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Wetzig, Andrew R., i n/a. "Olfactory Stem Cells From Adult Rats". Griffith University. School of Biomolecular and Biomedical Science, 2007. http://www4.gu.edu.au:8080/adt-root/public/adt-QGU20070724.121953.
Pełny tekst źródłaWetzig, Andrew R. "Olfactory Stem Cells From Adult Rats". Thesis, Griffith University, 2007. http://hdl.handle.net/10072/366121.
Pełny tekst źródłaThesis (PhD Doctorate)
Doctor of Philosophy (PhD)
School of Biomolecular and Biomedical Sciences
Faculty of Science
Full Text
Wuidart, Aline. "Defining the mechanisms regulating the switch from multipotency to unipotency during mammary gland development". Doctoral thesis, Universite Libre de Bruxelles, 2018. http://hdl.handle.net/2013/ULB-DIPOT:oai:dipot.ulb.ac.be:2013/264618.
Pełny tekst źródłaDoctorat en Sciences biomédicales et pharmaceutiques (Médecine)
info:eu-repo/semantics/nonPublished
McMurray, Rebecca Jane. "Nanoscale surfaces for the long-term maintenance of mesenchymal stem cell phenotype and multipotency". Thesis, University of Glasgow, 2011. http://theses.gla.ac.uk/3042/.
Pełny tekst źródłaGray, Simon Matthew. "PRC2-Mediated H3K27me3 Repression Promotes Effector CD8 + T Cell Terminal Differentiation and Loss of Multipotency". Thesis, Yale University, 2017. http://pqdtopen.proquest.com/#viewpdf?dispub=10584947.
Pełny tekst źródłaElucidating how multipotent memory precursor (MP) cells maintain developmental plasticity and longevity to provide long-term immunity while most effector cells develop into terminally differentiated effector (TE) cells with limited survival is necessary for understanding immunological memory formation. We profiled active (H3K27Ac) and repressed (H3K27me3) chromatin states in naïve, MP and TE CD8+ T cells during viral infection, and observed increased H3K27me3 at numerous pro-memory and pro-survival genes in TE relative to MP cells, indicative of fate restriction, but permissive chromatin at both pro-memory and pro-effector genes in MP cells, indicative of multipotency. Deficiency in PRC2-mediated H3K27me3 deposition impaired clonal expansion and TE cell differentiation, but minimally impacted memory cell maturation. Abundant H3K27me3 deposition at pro-memory genes occurred relatively late during TE cell development in a FOXO1-regulated manner. These results outline a detailed temporal model for how effector T cells lose memory cell potential through selective epigenetic-silencing of pro-memory genes.
Cwinn, Matthew. "'Suppressor of fused' antagonizes hedgehog signaling and is required to maintain retinal progenitor cell identity and multipotency". Thesis, University of Ottawa (Canada), 2009. http://hdl.handle.net/10393/28214.
Pełny tekst źródłaChang, Eun Hyuk. "The role of polycomb repressive complex 2 in postnatal subventricular zone neural stem/progenitor cell self-renewal and multipotency". Thesis, University of Oxford, 2012. http://ora.ox.ac.uk/objects/uuid:1ddbd108-0256-4a4a-b40a-35818197ca39.
Pełny tekst źródłaKole, Denis. "Role of Fibroblast Growth Factor 2 in Maintenance of Multipotency in Human Dermal Fibroblasts Treated with Xenopus Laevis Egg Extract Fractions". Digital WPI, 2014. https://digitalcommons.wpi.edu/etd-dissertations/207.
Pełny tekst źródłaPhilip, Diana Liz. "The Influence of Synthetic Microenvironments in Determining Stem Cell Fate". University of Akron / OhioLINK, 2021. http://rave.ohiolink.edu/etdc/view?acc_num=akron1627669247178055.
Pełny tekst źródłaLoison-Robert, Ludwig. "Cellule souche gingivale : origine et multipotence". Thesis, Paris Est, 2016. http://www.theses.fr/2016PESC0083/document.
