Artykuły w czasopismach na temat „Microfluidic devices”
Kliknij ten link, aby zobaczyć inne rodzaje publikacji na ten temat: Microfluidic devices.
Utwórz poprawne odniesienie w stylach APA, MLA, Chicago, Harvard i wielu innych
Sprawdź 50 najlepszych artykułów w czasopismach naukowych na temat „Microfluidic devices”.
Przycisk „Dodaj do bibliografii” jest dostępny obok każdej pracy w bibliografii. Użyj go – a my automatycznie utworzymy odniesienie bibliograficzne do wybranej pracy w stylu cytowania, którego potrzebujesz: APA, MLA, Harvard, Chicago, Vancouver itp.
Możesz również pobrać pełny tekst publikacji naukowej w formacie „.pdf” i przeczytać adnotację do pracy online, jeśli odpowiednie parametry są dostępne w metadanych.
Przeglądaj artykuły w czasopismach z różnych dziedzin i twórz odpowiednie bibliografie.
1
Ballacchino, Giulia, Edward Weaver, Essyrose Mathew, Rossella Dorati, Ida Genta, Bice Conti i Dimitrios A. Lamprou. "Manufacturing of 3D-Printed Microfluidic Devices for the Synthesis of Drug-Loaded Liposomal Formulations". International Journal of Molecular Sciences 22, nr 15 (28.07.2021): 8064. http://dx.doi.org/10.3390/ijms22158064.
Pełny tekst źródłaStreszczenie:
Microfluidic technique has emerged as a promising tool for the production of stable and monodispersed nanoparticles (NPs). In particular, this work focuses on liposome production by microfluidics and on factors involved in determining liposome characteristics. Traditional fabrication techniques for microfluidic devices suffer from several disadvantages, such as multistep processing and expensive facilities. Three-dimensional printing (3DP) has been revolutionary for microfluidic device production, boasting facile and low-cost fabrication. In this study, microfluidic devices with innovative micromixing patterns were developed using fused deposition modelling (FDM) and liquid crystal display (LCD) printers. To date, this work is the first to study liposome production using LCD-printed microfluidic devices. The current study deals with 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC) liposomes with cholesterol (2:1) prepared using commercial and 3D-printed microfluidic devices. We evaluated the effect of microfluidic parameters, chip manufacturing, material, and channel design on liposomal formulation by analysing the size, PDI, and ζ-potential. Curcumin exhibits potent anticancer activity and it has been reported that curcumin-loaded liposomes formulated by microfluidics show enhanced encapsulation efficiency when compared with other reported systems. In this work, curcumal liposomes were produced using the developed microfluidic devices and particle sizing, ζ-potential, encapsulation efficiency, and in vitro release studies were performed at 37 °C.
2
Cai, Jianchen, Jiaxi Jiang, Jinyun Jiang, Yin Tao, Xiang Gao, Meiya Ding i Yiqiang Fan. "Fabrication of Transparent and Flexible Digital Microfluidics Devices". Micromachines 13, nr 4 (23.03.2022): 498. http://dx.doi.org/10.3390/mi13040498.
Pełny tekst źródłaStreszczenie:
This study proposed a fabrication method for thin, film-based, transparent, and flexible digital microfluidic devices. A series of characterizations were also conducted with the fabricated digital microfluidic devices. For the device fabrication, the electrodes were patterned by laser ablation of 220 nm-thick indium tin oxide (ITO) layer on a 175 μm-thick polyethylene terephthalate (PET) substrate. The electrodes were insulated with a layer of 12 μm-thick polyethylene (PE) film as the dielectric layer, and finally, a surface treatment was conducted on PE film in order to enhance the hydrophobicity. The whole digital microfluidic device has a total thickness of less than 200 μm and is nearly transparent in the visible range. The droplet manipulation with the proposed digital microfluidic device was also achieved. In addition, a series of characterization studies were conducted as follows: the contact angles under different driving voltages, the leakage current density across the patterned electrodes, and the minimum driving voltage with different control algorithms and droplet volume were measured and discussed. The UV–VIS spectrum of the proposed digital microfluidic devices was also provided in order to verify the transparency of the fabricated device. Compared with conventional methods for the fabrication of digital microfluidic devices, which usually have opaque metal/carbon electrodes, the proposed transparent and flexible digital microfluidics could have significant advantages for the observation of the droplets on the digital microfluidic device, especially for colorimetric analysis using the digital microfluidic approach.
3
Zhu, Zhiyuan, Fan Zeng, Zhihua Pu i Jiyu Fan. "Conversion Electrode and Drive Capacitance for Connecting Microfluidic Devices and Triboelectric Nanogenerator". Electronics 12, nr 3 (19.01.2023): 522. http://dx.doi.org/10.3390/electronics12030522.
Pełny tekst źródłaStreszczenie:
Microfluidics is a technique that uses channels of tiny sizes to process small amounts of fluid, which can be used in biochemical detection, information technology, and other fields. In the process of microfluidic development, there are many problems that need to be solved urgently. Many microfluidic systems require the support of external devices, which increases the construction cost, and the electronic interface technology is not mature. A triboelectric nanogenerator (TENG) can harvest mechanical energy and turn it into electrical energy. It has been greatly developed now and is widely used in various fields. Nowadays, many studies are committed to the study of TENGs and microfluidic systems. The microfluidics device can be combined with a TENG to convert fluid mechanical signals into electrical signals for transmission. Meanwhile, TENGs can also act as a high-voltage source to drive microfluidic motion. In this paper, we reviewed the development of microfluidics and related technologies of microfluidic systems in conjunction with TENGs and discussed the form of electronic interface between microfluidic systems and TENG devices.
4
Kurniawan, Yehezkiel Steven, Arif Cahyo Imawan, Sathuluri Ramachandra Rao, Keisuke Ohto, Wataru Iwasaki, Masaya Miyazaki i Jumina. "Microfluidics Era in Chemistry Field: A Review". Journal of the Indonesian Chemical Society 2, nr 1 (31.08.2019): 7. http://dx.doi.org/10.34311/jics.2019.02.1.7.
Pełny tekst źródłaStreszczenie:
By miniaturizing the reactor dimension, microfluidic devices are attracting world attention and starting the microfluidic era, especially in the chemistry field because they offer great advantages such as rapid processes, small amount of the required reagents, low risk, ease and accurate control, portable and possibility of online monitoring. Because of that, microfluidic devices have been massively investigated and applied for the real application of human life. This review summarizes the up-to-date microfluidic research works including continuous-flow, droplet-based, open-system, paper-based and digital microfluidic devices. The brief fabrication technique of those microfluidic devices, as well as their potential application for particles separation, solvent extraction, nanoparticle fabrication, qualitative and quantitative analysis, environmental monitoring, drug delivery, biochemical assay and so on, are discussed. Recent perspectives of the microfluidics as a lab-on-chip or micro total analysis system device and organ-on-chip are also introduced.
5
Tang, Xiaoqing, Qiang Huang, Tatsuo Arai i Xiaoming Liu. "Cell pairing for biological analysis in microfluidic devices". Biomicrofluidics 16, nr 6 (grudzień 2022): 061501. http://dx.doi.org/10.1063/5.0095828.
Pełny tekst źródłaStreszczenie:
Cell pairing at the single-cell level usually allows a few cells to contact or seal in a single chamber and provides high-resolution imaging. It is pivotal for biological research, including understanding basic cell functions, creating cancer treatment technologies, developing drugs, and more. Laboratory chips based on microfluidics have been widely used to trap, immobilize, and analyze cells due to their high efficiency, high throughput, and good biocompatibility properties. Cell pairing technology in microfluidic devices provides spatiotemporal research on cellular interactions and a highly controlled approach for cell heterogeneity studies. In the last few decades, many researchers have emphasized cell pairing research based on microfluidics. They designed various microfluidic device structures for different biological applications. Herein, we describe the current physical methods of microfluidic devices to trap cell pairs. We emphatically summarize the practical applications of cell pairing in microfluidic devices, including cell fusion, cell immunity, gap junction intercellular communication, cell co-culture, and other applications. Finally, we review the advances and existing challenges of the presented devices and then discuss the possible development directions to promote medical and biological research.
