengths in all cases, which pointed to the diameter of the CNTs as the key factor determing the properties of the NCs. The PA6 in the ternary PA410/PA6/CNT system led to improved Young’s modulus values because the reinforcing effect of CNTs was greater in PA6 than in PA410.

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HAYASHI, FUMIAKI, KAZUO NAGASHIMA, YOSHIHIRO TERUI, YOSHIMI KAWAMURA, KOICHI MATSUMOTO i HIROSHI ITAZAKI. "The structure of PA48009: The revised structure of duramycin." Journal of Antibiotics 43, nr 11 (1990): 1421–30. http://dx.doi.org/10.7164/antibiotics.43.1421.

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Kopec, Jolanta, Robert Schnell i Gunter Schneider. "Structure of PA4019, a putative aromatic acid decarboxylase fromPseudomonas aeruginosa". Acta Crystallographica Section F Structural Biology and Crystallization Communications 67, nr 10 (24.09.2011): 1184–88. http://dx.doi.org/10.1107/s174430911102923x.

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Huang, Bixing, Cynthia B. Whitchurch i John S. Mattick. "FimX, a Multidomain Protein Connecting EnvironmentalSignals to Twitching Motility in Pseudomonasaeruginosa". Journal of Bacteriology 185, nr 24 (15.12.2003): 7068–76. http://dx.doi.org/10.1128/jb.185.24.7068-7076.2003.

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ABSTRACT Twitching motility is a form of surface translocation mediated by the extension, tethering, and retraction of type IV pili. Three independent Tn5-B21 mutations of Pseudomonas aeruginosa with reduced twitching motility were identified in a new locus which encodes a predicted protein of unknown function annotated PA4959 in the P. aeruginosa genome sequence. Complementation of these mutants with the wild-type PA4959 gene, which we designated fimX, restored normal twitching motility. fimX mutants were found to express normal levels of pilin and remained sensitive to pilus-specific bacteriophages, but they exhibited very low levels of surface pili, suggesting that normal pilus function was impaired. The fimX gene product has a molecular weight of 76,000 and contains four predicted domains that are commonly found in signal transduction proteins: a putative response regulator (CheY-like) domain, a PAS-PAC domain (commonly involved in environmental sensing), and DUF1 (or GGDEF) and DUF2 (or EAL) domains, which are thought to be involved in cyclic di-GMP metabolism. Red fluorescent protein fusion experiments showed that FimX is located at one pole of the cell via sequences adjacent to its CheY-like domain. Twitching motility in fimX mutants was found to respond relatively normally to a range of environmental factors but could not be stimulated by tryptone and mucin. These data suggest that fimX is involved in the regulation of twitching motility in response to environmental cues.

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Akiyama, Tatsuya, Kerry S. Williamson, Robert Schaefer, Shawna Pratt, Connie B. Chang i Michael J. Franklin. "Resuscitation ofPseudomonas aeruginosafrom dormancy requires hibernation promoting factor (PA4463) for ribosome preservation". Proceedings of the National Academy of Sciences 114, nr 12 (7.03.2017): 3204–9. http://dx.doi.org/10.1073/pnas.1700695114.

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Gonzalez de Gortari, Mateo, Manjusri Misra, Stefano Gregori i Amar K. Mohanty. "Statistical Design of Biocarbon Reinforced Sustainable Composites from Blends of Polyphthalamide (PPA) and Polyamide 4,10 (PA410)". Molecules 26, nr 17 (4.09.2021): 5387. http://dx.doi.org/10.3390/molecules26175387.

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A full factorial design with four factors (the ratio of polyphthalamide (PPA) and polyamide 4,10 (PA410) in the polymer matrix, content percent of biocarbon (BioC), the temperature at which it was pyrolyzed and the presence of a chain extender (CE)), each factor with two levels (high and low), was carried out to optimize the mechanical properties of the resulting composites. After applying a linear model, changes in tensile strength, elongation at break and impact energy were not statistically significant within the considered material space, while the ones in the flexural modulus, the tensile modulus, density and heat deflection temperature (HDT) were. The two most influential factors were the content of BioC and its pyrolysis temperature, followed by the content of PPA. The affinity of PPA with a high-temperature biocarbon and the affinity of PA410 with a lower-temperature biocarbon, appear to explain the mechanical properties of the resulting composites. The study also revealed that the addition of CE hindered the mechanical properties. By maximizing the flexural modulus, tensile modulus and HDT, while minimizing the density, the optimal composite predicted is an 80 [PPA:PA410 (25:75)] wt% polymer composite, with 20 wt% of a BioC, pyrolyzed at a calculated 823 °C.

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Salvatore, S., A. Ripepi, P. Wagner, C. Luini, L. Morando, V. Portunato i L. Nespoli. "PA49 CAN WE PREVENT PEDIATRIC NOSOCOMIAL GASTROENTERITIS?" Digestive and Liver Disease 42 (październik 2010): S361. http://dx.doi.org/10.1016/s1590-8658(10)60643-3.

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Zhu, Yingxiao, Zenglin Yuan i Lichuan Gu. "Structural basis for the regulation of chemotaxis by MapZ in the presence of c-di-GMP". Acta Crystallographica Section D Structural Biology 73, nr 8 (28.07.2017): 683–91. http://dx.doi.org/10.1107/s2059798317009998.

