Artykuły w czasopismach: „Multipotency”

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Mrksich, Milan. "Multipotency retained". Nature Materials 10, nr 8 (22.07.2011): 559–60. http://dx.doi.org/10.1038/nmat3086.

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Istiaq, Arif, i Kunimasa Ohta. "Ribosome-Induced Cellular Multipotency, an Emerging Avenue in Cell Fate Reversal". Cells 10, nr 9 (1.09.2021): 2276. http://dx.doi.org/10.3390/cells10092276.

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The ribosome, which is present in all three domains of life, plays a well-established, critical role in the translation process by decoding messenger RNA into protein. Ribosomal proteins, in contrast, appear to play non-translational roles in growth, differentiation, and disease. We recently discovered that ribosomes are involved in reverting cellular potency to a multipotent state. Ribosomal incorporation (the uptake of free ribosome by living cells) can direct the fate of both somatic and cancer cells into multipotency, allowing them to switch cell lineage. During this process, both types of cells experienced cell-cycle arrest and cellular stress while remaining multipotent. This review provides a molecular perspective on current insights into ribosome-induced multipotency and sheds light on how a common stress-associated mechanism may be involved. We also discuss the impact of this phenomenon on cancer cell reprogramming and its potential in cancer therapy.

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Ismail, A. A., S. Wagner, H. Murua Escobar, S. Willenbrock, K. A. Sterenczak, M. T. Samy, A. M. Abd El-Aal, I. Nolte i P. Wefstaedt. "Effects of High-Mobility Group A Protein Application on Canine Adipose-Derived Mesenchymal Stem CellsIn Vitro". Veterinary Medicine International 2012 (2012): 1–10. http://dx.doi.org/10.1155/2012/752083.

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Multipotency and self-renewal are considered as most important features of stem cells to persist throughout life in tissues. In this context, the role of HMGA proteins to influence proliferation of adipose-derived mesenchymal stem cell (ASCs) while maintaining their multipotent and self-renewal capacities has not yet been investigated. Therefore, extracellular HMGA1 and HMGA2 application alone (10–200 ng/mL) and in combination with each other (100, 200 ng/mL each) was investigated with regard to proliferative effects on canine ASCs (cASCs) after 48 hours of cultivation. Furthermore, mRNA expression of multipotency marker genes in unstimulated and HMGA2-stimulated cASCs (50, 100 ng/mL) was analyzed by RT-qPCR. HMGA1 significantly reduced cASCs proliferation in concentrations of 10–200 ng/mL culture medium. A combination of HMGA1 and HMGA2 protein (100 and 200 ng/mL each) caused the same effects, whereas no significant effect on cASCs proliferation was shown after HMGA2 protein application alone. RT-qPCR results showed that expression levels of marker genes including KLF4, SOX2, OCT4, HMGA2, and cMYC mRNAs were on the same level in both HMGA2-protein-stimulated and -unstimulated cASCs. Extracellular HMGA protein application might be valuable to control proliferation of cASCs in context with their employment in regenerative approaches without affecting their self-renewal and multipotency abilities.

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Teng, Chiao-Fang, Long-Bin Jeng i Woei-Cherng Shyu. "Role of Insulin-like Growth Factor 1 Receptor Signaling in Stem Cell Stemness and Therapeutic Efficacy". Cell Transplantation 27, nr 9 (8.06.2018): 1313–19. http://dx.doi.org/10.1177/0963689718779777.

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Evidence has emerged that stem cells represent a promising therapeutic tool for tissue engineering and regenerative medicine. Thus, identifying functional markers for selecting stem cells capable of superior self-renewal and pluripotency (or multipotency) and maintaining stem cell identity under appropriate culture conditions are critical for guiding the use of stem cells toward clinical applications. Many investigations have implicated the insulin-like growth factor 1 receptor (IGF1R) signaling in maintenance of stem cell characteristics and enhancement of stem cell therapy efficacy. IGF1R-expressing stem cells display robust pluripotent or multipotent properties. In this review, we summarize the essential roles of IGF1R signaling in self-renewal, pluripotency (or multipotency), and therapeutic efficacy of stem cells, including human embryonic stem cells, neural stem cells, cardiac stem cells, bone marrow mesenchymal stem cells, placental mesenchymal stem cells, and dental pulp mesenchymal stem cells. Modifying IGF1R signaling may thus provide potential strategies for maintaining stem cell properties and improving stem-cell-based therapeutic applications.

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Miceli, Vitale, Mariangela Pampalone, Serena Vella, Anna Paola Carreca, Giandomenico Amico i Pier Giulio Conaldi. "Comparison of Immunosuppressive and Angiogenic Properties of Human Amnion-Derived Mesenchymal Stem Cells between 2D and 3D Culture Systems". Stem Cells International 2019 (18.02.2019): 1–16. http://dx.doi.org/10.1155/2019/7486279.

