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Artykuły w czasopismach na temat "Osteoblasts"
Kim, Jung Ha, Kabsun Kim, Inyoung Kim, Semun Seong, Jeong-Tae Koh i Nacksung Kim. "The ATF3–OPG Axis Contributes to Bone Formation by Regulating the Differentiation of Osteoclasts, Osteoblasts, and Adipocytes". International Journal of Molecular Sciences 23, nr 7 (23.03.2022): 3500. http://dx.doi.org/10.3390/ijms23073500.
Pełny tekst źródłaBauer, Omri, Amnon Sharir, Ayako Kimura, Shay Hantisteanu, Shu Takeda i Yoram Groner. "Loss of Osteoblast Runx3 Produces Severe Congenital Osteopenia". Molecular and Cellular Biology 35, nr 7 (20.01.2015): 1097–109. http://dx.doi.org/10.1128/mcb.01106-14.
Pełny tekst źródłaPark, Yu-Seong, Hyun-Woo Kim, Jin-Hyeon Hwang, Jung-Young Eom, Dong-Ha Kim, Jinho Park, Hyun-Jin Tae i in. "Plum-Derived Exosome-like Nanovesicles Induce Differentiation of Osteoblasts and Reduction of Osteoclast Activation". Nutrients 15, nr 9 (27.04.2023): 2107. http://dx.doi.org/10.3390/nu15092107.
Pełny tekst źródłaKim, Jung Ha, Kabsun Kim, Inyoung Kim, Semun Seong, Hyun Kook, Kyung Keun Kim, Jeong-Tae Koh i Nacksung Kim. "Bifunctional Role of CrkL during Bone Remodeling". International Journal of Molecular Sciences 22, nr 13 (29.06.2021): 7007. http://dx.doi.org/10.3390/ijms22137007.
Pełny tekst źródłaDucy, P., i G. Karsenty. "Two distinct osteoblast-specific cis-acting elements control expression of a mouse osteocalcin gene." Molecular and Cellular Biology 15, nr 4 (kwiecień 1995): 1858–69. http://dx.doi.org/10.1128/mcb.15.4.1858.
Pełny tekst źródłaMoriishi, Takeshi, Yosuke Kawai, Ryo Fukuyama, Yuki Matsuo, You-Wen He, Haruhiko Akiyama, Izumi Asahina i Toshihisa Komori. "Bcl2l1 Deficiency in Osteoblasts Reduces the Trabecular Bone Due to Enhanced Osteoclastogenesis Likely through Osteoblast Apoptosis". International Journal of Molecular Sciences 24, nr 24 (10.12.2023): 17319. http://dx.doi.org/10.3390/ijms242417319.
Pełny tekst źródłaSutton, Amelia L. M., Xiaoxue Zhang, Diane R. Dowd, Yogendra P. Kharode, Barry S. Komm i Paul N. MacDonald. "Semaphorin 3B Is a 1,25-Dihydroxyvitamin D3-Induced Gene in Osteoblasts that Promotes Osteoclastogenesis and Induces Osteopenia in Mice". Molecular Endocrinology 22, nr 6 (1.06.2008): 1370–81. http://dx.doi.org/10.1210/me.2007-0363.
Pełny tekst źródłaGiuliani, Nicola, Francesca Morandi, Sara Tagliaferri, Mirca Lazzaretti, Sabrina Bonomini, Monica Crugnola, Cristina Mancini i in. "The proteasome inhibitor bortezomib affects osteoblast differentiation in vitro and in vivo in multiple myeloma patients". Blood 110, nr 1 (1.07.2007): 334–38. http://dx.doi.org/10.1182/blood-2006-11-059188.
Pełny tekst źródłaOgata, Naoshi, Hiroshi Kawaguchi, Ung-il Chung, Sanford I. Roth i Gino V. Segre. "Continuous Activation of Gαq in Osteoblasts Results in Osteopenia through Impaired Osteoblast Differentiation". Journal of Biological Chemistry 282, nr 49 (5.09.2007): 35757–64. http://dx.doi.org/10.1074/jbc.m611902200.
Pełny tekst źródłaTaichman, Russell S. "Blood and bone: two tissues whose fates are intertwined to create the hematopoietic stem-cell niche". Blood 105, nr 7 (1.04.2005): 2631–39. http://dx.doi.org/10.1182/blood-2004-06-2480.
Pełny tekst źródłaRozprawy doktorskie na temat "Osteoblasts"
Teixeira, Lucas Novaes 1981. "Estudo da expressão de osteopontina em sistemas de coculturas de células osteoblásticas e epiteliais neoplásicas humanas e seus efeitos sobre o fenótipo neoplásico e a ativação osteoclástica". [s.n.], 2015. http://repositorio.unicamp.br/jspui/handle/REPOSIP/288466.