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The bacterial second messenger cyclic diguanylate monophosphate (c-di-GMP) mediates multiple aspects of bacterial physiology through binding to various effectors. In some cases, these effectors are single-domain proteins which only contain a PilZ domain. It remains largely unknown how single-domain PilZ proteins function and regulate their downstream targets. Recently, a single-domain PilZ protein, MapZ (PA4608), was identified to inhibit the activity of the methyltransferase CheR1. Here, crystal structures of the C-terminal domain of CheR1 containing SAH and of CheR1 in complex with c-di-GMP-bound MapZ are reported. It was observed that the binding site of MapZ in CheR1 partially overlaps with the SAH/SAM-binding pocket. Consequently, binding of MapZ blocks SAH/SAM binding. This provides direct structural evidence on the mechanism of inhibition of CheR1 by MapZ in the presence of c-di-GMP.

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Tarighi, Saeed, Qing Wei, Miguel Cámara, Paul Williams, Matthew P. Fletcher, Tommi Kajander i Pierre Cornelis. "The PA4204 gene encodes a periplasmic gluconolactonase (PpgL) which is important for fitness of Pseudomonas aeruginosa". Microbiology 154, nr 10 (1.10.2008): 2979–90. http://dx.doi.org/10.1099/mic.0.2008/018465-0.

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Sarkisova, Svetlana A., Shalaka R. Lotlikar, Manita Guragain, Ryan Kubat, John Cloud, Michael J. Franklin i Marianna A. Patrauchan. "A Pseudomonas aeruginosa EF-Hand Protein, EfhP (PA4107), Modulates Stress Responses and Virulence at High Calcium Concentration". PLoS ONE 9, nr 6 (11.06.2014): e98985. http://dx.doi.org/10.1371/journal.pone.0098985.

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Ramani, A. N., A. M. Ariffin, Gobinath Vijian i Ahmad Basri Abd Ghani. "The Effects of Nano Fillers on Space Charge Distribution in Cross-Linked Polyethylene". International Journal of Electrical and Computer Engineering (IJECE) 7, nr 6 (1.12.2017): 3147. http://dx.doi.org/10.11591/ijece.v7i6.pp3147-3152.

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The performance of polymeric insulation will be distorted by the accumulation of space charge. This will lead to local electric field enhancement within the insulation material that can cause degradation and electrical breakdown. The introduction of nanofillers in the insulation material is expected to reduce the space charge effect. However, there is a need to analyze potential nanofillers to determine the best option. Therefore, the objective of this research work is to examine two types of nanofillers for Cross-Linked Polyethylene (XLPE); Zinc Oxide (ZnO) and Acrylic (PA40). The effects of these nanofillers were measured using the Pulsed-Electro Acoustic (PEA) method. The development of space charge is observed at three different DC voltage levels in room temperature. The results show that hetero charge distribution is dominant in pure XLPE materials. The use of both nanofiller types have significant effect in decreasing the space charge accumulation. With nanofillers, the charge profile changed to homo-charge distribution, suppressing the space charge formation. Comparison between both the nanofillers show that PA40 has better suppression performance than ZnO.

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Kim, Do Wan, Ki Seog Lee i Young Min Chi. "Crystal structure of hypothetical protein PA4202 from Pseudomonas aeruginosa PAO1 in complex with nitroethane as a nitroalkane substrate". Biochemical and Biophysical Research Communications 503, nr 1 (wrzesień 2018): 330–37. http://dx.doi.org/10.1016/j.bbrc.2018.06.024.

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Li, Xian-Zhi, Keith Poole i Hiroshi Nikaido. "Contributions of MexAB-OprM and an EmrE Homolog to Intrinsic Resistance of Pseudomonas aeruginosa to Aminoglycosides and Dyes". Antimicrobial Agents and Chemotherapy 47, nr 1 (styczeń 2003): 27–33. http://dx.doi.org/10.1128/aac.47.1.27-33.2003.

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ABSTRACT Of the six putative small multidrug resistance (SMR) family proteins of Pseudomonas aeruginosa, a protein encoded by the PA4990 gene (emrE Pae) shows the highest identity to the well-characterized EmrE efflux transporter of Escherichia coli. Reverse transcription-PCR confirmed the expression of emrE Pae in the wild-type strain of P. aeruginosa. Using isogenic emrE Pae, mexAB-oprM, and/or mexB deletion mutants, the contributions of the EmrE protein and the MexAB-OprM efflux system to drug resistance in P. aeruginosa were assessed by a drug susceptibility test carried out in a low-ionic-strength medium, Difco nutrient broth. We found that EmrEPae contributed to intrinsic resistance not only to ethidium bromide and acriflavine but also to aminoglycosides. In this low-ionic-strength medium, MexAB-OprM was also shown to contribute to aminoglycoside resistance, presumably via active efflux. Aminoglycoside resistance caused by these two pumps could not be demonstrated in high-ionic-strength media, such as Luria broth or Mueller-Hinton broth. The EmrE-dependent efflux of ethidium bromide was confirmed by a continuous fluorescence assay.

