Rozprawy doktorskie na temat „OMD Protocol”

ITC/USA 2008 Conference Proceedings / The Forty-Fourth Annual International Telemetering Conference and Technical Exhibition / October 27-30, 2008 / Town and Country Resort & Convention Center, San Diego, California
As satellite ground systems migrate toward network-centric, distributed architectures, controlling remote ground equipment becomes a central issue. While many protocols and approaches exist that address remote control and status, there is little agreement on a common solution. Device vendors and system integrators commonly find themselves integrating multiple protocols to meet a wide range of requirements. Technologies change and new protocols evolve that result in yet more options to be considered. However, the fundamental aspects of device control remain constant. The Ground Equipment Monitoring Service (GEMS) seeks to define a standard model for device control independent of the underlying protocols and technologies. Using this approach, a wide range of protocols can be mapped to the GEMS model. Systems using different protocols can then rely on the common mapping and utilize translators to connect heterogeneous components with little integration costs. This paper describes the state of the specification and potential uses of the GEMS specification in Satellite Ground Systems. Interactions between the GEMS specification and other standards such as the CCSDS SLE Complex Management services are also presented.

Mestrado em Engenharia Eletrónica e Telecomunicações
The digital revolution of the 21st century contributed to stem the Internet of Things (IoT). Trillions of embedded devices using the Internet Protocol (IP), also called smart objects, will be an integral part of the Internet. In order to support such an extremely large address space, a new Internet Protocol, called Internet Protocol Version 6 (IPv6) is being adopted. The IPv6 over Low Power Wireless Personal Area Networks (6LoWPAN) has accelerated the integration of WSNs into the Internet. At the same time, the Constrained Application Protocol (CoAP) has made it possible to provide resource constrained devices with RESTful Web services functionalities. This work builds upon previous experience in street lighting networks, for which a proprietary protocol, devised by the Lighting Living Lab, was implemented and used for several years. The proprietary protocol runs on a broad range of lighting control boards. In order to support heterogeneous applications with more demanding communication requirements and to improve the application development process, it was decided to port the Contiki OS to the four channel LED driver (4LD) board from Globaltronic. This thesis describes the work done to adapt the Contiki OS to support the Microchip TM PIC24FJ128GA308 microprocessor and presents an IP based solution to integrate sensors and actuators in smart lighting applications. Besides detailing the system’s architecture and implementation, this thesis presents multiple results showing that the performance of CoAP based resource retrievals in constrained nodes is adequate for supporting networking services in street lighting networks.
A revolução digital do século 21 contribuiu para o surgimento da Internet das Coisas (IoT). Em breve triliões de dispositivos embutidos usando o Internet Protocol (IP) serão parte integrante da Internet. De modo a suportar tal gama de endereços, um novo protocolo de Internet, chamado Internet Protocol versão 6 (IPv6) está a ser adoptado. O IPv6 over Low power Wireless Personal Area Networks (6LoWPAN) acelerou a integração das redes sem-fios de sensores na Internet. Ao mesmo tempo, o Constrained Application Protocol (CoAP) tornou possível fornecer funcionalidades de serviços Web RESTful a dispositivos com recursos limitados. Este trabalho baseia-se em experiências anteriores em redes de iluminação pública, para os quais um protocolo proprietário, elaborado pelo Lighting Living Lab, foi implementado e usado durante vários anos. O protocolo proprietário tem sido utilizado numa ampla gama de placas de controlo de iluminação. De modo a suportar aplicações heterogéneas com requisitos de comunicação mais exigentes além de melhorar o processo de desenvolvimento de aplicações, adaptou-se o Contiki OS à placa LED driver de 4 canais (4LD) da Globaltronic. Esta dissertação descreve o trabalho conduzido para adaptar o Contiki OS ao microprocessador Microchip TM PIC24FJ128GA308 e apresenta uma solução baseada em IP para integrar sensores e atuadores em aplicações de iluminação inteligentes. Além da descrição da arquitetura e da implementação do sistema, este trabalho apresenta vários resultados que mostram que o desempenho do protocolo CoAP na placa 4LD é adequado para suportar serviços Web em redes de iluminação pública.

