Gotowe bibliografie tematyczne / Multi-photon excitation microscopy

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Gotowa bibliografia na temat „Multi-photon excitation microscopy”

Autor: Grafiati

Data publikacji: 6 września 2023

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Artykuły w czasopismach na temat "Multi-photon excitation microscopy"

Piston, David W. "Multi-Photon Excitation Microscopy: An Old Idea in Quantum Theory Applied to Modern Scientific Problems." Microscopy and Microanalysis 6, S2 (2000): 1180–81. http://dx.doi.org/10.1017/s1431927600038393.

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Multi-photon excitation microscopy provides attractive advantages over confocal microscopy for three-dimensionalry resolved fluorescence imaging and photochemistry. The most commonly used type of multi-photon excitation is two-photon excitation where simultaneous absorption of two photons leads to a single quantitized event. The powerful advantages of using two-photon excitation microscopy arise from the basic physical principle that the absorption depends on the square of the excitation intensity. In practice, two-photon excitation is generated by focusing a single pulsed laser through the microscope. As the laser beam is focused, the photons become more crowded, but the only place at which they are crowded enough to generate an appreciable amount of two-photon excitation is at the focus. Above and below the focus, the photon density is not high enough for two of them to interact with a single fluorophore at the same time. This dramatic difference between confocal and two-photon excitation microscopy is shown in Fig. 1.

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ROTHSTEIN, EMILY C., MICHAEL NAUMAN, SCOTT CHESNICK, and ROBERT S. BALABAN. "Multi-photon excitation microscopy in intact animals." Journal of Microscopy 222, no. 1 (2006): 58–64. http://dx.doi.org/10.1111/j.1365-2818.2006.01570.x.

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Masters, Barry R., and Peter T. C. So. "Multi-photon Excitation Microscopy and Confocal Microscopy Imaging of In Vivo Human Skin: A Comparison." Microscopy and Microanalysis 5, no. 4 (1999): 282–89. http://dx.doi.org/10.1017/s1431927699990311.

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Abstract: We compare here multi-photon excitation microscopy and tandem scanning reflected light confocal microscopy for the microscopic observation of human skin in vivo. Multi-photon excitation is induced by a 80-MHz pulse train of femtosecond laser pulses at 780 nm wavelength. This nonlinear microscopic technique is inherently suitable for tissue fluorescence imaging because of its deeper penetration depth and lower specimen photodamage. This technique has noninvasively obtained tissue structural information in human epidermis and dermis. Alternatively, tandem scanning confocal light microscopy based on a white light source can provide video-rate image acquisition with high resolution and high contrast. Reflected light confocal methods have been used to obtain images from the skin surface to the epidermal–dermal junction. The relative merits of these two techniques can be identified by comparing three-dimensionally resolved images obtained from the forearm skin of the same volunteer.

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Cheng, Ping-chin, Chi-Kuang Sun, Fu-Jen Kao, and Bai-Ling Lin. "Non-linear Spectral Microscopy-Multi-Photon Fl, SHG and THG." Microscopy and Microanalysis 7, S2 (2001): 1026–27. http://dx.doi.org/10.1017/s1431927600031202.

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The non-linear nature of multi-photon fluorescence (FL) excitation, SHG and THG restricts the signal detecting volume to the vicinity of the focal point. As a result, the technology has intrinsic optical sectioning capability. The use of multi-photon fluorescence excitation also allows micro-fluorometry at high spatial resolution. Figure 1 shows a conventional optical micrograph of maize protoplasts, the time lapse fluorescence spectral change from a single chloroplast is shown in FIG 2. Under high intensity illumination, biological specimen not only emits fluorescence, but also generates harmonic emissions. in addition to the Ti-sapphire laser commonly used in multiphoton microscopy, the use of ultra-fast Cr-fosterite laser made simultaneous detecting two- and three-photon fluorescence, SHG and THG possible. in addition to the fluorescence signals generated by multi-photon excitation process, non-linear phenomena such as harmonic generation can also provide useful information about the structure and optical properties of a specimen (Kao et al., 2000). Simultaneous recording the spectral response in an image (x-y-λ) can provide insight about the nature of the signal.

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Kao, F. J., B. L. Lin, and P. C. Cheng. "Multi-photon Fluorescence Micro-spectroscopy of Plant Tissues." Microscopy and Microanalysis 6, S2 (2000): 808–9. http://dx.doi.org/10.1017/s1431927600036539.