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McMullen, Michael D. "Three Genetic Loci Control Resistance to Wheat Streak Mosaic Virus in the Maize Inbred Pa405". Molecular Plant-Microbe Interactions 7, nr 6 (1994): 708. http://dx.doi.org/10.1094/mpmi-7-0708.

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Gonzalez de Gortari, Mateo, Feng Wu, Amar K. Mohanty i Manjusri Misra. "Evaluating the Performance of a Semiaromatic/Aliphatic Polyamide Blend: The Case for Polyphthalamide (PPA) and Polyamide 4,10 (PA410)". Polymers 13, nr 19 (2.10.2021): 3391. http://dx.doi.org/10.3390/polym13193391.

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This paper studies the structure–property–processing relationship of polyphthalamide (PPA) PPA/polyamide 4,10 (PA410) blends, via co-relating their thermal-mechanical properties with their morphology, crystallization, and viscoelastic properties. When compared to neat PPA, the blends show improved processability with a lower processing temperature (20 °C lower than neat PPA) along with a higher modulus/strength and heat deflection temperature (HDT). The maximum tensile modulus is that of the 25PPA/75PA410 blend, ~3 GPa, 25% higher than neat PPA (~2.4 GPa). 25PPA/75PA410 also exhibits the highest HDT (136 °C) among all the blends, being 11% more than PPA (122 °C). The increase in the thermo-mechanical properties of the blends is explained by the partial miscibility between the two polymers. The blends improve the processing performance of PPA and broaden its applicability.

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Habazettl, Judith, Martin G. Allan, Urs Jenal i Stephan Grzesiek. "Solution Structure of the PilZ Domain Protein PA4608 Complex with Cyclic di-GMP Identifies Charge Clustering as Molecular Readout". Journal of Biological Chemistry 286, nr 16 (10.02.2011): 14304–14. http://dx.doi.org/10.1074/jbc.m110.209007.

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Tronconi, G. M., B. Parma, P. Corsin, G. Nebbia, G. Weber i G. Barera. "PA40 MULTIPLE AUTOIMMUNE DISEASES IN CELIAC DISEASE: A CASE REPORT". Digestive and Liver Disease 41 (październik 2009): S237. http://dx.doi.org/10.1016/s1590-8658(09)60545-4.

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Shan, Zhiying, Haijin Xu, Xingqi Shi, Yan Yu, Hongming Yao, Xiuming Zhang, Yanling Bai, Caichang Gao, Per E. J. Saris i Mingqiang Qiao. "Identification of two new genes involved in twitching motility in Pseudomonas aeruginosa". Microbiology 150, nr 8 (1.08.2004): 2653–61. http://dx.doi.org/10.1099/mic.0.27131-0.

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Mu transposition complexes were used for transposon mutagenesis of Pseudomonas aeruginosa strain PA68. Mu DNA transposition complexes were assembled with MuA transposase and an artificial mini-Mu transposon in vitro, and introduced into Pseudomonas aeruginosa by electroporation. Eight mutants deficient in twitching motility were isolated. Southern blotting confirmed that the insertions had occurred as single events. DNA sequencing of the region flanking the insertion in the twitching-motility mutants revealed that the mini-Mu transposon had inserted into six different genes, PAO171, PA1822, PAO413, PA4959, PA4551 and PA5040. Four of these have previously been proven to be needed for twitching motility, whereas the PA1822 and PA0171 genes have not previously been shown to be required for twitching motility. The twitching-motility defect in the PA1822 mutant was partially complemented by providing the PA1822 gene in trans, and the defect in the PA0171 mutant was fully complemented when PA0171 was provided. A PA0171 mutant and a PA1822 mutant were constructed by gene replacement in the P. aeruginosa PAO1 strain. These mutants were deficient in twitching motility, showing that both the PA1822 and the PA0171 gene are involved in twitching motility.

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Di Nardo, G., F. Valitutti, F. Ferrari, S. Mallardo, P. Rossi, C. Di Camillo, I. Celletti, M. Barbato, A. Marcheggiano i S. Cucchiara. "PA44 AN UNUSUAL GUT BLEEDING IN CHILDHOOD SUCCESSFULLY TREATED WITH OCTREOTIDE". Digestive and Liver Disease 42 (październik 2010): S359. http://dx.doi.org/10.1016/s1590-8658(10)60638-x.

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Bulegeya, Victoria B., Mark W. Jones, Tryphone G. Muhamba, Biswanath Das, Peter R. Thomison, David M. Francis i Margaret G. Redinbaugh. "Selecting for Coupling-Phase Recombination Between Potyvirus Resistance and White Endosperm Colour in Maize Preferred by Farmers in Sub-Saharan Africa (SSA)". Afrika Focus 32, nr 2 (27.02.2019): 39–48. http://dx.doi.org/10.1163/2031356x-03202004.

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Maize lethal necrosis (MLN) disease caused by a combined infection of Maize chlorotic mottle virus (MCMV) and any cereal infecting potyvirus is a threat to food security in Sub-Saharan Africa (SSA). Resistance to potyvirus has been extensively studied and Mdm1 gene for potyvirus resistance on chromosome 6 of maize is linked to Y1 gene for maize endosperm colour. This study is aimed at selecting for coupling-phase recombination of potyvirus resistance and white endosperm colour. White susceptible maize lines CML333 and CML277 were crossed with a yellow resistant line, Pa405, to produce F1 and F2 progenies. Progenies were screened using molecular markers to recover 22 white endosperm recombinants. 22 selections were advanced to F3 recombinant families, and 10 were assayed for their responses to Maize dwarf mosaic virus (MDMV) and Sugarcane mosaic virus (SCMV). Four families segregated for SCMV resistance, selection of homozygous recombinants within these families will provide lines appropriate for improving lines with resistance to SCMV and MLN resistance in SSA.