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Coordenação de Aperfeiçoamento de Pessoal de Nível Superior
A desnutrição grave diminui a imunocompetência e com isso, aumenta a mortalidade infantil. Com o intuito de minimizar a morbimortalidade hospitalar, a OMS publicou diretrizes específicas para o tratamento de crianças com desnutrição grave. Portanto, supõe-se que na alta hospitalar os pacientes apresentem melhora da resposta imunológica. Esta dissertação originou o artigo intitulado Resposta imune de crianças desnutridas graves tratadas segundo o protocolo da OMS na fase hospitalar, atualmente submetido para publicação, e que está apresentado às páginas 25-42. O objetivo foi comparar reposta imune inata de crianças desnutridas graves internadas, tratadas segundo o protocolo da OMS, no momento da admissão e alta hospitalar. Para tal, foi realizado estudo experimental realizado com crianças menores de dois anos de idade, sendo 10 com desnutrição grave, e 10 crianças do grupo controle. O grupo desnutrido foi composto por pacientes internados no Instituto Materno Infantil Professor Fernando Figueira (IMIP) e submetidas ao protocolo da OMS. Foram excluídas as crianças portadoras do vírus HIV e aquelas reinternadas no período do estudo. Foi coletada uma amostra sanguínea na admissão e outra na alta hospitalar, sendo realizada análise do perfil leucocitário, índice de aderência, capacidade fagocitária e a produção de radicais livres (superóxido e óxido nítrico). Os pacientes com desnutrição grave apresentaram na admissão redução significativa da atividade fagocítica e produção de radicais oxidantes. Na alta hospitalar, foi observado melhora da função fagocitária e da liberação de óxido nítrico e superóxido, em relação à admissão hospitalar (p<0,005), entretanto, quando comparados ao grupo controle, os pacientes apresentaram valores reduzidos de linfócitos, assim como diminuição na produção de radicais livres. A partir destes resultados, foi observado que o tempo de hospitalização mostrou-se eficaz em restabelecer a atividade fagocitária, porém insuficiente em restaurar a atividade microbicida e o número de linfócitos

Магістерська робота на тему «Розробка програмної системи для діагностики автомобілів на основі протоколу OBD-II» Топоровського Віталія Васильовича. – Тернопільський національний технічний університет імені Івана Пулюя, Факультет комп'ютерно-інформаційних систем і програмної інженерії, Кафедра програмної інженерії, група СПм–61 // Тернопіль, 2019. C. – 90, рис. – 33, табл. – 6, слайдів. – 12, додат. – 4, бібліогр. – 22. Метою дипломної роботи є дослідження та розробка технології для системи дистанційної діагностики автомобілів. Використовуючи для з’єднання із автомобілеми діагностичний протокол OBD-II та зовнішній пристрій-адаптер ELM327. В даній магістерській роботі розглянуто головні засоби для розробки діагностичного програмного забезпечення, проведено їх аналіз та визначено найкращий засіб для реалізації поставленої задачі. Методи та програмні засоби, використані при виконанні розробки системи: мова програмування C# та її бібліотеки, середовище розробки Visual Studio, база даних MySQL, інтерфейс програмування додатків Windows Forms та супутні програми. Результатом роботи є готовий програмний засіб, який можуть використовувати як звичайні автолюбителі так і працівники автомобільних сервісних центрів. Ключові слова: АВТОМОБІЛЬНА ДІАГНОСТИКА, ЕЛЕКТРОННИЙ БЛОК КЕРУВАННЯ, АНАЛІЗ НЕСПРАВНОСТЕЙ, АВТОМАТИЗАЦІЯ, ПРОГРАМНА СИСТЕМА, ПРОТОКОЛ ПІДКЛЮЧЕННЯ.

The main purpose of this project is to analyse used protocols in vehicles available under the OBD II specifications. In the project we will also focus on processing diagnostic data. We will design device for vehicle diagnostic. This device will be able to send processed data on external web server via Internet network. Obtained data will be saved on data server. Building of functional receiver will be the major part of master´s thesis.

