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1

González, Juan Félix, Teresa Belén Cuello, Antonio José Calderón, Manuel Calderón, Jerónimo González i Diego Carmona. "Cultivation of Autochthonous Microalgae for Biomass Feedstock: Growth Curves and Biomass Characterization for Their Use in Biorefinery Products". Energies 14, nr 15 (28.07.2021): 4567. http://dx.doi.org/10.3390/en14154567.

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In this work, the biomass productivity for biorefinery products and growth curves of three autochthonous microalgae collected in different reservoirs (“Scenedesmus sp.” (SSP), mixture of Scenedesmus sp., Chlorella minutissima, Chlorellas sp. and Nannochloropsis sp. named “La Orden” (LO) consortium and Chlorella minutissima named “Charca Brovales” (CB) consortium) were studied in a 5.5 L column laboratory photobioreactor. Two different culture media, Arnon culture (AM) and an agriculture fertilizer-based liquid medium (FM), have been used to evaluate the growth effect of the microalgae; it was found that the medium has a clear effect on the biomass productivity and growth rate, which ranged between 0.26–0.498 g L−1 d−1 and 0.288–0.864 d−1, respectively. In general, the elemental analysis and higher heating value of microalgae biomass for the three species were independent of the culture medium used for its growth, while their lipids and sugars content depended upon the species type and culture medium used in the cultivation. “La Orden” microalga was selected (given its best adaption to the climatic conditions) to study the biomass productivity and growth rate in two exterior photobioreactors (100 L column and 400 L flat panel), using FM as a medium, obtaining values of 0.116–0.266 g L−1 d−1 and 0.360–0.312 d−1, respectively. An automation and control system was designed to operate the exterior photobioreactors pilot plant. The lipid content of this microalga in these photobioreactors was lower than in the laboratory one, with a fatty acids profile with predominantly palmitic, oleic, linoleic and linolenic acids. Also, the fresh biomass collected from these photobioreactors was studied in a batch type digestion process for biogas production, obtaining a CH4 yield of 296 ± 23 L CH4 kgVSS−1 added with a reduction in percentage of COD and vs. of 50 ± 1% and 50 ± 1.7%, respectively.
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Luche, Daniela Ercole Dale, Bruna Caroline Marques Gonçalves i Messias Borges Silva. "Produção de biomassa da microalga Chlorella minutissima usando mel como fonte de carbono". Brazilian Journal of Development 5, nr 10 (2019): 20531–44. http://dx.doi.org/10.34117/bjdv5n10-239.

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Sonkar, S., i N. Mallick. "Outdoor cultivation of the green microalga Chlorella minutissima under fed-batch mode for biodiesel production". New Biotechnology 44 (październik 2018): S125—S126. http://dx.doi.org/10.1016/j.nbt.2018.05.1060.

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Singh, Vidhu Lekha, Saumita Chakravarty, Neha Chandra i Nirupama Mallick. "Production of sodium copper chlorophyllin from a green microalga Chlorella minutissima: a value-added co-product for sustainable microalgal refinery". Food and Bioproducts Processing 123 (wrzesień 2020): 322–34. http://dx.doi.org/10.1016/j.fbp.2020.07.011.

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Arora, Neha, Alok Patel, Parul A. Pruthi, Krishna Mohan Poluri i Vikas Pruthi. "Utilization of stagnant non-potable pond water for cultivating oleaginous microalga Chlorella minutissima for biodiesel production". Renewable Energy 126 (październik 2018): 30–37. http://dx.doi.org/10.1016/j.renene.2018.03.033.

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Rocha Simões, Patrícia, Jaasiel Espindola Villar, Daniela Ercole Dale Luche, Messias Borges Silva i Bruna Caroline Marques Gonçalves. "Avaliação da atividade antibacteriana do óleo da microalga Chlorella minutissima como alternativa para o tratamento de dermatite atópica". Brazilian Journal of Development 5, nr 11 (2019): 24893–914. http://dx.doi.org/10.34117/bjdv5n11-169.