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Ramelot, Theresa A., Adelinda Yee, John R. Cort, Anthony Semesi, Cheryl H. Arrowsmith i Michael A. Kennedy. "NMR structure and binding studies confirm that PA4608 from Pseudomonas aeruginosa is a PilZ domain and a c-di-GMP binding protein". Proteins: Structure, Function, and Bioinformatics 66, nr 2 (9.11.2006): 266–71. http://dx.doi.org/10.1002/prot.21199.

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Muñoz-Clares, Rosario A., Arline Fernández-Silva, Carlos Mújica-Jiménez i Sebastian Martínez-Flores. "Substrate Specificity in Promiscuous Enzymes: The Case of the Aminoaldehyde Dehydrogenases from Pseudomonas aeruginosa". Journal of the Mexican Chemical Society 67, nr 3 (1.07.2023): 240–50. http://dx.doi.org/10.29356/jmcs.v67i3.2022.

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Abstract. Substrate specificity is instrumental in enzyme catalysis and a major determinant of the enzyme’s physiological role. Nevertheless, the degree of substrate specificity may vary in an ample range; some enzymes exhibit very high specificity while others show a relaxed specificity or promiscuity. The latter is used by evolution for the emergence of new enzymes able to perform novel metabolic roles. The basis of substrate specificity is substrate recognition, which is achieved in the enzyme active site by chemical and structural mechanisms. Here, we exemplify that specificity may exist within promiscuity by comparatively analyzing kinetic and structural data of the four Pseudomonas aeruginosa aminoaldehyde dehydrogenases—PA5373, PA5312, PA4189, and PA0219. Despite their apparent substrate promiscuity, we found that these enzymes show a significant degree of substrate specificity. They have evolved to preferentially oxidize different aminoaldehydes, even though each of them can use as in vitro substrates most of the aminoaldehydes preferred by the others. We focus on the role played in these enzymes by two active-site residues—one acidic and the other aromatic, both belonging to the so-called “anchor” loop—in binding the aldehyde amino group, as well as on the importance of the anchor loop conformation in defining the size and shape of the active-site cavity. Our results support the notion that natural selection has fine-tuned the active-site structural and chemical features of the P. aeruginosa AMADH enzymes to the structural and chemical features of their physiological aminoaldehydes substrates. Resumen. La especificidad de sustrato es fundamental para la catálisis enzimática y un importante determinante del papel fisiológico de una enzima. Sin embargo, el grado de especificidad de las enzimas puede variar en un amplio intervalo; algunas enzimas exhiben una especificidad estricta por sus sustratos mientras que otras exhiben una especicificidad relajada o promiscuidad. Esto último es utilizado por la evolución para que emerjan nuevas enzimas capaces de llevar a cabo funciones metabólicas novedosas. La base de la especificidad es el reconocimiento del sustrato, lo que se logra en el sitio activo de la enzima mediante mecanismos químicos y estructurales. En este trabajo mostramos que la especificidad puede existir dentro de la promiscuidad, analizando resultados cinéticos y estructurales de las cuatro aminoaldehído deshidrogenasas de Pseudomonas aeruginosa—PA5373, PA5312, PA4189 y PA0219. A pesar de su aparente promiscuidad, encontramos que estas enzimas presentan un alto grado de especificidad. Han evolucionado para oxidar preferencialmente algunos aminoaldehídos, aunque cada una de ellas pueda in vitro usar como sustratos la mayoría de aminoaldehídos preferidos por las otras. Enfocamos nuestro análisis en el papel que desempeñan dos residuos del sitio activo—uno ácido y el otro aromático, ambos pertenecientes a la llamada asa “ancla”—en la unión de los grupos aminos de sus aldehídos sustrato, así como en la importancia de la conformación de esta asa para definir el tamaño y la forma de la cavidad del sitio activo. Nuestros resultados apoyan la idea de que la selección natural ha finamente ajustado las características químicas y estructurales del sito activo de las AMADHs a las características químicas y estructurales de sus sustratos aldehídos fisiológicos.

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McElvaney, Oliver J., Niamh O’Reilly, Michelle White, Noreen Lacey, Kerstin Pohl, Tanja Gerlza, David A. Bergin i in. "The effect of the decoy molecule PA401 on CXCL8 levels in bronchoalveolar lavage fluid of patients with cystic fibrosis". Molecular Immunology 63, nr 2 (luty 2015): 550–58. http://dx.doi.org/10.1016/j.molimm.2014.10.013.

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Seo, Jin, Anja Brencic i Andrew J. Darwin. "Analysis of Secretin-Induced Stress in Pseudomonas aeruginosa Suggests Prevention Rather than Response and Identifies a Novel Protein Involved in Secretin Function". Journal of Bacteriology 191, nr 3 (21.11.2008): 898–908. http://dx.doi.org/10.1128/jb.01443-08.

