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Cai, Na. "Molecular markers of stress". Thesis, University of Oxford, 2015. https://ora.ox.ac.uk/objects/uuid:95826e79-6ef0-4148-8478-5778994f97fc.
Pełny tekst źródłaPinto, Diana Maria de Figueiredo. "Molecular markers for diabetic nephropathy". Master's thesis, Universidade de Aveiro, 2015. http://hdl.handle.net/10773/15953.
Pełny tekst źródłaType 2 diabetes is one of the most common metabolic disorders in the world. Globally, the prevalence of this disorder is predicted to increase, along with the risk of developing diabetic related complications. One of those complications is diabetic nephropathy, defined by a progressive increase in proteinuria and a gradual decline in renal function. Approximately 25% to 30% of type 2 diabetic individuals develop this complication. However, its underlying genetic mechanisms remain unclear. Thus, the aim of this study is to contribute to the discovery of the genetic mechanisms involved in the development and progression of diabetic nephropathy, through the identification of relevant genetic variants in Portuguese type 2 diabetic individuals. The exomes of 36 Portuguese type 2 diabetic individuals were sequenced on the Ion ProtonTM Sequencer. From those individuals, 19 did not present diabetic nephropathy, being included in the control group, while the 17 individuals that presented the diabetic complication formed the case group. A statistical analysis was then performed to identify candidate common genetic variants, as well as genes accumulating rare variants that could be associated with diabetic nephropathy. From the search for common variants in the study population, the statistically significant (p-value ≤ 0.05) variants rs1051303 and rs1131620 in the LTBP4 gene, rs660339 in UCP2, rs2589156 in RPTOR, rs2304483 in the SLC12A3 gene and rs10169718 present in ARPC2, were considered as the most biologically relevant to the pathogenesis of diabetic nephropathy. The variants rs1051303 and rs1131620, as well as the variants rs660339 and rs2589156 were associated with protective effects in the development of the complication, while rs2304483 and rs10169718 were considered risk variants, being present in individuals with diagnosed diabetic nephropathy. In the rare variants approach, the genes with statistical significance (p-value ≤ 0.05) found, the STAB1 gene, accumulating 9 rare variants, and the CUX1 gene, accumulating 2 rare variants, were identified as the most relevant. Both genes were considered protective, with the accumulated rare variants mainly present in the group without the renal complication. The present study provides an initial analysis of the genetic evidence associated with the development and progression of diabetic nephropathy, and the results obtained may contribute to a deeper understanding of the genetic mechanisms associated with this diabetic complication.
A diabetes tipo 2 é um dos distúrbios metabólicos mais comuns no mundo. Globalmente, está previsto um aumento da sua prevalência, assim como um aumento do risco de desenvolver complicações associadas. Uma dessas complicações é a nefropatia diabética, definida pelo aumento progressivo de proteinúria e um declínio gradual da função renal. Aproximadamente 25% a 30% dos indivíduos com diabetes tipo 2 desenvolvem esta complicação. No entanto, os mecanismos genéticos associados permanecem por esclarecer. Posto isto, o objetivo deste estudo é contribuir para a identificação dos mecanismos envolvidos no desenvolvimento e progressão desta complicação, através da identificação de variantes genéticas relevantes, em indivíduos com diabetes tipo 2 na população portuguesa. Para isso, os exomas de 36 portugueses com diabetes tipo 2 foram sequenciados na plataforma Ion ProtonTM. Desses individuos, 19 não apresentavam nefropatia diabética, tendo sido incluídos no grupo de controlo, e os restantes 17 individuos, com a complicação diagnosticada, formaram o grupo dos casos. Uma análise estatística foi depois realizada para identificar, com base nas diferenças genéticas entre os dois grupos, variantes comuns, assim como genes que acumulam variantes raras candidatas, que podem explicar o risco acrescido ou diminuído para desenvolver a complicação. Na pesquisa das variantes comuns, as variantes rs1051303 e o rs1131620 no gene LTBP4, a variante rs660339 no UCP2, a variante rs2589156 no gene RPTOR, a variante rs2304483 no SLC12A3 e, por fim, a variante rs10169718 presente no gene ARPC2, foram, de todas aquelas consideradas estatisticamente significativas (p-value ≤ 0,05), as mais