Pełny tekst źródłaGingiva is a natural regeneration model thanks to its "ad integrum" healing capability. Gingival fibroblasts are the main actors of this property. These cells, the main cellular component of the gingival connective tissue, regulate the inflammatory responses and healing process. This tissue contains, like many others, mesenchymal stem cells; which also partly explain these regenerative abilities. Moreover, as the gingiva is abundant and easily accessible, the use of these stem cells may interest cell therapy or in vitro model tissues responses. In this work, we demonstrated that Stem Cells Derived from Human Gingiva (SCHG) have common properties with neural crest adult stem cells. These cells can be called "stem cells" for their ability to self-renew, adhere to plastic and to differentiate. First, we have shown that the method and the culture products used for isolation of gingival fibroblasts from gingival biopsy had an influence on the obtained cells. Secondly, an analysis of in vitro clonal populations of gingival fibroblasts has shown that gingival fibroblasts are composed of subpopulations that express specific markers of stem cells and neural crests. In addition to their embryological origin, the study of their multipotency was also characterized after expansion and depending on the used additives. Finally, two examples of using these cells and dental pulp stem cells as a model to study the in vitro biocompatibility of biomaterials have been developed, mimicking oral mucosa or dentin reactions (reparative or reactional)
Bondue, Antoine. "Mesp1 functions in multipotent cardiovascular progenitor specification". Doctoral thesis, Universite Libre de Bruxelles, 2009. http://hdl.handle.net/2013/ULB-DIPOT:oai:dipot.ulb.ac.be:2013/210319.
Pełny tekst źródłaDoctorat en sciences médicales
info:eu-repo/semantics/nonPublished
McBride, Sarah. "ELUCIDATING BIOPHYSICAL CUES CONDUCIVE TO TARGETED MULTIPOTENT CELL DIFFERENTIATION". Case Western Reserve University School of Graduate Studies / OhioLINK, 2008. http://rave.ohiolink.edu/etdc/view?acc_num=case1196435881.
Pełny tekst źródłaClaudinot, Stéphanie. "Potentialité des keratinocytes clonogéniques : filiation avec les cellules souches". Paris 6, 2004. http://www.theses.fr/2004PA066058.
Pełny tekst źródłaHeins, Nico. "Intrinsic fate determinants of neural and multipotent CNS precursor cells". Diss., lmu, 2005. http://nbn-resolving.de/urn:nbn:de:bvb:19-45202.
Pełny tekst źródłaCraig, Laura A. "Differentiation of multipotent skin derived precursor cells into skeletal muscle". Thesis, McGill University, 2004. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=80245.
Pełny tekst źródłaZepponi, Vanessa. "Les progéniteurs multipotents exprimant Flt3 source des précurseurs T extra-thymiques". Phd thesis, Université René Descartes - Paris V, 2012. http://tel.archives-ouvertes.fr/tel-00806094.
Pełny tekst źródłaAli, Hamad. "Neuronal potential of umbilical cord blood non-hematopoietic multipotent stem cells". Thesis, University of Newcastle Upon Tyne, 2011. http://hdl.handle.net/10443/1277.
Pełny tekst źródłaDowney, Jennifer. "Identification and isolation of multipotent stromal cells from human skeletal muscle". Mémoire, Université de Sherbrooke, 2013. http://hdl.handle.net/11143/6296.
Pełny tekst źródłaKaanta, Alice. "Evidence for a Novel Multipotent Mammary Progenitor with Pregnancy-Specific Activity". Thesis, Harvard University, 2012. http://dissertations.umi.com/gsas.harvard:10069.
Pełny tekst źródłaNakano, Ichiro. "Maternal embryonic leucine zipper kinase (MELK) regulates multipotent neural progenitor proliferation". Kyoto University, 2008. http://hdl.handle.net/2433/135920.
Pełny tekst źródłaYoshikawa, Katsuhiro. "Multipotent stem cells are effectively collected from adult human cheek skin". Kyoto University, 2013. http://hdl.handle.net/2433/174817.
Pełny tekst źródłaMitutsova, Violeta. "Cellules souches du muscle squelettique : étude d'une population capable de différenciation multipotente". Thesis, Montpellier, 2015. http://www.theses.fr/2015MONTT025/document.