Pełny tekst źródłaTese (doutorado) - Universidade Estadual de Campinas, Faculdade de Odontologia de Piracicaba
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Resumo: O carcinoma espinocelular (CEC) oral representa a neoplasia maligna mais prevalente das estruturas bucais, podendo invadir o tecido ósseo e promover sua reabsorção em até 56% dos casos. A expressão da proteína matricelular osteopontina (OPN) tem sido relacionada a uma maior agressividade de neoplasias malignas, incluindo o CEC oral. No tecido ósseo, a OPN representa a proteína mais abundante da matriz não colágena, concentrada nas interfaces ósseas, i.e. lâminas limitantes, linhas de cimentação e reversas, sendo essencial para adesão e funções de osteoblastos e osteoclastos. Apesar de no microambiente tumoral a OPN estar associada a um fenótipo neoplásico mais agressivo, ainda não está estabelecido o papel da OPN secretada por osteoblastos sobre células neoplásicas derivadas de CEC oral e o impacto sobre osteoclastos. O presente estudo teve como objetivos avaliar a expressão de OPN em sistemas de coculturas de células osteoblásticas e epiteliais neoplásicas malignas humanas e os efeitos da expressão de OPN secretada por osteoblastos sobre o fenótipo neoplásico in vitro. Adicionalmente, avaliou-se o efeito das coculturas sobre a atividade osteoclástica. Células epiteliais neoplásicas malignas derivadas de CEC oral (SCC9) foram plaqueadas sobre membranas de Transwell®, recobertas ou não por uma camada fina e uniforme de Matrigel, e cocultivadas com células osteoblásticas (SAOS-2) durante seu pico de expressão de OPN (10o dia de cultura). Células SCC9 expostas a culturas SAOS-2 silenciadas para OPN por RNA de interferência (RNAi) e células SCC9 cultivadas isoladamente foram usadas como controles. Após 24 h de cocultivo, células SCC9 foram avaliadas, quantitativamente, para adesão, proliferação, migração e invasão de Matrigel. A atividade de osteoclastos derivados de células monocíticas U-937 foi avaliada, quantitativamente, por meio dos ensaios de reabsorção de fosfato cálcio e de dosagem de citocinas em eluentes obtidos de células SCC9 e SAOS-2 após o cocultivo durante o pico de OPN ou com o seu silenciamento. A análise estatística foi realizada pelo teste não-paramétrico de Kruskal-Wallis (p < 0,05). Os resultados indicaram indução recíproca na expressão de OPN em SAOS-2 e SCC9 em cocultura. A OPN secretada por células SAOS-2 afetou o fenótipo de culturas SCC9, promovendo a adesão e a proliferação celulares e a invasão de Matrigel, a qual também estava aumentada, mas em menor intensidade, com o silenciamento para OPN. A migração celular não foi afetada. O cocultivo com SAOS-2, principalmente durante o pico de OPN, resultou na sobre-expressão das citocinas IL 6 e IL 8 pelas células SCC9, aumentando a capacidade de células osteoclásticas em reabsorver fosfato de cálcio. Conjuntamente, esses resultados sugerem que a OPN derivada de osteoblastos afeta as interações entre células epiteliais neoplásicas malignas, osteoblastos e osteoclastos, possivelmente contribuindo para a progressão de lesões ósseas do CEC oral
Abstract: The oral squamous cell carcinoma (OSCC) is the most prevalent malignant neoplasm of the oral structures. It may invade bone in up to 56% of the cases and promote osteoclast-mediated bone extracellular matrix (ECM) resorption. Expression of the matricellular protein osteopontin (OPN) in malignant neoplasms, including OSCC, has been positively correlated with aggressive tumor behavior. OPN is the most abundant non collagenous ECM protein in bone, where it preferentially accumulates at interfaces, including cement lines, laminae limitantes and reversal lines, being essential for the adhesion and function of osteoblasts and osteoclasts. Despite the importance attributed to OPN in the tumor microenvironment, indicative of more aggressive neoplastic phenotypes, the effects of osteoblast-derived OPN on OSCC cells and on OSCC-induced osteoclast activity are still not fully understood. The present in vitro study aimed to evaluate temporal expression of OPN in cocultures of human osteoblastic cells and malignant neoplastic epithelial cells and the effects of osteoblast-derived OPN on the neoplastic cell phenotype. Additionally, the effects of cocultures on osteoclastic activity were evaluated. Human OSCC-derived epithelial cells (SCC9 cell line) were plated on Transwell® membranes coated or not by a thin uniform layer of Matrigel and cocultured with human osteoblastic cells (SAOS-2 cell line) during its peak of OPN expression (day 10 of SAOS-2 culture). SCC9 cells exposed to OPN-silenced SAOS-2 cultures by means of interference RNA and SCC9 cells cultured alone were used as controls. At 24 h of coculture, SCC9 cells were quantitatively evaluated for cell adhesion, proliferation, migration and invasion of Matrigel. The impact of coculturing SCC9 and SAOS-2 cells either during the OPN peak expression or under the silencing of OPN was quantitatively evaluated in terms of calcium phosphate resorption by U-937-derived osteoclastic cells and expression of cytokines in the culture medium by ELISA assay. The statistical analyses were carried out using the non-parametric Kruskal-Wallis test (p < 0.05). The results showed a reciprocal induction of SAOS-2 and SCC9 cells in terms of OPN expression over the coculture interval. SAOS-2-secreted OPN altered the SCC9 cell phenotype, leading to enhanced cell adhesion and proliferation and higher Matrigel invasion, which was also enhanced, but to a lesser degree, by SAOS-2 cultures silenced for OPN. Cell migration was not affected. Cocultures with SAOS-2, mainly during the peak expression of OPN, resulted in overexpression of IL 6 and IL 8 by SCC9 cells, which corresponded with an enhanced resorptive capacity of osteoclastic cells. Taken together, the results suggest that osteoblast-derived OPN affects the interactions among malignant neoplastic epithelial cells, osteoblasts and osteoclasts, likely contributing to the progression of bone lesions in OSCC
Doutorado
Patologia
Doutor em Estomatopatologia
Prele, Cecilia Marie Antoinette. "Vesicular trafficking in osteoblasts". Thesis, University College London (University of London), 2001. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.271058.