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Sonkar, Sashi, Dipanwita Deb i Nirupama Mallick. "Outdoor cultivation of the green microalga Chlorella minutissima in mini pond system under batch and fed-batch modes integrating low-dose sequential phosphate addition (LDSPA) strategy for biodiesel production". Biomass and Bioenergy 138 (lipiec 2020): 105596. http://dx.doi.org/10.1016/j.biombioe.2020.105596.

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Sánchez-García, Didier, Anayelli Resendiz-Isidro, Thelma Villegas-Garrido, César Flores-Ortiz, Benjamín Chávez-Gómez i Eliseo Cristiani-Urbina. "Effect of nitrate on lipid production by T. suecica, M. contortum, and C. minutissima". Open Life Sciences 8, nr 6 (1.06.2013): 578–90. http://dx.doi.org/10.2478/s11535-013-0173-6.

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AbstractMicroalgae are an alternative and sustainable source of lipids that can be used as a feedstock for biodiesel production. Nitrate is a good nitrogen source for many microalgae and affects biomass and lipid yields of microalgae. In this study, the effect of nitrate on cell growth and lipid production and composition in Monoraphidium contortum, Tetraselmis suecica, and Chlorella minutissima was investigated. Nitrate affected the production of biomass and the production and composition of lipids of the three microalgae tested. Increasing the nitrate concentration in the culture medium resulted in increased biomass production and higher biomass productivity. Furthermore, increasing the nitrate concentration resulted in a reduction in lipid content and productivity in M. contortum; however, the opposite effect was observed in T. suecica and C. minutissima cultures. C. minutissima and M. contortum lipids contain high levels of oleic acid, with values ranging from 26 to 45.7% and 36.4 to 40.1%, respectively. The data suggest that because of its high lipid productivity (13.79 mg L−1 d−1) and high oleic acid productivity (3.78 mg L−1 d−1), Chlorella minutissima is a potential candidate for the production of high quality biodiesel.
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López Muñoz, Ignacio, i Olivier Bernard. "Modeling the Influence of Temperature, Light Intensity and Oxygen Concentration on Microalgal Growth Rate". Processes 9, nr 3 (9.03.2021): 496. http://dx.doi.org/10.3390/pr9030496.

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Dissolved oxygen plays a key role in microalgal growth at high density. This effect was so far rarely quantified. Here we propose a new model to represent the combined effect of light, oxygen concentration and temperature (LOT-model) on microalgae growth. The LOT-model introduces oxygen concentration in order to represent the oxidative stress affecting the cultures, adding a toxicity term in the expression of the net growth rate. The model was validated with experimental data for several species such as Chlorella minutissima, Chlorella vulgaris, Dunaliella salina, Isochrysis galbana. It successfully predicted experimental records with an average error lower than 5.5%. The model was also validated using dynamical data where oxygen concentration varies. It highlights a strong impact of oxygen concentration on productivity, depending on temperature. The model quantifies the sensitivity to oxidative stress of different species and shows, for example, that Dunaliella salina is much less affected than Chlorella vulgaris by oxidative stress. The modeling approach can support an optimization strategy to improve productivity, especially for managing high oxygen levels.
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Andriopoulos, Vasilis, Maria D. Gkioni, Eleni Koutra, Savvas G. Mastropetros, Fotini N. Lamari, Sophia Hatziantoniou i Michael Kornaros. "Total Phenolic Content, Biomass Composition, and Antioxidant Activity of Selected Marine Microalgal Species with Potential as Aquaculture Feed". Antioxidants 11, nr 7 (4.07.2022): 1320. http://dx.doi.org/10.3390/antiox11071320.