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ABSTRACT Secretins are bacterial outer membrane proteins that are important for protein export. However, they can also mislocalize and cause stress to the bacterial cell, which is dealt with by the well-conserved phage shock protein (Psp) system in a highly specific manner. Nevertheless, some bacteria have secretins but no Psp system. A notable example is Pseudomonas aeruginosa, a prolific protein secretor with the potential to produce seven different secretins. We were interested in investigating how P. aeruginosa might deal with the potential for secretin-induced stress without a Psp system. Microarray analysis revealed the absence of any transcriptional response to XcpQ secretin overproduction. However, transposon insertions in either rpoN, truB, PA4068, PA4069, or PA0943 rendered P. aeruginosa hypersensitive to XcpQ production. The PA0943 gene was studied further and found to encode a soluble periplasmic protein important for XcpQ localization to the outer membrane. Consistent with this, a PA0943 null mutation reduced the levels of type 2 secretion-dependent proteins in the culture supernatant. Therefore, this work has identified a novel protein required for normal secretin function in P. aeruginosa. Taken together, all of our data suggest that P. aeruginosa lacks a functional equivalent of the Psp stress response system. Rather, null mutations in genes such as PA0943 may cause increased secretin-induced stress to which P. aeruginosa cannot respond. Providing the PA0943 mutant with the ability to respond, in the form of critical Psp proteins from another species, alleviated its secretin sensitivity.

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Schmidberger, Jason W., Robert Schnell i Gunter Schneider. "Structural characterization of substrate and inhibitor binding to farnesyl pyrophosphate synthase fromPseudomonas aeruginosa". Acta Crystallographica Section D Biological Crystallography 71, nr 3 (26.02.2015): 721–31. http://dx.doi.org/10.1107/s1399004715001121.

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Locus PA4043 in the genome ofPseudomonas aeruginosaPAO1 has been annotated as coding for a farnesyl pyrophosphate synthase (FPPS). This open reading frame was cloned and expressed recombinantly inEscherichia coli. The dimeric enzyme shows farnesyl pyrophosphate synthase activity and is strongly inhibited by ibandronate and zoledronate, drugs that are presently in clinical use. The structures of the unliganded enzyme and complexes with the substrate geranyl diphosphate (GPP), the inhibitor ibandronate and two compounds obtained from a differential scanning fluorimetry-based screen of a fragment library were determined by X-ray crystallography to resolutions of better than 2.0 Å. The enzyme shows the typical α-helical fold of farnesyl pyrophosphate synthases. The substrate GPP binds in the S1 substrate site in an open conformation of the enzyme. In the enzyme–ibandronate complex three inhibitor molecules are bound in the active site of the enzyme. One inhibitor molecule occupies the allylic substrate site (S1) of each subunit, as observed in complexes of nitrogen-containing bisphosphonate inhibitors of farnesyl synthases from other species. Two (in subunitA) and one (in subunitB) additional ibandronate molecules are bound in the active site. The structures of the fragment complexes show two molecules bound in a hydrophobic pocket adjacent to the active site. This allosteric pocket, which has previously only been described for FPPS from eukaryotic organisms, is thus also present in enzymes from pathogenic prokaryotes and might be utilized for the design of inhibitors of bacterial FPPS with a different chemical scaffold to the highly charged bisphosphonates, which are less likely to pass bacterial membranes.

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Gordon, Stuart G., Patrick E. Lipps i Richard C. Pratt. "Heritability and Components of Resistance to Cercospora zeae-maydis Derived from Maize Inbred VO613Y". Phytopathology® 96, nr 6 (czerwiec 2006): 593–98. http://dx.doi.org/10.1094/phyto-96-0593.

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Gray leaf spot (GLS), caused by the fungus Cercospora zeae-maydis, is one of the most important foliar diseases of maize. This study was undertaken to estimate heritability of C. zeae-maydis resistance and examine the relationship between previously identified resistance loci and certain components of resistance including incubation period, lesion number, and maximum lesion length. Partially inbred progenies arising from hybridization between maize inbred lines VO613Y (high level of partial resistance) and Pa405 (susceptible) were examined in Ohio and South Africa. Heritability estimates of resistance were calculated based on severity and incubation period values. The range of heritability estimates based on severity was broad, with values ranging from approximately 0.46 to 0.81 (mean = 0.59). Estimates of mean heritability for incubation period were lowest (0.18), indicating that this component would likely be unsuitable for selection of germ plasm intended for deployment in diverse regions. Length of GLS lesions was significantly affected by host genotype, with resistant genotypes having shorter lesions from one site in Ohio during two seasons. Genotype also had a significant effect on incubation period and lesion number; the lower values for these components also were associated with resistant genotypes. The combined action of these resistance components resulted in lower overall disease severity.

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Pires, Gabriela Da Silva, i Luiz Fernando Matos Rocha. "A Construção Concessivo-Comparativa Anteposta Enfática “ATÉ QUE PARA X, Y”: elementos holisticamente empregados para expressar concessividade e comparação". Revista Linguíʃtica 14, nr 1 (30.04.2018): 83. http://dx.doi.org/10.31513/linguistica.2018.v14n1a15351.