Rumen microbiota enable dairy cattle to breakdown fiber into useable energy for milk production. Rumen bacteria, protozoa, and fungi ferment feedstuff into volatile fatty acids (VFA), the main energy source, while methanogens utilize fermentation by-products to produce methane. Milk fat contains several bioactive rumen-derived fatty acids (FA), including odd-chain FA (OCFA) and branched-chain FA (BCFA), important for maintenance of human health. The overarching dissertation goal was to determine which factors affect rumen methanogen and protozoal community structures and their metabolism products, while defining relationships between rumen microbiota and animal performance. Results presented contribute to the goals of providing new knowledge to dairy farmers, maintaining ruminant health, and enhancing bioactive FA in milk. The first objective was to use next-generation sequencing techniques to determine if lactation stage and dairy breed affect rumen methanogen and protozoal community structures and protozoa cell FA compositions in Jersey, Holstein, and Holstein-Jersey crossbred cows at 3, 93, 183, and 273 days in milk (DIM). A core methanogen community persisted by lactation stage and breed. At 3 DIM, methanogen 16S rRNA gene sequences formed distinct clusters apart from 93, 183, and 273 DIM, reflective of the dietary transition period post-partum. The starch-utilizing protozoal genus Entodinium, was more abundant in Holsteins than in Jerseys and Holstein-Jersey crossbred cows and positively correlated with milk yield. Jerseys had greater iso-BCFA contents in protozoa and milk and protozoa of the genus Metadinium. The second objective was to determine if supplementation of mixed cool-season grasses with annual forages (AF) alters the forage, microbial, and milk FA contents during typical periods of decreased pasture growth in Northeastern US. In short-term grazing (21d) of AF, ruminal VFA and major rumen-derived FA were not altered in bacterial and protozoal cells, suggesting little alteration of biohydrogenation and maintenance of ruminant health. In spring, milk contents of iso-15:0 and 17:0 per serving of whole milk were greater in control (CON)-fed cows, while contents of 12:0 and 14:0 per serving were greater in AF-fed cows. Contents of de novo FA and OCFA per serving of whole milk were greater in summer AF-fed cows than CON-fed cows, while total contents and BCFA did not differ, suggesting post-ruminal FA modifications in adipose tissue and the mammary gland. The third objective was to characterize and relate the rumen microbiota from CON- and AF-fed cows to animal performance. Rumen protozoal taxa were not altered, while less abundant bacterial taxa (< 5%) were different in both periods. The protozoal genus Diplodinium was positively correlated with feed efficiency and milk fat yield. In spring, AF-fed cows had greater abundances of the methanogen species Methanobrevibacter millerae, whereas CON-fed cows had greater abundances of the methanogen species Methanobrevibacter ruminantium, potentially as a result of differences in substrate availability. In conclusion, the work presented identifies several factors that influence rumen microbiota, rumen microbial FA, and milk FA, while providing new information to dairy farmers, researchers, and consumers.

Les buts de cette thèse de doctorat étaient d'explorer les relations entre plusieurs paramètres de performance physique et les paramètres osseux chez un groupe d'homme cinquantenaires, de comparer les indices de résistance osseuse du col fémoral chez un groupe d'homme cinquantenaires inactifs et des anciens joueurs de football de même âge et d'explorer les effets d'un an de football récréatif sur la densité minérale osseuse et les paramètres de performance physique chez un groupe d'hommes cinquantenaires. Trois principales études ont été menées. La première étude a démontré que la VO₂ max (L/min), la masse maigre et la puissance maximale des membres inférieurs sont les meilleurs déterminants des paramètres osseux chez les hommes cinquantenaires. La deuxième étude a démontré que l'ancienne pratique du football est associée à une augmentation des indices de résistance osseuse du col fémoral chez les hommes cinquantenaires. La troisième étude a démontré que le CMO CE, la DMO CF, le CSMI, le CSI, le BSI et l'ISI ont augmenté dans les deux groupes expérimentaux (RF30 et RF60) mais pas dans le groupe témoin. Les pourcentages de variation des paramètres osseux et des performances physiques n'étaient pas significativement différents entre les deux groupes expérimentaux. Le football récréatif est une méthode efficace pour l'amélioration des paramètres de santé osseuse chez les hommes cinquantenaires
The aim of this PhD thesis were to explore the relationships between several physical performance variables and bone parameters in a group of middle-aged men, to compare composite indices of femoral neck strength in inactive middle-aged men and ages-matched former football players and to explore the effects of a 1-year recreational football protocol on bone mineral density and physical performance parameters in a group of healthy inactive 50-year-old men. Three main studies have been conducted. The first study has shown that VO₂ max (L/min), lean mass and maximum power of the lower limbs are the strongest determinants of bone variables in middle-aged men. The second study has shown that former footbal practice is associated with higher composite indices of femoral neck strength in middle-aged men. The third study has demonstrated that WB BMC, FN BMD, CSMI, CSI, BSI and ISI increased in both experimental groups (RF30 and RF60) but not in the control group. The percentages of variations in bone health parameters and in physical performance variables were not significantly different in both experimental groups. Recreational football is an effective method to improve bone health in middle-aged men