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There has been growing interest in microalgal biomolecules for health and cosmetics, as well as in the use of microalgae as aquaculture feed due to the need to replace fishmeal and fish oil with sustainable yet equally nutritious alternatives. Aim of this study is to evaluate the potential of five marine microalgal species, namely Chlorella minutissima, Dunaliella salina, Isochrysis galbana, Nannochloropsis oculata and Tisochrysis lutea, for the co-production of antioxidants and aquaculture feed. Batch cultivation was performed under saturating light intensity and continuous aeration. Freeze-dried biomass was extracted sequentially with water and methanol and evaluated for phenolic content and antioxidant activity, as well as proximate composition and fatty acid profile. Methanolic extracts of C. minutissima presented the highest phenolic content, measured with the Folin–Ciocalteu assay, and antioxidant activity. However, HPLC and LC-MS showed the presence of non-pigment compounds only in T. lutea. Total phenolic content and antioxidant activity were correlated to chlorophyll content. N. oculata and T. lutea were rich in eicosapentaenoic acid and docosahexaenoic acid, respectively, as well as in protein. In conclusion, N. oculata and T. lutea are suitable candidates for further optimization, while the data presented suggest that pigment effects on the Folin–Ciocalteu method require reconsideration.
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SHARMA, GULSHAN KUMAR, SHAKEEL A. KHAN, MANOJ SHRIVASTAVA, N. GUPTA, SANDEEP KUMAR, LAL CHAND MALAV, MAHAVEER NOGIYA i S. K. DUBEY. "Bioremediation of sewage wastewater through microalgae (Chlorella minutissima)". Indian Journal of Agricultural Sciences 90, nr 10 (4.12.2020): 2024–28. http://dx.doi.org/10.56093/ijas.v90i10.107985.

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Phycoremediation is a cost effective, environmentally sustainable, safe and alternative technology for remediation of wastewater. The present work was aimed to evaluate the role of inoculated microalgae (Chlorella minutissima) in phycoremediation of sewage wastewater. The selected microalgae removed 94.4% TDS, 88.9% of NO3 -N, 66.3% potassium, 67.4% phosphorus, 48.2% NH4 +, 93% (Biological Oxygen Demand) BOD5 and 80.5% (Chemical Oxygen Demand) COD. The wastewater obtained after phycoremediation have safe limit of nutrients to be used as irrigation water in agricultural fields. The results of this study suggested that growing algae in nutrient-rich sewage wastewater offers a new opportunity to bio-remediate pollution load of wastewater and use it for irrigation purposes.
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Tang, Haiying, Meng Chen, M. E. D. Garcia, Nadia Abunasser, K. Y. Simon Ng i Steven O. Salley. "Culture of microalgae Chlorella minutissima for biodiesel feedstock production". Biotechnology and Bioengineering 108, nr 10 (22.07.2011): 2280–87. http://dx.doi.org/10.1002/bit.23160.

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Ben Atitallah, Ali, Faiez Hentati, Mouna Dammak, Bilel Hadrich, Imen Fendri, Mohamed-Ali Ayadi, Philippe Michaud, Slim Abdelkafi i Mohamed Barkallah. "Effect of Microalgae Incorporation on Quality Characteristics and Functional and Antioxidant Capacities of Ready-to-Eat Fish Burgers Made from Common Carp (Cyprinus carpio)". Applied Sciences 9, nr 9 (2.05.2019): 1830. http://dx.doi.org/10.3390/app9091830.

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Microalgae have been used as natural ingredients to produce functional and nutritional food products. The impact of the addition of Chlorella minutissima, Isochrysis galbana, and Picochlorum sp. at concentrations of 0.5, 1, and 1.5% w/v on the texture and sensory attributes of canned burgers were investigated. The results show that carp formulations containing 1% microalgae show significantly better classification performance for many textural and sensory parameters compared to the rest of the formulations. Also, these treatments had higher swelling ability as well as water and oil holding capacities, thanks to the important dietary fiber and polysaccharide contents found in microalgae. Moreover, microalgae-supplemented burgers were characterized as having low a* and b* values, which made the color appear to be pale orange. Additionally, thanks to its richness in pigments and polysaccharides, microalgae considerably ameliorated the antioxidant activities of the new prepared fish burgers. Thus, microalgae could be used as natural and nutritious ingredient to develop new fish-based products.
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Raghuwanshi, Navdeep, Neha Arora, Ritu Varshney, Partha Roy i Vikas Pruthi. "Antineoplastic and antioxidant potential of phycofabricated silver nanoparticles using microalgae Chlorella minutissima". IET Nanobiotechnology 11, nr 7 (28.07.2017): 827–34. http://dx.doi.org/10.1049/iet-nbt.2016.0201.