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Este trabalho apresenta alguns resultados do estudo da Construção Concessivo-Comparativa Anteposta Enfática (CCCAE), esquematizada como “até que para x, y” e instanciada em ocorrências como “Até que para um iniciante me saí bem”. Seguindo o aporte sociocognitivista da Gramática das Construções (GOLDBERG, 1995, 2006; FILLMORE, LEE-GOLDMAN & RHOMIEUX, 2012), e igualmente ancorados nas abordagens sobre concessividade (KÖNIG, 1985) e comparação linguística (HASEGAWA et al., 2010), buscamos legitimar nosso objeto de estudo como uma Construção Concessivo-Comparativa. Após análise empírica dos dados (286ocorrências coletadas da Web), abordamos alguns aspectos semântico-pragmáticos da CCCAE e destacamos que esta construção apresenta a correferencialidade entre x e y como configuração mais central.

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Endoh, Takayuki, i Joanne N. Engel. "CbpA: a Polarly Localized Novel Cyclic AMP-Binding Protein in Pseudomonas aeruginosa". Journal of Bacteriology 191, nr 23 (2.10.2009): 7193–205. http://dx.doi.org/10.1128/jb.00970-09.

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ABSTRACT In Pseudomonas aeruginosa, cyclic AMP (cAMP) signaling regulates the transcription of hundreds of genes encoding diverse virulence factors, including the type II secretion system (T2SS) and type III secretion system (T3SS) and their associated toxins, type IV pili (TFP), and flagella. Vfr, a cAMP-dependent transcriptional regulator that is homologous to the Escherichia coli catabolite repressor protein, is thought to be the major cAMP-binding protein that regulates these important virulence determinants. Using a bioinformatic approach, we have identified a gene (PA4704) encoding an additional putative cAMP-binding protein in P. aeruginosa PAO1, which we herein refer to as CbpA, for cAMP-binding protein A. Structural modeling predicts that CbpA is composed of a C-terminal cAMP-binding (CAP) domain and an N-terminal degenerate CAP domain and is structurally similar to eukaryotic protein kinase A regulatory subunits. We show that CbpA binds to cAMP-conjugated agarose via its C-terminal CAP domain. Using in vitro trypsin protection assays, we demonstrate that CbpA undergoes a conformational change upon cAMP binding. Reporter gene assays and electrophoresis mobility shift assays defined the cbpA promoter and a Vfr-binding site that are necessary for Vfr-dependent transcription. Although CbpA is highly regulated by Vfr, deletion of cbpA did not affect known Vfr-dependent functions, including the T2SS, the T3SS, flagellum- or TFP-dependent motility, virulence in a mouse model of acute pneumonia, or protein expression profiles. Unexpectedly, CbpA-green fluorescent protein was found to be localized to the flagellated old cell pole in a cAMP-dependent manner. These results suggest that polar localization of CbpA may be important for its function.

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Sugarman, Alan. "Mentalization, insightfulness, and therapeutic action: The importance of mental organization". International Journal of Psychoanalysis 87, nr 4 (sierpień 2006): 965–87. http://dx.doi.org/10.1516/6dgh-0kjt-pa40-rex9.

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Jones, M. W., M. G. Redinbaugh i R. Louie. "The Mdm1 Locus and Maize Resistance to Maize dwarf mosaic virus". Plant Disease 91, nr 2 (luty 2007): 185–90. http://dx.doi.org/10.1094/pdis-91-2-0185.

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Previously, Mdm1, a gene controlling resistance to Maize dwarf mosaic virus (MDMV), was identified in the inbred line Pa405. The gene was tightly linked to the restriction fragment length polymorphism marker umc85 on the short arm of chromosome 6. This chromosomal region is also the location of resistance genes to two other viruses in the family Potyviridae, Sugarcane mosaic virus (SCMV) and Wheat streak mosaic virus (WSMV). A diverse collection of 115 maize inbred lines was evaluated for resistance to MDMV and SCMV, and for MDMV resistance loci on chromosome 6S. Forty-six resistant inbred lines were crossed to three MDMVsusceptible inbred lines to develop F2 populations. The F2 populations were inoculated with MDMV and scored for infection and symptom type. Environmental factors influenced both the rate and type of symptom development. Bulked segregant analysis of each F2 population indicated that, in 42 of 43 MDMV-resistant lines, chromosome 6S markers found in the resistant parent also were present in the bulked resistant but not the susceptible tissue. Markers previously associated with resistance to both SCMV and WSMV on chromosome 3 and to WSMV on chromosome 10 were associated with resistance in nine and seven of the F2 populations, respectively. These data suggest that Mdm1 or closely linked genes on chromosome 6S are associated with MDMV resistance in most germplasm, but that other loci also may affect resistance.

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Gentzel, Irene N., Pierce Paul, Guo-Liang Wang i Erik W. Ohlson. "Effects of Maize Chlorotic Mottle Virus and Potyvirus Resistance on Maize Lethal Necrosis Disease". Phytopathology® 114, nr 2 (1.02.2024): 484–95. http://dx.doi.org/10.1094/phyto-05-23-0171-r.