Pełny tekst źródłaBond, Alistair. "Purinergic signalling in osteoblasts". Thesis, University of Liverpool, 2012. http://livrepository.liverpool.ac.uk/11293/.
Pełny tekst źródłaVasconcelos, Daniel Fernando Pereira. "Efeito da administração intermitente do fragmento 1-34 do hormonio paratireoideo em defeito fenestrado na mandibula de ratos : analise histomorfometrica". [s.n.], 2008. http://repositorio.unicamp.br/jspui/handle/REPOSIP/288498.
Pełny tekst źródłaTese (doutorado) - Universidade Estadual de Campinas, Faculdade de Odontologia de Piracicaba
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Resumo: A administração intermitente de hormônio paratireóideo (PTH) é capaz de promover anabolismo ósseo, favorecendo a neoformação óssea em condições como osteoporose e reparo de fraturas. Sabendo-se que tanto os tecidos ósseos mandibulares e alveolares como os tecidos periodontais podem responder à administração de PTH de forma intermitente, o objetivo deste estudo foi avaliar histomorfometricamente o efeito da administração de PTH (1-34) em um modelo de defeito periodontal, do tipo fenestrado, na mandíbula de ratos. Foram utilizados neste estudo 32 ratos Wistar machos, com o objetivo de expor a região vestibular da raiz distal do primeiro molar inferior direito. A criação do defeito possibilitou a remoção parcial de ligamento periodontal e do cemento dentário. Após a cirurgia os animais foram separados aleatoriamente em 4 grupos (n=8): Grupo C14: administração intermitente (3 vezes por semana) do veículo de diluição do PTH por 14 dias; Grupo P14: administração intermitente de PTH (1-34) por 14 dias; Grupo C21: injeções intermitentes do veículo de diluição do PTH por 21 dias; Grupo P21: injeções intermitentes de PTH por 21 dias. Os animais foram mortos e preparados para avaliação histomorfométrica dos seguintes parâmetros: I - extensão inicial do defeito, II - extensão do defeito remanescente, III - área do defeito ósseo remanescente, IV - densidade do osso neoformado, V - área do calo formado e marcação para TRAP, VI - área do cemento neoformado, VII - análise do retardo óptico (birrefringência) do ligamento periodontal reinserido sobre a raiz operada. A análise intergrupo demonstrou que os defeitos tinham tamanhos similares inicialmente e que a administração de PTH reduziu significativamente (p<0,05) a extensão e a área do defeito remanescente. Além disso, o PTH aumentou significativamente (p<0,05) a densidade do osso neoformado, a área do calo, o número de osteoclastos presentes no calo formado, a área de cemento neoformado e a birrefringência do ligamento periodontal reinserido. Conclui-se que a administração intermitente de PTH (1-34), em modelo experimental de reparo em de ratos, pode influenciar positivamente o processo de reparação óssea e periodontal.
Abstract: The intermittent PTH administration is known to contribute to bone formation in cases of osteoporosis and bone fractures. Also, it has been reported to reduce periodontitis-related bone loss. The aim of this study was to evaluate the effect of anabolic PTH on periodontal repair and mandibular bone defect in rats. Fenestration defects were created unilaterally at the buccal surface of distal roots of lower first molars in Wistar rats (n=32), and both periodontal ligament and cementum were removed. Animals were then assigned to 4 groups (n=8): 1) C14 - placebo administration for 14 days; 2) P14 - PTH administration for 14 days; 3) C21 - placebo administration for 21 days; and 4) P21 - PTH administration for 21 days. All groups were treated intermittently (3 times a week). All animals were killed and prepared to histomorphometric analysis considering the following parameters: I - extension of the initial defect; II - extension of the remaining defect; III - area of the remaining defect; IV - density of neoformed bone; V - total callus area and staining for tartrate-resistant acidic phosphatase (TRAP); VI - area of the neoformed cementum; VII - polarized light microscopic analysis of root periodontal ligament reattachment. Intergroup analysis showed that the bone defects were initially similar in size (p>0.05). The intermittent PTH administration decreased (p<0.05) both extension and area of the remaining defect, and increased (p<0.05) the neoformed bone density and the total callus area. An increase in TRAP-positive cells was observed in the PTH treated groups (p<0.05). The area of the neoformed cementum and reattachment of the periodontal ligament were also observed to increase concerning PTH-treated groups (p<0.05). Data analysis suggests that the intermittent PTH administration might contribute to bone and periodontal repair in rats.
Doutorado
Histologia e Embriologia
Doutor em Biologia Buco-Dental
Lage, Thais Claudino. "Análise in vitro da citotoxicidade em osteoblastos de dispositivos poliméricos incorporados com antimicrobianos para uso local". Universidade de São Paulo, 2017. http://www.teses.usp.br/teses/disponiveis/23/23147/tde-27112017-154525/.