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The secretion of potential therapeutic factors by mesenchymal stem cells (MSCs) has aroused much interest given the benefits that it can bring in the field of regenerative medicine. Indeed, the in vitro multipotency of these cells and the secretive capacity of both angiogenic and immunomodulatory factors suggest a role in tissue repair and regeneration. However, during culture, MSCs rapidly lose the expression of key transcription factors associated with multipotency and self-renewal, as well as the ability to produce functional paracrine factors. In our study, we show that a three-dimensional (3D) culture method is effective to induce MSC spheroid formation, to maintain the multipotency and to improve the paracrine activity of a specific population of human amnion-derived MSCs (hAMSCs). The regenerative potential of both 3D culture-derived conditioned medium (3D CM) and their exosomes (EXO) was assessed against 2D culture products. In particular, tubulogenesis assays revealed increased capillary maturation in the presence of 3D CM compared with both 2D CM and 2D EXO. Furthermore, 3D CM had a greater effect on inhibition of PBMC proliferation than both 2D CM and 2D EXO. To support this data, hAMSC spheroids kept in our 3D culture system remained viable and multipotent and secreted considerable amounts of both angiogenic and immunosuppressive factors, which were detected at lower levels in 2D cultures. This work reveals the placenta as an important source of MSCs that can be used for eventual clinical applications as cell-free therapies.

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Juliano, C. E., S. Z. Swartz i G. M. Wessel. "A conserved germline multipotency program". Development 137, nr 24 (23.11.2010): 4113–26. http://dx.doi.org/10.1242/dev.047969.

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Xie, Meng, Dmitrii Kamenev, Marketa Kaucka, Maria Eleni Kastriti, Baoyi Zhou, Artem V. Artemov, Mekayla Storer i in. "Schwann cell precursors contribute to skeletal formation during embryonic development in mice and zebrafish". Proceedings of the National Academy of Sciences 116, nr 30 (8.07.2019): 15068–73. http://dx.doi.org/10.1073/pnas.1900038116.

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Immature multipotent embryonic peripheral glial cells, the Schwann cell precursors (SCPs), differentiate into melanocytes, parasympathetic neurons, chromaffin cells, and dental mesenchymal populations. Here, genetic lineage tracing revealed that, during murine embryonic development, some SCPs detach from nerve fibers to become mesenchymal cells, which differentiate further into chondrocytes and mature osteocytes. This occurred only during embryonic development, producing numerous craniofacial and trunk skeletal elements, without contributing to development of the appendicular skeleton. Formation of chondrocytes from SCPs also occurred in zebrafish, indicating evolutionary conservation. Our findings reveal multipotency of SCPs, providing a developmental link between the nervous system and skeleton.

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Malvicini, Ricardo, Diego Santa-Cruz, Natalia Pacienza i Gustavo Yannarelli. "OCT4 Silencing Triggers Its Epigenetic Repression and Impairs the Osteogenic and Adipogenic Differentiation of Mesenchymal Stromal Cells". International Journal of Molecular Sciences 20, nr 13 (3.07.2019): 3268. http://dx.doi.org/10.3390/ijms20133268.

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Mechanisms mediating mesenchymal stromal/stem cells’ (MSCs) multipotency are unclear. Although the expression of the pluripotency factor OCT4 has been detected in MSCs, whether it has a functional role in adult stem cells is still controversial. We hypothesized that a physiological expression level of OCT4 is important to regulate MSCs’ multipotency and trigger differentiation in response to environmental signals. Here, we specifically suppressed OCT4 in MSCs by using siRNA technology before directed differentiation. OCT4 expression levels were reduced by 82% in siOCT4-MSCs, compared with controls. Interestingly, siOCT4-MSCs also presented a hypermethylated OCT4 promoter. OCT4 silencing significantly impaired the ability of MSCs to differentiate into osteoblasts. Histologic and macroscopic analysis showed a lower degree of mineralization in siOCT4-MSCs than in controls. Moreover, OCT4 silencing prevented the up-regulation of osteoblast lineage-associated genes during differentiation. Similarly, OCT4 silencing resulted in decreased MSC differentiation potential towards the adipogenic lineage. The accumulation of lipids was reduced 3.0-fold in siOCT4-MSCs, compared with controls. The up-regulation of genes engaged in the early stages of adipogenesis was also suppressed in siOCT4-MSCs. Our findings provide evidence of a functional role for OCT4 in MSCs and indicate that a basal expression of this transcription factor is essential for their multipotent capacity.

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Swartz, S. Zachary, Celina E. Juliano, Tal Raz, Doron Lipson, Patrice Milos, Amro Hamdoun i Gary M. Wessel. "An ancient molecular circuit specifying multipotency". Developmental Biology 344, nr 1 (sierpień 2010): 514–15. http://dx.doi.org/10.1016/j.ydbio.2010.05.368.

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Rahnama, Ruyan, i Challice L. Bonifant. "Engineering builds multipotency for iPSC-NKs". Blood 140, nr 23 (8.12.2022): 2414–16. http://dx.doi.org/10.1182/blood.2022017794.

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Scerbo, Pierluigi, i Anne H. Monsoro-Burq. "The vertebrate-specific VENTX/NANOG gene empowers neural crest with ectomesenchyme potential". Science Advances 6, nr 18 (29.04.2020): eaaz1469. http://dx.doi.org/10.1126/sciadv.aaz1469.