6
Männel, Max J., Elif Baysak i Julian Thiele. "Fabrication of Microfluidic Devices for Emulsion Formation by Microstereolithography". Molecules 26, nr 9 (10.05.2021): 2817. http://dx.doi.org/10.3390/molecules26092817.
Pełny tekst źródłaStreszczenie:
Droplet microfluidics—the art and science of forming droplets—has been revolutionary for high-throughput screening, directed evolution, single-cell sequencing, and material design. However, traditional fabrication techniques for microfluidic devices suffer from several disadvantages, including multistep processing, expensive facilities, and limited three-dimensional (3D) design flexibility. High-resolution additive manufacturing—and in particular, projection micro-stereolithography (PµSL)—provides a promising path for overcoming these drawbacks. Similar to polydimethylsiloxane-based microfluidics 20 years ago, 3D printing methods, such as PµSL, have provided a path toward a new era of microfluidic device design. PµSL greatly simplifies the device fabrication process, especially the access to truly 3D geometries, is cost-effective, and it enables multimaterial processing. In this review, we discuss both the basics and recent innovations in PµSL; the material basis with emphasis on custom-made photopolymer formulations; multimaterial 3D printing; and, 3D-printed microfluidic devices for emulsion formation as our focus application. Our goal is to support researchers in setting up their own PµSL system to fabricate tailor-made microfluidics.
7
Soum, Veasna, Sooyong Park, Albertus Ivan Brilian, Oh-Sun Kwon i Kwanwoo Shin. "Programmable Paper-Based Microfluidic Devices for Biomarker Detections". Micromachines 10, nr 8 (2.08.2019): 516. http://dx.doi.org/10.3390/mi10080516.
Pełny tekst źródłaStreszczenie:
Recent advanced paper-based microfluidic devices provide an alternative technology for the detection of biomarkers by using affordable and portable devices for point-of-care testing (POCT). Programmable paper-based microfluidic devices enable a wide range of biomarker detection with high sensitivity and automation for single- and multi-step assays because they provide better control for manipulating fluid samples. In this review, we examine the advances in programmable microfluidics, i.e., paper-based continuous-flow microfluidic (p-CMF) devices and paper-based digital microfluidic (p-DMF) devices, for biomarker detection. First, we discuss the methods used to fabricate these two types of paper-based microfluidic devices and the strategies for programming fluid delivery and for droplet manipulation. Next, we discuss the use of these programmable paper-based devices for the single- and multi-step detection of biomarkers. Finally, we present the current limitations of paper-based microfluidics for biomarker detection and the outlook for their development.
8
Yap, Boon, Siti M.Soair, Noor Talik, Wai Lim i Lai Mei I. "Potential Point-of-Care Microfluidic Devices to Diagnose Iron Deficiency Anemia". Sensors 18, nr 8 (10.08.2018): 2625. http://dx.doi.org/10.3390/s18082625.
Pełny tekst źródłaStreszczenie:
Over the past 20 years, rapid technological advancement in the field of microfluidics has produced a wide array of microfluidic point-of-care (POC) diagnostic devices for the healthcare industry. However, potential microfluidic applications in the field of nutrition, specifically to diagnose iron deficiency anemia (IDA) detection, remain scarce. Iron deficiency anemia is the most common form of anemia, which affects billions of people globally, especially the elderly, women, and children. This review comprehensively analyzes the current diagnosis technologies that address anemia-related IDA-POC microfluidic devices in the future. This review briefly highlights various microfluidics devices that have the potential to detect IDA and discusses some commercially available devices for blood plasma separation mechanisms. Reagent deposition and integration into microfluidic devices are also explored. Finally, we discuss the challenges of insights into potential portable microfluidic systems, especially for remote IDA detection.
9
Chen, Luyao, Xin Guo, Xidi Sun, Shuming Zhang, Jing Wu, Huiwen Yu, Tongju Zhang, Wen Cheng, Yi Shi i Lijia Pan. "Porous Structural Microfluidic Device for Biomedical Diagnosis: A Review". Micromachines 14, nr 3 (26.02.2023): 547. http://dx.doi.org/10.3390/mi14030547.
Pełny tekst źródłaStreszczenie:
Microfluidics has recently received more and more attention in applications such as biomedical, chemical and medicine. With the development of microelectronics technology as well as material science in recent years, microfluidic devices have made great progress. Porous structures as a discontinuous medium in which the special flow phenomena of fluids lead to their potential and special applications in microfluidics offer a unique way to develop completely new microfluidic chips. In this article, we firstly introduce the fabrication methods for porous structures of different materials. Then, the physical effects of microfluid flow in porous media and their related physical models are discussed. Finally, the state-of-the-art porous microfluidic chips and their applications in biomedicine are summarized, and we present the current problems and future directions in this field.
10
Chen, Pin Chuan, i Zhi Ping Wang. "A Rapid and Low Cost Manufacturing for Polymeric Microfluidic Devices". Advanced Materials Research 579 (październik 2012): 348–56. http://dx.doi.org/10.4028/www.scientific.net/amr.579.348.
Pełny tekst źródłaStreszczenie:
A rapid manufacturing process was demonstrated to fabricate a microfluidic device to amplify specific DNA fragments in less than 8 hours. Microfluidics was derived from microelectromechanical system (MEMS) with lithography technique on the substrates of silicon and glass, which made the microfluidic product have a higher fabrication cost and laborious fabrication steps. This rapid approach only requires three steps for a PDMS microfluidic device: metal mold insert manufacturing, PDMS casting, and glass bonding. Each step did not require complicated equipments or procedures, and make this approach very attractive in rapid prototyping and experimental optimization with microfluidic devices. In this work, a brass mold insert was manufactured by a micromilling machine, followed by the standard PDMS casting and glass bonding to fabricate a microfluidic device. Polymerase chain reaction (PCR) to amplify specific DNA fragments, a typical microfluidic example, was successfully realized on this PDMS microfluidic device. This rapid and low cost (compared to conventional lithography) fabrication approach can provide researchers a lower entry to polymeric lab-on-a-chip either on PDMS or thermoplastic substrate for various applications.
11
Min, Lingli, Songyue Chen, Xinwen Xie, Hepeng Dong, Hong Pan, Zhizhi Sheng, Honglong Wang, Feng Wu, Miao Wang i Xu Hou. "Development and application of bio-inspired microfluidics". International Journal of Modern Physics B 32, nr 18 (15.07.2018): 1840013. http://dx.doi.org/10.1142/s0217979218400131.
Pełny tekst źródłaStreszczenie:
Bio-inspired microfluidic systems can be obtained through multidisciplinary approaches by using bio-inspired structural and functional designs for the microfluidic devices. This review mainly focuses on the concept of bio-inspired microfluidics to improve the properties of microfluidic systems for breaking through the bottlenecks of the current microfluidic devices, such as anti-fouling, smart, and dynamic response inside the microchannels under different environments. In addition, here, we show the current research progress of bio-inspired microfluidic systems in applications related to anti-fouling and smart devices, and biomedical research. The review discusses both physical theories and critical technologies in the bio-inspired microfluidics, from biomimetic design to real-world applications, so as to offer new ideas for the design and application of smart microfluidics, and the authors hope this review will inspire the active interest of many scientists in the area of the development and application of soft matter, and multifunctional and smart bio-inspired devices.
12
Gharib, Ghazaleh, İsmail Bütün, Zülâl Muganlı, Gül Kozalak, İlayda Namlı, Seyedali Seyedmirzaei Sarraf, Vahid Ebrahimpour Ahmadi, Erçil Toyran, Andre J. van Wijnen i Ali Koşar. "Biomedical Applications of Microfluidic Devices: A Review". Biosensors 12, nr 11 (16.11.2022): 1023. http://dx.doi.org/10.3390/bios12111023.