Pełny tekst źródłaOsteoblasts are mesenquima originated cells, which are involved in the bone formation. These cells may suffer alterations due to traumas, interventions and infeccions. The infections can be minimized by the handling of antimicrobials. Poly (L-lactide) or PLLA is a synthetic polymer known for its biocompatibility and absorption, which can be used as a local pharmacological releaser, as an alternative to the systemic antimicrobial therapy. This polymer also can be frequently used as a supporting structure to cellular matrix in the bone tissue engineering as it can be used for support in repair and regeneration. The particle incorporation in this polymer can create side effects, therefore, we need to certificate that the polymeric device incorporated with antimicrobials are not cytotoxic. Proposition: Analyse the structure and cytotoxicity in osteoblasts of PLLA polymeric devices associated with antimicrobials, being them: Amoxicillin, Azithromycin, Clindamycin and Metronidazole. Methods: For this study 270 polymerical devices were manufactured with 6mm diameter of PLLA with a 20% antimicrobials incorporation of Amoxicillin (AM), Azithromycin (AZ), Clindamycin (CL) and Metronidazole (ME) that have been produced through two methods: eletrospinning (mesh) or casting (film). Afterwards a MTT cytotoxicity test was made over the periods of 24, 48 and 72 hours of experiment. To make a structural analysis of the device a macroscopic analysis was performed through photographs and microscopic imaging with scanning electron microscope (SEM). Results: The cytotoxicity reaction exhibited that meshes and films incorporated with antimicrobials are comparable with the osteoblasts culture, indicating that there was no cytotoxicity in any moment (p < 0.05). In the phothograph we could observe that the devices showed a similar coloration among the meshes and different coloration for the films depending on the incorporated antimicrobial. The SEM analysis displayed a difference in the surface appearance of the films. The AM films displayed an irregular and porous appearance, meanwhile, AZ looked smooth with few grains, the CL and ME have rough surfaces and PLLA presents smooth surfaces. As for the meshes, we noticed that all the samples had microfibers and pores that mimic the extracellular matrix, differing only in the thickness of the fibers. Osteoblasts were present in all films but AM did not induce proliferation, with only isolated cells emerging. In meshes osteoblasts were only found in AM, ME and PLLA. Conclusion: Polymeric devices made with PLLA incorporated with antimicrobials can be used in bone repair and regeneration given that they did not offer cytotoxicity for osteoblasts.
Valdivia, Maria Alejandra Medina. "Cultura e caracterização de células da granulação óssea in vitro: efeitos proliferativos estimulados por diferentes biomateriais". Universidade de São Paulo, 2013. http://www.teses.usp.br/teses/disponiveis/25/25146/tde-03092013-162521/.
Pełny tekst źródłaThe aim of this study was to establish primary culture of cells derived from human bone granulation tissue (GO) in order to determine its growth pattern in vitro and the biological effects of three absorbable collagen membranes (BioGide®, GenDerm®, CollaTape®) in human gingival fibroblasts (FGH) and human bone granulation (GO) cell cultures. Samples of bone tissue present at healing sockets of two systemically healthy adults with indication of periodontal regenerative therapy by the newly forming bone were collected. Immediately after, samples were transported to the laboratory of cell culture to the establishment of primary cultures. Cells were cultivated in humid atmosphere with 95% CO2 at 37oC. Cells growth pattern were determined by counting of viable cells. After characterization of growth pattern, samples were characterized according to alkaline phosphatase activity and mineralization detected by alizarin red. Afterwards, the effects of three different types of collagen membranes on GO and FGH cells were investigated by MTT test. Samples were divided into eight groups: (1) FGH cells in DMEM (C-FGH); (2) FGH in DMEM conditioned by GenDerm® membrane (GD-FGH); (3) FGH in DMEM conditioned with BioGide® (BG-FGH); (4) FGH in DMEM conditioned by CollaTape® (CT-FGH); (5) GO cells in DMEM (C-GO); (6) GO cells in DMEM conditioned by GenDerm® (GD-GO); (7) GO cells in DMEM conditioned by BioGide® (BG-GO); (8) GO cells in DMEM conditioned by CollaTape® (CT-GO). Cell proliferation test showed a significant (p< 0.05; ANOVA for repeated measures) increase in the number of vital cells present in the culture at days 3 (90.8%), 5 (132.50%), 7 (137.50%) and 10 (227.50%) compared to control (dia 0). It was observed alkaline phosphatase activity and mineralization in vitro. There was an increase in the number of FGH and GO viable cells at all groups (p< 0.05; ANOVA for repeated measures). Greater proliferative effect at FGH and GO cells at GD and CT groups, with significant differences between groups (p< 0.05; Mann Whitney) only at 96 hs. Two of the collagen membranes tested exerted greater late proliferative effects on osteoblasts, suggesting its efficacy in the regeneration of periodontal tissues.
Mello, Daphne de Camargo Reis. "Interações biológicas e microbiológicas da liga Ti-35Nb-7Zr oxidadas e não oxidadas : estudo in vitro /". São José dos Campos, 2017. http://hdl.handle.net/11449/150556.