La régulation du commerce international des organismes génétiquement modifiés (OGM), plus spécifiquement le Protocole de Carthagène est, de manière générale, bien articulée avec le droit européen des OGM. Celle-ci fait cependant apparaître des conflits vis-à-vis du droit de l’OMC. Une série de divergences s’observe entre ces espaces normatifs par rapport aux principes de précaution et du libre-échange, ainsi qu’aux mécanismes de mise en œuvre relatifs au Protocole de Carthagène. Malgré l’existence de divergences, une série de convergences existe. Ces éléments de rencontre tiennent principalement aux objectifs qui, dans une plus ou moins grande mesure, sont présents dans ces espaces juridiques. Ces objectifs sont principalement la protection de la santé et l’environnement, le développement durable et la protection du libre-échange. Ceci nous amène à nous demander si ces convergences peuvent constituer des « passerelles » dans le cadre d’une démarche d’articulation des espaces juridiques. Cette articulation devient essentielle à l’heure où le droit est de plus en plus fragmenté et qu’un souci de cohérence s’impose. Les clauses de conflit prévues dans le préambule du Protocole de Carthagène ainsi que le principe du soutien mutuel sont les mécanismes qui permettent une mise en cohérence entre ces espaces juridiques. Cependant, cette articulation ne sera jamais véritablement mise en place si aucune ouverture de l’OMC n’est effectuée. Une évolution de l’OMC apparaît ainsi impérative et sous-tend de la part de ses États membres un effort politique. Outre cet effort politique, le juge de l’OMC devra lui aussi faire une interprétation évolutive du droit de l’OMC
Comparative study between the international trade law of genetically modified organisms (GMO) and the European law.The international trade regulation concerning genetically modified organisms (GMO), more specifically the Cartagena protocol, is in general, well-articulated with the European GMO law. This regulation is however in conflict with the WTO law. Between these regulatory systems, divergences are observed involving the precautionary principle and the principle of free trade, as well as with the implementation of mechanisms provided by the Cartagena Protocol. Despite the divergences there are important convergences within the common objectives of health and environmental protection, sustainable development and free trade protection. These convergences let us reflect upon the possibility of creating bridges to allow the articulation between these regulatory systems. This articulation becomes very important in a time where the law is strongly fragmented, and consistency becomes imperative. The conflict clauses established in the preamble of the Cartagena protocol and the mutual supportiveness principle are the mechanisms which allow the possibility of consistency between these regulatory systems, however, an articulation will never be possible before the WTO is open to consider other regulations. For a successful opening of the WTO, its Members States should make a political effort to evolve the WTO; furthermore, the WTO judge should consider an evolutionary interpretation of the WTO law

OBJETIVO: Cuantificar la adherencia del Hospital Santa Rosa Nivel III-1 MINSA de Lima al protocolo de la OMS para el manejo de deshidratación por diarrea aguda en niños de uno a cinco años. MATERIAL Y MÉTODOS: Estudio descriptivo, observacional retrospectivo . Se revisaron historias clínicas de pacientes de 01 a 05 años durante el periodo Enero-Diciembre 2014, con diagnostico de Deshidratación por Diarrea Aguda.. En el análisis e interpretación de la información los resultados se presentaron en tablas de distribución de frecuencias unidimensionales y bidimensionales con sus valores absolutos y porcentuales. Los resultados fueron expresados en cifras absolutas y relativas, el análisis se realizó a través de medidas de tendencia central, χ2 y odds ratio con sus respectivos intervalos de confianza. RESULTADOS: Se revisaron un total de 286 historias clínicas de las cuales el 72% presentó Diagnóstico de Diarrea Aguda sin Deshidratación, 26.6% Deshidratación Leve-moderada y 1.4% Deshidratación Severa. En el 80.1%. de los pacientes tratados hubo Adherencia al Protocolo OMS. En cuanto al tipo de deshidratación se presentó adherencia del 100% en la Deshidratación Severa, 56.6% para Deshidratación Leve-moderada y, 89.3 para los casos de Diarrea Aguda sin Deshidratación. CONCLUSIONES: La adherencia del personal médico al Protocolo de la OMS para el Manejo de Deshidratación por Diarrea Aguda en niños de 01 a 05 años en el Hospital Santa Rosa Nivel III-1 MINSA de Lima es de 80.1%.

The aim of this thesis is to shed light on language variation and language change in judicial protocols from the municipal court in Stockholm during the period 1476−1626. These documents provide a unique insight into late medieval and early modern use of written vernacular. The main material consists of 700 courtroom notes from seven different periods of time, a hundred documents from each year: 1476, 1499, 1525, 1550, 1575, 1600, and 1626. The study draws theoretically on functional linguistics; more specifically, it utilizes Halliday’s systemic-functional grammar, Ulf Teleman’s (1985) theoretical model of language change, as well as dialogism. The results are presented in four analytical chapters. In the first of these, the aim is to systemize the somewhat heterogeneous material, and the corpus is divided in two different ways: one due to judicial content (‘categories of matter’), and one due to textual structure (‘discourse levels’). These systemizations also serve as a methodological foundation for the lexicogrammatical analysis in the following chapters. A main result is that multi-party cases over time develop a functional need for new communication strategies, while unilateral cases already from the beginning seem to bear a more deep-rooted textual stability. In addition, there is a significant increase of discourse level 3, representing communicative events outside the courtroom, in multi-party cases found in texts from 1600 and 1626. In the two following chapters, lexicogrammatical resources of ‘personal reference’ and ‘time and tense’ are analysed. The texts realize different patterns of anaphora, where individuation explains much of the variation: texts with high degree of individuation (criminal cases) materialize a high degree of pronouns, whereas texts regarding property issues materialize low individuation with few pronouns and many full NPs. Regarding the use of tense, the past tense is the most common tense. Still, the study shows an diachronic increase in the use of present tense. The last analytical chapter examines the use of three lexical features: judicial pronouns; word pairs; and nominalizations. The results show that judicial pronouns and word pairs typically occur in registrations matters; nominalizations occur throughout the genre. In the last chapter, the results and implications of the thesis are summated and discussed. The results point towards a pragmatic use of the written language. Although the lexicogrammatical resources are the same during the period, the usages vary. Hence, linguistic variation and change are highly motivated by an intercommunion of contextual factors such as a more solid administrative literacy, a changing litigation, and an increase of legal demands for linguistic precision and documentation. It is argued that the language variety in the genre can be understood as instantiations of different registers.