Pełny tekst źródłaStreszczenie:
Both passive and active microfluidic chips are used in many biomedical and chemical applications to support fluid mixing, particle manipulations, and signal detection. Passive microfluidic devices are geometry-dependent, and their uses are rather limited. Active microfluidic devices include sensors or detectors that transduce chemical, biological, and physical changes into electrical or optical signals. Also, they are transduction devices that detect biological and chemical changes in biomedical applications, and they are highly versatile microfluidic tools for disease diagnosis and organ modeling. This review provides a comprehensive overview of the significant advances that have been made in the development of microfluidics devices. We will discuss the function of microfluidic devices as micromixers or as sorters of cells and substances (e.g., microfiltration, flow or displacement, and trapping). Microfluidic devices are fabricated using a range of techniques, including molding, etching, three-dimensional printing, and nanofabrication. Their broad utility lies in the detection of diagnostic biomarkers and organ-on-chip approaches that permit disease modeling in cancer, as well as uses in neurological, cardiovascular, hepatic, and pulmonary diseases. Biosensor applications allow for point-of-care testing, using assays based on enzymes, nanozymes, antibodies, or nucleic acids (DNA or RNA). An anticipated development in the field includes the optimization of techniques for the fabrication of microfluidic devices using biocompatible materials. These developments will increase biomedical versatility, reduce diagnostic costs, and accelerate diagnosis time of microfluidics technology.
13
Kim, Hojin, Alexander Zhbanov i Sung Yang. "Microfluidic Systems for Blood and Blood Cell Characterization". Biosensors 13, nr 1 (22.12.2022): 13. http://dx.doi.org/10.3390/bios13010013.
Pełny tekst źródłaStreszczenie:
A laboratory blood test is vital for assessing a patient’s health and disease status. Advances in microfluidic technology have opened the door for on-chip blood analysis. Currently, microfluidic devices can reproduce myriad routine laboratory blood tests. Considerable progress has been made in microfluidic cytometry, blood cell separation, and characterization. Along with the usual clinical parameters, microfluidics makes it possible to determine the physical properties of blood and blood cells. We review recent advances in microfluidic systems for measuring the physical properties and biophysical characteristics of blood and blood cells. Added emphasis is placed on multifunctional platforms that combine several microfluidic technologies for effective cell characterization. The combination of hydrodynamic, optical, electromagnetic, and/or acoustic methods in a microfluidic device facilitates the precise determination of various physical properties of blood and blood cells. We analyzed the physical quantities that are measured by microfluidic devices and the parameters that are determined through these measurements. We discuss unexplored problems and present our perspectives on the long-term challenges and trends associated with the application of microfluidics in clinical laboratories. We expect the characterization of the physical properties of blood and blood cells in a microfluidic environment to be considered a standard blood test in the future.
14
Wu, Shigang, Xin Wang, Zongwen Li, Shijie Zhang i Fei Xing. "Recent Advances in the Fabrication and Application of Graphene Microfluidic Sensors". Micromachines 11, nr 12 (30.11.2020): 1059. http://dx.doi.org/10.3390/mi11121059.
Pełny tekst źródłaStreszczenie:
This review reports the progress of the recent development of graphene-based microfluidic sensors. The introduction of microfluidics technology provides an important possibility for the advance of graphene biosensor devices for a broad series of applications including clinical diagnosis, biological detection, health, and environment monitoring. Compared with traditional (optical, electrochemical, and biological) sensing systems, the combination of graphene and microfluidics produces many advantages, such as achieving miniaturization, decreasing the response time and consumption of chemicals, improving the reproducibility and sensitivity of devices. This article reviews the latest research progress of graphene microfluidic sensors in the fields of electrochemistry, optics, and biology. Here, the latest development trends of graphene-based microfluidic sensors as a new generation of detection tools in material preparation, device assembly, and chip materials are summarized. Special emphasis is placed on the working principles and applications of graphene-based microfluidic biosensors, especially in the detection of nucleic acid molecules, protein molecules, and bacterial cells. This article also discusses the challenges and prospects of graphene microfluidic biosensors.
15
Bogseth, Amanda, Jian Zhou i Ian Papautsky. "Evaluation of Performance and Tunability of a Co-Flow Inertial Microfluidic Device". Micromachines 11, nr 3 (10.03.2020): 287. http://dx.doi.org/10.3390/mi11030287.
Pełny tekst źródłaStreszczenie:
Microfluidics has gained a lot of attention for biological sample separation and purification methods over recent years. From many active and passive microfluidic techniques, inertial microfluidics offers a simple and efficient method to demonstrate various biological applications. One prevalent limitation of this method is its lack of tunability for different applications once the microfluidic devices are fabricated. In this work, we develop and characterize a co-flow inertial microfluidic device that is tunable in multiple ways for adaptation to different application requirements. In particular, flow rate, flow rate ratio and output resistance ratio are systematically evaluated for flexibility of the cutoff size of the device and modification of the separation performance post-fabrication. Typically, a mixture of single size particles is used to determine cutoff sizes for the outlets, yet this fails to provide accurate prediction for efficiency and purity for a more complex biological sample. Thus, we use particles with continuous size distribution (2–32 μm) for separation demonstration under conditions of various flow rates, flow rate ratios and resistance ratios. We also use A549 cancer cell line with continuous size distribution (12–27 μm) as an added demonstration. Our results indicate inertial microfluidic devices possess the tunability that offers multiple ways to improve device performance for adaptation to different applications even after the devices are prototyped.
16
Alhalaili, Badriyah, Ileana Nicoleta Popescu, Carmen Otilia Rusanescu i Ruxandra Vidu. "Microfluidic Devices and Microfluidics-Integrated Electrochemical and Optical (Bio)Sensors for Pollution Analysis: A Review". Sustainability 14, nr 19 (9.10.2022): 12844. http://dx.doi.org/10.3390/su141912844.
Pełny tekst źródłaStreszczenie:
An overview of the recent research works and trends in the design and fabrication of microfluidic devices and microfluidics-integrated biosensors for pollution analysis and monitoring of environmental contaminants is presented in this paper. In alignment with the tendency in miniaturization and integration into “lab on a chip” devices to reduce the use of reagents, energy, and implicit processing costs, the most common and newest materials used in the fabrication of microfluidic devices and microfluidics-integrated sensors and biosensors, the advantages and disadvantages of materials, fabrication methods, and the detection methods used for microfluidic environmental analysis are synthesized and evaluated.
17
Li, Qi, Xingchen Zhou, Qian Wang, Wenfang Liu i Chuanpin Chen. "Microfluidics for COVID-19: From Current Work to Future Perspective". Biosensors 13, nr 2 (20.01.2023): 163. http://dx.doi.org/10.3390/bios13020163.
Pełny tekst źródłaStreszczenie:
Spread of coronavirus disease 2019 (COVID-19) has significantly impacted the public health and economic sectors. It is urgently necessary to develop rapid, convenient, and cost-effective point-of-care testing (POCT) technologies for the early diagnosis and control of the plague’s transmission. Developing POCT methods and related devices is critical for achieving point-of-care diagnosis. With the advantages of miniaturization, high throughput, small sample requirements, and low actual consumption, microfluidics is an essential technology for the development of POCT devices. In this review, according to the different driving forces of the fluid, we introduce the common POCT devices based on microfluidic technology on the market, including paper-based microfluidic, centrifugal microfluidic, optical fluid, and digital microfluidic platforms. Furthermore, various microfluidic-based assays for diagnosing COVID-19 are summarized, including immunoassays, such as ELISA, and molecular assays, such as PCR. Finally, the challenges of and future perspectives on microfluidic device design and development are presented. The ultimate goals of this paper are to provide new insights and directions for the development of microfluidic diagnostics while expecting to contribute to the control of COVID-19.
18
Giri, Kiran, i Chia-Wen Tsao. "Recent Advances in Thermoplastic Microfluidic Bonding". Micromachines 13, nr 3 (20.03.2022): 486. http://dx.doi.org/10.3390/mi13030486.
Pełny tekst źródłaStreszczenie:
Microfluidics is a multidisciplinary technology with applications in various fields, such as biomedical, energy, chemicals and environment. Thermoplastic is one of the most prominent materials for polymer microfluidics. Properties such as good mechanical rigidity, organic solvent resistivity, acid/base resistivity, and low water absorbance make thermoplastics suitable for various microfluidic applications. However, bonding of thermoplastics has always been challenging because of a wide range of bonding methods and requirements. This review paper summarizes the current bonding processes being practiced for the fabrication of thermoplastic microfluidic devices, and provides a comparison between the different bonding strategies to assist researchers in finding appropriate bonding methods for microfluidic device assembly.