Pełny tekst źródłaBanca: Luciane Dias de Oliveira
Banca: Sandra Giacomin Schneider
Resumo: O objetivo neste estudo foi avaliar in vitro a influência da liga Ti-35Nb-7Zr e de seus elementos básicos, submetidos ou não ao processo de oxidação, na atividade de osteoblastos e na formação de biofilmes monotípicos. As amostras foram confeccionadas com diferentes materiais: a) titânio puro (Ti - controle); b) liga Ti-35Nb-7Zr (L); c) Nb (nióbio); d) Zr (zircônio); e) Ti oxidado (TiO); f) liga Ti-35Nb-7Zr oxidada (LO); g) Nb oxidado (NbO); h) Zr oxidado(ZrO). Previamente ao estudo in vitro, todas as amostras foram caracterizadas por microscopia eletrônica de varredura (MEV) e espectroscopia de dispersão de energia (EDS) para observar a topografia de superfície e a presença dos elementos básicos. Células mesenquimais obtidas de fêmures de rato, após diferenciação em osteoblastos foram cultivadas sobre as amostras. Após períodos pré-determinados, foram realizados os testes de citotoxicidade, atividade de fosfatase alcalina, produção de proteína total, formação e quantificação dos nódulos de mineralização adesão e proliferação celular. Para análise da formação dos biofilmes monotípicos, suspensões padronizadas (106 céls/mL) com micro-organismos de S. aureus, S. mutans, P. aeruginosa e C. albicans foram cultivados por 24 h sobre as amostras e submetidos ao teste de MTT. Todas as amostras permitiram o espraiamento celular. O Nb e Zr exibiram uma adesão celular mais madura com prolongamentos celulares evidenciados. O Ti e a L apresentaram maior viabilidade celular sendo estatísti... (Resumo completo, clicar acesso eletrônico abaixo)
Abstract: The objective of this study was to evaluate in vitro the influence of the Ti-35Nb- 7Zr alloy and its basic elements, whether or not subjected to the oxidation process, on the osteoblast activity and on the formation of monotypic biofilms. The samples were made with different materials: a) pure titanium (Ti - control); B) Ti-35Nb-7Zr (A) alloy; C) Nb (niobium); D) Zr (zirconium); E) Oxidized Ti (TiO); F) oxidized Ti-35Nb-7Zr (AO); G) Nb oxidized (NbO); H) Oxidized Zr (ZrO). Prior to the in vitro study, all samples were characterized by scanning electron microscopy (SEM) and energy dispersive spectroscopy (EDS) to observe the surface topography and the presence of the basic elements. Mesenchymal cells obtained from mouse femurs after differentiation into osteoblasts were cultured in the samples. After pre-determined periods, cytotoxicity tests, alkaline phosphatase activity, total protein production, formation and quantification of the mineralization nodules, adhesion and cell proliferation were performed. To analyze the formation of monotypic biofilms, standardized suspensions (106 cells / ml) with S. aureus, S. mutans, P. aeruginosa and C. albicans microorganisms were cultured for 24 h on the samples and submitted to the MTT test. All samples allowed for cell spreading. Nb and Zr exhibited more mature cell adhesion with evidenced cell extensions. The Ti and A presented greater cell viability being statistically different from the others (p <0.05). ZrO presented lower viability exhibiting statistical difference with Ti and A. The NbO expressed the highest amount of total protein with a statistical difference of L, Zr, and AO (p <0.05) while Zr showed the lowest result with a statistical difference of Ti, Nb, TiO, NbO, and ZrO. In the quantification of the alkaline phosphatase activity, the Zr presented the best result and was statistically different from all the others ..... (Resumo completo, clicar acesso eletrônico abaixo)
Mestre
Bomfim, Fernando Russo Costa do [UNIFESP]. "Laser de baixa intensidade na expressão de genes ligados a mineralização óssea e ao cálcio em cultura de osteoblastos adultos". Universidade Federal de São Paulo (UNIFESP), 2014. http://repositorio.unifesp.br/handle/11600/23253.
Pełny tekst źródłaCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
A reducao ou minimizacao das consequencias das fraturas osseas e a regeneracao tecidual e objetivo de recursos clinicos e terapeuticos. O tecido osseo tem funcao mecanica, de deposito mineral e hematopoietica sendo composto por tres tipos celulares, osteoblastos, osteocitos e osteoclastos. Diversas substancias sao secretadas pelos osteoblastos, entre elas o calcio, que tem papel fundamental na homeostase celular e na producao do tecido osseo mineralizado e em cujo fluxo estao envolvidos os genes S100A6, PMCA1b e a osteocalcina ligados ao processo transporte e de mineralizacao. O objetivo deste trabalho foi avaliar os efeitos do laser de baixa intensidade, no que concerne a sinalizacao de calcio e mineralizacao ossea, na expressao dos genes S100A6, PMCA1b e osteocalcina em celulas osteoblasticas adultas. Trinta fragmentos mediais de femures com aproximadamente 5mm foram coletados cirurgicamente de ratos Wistar e distribuidos em dois grupos A (n=15, laser) e B (n=15, sem laser). Os fragmentos foram digeridos mecanica e enzimaticamente com colageno tipo II. Apos sete dias de cultura o grupo A foi irradiado com laser de baixa intensidade de Arseneto de Galio e Aluminio λ=808nm, 250mW de potencia nominal, densidade de potencia de 200mW/cm2, densidade de energia de 2000mJ/cm2, dose de energia de 2J/cm2, diametro de feixe de 0,02mm, tempo de 5s em 1 ponto de aplicacao durante 6 dias. Em seguida, o RNA foi extraido, quantificado, submetido a sintese de cDNA e realizada quantificacao de Ct comparativo pela Reacao em Cadeia da Polimerase em Tempo Real. Os valores foram submetidos a analise estatistica de teste Mann-Whitney com p<0,05. A analise em relacao a quantidade de RNA dos grupos A (mediana 3,80) e B (mediana 3,80) e os valores das medianas de ΔCt dos tres genes, S100A6 (A=2,22 e B=2,89), osteocalcina (A=4,06 e B=3,45) e PMCA1b (A=1,85 e B=4,16) nao mostraram diferencas significativas. O laser de baixa intensidade em osteoblastos adultos nao alterou a expressao dos genes S100A6, PMCA1b e osteocalcina descartando a relacao, no periodo estudado, destes genes com a mineralizacao e regeneracao ossea