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Maize lethal necrosis (MLN) is a viral disease caused by host co-infection by maize chlorotic mottle virus (MCMV) and a potyvirus, such as sugarcane mosaic virus (SCMV). The disease is most effectively managed by growing MLN-resistant varieties. However, the relative importance of MCMV and potyvirus resistance in managing this synergistic disease is poorly characterized. In this study, we evaluated the effects of SCMV and/or MCMV resistance on disease, virus titers, and synergism and explored expression patterns of known potyvirus resistance genes TrxH and ABP1. MLN disease was significantly lower in both the MCMV-resistant and SCMV-resistant inbred lines compared with the susceptible control Oh28. Prior to 14 days postinoculation (dpi), MCMV titers in resistant lines N211 and KS23-6 were more than 100,000-fold lower than found in the susceptible Oh28. However, despite no visible symptoms, titer differences between MCMV-resistant and -susceptible lines were negligible by 14 dpi. In contrast, systemic SCMV titers in the potyvirus-resistant line, Pa405, ranged from 130,000-fold to 2 million-fold lower than susceptible Oh28 as disease progressed. Initial TrxH expression was up to 49,000-fold lower in Oh28 compared with other genotypes, whereas expression of ABP1 was up to 4.5-fold lower. Measures of virus synergy indicate that whereas MCMV resistance is effective in early infection, strong potyvirus resistance is critical for reducing synergist effects of co-infection on MCMV titer. These results emphasize the importance of both potyvirus resistance and MCMV resistance in an effective breeding program for MLN management.

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Russo, R., O. Sasso, G. D'Agostino, A. Iacono, G. MattaceRaso, M. Palomba, R. Meli, R. Berni Canani i A. Calignano. "PA40 EFFECTS OF N-(1-CARBAMOYL-2-PHENYL-ETHYL) BUTYRAMIDE, A NEW SYNTHETIC BUTYRATE PRODRUG ON GASTROINTESTINAL TRANSIT TIME MODULATION". Digestive and Liver Disease 42 (październik 2010): S357. http://dx.doi.org/10.1016/s1590-8658(10)60634-2.

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Adage, Tiziana, Francesca del Bene, Francesco Fiorentini, Robert P. Doornbos, Christina Zankl, Michael R. Bartley i Andreas J. Kungl. "PA401, a novel CXCL8-based biologic therapeutic with increased glycosaminoglycan binding, reduces bronchoalveolar lavage neutrophils and systemic inflammatory markers in a murine model of LPS-induced lung inflammation". Cytokine 76, nr 2 (grudzień 2015): 433–41. http://dx.doi.org/10.1016/j.cyto.2015.08.006.

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Wagner, P., S. Salvatore, C. Luini, A. De Paoli, G. Bianchi, A. Salvatoni i L. Nespoli. "PA44 PANCREATITIS SECONDARY TO A DUODENAL HEMATOMA AFTER ENDOSCOPIC SMALL BOWEL BIOPSY IN A BOY WITH POOR FEEDING AND FAILURE TO THRIVE: BE AWARE OF NOONAN SYNDROME". Digestive and Liver Disease 41 (październik 2009): S238. http://dx.doi.org/10.1016/s1590-8658(09)60549-1.

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Wang, Yulu, Liyue Wang, Jian Zhang, Xintong Duan, Yuqi Feng, Shiwei Wang i Lixin Shen. "PA0335, a Gene Encoding Histidinol Phosphate Phosphatase, Mediates Histidine Auxotrophy in Pseudomonas aeruginosa". Applied and Environmental Microbiology 86, nr 5 (20.12.2019). http://dx.doi.org/10.1128/aem.02593-19.

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ABSTRACT The biosynthesis of histidine, a proteinogenic amino acid, has been extensively studied due to its importance in bacterial growth and survival. Histidinol-phosphate phosphatase (Hol-Pase), which is responsible for the penultimate step of histidine biosynthesis, is generally the last enzyme to be characterized in many bacteria because its origin and evolution are more complex compared to other enzymes in histidine biosynthesis. However, none of the enzymes in histidine biosynthesis, including Hol-Pase, have been characterized in Pseudomonas aeruginosa, which is an important opportunistic Gram-negative pathogen that can cause serious human infections. In our previous work, a transposon mutant of P. aeruginosa was found to display a growth defect on glucose-containing minimal solid medium. In this study, we found that the growth defect was due to incomplete histidine auxotrophy caused by PA0335 inactivation. Subsequently, PA0335 was shown to encode Hol-Pase, and its function and enzymatic activity were investigated using genetic and biochemical methods. In addition to PA0335, the roles of 12 other predicted genes involved in histidine biosynthesis in P. aeruginosa were examined. Among them, hisC2 (PA3165), hisH2 (PA3152), and hisF2 (PA3151) were found to be dispensable for histidine synthesis, whereas hisG (PA4449), hisE (PA5067), hisF1 (PA5140), hisB (PA5143), hisI (PA5066), hisC1 (PA4447), and hisA (PA5141) were essential because deletion of each resulted in complete histidine auxotrophy; similar to the case for PA0335, hisH1 (PA5142) or hisD (PA4448) deletion caused incomplete histidine auxotrophy. Taken together, our results outline the histidine synthesis pathway of P. aeruginosa. IMPORTANCE Histidine is a common amino acid in proteins. Because it plays critical roles in bacterial metabolism, its biosynthetic pathway in many bacteria has been elucidated. However, the pathway remains unclear in Pseudomonas aeruginosa, an important opportunistic pathogen in clinical settings; in particular, there is scant knowledge about histidinol-phosphate phosphatase (Hol-Pase), which has a complex origin and evolution. In this study, P. aeruginosa Hol-Pase was identified and characterized. Furthermore, the roles of all other predicted genes involved in histidine biosynthesis were examined. Our results illustrate the histidine synthesis pathway of P. aeruginosa. The knowledge obtained from this study may help in developing strategies to control P. aeruginosa-related infections. In addition, some enzymes of the histidine synthesis pathway from P. aeruginosa might be used as elements of histidine synthetic biology in other industrial microorganisms.