19
Damiati, Laila A., Marwa El-Yaagoubi, Safa A. Damiati, Rimantas Kodzius, Farshid Sefat i Samar Damiati. "Role of Polymers in Microfluidic Devices". Polymers 14, nr 23 (25.11.2022): 5132. http://dx.doi.org/10.3390/polym14235132.
Pełny tekst źródłaStreszczenie:
Polymers are sustainable and renewable materials that are in high demand due to their excellent properties. Natural and synthetic polymers with high flexibility, good biocompatibility, good degradation rate, and stiffness are widely used for various applications, such as tissue engineering, drug delivery, and microfluidic chip fabrication. Indeed, recent advances in microfluidic technology allow the fabrication of polymeric matrix to construct microfluidic scaffolds for tissue engineering and to set up a well-controlled microenvironment for manipulating fluids and particles. In this review, polymers as materials for the fabrication of microfluidic chips have been highlighted. Successful models exploiting polymers in microfluidic devices to generate uniform particles as drug vehicles or artificial cells have been also discussed. Additionally, using polymers as bioink for 3D printing or as a matrix to functionalize the sensing surface in microfluidic devices has also been mentioned. The rapid progress made in the combination of polymers and microfluidics presents a low-cost, reproducible, and scalable approach for a promising future in the manufacturing of biomimetic scaffolds for tissue engineering.
20
Switalla, Ander, Lael Wentland i Elain Fu. "3D printing-based microfluidic devices in fabric". Journal of Micromechanics and Microengineering 33, nr 2 (19.01.2023): 027001. http://dx.doi.org/10.1088/1361-6439/acaff1.
Pełny tekst źródłaStreszczenie:
Abstract Fabric-based microfluidics is a growing sub-field of porous materials-based microfluidics. 3D printing has been demonstrated as a useful fabrication method for open channel microfluidic devices, and also in the context of porous substates such as cellulose. In the current report, we describe a straightforward method for 3D printing fabric-based microfluidic devices. We demonstrate the ability to create both full and partial barriers in fabric, characterizing minimum channel and barrier widths, as well as reproducibility of the method using the metric of flow time repeatability through the channels. We discuss considerations specific to 3D printing in fabric including fabric anisotropy, stretching, and nonuniformity. Further, we highlight our fabrication method via the implementation of a colorimetric urea assay.
21
James, Matthew, Richard A. Revia, Zachary Stephen i Miqin Zhang. "Microfluidic Synthesis of Iron Oxide Nanoparticles". Nanomaterials 10, nr 11 (23.10.2020): 2113. http://dx.doi.org/10.3390/nano10112113.
Pełny tekst źródłaStreszczenie:
Research efforts into the production and application of iron oxide nanoparticles (IONPs) in recent decades have shown IONPs to be promising for a range of biomedical applications. Many synthesis techniques have been developed to produce high-quality IONPs that are safe for in vivo environments while also being able to perform useful biological functions. Among them, coprecipitation is the most commonly used method but has several limitations such as polydisperse IONPs, long synthesis times, and batch-to-batch variations. Recent efforts at addressing these limitations have led to the development of microfluidic devices that can make IONPs of much-improved quality. Here, we review recent advances in the development of microfluidic devices for the synthesis of IONPs by coprecipitation. We discuss the main architectures used in microfluidic device design and highlight the most prominent manufacturing methods and materials used to construct these microfluidic devices. Finally, we discuss the benefits that microfluidics can offer to the coprecipitation synthesis process including the ability to better control various synthesis parameters and produce IONPs with high production rates.
22
Zhang, Peiran, Hunter Bachman, Adem Ozcelik i Tony Jun Huang. "Acoustic Microfluidics". Annual Review of Analytical Chemistry 13, nr 1 (12.06.2020): 17–43. http://dx.doi.org/10.1146/annurev-anchem-090919-102205.
Pełny tekst źródłaStreszczenie:
Acoustic microfluidic devices are powerful tools that use sound waves to manipulate micro- or nanoscale objects or fluids in analytical chemistry and biomedicine. Their simple device designs, biocompatible and contactless operation, and label-free nature are all characteristics that make acoustic microfluidic devices ideal platforms for fundamental research, diagnostics, and therapeutics. Herein, we summarize the physical principles underlying acoustic microfluidics and review their applications, with particular emphasis on the manipulation of macromolecules, cells, particles, model organisms, and fluidic flows. We also present future goals of this technology in analytical chemistry and biomedical research, as well as challenges and opportunities.
23
Marques, Marco PC, i Nicolas Szita. "Bioprocess microfluidics: applying microfluidic devices for bioprocessing". Current Opinion in Chemical Engineering 18 (listopad 2017): 61–68. http://dx.doi.org/10.1016/j.coche.2017.09.004.
Pełny tekst źródła
24
Xi, Wang, Fang Kong, Joo Chuan Yeo, Longteng Yu, Surabhi Sonam, Ming Dao, Xiaobo Gong i Chwee Teck Lim. "Soft tubular microfluidics for 2D and 3D applications". Proceedings of the National Academy of Sciences 114, nr 40 (18.09.2017): 10590–95. http://dx.doi.org/10.1073/pnas.1712195114.
Pełny tekst źródłaStreszczenie:
Microfluidics has been the key component for many applications, including biomedical devices, chemical processors, microactuators, and even wearable devices. This technology relies on soft lithography fabrication which requires cleanroom facilities. Although popular, this method is expensive and labor-intensive. Furthermore, current conventional microfluidic chips precludes reconfiguration, making reiterations in design very time-consuming and costly. To address these intrinsic drawbacks of microfabrication, we present an alternative solution for the rapid prototyping of microfluidic elements such as microtubes, valves, and pumps. In addition, we demonstrate how microtubes with channels of various lengths and cross-sections can be attached modularly into 2D and 3D microfluidic systems for functional applications. We introduce a facile method of fabricating elastomeric microtubes as the basic building blocks for microfluidic devices. These microtubes are transparent, biocompatible, highly deformable, and customizable to various sizes and cross-sectional geometries. By configuring the microtubes into deterministic geometry, we enable rapid, low-cost formation of microfluidic assemblies without compromising their precision and functionality. We demonstrate configurable 2D and 3D microfluidic systems for applications in different domains. These include microparticle sorting, microdroplet generation, biocatalytic micromotor, triboelectric sensor, and even wearable sensing. Our approach, termed soft tubular microfluidics, provides a simple, cheaper, and faster solution for users lacking proficiency and access to cleanroom facilities to design and rapidly construct microfluidic devices for their various applications and needs.
25
Kong, David S., Todd A. Thorsen, Jonathan Babb, Scott T. Wick, Jeremy J. Gam, Ron Weiss i Peter A. Carr. "Open-source, community-driven microfluidics with Metafluidics". Nature Biotechnology 35, nr 6 (czerwiec 2017): 523–29. http://dx.doi.org/10.1038/nbt.3873.
Pełny tekst źródłaStreszczenie:
Abstract Microfluidic devices have the potential to automate and miniaturize biological experiments, but open-source sharing of device designs has lagged behind sharing of other resources such as software. Synthetic biologists have used microfluidics for DNA assembly, cell-free expression, and cell culture, but a combination of expense, device complexity, and reliance on custom set-ups hampers their widespread adoption. We present Metafluidics, an open-source, community-driven repository that hosts digital design files, assembly specifications, and open-source software to enable users to build, configure, and operate a microfluidic device. We use Metafluidics to share designs and fabrication instructions for both a microfluidic ring-mixer device and a 32-channel tabletop microfluidic controller. This device and controller are applied to build genetic circuits using standard DNA assembly methods including ligation, Gateway, Gibson, and Golden Gate. Metafluidics is intended to enable a broad community of engineers, DIY enthusiasts, and other nontraditional participants with limited fabrication skills to contribute to microfluidic research.
26
Duan, Kai, Mohamad Orabi, Alexus Warchock, Zaynab Al-Akraa, Zeinab Ajami, Tae-Hwa Chun i Joe F. Lo. "Monolithically 3D-Printed Microfluidics with Embedded µTesla Pump". Micromachines 14, nr 2 (17.01.2023): 237. http://dx.doi.org/10.3390/mi14020237.