relevantes para a patogénese da nefropatia diabética. O rs1051303 e o rs1131620, assim como o rs660339 e o rs2589156, têm um efeito protetor, enquanto o rs2304483 e o rs10169718 foram considerados de risco, estando associados a indivíduos que sofrem da complicação referida. Pela abordagem utilizada para identificar as variantes raras, o gene STAB1, que acumula 9 variantes, e o gene CUX1, que acumula 2, foram, de todos os genes com significado estatístico (p-value ≤ 0,05), aqueles que se evidenciaram como sendo biologicamente relevantes. Ambos os genes foram considerados protetores, já que as suas variantes raras acumuladas estavam presentes maioritariamente nos indivíduos que não apresentam esta complicação renal. Este estudo providencia uma análise inicial das evidências genéticas associadas ao desenvolvimento e progressão da nefropatia diabética, podendo os seus reultados contribuir para uma melhor compreensão dos mecanismos genéticos que estão por detrás do seu surgimento.
Weigelt, Britta. "Molecular markers of breast cancer metastasis". [S.l. : Amsterdam : s.n.] ; Universiteit van Amsterdam [Host], 2005. http://dare.uva.nl/document/88848.
Pełny tekst źródłaSwagell, Christopher Dean. "Molecular markers of obesity and diabetes". Thesis, Queensland University of Technology, 2007. https://eprints.qut.edu.au/35762/1/Christopher_Swagell_Thesis.pdf.
Pełny tekst źródłaBitalo, Daphne Nyachaki. "Implementation of molecular markers for triticale cultivar identification and marker-assisted selection". Thesis, Stellenbosch : Stellenbosch University, 2012. http://hdl.handle.net/10019.1/71670.
Pełny tekst źródłaTriticale is an amphidiploid that consists of wheat (A and B) and rye (R) genomes. This cereal is fast becoming important on a commercial basis and warrants further assessment for the better management and breeding of the hybrid. The assessment of the genetic diversity among the wheat and rye genomes within triticale can be obtained by using molecular markers developed in both donor genomes. Simple sequence repeats markers (SSRs) and amplified fragment length markers (AFLPs) have been previously used to assess the genetic diversity among triticale lines. SSRs are highly polymorphic markers that are abundant and which have been shown to be highly transferable between species in previous studies while AFLP markers are known to generate plenty of data as they cover so many loci. Thus, the aim of this study was to develop a marker system suitable to assess the genetic diversity and relationships of advanced breeding material (and cultivars) of the Stellenbosch University’s Plant Breeding Laboratory (SU-PBL). Therefore, both AFLP and SSR markers were initially analysed using eight triticale cultivars (with known pedigrees) to facilitate cultivar identification. Fourty-two AFLP primer combinations and 86 SSR markers were used to assess the genetic diversity among the Elite triticale cultivars. The AFLP primer combinations generated under average polymorphism information content (PIC) values. Furthermore, these markers generated neighbour-joining (NJ) and unweighted pair group method with arithmetic average (UPGMA) dendograms that displayed relationships that did not correspond with the available pedigree information. Therefore, this marker system was found not to be suitable. A set of 86 SSRs previously identified in both wheat and rye, was used to test the genetic diversity among the eight cultivars. The markers developed in wheat achieved 84% transferability while those developed in rye achieved 79.3% transferability. A subset of SSR markers was able to distinguish the cultivars, and correctly identify them by generating NJ and UPGMA dendograms that exhibited relationships that corroborated the available pedigree data. This panel of markers was therefore chosen as the most suitable for the assessment of the advanced breeding material. The panel of seven SSR markers was optimised for semi-automated analysis and was used to screen and detect the genetic diversity among 306 triticale entries in the F6, Senior and Elite phases of the SU-PBL triticale breeding programme. An average PIC value of 0.65 was detected and moderate genetic variation was observed. NJ and UPGMA dendograms generated showed no clear groupings. However, the panel of markers managed to accurately identify all cultivars within the breeding program. The marker panel developed in this study is being used to routinely distinguish among the advanced breeding material within the SU-PBL triticale breeding programme and as a tool in molecular-assisted backcross.
