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Artykuły w czasopismach na temat "Newport High School (Newport, Kentucky)"
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Egorova, Svetlana A., Lidiya A. Kaftyreva, Lyudmila V. Suzhaeva, Anna V. Zabrovskaia, Elena V. Voitenkova, Zoya N. Matveeva, Yulia V. Ostankova i in. "ANTIMICROBIAL RESISTANCE AND CLINICAL SIGNIFICANT RESISTANCE MECHANISMS OF SALMONELLA ISOLATED IN 2014-2018 IN ST.PETERSBURG, RUSSIA". Russian Clinical Laboratory Diagnostics 64, nr 10 (15.10.2019): 620–26. http://dx.doi.org/10.18821/0869-2084-2019-64-10-620-626.
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The article presents the results of antimicrobial resistance monitoring of Salmonella isolated from children and adults with diarrhea in St. Petersburg in 2014-2018. In 746 isolates of 42 serovars more than 90,0% belonged to three: S. enteritidis (79,6%), S. typhimurium (6,8%) and S. infantis (3,8%). The antimicrobial susceptibility testing (according the EUCAST) to 7 classes of antimicrobials revealed the resistance in 78,6% of Salmonella. Low-level quinolone resistance (MIC of ciprofloxacin 0,12-0,5 mg/l) was detected in 63,3% isolates (S. enteritidis -71,0%, S. typhimurium - 15,7%, S. infantis - 89,3%) and was due to five kinds of single nucleotide substitutions in gyrA: Asp87Tyr - 36,1% of studied isolates (only S. infantis); Ser83Phe - 22,2% (only S. enteritidis); Asp87Asn - 19,4% (S. enteritidis, S. typhimurium, S. hadar, S. newport); Ser83Tyr -11,1% (S. enteritidis and S. infantis) and Asp87Gly - 8,3% (only S. enteritidis). Only in one S. kentucky isolate with high-level fluoroquinolone resistance (MIC of ciprofloxacin > 8,0 mg/l) two substitutions (Ser83Phe and Asp87Asn) were detected. Two Salmonella isolates (S. typhimurium and S. corvallis) had plasmid-mediated quinolone resistance (qnrS). Extended-spectrum cephalosporin resistance was found in 6 Salmonella serovars (1,6%). The bla-genes were detected: of genetic group CTX-M1 - in 10 isolates (serovars S. typhimurium, S. enteritidis, S. abony, S. coeln and S. virchow), CTX-M2 - in 2 S. typhimurium isolates, CTX-M9 - in three S. enteritidis isolates. In one S. typhimurium CTX-M1 and CTX-M2 were detected. The gene of CMY-2 (molecular class C cephalosporinase) was revealed in two isolates (S. newport and S. enteritidis). Our study showed that Salmonella (the main bacterial pathogen of acute diarrhea in children and adults) isolated in Saint-Petersburg had antimicrobial resistance to drugs of choice for salmonellosis treatment.
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Fricke, W. Florian, Patrick F. McDermott, Mark K. Mammel, Shaohua Zhao, Timothy J. Johnson, David A. Rasko, Paula J. Fedorka-Cray i in. "Antimicrobial Resistance-Conferring Plasmids with Similarity to Virulence Plasmids from Avian Pathogenic Escherichia coli Strains in Salmonella enterica Serovar Kentucky Isolates from Poultry". Applied and Environmental Microbiology 75, nr 18 (31.07.2009): 5963–71. http://dx.doi.org/10.1128/aem.00786-09.
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ABSTRACT Salmonella enterica, a leading cause of food-borne gastroenteritis worldwide, may be found in any raw food of animal, vegetable, or fruit origin. Salmonella serovars differ in distribution, virulence, and host specificity. S almonella enterica serovar Kentucky, though often found in the food supply, is less commonly isolated from ill humans. The multidrug-resistant isolate S. Kentucky CVM29188, isolated from a chicken breast sample in 2003, contains three plasmids (146,811 bp, 101,461 bp, and 46,121 bp), two of which carry resistance determinants (pCVM29188_146 [strAB and tetRA] and pCVM29188_101 [bla CMY-2 and sugE]). Both resistance plasmids were transferable by conjugation, alone or in combination, to S. Kentucky, S almonella enterica serovar Newport, and E scherichia coli recipients. pCVM29188_146 shares a highly conserved plasmid backbone of 106 kb (>90% nucleotide identity) with two virulence plasmids from avian pathogenic E scherichia c oli strains (pAPEC-O1-ColBM and pAPEC-O2-ColV). Shared avian pathogenic E. coli (APEC) virulence factors include iutA iucABCD, sitABCD, etsABC, iss, and iroBCDEN. PCR analyses of recent (1997 to 2005) S. Kentucky isolates from food animal, retail meat, and human sources revealed that 172 (60%) contained similar APEC-like plasmid backbones. Notably, though rare in human- and cattle-derived isolates, this plasmid backbone was found at a high frequency (50 to 100%) among S. Kentucky isolates from chickens within the same time span. Ninety-four percent of the APEC-positive isolates showed resistance to tetracycline and streptomycin. Together, our findings of a resistance-conferring APEC virulence plasmid in a poultry-derived S. Kentucky isolate and of similar resistance/virulence plasmids in most recent S. Kentucky isolates from chickens and, to lesser degree, from humans and cattle highlight the need for additional research in order to examine the prevalence and spread of combined virulence and resistance plasmids in bacteria in agricultural, environmental, and clinical settings.