Consumers of digital contents are empowered with numerous technologies allowing them to produce perfect copies of these contents and distribute them around the world with little or no cost. To prevent illegal copying and distribution, a technology called Digital Rights Management (DRM) is developed. With this technology, consumers are allowed to access digital contents only if they have purchased the corresponding licenses from license issuers. The problem, however, is that those consumers are not allowed to resell their own licenses- a restriction that goes against the first-sale doctrine. Enabling a consumer to buy a digital license directly from another consumer and allowing the two consumers to fairly exchange the license for a payment are still an open issue in DRM research area. This thesis investigates existing security solutions for achieving digital license reselling and analyses their strengths and weaknesses. The thesis then proposes a novel Reselling Deal Signing (RDS) protocol to achieve fairness in a license reselling. The idea of the protocol is to integrate the features of the concurrent signature scheme with functionalities of a License Issuer (LI). The security properties of this protocol is informally analysed and then formally verified using ATL logic and the model checker MOCHA. To assess its performance, a prototype of the RDS protocol has been developed and a comparison with related protocols has been conducted. The thesis also introduces two novel digital tokens a Reselling Permission (RP) token and a Multiple Reselling Permission (MRP) token. The RP and MRP tokens are used to show whether a given license is single and multiple resalable, respectively. Moreover, the thesis proposes two novel methods supporting fair and secure digital license reselling. The first method is the Reselling Deal (RD) method which allows a license to be resold once. This method makes use of the existing distribution infrastructure, RP, License Revocation List (LRL), and three protocols: RDS protocol RD Activation (RDA) protocol, and RD Completion (RDC) protocol. The second method is a Multiple License Reselling (MLR) method enabling one license to be resold N times by N consumers. The thesis presents two variants of the MLR method: RRP-MR (Repeated RP-based Multi-Reselling) and HC-MR (Hash Chain-based Multi-Reselling). The RRP-MR method is designed such that a buyer can choose to either continue or stop a multi-reselling of a license. Like the RD method, the RRP-MR method makes use of RP, LI, LRL, and the RDS, RDA, and RDC protocols to achieve fair and secure reselling. The HC-MR method allows multiple resellings while keeping the overhead on LI at a minimum level and enable a buyer to check how many times a license can be further resold. To do so, the HC-MR utilises MRP and the hash chain cryptographic primitive along with LRL, LI and the RDS, RDA and RDC protocols. The analysis and the evaluation of these three methods have been conducted. While supporting the license reselling, the two methods are designed to prevent a reseller from (1) continuing using a resold license, (2) reselling a non-resalable license, and (3) reselling one license a unauthorised number of times. In addition, they enable content owners of resold contents to trace a buyer who has violated any of the usage rights of a license bought from a reseller. Moreover, the methods enable a buyer to verify whether a license he is about to buy is legitimate for re-sale. Furthermore, the two methods support market power where a reseller can maximise his profit and a buyer can minimise his cost in a reselling process. In comparison with related works, our solution does not make use of any trusted hardware device, thus it is more cost-effective, while satisfying the interests of both resellers and buyers, and protecting the content owner's rights.