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During Cambrian, unipotent progenitors located at the neural (plate) border (NB) of an Olfactoria chordate embryo acquired the competence to form ectomesenchyme, pigment cells and neurons, initiating the rise of the multipotent neural crest cells (NC) specific to vertebrates. Surprisingly, the known vertebrate NB/NC transcriptional circuitry is a constrained feature also found in invertebrates. Therefore, evidence for vertebrate-specific innovations endowing vertebrate NC with multipotency is still missing. Here, we identified VENTX/NANOG and POU5/OCT4 as vertebrate-specific innovations. When VENTX was depleted in vivo and in directly-induced NC, the NC lost its early multipotent state and its skeletogenic potential, but kept sensory neuron and pigment identity, thus reminiscent of invertebrate NB precursors. In vivo, VENTX gain-of-function enabled NB specifiers to reprogram embryonic non-neural ectoderm towards early NC identity. We propose that skeletogenic NC evolved by acquiring VENTX/NANOG activity, promoting a novel multipotent progenitor regulatory state into the pre-existing sensory neuron/pigment NB program.

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Yu. V. Poliachenko, E. M. Zapol’s’ka i R. V. Saliutin. "Directed differentiation of stem cells that are isolated from the adipose tissue". Bukovinian Medical Herald 17, nr 1 (65) (2.02.2013): 92–95. http://dx.doi.org/10.24061/2413-0737.xvii.1.65.2013.23.

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The authors have carried out an experimental study for the purpose of determining a possibility of differentiating stem cells that are isolated from the adipose tissue according to the adipogenic orientation. The results of the research were indicative of the fact that the cells isolated from the adipose tissue are of specific differentiation, namely, an osteogenic, chondrogenic and, above all, adipogenic orientation that is an evidence of the multipotency of the stem mesenchymal cells of the adipose tissue. The adipose tissue is an alternative source to the bone marrow of multipotent mesenchymal stem cells that can be used as a protection of transplanted autologous adipose tissue from resorption by lipofilling.

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Marycz, K., J. M. Irwin Houston, C. Weiss, M. Röcken i K. Kornicka. "5-Azacytidine and Resveratrol Enhance Chondrogenic Differentiation of Metabolic Syndrome-Derived Mesenchymal Stem Cells by Modulating Autophagy". Oxidative Medicine and Cellular Longevity 2019 (12.05.2019): 1–20. http://dx.doi.org/10.1155/2019/1523140.

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Recently, metabolic syndrome (MS) has gained attention in human and animal metabolic medicine. Insulin resistance, inflammation, hyperleptinemia, and hyperinsulinemia are critical to its definition. MS is a complex cluster of metabolic risk factors that together exert a wide range of effects on multiple organs, tissues, and cells in the body. Adipose stem cells (ASCs) are multipotent stem cell population residing within the adipose tissue that is inflamed during MS. Studies have indicated that these cells lose their stemness and multipotency during MS, which strongly reduces their therapeutic potential. They suffer from oxidative stress, apoptosis, and mitochondrial deterioration. Thus, the aim of this study was to rejuvenate these cells in vitro in order to improve their chondrogenic differentiation effectiveness. Pharmacotherapy of ASCs was based on resveratrol and 5-azacytidine pretreatment. We evaluated whether those substances are able to reverse aged phenotype of metabolic syndrome-derived ASCs and improve their chondrogenic differentiation at its early stage using immunofluorescence, transmission and scanning electron microscopy, real-time PCR, and flow cytometry. Obtained results indicated that 5-azacytidine and resveratrol modulated mitochondrial dynamics, autophagy, and ER stress, leading to the enhancement of chondrogenesis in metabolically impaired ASCs. Therefore, pretreatment of these cells with 5-azacytidine and resveratrol may become a necessary intervention before clinical application of these cells in order to strengthen their multipotency and therapeutic potential.

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Youssef, Amer, i Victor K. M. Han. "Regulation of Osteogenic Differentiation of Placental-Derived Mesenchymal Stem Cells by Insulin-Like Growth Factors and Low Oxygen Tension". Stem Cells International 2017 (2017): 1–17. http://dx.doi.org/10.1155/2017/4576327.

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Placental mesenchymal stem cells (PMSCs) are multipotent cells that can differentiate in vitro to multiple lineages, including bone. Insulin-like growth factors (IGFs, IGF-1 and IGF-2) participate in maintaining growth, survival, and differentiation of many stem cells, including osteoprogenitors. Low oxygen tension (PO2) can maintain stem cell multipotency and impede osteogenic differentiation. In this study, we investigated whether PMSC osteogenic differentiation is influenced by low PO2 and by IGFs. Our results indicated that low PO2 decreased osteogenic markers RUNX2 and OPN; however, re-exposure to higher oxygen tension (room air) restored differentiation. IGFs, especially IGF-1, triggered an earlier expression of RUNX2 and enhanced OPN and mineralization. RUNX2 was phosphorylated in room air and augmented by IGFs. IGF-1 receptor (IGF-1R) was increased in low PO2 and reduced by IGFs, while insulin receptor (IR) was increased in differentiating PMSCs and enhanced by IGF-1. Low PO2 and IGFs maintained higher IR-A which was switched to IR-B in room air. PI3K/AKT was required for osteogenic differentiation, while MEK/ERK was required to repress an RUNX2 and OPN increase in low PO2. Therefore, IGFs, specifically IGF-1, trigger the earlier onset of osteogenic differentiation in room air, whereas, reversibly, low PO2 impedes complete differentiation by maintaining higher multipotency and lower differentiation markers.