Pełny tekst źródłaThe use of stem cells is a promising approach for the treatment of neuromuscular degenerative diseases. Many studies currently focus on embryonic stem cells (ES) and induced pluripotent stem cells (IPs) for use in regenerative medicine. But some problems remain for their use in cell therapy in particular the potential of these cells to form teratomas. This problem requires both ES and IPs to be differentiated towards a specific cell type. Such induction of differentiation can lead to additional risks such as genetic drift or various sources of contamination.The adult skeletal muscle, has a high plasticity and regenerative capacity, it contains a stem cell population that is specific for muscle, and has been isolated and studied in the laboratory. Adult skeletal Muscle-Derived Stem Cells, MDSC repopulate and repair damaged skeletal muscle with high efficiency in a few days, even in the presence of endogenous satellite cells. (Arsic et al Exp. Cell Res. 2008). The host laboratory is characterizing this cell population and its histological identity and testing the tissue repair potential of transplanted MDSC in mouse models, as well as their bio-distribution for therapeutic use.My thesis work addressed the study of this stem cells population isolated from skeletal muscle showing low adhesion to substrate. Poor/low adherence is an interesting property because in addition to be defined as closer to the pluripotent state, this property is associated with a higher migration capability. This population of muscle stem cells should be easier to use than pre-differentiated stem cells in regenerative medicine. In this perspective it is interesting to use multipotent stem cells that are close to pluripotent cells in terms of differentiation and regenerative capacity, but without the inconveniencies like teratogenic risk and uncontrolled proliferation, as well as expensive and time-consuming cell culture.At the beginning of my thesis I was interested by the different populations of cells present in muscle and I focused my work on known markers of stem cells, whose presence has been established in skeletal muscle, but not clearly identified histologically. Muscle stem cells expressed the pluripotency factor Sox2, but also markers, such as BCRP1/ABCG2, Sca-1 and SSEA1. I have examined the potential of MDSC to differentiate in vitro into several cell types such as cardiac pacemaker-like cells, insulin-producing cells and cells that exhibit neuronal markers. I also focused on the possible therapeutic applications of MDSC, particularly in the case of heart rhythm problems and in the case of insulin-dependent diabetes. For these in vivo studies of the repair potential of MDSC, a single systemic injection is carried out in mouse models of the diseases. The histological recovery of injected MDSC into target organs also raises the question of the biodistribution of MDSC in the body. Therefore I spent more than a year of my doctoral thesis to address this issue and showed a targeted recruitment of MDSC to injured tissue or organs within 48h of their systemic injection
Hjerling-Leffler, Jens. "Sensory neurons: stem cells and development /". Stockholm, 2006. http://diss.kib.ki.se/2006/91-7140-667-0/.
Pełny tekst źródłaLi, Yueying. "Vieillissement des cellules stromales mésenchymateuses de la moelle osseuse : implications en médecine régénérative". Thesis, Université de Lorraine, 2015. http://www.theses.fr/2015LORR0099/document.
Pełny tekst źródłaToday with their properties of differentiation into specific cells types, mesenchymal stromal cells (MSC) can be used in regenerative medicine. Bone marrow (BM) is the better characterized one. The researchers have proven that with increasing passage number in culture the proliferation and differentiation potential of MSC decrease. In parallel many researchers have showed the impact of donor age on MSC properties remains controversial. The aim of our study was to better understand the effect of donor age but also culture passages on the proliferation and differentiation ability of bone marrow mesenchymal stromal cells. The samples were separated into 4 groups depending on the donor age (<20 years; 20-40 years; 40-60 years; > 60 years) and The samples were cultured for 5 passages. The results obtained show that the MSC proliferative capacity obtained from young donors is greater than that of cells from older donors. In addition, the proliferative capacity decreases with increasing passage number in culture. In parallel, the ability of colony-forming unit-fibroblast, measured by the CFU-F assay, decreases slightly depending on the age of the donors but significantly depending on the passage. Finally, the MSC differentiation ability decreases according to the passage of the cells but also depending on the donor age. Our study shows that the properties of bone marrow derived MSC are modified not only during amplification in vitro but also in terms of donor age
Ferré, François. "Isolation et caractérisation des cellules souches gingivales : étude de leur potentiel multipotent". Phd thesis, Université René Descartes - Paris V, 2013. http://tel.archives-ouvertes.fr/tel-01017172.
Pełny tekst źródłaAhmed, Muhammad A. "Exploring the impact of hypoxia mimetic agents on multipotent stem cell biology". Thesis, Keele University, 2018. http://eprints.keele.ac.uk/4532/.
Pełny tekst źródłade, Almeida Rocha Ponzoni Raquel. "Raman spectroscopy studies of multipotent stromal cells differentiation for bone engineering application". Thesis, University of York, 2017. http://etheses.whiterose.ac.uk/18435/.
Pełny tekst źródłaBown, Andre B. J. "The Utilization of Multipotent Mesenchymal Stromal Cell Transplantation to Improve Fascia Repair". Youngstown State University / OhioLINK, 2013. http://rave.ohiolink.edu/etdc/view?acc_num=ysu1376390936.