Valdman, Alexander. "Molecular genetic markers of prostate cancer development /". Stockholm, 2003. http://diss.kib.ki.se/2003/91-7349-618-9/.
Pełny tekst źródłaLarkin, Samantha. "Molecular characterisation of prostate cancer progression markers". Thesis, University of Portsmouth, 2010. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.511205.
Pełny tekst źródłaSteele, Katherine A. "Molecular markers in yellow rust of wheat". Thesis, University of Nottingham, 1997. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.243712.
Pełny tekst źródłaHemmings, Karen Emily. "Cellular and molecular markers of oocyte quality". Thesis, University of Leeds, 2007. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.445945.
Pełny tekst źródłaLaird, Alexander. "Molecular prognostic markers in renal cell carcinoma". Thesis, University of Edinburgh, 2015. http://hdl.handle.net/1842/17873.
Pełny tekst źródłaCooper, Grace. "Molecular Markers of Alzheimer’s and Parkinson’s Diseases". The Ohio State University, 2018. http://rave.ohiolink.edu/etdc/view?acc_num=osu1523975395945636.
Pełny tekst źródłaFarr, Ryan. "Molecular Markers of Pancreatic β-cell Death". Thesis, The University of Sydney, 2017. http://hdl.handle.net/2123/17308.
Pełny tekst źródłaSarela, Abeezar Ismail. "Molecular genetic markers of prognosis in colorectal carcinoma". Thesis, University of Leeds, 2006. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.438485.
Pełny tekst źródłaCao, Wenguang. "Wheat taxonomy and cultivar identification using molecular markers". Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1997. http://www.collectionscanada.ca/obj/s4/f2/dsk3/ftp04/nq24007.pdf.
Pełny tekst źródłaStoltzfus, Patricia. "Molecular markers reflecting malignant transformation and tumor progression /". Stockholm, 2004. http://diss.kib.ki.se/2004/91-7349-888-2.
Pełny tekst źródłaTascilar, Metin. "Clinical significance of molecular markers in pancreatic cancer". [S.l. : Amsterdam : s.n.] ; Universiteit van Amsterdam [Host], 2002. http://dare.uva.nl/document/61858.
Pełny tekst źródłaHopwood, N. D. "Molecular markers of mesoderm induction in Xenopus laevis". Thesis, University of Cambridge, 1989. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.317830.
Pełny tekst źródłaKawesha, Anthony. "Prognostic molecular markers in resected ductal pancreatic carcinoma". Thesis, University of Birmingham, 2017. http://etheses.bham.ac.uk//id/eprint/7596/.
Pełny tekst źródłaHu, Jiahuai. "Phytophthora nicotianae: Fungicide Sensitivity, Fitness, and Molecular Markers". Diss., Virginia Tech, 2007. http://hdl.handle.net/10919/26416.
Pełny tekst źródłaPh. D.
au, Constantine@wehi edu, i Clare Constantine. "Molecular markers, analysis and the population genetics of parasites". Murdoch University, 2002. http://wwwlib.murdoch.edu.au/adt/browse/view/adt-MU20050817.102006.
Pełny tekst źródłaConstantine, Clare. "Molecular markers, analysis and the population genetics of parasites". Constantine, Clare (2002) Molecular markers, analysis and the population genetics of parasites. PhD thesis, Murdoch University, 2002. http://researchrepository.murdoch.edu.au/662/.
Pełny tekst źródłaHoerder, Anna. "Mouse cortical subplate neurones : molecular markers, connectivity and development". Thesis, University of Oxford, 2007. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.442449.