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Sakai, V. T., M. M. Cordeiro, Z. Dong, Z. Zhang, B. D. Zeitlin i J. E. Nör. "Tooth Slice/Scaffold Model of Dental Pulp Tissue Engineering". Advances in Dental Research 23, nr 3 (15.06.2011): 325–32. http://dx.doi.org/10.1177/0022034511405325.

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Multipotency is a defining characteristic of post-natal stem cells. The human dental pulp contains a small subpopulation of stem cells that exhibit multipotency, as demonstrated by their ability to differentiate into odontoblasts, neural cells, and vascular endothelial cells. These discoveries highlight the fundamental role of stem cells in the biology of the dental pulp and suggest that these cells are uniquely suited for dental pulp tissue-engineering purposes. The availability of experimental approaches specifically designed for studies of the differentiation potential of dental pulp stem cells has played an important role in these discoveries. The objective of this review is to describe the development and characterization of the Tooth Slice/Scaffold Model of Dental Pulp Tissue Engineering. In addition, we discuss the multipotency of dental pulp stem cells, focusing on the differentiation of these cells into functional odontoblasts and into vascular endothelial cells.

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Youssef, Amer, i Victor K. M. Han. "Low Oxygen Tension Modulates the Insulin-Like Growth Factor-1 or -2 Signaling via Both Insulin-Like Growth Factor-1 Receptor and Insulin Receptor to Maintain Stem Cell Identity in Placental Mesenchymal Stem Cells". Endocrinology 157, nr 3 (13.01.2016): 1163–74. http://dx.doi.org/10.1210/en.2015-1297.

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Abstract Placental mesenchymal stem cells (PMSCs) are readily available multipotent stem cells for potential use in regenerative therapies. For this purpose, PMSCs must be maintained in culture conditions that mimic the in vivo microenvironment. IGFs (IGF-1 and IGF-2) and oxygen tension are low in the placenta in early gestation and increase as pregnancy progresses. IGFs bind to two receptor tyrosine kinases, the IGF-1 receptor (IGF-1R) and the insulin receptor (IR), and their hybrid receptors. We hypothesized that IGF-1 and IGF-2 signal via distinct signaling pathways under low-oxygen tension to maintain PMSC multipotency. In preterm PMSCs, low-oxygen tension increased the expression of IGF-2 and reduced IGF-1. IGF-1 stimulated higher phosphorylation of IGF-1Rβ, ERK1/2, and AKT, which was maintained at steady lower levels by low oxygen tension. PMSC proliferation was increased by IGF-1 more than IGF-2,and was potentiated by low-oxygen tension. This IGF/low oxygen tension-mediated proliferation was receptor dependent because neutralization of the IGF-1R inhibited PMSC proliferation in the presence of IGF-1 and the IR in presence of IGF-2. These findings suggest that both IGF-1R and the IR can participate in mediating IGF signaling in maintaining PMSCs multipotency. We conclude that low-oxygen tension can modify the IGF-1 or IGF-2 signaling via the IGF-1R and IR in PMSCs.

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Fangping, Chen, i Huarong Tang. "Characterization of Histone Modifications on Lineage-Affiliated Genes During Hematopoietic Stem Cell Differentiation". Blood 116, nr 21 (19.11.2010): 4784. http://dx.doi.org/10.1182/blood.v116.21.4784.4784.

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Abstract Abstract 4784 Hematopoietic stem cells (HSCs) are multipotent stem cells capable of self-renewal and multi-lineage differentiation. Though it has been shown that multiple factors take part in the maintenance of HSCs’ multipotency and differentiation potential, the mechanisms are unclear. Recent studies showed that histone modifications play an important role in maintenance of embryonic stem cells pluripotency and differentiation. To characterize the histone modification patterns of different lineages, HSCs were collected from umbilical cord blood and induced to differentiate to granulocytic, erythroid, and megakarytic in vitro. genes during HSC differentiation. Chromatin immunoprecipitation-quantitative PCR (ChIP-qPCR) technology was adopted to investigate the dynamic changes of histone modifications on lineage specific transcription factors and lineage–affiliated genes. Our results showed a certain level of H4 acetylation and H3 acetylation together with high level of H3K4me2 and low level of H3K4me3, H3K9me3 and H3K27me3 were present in lineage specific genes in CD34+CD38- HSCs. As CD34+CD38- cells differentiated, the modification level of acH3, acH4, H3K4me2, H3K9me3 and H3K27me3 on lineage specific genes remained the same, while H3K4me3 level increased greatly. In non-lineage specific genes, the acH3 and acH4 levels decreased, and H3K4me3 level remain at low level, while H3K9me3 and H3K27me3 levels increased. Thus, our data suggested that histone modifications played an important role in maintenaning the multipotency and differentiation capability of hematopoietic stem cells. Disclosures: No relevant conflicts of interest to declare.