Proteins play a central role in all the biological processes. The immense diversity in protein structures and functions despite similar underlying composition is intriguing. The work presented in this thesis aims to provide a deeper understanding of protein structure-function relationships. It describes some techniques that were developed in order to probe these relationships. Chapter 1 provides a general introduction to the topics discussed in the thesis. Chapter 2 focuses on an important aspect of protein structures, the cavities. Although proteins are composed of regular arrangements of secondary structures, namely, α helices and β sheets, there are some irregularities. The packing density is not uniform throughout the protein resulting into formation of cavities. The role of cavities has been previously probed using mutagenesis studies. The mutations designed to fill the cavities were observed to improve stability and cavity creating mutations adversely affected the stability. Cavities are thus reported to be important contributors to stability. In chapter 2, we refine and benchmark a method for prediction of protein cavities based on molecular dynamics simulations. The insights derived from the mutagenesis studies provide some basic understanding of substitution preferences in proteins. The exposed non-active site positions are more tolerant to mutations whereas, the buried positions are not. Introducing cavity filling and disulfide mutations in proteins have been demonstrated to improve stability. Engineering protein variants with improved stability has immense applications in biology. In chapter 3, we discuss an important application, i.e., immunogen designing. Surface glycoproteins of several viruses exhibit two conformations, namely the metastable prefusion conformation and the highly stable postfusion conformation adopted during the fusion of the virus with the host cell membrane. Stable immunogens exhibiting the prefusion conformation are promising candidates for subunit vaccines. In chapter 3, we discuss immunogen designing for the respiratory syncytial virus (RSV). In addition to stabilized mutants, temperature sensitive (Ts) mutants are another class of engineered proteins. The Ts mutants exhibit reduced activity levels above the permissive temperature. They have been extensively used in developmental biology. Ts mutants are excellent tools to modulate protein expression levels in cells. A model for prediction of Ts positions was developed previously. This model exploits residue hydrophobicity to infer residue burial in the structure solely based on the protein sequence. In the current work, we improved the accuracy of the model by incorporating structural information in the model. Chapter 4 describes the development and benchmarking of a server for the prediction of Ts mutants (TSpred). The TSpred server suggests a stereochemically diverse set of mutations at the putative buried positions which are would produce destabilization to different extents and at least one of them is likely to be Ts. The TSpred predictions were employed for designing live attenuated vaccine candidates for RSV. The work thus far elaborates on factors contributing to protein stability and application of that information for rationally designing mutants to modulate protein structure and function. In order to gain a deeper understanding of the role of each protein residue in its function, simultaneous analysis of multiple mutants is essential. Site saturation mutagenesis techniques generate all nineteen mutations at each residue position of the protein and the mutant function is linked to a phenotypic readout like cell viability or binding to a ligand. The mutant libraries are deep sequenced using one of the available platforms like Illumina, SOLiD, 454 and Ion torrent, and analysed to estimate the relative proportions of each of the mutants in the library. Automated programs are necessary to analyse the large amount of data generated after deep sequencing. A pipeline for analysis of data generated from the Illumina sequencing platform is discussed in chapter 5. A mutational sensitivity measure denoted as MSseq was previously derived for the Controller of Cell division or Death B protein (CcdB). The values of the MSseq parameter reflect mutant activity. The active or inactive phenotypes of various mutants were analysed as a function of residue burial. Additional insights about substitution preferences at buried positions were gained from this analysis. In addition to residue burial, the substitution preferences varied with the physico-chemical nature and the size of the wild type (WT) and the mutant side chains. The active site of the CcdB protein could be inferred based on the trends in the mutational sensitivity values. We quantitated these effects and developed a model, detailed in chapter 6, for prediction of the mutant phenotypes using a fraction of the CcdB mutational data. The model was observed to perform better than two other machine learning based predictors, SNAP2 and SuSPect. Chapter 7 describes an additional application of the mutational sensitivity data. By analysing the population distribution of the MSseq values, an empirical parameter, RankScore, was previously derived for each residue in CcdB. RankScore can be interpreted as a weighted average of the MSseq values. RankScore was found to correlate well with residue depth which measures the extent of burial of a residue. As the residue depths in the native structure correlated well (r = 0.6) with the RankScores, the residue depths in native-like models would also correlate well with RankScores. Based on this principle, native-like models could be distinguished from low quality decoys. In the analysis reported in chapter 7, we examine this methodology using decoy datasets for ~200 proteins. We also consider additional information like predicted secondary structure and model quality score to achieve better model discrimination. Studies thus far describe analyses performed using single protein mutants. Furthermore, information about residue interactions is also important. During the course of evolution, as the maintenance of specific interactions is essential for protein function, residues participating in such interactions are either conserved or varied in a correlated manner. Several computational models analysing such correlations among mutations are available. Experimental techniques are also available for identification of the spatially proximal residues. In chapter 8, we analyse various computational programs using CcdB and Diacylglycerol kinase A (DgkA) proteins and the results are then compared with the available experimental data. Overall little overlap was observed between the predictions based on the natural sequence co-variation and the experimental data. Both the computational and experimental approaches can be applied in conjugation as they provide complementary information. The analyses described in the current work would provide useful guidelines for rational design of mutations to modulate protein stability. This has important implications in immunogen designing. The tools developed as part of the current work can be applied for (i) rational designing of Ts mutants, (ii) the analysis of site saturation libraries, (iii) calculation or prediction of substitution preferences, (iv) structure prediction using correlated mutations as constraints, or (v) protein model discrimination. A small appendix section is also included in the thesis. Synonymous mutations with differential phenotypes were observed in our deep sequenced library. In order to analyse them further, we performed a multiple sequence alignment and analysed codon frequencies at different positions. However, only preliminary results are available and those are included in Appendix I section of this thesis.