Pełny tekst źródłaStreszczenie:
Microfluidics has earned a reputation for providing numerous transformative but disconnected devices and techniques. Active research seeks to address this challenge by integrating microfluidic components, including embedded miniature pumps. However, a significant portion of existing microfluidic integration relies on the time-consuming manual fabrication that introduces device variations. We put forward a framework for solving this disconnect by combining new pumping mechanics and 3D printing to demonstrate several novel, integrated and wirelessly driven microfluidics. First, we characterized the simplicity and performance of printed microfluidics with a minimum feature size of 100 µm. Next, we integrated a microtesla (µTesla) pump to provide non-pulsatile flow with reduced shear stress on beta cells cultured on-chip. Lastly, the integration of radio frequency (RF) device and a hobby-grade brushless motor completed a self-enclosed platform that can be remotely controlled without wires. Our study shows how new physics and 3D printing approaches not only provide better integration but also enable novel cell-based studies to advance microfluidic research.
27
Trinh, Kieu The Loan, Duc Anh Thai i Nae Yoon Lee. "Bonding Strategies for Thermoplastics Applicable for Bioanalysis and Diagnostics". Micromachines 13, nr 9 (10.09.2022): 1503. http://dx.doi.org/10.3390/mi13091503.
Pełny tekst źródłaStreszczenie:
Microfluidics is a multidisciplinary science that includes physics, chemistry, engineering, and biotechnology. Such microscale systems are receiving growing interest in applications such as analysis, diagnostics, and biomedical research. Thermoplastic polymers have emerged as one of the most attractive materials for microfluidic device fabrication owing to advantages such as being optically transparent, biocompatible, cost-effective, and mass producible. However, thermoplastic bonding is a key challenge for sealing microfluidic devices. Given the wide range of bonding methods, the appropriate bonding approach should be carefully selected depending on the thermoplastic material and functional requirements. In this review, we aim to provide a comprehensive overview of thermoplastic fabricating and bonding approaches, presenting their advantages and disadvantages, to assist in finding suitable microfluidic device bonding methods. In addition, we highlight current applications of thermoplastic microfluidics to analyses and diagnostics and introduce future perspectives on thermoplastic bonding strategies.
28
Hammami, Saber, Aleksandr Oseev, Sylwester Bargiel, Rabah Zeggari, Céline Elie-Caille i Thérèse Leblois. "Microfluidics for High Pressure: Integration on GaAs Acoustic Biosensors with a Leakage-Free PDMS Based on Bonding Technology". Micromachines 13, nr 5 (11.05.2022): 755. http://dx.doi.org/10.3390/mi13050755.
Pełny tekst źródłaStreszczenie:
Microfluidics integration of acoustic biosensors is an actively developing field. Despite significant progress in “passive” microfluidic technology, integration with microacoustic devices is still in its research state. The major challenge is bonding polymers with monocrystalline piezoelectrics to seal microfluidic biosensors. In this contribution, we specifically address the challenge of microfluidics integration on gallium arsenide (GaAs) acoustic biosensors. We have developed a robust plasma-assisted bonding technology, allowing strong connections between PDMS microfluidic chip and GaAs/SiO2 at low temperatures (70 °C). Mechanical and fluidic performances of fabricated device were studied. The bonding surfaces were characterized by water contact angle measurement and ATR-FTIR, AFM, and SEM analysis. The bonding strength was characterized using a tensile machine and pressure/leakage tests. The study showed that the sealed chips were able to achieve a limit of high bonding strength of 2.01 MPa. The adhesion of PDMS to GaAs was significantly improved by use of SiO2 intermediate layer, permitting the bonded chip to withstand at least 8.5 bar of burst pressure. The developed bonding approach can be a valuable solution for microfluidics integration in several types of MEMS devices.
29
Subirada, Francesc, Roberto Paoli, Jessica Sierra-Agudelo, Anna Lagunas, Romen Rodriguez-Trujillo i Josep Samitier. "Development of a Custom-Made 3D Printing Protocol with Commercial Resins for Manufacturing Microfluidic Devices". Polymers 14, nr 14 (21.07.2022): 2955. http://dx.doi.org/10.3390/polym14142955.
Pełny tekst źródłaStreszczenie:
The combination of microfluidics and photo-polymerization techniques such as stereolithography (SLA) has emerged as a new field which has a lot of potential to influence in such important areas as biological analysis, and chemical detection among others. However, the integration between them is still at an early stage of development. In this article, after analyzing the resolution of a custom SLA 3D printer with commercial resins, microfluidic devices were manufactured using three different approaches. First, printing a mold with the objective of creating a Polydimethylsiloxane (PDMS) replica with the microfluidic channels; secondly, open channels have been printed and then assembled with a flat cover of the same resin material. Finally, a closed microfluidic device has also been produced in a single process of printing. Important results for 3D printing with commercial resins have been achieved by only printing one layer on top of the channel. All microfluidic devices have been tested successfully for pressure-driven fluid flow.
30
Naderi, Arman, Nirveek Bhattacharjee i Albert Folch. "Digital Manufacturing for Microfluidics". Annual Review of Biomedical Engineering 21, nr 1 (4.06.2019): 325–64. http://dx.doi.org/10.1146/annurev-bioeng-092618-020341.
Pełny tekst źródłaStreszczenie:
The microfluidics field is at a critical crossroads. The vast majority of microfluidic devices are presently manufactured using micromolding processes that work very well for a reduced set of biocompatible materials, but the time, cost, and design constraints of micromolding hinder the commercialization of many devices. As a result, the dissemination of microfluidic technology—and its impact on society—is in jeopardy. Digital manufacturing (DM) refers to a family of computer-centered processes that integrate digital three-dimensional (3D) designs, automated (additive or subtractive) fabrication, and device testing in order to increase fabrication efficiency. Importantly, DM enables the inexpensive realization of 3D designs that are impossible or very difficult to mold. The adoption of DM by microfluidic engineers has been slow, likely due to concerns over the resolution of the printers and the biocompatibility of the resins. In this article, we review and discuss the various printer types, resolution, biocompatibility issues, DM microfluidic designs, and the bright future ahead for this promising, fertile field.
31
Tonooka, Taishi. "Microfluidic Device with an Integrated Freeze-Dried Cell-Free Protein Synthesis System for Small-Volume Biosensing". Micromachines 12, nr 1 (29.12.2020): 27. http://dx.doi.org/10.3390/mi12010027.
Pełny tekst źródłaStreszczenie:
Microfluidic devices enable the precise operation of liquid samples in small volumes. This motivates why microfluidic devices have been applied to point-of-care (PoC) liquid biopsy. Among PoC liquid biopsy studies, some report diagnostic reagents being freeze-dried in such microfluidic devices. This type of PoC microfluidic device has distinct advantages, such as simplicity of the procedures, compared with other PoC devices using liquid-type diagnostic reagents. Despite the attractive characteristic, only diagnostic reagents based on the cloned enzyme donor immunoassay (CEDIA) have been freeze-dried in the microfluidic device. However, development of the PoC device based on the CEDIA method is time-consuming and labor-intensive. Here, we employed a molecule-responsive protein synthesis system as the diagnostic reagent to be freeze-dried in the microfluidic device. Such molecule-responsive protein synthesis has been well investigated in the field of molecular biology. Therefore, using the accumulated information, PoC devices can be efficiently developed. Thus, we developed a microfluidic device with an integrated freeze-dried molecule-responsive protein synthesis system. Using the developed device, we detected two types of bio-functional molecules (i.e., bacterial quorum sensing molecules and mercury ions) by injecting 1 µL of sample solution containing these molecules. We showed that the developed device is applicable for small-volume biosensing.
32
Man, Jia, Luming Man, Chenchen Zhou, Jianyong Li, Shuaishuai Liang, Song Zhang i Jianfeng Li. "A Facile Single-Phase-Fluid-Driven Bubble Microfluidic Generator for Potential Detection of Viruses Suspended in Air". Biosensors 12, nr 5 (3.05.2022): 294. http://dx.doi.org/10.3390/bios12050294.