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Vo, Linda T., Melissa A. Kinney, Xin Liu, Yuannyu Zhang, Jessica Barragan, Patricia M. Sousa, Deepak K. Jha i in. "Regulation of embryonic haematopoietic multipotency by EZH1". Nature 553, nr 7689 (styczeń 2018): 506–10. http://dx.doi.org/10.1038/nature25435.

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Tika, Elisavet, Marielle Ousset, Anne Dannau i Cédric Blanpain. "Spatiotemporal regulation of multipotency during prostate development". Development 146, nr 20 (1.10.2019): dev180224. http://dx.doi.org/10.1242/dev.180224.

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Shimada, Shin, Satoshi Nunomura, Shuya Mori, Hiroshi Suemizu, Toshio Itoh, Shuji Takabayashi, Yoshinori Okada i in. "Common marmoset CD117+hematopoietic cells possess multipotency". International Immunology 27, nr 11 (14.05.2015): 567–77. http://dx.doi.org/10.1093/intimm/dxv031.

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Purnell, Beverly A. "Notch role in multipotency or cell fate". Science Signaling 8, nr 404 (24.11.2015): ec353-ec353. http://dx.doi.org/10.1126/scisignal.aad9094.

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Purnell, B. A. "Notch role in multipotency or cell fate". Science 350, nr 6263 (19.11.2015): 923–25. http://dx.doi.org/10.1126/science.350.6263.923-k.

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Valny, Martin, Pavel Honsa, Jan Kriska i Miroslava Anderova. "Multipotency and therapeutic potential of NG2 cells". Biochemical Pharmacology 141 (październik 2017): 42–55. http://dx.doi.org/10.1016/j.bcp.2017.05.008.

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Ji, Yu, Hongyan Hao, Kurt Reynolds, Moira McMahon i Chengji J. Zhou. "Wnt Signaling in Neural Crest Ontogenesis and Oncogenesis". Cells 8, nr 10 (29.09.2019): 1173. http://dx.doi.org/10.3390/cells8101173.

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Neural crest (NC) cells are a temporary population of multipotent stem cells that generate a diverse array of cell types, including craniofacial bone and cartilage, smooth muscle cells, melanocytes, and peripheral neurons and glia during embryonic development. Defective neural crest development can cause severe and common structural birth defects, such as craniofacial anomalies and congenital heart disease. In the early vertebrate embryos, NC cells emerge from the dorsal edge of the neural tube during neurulation and then migrate extensively throughout the anterior-posterior body axis to generate numerous derivatives. Wnt signaling plays essential roles in embryonic development and cancer. This review summarizes current understanding of Wnt signaling in NC cell induction, delamination, migration, multipotency, and fate determination, as well as in NC-derived cancers.

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Sherman, Lauren S., Nicholas M. Ponzio i Pranela Rameshwar. "Understanding the effects of an inflammatory milieu on the development of mesenchymal stem cells: implication for treating inflammatory diseases". Journal of Immunology 196, nr 1_Supplement (1.05.2016): 140.25. http://dx.doi.org/10.4049/jimmunol.196.supp.140.25.

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Abstract Mesenchymal stem cells (MSCs), multipotent cells found in various adult tissues, are an attractive source of cells for cellular therapy and regenerative medicine. Reasons include their ease to expand, plasticity to generate cells of all germ layers, reduced ethical concerns, and ability to be available as ‘off the shelf’ cells for immediate use in transplantation. MSCs can respond differently to varying microenvironments to perform distinct immune functions. The microenvironment can also affect the developmental state of MSCs. Better understanding of how the microenvironment influences MSC multipotency is crucial for effective translational use of these cells in the clinic. This study tested the hypothesis that the changes in an inflammatory microenvironment will influence MSC function. To study these effects, an in vitro model of GvHD was developed by culturing MSCs in a three-dimensional model – rather than in a traditional monolayer – under physiological conditions. The GvHD model was generated based on a modified two-way mixed lymphocyte reaction. The cells were then assessed for phenotype, proliferation, and viability. Following growth in suspension, the MSCs continued to express an MSC phenotype. Proliferation and viability studies suggested that, although the cells were proliferating, they underwent cell death at a quicker rate than their two-dimensional culture counterparts. When cultured in the GvHD model, MSCs exhibited increased viability as compared to culture in the three-dimensional system alone. This in vitro GvHD model will elucidate factors within the inflammatory milieu that alter MSC multipotency. Identifying these factors will allow for more controlled and effective clinical use of MSCs.

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Ma, Yujie, Martin P. Neubauer, Julian Thiele, Andreas Fery i W. T. S. Huck. "Artificial microniches for probing mesenchymal stem cell fate in 3D". Biomater. Sci. 2, nr 11 (2014): 1661–71. http://dx.doi.org/10.1039/c4bm00104d.

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Sun, Dayu, Linji Gong, Jing Xie, Xiao He, Siyu Chen, Luodan A, Qiyou Li, Zhanjun Gu i Haiwei Xu. "Evaluating the toxicity of silicon dioxide nanoparticles on neural stem cells using RNA-Seq". RSC Adv. 7, nr 75 (2017): 47552–64. http://dx.doi.org/10.1039/c7ra09512k.