O uso de modelos baseados em agentes (MBA) é uma abordagem popular para o desenvolvimento de simulações em domínios como as ciências sociais, a geografia e as ciências naturais. Entretanto devido a complexidade dos modelos, a partilha das especificações torna-se um processo penoso, que dificulta a validação e replicação deste tipo de modelo. Para auxiliar nesse processo, foi desenvolvido o protocolo ODD que torna mais fácil a escrita e leitura das descrições dos MBA. Este protocolo evoluiu e foi refinado nas versões ODD+D e ODD+2D. Contudo, por se tratar de um protocolo, a latitude permitida na descrição dos modelos e o formato em que são disponibilizadas estas descrições dificultam, por um lado a compreensão da descrição de maneira geral, e por outro lado o próprio acesso e utilização destes modelos. As especificações dos MBA podem ser encontradas em repositórios de modelos e artigos científicos acessíveis apenas por pesquisas manuais. Para solucionar o problema foi desenvolvida uma framework , baseada numa ontologia para permitir a rastreabilidade dos modelos. Foram incluídos conceitos de outras ontologias para descrever os autores e o código informático associado a cada MBA. Foi desenvolvida uma aplicação que facilita o acesso aos modelos e que permite a análise da proveniência dos dados dos modelos e a extração das componentes do protocolo. Foi também desenvolvida uma API que permite aceder e processar as descrições de cada MBA. A aplicação foi submetida a uma série de testes que validam a abordagem usada e a utilidade da framework.
The use of agent-based models (ABM) is a popular approach to develop simulations in fields such as social sciences, geography and natural sciences.. However, due to the complexity of the models, the sharing of specifications becomes a painful process, which makes it difficult to validate and replicate this type of model. To assist in this process, the ODD protocol was developed, which makes it easier to write and read the ABM descriptions. This protocol evolved and was refined in the ODD + D and ODD + 2D versions. However, because it is a protocol, the latitude allowed in the description of the models and the format in which these descriptions are available make it difficult, on the one hand, to understand the description in general, and on the other hand, to access and use of these models. ABM specifications can be found in repositories of models and scientific articles accessible only by manual searches. To solve the problem, a framework was developed, based on an ontology to allow traceability of the models. Concepts from other ontologies were included to describe authors and document data and the computer code associated with each ABM. An application was developed to facilitate the access to the models, which allows the analysis of the source of the model data and the extraction of the protocol components. An API was also developed that allows access and process the descriptions of each MBA. The application was subjected to a series of tests that validate our approach and the utility of the framework.

The Internet of Things (IoT) might be the next revolutionary technology to mark a generation. It could have a particularly strong influence on the automotive industry, changing people’s perception of what a vehicle can do. By connecting several things in a car, IoT empowers it to sense and communicate. Furthermore, this technology clearly opens the way to emerging applications such as automated driving, Vehicle-to-Vehicle and Vehicleto-Infrastructure communication. Vehicle’s information about its environment and surroundings is crucial to the development of existing and emerging applications. It is already possible to communicate directly (on-site) with vehicles through a built-in On Board Diagnostics (OBD), making it possible to obtain crucial information about the state of the vehicle in real environments. However, there is zero tolerance for error when developing new applications for vehicles that are, a priori, extremely costly and that must also safeguard human lives. Therefore, there is an increasing need for OBD emulators which can allow the development of new applications. This Thesis proposes a model-driven architecture for multi-protocol OBD emulator, encouraging the development of new emerging OBD systems in a safety environment, to promote the creation of applications to interact or use vehicles’ data. In this sense, the addressed specifications are: Less expensive comparing with today’s solutions; Compatible with different OBD protocols communication; Open Source Hardware and Software suitable for Do-It-Yourself (DIY) development.