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Yoshida, Catherine, Simone Gurnik, Aaminah Ahmad, Travis Blimkie, Stephanie A. Murphy, Andrew M. Kropinski i John H. E. Nash. "Evaluation of Molecular Methods for Identification of Salmonella Serovars". Journal of Clinical Microbiology 54, nr 8 (18.05.2016): 1992–98. http://dx.doi.org/10.1128/jcm.00262-16.
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Classification by serotyping is the essential first step in the characterization ofSalmonellaisolates and is important for surveillance, source tracking, and outbreak detection. To improve detection and reduce the burden of salmonellosis, several rapid and high-throughput molecularSalmonellaserotyping methods have been developed.The aim of this study was to compare three commercial kits, Salm SeroGen (Salm Sero-Genotyping AS-1 kit), Check&Trace (Check-Points), and xMAP (xMAPSalmonellaserotyping assay), to theSalmonellagenoserotyping array (SGSA) developed by our laboratory. They were assessed using a panel of 321 isolates that represent commonly reported serovars from human and nonhuman sources globally. The four methods correctly identified 73.8% to 94.7% of the isolates tested. The methods correctly identified 85% and 98% of the clinically importantSalmonellaserovars Enteritidis and Typhimurium, respectively. The methods correctly identified 75% to 100% of the nontyphoidal, broad host rangeSalmonellaserovars, including Heidelberg, Hadar, Infantis, Kentucky, Montevideo, Newport, and Virchow. The sensitivity and specificity ofSalmonellaserovars Typhimurium and Enteritidis ranged from 85% to 100% and 99% to 100%, respectively.It is anticipated that whole-genome sequencing will replace serotyping in public health laboratories in the future. However, at present, it is approximately three times more expensive than molecular methods. Until consistent standards and methodologies are deployed for whole-genome sequencing, data analysis and interlaboratory comparability remain a challenge. The use of molecular serotyping will provide a valuable high-throughput alternative to traditional serotyping. This comprehensive analysis provides a detailed comparison of commercial kits available for the molecular serotyping ofSalmonella.
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RADY, M., N. A. EZZ-EL-DIN, K. F. MOHAMED, S. NASEF, A. SAMIR i W. K. ELFEIL. "Correlation between ESβL Salmonella Serovars Isolated from Broilers and their Virulence Genes". Journal of the Hellenic Veterinary Medical Society 71, nr 2 (1.07.2020): 2163. http://dx.doi.org/10.12681/jhvms.23645.
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Salmonella is considered to be one of the major poultry bacterial pathogens. The poultry species are one of the main reservoirs for the human types, thus serving as public health hazards. The development of drug resistant genes and multidrug resistant types from Salmonella has increased recently. This current study was undertaken to estimate the correlation between extended spectrum beta-lactamase multidrug resistant (ESβL) Salmonella serovars isolated from broilers and their virulence genes. Two hundred and forty samples were collected from clinically diseased broilers chicks (showed disorders of the intestinal tract) and examined for the presence of Salmonella isolates according to ISO 6579: 2002 and ISO. 6579-3:2014 Fifty Salmonella isolates were isolated with an incidence of 20.8%. Isolates of Salmonella were serotyped as follows: 25 S. Kentucky, 9 S. Infantis, 6 S. Enteritidis, 4 S. Heidelberg, and one isolates per serovars S. Labadie, S. Typhi, S. Agona, S. Pullorum, S. Newport and S. Virginia. AST (antimicrobial susceptibility testing) showed that high percentage of isolates were resistant to all Ampicillin (90%), Nalidixic acid (88%), Sulfamethoxazole + Trimethoprim (82%) and Tetracycline (82%). Approximately 86% of the isolates demonstrated multiple resistance, of which 18.75% and 25% were resistant to three and four antimicrobial types, respectively. Phenotypic detection of ESβLs by using screening test (Cefinase®) and confirmatory test by using combined disk diffusion test revealed that 32% of isolates were positive for both tests with 20% similarity and 12% diversity between the two tests. Molecular characterization of some ESβLs genes (blaTEM, blaCTX, blaOXA, blaCMY and blaSHV) and some virulence genes (invA, avrA, sopB, bcfC, stn (was done using PCR. The results showed that all the ESβLs positive serovars were positive for amplification of all tested virulence genes and noticed that all the isolates were negativefor blaCMY gene. The present study suggests that virulent ESβL Salmonella serovars could infect broilers and should be taken into consideration as an important bacterial pathogen affecting poultry.