Pełny tekst źródłaConway, Caroline Anne. "Identification of molecular markers of prognosis in malignantm melanoma". Thesis, University of Leeds, 2008. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.496124.
Pełny tekst źródłaCheung, Nga-yin Annie, i 張雅賢. "Cervical cancer screening: evolution from Paptest to molecular markers". Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2011. http://hub.hku.hk/bib/B46540465.
Pełny tekst źródłaChapman, Natalie HeÌleÌ€ne. "Identification and mapping of molecular markers for eyespot resistance". Thesis, University of East Anglia, 2005. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.426603.
Pełny tekst źródłaLeal, Soraya Cristina de Macedo. "Detection and characterization of Metarhizium anisopliae using molecular markers". Thesis, University of Nottingham, 1996. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.307762.
Pełny tekst źródłaHall, Nicola. "Stable isotopes, molecular markers and water use in Brassicas". Thesis, University of Newcastle Upon Tyne, 2000. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.324874.
Pełny tekst źródłaAllender, Charlotte Jane. "Molecular markers and the speciation of African cichlid fish". Thesis, University of Southampton, 2001. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.394101.
Pełny tekst źródłaBurrows, Kimberley. "Molecular genetic epidemiology studies of quantitative nucleic acid markers". Thesis, University of Bristol, 2015. https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.761240.
Pełny tekst źródłaPlata-Muñoz, Juan José. "Clinical, biochemical and molecular markers of injury before transplantation". Thesis, University of Oxford, 2012. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.572681.
Pełny tekst źródłaSchrag, Tobias A. "Prediction of hybrid performance in maize using molecular markers". [S.l. : s.n.], 2008. http://nbn-resolving.de/urn:nbn:de:bsz:100-opus-3035.
Pełny tekst źródłaPoli, Maurizio. "Novel molecular markers for assessment of human embryo competence". Thesis, University of Oxford, 2016. http://ora.ox.ac.uk/objects/uuid:4c5bffff-d12c-4df1-b5f0-1459298fc45c.
Pełny tekst źródłaConstantine, Clare Colleen. "Molecular markers, analysis and the population genetics of parasites". Thesis, Constantine, Clare Colleen (2002) Molecular markers, analysis and the population genetics of parasites. PhD thesis, Murdoch University, 2002. https://researchrepository.murdoch.edu.au/id/eprint/662/.
Pełny tekst źródłaConstantine, Clare Colleen. "Molecular markers, analysis and the population genetics of parasites /". Access via Murdoch University Digital Theses Project, 2002. http://wwwlib.murdoch.edu.au/adt/browse/view/adt-MU20050817.102006.
Pełny tekst źródłaAmbrosini, Spaltro Andrea <1978>. "Immunohistochemical and Molecular Prognostic/Predictive Markers in Neoplastic Diseases". Doctoral thesis, Alma Mater Studiorum - Università di Bologna, 2012. http://amsdottorato.unibo.it/4309/1/ambrosinispaltro_andrea_tesi.pdf.
Pełny tekst źródłaAmbrosini, Spaltro Andrea <1978>. "Immunohistochemical and Molecular Prognostic/Predictive Markers in Neoplastic Diseases". Doctoral thesis, Alma Mater Studiorum - Università di Bologna, 2012. http://amsdottorato.unibo.it/4309/.
Pełny tekst źródłaBERZERO, GIULIA. "“MOLECULAR MARKERS IN DIFFUSE GLIOMAS: DIAGNOSTIC AND THERAPEUTIC TARGETS”". Doctoral thesis, Università degli studi di Pavia, 2020. http://hdl.handle.net/11571/1371676.
Pełny tekst źródłaAMOROSO, ALFREDO. "Development of new molecular markers in immunohematology and oncology". Doctoral thesis, Università del Piemonte Orientale, 2014. http://hdl.handle.net/11579/58206.