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Fernández-Silva, Arline, Ana L. Juárez-Vázquez, Lilian González-Segura, Javier Andrés Juárez-Díaz i Rosario A. Muñoz-Clares. "The uncharacterized Pseudomonas aeruginosa PA4189 is a novel and efficient aminoacetaldehyde dehydrogenase". Biochemical Journal, 2.02.2023. http://dx.doi.org/10.1042/bcj20220567.

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Neither the Pseudomonas aeruginosa aldehyde dehydrogenase encoded by the PA4189 gene nor its ortholog proteins have been biochemically or structurally characterized and their physiological function is unknown. We cloned the PA4189 gene, obtained the PA4189 recombinant protein, and studied its structure-function relationships. PA4189 is an NAD+-dependent aminoaldehyde dehydrogenase highly efficient with protonated aminoacetaldehyde and 3-aminopropionaldehyde, which are much more preferred to the non-protonated species as indicated by pH studies. Based on the higher activity with aminoacetaldehyde than with 3-aminopropionaldehyde, we propose that aminoacetaldehyde might be the PA4189 physiological substrate. Even though at the physiological pH of P. aeruginosa cells the non-protonated aminoacetaldehyde species will be predominant, and despite the competition of these species with the protonated ones, PA4189 would very efficiently oxidize ACTAL in vivo, producing glycine. To our knowledge, PA4189 is the first reported enzyme that might metabolize ACTAL, which is considered a dead-end metabolite because its consuming reactions are unknown. The PA4189 crystal structure reported here suggested that the charge and size of the active-site residue Glu457, which narrows the aldehyde-entrance tunnel, greatly define the specificity for small positively charged aldehydes, as confirmed by the kinetics of the E457G and E457Q variants. Glu457 and the residues that determine Glu457 conformation inside the active site are conserved in the PA4189 orthologs, which we only found in proteobacteria species. Also is conserved the PA4189 genomic neighborhood, which suggests that PA4189 participates in an uncharacterized metabolic pathway. Our results open the door to future efforts to characterize this pathway.

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Asfew, Kasahun Niguse, Jan Ivens i David Moens. "Temperature dependence of thermophysical properties of carbon/polyamide410 composite". Functional Composite Materials 3, nr 1 (28.11.2022). http://dx.doi.org/10.1186/s42252-022-00036-6.

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AbstractIn this study, the temperature dependence of the carbon/polyamide 410 composite's heat capacity, thermal expansion, density, and thermal conductivity was investigated. The results demonstrated that the specific heat capacity of the C/PA410 composite increases with temperature, with major transitions observed at the glass transition (Tg) and melting (Tm) temperatures. Due to the presence of fibers, the CTE values in the fiber direction of C/PA410 specimens were one order of magnitude smaller than in the transverse direction. The density measurements reveal that as temperature rises, volume increases, causing density to decrease. The heat diffusivity of the C/PA410 composite was measured using the laser flash technique, which was then used to calculate thermal conductivity. The results show that the average thermal conductivity in the fiber direction increases linearly with temperature, while in the transverse direction it increases linearly with temperature up to 50 °C and then becomes constant between 50 °C and 100 °C.

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Zhang, Yong‐Mei, i Charles O. Rock. "Characterization of Pseudomonas aeruginosa DesT (PA4890), a transcriptional factor sensing long‐chain acyl‐CoAs". FASEB Journal 20, nr 4 (marzec 2006). http://dx.doi.org/10.1096/fasebj.20.4.a93-a.

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Stark, Marcel, i Rainer Lauterbach. "Practical Applications of LBO and VC Investments in Recent Crises". Journal of Alternative Investments, 28.02.2022, jai.2021.pa446. http://dx.doi.org/10.3905/jai.2021.pa446.

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Alexander, Carol, i Arben Imeraj. "Practical Applications of The Bitcoin VIX and Its Variance Risk Premium". Journal of Alternative Investments, 28.02.2022, jai.2021.pa447. http://dx.doi.org/10.3905/jai.2021.pa447.

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Brown, Gregory W., Hu Wendy Y. i Jian Zhang. "Practical Applications of The Evolution of Private Equity Fund Value". Journal of Alternative Investments, 28.02.2022, jai.2021.pa448. http://dx.doi.org/10.3905/jai.2021.pa448.

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Parker, Franklin J. "Practical Applications of Achieving Goals While Making an Impact: Balancing Financial Goals with Impact Investing". Practical Applications, 8.12.2021, pa.2021.pa470. http://dx.doi.org/10.3905/pa.2021.pa470.

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