Pełny tekst źródłaStreszczenie:
Microfluidics devices have widely been employed to prepare monodispersed microbubbles/droplets, which have promising applications in biomedical engineering, biosensor detection, drug delivery, etc. However, the current reported microfluidic devices need to control at least two-phase fluids to make microbubbles/droplets. Additionally, it seems to be difficult to make monodispersed microbubbles from the ambient air using currently reported microfluidic structures. Here, we present a facile approach to making monodispersed microbubbles directly from the ambient air by driving single-phase fluid. The reported single-phase-fluid microfluidic (SPFM) device has a typical co-flow structure, while the adjacent space between the injection tube and the collection tube is open to the air. The flow condition inside the SPFM device was systematically studied. By adjusting the flow rate of the single-phase fluid, bubbles were generated, the sizes of which could be tuned precisely. This facile bubble generator may have significant potential as a detection sensor in detecting viruses in spread droplets or haze particles in ambient air.
33
Gorgannezhad, Lena, Helen Stratton i Nam-Trung Nguyen. "Microfluidic-Based Nucleic Acid Amplification Systems in Microbiology". Micromachines 10, nr 6 (19.06.2019): 408. http://dx.doi.org/10.3390/mi10060408.
Pełny tekst źródłaStreszczenie:
Rapid, sensitive, and selective bacterial detection is a hot topic, because the progress in this research area has had a broad range of applications. Novel and innovative strategies for detection and identification of bacterial nucleic acids are important for practical applications. Microfluidics is an emerging technology that only requires small amounts of liquid samples. Microfluidic devices allow for rapid advances in microbiology, enabling access to methods of amplifying nucleic acid molecules and overcoming difficulties faced by conventional. In this review, we summarize the recent progress in microfluidics-based polymerase chain reaction devices for the detection of nucleic acid biomarkers. The paper also discusses the recent development of isothermal nucleic acid amplification and droplet-based microfluidics devices. We discuss recent microfluidic techniques for sample preparation prior to the amplification process.
34
Catarino, Susana O., Raquel O. Rodrigues, Diana Pinho, João M. Miranda, Graça Minas i Rui Lima. "Blood Cells Separation and Sorting Techniques of Passive Microfluidic Devices: From Fabrication to Applications". Micromachines 10, nr 9 (10.09.2019): 593. http://dx.doi.org/10.3390/mi10090593.
Pełny tekst źródłaStreszczenie:
Since the first microfluidic device was developed more than three decades ago, microfluidics is seen as a technology that exhibits unique features to provide a significant change in the way that modern biology is performed. Blood and blood cells are recognized as important biomarkers of many diseases. Taken advantage of microfluidics assets, changes on blood cell physicochemical properties can be used for fast and accurate clinical diagnosis. In this review, an overview of the microfabrication techniques is given, especially for biomedical applications, as well as a synopsis of some design considerations regarding microfluidic devices. The blood cells separation and sorting techniques were also reviewed, highlighting the main achievements and breakthroughs in the last decades.
35
Wang, Xu, Jingtian Zheng, Maheshwar Adiraj Iyer, Adam Henry Szmelter, David T. Eddington i Steve Seung-Young Lee. "Spatially selective cell treatment and collection for integrative drug testing using hydrodynamic flow focusing and shifting". PLOS ONE 18, nr 1 (17.01.2023): e0279102. http://dx.doi.org/10.1371/journal.pone.0279102.
Pełny tekst źródłaStreszczenie:
Hydrodynamic focusing capable of readily producing and controlling laminar flow facilitates drug treatment of cells in existing microfluidic culture devices. However, to expand applications of such devices to multiparameter drug testing, critical limitations in current hydrodynamic focusing microfluidics must be addressed. Here we describe hydrodynamic focusing and shifting as an advanced microfluidics tool for spatially selective drug delivery and integrative cell-based drug testing. We designed and fabricated a co-flow focusing, three-channel microfluidic device with a wide cell culture chamber. By controlling inlet flow rates of sample and two side solutions, we could generate hydrodynamic focusing and shifting that mediated precise regulation of the path and width of reagent and drug stream in the microfluidic device. We successfully validated a hydrodynamic focusing and shifting approach for spatially selective delivery of DiI, a lipophilic fluorophore, and doxorubicin, a chemotherapeutic agent, to tumor cells in our device. Moreover, subsequent flowing of a trypsin EDTA solution over the cells that were exposed to doxorubicin flow allowed us to selectively collect the treated cells. Our approach enabled downstream high-resolution microscopy of the cell suspension to confirm the nuclear delivery of doxorubicin into the tumor cells. In the device, we could also evaluate in situ the cytotoxic effect of doxorubicin to the tumor cells that were selectively treated by hydrodynamic flow focusing and shifting. These results show that hydrodynamic focusing and shifting enable a fast and robust approach to spatially treat and then collect cells in an optimized microfluidic device, offering an integrative assay tool for efficient drug screening and discovery.
36
Oh, Kwang W. "Microfluidic Devices for Biomedical Applications: Biomedical Microfluidic Devices 2019". Micromachines 11, nr 4 (1.04.2020): 370. http://dx.doi.org/10.3390/mi11040370.
Pełny tekst źródła
37
Yang, Shih-Mo, Shuangsong Lv, Wenjun Zhang i Yubao Cui. "Microfluidic Point-of-Care (POC) Devices in Early Diagnosis: A Review of Opportunities and Challenges". Sensors 22, nr 4 (18.02.2022): 1620. http://dx.doi.org/10.3390/s22041620.
Pełny tekst źródłaStreszczenie:
The early diagnosis of infectious diseases is critical because it can greatly increase recovery rates and prevent the spread of diseases such as COVID-19; however, in many areas with insufficient medical facilities, the timely detection of diseases is challenging. Conventional medical testing methods require specialized laboratory equipment and well-trained operators, limiting the applicability of these tests. Microfluidic point-of-care (POC) equipment can rapidly detect diseases at low cost. This technology could be used to detect diseases in underdeveloped areas to reduce the effects of disease and improve quality of life in these areas. This review details microfluidic POC equipment and its applications. First, the concept of microfluidic POC devices is discussed. We then describe applications of microfluidic POC devices for infectious diseases, cardiovascular diseases, tumors (cancer), and chronic diseases, and discuss the future incorporation of microfluidic POC devices into applications such as wearable devices and telemedicine. Finally, the review concludes by analyzing the present state of the microfluidic field, and suggestions are made. This review is intended to call attention to the status of disease treatment in underdeveloped areas and to encourage the researchers of microfluidics to develop standards for these devices.
38
Kaaliveetil, Sreerag, Juliana Yang, Saud Alssaidy, Zhenglong Li, Yu-Hsuan Cheng, Niranjan Haridas Menon, Charmi Chande i Sagnik Basuray. "Microfluidic Gas Sensors: Detection Principle and Applications". Micromachines 13, nr 10 (11.10.2022): 1716. http://dx.doi.org/10.3390/mi13101716.
Pełny tekst źródłaStreszczenie:
With the rapid growth of emerging point-of-use (POU)/point-of-care (POC) detection technologies, miniaturized sensors for the real-time detection of gases and airborne pathogens have become essential to fight pollution, emerging contaminants, and pandemics. However, the low-cost development of miniaturized gas sensors without compromising selectivity, sensitivity, and response time remains challenging. Microfluidics is a promising technology that has been exploited for decades to overcome such limitations, making it an excellent candidate for POU/POC. However, microfluidic-based gas sensors remain a nascent field. In this review, the evolution of microfluidic gas sensors from basic electronic techniques to more advanced optical techniques such as surface-enhanced Raman spectroscopy to detect analytes is documented in detail. This paper focuses on the various detection methodologies used in microfluidic-based devices for detecting gases and airborne pathogens. Non-continuous microfluidic devices such as bubble/droplet-based microfluidics technology that have been employed to detect gases and airborne pathogens are also discussed. The selectivity, sensitivity, advantages/disadvantages vis-a-vis response time, and fabrication costs for all the microfluidic sensors are tabulated. The microfluidic sensors are grouped based on the target moiety, such as air pollutants such as carbon monoxide and nitrogen oxides, and airborne pathogens such as E. coli and SARS-CoV-2. The possible application scenarios for the various microfluidic devices are critically examined.
39
Babikian, Sarkis, Brian Soriano, G. P. Li i Mark Bachman. "Laminate Materials for Microfluidic PCBs". International Symposium on Microelectronics 2012, nr 1 (1.01.2012): 000162–68. http://dx.doi.org/10.4071/isom-2012-ta54.