Pełny tekst źródłaLA, CORTE EMANUELE. "CLINICAL AND MOLECULAR BIO-MARKERS IN SKULL BASE CHORDOMAS". Doctoral thesis, Università degli Studi di Milano, 2019. http://hdl.handle.net/2434/610397.
Pełny tekst źródłaIntroduction. Skull base chordomas are rare slow-growing neoplasms that arise from notochord. Their morbidity is mainly related to highly aggressive local invasion and resistance to treatments. Due to its heterogeneous appearance and not fully understood clinical and molecular behaviors, the main goal of the present work is to identify clinical and bio-molecular markers as specific prognostic factors that could be used for the management of skull base chordoma patients. Achieving a detailed prognostic signature of skull base chordomas is of paramount importance to personalize the treatment to each specific patient. Moreover, sphingolipids analysis is emerging as a new approach in many cancers and it has never been applied in chordomas. Our aim is to investigate chordoma biological behavior and the role of ceramides production in this context of proliferation and invasion. Patients and Methods. A retrospective review of all the patients diagnosed and treated for a skull base chordoma at the Fondazione IRCCS Istituto Neurologico “Carlo Besta” between January 1992 and December 2017 has been performed. Clinical, radiological, surgical and pathological data have been collected. A prospective collection of frozen surgical specimens has been performed to analyze ceramides species in chordomas. Sphingolipids were extracted from frozen tissues and total ceramides and dihydroceramides were evaluated by liquid chromatography and mass spectrometry. Survival analysis was performed according to Kaplan-Meier method. Univariate comparisons were conducted using Mann-Whitney, Chi-square and exact Fisher test. Simple linear regression and correlation with computation of Pearson coefficients analyses were conducted. Using a logistic regression model, statistically significant predictors were rated based on their odds ratios in order to build a personalized grading scale – the Peri-Operative Chordoma Scale (POCS). Results. Eighty-seven consecutive patients were surgically treated for a skull base chordoma during the period of recruitment. Seventy-eight patients were eligible for the retrospective review. There were 38 males (48.7%) and 40 females (51.3%). The mean follow-up was 69 months (range, 3–233). One-hundred-fourteen surgical operations were performed in the initial recruitment or recurrent setting. The presence of motor deficits in skull base chordoma revealed to be a significant prognostic factor correlating with a worse PFS (p=0.0480). Calcification on KM analysis showed a correlation with better outcomes (OS) compared with tumor lacking any calcification on CT scan (p value=0.0402). The degree of MR contrast enhancement revealed to be a significant and strong prognostic factor in terms of OS and PFS (p≤0.0001 and 0.0010, respectively). Jugular foramen involvement represented a significant prognostic factor with a worse PFS in the cohort of primary skull base chordomas (p=0.0130). The presence of chordoma in the pre-brainstem cistern revealed to be a significant prognostic factor with a worse PFS in the cohort of recurrent skull base chordomas (p=0.0210). Brainstem dislocation represented a significant prognostic factor correlating with a both worse outcome in terms of OS and PFS in the cohort of recurrent skull base chordomas (p=0.0060 and 0.0030). Extent of resection represents a strong prognostic factor according to PFS in the cohort of primary skull base chordomas (p=0.0200). Patients operated by an experienced chordoma surgeon did better in terms of prolonged PFS in the cohort of primary patients (p=0.0340). Development of post-operative complications in primary skull base chordoma patients represented an important prognostic factor related to both OS and PFS (p≤0.0001 and 0.0360, respectively). In the cohort of recurrent chordomas, ∆KPS correlated to both OS and PFS (p=0.0010 and 0.0180, respectively). Moreover, post-operative radiation treatment correlated with prolonged OS (p=0.0020) and PFS (p=0.0100). The following factors were found to be statistically significant predictors of both PFS and OS in the logistic regression model: MR contrast enhancement (intense vs mild/no), preoperative motor deficit (yes vs no) and the development of any post-operative complications (yes vs no). A grading scale was obtained with scores ranging between 0 and 17 (Nagelkerke’s pseudo R2=0.656). The mean total ceramides and dihydroceramides species in primary chordomas were 808.4±451.4 pmol/mg (522.5-1760.2) and 30.7±16.4 pmol/mg (17.6-62.4), respectively. The mean total ceramides and dihydroceramides species in recurrent chordomas were 1488.1±763.8 pmol/mg (540.7-2787.5) and 67.2±45.5 pmol/mg (9.0-145.6), respectively. Total ceramides species were significantly higher in recurrent chordomas that underwent previous surgical resection and radiation therapy in comparison to the primary chordomas (p=0.0496). The mean total ceramides and dihydroceramides species in “intense enhancement” group were 1597.6±737.8 pmol/mg (592.7-2787.5) and 69.1±45.0 pmol/mg (17.8-145.6), respectively. The mean total ceramides and dihydroceramides species in “no or mild enhancement” group were 664.7±120.4 pmol/mg (522.5-826.0) and 31.5±13.6 pmol/mg (17.6-53.6), respectively. Total ceramides and dihydroceramides were significantly higher in “intense enhancement” chordomas in comparison to the “no/mild enhancement” chordomas (p=0.0290 and p=0.0186, respectively). Analyzing the association between ceramides level and MIB-1 within each skull base chordoma patient, total ceramides level showed a strong association (r=0.7257, r2=0.5267) with MIB-1 staining (p=0.0033). Analyzing the association between DHCer level and MIB-1 within each skull base chordoma patient, total DHCer level showed also strong association (r=0.6733, r2= 0.4533) with MIB-1 staining (p= 0.0083). Among the single ceramides species Cer C24:1 (r=0.8814, r2=0.7769, p≤0.0001), DHCer C24:1 (r=0.8429, r2=0.7104, p=0.0002) and DHCer C18:0 (r=0.9426, r2=0.8885, p≤0.0001) levels showed a significant correlation with MIB-1 staining. Final candidate predictive factors that well fitted the regression model were: cer24:1 (r=0.824, p≤0.001), and DHCer C18:0 (r=0.748, p=0.002). Conclusion. Our clinical analysis showed that pre-operative clinical symptoms (motor and cranial nerve deficits), anatomical location (jugular foramen, pre-brainstem cisterns and brainstem dislocation), surgical features (extent of tumor resection and surgeon’s experience), development of post-operative complications and KPS decline represent significant prognostic factors. The degree of MR contrast enhancement significantly correlated to both OS and PFS. We also preliminarily developed the Peri-Operative Chordoma Scale (POCS) to aid the practitioner in the personalized management of patients undergoing potential adjuvant therapies. Our lipid analysis showed ceramides as promising tumoral bio-markers in skull base chordomas. Long and very long chain ceramides, such as Cer C24:1 and DHCer C24:1, may be related to a prolonged tumor survival, aggressiveness and the understanding of their effective biological role will hopefully shed lights on the mechanisms of chordoma radio-resistance, tendency to recur and use of agents targeting ceramide metabolism. Such results should be validated in future larger clinical, in-vitro and in-vivo studies to confirm such intricate link between ceramides and chordoma aggressive behavior.
Ishiy, Felipe Augusto André. "Evaluation of molecular markers in osteogenic differentiation of mesenchymal stem cells". Universidade de São Paulo, 2016. http://www.teses.usp.br/teses/disponiveis/41/41131/tde-20032017-104921/.