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Glines, Masami N., Keith P. Poulsen i Nicole A. Aulik. "Geospatial and temporal changes in Wisconsin bovine Salmonella enterica isolates between 2006 and 2014". Bovine Practitioner, 1.06.2018, 160–72. http://dx.doi.org/10.21423/bovine-vol52no2p160-172.
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Bovine salmonellosis carries a high economic burden for cattle operations and presents a public health risk due to zoonosis. The objective of this study was to determine the frequency and concurrent location of Salmonella enterica subspecies enterica serovars isolated in Wisconsin from bovine diagnostic samples submitted to the Wisconsin Veterinary Diagnostic Laboratory from 2006 to 2014, by using geographic information system and statistical analysis tools. Salmonella serovars and zip codes of animal location were retrospectively collected. Accessions were limited to bovine fecal and tissue samples that yielded a typeable Salmonella serovar either by culture or polymerase chain reaction. A total of 4,829 accessions were included in the study that yielded definitive Salmonella serovars. The 5 most frequently isolated serovars were Dublin [23%), Cerro (15%), Newport (14%), Kentucky (9%), and Montevideo (9%). After normalization, the region with the highest incidence was the east-central region for all serovars combined. Overall incidence decreased during the 9 years except for Salmonella ser. Cerro, which increased. Incidence of Salmonella serovars in densely populated dairy regions in Wisconsin was variable. Continued study and monitoring of the spatial disease ecology for salmonellosis in Wisconsin is warranted to improve animal health and milk production, and minimize the risk of zoonosis.
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Zhao, Liyuan, Gang Liu, Wenli Tang, Xiangbin Song, Xiaoyu Zhao, Chu Wang, Youzhi Li i Ming Zou. "Antimicrobial resistance and genomic characteristics of Salmonella from broilers in Shandong Province". Frontiers in Veterinary Science 10 (23.11.2023). http://dx.doi.org/10.3389/fvets.2023.1292401.
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IntroductionThe emergence of multidrug-resistant (MDR) strains of Salmonella, which is a genus of important zoonotic pathogens, has aroused great public health concern worldwide.MethodsIn this study, 167 strains of Salmonella were isolated from 947 samples from broiler farms, slaughterhouses, and markets in Shandong Province. Antibiotic sensitivity testing was performed, and 70 strains of Salmonella were screened out by whole-genome sequencing (WGS) to evaluate serotypes, antimicrobial resistance genes (ARGs), the prevalence of class 1 integrons, the plasmid carriage rate, and phylogenetic characteristics and for multilocus sequence typing (MLST).ResultsThe results showed that the 167 isolates showed the highest resistance to ampicillin (AMP, 87.4%), sulfamethoxazole (SF, 87.4%), compound sulfamethoxazole (SXT, 81.4%), nalidixic acid (NAL, 80.2%), and amoxicillin/clavulanic acid (A/C, 77.8%). All the strains were sensitive to meropenem (MEM), and 91.0% of the isolates were MDR strains. We screened a total of 45 ARGs, with the highest detection rate observed for the tetracycline (TET) resistance gene tet (A) (81.4%). A total of 21 types of plasmid replicons were detected in Salmonella, of which IncX1 was the most common (74.3%), and 62.9% of the isolates carried a class 1 integron. In addition, a total of 11 different serotypes were detected, with S. enteritidis as the predominant serovar., followed by S. infantis and S. Newport. Twelve different sequence types (STs) were detected, among which ST11 was the main type. There was a strong correspondence between serotypes and STs. We also found that S. Indiana and S. Kentucky had extremely high rates of resistance to ciprofloxacin (CIP) and third-generation cephalosporins. System-wide genome analysis showed the occurrence of long-distance transmission across fields.ConclusionIn conclusion, the detection of multidrug resistance and isolates carrying multidrug resistance genes is the main problem, and emergency strategies should be implemented to address this issue.