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Hawrot-Paw, Małgorzata, Adam Koniuszy, Małgorzata Gałczyńska, Grzegorz Zając i Joanna Szyszlak-Bargłowicz. "Production of Microalgal Biomass Using Aquaculture Wastewater as Growth Medium". Water 12, nr 1 (29.12.2019): 106. http://dx.doi.org/10.3390/w12010106.

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Aquaculture wastewater contains a huge amount of substances that can cause environmental pollution. However, microalgae can absorb these compounds and convert them into useful biomass. In this study, Chlorella minutissima was grown in the wastewater resulting from saline aquaculture. The microalgae were found to effectively utilize nitrogen and phosphorus in the wastewater for its growth. During wastewater treatment, the cell density increased almost fivefold compared to the initial value (OD680 0.502). Moreover, batch culture resulted in the maximum biomass concentration and productivity of 4.77 g/L and 0.55 g/L/day, respectively. The contents of total nitrogen and total phosphorus in wastewater decreased by 88% and over 99%, respectively. In addition, the content of N-NO3 was reduced by 88.6%, N-NO2 by 74.3%, and dissolved orthophosphates (V) by 99%. At the beginning and throughout the experiment, the content of N-NH4 in wastewater remained below 0.05 mg/L. Furthermore, a high lipid content of 46.4% (w/w) was also obtained from the studied microalgae.
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Sharan, Abhishek, i Seema Nara. "Exposure of synthesized Co3O4 nanoparticles to Chlorella minutissima: An ecotoxic evaluation in freshwater microalgae". Aquatic Toxicology 224 (lipiec 2020): 105498. http://dx.doi.org/10.1016/j.aquatox.2020.105498.

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Klin, Marek, Filip Pniewski i Adam Latała. "Characteristics of the growth rate and lipid production in fourteen strains of Baltic green microalgae". Oceanological and Hydrobiological Studies 47, nr 1 (26.03.2018): 10–18. http://dx.doi.org/10.1515/ohs-2018-0002.

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Abstract Screening of fourteen Baltic green algal strains provided basic data on their mass culture potential for the purpose of valuable biomass production with particular emphasis on lipid content. Selected microalgae were grown under non-stressed conditions in order to identify those characterized by efficient lipid production. The tested strains exhibited significant differences in growth patterns and lipid yields. Strains belonging to Chlorella and Stichococcus genera exhibited the highest growth rates, ranging from 0.39 d-1 to 0.50 d-1 and thus the highest final cell density (> 107 cells ml-1). Furthermore, five strains: C. minutissima BA-12, C. fusca BA-18, C. vulgaris BA-80, Monoraphidium sp. BA-165 and Chlorella sp. BA-167 were characterized by distinctively high lipid yield (> 60 mg l-1). The same strains, together with C. vulgaris BA-02, were also shown as those with the highest volumetric lipid productivity, reaching > 30 mg l-1 d-1. The tested Baltic strains performed well in terms of lipid production compared to the literature data, still leaving a great spectrum of opportunities for further lipid yield improvement.
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Amaral, M. S., C. C. A. Loures, F. L. Naves, B. E. L. Baeta, M. B. Silva i A. M. R. Prata. "Evaluation of cell growth performance of microalgae Chlorella minutissima using an internal light integrated photobioreactor". Journal of Environmental Chemical Engineering 8, nr 5 (październik 2020): 104200. http://dx.doi.org/10.1016/j.jece.2020.104200.