Pełny tekst źródłaO uso de células-tronco trata-se de uma abordagem terapêutica promissora para a engenharia de tecidos, devido à sua capacidade na regeneração de tecidos, e para modelamento in vitro de distúrbios genéticos humanos, uma vez que fornece um abastecimento contínuo de células com potencial de diferenciação. Nosso estudo se propos a identificar moléculas e mecanismos que contribuem na otimização da osteogênese de células-tronco mesenquimais (MSCs). Para atingir nossos objetivos exploramos as diferenças no potencial osteogênico (PO) de MSCs de diferentes fontes. Observamos que MSCs de polpa de dente decíduo humano (SHED) apresentaram maior PO em comparação com as MSC derivadas de tecido adiposo humano (hASCs). Através de análise de microarray de expressão e cell sorting, demonstramos que os níveis de expressão de IGF2 e CD105 contribuem para as diferenças do PO, onde a maior expressão de IGF2 e menor expressão de CD105 estão associadas a maior PO em SHED quando comparado as hASCs. Também investigamos os mecanismos moleculares associados aos diferentes níveis de expressão de IGF2 E CD105 em ambas as fontes celulares. Apesar das vantagens, as MSCs podem apresentar pontos negativos como restrita auto-renovação e menor quantidade de células. Células-tronco pluripotentes induzidas (iPSC) surgem como uma fonte celular alternativa, proporcionando populações celulares homogêneas com auto-renovação prolongada e maior plasticidade. O PO de MSC-like iPSC difere de MSCs, e este potencial é dependente da fonte celular em que as iPSCs são obtidas. Análise comparativa de PO in vitro demonstrou maior osteogênse em células MSC-like derivadas de iPS-SHED quando comparada as células MSC-like de iPSCs-fibroblastos e SHED. iPSCs também podem ser utilizadas como ferramenta para investigar doenças genéticas humanas. Propomos a modelagem in vitro da síndrome de Treacher-Collins (TSC), doença que acomete as estruturas craniofaciais durante o desenvolvimento ósseo. Comparamos os efeitos de mutações patogênicas no gene TCOF1 na proliferação celular, potencial de diferenciação entre MSCs, fibroblastos dérmicos, neural-crest like e células MSC-like diferenciadas de iPSCs. Células de pacientes TCS exibiram alterações em propriedades celulares e na expressão de marcadores osteogênicos e condrogênicos. Em resumo, a análise comparativa de células-tronco de diferentes fontes permitiu a identificação de marcadores e mecanismos que podem facilitar a osteogênese e tambem demonstramos que é possível modelar in vitro a síndrome de Treacher-Collins
Bernal, Sierra Yinth Andrea [Verfasser]. "Molecular markers of mechanoreceptors and potential molecules for gating mechanotransduction / Yinth Andrea Bernal Sierra". Berlin : Freie Universität Berlin, 2013. http://d-nb.info/1045194972/34.
Pełny tekst źródłaJung, Renata. "Identification of Molecular Markers for Marker-Assisted Selection of Malting Quality and Associated Traits in Barley". Diss., North Dakota State University, 2015. http://hdl.handle.net/10365/25241.
Pełny tekst źródłaAmerican Malting Barley Association
Poulsen, David Malcolm Ernest. "Application of molecular markers to breeding barleys for disease resistance /". St. Lucia, Qld, 2002. http://www.library.uq.edu.au/pdfserve.php?image=thesisabs/absthe17378.pdf.
Pełny tekst źródłaBin, Kaderi Mohamed Arifin. "Assessment of Novel Molecular Prognostic Markers in Chronic Lymphocytic Leukemia". Doctoral thesis, Uppsala universitet, Hematologi och immunologi, 2010. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-110371.
Pełny tekst źródłaLaatio, L. (Liisa). "In search of new prognostic molecular markers in ovarian cancer". Doctoral thesis, Oulun yliopisto, 2012. http://urn.fi/urn:isbn:9789514298349.