The aim of clinical and therapeutic features is to reduce or minimize the consequences of fractures and bone regeneration. The bone has mechanical, mineral deposit and hematopoietic functions due to three types of cells, osteoblasts, osteocytes and osteoclasts. Several substances are secreted by osteoblasts, including calcium, which plays a fundamental role in cellular homeostasis and production of mineralized bone tissue and in whose flow are involved the S100A6, PMCA1b and osteocalcin genes related to the transport and mineralization process. This study was designed to evaluate the effects of low-level laser, when it comes to calcium signaling and bone mineralization, in S100A6, PMCA1b and osteocalcin genes expression in adult osteoblast cells. Thirty medial femoral fragments with approximately 5mm were surgically collected from Wistar rats and distributed into two groups A (n=15, laser) and B (n=15 without laser). The fragments were digested mechanical and enzymatically with type II collagen. After seven days of culture group A was irradiated with low intensity Gallium-Aluminum-Arsenide laser λ=808nm, 250mW nominal power, power density 200mW/cm2, energy density 2000mJ/cm2 dose of energy 2J/cm2 beam diameter 0,02 mm, length of 5s in one application point for 6 days. Then, RNA was extracted, quantified, underwent to cDNA synthesis and quantification performed by the comparative Ct by Real Time Polymerase Chain Reaction. The values were underwent to statistical analysis by Mann-Whitney test with p<0,05. The analysis for the amount of RNA of group A (median 3,80) and B (median 3,80) and ΔCt median values of the three genes, S100A6 (A=2.22 and B=2.89), osteocalcin (A=4.06 and B=3.45) and PMCA1b (A=1.85 and B=4.16) showed no significant differences. Low-level laser irradiation in adult osteoblasts did not modify the expression of S100A6, PMCA1b and osteocalcin genes discarding the relationship of these genes with the mineralization and bone regeneration in the studied period.
BV UNIFESP: Teses e dissertações
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Pełny tekst źródłaHempel, Ute, Carolin Preissler, Sarah Vogel, Stephanie Möller, Vera Hintze, Jana Becher, Matthias Schnabelrauch, Martina Rauner, Lorenz C. Hofbauer i Peter Dieter. "Artificial Extracellular Matrices with Oversulfated Glycosaminoglycan Derivatives Promote the Differentiation of Osteoblast-Precursor Cells and Premature Osteoblasts". Saechsische Landesbibliothek- Staats- und Universitaetsbibliothek Dresden, 2015. http://nbn-resolving.de/urn:nbn:de:bsz:14-qucosa-165309.
Pełny tekst źródłaKsiążki na temat "Osteoblasts"
Bland, Rosemary. Studies of thyroid hormone action in osteoblasts. Birmingham: University of Birmingham, 1996.
Znajdź pełny tekst źródłaMakinistoglu, Munevver. HDAC4 Integrate PTH and Sympathetic Signaling In Osteoblasts. [New York, N.Y.?]: [publisher not identified], 2014.
Znajdź pełny tekst źródłaKung, Vanessa. Estrogen related receptor [alpha], ERR[alpha], regulation of target genes in osteoblasts. Ottawa: National Library of Canada, 2003.
Znajdź pełny tekst źródłaCompston, Juliet. Osteoporosis and bone biology: The state of the art. London: International Medical Press, 2000.
Znajdź pełny tekst źródłaShelton, Richard Michael. The effect of substratum charge on the interfacial behaviour of osteoblasts in vitro. Birmingham: University of Birmingham, 1989.
Znajdź pełny tekst źródłaMayeenuddin, Linnia H. Modulation of PTH-mediated signal transduction in osteoblasts by factors regulating cellular growth. Ottawa: National Library of Canada = Bibliothèque nationale du Canada, 1999.
Znajdź pełny tekst źródłaNational Institute on Aging/National Institute of Dental Research Workshop on Human Models of Skeletal Aging (1994 Washington, D.C.). National Institute on Aging/National Institute of Dental Research Workshop on Human Models of Skeletal Aging: Washington, DC, March 1-2, 1994. Redaktorzy Robey Pamela Gehron 1952-, Sherman Sherry, National Institute of Dental Research (U.S.) i National Institute on Aging. New York, NY: Springer International, 1995.
Znajdź pełny tekst źródłaWalker, Lesley Margaret. The effect of mechanical and hormonal stimuli on femur derived osteoblasts; intracellular calcium fluxes and calcium channels. Birmingham: University of Birmingham, 1998.
Znajdź pełny tekst źródłaCrothers, Kristina A. Effects of Tamoxifen on Prostaglandin E2 induced insulin-like growth factor I expression in fetal rat osteoblasts. [New Haven, Conn: s.n.], 1997.