Pełny tekst źródłaRondeau, Vincent. "Rôle de la désensibilisation de CXCR4 dans la spécification lympho-myéloïde des progéniteurs hématopoïétiques multipotents. Lymphoid differentiation of hematopoietic stem cells requires efficient Cxcr4 desensitization New method to obtain lymphoid progenitors CXCR4-driven mitochondrial metabolic pathways shape the lympho-myeloid fate of hematopoietic multipotent progenitors". Thesis, université Paris-Saclay, 2020. http://www.theses.fr/2020UPASQ022.
Pełny tekst źródłaHematopoietic stem and progenitor cells (HSPCs), including the multipotent progenitors (MPPs), are responsible for replenishing immune cells. They reside in bone marrow (BM) endosteal and (peri)-vascular niches, which provide all cellular and molecular components required for their lifelong maintenance and fate. Among them, the CXCL12 chemokine and one of its receptor, CXCR4, exert a dominant role in promoting HSPC retention and quiescence. These processes are deregulated in the WHIM Syndrome (WS), a rare immunodeficiency caused by inherited heterozygous autosomal gain-of-function CXCR4 mutations that affect homologous desensitization of the receptor. Clinically, WS is notably characterized by severe, chronic circulating lymphopenia whose mechanisms remain to be elucidated. Using a mouse model carrying a naturally occurring WS-linked Cxcr4 mutation as well as human BM and blood samples, we explored the possibility that the lymphopenia in WS originates from defects at the HSPC level in BM. We reported that Cxcr4 desensitization is required for lymphoid differentiation of HSPCs and further identified the MPP stage as defective in mutant mice. The divergence between lymphoid and myeloid lineages occurs at the MPP stage, which is composed of distinct subpopulations, i.e., MPP2 and MPP3 are reported as distinct myeloid-biased MPP subsets that operate together with lymphoid-primed MPP4 to control blood leukocyte production. Our understanding of how cell-extrinsic niche-related and cell-intrinsic cues drive the lymphoid versus myeloid fate decision of MPPs is still fragmentary. Therefore, my PhD project aimed at determining whether and how CXCR4 signaling regulates bioenergetics demands of MPPs and at understanding how these metabolic pathways shape the lympho-myeloid fate of MPPs. We unraveled a myeloid skewing of the HSPC compartment in BM of WS mice and patients. In mutant mice, this partly relied on the contraction of the MPP4 pool and on cell-autonomous molecular and metabolic changes that reprogramed MPP4 away from lymphoid differentiation. Interestingly, chronic treatment with the CXCR4 antagonist AMD3100 normalized mitochondrial metabolism and fate of MPP4, while correcting circulating lymphopenia in WS mice. This study provides evidence that CXCR4 signaling acts as an essential gatekeeper for integrity of the mitochondrial machinery, which in turn controls lymphoid potential of MPP4
Eydt, Carina [Verfasser]. "Equine multipotente, mesenchymale Stromazellen (MSCs): Optimierung der Gewinnung, Expansion und Kultivierung / Carina Eydt". Hannover : Bibliothek der Tierärztlichen Hochschule Hannover, 2016. http://d-nb.info/1124565647/34.
Pełny tekst źródłaMayeuf, Alicia. "Choix du destin cellulaire des progéniteurs multipotents du somite, chez l'embryon de souris". Paris 6, 2013. http://www.theses.fr/2013PA066495.
Pełny tekst źródłaThe dorsal part of the somite, the dermomyotome contains multipotent Pax3+ progenitors, which give rise to different cell types such as skeletal muscle, dermal, endothelial, mural and brown adipose cells. The aim of this thesis was to understand mechanisms underlying cell fate decisions in this context in the mouse embryo. We have first shown that the Notch signaling pathway directs multipotent progenitors towards a vascular instead of a myogenic fate, by acting on the Pax3 :Foxc2 genetic equilibrium. To determine if Foxc1, the homologue of Foxc2, is also implicated in this mecanism, we have conditionally deleted both genes in Pax3+ progenitors. We document new phenotypes, including a reduction in vascular, cells, notably endothelial cells in the forelimb, where, surprisingly myogenic cells are also absent, leading to a number of possible hypotheses. Foxc2 is also implicated in the differentiation of brown adipose tissue, which we show is a derivative of Pax3+ cells in the dermomyotome. We have studied the development of this tissue in the embryo and propose a model in two steps, with initial formation of an “undifferentiated adipogenic mass” which subsequently differentiates into brown adipocytes. Gain and loss of function approaches suggest that Foxc1/2 play a role in the control of mitochodrial function during the differentiation of brown adipocytes in the embryo. This role may also be played by Foxc1 in the slow fibers of skeletal muscle where it is specifically expressed in the adult
Gadepalli, Venkat Sundar. "ISOLATION AND CHARACTERIZATION OF MULTIPOTENT LUNG STEM CELLS FROM p53 MUTANT MICE MODELS". VCU Scholars Compass, 2014. http://scholarscompass.vcu.edu/etd/3644.