Pełny tekst źródłaTiivistelmä Munasarjasyöpä on suurinta kuolleisuutta aiheuttava gynekologinen syöpä läntisessä maailmassa. Munasarjakasvaimet eroavat toisistaan niin käyttäytymiseltään kuin yksilölliseltä sytostaattihoitovasteeltaan, jopa sama histologisen tyypin sisällä. Kliinisessä käytössä ei valitettavasti ole sellaisia molekulaarisia merkkiaineita, jotka erottaisivat toisistaan paremman ja huonomman ennusteen kasvaimet tai ennustaisivat yksilöllistä solunsalpaajaherkkyyttä. Hoitovastetta ja potilaan prognoosia ennustavien merkkiaineiden löytämisen edellytys on kemoterapian molekyylitason vaikutusten ymmärtäminen. DNA vaurion tunnistamiseen, angiogeneesiin ja oksidatiiviseen stressiin liittyvien vaikutusreittien joillakin proteiineilla on ehdotettu olevan ennusteellista merkitystä munasarjasyövässä. Tässä väitöskirjatyössä analysoitiin munasarjasyöpäsoluja käyttäen p53 vaikutusreitin ja eräiden angiogeneesiin liittyvien tekijöiden vasteita sytostaateille. Mutatoitunutta p53 proteiinia kantavissa OVCAR-3 soluissa sisplatiini, toisin kuin dosetakseli, indusoi p53 proteiinin tärkeää säätelijää, p14ARF:a sekä mRNA- että proteiinitasolla. Sisplatiini lisäsi merkittävästi myös usean angiogeneesiin liittyvän tekijän (TSP-1, BMP-4, ET-1 ja PlGF-2) mRNA:ta. Dosetakselin vaikutukset vastaavalla annoksella olivat vähäiset. Kliinisissä munasarjasyövissä BMP-4, TSP-1 ja CD105 sekä oksidatiivisen stressin aiheuttaman DNA-vaurion merkkiaineen, 8-OHdG:n sekä peroksiredoksiiniantioksidanttien ilmeneminen analysoitiin immunohistokemiallisesti. BMP-4:n ja sytoplasmisen peroksiredoksiini IV:n vahva ilmentyminen liittyivät parempaan ennusteeseen, kun taas 8-OHdG:n vahva ilmentyminen liittyi huonompaan elinajan ennusteeseen. Tuoreen munasarjasyöpäkudoksen eksplanttiviljelyn avulla selvitettiin p53 ja Hdm2 proteiinien vasteita syöpäkudoksen karboplatiini- tai dosetakseli-käsittelyille. Selkeitä yksilökohtaisia eroja havaittiin erityisesti karboplatiinin aiheuttamissa p53 vasteissa niin eri potilaiden kuin eri histologisten kasvaintyyppien välillä. Tämän väitöskirjatutkimuksen tulokset antavat viitteitä BMP-4:n, 8-OHdG:n ja peroksiredoksiinin mahdollisesta ennusteellisesta merkityksestä munasarjasyövässä. Erot platinayhdisteiden ja taksaanien välillä saattavat osoittautua merkittäviksi yksilöllisiä syövän hoitoja räätälöitäessä. Tuoreen munasarjasyöpäkudoksen eksplanttiviljelyn mahdollisuuksia yksilöllisten kasvainten hoitovasteiden ennustamisessa kannattaa selvittää jatkotutkimuksin
Scullin, P. "Molecular markers of response to systemic therapy in prostate cancer". Thesis, Queen's University Belfast, 2008. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.492490.
Pełny tekst źródłaPonza, Pattareeya, i pattareeya pon@biotec or th. "Molecular markers of ecotoxicological interest in the rainbowfish Melanotaenia fluviatilis". RMIT University. Applied Science, 2007. http://adt.lib.rmit.edu.au/adt/public/adt-VIT20080102.121231.
Pełny tekst źródłaTar'an, Bunyamin. "Development and application of molecular markers in common bean breeding". Thesis, National Library of Canada = Bibliothèque nationale du Canada, 2000. http://www.collectionscanada.ca/obj/s4/f2/dsk1/tape2/PQDD_0015/NQ47413.pdf.
Pełny tekst źródłaLuan, Shi-Lu. "Molecular epidemiology of Streptococcus agalactiae : mobile elements as genetic markers /". Doctoral thesis, Umeå : Umeå University, 2006. http://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-877.
Pełny tekst źródłaMazhar, Kehkashan. "Molecular genetic markers for selection and genome mapping in cattle". Thesis, University of Reading, 1994. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.260797.
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