Znajdź pełny tekst źródłaDavid, Evered, Harnett Sara i Ciba Foundation, red. Cell and molecular biology of vertebrate hard tissues. Chichester, UK: Wiley, 1988.
Znajdź pełny tekst źródłaCzęści książek na temat "Osteoblasts"
Rhodes, Steven D. "Osteoblasts". W Encyclopedia of Systems Biology, 1616–17. New York, NY: Springer New York, 2013. http://dx.doi.org/10.1007/978-1-4419-9863-7_881.
Pełny tekst źródłaBaak, Marleen A., Bernard Gutin, Kim A. Krawczewski Carhuatanta, Stephen C. Woods, Heinz W. Harbach, Megan M. Wenner, Nina S. Stachenfeld i in. "Osteoblasts". W Encyclopedia of Exercise Medicine in Health and Disease, 671. Berlin, Heidelberg: Springer Berlin Heidelberg, 2012. http://dx.doi.org/10.1007/978-3-540-29807-6_2793.
Pełny tekst źródłaPavelka, Margit, i Jürgen Roth. "Osteoblasts and Osteocytes". W Functional Ultrastructure, 296–97. Vienna: Springer Vienna, 2010. http://dx.doi.org/10.1007/978-3-211-99390-3_152.
Pełny tekst źródłaMartin, T. J., D. M. Findlay, J. K. Heath i K. W. Ng. "Osteoblasts: Differentiation and Function". W Physiology and Pharmacology of Bone, 149–83. Berlin, Heidelberg: Springer Berlin Heidelberg, 1993. http://dx.doi.org/10.1007/978-3-642-77991-6_4.
Pełny tekst źródłaFranceschi, Renny T., Chunxi Ge i Christopher G. Wilson. "Osteoblasts of Craniofacial Bone". W Mineralized Tissues in Oral and Craniofacial Science, 43–57. West Sussex, UK: John Wiley & Sons, Inc.,, 2013. http://dx.doi.org/10.1002/9781118704868.ch6.
Pełny tekst źródłaPerpétuo, Inês P., Lucie E. Bourne i Isabel R. Orriss. "Isolation and Generation of Osteoblasts". W Methods in Molecular Biology, 21–38. New York, NY: Springer New York, 2019. http://dx.doi.org/10.1007/978-1-4939-8997-3_2.
Pełny tekst źródłaYoshida, Takashi, Yuki Miyajima i Minoru Wakamori. "Mechanosensitive TRP channels in osteoblasts". W Interface Oral Health Science 2009, 205–6. Tokyo: Springer Japan, 2010. http://dx.doi.org/10.1007/978-4-431-99644-6_49.
Pełny tekst źródłaKrauze, Michal T., i G. David Roodman. "Bortezomib and Osteoclasts and Osteoblasts". W Bortezomib in the Treatment of Multiple Myeloma, 43–52. Basel: Springer Basel, 2010. http://dx.doi.org/10.1007/978-3-7643-8948-2_3.
Pełny tekst źródłaHughes-Fulford, Millie. "Growth and Repair of Mineralized Osteoblasts". W Spinal Instability, 3–19. New York, NY: Springer New York, 1993. http://dx.doi.org/10.1007/978-1-4613-9326-9_1.
Pełny tekst źródłaGartland, Alison, Robin M. H. Rumney, Jane P. Dillon i James A. Gallagher. "Isolation and Culture of Human Osteoblasts". W Methods in Molecular Biology, 337–55. Totowa, NJ: Humana Press, 2011. http://dx.doi.org/10.1007/978-1-61779-367-7_22.
Pełny tekst źródłaStreszczenia konferencji na temat "Osteoblasts"
Li, K., Y. Xie, M. You, L. Huang i X. Zheng. "Preparation and In Vitro Evaluation of Plasma Sprayed Cerium Oxide Coatings". W ITSC 2016, redaktorzy A. Agarwal, G. Bolelli, A. Concustell, Y. C. Lau, A. McDonald, F. L. Toma, E. Turunen i C. A. Widener. DVS Media GmbH, 2016. http://dx.doi.org/10.31399/asm.cp.itsc2016p0730.
Pełny tekst źródłaTuan, Rocky S. "Functional Analysis of Bone-Biomaterial Interface". W ASME 2000 International Mechanical Engineering Congress and Exposition. American Society of Mechanical Engineers, 2000. http://dx.doi.org/10.1115/imece2000-2675.
Pełny tekst źródłaCox, Lieke G. E., Corrinus C. van Donkelaar, Bert van Rietbergen, Rik Huiskes i Keita Ito. "Osteocytes in Bone Can Regulate the Turnover of Adjacent Mineralized Growth Plate Cartilage". W ASME 2009 Summer Bioengineering Conference. American Society of Mechanical Engineers, 2009. http://dx.doi.org/10.1115/sbc2009-206251.
Pełny tekst źródłaCoughlin, Thomas R., Matthew Haugh, Muriel Voisin, Evelyn Birmingham, Laoise M. McNamara i Glen L. Niebur. "Primary Cilia Knockdown Reduces the Number of Stromal Cells in Three Dimensional Ex Vivo Culture". W ASME 2013 Summer Bioengineering Conference. American Society of Mechanical Engineers, 2013. http://dx.doi.org/10.1115/sbc2013-14723.