Pełny tekst źródłaTraveset, Martínez Laia 1992. "New insights into transcription that preserve hematopoietic stem cell homeostasis". Doctoral thesis, Universitat Pompeu Fabra, 2020. http://hdl.handle.net/10803/670105.
Pełny tekst źródłaEl manteniment de l'hematopoesi en condicions basals i en situacions d’estrès depèn de l'aptitud de les cèl·lules mare i progenitores hematopoètiques (HSPCs) de la medul·la òssia. Les cèl·lules mare hematopoètiques (HSC) són capaces d’adaptar-se a l’estrès mitjançant l'ampliació dels seus nombres i l'augment de la producció de cèl·lules sanguínies. Aquest procés, dinàmic i molt complex, ha d'estar totalment regulat per tal de mantenir un equilibri entre la diferenciació de les HSCs i la necessitat de mantenir un nombre constant de HSCs. No obstant això, la maquinària molecular encarregada d'aquesta regulació no ha estat encara completament caracteritzada. Les HSCs tan sols representen una petita proporció dins del compartiment de HSPCs el qual també inclou la progènie immediata de les HSCs, els progenitors multipotents (MPP). Els MPPs són una població cel·lular que conserva el potencial de llinatge complet, però que presenta una capacitat d'autorenovació limitada en comparació amb les HSCs. En aquesta tesi explorem un nou mecanisme important pel manteniment i la protecció de la funció de les HSCs sota estrès. Hem caracteritzat com la cèl·lula mare hematopoètica funciona després d’un trasplantament en sèrie, després d’una lesió de mielosupressió i després d’un protocol d’irradiació total. Per tant, presentem resultats in vivo que diluciden un nou mecanisme transcripcional implicat en el manteniment de la viabilitat i la capacitat d’autorenovació de les HSC i que restringeix la seva diferenciació cap a MPPs, en situacions en què el sistema hematopoètic ha de mantenir constant el dipòsit de cèl·lules mare per tal d’evitar el seu esgotament.
Viana, Gaza Maria Elisabet. "Disseny i síntesi de nous compostos multipotents per al tractament de la malaltia d’Alzheimer". Doctoral thesis, Universitat de Barcelona, 2013. http://hdl.handle.net/10803/110463.
Pełny tekst źródłaThe main factor triggering the process of neurodegeneration characteristic of Alzheimer's disease (AD) is the so-called β-amyloid peptide (Aβ). The increased formation of this peptide and its aggregation are placed at the beginning of a cascade of neurotoxic processes culminating in widespread neuronal dysfunction and death. Currently, there are several "disease-modifying" anti-Alzheimer drug candidates in clinical trials aimed at inhibiting the formation or aggregation of Aβ. It is expected that these compounds can prevent or slow the neurodegeneration of AD. The current treatment available for the treatment of AD is still dominated by a group of drugs that were developed to treat its symptoms, which appear as a result of the decrease in the production of various neurotransmitters, particularly acetylcholine (ACh) at the central nervous system. Consequently, the use of cholinomimetic agents capable to compensate this deficit should reduce cholinergic symptoms of AD. In fact, four out of the five drugs currently marketed for the treatment of AD are a group of inhibitors of the enzyme acetylcholinesterase (AChE), the enzyme responsible for the hydrolysis of ACh at the synapses, which increase levels of the neurotransmitter, thus reducing the symptoms of AD. Recently, it has been demonstrated that the enzyme AChE can bind to Aβ accelerating its aggregation, increasing the neurotoxicity and promoting cognitive decline. The binding site of AChE to Aβ is the so-called peripheral site, which is located at the entrance of a catalytic gorge of about 20 Å length, at the bottom of which the catalytic site of the enzyme is placed, where the hydrolysis of ACh takes place. Thus, the blockade of the peripheral site of AChE should lead to an inhibition of Aβ aggregation and of the neurodegenerative cascade. In addition, the particular architecture of the enzyme where the active and peripheral sites are separated but close enough to allow a molecule to simultaneously interact with the two binding sites has led to the development of dual binding site AChE inhibitors, which are very promising anti-Alzheimer drug candidates expected to positively alter the course of disease. Moreover, due to the multifactorial nature of the etiology of AD, development of multipotent compounds capable of interacting with different biological targets involved in the disease is beginning to be considered as the only way to achieve efficient impact on the course of the disease. Aβ aggregation plays a crucial role in the pathogenesis of AD, inducing processes in the neurodegenerative cascade and oxidative stress due to overproduction of reactive oxygen species (ROS). It also appears that the Cu2+ and Fe3+ ions are capable of generating ROS, after binding to the Aβ For these reasons, the metal ions and oxidative stress are interesting targets to modulate by multipotent anti-Alzheimer drug candidates. Thus, it has been shown that the compounds capable of chelating metal ions are able to inhibit the production of ROS and the use of natural antioxidants is also being considered to block the oxidative stress involved in Aβ. The aim of the present PhD is to develop multipotent compounds capable to interact with different biological targets involved in AD, namely cholinesterases, Aβ and oxidative stress, in order to block its neurodegenerative cascade.