碩士
國立交通大學
電機資訊國際學程
103
IoT/M2M aims to integrate a large number of heterogeneous devices which are constantly required to be discovered and reconfigured in order to bring them up to date or to recover their failures. The device management protocol is needed to reduce the amount of time to configure and to manage those IoT/M2M devices, especially for the M2M devices located in the remote area. The device management protocol enables abstraction of an IoT/M2M device as a managed object to ease the management of device; to do this for IoT/M2M devices, this abstraction has to be concise and lightweight. The IoT/M2M international standard organizations, such as ETSI and OneM2M, proposed the use of lightweight device management protocol called Open Mobile Alliance Lightweight M2M (OMA LWM2M) protocol to manage and to configure the IoT/M2M devices from IoT/M2M platform. By incorporating device management in the IoT/M2M platform, we can establish device management sessions that allow an IoT/M2M application to manage IoT/M2M devices remotely from the IoT/M2M service platform. For example, by manipulating resource trees in the cyber space from the IoT/M2M platform we gain access and control to the corresponding sensors/devices in the physical world. However, how to perform resource mapping of OMA LWM2M in the IoT/M2M standard architecture platform is not well addressed by the standard. In this research, we examine how to incorporate the OMA LWM2M in the M2M standard architecture and we analyze how the M2M standard architecture can collaborate and communicate with OMA LWM2M protocol to establish device management sessions. For the experimental process, we analyze the resource mapping of OMA LWM2M objects and resources into the ETSI M2M resource tree by analyzing the processes of (1) Bootstrapping, (2) Registration, and (3) Management and Service Enablement Session. 1. The bootstrapping process is used to provision the OMA LWM2M objects to the IoT/M2M platform for device management session. 2. The registration process is used to register the OMA LWM2M Client and its Objects in the OMA LWM2M Server. This process is analyzed during the device triggering session. 3. The Device Management and Service Enablement Session is a procedure of accessing Object or Resource of the OMA LWM2M Client by an M2M application using OMA LWM2M Server. In order to proceed with our implementation and analysis, we develop Remote Entity Management (REM) Service Capability on top of the OpenMTC, an ETSI M2M standard compliant system from Fraunhofer FOKUS. We also utilize Eclipse Leshan and Wakaama that are the OMA LWM2M open-source reference implementations to implement the REM Capability. To proceed with the analysis, we build an IoT/M2M application and do features testing on it. The feature testing includes the manipulation of the device objects and resources using RESTful commands, the simulation of firmware update mechanism, and the simulation of the remote reboot mechanism for fault management.

碩士
國立彰化師範大學
電子工程學系
104
Accompanying with innovation of vehicle technique, the performance of vehicles has been massively enhanced. The scale of electronic control units has been increased and became more complicate. Therefore, the vehicle safety guard mechanism has become an important research topic of advanced vehicle development. Currently, the development of vehicle safety guard mechanisms was supported by OBD protocol to obtain the dynamic behavior information of vehicles. But it is difficult to reduce the cost of actual vehicle road test, the manpower, and risk. They became challenges of vehicle safety system development. The Hardware-in-the-Loop simulator is able to generated dynamic behavior mostly closed to the target physical system. Also, it has been proved to overcome the difficulties of road test cost, manpower, and risk. In this paper, an OBD protocol simulator based on a HIL engine has been developed to build up a closed real vehicle dynamic model to support the function verification and test of safety guard system development. Furthermore, it specifically simplifies the application porting to on real vehicles.

This diploma thesis, processed under the name: External system of measuring parameters of vehicles with support of diagnostic protocols integrated in Ethernet networkon a cylindrical dynamometer, deals with the definition of parameter variables that are important for vehicle tests on a cylindrical dynamometer. After determining these variables, a measuring module, which is designed to retrieve data from individual sensors located in the vehicle, is implemented via an OBD II diagnostic connector connected to the vehicle data bus CAN-BUS. The measuring module is integrated into the existing cylindrical dynamometer system at the Mendel University Testing Center in Brno, where the system was tested on a real vehicle.

Měšťanské domy Starého Města pražského ve světle vizitačního protokolu tereziánského katastru. Abstrakt Vývoj staroměstské domovní zástavby během barokní epochy bývá nejčastěji posuzován z uměleckohistorického hlediska. V této práci je na domy Starého Města pražského pohlíženo optikou berních pramenů hromadné povahy. Výchozí časový mezník představuje rok 1653, kdy se v pražských městech započalo se sestavováním prvního českého katastru - berní ruly. Komparací údajů berní ruly a vizitačního protokolu tereziánského katastru z let 1725-1726 jsem se pokusila odpovědět na otázku, jaké byly vývojové trendy v domovním majetku staroměstských měšťanů. Písemný výsledek vizitace tereziánského katastru představuje mimořádný pramen, který eviduje řadu podstatných údajů pro dějiny staroměstských domů. Srovnání s berní rulou je v tomto případě omezené výpovědní hodnotou staršího katastru, který nepřináší tolik podrobných informací jako vizitační protokol. Analýzu bylo možné provést jen v těch indikátorech, které se týkají počtu, skladby a daňového zatížení domů. Výrazné změny v počtu staroměstských domů sice berní prameny v letech 1653-1726 neprokazují, zaznamenávají ale odlišnou situaci v samotné skladbě domů. Za hlavní tendence, které se projevily ve vývoji domovní zástavby Starého Města pražského, lze označit zvýšení...