Pełny tekst źródłaLiu, X., i C. Ding. "Cytocompatibility of Plasma Sprayed Bioceramic Coatings". W ITSC2005, redaktor E. Lugscheider. Verlag für Schweißen und verwandte Verfahren DVS-Verlag GmbH, 2005. http://dx.doi.org/10.31399/asm.cp.itsc2005p0600.
Pełny tekst źródłaLu, X. Lucas, Bo Huo, Andrew D. Baik i X. Edward Guo. "Calcium Signaling in Bone Cell Networks Induced by Fluid Flow". W ASME 2009 Summer Bioengineering Conference. American Society of Mechanical Engineers, 2009. http://dx.doi.org/10.1115/sbc2009-206043.
Pełny tekst źródłaHoriguchi, Atsushi, i Toshihiko Shiraishi. "Study on a Cell Mechanosensing System by Measuring Structural Deformation and Biochemical Response". W ASME 2015 International Mechanical Engineering Congress and Exposition. American Society of Mechanical Engineers, 2015. http://dx.doi.org/10.1115/imece2015-51456.
Pełny tekst źródłaShiraishi, Toshihiko, Akinori Ishii i Shin Morishita. "Effects of Mechanical Vibration on Multilayering of Cultured Osteoblasts". W ASME 2014 International Mechanical Engineering Congress and Exposition. American Society of Mechanical Engineers, 2014. http://dx.doi.org/10.1115/imece2014-37731.
Pełny tekst źródłaSong, Wang, Yang Yueqin i Chen Guoqing. "Effect of mechanical stress on formation of osteoblast in vitro — A new way to culture osteoblasts". W 2009 ISECS International Colloquium on Computing, Communication, Control, and Management (CCCM). IEEE, 2009. http://dx.doi.org/10.1109/cccm.2009.5267860.
Pełny tekst źródłaUemura, Toshimasa, Atsuko Nemoto, Jing An, Yin-kun Liu, Takafumi Yoshikawa, Hajime Ohgushi, Yoshinori Kuboki, Takashi Ushida i Tetsuya Tateishi. "OSTEOPONTIN INVOLVEMENT IN OSTEOBLAST DIFFERENTIATION AND ITS EFFECT ON IN VIVO OSTEOGENIC POTENTIAL OF BONE MARROW DERIVED OSTEOBLASTS". W Proceedings of the 12th International Symposium on Ceramics in Medicine. WORLD SCIENTIFIC, 1999. http://dx.doi.org/10.1142/9789814291064_0064.
Pełny tekst źródłaRaporty organizacyjne na temat "Osteoblasts"
Gerstenfeld, Louis C. Mechanisms of Mechano-Transduction Within Osteoblasts. Fort Belvoir, VA: Defense Technical Information Center, wrzesień 2001. http://dx.doi.org/10.21236/ada415975.
Pełny tekst źródłaGerstenfeld, Louis C. Mechanisms of Mechano-Transduction within Osteoblasts. Fort Belvoir, VA: Defense Technical Information Center, wrzesień 2000. http://dx.doi.org/10.21236/ada391000.
Pełny tekst źródłaGerstenfeld, Louis. Mechanisms of Mechano-Transduction within Osteoblasts. Fort Belvoir, VA: Defense Technical Information Center, wrzesień 1999. http://dx.doi.org/10.21236/ada376489.
Pełny tekst źródłaVélez, Rómulo Andrés, Alejandro Fereño Caceres, Wilson Daniel Bravo Torres, Daniela Astudillo Rubio i Jacinto José Alvarado Cordero. Primary stability with the osseodensification drilling technique for dental implants in low density bone in humans: a systematic review. INPLASY - International Platform of Registered Systematic Review and Meta-analysis Protocols, wrzesień 2022. http://dx.doi.org/10.37766/inplasy2022.9.0066.
Pełny tekst źródłaMercer, Robyn R. Breast Cancer Metastasis to Bone Affects Osteoblast Differentiation. Fort Belvoir, VA: Defense Technical Information Center, maj 2004. http://dx.doi.org/10.21236/ada426273.
Pełny tekst źródłaMercer, Robyn R., i Andrea M. Mastro. Breast Cancer Metastasis to Bone Affects Osteoblast Differentiation. Fort Belvoir, VA: Defense Technical Information Center, maj 2003. http://dx.doi.org/10.21236/ada416623.
Pełny tekst źródłaKaraplis, Andrew. Osteoblast-Derived PTHRP and Breast Cancer Bone Metastasis. Fort Belvoir, VA: Defense Technical Information Center, listopad 2004. http://dx.doi.org/10.21236/ada625302.
Pełny tekst źródłaNavone, Nora M. Osteoblast-Prostate Cancer Cell Interaction in Prostate Cancer Bone. Fort Belvoir, VA: Defense Technical Information Center, luty 2000. http://dx.doi.org/10.21236/ada391088.
Pełny tekst źródłaParanavitana, Chrysanthi. In Vitro Osteoblast Model for Bone Wound Infections and Antimicrobial Therapy. Fort Belvoir, VA: Defense Technical Information Center, styczeń 2013. http://dx.doi.org/10.21236/ada608594.
Pełny tekst źródłaSanders, Jennifer L. Actions of Tamoxifen and Estrogen on Osteoblast Protein Kinase C Expression. Fort Belvoir, VA: Defense Technical Information Center, lipiec 1995. http://dx.doi.org/10.21236/ada306529.
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