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Hyder, Ayman, Sabrina Ehnert, Fred Fändrich i Hendrik Ungefroren. "Transfection of Peripheral Blood Monocytes withSOX2Enhances Multipotency, Proliferation, and Redifferentiation into Neohepatocytes and Insulin-Producing Cells". Stem Cells International 2018 (4.10.2018): 1–8. http://dx.doi.org/10.1155/2018/4271875.

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Following a several-day incubation in medium containing IL-3 and M-CSF to generate a more plastic intermediate “reprogrammed multipotent cells of monocytic origin (RMCMO),” peripheral blood mononuclear cells (PBMCs) can be efficiently converted to hepatocyte-like cells (neohepatocytes) and insulin-producing cells. However, continuous efforts are devoted to enhancing the proliferative capacity of these multipotent cells while maintaining or further increasing their redifferentiation potential. In the present work, PBMCs were transfected with one pluripotency gene (SOX2) and the resulting RMCMO compared to standard RMCMO with respect to cell viability, proliferative activity, and redifferentiation potential. EctopicSOX2expression increased the number of viable RMCMO, activated cell cycle genes, and enhanced proliferation as shown by quantitative RT-PCR and Ki67 immunofluorescent staining, respectively. Redifferentiation of RMCMO derived fromSOX2-transfected PBMCs to neohepatocytes was more complete in comparison to control cells as revealed by higher urea and glucose secretion, increased activity of cytochrome P450 isoforms, and a phase II enzyme, while the same was true for insulin-producing cells as assessed by the expression ofINS,PDX1, andGLUT2and glucose-stimulated insulin secretion. Our results indicate thatSOX2transfection increases both multipotency and proliferation of RMCMO, eventually allowing production of neohepatocytes and insulin-producing cells of higher quality and quantity for transplantation purposes.

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Fu, Nai Yang, Emma Nolan, Geoffrey J. Lindeman i Jane E. Visvader. "Stem Cells and the Differentiation Hierarchy in Mammary Gland Development". Physiological Reviews 100, nr 2 (1.04.2020): 489–523. http://dx.doi.org/10.1152/physrev.00040.2018.

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The mammary gland is a highly dynamic organ that undergoes profound changes within its epithelium during puberty and the reproductive cycle. These changes are fueled by dedicated stem and progenitor cells. Both short- and long-lived lineage-restricted progenitors have been identified in adult tissue as well as a small pool of multipotent mammary stem cells (MaSCs), reflecting intrinsic complexity within the epithelial hierarchy. While unipotent progenitor cells predominantly execute day-to-day homeostasis and postnatal morphogenesis during puberty and pregnancy, multipotent MaSCs have been implicated in coordinating alveologenesis and long-term ductal maintenance. Nonetheless, the multipotency of stem cells in the adult remains controversial. The advent of large-scale single-cell molecular profiling has revealed striking changes in the gene expression landscape through ontogeny and the presence of transient intermediate populations. An increasing number of lineage cell-fate determination factors and potential niche regulators have now been mapped along the hierarchy, with many implicated in breast carcinogenesis. The emerging diversity among stem and progenitor populations of the mammary epithelium is likely to underpin the heterogeneity that characterizes breast cancer.

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Bellagamba, Bruno Corrêa, Patrícia Bencke Grudzinski, Pedro Bins Ely, Paulo de Jesus Hartmann Nader, Nance Beyer Nardi i Lindolfo da Silva Meirelles. "Induction of Expression of CD271 and CD34 in Mesenchymal Stromal Cells Cultured as Spheroids". Stem Cells International 2018 (31.07.2018): 1–14. http://dx.doi.org/10.1155/2018/7357213.

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Cultured mesenchymal stromal cells (MSCs) are cells that can be used for tissue engineering or cell therapies owing to their multipotency and ability to secrete immunomodulatory and trophic molecules. Several studies suggest that MSCs can become pericytes when cocultured with endothelial cells (ECs) but failed to use pericyte markers not already expressed by MSCs. We hypothesized ECs could instruct MSCs to express the molecules CD271 or CD34, which are expressed by pericytes in situ but not by MSCs. CD271 is a marker of especial interest because it is associated with multipotency, a characteristic that wanes in MSCs as they are culture expanded. Consequently, surface expression of CD271 and CD34 was detected in roughly half of the MSCs cocultured with ECs as spheroids in the presence of insulin-like growth factor 1 (IGF-1). Conversely, expression of CD271 and CD34 was detected in a similar proportion of MSCs cultured under these conditions without ECs, and expression of these markers was low or absent when no IGF-1 was added. These findings indicate that specific culture conditions including IGF-1 can endow cultured MSCs with expression of CD271 and CD34, which may enhance the multipotency of these cells when they are used for therapeutic purposes.

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Motohashi, Tsutomu, Katsumasa Yamanaka, Kairi Chiba, Hitomi Aoki i Takahiro Kunisada. "Unexpected Multipotency of Melanoblasts Isolated from Murine Skin". STEM CELLS 27, nr 4 (kwiecień 2009): 888–97. http://dx.doi.org/10.1634/stemcells.2008-0678.

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GAO, Liang, Li CAO, Zhi-da SU, Yan-ling ZHU i Cheng HE. "Multipotency of cultured olfactory epithelium neural stem cells". Academic Journal of Second Military Medical University 29, nr 9 (30.12.2009): 985–89. http://dx.doi.org/10.3724/sp.j.1008.2009.00985.