Grau, Vorster Marta. "Development and characterisation of advanced cell therapies based on multipotent mesenchymal stromal cells and virus-specific Tlymphocytes". Doctoral thesis, Universitat Autònoma de Barcelona, 2019. http://hdl.handle.net/10803/669379.
Pełny tekst źródłaInnovative therapies are being developed worldwide to tackle unmet clinical needs. In particular, progress in advanced therapy medicinal products (ATMP) has shown great promise for the treatment of diseases with no other option available. However, researchers and regulatory authorities deal with the sophisticated nature of these medicines, and struggle to standardise both production protocols and final product formulation. Challenges related to the living nature of these products include high donor intervariability and complex mechanisms of action, which are sometimes not completely understood. Additionally, these newly therapies need to demonstrate biological activity with potency assays. This dissertation comprises the development and characterisation of two different ATMP based on multipotent mesenchymal stromal cells (MSC) and virus-specific T cells (VST). On the one hand, assessment of identity and potency for product release of MSC isolated from Wharton’s jelly (WJ) and bone marrow (BM) in the context of current good manufacturing practice (cGMP) production is performed. In this regard, we aimed at proposing: a) a potency assay for assessing immunomodulation capacity of MSC; b) the revision of HLA-DR expression profile for MSC definition criteria; and c) the application of risk management methodologies in the assessment of product quality. The optimisation of an immunopotency assay, validated, and approved by the competent authority for product release is presented. Moreover, other quality attributes of MSC are addressed. Regarding BM-MSC, the apparently random expression of HLA-DR, a marker that was expected negative in expansion cultures of MSC, is studied in clinical grade productions. Our findings showed correlation of HLA-DR expression with levels of IL-17F and IL-33. Expression of HLA-DR did not affect MSC identity, differentiation potential nor immunomodulatory capacity. To further strengthen these outcomes, interlaboratory studies were performed obtaining similar results. Furthermore, the use of either human sera or platelet lysate supplements showed no differences in terms of HLA-DR expression. A risk management assessment methodology was also implemented as a tool for quality by design to detect weaknesses of an established bioprocess involving MSC products already in clinical trials. On the other hand, regarding T lymphocytes, the development of a protocol for ex vivo expansion of VST was performed. VST therapy is intended for immunocompromised patients, which are susceptible of reactivation or de novo infection of herpesviruses among others. This is the case of cytomegalovirus (CMV) that undergoes a mild infection in healthy individuals but has been associated to a high morbidity and mortality in immunocompromised individuals. Unfortunately, available antiviral drugs can produce toxic side effects and are not always effective. Adoptive immunotherapy offers an alternative approach for those patients in a critical situation with no other therapeutic option. Therefore, we developed a protocol for VST scale-up manufacture easily transferable to pharmaceutical standards. Following with the method proposed, we obtained large number of CMV pp65-specific T cells after 14-day co-culture with pp65 pulsed dendritic cells. Culture was based on G-Rex bioreactor technology and supplemented with IL-2, IL-7, IL-15, anti-CD3 and anti-CD28 antibodies. The final product was extensively characterised in terms of identity, purity and potency. VST product was comprised of both CD4+ and CD8+ T lymphocytes, and effector memory T cells represented the major subset, which are known to provide effector function. Most importantly, we successfully demonstrated pp65 specific cytotoxicity of the expanded cells. Interestingly, complete HLA mismatch alloreactivity resulted in less than 5% cell lysis. In summary, a feasible protocol transferable to cGMP was described for an in vitro safe and effective product, which remain functional after thawing, thus providing practical evidence for the generation of an allogeneic third-party bank. Future perspectives would include the manufacture of multivirus-specific T cells.