Pełny tekst źródłaStreszczenie:
The printed circuit board (PCB) is a very attractive platform to produce highly integrated highly functional microfluidic devices. We have investigated laminate materials and developed novel fabrication processes to realize low cost and scalable to manufacturing integrated microfluidics on PCBs. In this paper we describe our vision to integrate functional components with microfluidic channels. We also report on the use of Ethylene Vinyl Acetate (EVA) as a laminate for microfluidics. The material was characterized for microfluidic applications and compared with our previously reported laminates: 1002F and Polyurethane.
40
Deng, B., X. F. Li, D. Y. Chen, L. D. You, J. B. Wang i J. Chen. "Parameter Screening in Microfluidics Based Hydrodynamic Single-Cell Trapping". Scientific World Journal 2014 (2014): 1–8. http://dx.doi.org/10.1155/2014/929163.
Pełny tekst źródłaStreszczenie:
Microfluidic cell-based arraying technology is widely used in the field of single-cell analysis. However, among developed devices, there is a compromise between cellular loading efficiencies and trapped cell densities, which deserves further analysis and optimization. To address this issue, the cell trapping efficiency of a microfluidic device with two parallel micro channels interconnected with cellular trapping sites was studied in this paper. By regulating channel inlet and outlet status, the microfluidic trapping structure can mimic key functioning units of previously reported devices. Numerical simulations were used to model this cellular trapping structure, quantifying the effects of channel on/off status and trapping structure geometries on the cellular trapping efficiency. Furthermore, the microfluidic device was fabricated based on conventional microfabrication and the cellular trapping efficiency was quantified in experiments. Experimental results showed that, besides geometry parameters, cellular travelling velocities and sizes also affected the single-cell trapping efficiency. By fine tuning parameters, more than 95% of trapping sites were taken by individual cells. This study may lay foundation in further studies of single-cell positioning in microfluidics and push forward the study of single-cell analysis.
41
Ota, Nobutoshi, Yaxiaer Yalikun, Tomoyuki Suzuki, Sang Wook Lee, Yoichiroh Hosokawa, Keisuke Goda i Yo Tanaka. "Enhancement in acoustic focusing of micro and nanoparticles by thinning a microfluidic device". Royal Society Open Science 6, nr 2 (luty 2019): 181776. http://dx.doi.org/10.1098/rsos.181776.
Pełny tekst źródłaStreszczenie:
The manipulation of micro/nanoparticles has become increasingly important in biological and industrial fields. As a non-contact method for particle manipulation, acoustic focusing has been applied in sorting, enrichment and analysis of particles with microfluidic devices. Although the frequency and amplitude of acoustic waves and the dimensions of microchannels have been recognized as important parameters for acoustic focusing, the thickness of microfluidic devices has not been considered so far. Here, we report that thin glass microfluidic devices enhance acoustic focusing of micro/nanoparticles. It was found that the thickness of a microfluidic device strongly influences its ability to focus particles via acoustic radiation, because the energy propagation of acoustic waves is affected by the total mass of the device. Acoustic focusing of submicrometre polystyrene beads and Escherichia coli as well as enrichment of polystyrene beads were achieved in glass microfluidic devices as thin as 0.4 mm. Modifying the thickness of a microfluidic device can thus serve as a critical parameter for acoustic focusing when conventional parameters to achieve this effect are kept unchanged. Thus, our findings enable new approaches to the design of novel microfluidic devices.
42
Islam, Md Nazibul, Jarad Yost i Zachary Gagnon. "Electrokinetically Assisted Paper-Based DNA Concentration for Enhanced qPCR Sensing". Proceedings 60, nr 1 (2.11.2020): 33. http://dx.doi.org/10.3390/iecb2020-07074.
Pełny tekst źródłaStreszczenie:
Paper-based microfluidics have gained widespread attention for use as low-cost microfluidic diagnostic devices in low-resource settings. However, variability in fluid transport due to evaporation and lack of reproducibility with processing real-world samples limits their commercial potential and widespread adoption. We have developed a novel fabrication method to address these challenges. This approach, known as “Microfluidic Pressure in Paper” (μPiP), combines thin laminating polydimethylsiloxane (PDMS) membranes and precision laser-cut paper microfluidic structures to produce devices that are low-cost, scalable, and exhibit controllable and reproducible fluid flow dynamics similar to conventional microfluidic devices. We present a new μPiP DNA sample preparation and processing device that reduces the number of sample preparation steps and improves sensitivity of the quantitative polymerase chain reaction (qPCR) by electrophoretically separating and concentrating nucleic acids (NAs) continuously on paper. Our device was assembled using two different microfluidic paper channels: one with a larger pore (25 microns) size for bulk fluid transport and another with a smaller pore size (11 microns) for electrophoretic sample concentration. These two paper types were aligned and laminated within PDMS sheets, and integrated with adhesive copper tape electrodes. A solution containing a custom DNA sequence was introduced into the large pore size paper channel using a low-cost pressure system and a DC voltage was applied to the copper tape to electrophoretically deflect the solution containing NAs into the paper channel with the smaller pore size. Samples were collected from both DNA enriched and depleted channels and analyzed using qPCR. Our results demonstrate the ability to use these paper devices to process and concentrate nucleic acids. Our concentration device has the potential to reduce the number of sample preparation steps and to improve qPCR sensitivity, which has immediate applications in disease diagnostics, microbial contamination, and public health monitoring.
43
Hassan, Sammer-ul, Aamira Tariq, Zobia Noreen, Ahmed Donia, Syed Z. J. Zaidi, Habib Bokhari i Xunli Zhang. "Capillary-Driven Flow Microfluidics Combined with Smartphone Detection: An Emerging Tool for Point-of-Care Diagnostics". Diagnostics 10, nr 8 (22.07.2020): 509. http://dx.doi.org/10.3390/diagnostics10080509.
Pełny tekst źródłaStreszczenie:
Point-of-care (POC) or near-patient testing allows clinicians to accurately achieve real-time diagnostic results performed at or near to the patient site. The outlook of POC devices is to provide quicker analyses that can lead to well-informed clinical decisions and hence improve the health of patients at the point-of-need. Microfluidics plays an important role in the development of POC devices. However, requirements of handling expertise, pumping systems and complex fluidic controls make the technology unaffordable to the current healthcare systems in the world. In recent years, capillary-driven flow microfluidics has emerged as an attractive microfluidic-based technology to overcome these limitations by offering robust, cost-effective and simple-to-operate devices. The internal wall of the microchannels can be pre-coated with reagents, and by merely dipping the device into the patient sample, the sample can be loaded into the microchannel driven by capillary forces and can be detected via handheld or smartphone-based detectors. The capabilities of capillary-driven flow devices have not been fully exploited in developing POC diagnostics, especially for antimicrobial resistance studies in clinical settings. The purpose of this review is to open up this field of microfluidics to the ever-expanding microfluidic-based scientific community.
44
Torino, Stefania, Brunella Corrado, Mario Iodice i Giuseppe Coppola. "PDMS-Based Microfluidic Devices for Cell Culture". Inventions 3, nr 3 (6.09.2018): 65. http://dx.doi.org/10.3390/inventions3030065.
Pełny tekst źródłaStreszczenie:
Microfluidic technology has affirmed itself as a powerful tool in medical and biological research by offering the possibility of managing biological samples in tiny channels and chambers. Among the different applications, the use of microfluidics for cell cultures has attracted much interest from scientists worldwide. Traditional cell culture methods need high quantities of samples and reagents that are strongly reduced in miniaturized systems. In addition, the microenvironment is better controlled by scaling down. In this paper, we provide an overview of the aspects related to the design of a novel microfluidic culture chamber, the fabrication approach based on polydimethylsiloxane (PDMS) soft-lithography, and the most critical issues in shrinking the size of the system.
45
Sharma, Smriti, i Vinayak Bhatia. "Magnetic nanoparticles in microfluidics-based diagnostics: an appraisal". Nanomedicine 16, nr 15 (czerwiec 2021): 1329–42. http://dx.doi.org/10.2217/nnm-2021-0007.