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Harding Bradley, Luke Edward, i Luke Reaper. "An assessment of the impact of COVID-19 related social distancing measures on the stress levels of students". Journal of Student Research 9, nr 2 (20.11.2020). http://dx.doi.org/10.47611/jsrhs.v9i2.1226.
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Since the novel coronavirus was first reported in China in early 2020, governments have aimed to cull its spread and raise awareness of the symptoms of the infection. These measures are collectively known as social distancing and self-isolation.
Prior to COVID-19, 85% of students in university reported feeling overwhelmed by everything they had to do at some point within the prior year (AADA, 2015). Organizations treating teen anxiety and depression are highlighting the impact of isolation on teenagers and young adults and claim an association with a decline in self-care and a greater risk of suicide (Newport Academy, 2020). Given the pre-existing high levels of stress and mental strain experienced by students, the consequences of social isolation may more significantly impact students in comparison with non-students.
This study uses the State-Trait Anxiety Inventory (STAI) index to understand the impact that social distancing and self-isolation has on the mental health of students and non-students globally. 502 responses were gathered globally with most respondents residing in the United States of America, Italy and Ireland.
The results of this study suggest that the COVID-19 related Social Distancing and Self-isolation lead to a greater increase in stress among students than among adults as measured by STAI. The reported stress was highest among high school age students and among female students generally. The reported causes of this stress are primary related to the impact on education of the measures rather than directly related to the health consequences pandemic.
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Carroll, Laura M., Ariel J. Buehler, Ahmed Gaballa, Julie D. Siler, Kevin J. Cummings, Rachel A. Cheng i Martin Wiedmann. "Monitoring the Microevolution of Salmonella enterica in Healthy Dairy Cattle Populations at the Individual Farm Level Using Whole-Genome Sequencing". Frontiers in Microbiology 12 (18.10.2021). http://dx.doi.org/10.3389/fmicb.2021.763669.
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Livestock represent a possible reservoir for facilitating the transmission of the zoonotic foodborne pathogen Salmonella enterica to humans; there is also concern that strains can acquire resistance to antimicrobials in the farm environment. Here, whole-genome sequencing (WGS) was used to characterize Salmonella strains (n = 128) isolated from healthy dairy cattle and their associated environments on 13 New York State farms to assess the diversity and microevolution of this important pathogen at the level of the individual herd. Additionally, the accuracy and concordance of multiple in silico tools are assessed, including: (i) two in silico serotyping tools, (ii) combinations of five antimicrobial resistance (AMR) determinant detection tools and one to five AMR determinant databases, and (iii) one antimicrobial minimum inhibitory concentration (MIC) prediction tool. For the isolates sequenced here, in silico serotyping methods outperformed traditional serotyping and resolved all un-typable and/or ambiguous serotype assignments. Serotypes assigned in silico showed greater congruency with the Salmonella whole-genome phylogeny than traditional serotype assignments, and in silico methods showed high concordance (99% agreement). In silico AMR determinant detection methods additionally showed a high degree of concordance, regardless of the pipeline or database used (≥98% agreement among susceptible/resistant assignments for all pipeline/database combinations). For AMR detection methods that relied exclusively on nucleotide BLAST, accuracy could be maximized by using a range of minimum nucleotide identity and coverage thresholds, with thresholds of 75% nucleotide identity and 50–60% coverage adequate for most pipeline/database combinations. In silico characterization of the microevolution and AMR dynamics of each of six serotype groups (S. Anatum, Cerro, Kentucky, Meleagridis, Newport, Typhimurium/Typhimurium variant Copenhagen) revealed that some lineages were strongly associated with individual farms, while others were distributed across multiple farms. Numerous AMR determinant acquisition and loss events were identified, including the recent acquisition of cephalosporin resistance-conferring blaCMY- and blaCTX–M-type beta-lactamases. The results presented here provide high-resolution insight into the temporal dynamics of AMR Salmonella at the scale of the individual farm and highlight both the strengths and limitations of WGS in tracking zoonotic pathogens and their associated AMR determinants at the livestock-human interface.
Rozprawy doktorskie na temat "Newport High School (Newport, Kentucky)"
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Smith, Wayne Keith. "An Evaluation of the Attendance Policy and Program and Its Perceived Effects on High School Attendance in Newport News Public Schools". Diss., Virginia Tech, 1998. http://hdl.handle.net/10919/30501.
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The purpose of this quantitative study is to determine the effects of the attendance policy and attendance program after one year of implementation in Newport News Public Schools with a total high school population of approximately 5,820 students. The school district recently implemented a new attendance policy and program to address high school student absenteeism.