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Berdasco, María, i Manel Esteller. "DNA methylation in stem cell renewal and multipotency". Stem Cell Research & Therapy 2, nr 5 (2011): 42. http://dx.doi.org/10.1186/scrt83.

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Okkenhaug, Klaus, i Rahul Roychoudhuri. "Oncogenic PI3Kα promotes multipotency in breast epithelial cells". Science Signaling 8, nr 401 (3.11.2015): pe3. http://dx.doi.org/10.1126/scisignal.aad5856.

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Koren, Shany, Linsey Reavie, Joana Pinto Couto, Duvini De Silva, Michael B. Stadler, Tim Roloff, Adrian Britschgi i in. "PIK3CAH1047R induces multipotency and multi-lineage mammary tumours". Nature 525, nr 7567 (12.08.2015): 114–18. http://dx.doi.org/10.1038/nature14669.

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MURATA, Daiki, Atsushi YAMASAKI, Shouta MATSUZAKI, Takafumi SUNAGA, Makoto FUJIKI, Satoshi TOKUNAGA i Kazuhiro MISUMI. "Characteristics and multipotency of equine dedifferentiated fat cells". Journal of Equine Science 27, nr 2 (2016): 57–65. http://dx.doi.org/10.1294/jes.27.57.

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Mundell, Nathan A., Audrey Y. Frist i Patricia A. Labosky. "Foxd3 regulates neural crest multipotency and self-renewal". Developmental Biology 331, nr 2 (lipiec 2009): 394. http://dx.doi.org/10.1016/j.ydbio.2009.05.038.

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Centanin, Lázaro, Burkhard Hoeckendorf i Joachim Wittbrodt. "Fate Restriction and Multipotency in Retinal Stem Cells". Cell Stem Cell 9, nr 6 (grudzień 2011): 553–62. http://dx.doi.org/10.1016/j.stem.2011.11.004.

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Kusaba, T., i B. D. Humphreys. "Reply to Corbeil et al.: Dedifferentiation and multipotency". Proceedings of the National Academy of Sciences 111, nr 15 (20.03.2014): E1453. http://dx.doi.org/10.1073/pnas.1401733111.

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Casarosa, Simona, Marcos A. Amato, Massimiliano Andreazzoli, Gaia Gestri, Giuseppina Barsacchi i Federico Cremisi. "Xrx1 controls proliferation and multipotency of retinal progenitors". Molecular and Cellular Neuroscience 22, nr 1 (styczeń 2003): 25–36. http://dx.doi.org/10.1016/s1044-7431(02)00025-8.

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Amici, Alessandro Wataru, Masayuki Yamato, Teruo Okano i Koji Kobayashi. "The multipotency of adult vibrissa follicle stem cells". Differentiation 77, nr 3 (marzec 2009): 317–23. http://dx.doi.org/10.1016/j.diff.2008.10.010.

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Kudlik, Gyöngyi, Zsolt Matula, Tamás Kovács, S. Veronika Urbán i Ferenc Uher. "A pluri- és multipotencia határán: a ganglionléc őssejtjei". Orvosi Hetilap 156, nr 42 (październik 2015): 1683–94. http://dx.doi.org/10.1556/650.2015.30271.

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The neural crest is a transient, multipotent, migratory cell population that is unique to vertebrate embryos and gives rise to many derivatives, ranging from the neuronal and glial components of the peripheral nervous system to the ectomesenchymal derivatives of the craniofacial area and pigment cells in the skin. Intriguingly, the neural crest derived stem cells are not only present in the embryonic neural crest, but also in their target tissues in the fetus and adult. These postmigratory stem cells, at least partially, resemble their multipotency. Moreover, fully differentiated neural crest-derived cells such as Schwann cells and melanocytes are able to dedifferentiate into stem-like progenitors. Here the authors review current understanding of this unique plasticity and its potential application in stem cell biology as well as in regenerative medicine. Orv. Hetil., 2015, 156(42), 1683–1694.

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Algorta, Agustina, Rody Artigas, Analía Rial, Scott Brandl, Clementina Rodellar, Uruguaysito Benavides, Jacqueline Maisonnave i Kevin Yaneselli. "Isolation and characterization of feline dental pulp stem cells". Journal of Feline Medicine and Surgery 25, nr 2 (luty 2023): 1098612X2211506. http://dx.doi.org/10.1177/1098612x221150625.