Pełny tekst źródłaStreszczenie:
The use of magnetic nanoparticles (MNPs) in microfluidics based diagnostics is a classic case of micro-, nano- and bio-technology coming together to design extremely controllable, reproducible, and scalable nano and micro ‘ on-chip bio sensing systems.’ In this review, applications of MNPs in microfluidics ranging from molecular diagnostics and immunodiagnostics to clinical uses have been examined. In addition, microfluidic mixing and capture of analytes using MNPs, and MNPs as carriers in microfluidic devices has been investigated. Finally, the challenges and future directions of this upcoming field have been summarized. The use of MNP-based microfluidic devices, will help in developing decentralized or ‘ point of care’ testing globally, contributing to affordable healthcare, particularly, for middle- and low-income developing countries.
46
Amoyav, Benzion, Yoel Goldstein, Eliana Steinberg i Ofra Benny. "3D Printed Microfluidic Devices for Drug Release Assays". Pharmaceutics 13, nr 1 (23.12.2020): 13. http://dx.doi.org/10.3390/pharmaceutics13010013.
Pełny tekst źródłaStreszczenie:
Microfluidics research for various applications, including drug delivery, cell-based assays and biomedical research has grown exponentially. Despite this technology’s enormous potential, drawbacks include the need for multistep fabrication, typically with lithography. We present a one-step fabrication process of a microfluidic chip for drug dissolution assays based on a 3D printing technology. Doxorubicin porous and non-porous microspheres, with a mean diameter of 250µm, were fabricated using a conventional “batch” or microfluidic method, based on an optimized solid-in-oil-in-water protocol. Microspheres fabricated with microfluidics system exhibited higher encapsulation efficiency and drug content as compared with batch formulations. We determined drug release profiles of microspheres in varying pH conditions using two distinct dissolution devices that differed in their mechanical barrier structures. The release profile of the “V” shape barrier was similar to that of the dialysis sac test and differed from the “basket” barrier design. Importantly, a cytotoxicity test confirmed biocompatibility of the printed resin. Finally, the chip exhibited high durability and stability, enabling multiple recycling sessions. We show how the combination of microfluidics and 3D printing can reduce costs and time, providing an efficient platform for particle production while offering a feasible cost-effective alternative to clean-room facility polydimethylsiloxane-based chip microfabrication.
47
Khetan, E., A. J. Maki i M. B. Wheeler. "289 STEM CELL CULTURE AND DIFFERENTIATION IN MICROFLUIDIC DEVICES". Reproduction, Fertility and Development 25, nr 1 (2013): 292. http://dx.doi.org/10.1071/rdv25n1ab289.
Pełny tekst źródłaStreszczenie:
Advancements in micro and nanotechnology have allowed scientists a powerful platform to study biological systems. Microfluidics is one area of advancement with great promise. Microfluidics deals with the behaviour, specific control, and manipulation of microliter and nanoliter volumes of fluid. The small-scale design of these microfluidic devices permits laminar flow, characterised as parallel streams flowing without disruption between currents. With the introduction of micro-technology and microfluidic platforms for cell culture, stem cell research can be put into a new context. Inside microfluidics, microenvironments can be more precisely controlled and they provide a more in vivo-like environment for the cells to grow and hence can serve as a better way of culturing the cells. In the current study, we examined the influence of microfluidic devices on the development of stem cells. Adipose-derived stem cells (ADSC) were isolated from pigs and seeded in a microfluidic device to differentiate toward adipogenic, osteogenic, and chondrogenic lineages using specific differentiation-promoting media (Monaco et al. 2009 Open Tissue Eng. Regen. Med. J. 2, 20–33). Five thousand cells were seeded per channel at a density of 5 000 000 cells mL–1. The microchannel dimensions were 5 mm long, 1 mm wid, and 200 µm deep. Cells were maintained for 14 days and then stained with respective staining dyes: Oil Red O for adipogenesis, Alizarin Red for osteogenesis, and Toluidine Blue for chondrogenesis. Cells differentiated towards adipogenic lineage contained small lipid droplets, which stained red with Oil Red O stain; during osteogenic differentiation, the cells formed large nodules and stained positive for the presence of calcium; and the chondriogenic differentiating cells showed the presence of proteoglycans (blue) when stained with Toluidine Blue. We seeded ADSC in 5 channels for each differentiation lineage, and all channels gave positive staining results. We conclude that microfluidic channels support proliferation and differentiation of ADSC. This system uses small amounts of culture medium, experiments with different culture compositions can be efficiently performed, and culture manipulations can be automated using fluid-handling robotics. Because microfluidics can deal with small number of cells, the characteristics of cellular structure and function and the microenvironment of the stem cells can be understood in a more precise manner. The miniaturization of cell culture platforms allows the observation of cellular behaviour at the scale found in living systems.
48
Salipante, Paul F. "Microfluidic techniques for mechanical measurements of biological samples". Biophysics Reviews 4, nr 1 (marzec 2023): 011303. http://dx.doi.org/10.1063/5.0130762.
Pełny tekst źródłaStreszczenie:
The use of microfluidics to make mechanical property measurements is increasingly common. Fabrication of microfluidic devices has enabled various types of flow control and sensor integration at micrometer length scales to interrogate biological materials. For rheological measurements of biofluids, the small length scales are well suited to reach high rates, and measurements can be made on droplet-sized samples. The control of flow fields, constrictions, and external fields can be used in microfluidics to make mechanical measurements of individual bioparticle properties, often at high sampling rates for high-throughput measurements. Microfluidics also enables the measurement of bio-surfaces, such as the elasticity and permeability properties of layers of cells cultured in microfluidic devices. Recent progress on these topics is reviewed, and future directions are discussed.
49
Lai, Xiaochen, Yanfei Sun, Mingpeng Yang i Hao Wu. "Rubik’s Cube as Reconfigurable Microfluidic Platform for Rapid Setup and Switching of Analytical Devices". Micromachines 13, nr 12 (24.11.2022): 2054. http://dx.doi.org/10.3390/mi13122054.
Pełny tekst źródłaStreszczenie:
Microfluidics technology plays an important role in modern analytical instruments, while the modular design of microfluidics facilitates the reconfiguration of analytical instrument functions, making it possible to deploy on-demand systems in the field. However, modular design also faces the challenges such as connection reliability and reconfiguration convenience. Inspired by the self-locking structure of the Rubik’s cube, a modular, reconfigurable microfluidic instrument architecture is proposed in this paper. The system has a self-locking structure of Rubik’s cube components and an O-ring-based alignment and sealing mechanism, which enables reliable interconnection and rapid rearrangement of microfluidic modules by simply rotating the faces of the microfluidic cube. In addition, the system is capable of integrating a variety of customized modules to perform analysis tasks. A proof-of-concept application of detecting multiple pollutants in water is demonstrated to show the reconfigurable characteristics of the system. The findings of this paper provide a new idea for the design of microfluidic analytical instrument architectures.
50
Natu, Rucha, Luke Herbertson, Grazziela Sena, Kate Strachan i Suvajyoti Guha. "A Systematic Analysis of Recent Technology Trends of Microfluidic Medical Devices in the United States". Micromachines 14, nr 7 (24.06.2023): 1293. http://dx.doi.org/10.3390/mi14071293.
Pełny tekst źródłaStreszczenie:
In recent years, the U.S. Food and Drug Administration (FDA) has seen an increase in microfluidic medical device submissions, likely stemming from recent advancements in microfluidic technologies. This recent trend has only been enhanced during the COVID-19 pandemic, as microfluidic-based test kits have been used for diagnosis. To better understand the implications of this emerging technology, device submissions to the FDA from 2015 to 2021 containing microfluidic technologies have been systematically reviewed to identify trends in microfluidic medical applications, performance tests, standards used, fabrication techniques, materials, and flow systems. More than 80% of devices with microfluidic platforms were found to be diagnostic in nature, with lateral flow systems accounting for about 35% of all identified microfluidic devices. A targeted analysis of over 40,000 adverse event reports linked to microfluidic technologies revealed that flow, operation, and data output related failures are the most common failure modes for these device types. Lastly, this paper highlights key considerations for developing new protocols for various microfluidic applications that use certain analytes (e.g., blood, urine, nasal-pharyngeal swab), materials, flow, and detection mechanisms. We anticipate that these considerations would help facilitate innovation in microfluidic-based medical devices.