This multi-faceted study examined the effects of this new policy by conducting statistical analyses of attendance data, programmatic data, and surveys of students, parents, and teachers. This allowed for the examination of the primary question. Whether there is a differential impact of the policy among the grades (9,10,11) during the 1996-1997 school year with respect to high school attendance after adjusting for initial differences on the 1995-1996 high school attendance through the use of an analysis of covariance? Additionally, the perceptions of students, parents, and teachers regarding the new attendance policy were examined along with the degree to which a specially-designed program, Saturday Redemptive School, affected the academic pass rate of participants. Findings show significant main effects and non-significant interaction at the pre-established alpha level of .05. Survey results may have implied a change in the attendance policy and Saturday Redemptive School. The findings from the Chi-square Test were not significant at the pre-established alpha level of .05.
The results of this study provides valuable information in the formulation of attendance policies by local and state inner-city district level administrators regarding the effectiveness of school attendance policy and attendance programs.Ed. D.
Książki na temat "Newport High School (Newport, Kentucky)"
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Bowser, Walter A. To Huntington we pledge: The year not forgotten (1966-1967). Williamsburg, Va: Pub. Connections, 2003.
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Huntington High School, symbol of community hope and unity, 1920-1971: A pictorial history of Huntington High School Newport News, Virginia. Yorktown, VA: Pub. Connections, 1999.
Znajdź pełny tekst źródłaCzęści książek na temat "Newport High School (Newport, Kentucky)"
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Perkins, Linda M. "Fanny Jackson Coppin and Oberlin College". W The Oxford Handbook of American and British Women Philosophers in the Nineteenth Century. Oxford University Press, 2023. http://dx.doi.org/10.1093/oxfordhb/9780197558898.013.22.
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Abstract Fanny Jackson Coppin was a prominent educator of the late nineteenth and early twentieth centuries. Born a slave in Washington, DC, in 1837, her freedom was purchased by a devoted aunt when she was thirteen. She moved to Newport, Rhode Island, where she worked as a servant in the mansion of the Calverts, descendants of Lord Baltimore and Mary Queen of Scots. From 1860 to 1865, Coppin attended Oberlin College in Ohio. When she graduated in 1865, she was the second known Black woman college graduate in the country. Upon graduating, she became a teacher and later principal of the Quaker-founded private classical high school in Philadelphia, the Institute for Colored Youth, working there for thirty-seven years. Under Coppin’s leadership, the school became renowned for its high academic offerings and outstanding students. This chapter discusses her philosophy of education and race.
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Tidwell, John Edgar, i Mark A. Sanders. "“The Palmer Case”". W Sterling A. Brown’s, A Negro Looks At The South, 225–32. Oxford University PressNew York, NY, 2007. http://dx.doi.org/10.1093/oso/9780195313994.003.0037.
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Abstract Before I got to know Lutrelle F. Palmer well, I had heard an anecdote that did him honor. It also held promise of what has happened to him since. When the white principals of Richmond’s colored high schools were finally displaced by Negroes, Dr. Palmer was offered the principalship of the new Maggie Walker High School. Richmond wanted him badly, but offered him less than the principals of the white high schools were getting. Palmer naturally refused. The dickering continued; larger and larger salaries were offered. Finally Palmer stated his price, which was exactly that being paid white principals. The representative of the Richmond board spoke in aggrieved surprise: “But, Dr. Palmer, you know we can’t pay you that. That’s what we pay white principals.” As the anecdote was told me at Slaughter’s Café in Richmond, “The white folks offered Palmer all the way up to $3,000. ‘Couldn’t you take just $2,998.98, Mr. Palmer? We can’t go a cent higher.’ The white folks were nearly crying. Even called him Mr. Palmer. But Palmer wasn’t coming on that deal. Not for one penny less.” Palmer admitted gravely, a bit stiffly, that the anecdote was to all intents and purposes true. I have known him for many years, having visited his model high school in Newport News, and having served with him on the staff of the Atlanta Summer School where he supervised educational workshops. About the campus he was always earnest, but in the classroom his reserve was shed and a zeal, almost that of a preacher, took its place. Years ago when I was a school kid, around Howard University I had heard him debate for Wilberforce against Howard; I remember him, even after thirty years, quiet but forceful and convincing, a man for Howard to watch. He had grown bald in the meanwhile, but the piercing, almost glaring eyes beneath the heavy rimmed glasses were the same as when he used to say “Honorable Judges, Ladies, and Gentlemen.”