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Sharma, Amit Kumar, Abhishek Sharma, Yashvir Singh i Wei-Hsin Chen. "Production of a sustainable fuel from microalgae Chlorella minutissima grown in a 1500 L open raceway ponds". Biomass and Bioenergy 149 (czerwiec 2021): 106073. http://dx.doi.org/10.1016/j.biombioe.2021.106073.

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Yang, JinShui, Jing Cao, GuanLan Xing i HongLi Yuan. "Lipid production combined with biosorption and bioaccumulation of cadmium, copper, manganese and zinc by oleaginous microalgae Chlorella minutissima UTEX2341". Bioresource Technology 175 (styczeń 2015): 537–44. http://dx.doi.org/10.1016/j.biortech.2014.10.124.

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Georgakis, Nikolaos D., Elisavet Ioannou, Marianna Chatzikonstantinou, Marián Merino, Evangelia G. Chronopoulou, José Luis Mullor, Panagiotis Madesis i Nikolaos E. Labrou. "The Cosmeceutical Potential of the Yellow-Green Alga Trachydiscus minutus Aqueous Extract: Preparation of a Natural-Based Dermal Formula as a Proof of Concept". Cosmetics 10, nr 3 (10.05.2023): 75. http://dx.doi.org/10.3390/cosmetics10030075.

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In the present study, selected cosmeceutical properties of aqueous extracts from the microalgae strain Trachydiscus minutus were assessed and compared with those obtained using three widely used Chlorella strains (C. vulgaris, C. sorokiniana, and C. minutissima). Among all extracts, T. minutus extracts showed the highest total antioxidant capacity (TAC) and inhibitory potency towards elastase, suggesting potential activity in controlling skin aging. Furthermore, the cytotoxicity, anti-inflammatory activity and UVA protection of T. minutus extract were evaluated employing normal human dermal fibroblasts (NHDF) and human keratinocyte HaCaT cells. The results showed that the T. minutus extract was able to significantly inhibit the transcription of selected marker genes involved in inflammation [interleukin-6 (IL-6), interleukin-8 (IL-8) and tumor necrosis factor alpha (TNFα)]. In addition, treatment of NHDF and HaCaT cells with T. minutus extract ameliorate the UVA-induced cell damage by decreasing the accumulation of reactive oxygen species (ROS). Extracts from T. minutus were formulated into a skin care cream and an aqueous gel. Both formulas exhibited excellent compatibility and stability. Comprehensively, all these results suggest that T. minutus extract displays promising cosmeceutical properties by providing antioxidant, anti-aging, and anti-inflammatory activities, and therefore has potential for cosmeceutical use.
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D'Alessandro, Emmanuel Bezerra, Ina De Souza Nogueira i Nora Katia Saavedra del Aguila Hoffmann. "Variability in phytoplankton community structure and influence on stabilization pond functioning". Ambiente e Agua - An Interdisciplinary Journal of Applied Science 15, nr 2 (8.04.2020): 1. http://dx.doi.org/10.4136/ambi-agua.2507.

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Density of phytoplankton in stabilization ponds influences treatment performance. The study hypothesized that the phytoplankton community structure varies according to local and temporal changes and consequently influences the treatment of the effluent. Phytoplankton community structure in facultative and maturation ponds in central Brazil was analyzed to provide guidance on system operation and maintenance. Further, species density, abundance, diversity, richness, dominance and beta diversity were measured. The efficiency of the treatment was evaluated based on Biochemical Oxygen Demand (BOD). Additionally, a Canonical Correspondence Analysis (CCA) was used to investigate how physical and chemical variables influenced the composition of the most abundant species in the dry and rainy seasons and the microalgae that were most related to the removal of BOD. One hundred and sixty-eight taxa were recorded, and the most abundant classes in both ponds were Chlorophyceae and Cyanophyceae (40% potentially toxic). The maturation pond had greater adaptability in the rainy season, while the facultative pond was more flexible in the dry season. The best period of growth varied among species. In both ponds, Chlorella minutissima was the one which most contributed to the optimization of the treatment. Thus, identifying phytoplankton species and relating them to water quality parameters and weather can help to understand the ecological dynamics of wastewater treatment and provide useful information for the operation and maintenance of stabilization ponds.
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Kotzabasis, K., A. Hatziathanasiou, M. V. Bengoa-Ruigomez, M. Kentouri i P. Divanach. "Methanol as alternative carbon source for quicker efficient production of the microalgae Chlorella minutissima: Role of the concentration and frequence of administration". Journal of Biotechnology 70, nr 1-3 (kwiecień 1999): 357–62. http://dx.doi.org/10.1016/s0168-1656(99)00088-7.