Marshall, Gregory Paul. "Neurospheres and multipotent astrocytic stem cells neural progenitor cells rather than neural stem cells /". [Gainesville, Fla.] : University of Florida, 2005. http://purl.fcla.edu/fcla/etd/UFE0010047.
Pełny tekst źródłaTypescript. Title from title page of source document. Document formatted into pages; contains 97 pages. Includes Vita. Includes bibliographical references.
Tripathi, Pratibha. "Isolation of multipotent astroglia form the adult stem cell niche and the injured brain". Diss., lmu, 2009. http://nbn-resolving.de/urn:nbn:de:bvb:19-104224.
Pełny tekst źródłaMacDonald, Stephen Christopher. "Oligodendrocytes and motoneurons, two cholinergic cell types derived from multipotent spinal neuroepithelial precursor cells". Thesis, National Library of Canada = Bibliothèque nationale du Canada, 2000. http://www.collectionscanada.ca/obj/s4/f2/dsk1/tape2/PQDD_0016/NQ53066.pdf.
Pełny tekst źródłaMaestri, Valentina <1984>. "Design and synthesis of multipotent drugs: application to Alzheimer's disease and benign prostatic hyperplasia". Doctoral thesis, Alma Mater Studiorum - Università di Bologna, 2013. http://amsdottorato.unibo.it/5280/.
Pełny tekst źródłaLi, Zhengdong [Verfasser]. "Influence of surface topographic microstructure on behaviors of multipotent and pluripotent stem cells / Zhengdong Li". Berlin : Freie Universität Berlin, 2018. http://d-nb.info/1171431236/34.
Pełny tekst źródłaDePaul, Marc A. "INTRAVENOUS MULTIPOTENT ADULT PROGENITOR CELL TREATMENT DECREASES INFLAMMATION LEADING TO FUNCTIONAL RECOVERY FOLLOWINGSPINAL CORD INJURY". Case Western Reserve University School of Graduate Studies / OhioLINK, 2015. http://rave.ohiolink.edu/etdc/view?acc_num=case1451317702.
Pełny tekst źródłaTanigaki, Kenji. "Notch1 and Notch3 instructively restrict bFGF-responsive multipotent neural progenitor cells to an astroglial fate". Kyoto University, 2001. http://hdl.handle.net/2433/150564.
Pełny tekst źródłaMajo, François. "Renouvellement de l'épithélium de la cornée chez les mammifères". Paris 7, 2005. http://www.theses.fr/2005PA077184.
Pełny tekst źródłaRuggiero, Antonella. "Impact of Wnt signalling on multipotent stem cell dynamics during Clytia hemisphaerica embryonic and larval development". Thesis, Paris 6, 2015. http://www.theses.fr/2015PA066561/document.
Pełny tekst źródłaThe aim of this work was to extend our understanding of the mechanisms regulating stem cell formation, specification and differentiation by studies in the non-bilaterian metazoan model Clytia hemisphaerica. Clytia, like other hydrozoan cnidarians, possess a particular population of multipotent stem cells called interstitial cells (i-cells), present during larval development and in the adult medusa, which are able to give rise both to somatic cell types and to gametes.In bilaterian animals Wnt/β-catenin signalling regulates fundamental developmental processes such primary body axis specification, but also regulates stem cell proliferation, lineage specification and differentiation. I investigated the role of Wnt/β-catenin signalling in i-cell specification and differentiation. The results obtained suggest that Wnt/β-catenin signalling is involved in the last step of differentiation for certain neuronal cell types, but not for somatic cell fate choice. In the second part of my study I investigated the role of Wnt/β-catenin signalling in i-cell formation during embryogenesis. The results indicated that during normal development i-cell formation is Wnt/β-catenin independent and probably driven by inheritance of germ plasm containing localised mRNAs from the egg animal pole. In contrast in embryo re-patterning following embryo bisection, Wnt/β-catenin signalling appears to be necessary for de novo i-cell formation in the absence of germ plasm. Thus two distinct mechanisms can lead to i-cell formation during embryogenesis. Overall the results obtained provided a better picture of how i-cells and their derivatives arise during embryogenesis and larval development
Koyama, Noriaki. "Investigation of chondrogenic differentiation capability using human orofacial tissue-derived multipotent cells and human iPS cells". Kyoto University, 2013. http://hdl.handle.net/2433/174775.