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Considering its non-linear nature, two-photon excitation may generate very different spectral response in samples when compared with single photon excitation. It is thus necessary to measure the two-photon spectra of samples, so that the two-photon fluorescence microscopic images can be properly interpreted. Fluorescence spectra obtained from bulk samples may not provide useful information for microscopy. For instance, due to the relatively small contribution to the total fluorescence intensity, a small number of fluorescent particles in a generally fluorescing specimen may escape detection when the spectrum of the specimen as a whole is obtained. Under two-photon excitation, the background noise can be greatly reduced due to the naturally limited excitation volume of focused laser beam. In addition, signals resulted from second harmonic generation (SHG) may be mixed with low level broad-band background autofluorescence which is commonly found in biological specimen. Therefore, measuring fluorescence spectrum from a micro-focused volume is essential for the proper interpretation of multi-photon fluorescence images.

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Guo, Yong, Hongyi Han, Luwei Wang, et al. "Label free deep penetration single photon microscopic imaging with ultralong anti-diffracting beam." Applied Physics Letters 121, no. 2 (2022): 023701. http://dx.doi.org/10.1063/5.0097959.

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Label free single photon microscopic imaging has natural advantages in noninvasive in vivo tissue imaging such as high resolution and rapid imaging speed. Although label free multi-photon microscopy can be used for imaging thick tissue samples, it requires high excitation light power and is phototoxic to the samples. Conventional label free single photon microscopy requires lower excitation light power, but it has limited imaging depth. Observing some highly scattering thick tissue samples with single photon microscopy is a great challenge. To solve the problem, we developed a label free deep penetration single photon microscopic imaging technique with an ultralong anti-diffracting (UAD) beam. The penetrating ability of the UAD beam was verified by passing through turbid media and performed with autofluorescence of chloroplasts in fresh Epipremnum aureum leaves. Benefiting from the anti-diffracting properties and the elongated focal depth of the UAD beam, single photon UAD microscopy has deeper penetration depth and better anti-scattering ability and is one of the ideal methods to observe the deep structure of biological samples.

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Cheng, P. C., B. L. Lin, F. J. Kao, C. K. Sun, and I. Johnson. "Multi-Photon Fluorescence Spectroum of Common Nucleic Acid Probes." Microscopy and Microanalysis 6, S2 (2000): 820–21. http://dx.doi.org/10.1017/s143192760003659x.

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Fluorescent probes are commonly used in biological fluorescence microscopy for tracking specific structures and sub-cellular compartments, and for indicating cellular ionic conditions. Recent development in multi-photon fluorescence microscopy has greatly expanded the usage of fluorescent probes in biomedical research. Considering its non-linear nature, two-photon excitation may generate very different fluorescence spectral response in the sample when compared with single photon excitation. It is thus necessary to measure the two-photon spectra of various fluorescent probes, so that two-photon fluorescence microscopy may be operated effectively and the images properly interpreted. This report represents the first installment of a continued effort in characterizing the multi-photon fluorescence spectra of commonly used bio-probes.Two-photon fluorescence spectra excited with near infrared at 780nm were obtained with a SpectraPro-500 spectrophotometer (Acton Research) equipped with a TE-cooled PMT and coupled to a Spectra-Physics Tsunami Ti-sapphire laser pumped by a Coherent Verdi solid-state laser operated at 85MHz, l00fs pulse.

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Mertz, J. "Molecular photodynamics involved in multi-photon excitation fluorescence microscopy." European Physical Journal D - Atomic, Molecular and Optical Physics 3, no. 1 (1998): 53–66. http://dx.doi.org/10.1007/s100530050148.

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Schweitzer, Andreas, Heinz Eipel, and Christoph Cremer. "Rapid image acquisition in multi-photon excitation fluorescence microscopy." Optik 115, no. 3 (2004): 115–20. http://dx.doi.org/10.1078/0030-4026-00339.

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Goswami, Debabrata, Dhiman Das, and Soumendra Nath Bandyopadhyay. "Resolution enhancement through microscopic spatiotemporal control." Faraday Discussions 177 (2015): 203–12. http://dx.doi.org/10.1039/c4fd00177j.

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Operating at biologically benign conditions, multi-photon fluorescence imaging microscopy has benefitted immensely from recent developments in microscopic resolution enhancement. Fluorescence microscopy continues to be the best choice for experiments on live specimens, however, multi-photon fluorescence imaging often suffers from overlapping fluorescence of typical dyes used in microscopy, limiting its scope. This limitation has been the focus of our research where we show that by making simple modifications to the laser pulse structure, it is possible to resolve these overlapping fluorescence complications. Specifically, by using pairs of femtosecond pulses with variable delay in place of single pulse excitation, we show controlled fluorescence excitation or suppression of one of the fluorophores over the other through wave-packet interferometry. Such an effect prevails even after the fluorophore coherence timescale, which effectively results in a higher spatial resolution. Here we extend the effect of our pulse-pair technique to microscopic axial resolution experiments and show that such pairs of pulses can also ‘enhance’ axial resolution.

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