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Makridis, Pavlos, Rita Alves Costa i Maria Teresa Dinis. "Microbial conditions and antimicrobial activity in cultures of two microalgae species, Tetraselmis chuii and Chlorella minutissima, and effect on bacterial load of enriched Artemia metanauplii". Aquaculture 255, nr 1-4 (maj 2006): 76–81. http://dx.doi.org/10.1016/j.aquaculture.2005.12.010.

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G, Saxena, i Kumar L. "Identification of Potential Culture Conditions for Enhancing the Biomass Production of Microalga Chlorella minutissima". Expert Opinion on Environmental Biology s1 (2016). http://dx.doi.org/10.4172/2325-9655.s1-005.

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Sonkar, Sashi, Shibani i Nirupama Mallick. "A machine learning approach in drying of microalga Chlorella minutissima in a single rotary drum dryer for biodiesel production". Environmental Progress & Sustainable Energy, 16.11.2021. http://dx.doi.org/10.1002/ep.13786.

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Abo-Elmagd, Rasha A., Ragaa A. Hamouda i Mervat H. Hussein. "Phycotoxicity and catalytic reduction activity of green synthesized Oscillatoria gelatin-capped silver nanoparticles". Scientific Reports 12, nr 1 (27.11.2022). http://dx.doi.org/10.1038/s41598-022-22976-6.

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AbstractOver the last decade, an extensive range of consumer products containing manufactured silver nanoparticles (AgNPs) have been progressively used. The unfitting usage and discharge of these materials can enable passage of AgNPs into the aquatic ecosystem causing prospective toxicological consequence. The present study shed new lights on the phycotoxicity of small (8.47–17.66 nm) and stable Oscillatoria reduced gelatin-capped silver nanoparticles (OG-AgNPs) fabricated using a completely green synthetic technique. In this work, estimating of the possible toxic effects of OG-AgNPs on two freshwater microalgae Chlorella vulgaris and Chlorella minutissima was carried. This study found that, the growth of cells and photosynthetic pigment inhibitory effects of OG-AgNPs exhibit a significant increase with increasing time and concentration compared to control. Based on the IC50 value C. vulgaris (3.705 μg/mL) was found to be more sensitive to OG-AgNPs than C. minutissima (5.8 μg/mL). This study revealed that OG-AgNPs exhibit potent phycotoxic effect against Chlorella species. Finally, the negative effect of OG-AgNPs on aquatic algae and these modifications might have severe effects on structure and function of aquatic ecosystems. Besides, the biosynthesized OG-AgNPs showed a catalytic activity in the reduction of hydrogen peroxide, one of the reactive oxygen species that represent a major threat to biological systems. This method pretends an auspicious non-skill dependent technique with a good sensitivity for determination of H2O2 concentration, particularly at trace ppm level for applying in numerous domains such as medical and industrial processes.
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Balkanlı, Nihat Erdem, İbrahim Işıldak, Benan İnan, Tuğba Özer i Didem Özçimen. "Monitoring microalgal growth of Chlorella minutissima with a new all solid‐state contact nitrate selective sensor". Biotechnology Progress, 6.03.2022. http://dx.doi.org/10.1002/btpr.3247.

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