Pełny tekst źródłaSCHISCHMANOV, NICOLA. "New Hypothesis on the Origin of Metastases". Nagoya University School of Medicine, 2013. http://hdl.handle.net/2237/17613.
Pełny tekst źródłaTan, Yuan. "Using induced pluripotent stem cells to establish disease models with neurodegenerative disorders". Thesis, University of Macau, 2018. http://umaclib3.umac.mo/record=b3952490.
Pełny tekst źródłaAngstmann, Michael [Verfasser], i Stefan [Akademischer Betreuer] Wölfl. "Characterization of cell-matrix interactions during multipotent mesenchymal stromal cell (MSC) differentiation / Michael Angstmann ; Betreuer: Stefan Wölfl". Heidelberg : Universitätsbibliothek Heidelberg, 2011. http://d-nb.info/1179230140/34.
Pełny tekst źródłaEdri, Shlomit. "Date with destiny : genetic and epigenetic factors in cell fate decisions in populations of multipotent stem cells". Thesis, University of Cambridge, 2019. https://www.repository.cam.ac.uk/handle/1810/288380.
Pełny tekst źródłaWu, Shan Ph D. Massachusetts Institute of Technology. "Rational design to control multipotent stromal cell migration for applications in bone tissue engineering and injury repair". Thesis, Massachusetts Institute of Technology, 2011. http://hdl.handle.net/1721.1/67198.
Pełny tekst źródłaCataloged from PDF version of thesis.
Includes bibliographical references (p. 151-162).
Multipotent stromal cells derived from bone marrow hold great potential for tissue engineering applications because of their ability to home to injury sites and to differentiate along mesodermal lineages to become osteocytes, chondrocytes, and adipocytes to aid in tissue repair and regeneration. One key challenge, however, is the scarcity of MSC numbers isolated from in vivo, suggesting a role for biomimetic scaffolds in the cells' ex vivo expansion before reintegration into target tissue. Toward this end, immobilized epidermal growth factor (tEGF) has recently been found to promote MSC survival and proliferation and is a prime candidate to be incorporated into scaffolds to control MSC behavior. To rationally and effectively design scaffolds to drive MSC responses of survival, proliferation, migration, and differentiation, we must first understand these responses and the underlying protein signaling pathways that mediate them. While our knowledge of MSC behavior is limited as a field, MSC migration is particularly less studied despite being critical for tissue and scaffold infiltration. In this thesis, we quantitatively investigate the effects of tEGF and extracellular matrix (ECM) on MSC migration response and signaling. We take a systems level computational view to show a combined biomaterials and small molecule approach to control MSC migration. Cell migration is a delicately integrated biophysical process involving polarization and protrusions at the cell front, adhesion and translocation of the cell body through contractile forces, followed by disassembly of adhesion complexes at the cell rear to allow detachment and productive motility. This process is mediated by a multitude of crosstalking signaling pathways downstream of integrin and growth factor activation. Using a poly(methyl methacrylate)-grafted-poly(ethylene oxide) (PMMA-g-PEO) copolymer base, we modify the PEO sidechains with immobilized epidermal growth factor (tEGF) as a model system for biomimetic scaffolds. We systematically adsorb fibronectin, vitronectin, and collagen ECM proteins to alter surface adhesiveness and measure MSC migration responses of speed and directional persistence alongside intracellular activities of EGFR, ERK, Akt, and FAK phosphoproteins. While tEGF and ECM proteins differentially affected signaling and migration, univariate correlations between signals and responses were not informative, prompting the need for multivariate modeling to identify key patterns. Using decision tree "signal-response" modeling, we predicted that inhibiting ERK on collagen-adsorbed tEGF polymer surfaces would increase cell mean free path (MFP) by increasing directional persistence. We confirmed this experimentally, successfully demonstrating a two-layer approach-"coarse" biomaterials followed by small molecules "fine-tuning"-to precisely and differentially control MSC migration speed and persistence, setting the stage for combination therapies for bone tissue engineering.
by Shan Wu.
Ph.D.