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Objectives The aim of this study was to isolate feline dental pulp stem cells (fDPSCs) and characterize their clonogenic and proliferative abilities, as well as their multipotency, immunophenotype and cytogenetic stability. Methods Dental pulp was isolated by explant culture from two cats <1 year old at post mortem. Their clonogenicity was characterized using a colony-forming unit fibroblast assay, and their proliferative ability was quantified with a doubling time assay in passages 2, 4 and 6 (P2, P4 and P6, respectively). Multipotency was characterized with an in vitro trilineage differentiation assay in P2, and cells were immunophenotyped in P4 by flow cytometry. Chromosomic stability was evaluated by cytogenetic analysis in P2, P4 and P6. Results The fDPSCs displayed spindle and epithelial-like morphologies. Isolated cells showed a marked clonogenic capacity and doubling time was maintained from P2 to P6. Trilineage differentiation was obtained in one sample, while the other showed osteogenic and chondrogenic differentiation. Immunophenotypic analysis showed fDPSCs were CD45−, CD90+ and CD44+. Structural and numerical cytogenetic aberrations were observed in P2–P4. Conclusions and relevance In this study, fDPSCs from two cats were isolated by explant culture and immunophenotyped. Cells displayed clonogenic and proliferative ability, and multipotency in vitro, and signs of chromosomic instability were observed. Although a larger study is needed to confirm these results, this is the first report of fDPSC isolation and in vitro characterization.

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Vodnala, Suman Kumar, Robert Eil, Rigel J. Kishton, Madhusudhanan Sukumar, Tori N. Yamamoto, Ngoc-Han Ha, Ping-Hsien Lee i in. "T cell stemness and dysfunction in tumors are triggered by a common mechanism". Science 363, nr 6434 (28.03.2019): eaau0135. http://dx.doi.org/10.1126/science.aau0135.

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A paradox of tumor immunology is that tumor-infiltrating lymphocytes are dysfunctional in situ, yet are capable of stem cell–like behavior including self-renewal, expansion, and multipotency, resulting in the eradication of large metastatic tumors. We find that the overabundance of potassium in the tumor microenvironment underlies this dichotomy, triggering suppression of T cell effector function while preserving stemness. High levels of extracellular potassium constrain T cell effector programs by limiting nutrient uptake, thereby inducing autophagy and reduction of histone acetylation at effector and exhaustion loci, which in turn produces CD8+ T cells with improved in vivo persistence, multipotency, and tumor clearance. This mechanistic knowledge advances our understanding of T cell dysfunction and may lead to novel approaches that enable the development of enhanced T cell strategies for cancer immunotherapy.

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Loughran, Stephen J., Federico Comoglio, Fiona K. Hamey, Alice Giustacchini, Youssef Errami, Eleanor Earp, Berthold Göttgens i in. "Mbd3/NuRD controls lymphoid cell fate and inhibits tumorigenesis by repressing a B cell transcriptional program". Journal of Experimental Medicine 214, nr 10 (12.09.2017): 3085–104. http://dx.doi.org/10.1084/jem.20161827.

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Differentiation of lineage-committed cells from multipotent progenitors requires the establishment of accessible chromatin at lineage-specific transcriptional enhancers and promoters, which is mediated by pioneer transcription factors that recruit activating chromatin remodeling complexes. Here we show that the Mbd3/nucleosome remodeling and deacetylation (NuRD) chromatin remodeling complex opposes this transcriptional pioneering during B cell programming of multipotent lymphoid progenitors by restricting chromatin accessibility at B cell enhancers and promoters. Mbd3/NuRD-deficient lymphoid progenitors therefore prematurely activate a B cell transcriptional program and are biased toward overproduction of pro–B cells at the expense of T cell progenitors. The striking reduction in early thymic T cell progenitors results in compensatory hyperproliferation of immature thymocytes and development of T cell lymphoma. Our results reveal that Mbd3/NuRD can regulate multilineage differentiation by constraining the activation of dormant lineage-specific enhancers and promoters. In this way, Mbd3/NuRD protects the multipotency of lymphoid progenitors, preventing B cell–programming transcription factors from prematurely enacting lineage commitment. Mbd3/NuRD therefore controls the fate of lymphoid progenitors, ensuring appropriate production of lineage-committed progeny and suppressing tumor formation.

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Singh, Rabindra P., Ying-Hua Cheng, Paul Nelson i Feng C. Zhou. "Retentive Multipotency of Adult Dorsal Root Ganglia Stem Cells". Cell Transplantation 18, nr 1 (styczeń 2009): 55–68. http://dx.doi.org/10.3727/096368909788237177.

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Djouad, Farida, Wesley M. Jackson, Brent E. Bobick, Sasa Janjanin, Yingjie Song, George TJ Huang i Rocky S. Tuan. "Activin A expression regulates multipotency of mesenchymal progenitor cells". Stem Cell Research & Therapy 1, nr 2 (2010): 11. http://dx.doi.org/10.1186/scrt11.

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David, Charles N. "Interstitial stem cells in Hydra: multipotency and decision-making". International Journal of Developmental Biology 56, nr 6-7-8 (2012): 489–97. http://dx.doi.org/10.1387/ijdb.113476cd.

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Li, Yang, Xiao-chun Chi, Xiao-xia Li i Jin-chong Xu. "Multipotency of human neural stem cells from fetal striatum". NeuroReport 19, nr 17 (listopad 2008): 1679–83. http://dx.doi.org/10.1097/wnr.0b013e32831576e3.

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Centonze, Alessia, Shuheng Lin, Elisavet Tika, Alejandro Sifrim, Marco Fioramonti, Milan Malfait, Yura Song i in. "Heterotypic cell–cell communication regulates glandular stem cell multipotency". Nature 584, nr 7822 (26.08.2020): 608–13. http://dx.doi.org/10.1038/s41586-020-2632-y.

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